From owner-peptides@net.bio.net Tue Jun 01 10:52:00 1999 Path: biosci!biosci!not-for-mail From: Maria Jesus Martin Newsgroups: bionet.molecules.peptides Subject: Release 10 of TrEMBL, a protein sequence database supplementing Date: 1 Jun 1999 04:52:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 96 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7j0hi0$9m7@net.bio.net> NNTP-Posting-Host: net.bio.net INTRODUCTION ============ TrEMBL is a protein sequence database supplementing the SWISS-PROT Protein Sequence Data Bank. TrEMBL contains the translations of all coding sequences (CDS) present in the EMBL Nucleotide Sequence Database not yet integrated in SWISS-PROT. TrEMBL can be considered as a preliminary section of SWISS-PROT. For all TrEMBL entries which should finally be upgraded to the standard SWISS-PROT quality, SWISS-PROT accession numbers have been assigned. RELEASE 10.0 OF TrEMBL ===================== This TrEMBL release is created from the EMBL Nucleotide Sequence Database release 58 and contains 244'862 sequence entries, comprising 66'562'800 amino acids. TrEMBL is split in two main sections; SP-TrEMBL and REM-TrEMBL: SP-TrEMBL (SWISS-PROT TrEMBL) contains the entries (201'082), which should be eventually incorporated into SWISS-PROT. SWISS-PROT accession numbers have been assigned for all SP-TrEMBL entries. SP-TrEMBL is organized in subsections: arc.dat (Archea): 7408 entries fun.dat (Fungi): 6679 entries hum.dat (Human): 8518 entries inv.dat (Invertebrates): 23653 entries mam.dat (Other Mammals): 3130 entries mhc.dat (MHC proteins): 4236 entries org.dat (Organelles): 16261 entries phg.dat (Bacteriophages): 1971 entries pln.dat (Plants): 17352 entries pro.dat (Prokaryotes): 45992 entries rod.dat (Rodents): 7480 entries unc.dat (Unclassified): 44 entries vrl.dat (Viruses): 53916 entries vrt.dat (Other Vertebrates): 4442 entries REM-TrEMBL (REMaining TrEMBL) contains the entries (46'785) that we do not want to include in SWISS-PROT. WEEKLY UPDATES OF TrEMBL AND NON-REDUNDANT DATA SETS ==================================================== Weekly cumulative updates of TrEMBL are available by anonymous FTP and from the EBI SRS server. We also produce every week a complete non-redundant protein sequence collection by providing three compressed files (these are in the directory /pub/databases/sp_tr_nrdb on the EBI FTP server): sprot.dat.Z, trembl.dat.Z and trembl_new.dat.Z. ACCESS/DATA DISTRIBUTION ======================== FTP server: ftp.ebi.ac.uk/pub/databases/trembl SRS server: http://srs.ebi.ac.uk/ TREMBL is also available on the SWISS-PROT CD-ROM. SWISS-PROT + TREMBL is searchable on the FASTA3, BLAST2 and Bic_sw servers of the EBI. TrEMBL HAS BEEN PREPARED BY: ============================ Rolf Apweiler, Kirsty Bates, Margaret Biswas, Sergio Contrino, Wolfgang Fleischmann, Gill Fraser, Henning Hermjakob, Vivien Junker, Youla Karavidopoulou, Fiona Lang, Minna Lehvaslaiho, Michele Magrane, Maria Jesus Martin, Steffen Moeller, Nicoletta Mitaritonna, Nicola Mulder, Claire O'Donovan and Eleanor Whitfield at the EMBL Outstation - European Bioinformatics Institute (EBI) in Hinxton, UK; Amos Bairoch and Alain Gateau at the Swiss Institute of Bioinformatics in Geneva, Switzerland. ----------------------------------------------------------------- Maria Jesus Martin email:martin@ebi.ac.uk EMBL Outstation EBI (European Bioinformatics Institute) URL: http://www.ebi.ac.uk Wellcome Trust Genome Campus Tel: +44 (1223) 494408 Hinxton fax: +44 (1223) 494468 Cambridge CB10 1SD UK ----------------------------------------------------------------- From owner-peptides@net.bio.net Tue Jun 08 12:13:00 1999 Path: biosci!biosci!not-for-mail From: Per Thoren Newsgroups: bionet.molecules.peptides Subject: Stirring bar NOT made of PTFE Date: 8 Jun 1999 06:13:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7jj4ti$7vv@net.bio.net> NNTP-Posting-Host: net.bio.net Has anyone come across stirring bars that are NOT made of PTFE, but of e.g. glass or PS? I would like to graft PEG to