From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!uknet!daresbury!not-for-mail From: AW14@VAX.YORK.AC.UK Newsgroups: bionet.plants Subject: potassium economies Message-ID: <2d50g0$t5o@mserv1.dl.ac.uk> Date: 26 Nov 93 13:31:44 GMT Sender: daemon@mserv1.dl.ac.uk Distribution: bionet Lines: 6 Original-To: PLANTBIO@dl.AC.UK Hello world ! Is there anyone out there working on the potassium economy of the potato or any other plant ? I would particularly like to know of any work relating potassium to internal bruising in these friendly tubers. Thanks in advance, Angela Wilson, University of York aw14@uk.ac.york.vax From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!emba-news.uvm.edu!brianf From: brianf@med.uvm.edu (Brian Foley) Newsgroups: bionet.molbio.methds-reagnts,bionet.cellbiol,bionet.plants Subject: Re: JANE LANCASTER Message-ID: <1993Dec2.202643.19924@emba.uvm.edu> Date: 2 Dec 93 20:26:43 GMT References: <1993Dec2.144140.1@lure.latrobe.edu.au> Sender: news@emba.uvm.edu Followup-To: bionet.molbio.methds-reagnts,bionet.cellbiol,bionet.plants Organization: University of Vermont -- Division of EMBA Computer Facility Lines: 15 Xref: biosci bionet.molbio.methds-reagnts:9582 bionet.cellbiol:193 bionet.plants:2149 X-Newsreader: TIN [version 1.2 PL1] botbml@lure.latrobe.edu.au wrote: : Could someone please contact Jane Lancaster and ask her to reply to her e-mail. : Thanks in advance. You're welcome! I called all the Jane Lancasters' in Vermont (there were only two of them) and they both had no idea they even had an e-mail account. Perhaps they don't... -- ******************************************************************** * Brian Foley * If we knew what we were doing * * Molecular Genetics Dept. * it wouldn't be called research * * University of Vermont * * From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!evbhort.uoguelph.ca!JGERRATH From: JGERRATH@evbhort.uoguelph.ca ("Jean Gerrath") Newsgroups: bionet.plants Subject: Lilaea seeds Message-ID: Date: 2 Dec 93 15:26:57 GMT Sender: daemon@net.bio.net Distribution: bionet Organization: Environ. Biology & Horticulture Lines: 16 I am making a request for a student on whose Advisory Committee I am sitting. She is studying floral and inflorescence development in the Alismatidae, in order to examine some of the theories of floral development that have been postulated as the result of recent molecular biology work. She is having difficulty obtaining seeds of Lilaea scilloides, one of the plants she wants to study. This is an emergent plant that grows in vernal pools along the Pacific coast of North and South America, from California to Chile. If anyone knows of a seed source for this species, would they please contact me at: jgerrath@evbhort.uoguelph.ca Thankyou Dr. Jean M. Gerrath, Department of Horticultural Science University of Guelph, Guelph, ON N1G 2W1 From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!howland.reston.ans.net!darwin.sura.net!news-feed-2.peachnet.edu!concert!sol.cms.uncwil.edu!sol.cms.uncwil.edu!not-for-mail From: faldor@sol.cms.uncwil.edu Newsgroups: bionet.plants Subject: Amino-acid mapping of common plants. Message-ID: <2dleuc$oe4@sol.cms.uncwil.edu> Date: 2 Dec 93 19:16:28 GMT Organization: University of North Carolina @ Wilmington Lines: 20 NNTP-Posting-Host: sol.cms.uncwil.edu I was wondering where I could get a listing of the amino-acid sequencing for common garden/household plants. The type needed for genetic splicing. An ftp-site would be great. thanks, Faldor .---------.---------------------------------------------------------. | (__) | | | w \@@/ | Daemons do it with pointers. }:-) | | `/v/-e | VHDL, or die scribbling! | | _/ \_ | | ._________._________________________________________________________. | University of North Carolina at Wilmington | | faldor@seq.cms.uncwil.edu faldor@sol.cms.uncwil.edu | |-------------------------------------------------------------------| | Alien Invasion Division : Integration/Substitution Group. (ISG) | .