the surface and that seems impossible with the PTFE coating that is standard. Per Thorén Dept. of Physical Chemistry Göteborg Sweden From owner-peptides@net.bio.net Tue Jun 08 19:05:00 1999 Path: biosci!biosci!not-for-mail From: Michael R. Thompson Newsgroups: bionet.molecules.peptides Subject: Re: Stirring bar NOT made of PTFE Date: 8 Jun 1999 13:05:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7jjt1h$fjq@net.bio.net> NNTP-Posting-Host: net.bio.net I used to make my own micro-stir bars when I needed one: take some soft (i.e. non-pyrex) glass tubing, score and break to the length needed, insert an appropriate length of iron wire (e.g. a piece of straightened paper-clip) then fuse the ends of the glass tube in a bunsen. These are non-magnetic themselves but work fine on a standard stir plate with magnetic impeller. Per Thoren wrote in message <7jj4ti$7vv@net.bio.net>... >Has anyone come across stirring bars that are NOT made of PTFE, but of e.g. glass or PS? I would like to graft PEG to the surface and that seems impossible with the PTFE coating that is standard. > >Per Thorin >Dept. of Physical Chemistry >Gvteborg >Sweden > > From owner-peptides@net.bio.net Thu Jun 10 17:48:00 1999 Path: biosci!biosci!not-for-mail From: BILONG Transgenics Newsgroups: bionet.molecules.peptides Subject: '99 ISAAST. Date: 10 Jun 1999 11:48:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 254 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7jp19t$1jl@net.bio.net> NNTP-Posting-Host: net.bio.net '99 International Symposium on Aging and Antiaging Science & Technology (with EXHIBITION) September 8-12, 1999 Beijing, China Scientific Sessions 1. Biological Science 2. Clinical & Experimental Medicine 3. Health Care for the Elderly 4. Traditional Chinese Medicine in Aging & Antiaging Invited Speakers: I. Biological Sciences Prof. Hajime Orimo, Japan # Osteoporosis - - - -Update *Dr. Dazhong Yin, Sweden # Free Radical, Non-enzymatic Glycosylation & Lipofuscin Prof. Zong-Yu Zhang and Tan-Jun Tong: Beijing, China # Progress in Research on Molecular Mechanisms of Aging Dr. Xu Song, Beijing, China # The Demonstration of the Thesis of Nonenzymatic Glycosylation Inducing Aging *Prof. Ayala Hochman, Tel Aviv, Isreal, £¿ # Overview of Free Radicals and Neurodegenerative diseases * Dr. Ron Kohen, Jerusalem, Israel # Reducing Equivalents in the Aging Process Prof. Beka Solomon: Tel Aviv, Isreal # Therapeutic Antibodies, a New Approach in Treatment of Alzheimer’s Disease Prof. Wen-Bin Li, Beijing, China # Aging-mimetic effect of D-galactose and its Mechanism Dr. Zhong-Ming Wen, Suzhou, China Study of Aging Model in Rats Prof. Biansheng Liu, Wuhan, China # The Relationship of Nutrition and Aging Prof. Anna Xue, Beijing, China # Study on the Induction of Apoptosis by Superoxide Anion and Inhibition Effect of Antioxidant Nutrient Dr. Andrey I. Sukhodub, Kharkiv, Ukraine # Hypothetical Mechanism of Benzene Membrane Damage Action on the Liver Microsomers£¨£¿£© from Different Age Rats Prof. Ke-ji Chen, Beijing, China # Chinese Medicine and Health Preserving Dr. Cai-min Xu, Beijing, China # The Changes of Membrane Lipids and Proteins in the Aging Process of Erythrocytes Dr. Ping Wang, Wuhan, China # Theoretical and Experimental Research on the Relationship Between Excessive Intake of Fat-sweet Food and Senility II. Geriatrics *Prof. David J.E.Callaway, New York, USA # Molecular Model of Alzheimer Amyloid Fibril Formation Prof. Rui Han, Beijing, China # Recent Progress of Anticancer Drugs Originated From Plants in China Prof. Shu-Li Sheng, Beijing, China # Advance in Pathogenic Mechanism of Alzheimer’s Disease Prof. Yong-Jie Li, Beijing, China # Progress in Surgical Treatment of Parkinson’s Disease Dr. Jinghe Li, Pharmacia & Upjohn, USA # A New Twist in Alzheimer Presenilins Prof. Geng-tao Liu, Beijing, China # Protective Action of the Extract of Gingko Biloba (EGb761) Against Oxidized Low Density Lipoprotein-induced Damage of Endothelial Cells of New Born Calf Aorta. Dr. Shi-xin Jin, Beijing, China # The Osteoporosis of Aged Male --74 Case Analysis Prof. Yong-xing Ma, Shanghai, China # Cognitive Impairment and Dementia in the Aged and Preaged : Comprehensive Mechanism and Intervention Prof. Zhu-fang Shen, Beijing, China # The Hypoglycemic Effects of a Chinese Traditional Medicine (S 2), One of the Glucosidase Inhibitors Dr. Chun-qiang Guo, Beijing, China # Effects of 9804 on Learning and Memory of Mice Dr. Ying Wang, Beijing, China # Investigation of 9804 on Some Amino Acids in Brain of Mice Dr. Jiang-guo Chen, Nanjing, China # Studies on the Relationship between the Oxidatively Modified Low Density Lipoprotein and the Aging of the Myocardial and Renal Cells in Ovariectomized Rhesus Monkey. Dr. Huaxi Xu, New York, USA # Cell Biology of Alzheimer’s Disease: Mechanism for Beta-amyloid Generation and Regulation Dr. Lan Sun, Beijing, China # Effects of Coryfolia Extract in Prevention and Treatment of Osteoporosis Dr. Feng-hua Liu, Beijing, China # The Neuprotective Effect of Estrogen and Antioxidants Against Beta-amyloid Peptides(25-35) Induced Toxicity in Cultured Neuron Cells Dr. Wei Wang, Beijing, China # Research Report on Behavior and Therapeutic Mechanism of Alzheimer’s-type Dementia Induced in Mice by Centrally Administered Beta-amyloid Peptides Yan Chen, Beijing, China # Therapeutic Effects of 764-3 on Alzheimer’s-type Dementia Induced in Rats by Centrally Administered Beta-amyloid Peptides Dr. Mengen Zhang, Beijing, China # Prevention and Therapeutic Effects of BuShenHuoXueFang on Ischemia-type Dementia in Mice Hu Zhu, Beijing, China # Combined Monitoring of CEA and TSA Value in Early Diagnosis of Lung cancer III. Traditional Chinese Medicine in Treatment of Diseases in the Elderly Dr. Jia-shi Zhu, California, USA # The Effect of Chinese Anti-aging Food/drug Corcyceps (CordyMaxTM Cs-4) Improves Steady Bio-energy Status in Mouse Liver Prof. Jun-tian Zhang, Beijing, China # Study on the Antiaging Effect of Ginsenoside Rg1 and Rb1 Prof. Junda Liu, Beijing, China # Modulate effect of Chinese Herbs on Immune Function of Lymphocytes in the Elderly Prof. Chunsheng Li, Beijing, China # Status of a Decade of Study on Anti-senility Drugs by Applying Life-experiment and Senile Animal Model in China Zhi-xin Li, Beijing, China # Comparative Study on Modulating Effects of Different Kidney-Supplementing Spleen-vigorating Stasis-Removing Decoctions on Immune Function and Free Radical Metabolism in Old Mice Prof. Zhi-qui Wu, Beijing, China #1. Molecular Study of Crystalline Modification for Preventing Senile Cataract with Chinese Herbs of Tonifying Kidney and Supplementing Blood #2. A Study of Antiaging by Chinese Medicine Herbs of Tonifying Kidney and Nurishing Blood from the View of TCM theory. Dr. Guan-hua Du and Yong-hong Chen, Beijing, China # Effects of Salvianolic acid B on the Functions of Brain Mitochondria in Aging Mice Prof. Lin Li, Beijing, China # Effects of Chinese Herbs on dementia-like animal Models and Cell Models Prof. Jin-Zhou Tian, Beijing, China #1. The Influence of Compound Fhubarb Preparation on ChAT, AChE Activities and Ach Content of Aged Mice’s Cerebral Cortex and Hippocampus. or #2. Neuropsychological Findings Preceding Alzheimer’s dementia in English Individuals with Cognitive Impairment. #3. Research into the Treatment for Vascular Dementia in China Using Traditional Chinese Therapies Dr. Jiansheng Li, Zhengzhou, China # Effect of Daihuang Zhechong Pill of TCM on Kidney TXB2 and 6-Keto-PGF12 of Patients with Early-Stage Diabetic Nephropathy in the Aged. Exhibition The companies engaged in producing the instruments or reagents that may facilitate the antiaging researches are welcomed and encouraged to exhibit their high-tech products. For whose interested in the exhibition, please contact us at your earliest convenience. Sponsor Antiaging Science & Technology Society£¬ Gerontological Society of China Organizers Antiaging Science & Technology Society , Gerontological Society of China China International Symposium Center for Sciences and Technology (CICCST) Supporters Gerontological Society of China Institute of Medicinal Biotechnology, Peking Union Medical College, Chinese Academy of medical Sciences Beijing Hospital and Gerontological Research Institute (to be continued) Contact: ********************************************************* BILONG Academic Events Add: 8 Nan Er Jie, Zhong Guan Cun, Beijing 100080, China Box: P.O. Box 8734, Beijing 100080, China Tel: 86-10-6256-0561, 6256-2226 Ext 211, 800-810-0797 Fax: 86-10-6253-2114 Email: BAE@bilong.com Website: www.ciccst.org.cn/99ISAAST ********************************************************* From owner-peptides@net.bio.net Thu Jun 10 21:42:00 1999 Path: biosci!biosci!not-for-mail From: "Achim Recktenwald, PhD" Newsgroups: bionet.molecules.peptides Subject: Re: Stirring bar NOT made of PTFE Date: 10 Jun 1999 15:42:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7jpf0v$3g9@net.bio.net> NNTP-Posting-Host: net.bio.net The company Fisher Scientific sells a glass-covered magnetic bar; taken from their catalog at http://www.fischersci.com: "Magnet sealed in glass. 7/16 diameter x 1 3/16 in. long (11 x 30mm)." Cheers, Achim Http://www.StressGen.com Per Thoren wrote in message news:7jj4ti$7vv@net.bio.net... > Has anyone come across stirring bars that are NOT made of PTFE, but of e.g. glass or PS? I would like to graft PEG to the surface and that seems impossible with the PTFE coating that is standard. > > Per Thorén > Dept. of Physical Chemistry > Göteborg > Sweden > > From owner-peptides@net.bio.net Mon Jun 14 12:06:00 1999 Path: biosci!biosci!not-for-mail From: The Antibody Resource Page Newsgroups: bionet.molecules.peptides Subject: How to find anti-peptide antibodies: The Antibody Resource Page Date: 14 Jun 1999 06:06:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7k2uoi$ih0@net.bio.net> NNTP-Posting-Host: net.bio.net The Antibody Resource Page (http://www.antibodyresource.com/) is your number one source for information on antibodies. Here is just some of what can be found on the page: 1. Custom Antibodies - a list of online companies that will make custom antibodies for you. 2. Books - a growing section on books important to researchers who work with antibodies. Do you sell such books? Send us the book and we can post a review. 3. How to Find an Antibody - a variety of ways on and off the web to find the antibody you are looking for. There are links to free search engines that allow you to peruse a multitude of companies for the specific antibody that you need. 4. Online Companies - links to over 150 companies that sell antibodies or antibody related products. Is your company listed on this page? 5. Antibody Image Gallery - see some of the latest in animated and non-animated antibody graphics 6. Bulletin Board - Have a question? Then stop by and post a message. Or answer a question. 7. Educational Resources - a variety of new links have been added.There are links to pages on immunochemistry, antibody production, autoimmunity, vaccines, immunology and much more. This page is divided up into sections on research, educational, and health resources. 8. Antibody News - Get up-to-date, antibody-related news articles and newsgroup discussions 9. Online Databanks and Databases - links to online protein and nucleic acid sequencing databases. Invaluable to biochemists and molecular biologists who work with antibodies. See it all at: http://www.antibodyresource.com/ antibody@antibodyresource.com ps. Don't forget to visit our new contract antibody services page. It is designed especially for scientists in industry who want to outsource the large scale production of antibodies. If you know of a company not on our list, please email us. From owner-peptides@net.bio.net Mon Jun 14 15:14:00 1999 Path: biosci!biosci!not-for-mail From: arthur roberts Newsgroups: bionet.molecules.peptides Subject: Information on Biochemistry Date: 14 Jun 1999 09:14:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7k39o8$fei@net.bio.net> NNTP-Posting-Host: net.bio.net Art's Biotechnology