___________________________________________________________________. From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!spool.mu.edu!caen!malgudi.oar.net!news.ysu.edu!psuvm!imeg From: IMEG@psuvm.psu.edu Newsgroups: bionet.plants Subject: MEGA: Mol. Evol. Gen. Analysis. Info Message-ID: <93336.092445IMEG@psuvm.psu.edu> Date: 2 Dec 93 14:24:45 GMT Expires: 31 Jan 94 00:00:00 GMT Organization: Penn State University Lines: 286 This document contains many pages, including 1. Cover letter 2. Order form (We no longer accept purchase orders) 3. Hardware and software requirements 4. Program upgrade policy 5. Functions in MEGA MEGA version 1.0, 1.01 September, 1993 To whom it may concern: A computer software called MEGA: Molecular Evolutionary Genetics Analysis has been developed. This software is for facilitating statistical studies of molecular evolution by using IBM compatible personal computers. It contains various methods of estimating evolutionary distances and three different methods of phylogenetic inference (UPGMA, neighbor-joining, and maximum parsimony) with either a standard-error test or a bootstrap test of topological differences. For the maximum parsimony method, new algorithms of the branch-and-bound and heuristic searches are implemented. MEGA also computes basic statistical quantities such as nucleotide and amino acid frequencies, transition/transversion bias, codon usage frequencies, number of variable sites, etc. In addition, advance on- screen sequence data and phylogenetic tree editors are included. Integrated and interactive designs, on-line context-sensitive helps, text-file editor, and other unique features make it easy to use MEGA. MEGA version 1.0 is distributed with a nominal fee to defray the cost of producing the user manual (140 pp.) and diskettes, and the mailing and handling expenses (see enclosed order form). However, for anyone who is unable to pay the fee for some reason, it will be provided free of charge after receiving a letter explaining the circumstances. MEGA will not be sent by electronic-mail because the accompanying manual cannot be included in this case. Technical questions and other inquires about MEGA should be directed to the senior author of the software. Please include your electronic-mail address, if any. Sincerely yours, Sudhir Kumar Koichiro Tamura Masatoshi Nei Telephone: (814) 863-7334 FAX: (814) 863-7336 E-mail: imeg@psuvm, imeg@psuvm.psu.edu MEGA ORDER FORM Cost* ----------------------------------------------------------------- Program No Charge User manual, diskette, shipping, and handling $ 15.00 For shipment outside North America add $10.00 _________ (We will use first class airmail) TOTAL _________ ----------------------------------------------------------------- * This cost can be waived if the circumstances for the inability to pay (e.g., lack of hard currencies in some countries) are explained. Diskette type desired: Please specify: [ ] 3.5" Diskette (1.44MB) [ ] DOS Version ...... [ ] 5.25" Diskette (1.2MB) [ ] Computer system... [ ] other (specify).... [ ] Do you use windows? Yes/No All orders must be prepaid in U.S. dollars. Return this form together with a check or money order payable to Penn State University at the following address. Purchase orders will not be accepted. Joyce White Institute of Molecular Evolutionary Genetics The Pennsylvania State University 328 Mueller Laboratory University Park, PA 16802 USA Telephone: (814)-863-7334 Fax: (814)-863-7336 E-mail: IMEG@PSUVM.PSU.edu, IMEG@PSUVM About the user: Name: ____________________________________________ Address: ____________________________________________ ____________________________________________ ____________________________________________ Telephone: _____________________ Fax: _________________ E-Mail: ____________________________________________ --------Hardware and Software------ IBM and IBM-compatible PCs, XTs, ATs, etc. Color/monochrome monitors. 