Resource ---------------------------- The website can be linked by the following: http://biotech.isCool.net or http://www.ahpcc.unm.edu/~aroberts or http://www.arc.unm.edu/~aroberts This site allows easy access to hyperlinks and free software for the biochemist, biophysicist and molecular biologist. This site is constantly evolving and expanding, so it can be easy to use, reliable, and comprehensive. As of 6/13/99, many new links have been added. There have also been some new categories added: Employment Opportunities: this is a list of the "searchable" databases with jobs that are related to the biotechnology field. If you know of any links that are related to employment, please email me at arthurr@wsunix.wsu.edu Best Biotechnology Sites: this is a list of the best biotechnology- related sites in the world. If you know of any websites that are not on the list that would be considered the best, please email me at arthurr@wsunix.wsu.edu Bioethics/ Legal Issues: provides information on the public's view of biotechnology research and legal issues that concern the biotechnology community. If you know of any other important sites related to the legality of biotechnology or ethics (i.e. human cloning), please email me at arthurr@wsunix.wsu Sincerely, Art Roberts (web designer) arthurr@wsunix.wsu.edu (P.S. I reply to all emails that are sent, and I appreciate your comments and suggestions) From owner-peptides@net.bio.net Tue Jun 15 17:13:00 1999 Path: biosci!biosci!not-for-mail From: gregg@microcosm.com Newsgroups: bionet.molecules.peptides Subject: Doctoral R&D Position Available in Photonics and Imaging Date: 15 Jun 1999 11:13:27 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7k6547$44p@net.bio.net> NNTP-Posting-Host: net.bio.net Ph.D. graduates in physics, biophysics, material sciences or related areas are encouraged to apply for a post doctoral position in the-state-of-the-art time-resolved imaging laboratory at Microcosm, Inc. The successful candidate will be part of a team developing photonics technologies for solid state and biomedical applications. The knowledge and hands-on experience with femtosecond lasers, time-resolved fluorescence spectroscopy and imaging instrumentation is essential. This position is only for US citizens. If you are interested please send your resume to Dr. Henryk Malak via either fax at (301) 725-2941 or email at henryk@microcosm.com. Dr. Henryk Malak Sent via Deja.com http://www.deja.com/ Share what you know. Learn what you don't. From owner-peptides@net.bio.net Tue Jun 22 06:03:00 1999 Path: biosci!biosci!not-for-mail From: Per Mygind Newsgroups: bionet.molecules.peptides Subject: Surfacelabelling of (Bacterial) proteins Date: 22 Jun 1999 00:03:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 39 Sender: daemon@net.bio.net Approved: cmgross@biosci.cbs.umn.edu Distribution: world Message-ID: <7kncf4$5tq@net.bio.net> NNTP-Posting-Host: net.bio.net Hello I'm just wondering wether any of you are aware of any methods for detection of surfaceexposed proteins in bacteria, apart from: Biotinylation Limited protease digestion Antigendetection by an antibody (EM, IMF) Surface-iodination Any other suggestions as to how to detect the proteins present on the surface of a given microorganism -- Regards Per Mygind ************************************************************************ If you are are not part of the solution, you are part of the precipitate ************************************************************************ Per Mygind, Cand.scient Department of Medical Microbiology and Immunology The Bartholin Building, University of Aarhus, Denmark phone : 89 42 17 47, fax : 86 19 61 28 ************************************************************************ It's hard work and great art to make life not so serious ************************************************************************ Life is what happens to you while you're busy making other plans (John Lennon) All you touch and all you see is all your life will ever be (Roger Waters)