640KB RAM memory. DOS version 3.3 or later. Hard disk with 2MB free. No extended or expanded memory required. No graphics adapters required. No math-chip required. Supports the keyboard as well as the mouse (not essential). ------- Program upgrade policy ---- Since MEGA is in its first version, there may be many software bugs in the program. We are considering to provide registered users a version where bugs reported in version 1.0 and 1.01 will be fixed. As you may notice from the order form, we are just trying to recover the cost of distributing MEGA only. So we are not in a position to provide many upgrades. In any case, we do not plan to include new methods in MEGA soon (for at least one year), and therefore the questions about upgrades may not be so pressing at this moment. -------Functions in MEGA------- Input Input data: DNA sequences RNA sequences Amino acid sequences Distance matrices Input formats: Interleaved sequences Non-interleaved sequences Upper-triangular distance matrix Lower-triangular distance matrix Choice of: Alignment gap symbol Missing-information site symbol Identical site symbol In-memory data editing features Selection: Desired OTUs Domains of sequences Individual sites and codons Codon positions Exclude/include missing information sites Exclude/include alignment gap sites Edit OTU labels Restore OTU labels Sequence data presentation Highlight: Variable sites Parsimony-informative sites Two-fold redundant sites Four-fold redundant sites Translate: Translation of nucleotide sequences into amino acid sequences Output: Formats: MEGA PAUP PHYLIP Publication Data subsets: Only variable sites Only parsimony-informative sites Amino acid sequences translated Codon positions Sequence statistics: Nucleotide and amino acid frequencies Nucleotide pair frequencies in pairwise comparisons Insertion-deletion frequencies Codon usage frequencies Relative synonymous codon usage (RSCU) values Variable sites in overlapping segments Variable sites in nonoverlapping segments Distance estimation Nucleotide substitutions Quantities: Number of nucleotide differences Nucleotide substitutions Transitional substitutions Transversional substitutions Transition/transversion ratio Distance measures: p-distance Jukes-Cantor distance Kimura 2-parameter distance Tajima-Nei distance Tamura distance Tamura-Nei distance Gamma distances Jukes-Cantor model Kimura 2-parameter model Tamura-Nei model Synonymous-nonsynonymous substitutions Genetic code tables: "Universal" Mammalian mitochondrial Drosophila mitochondrial Yeast mitochondrial Computation: Synonymous substitutions Nonsynonymous substitutions Average distances for all pairwise comparisons and standard errors Amino acid substitutions Distance measurers: Number of amino acid differences p-distance Poisson-correction distance Gamma distance Distance output: Control on: Page size Precision for distance output Distance q standard error formats Tree building and test Methods: Neighbor-joining (NJ) UPGMA Maximum parsimony (MP): Branch-and-bound search Heuristic search Statistical Tests: Bootstrap test: Neighbor-joining UPGMA Branch length test: Neighbor-joining Phylogeny editing: Tree re-rooting Swapping and flipping branches Consensus tree Condensed tree Phylogeny printing: Various printers Multiple page printouts Choice of fonts Choice of orientation Choice of page size Tree preview General functions File browsing File editing Exiting to DOS temporarily Context-sensitive Helps Error messages From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!europa.eng.gtefsd.com!emory!nigel.msen.com!yale.edu!xlink.net!math.fu-berlin.de!zib-berlin.de!uni-paderborn.de!urmel.informatik.rwth-aachen.de!gmd.de!dearn!barilvm!kineret.huji.ac.il!ramon.bgu.ac.il!mlewin From: mlewin@bgumail.bgu.ac.il (Michel Lewinger ) Newsgroups: bionet.plants Subject: Plant Salinity Message-ID: <1993Dec1.132227.12305@ramon.bgu.ac.il> Date: 1 Dec 93 13:22:27 GMT Sender: usenet@ramon.bgu.ac.il (The Ever-So-Great Usenet) Organization: Ben Gurion University, Beer Sheva, Israel Lines: 13 X-Newsreader: TIN [version 1.2 PL0] Hi. I'm new to the bionet.plants group, So I'd like to knwo which kind of people are around, and if someone also works with salinity effects on plants TIA Michel Lewinger /| mlewin@bgumail.bgu.ac.il / | BEERSHEVA-ISRAEL / *| \ | \_| --------------------------------------------------------- From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!Viroid.AgMarley.Arizona.EDU!xiong From: xiong@biosci.arizona.edu (Zhongguo Xiong, Ph.D) Newsgroups: bionet.plants Subject: HELP: Common plant protease ??? Summary: looking for common plant proteases in cytoplasm Keywords: transgenic plant, plant protease, peptide Message-ID: Date: 2 Dec 93 08:55:48 GMT Organization: University of Arizona Lines: 16 NNTP-Posting-Host: viroid.agmarley.arizona.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Dear netters: I am looking for some informations on the common proteases in plant cytoplasm. The project I am working on involves expressing small peptides in transgenic plants. Ideally, I would like to use concatmers of the peptides joined by spacers of a protease cleavage site, which can be recognized by a common and abundant protease existing in the cytoplasm. The multiple copies of the peptide would then be released from a single polypeptide by the protease. I realize there are many protease in the vacuoles, but am not familiar with any in the cytoplasm. Are trypsin and chymotrypsin good candidates? I will welcome any comments and suggestions. Sincerely, Zhongguo Xiong zxiong@arizvm1.ccit.arizona.edu From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!munnari.oz.au!ariel.ucs.unimelb.EDU.AU!ucsvc.ucs.unimelb.edu.au!lugb!lure.latrobe.edu.au!botbml From: botbml@lure.latrobe.edu.au Newsgroups: bionet.molbio.methds-reagnts,bionet.cellbiol,bionet.plants Subject: JANE LANCASTER Message-ID: <1993Dec2.144140.1@lure.latrobe.edu.au> Date: 2 Dec 93 04:41:40 GMT Sender: news@lugb.latrobe.edu.au (USENET News System) Organization: VAX Cluster, Computer Centre, La Trobe University Lines: 3 Xref: biosci bionet.molbio.methds-reagnts:9568 bionet.cellbiol:190 bionet.plants:2143 Could someone please contact Jane Lancaster and ask her to reply to her e-mail. Thanks in advance. From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!newsrelay.iastate.edu!news.iastate.edu!vincent1.iastate.edu!adwright From: adwright@iastate.edu Newsgroups: bionet.plants Subject: Re: Miracloth Message-ID: Date: 26 Nov 93 15:43:41 GMT References: <01H5QFK94FMQ0036M0@GW.AGR.CA> <1993Nov26.111517.4701@rhrk.uni-kl.de> Sender: news@news.iastate.edu (USENET News System) Distribution: bionet Organization: Iowa State University, Ames IA Lines: 6 I believe miracloth can also be obtained from Chicopee manufacturing co. A collegue told me that "parachute silk" is just as good. -- From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!uunet!biosci!CROP.UOGUELPH.CA!TMICHAEL From: TMICHAEL@CROP.UOGUELPH.CA ("Tom Michaels") Newsgroups: bionet.plants Subject: Re: USDA catalog? Message-ID: <2E1A48C7BAE@csnet.nw.uoguelph.ca> Date: 25 Nov 93 12:54:32 GMT Sender: daemon@net.bio.net Distribution: bionet Organization: Crop Science, The Univ. of Guelph Lines: 27 I suggest that you point your gopher server or anonymous ftp to sol.ars-grin.gov Files at that site give an introduction to the National Plant Germplasm System and also provide the opportunity to download pcGRIN, a very useful database of USDA collections for specific plant species. > I recently saw a plea for some sort of USDA catalog/seed bank by a > person from Quebec, but failed to see the response. Information of > this nature would greatly be appreciated, as it would aid progress on > my (undergrad) thesis. My address is: > jzanzot@reed.edu > thank you > >T. E. (Tom) Michaels Department of Crop Science University of Guelph Guelph, Ontario N1G 2W1 Canada Voice: 519-824-4120 ext. 3392 Fax: 519-763-8933 EMAIL: TMICHAEL@CROP.UOGUELPH.CA From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!xlink.net!rz.uni-karlsruhe.de!stepsun.uni-kl.de!sun.rhrk.uni-kl.de!schumann From: schumann@rhrk.uni-kl.de (Klaus Schumann [Biologie]) Newsgroups: bionet.plants Subject: Re: Miracloth Message-ID: <1993Nov26.111517.4701@rhrk.uni-kl.de> Date: 26 Nov 93 11:15:17 GMT References: <01H5QFK94FMQ0036M0@GW.AGR.CA> Distribution: bionet Organization: University of Kaiserslautern, Germany Lines: 21 REELEDER@ONRSDE.AGR.CA writes: Miracloth can also be obtained from Calbiochem, their phone number (toll free) for your country (ca = Canada?) is (800) 567-8098. The product no. is: 475855. One roll is 18 in. x 50 ft and the price (1992) in german Marks is: 133.- Kope this helps, Klaus Schumann schumann@rhrk.uni-kl.de >Hello, >Does anyone have information (address, phone number) on Behring Diagnostics >(La Jolla CA ??). They manufacture or supply a product called Miracloth >which we need for some experimental procedures. Thanks. >R. Reeleder >reeleder@onrsde.agr.ca From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!swrinde!sgiblab!munnari.oz.au!sol.ccs.deakin.edu.au!bbs.ausom.oz.au!bbs.ausom.oz.au!not-for-mail From: klb@bbs.ausom.oz.au (Kevin Blaze) Newsgroups: bionet.plants Subject: Re: How to start a bio.net algae newsgroup Message-ID: <2d3o7u$cct@ausom.ausom.oz.au> Date: 26 Nov 93 02:04:46 GMT References: <2cf371f9._indotec@indotec.redid.org.do> Reply-To: klb@ausom.oz.au Organization: AUSOM - The Apple Users Society of Melbourne Lines: 3 NNTP-Posting-Host: bbs.ausom.oz.au Dan, There's a bunch of Australians down here who'd be interested in the algal newsgroup. [BWe're always finding strange things to talk about. G.Kraft & K. Blaze, School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!europa.eng.gtefsd.com!uunet!biosci!SASK.USASK.CA!LEES From: LEES@SASK.USASK.CA Newsgroups: bionet.plants Subject: plant transposon required Message-ID: <01H5QIM6929K8WWD9F@SKYCAT.USask.CA> Date: 25 Nov 93 20:51:36 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 19 Hello, I've been looking for sources to get plant transposon vectors for gene-tagging experiment. Any of possible Tns such as Ac, Tam3, Ds mediated with GUS or other markers for this purpose. Is there any company selling these vectors? Any information regarding this matter will be greatly appreciated. Thanks, Sun Lee National Research Council Saskatoon, SK Canada S7N 0W9 e-mail: lees@skyfox.usask.ca fax: 306/975-4839 From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!olivea!biosci!ONRSDE.AGR.CA!REELEDER From: REELEDER@ONRSDE.AGR.CA Newsgroups: bionet.plants Subject: Miracloth Message-ID: <01H5QFK94FMQ0036M0@GW.AGR.CA> Date: 25 Nov 93 18:25:22 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 9 Hello, Does anyone have information (address, phone number) on Behring Diagnostics (La Jolla CA ??). They manufacture or supply a product called Miracloth which we need for some experimental procedures. Thanks. R. Reeleder reeleder@onrsde.agr.ca From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!umn.edu!msus1.msus.edu!TIGGER.STCLOUD.MSUS.EDU!J00030 From: j00030@TIGGER.STCLOUD.MSUS.EDU Newsgroups: bionet.plants Subject: Onion Chromosomes Message-ID: <1993Dec2.142019.6956@msus1.msus.edu> Date: 2 Dec 93 20:20:18 GMT Reply-To: j00030@TIGGER.STCLOUD.MSUS.EDU Organization: ST. CLOUD STATE UNIVERSITY, ST. CLOUD, MN Lines: 6 Nntp-Posting-Host: tigger.stcloud.msus.edu I am a graduate assistant in biology, and we need to know the number of chromosomes in the onion. We cannot find it anywhere, and know one here knows tha answer. It is for a general biology lab. From last quarters data I would have to say the number is 10. Can anyone confirm this? Thank you in advance. KR Ziarkowski St. Cloud State University St. Cloud, MN From owner-plants@net.bio.net Wed Dec 01 22:00:00 1993 Path: biosci!news.cs.umb.edu!hsdndev!rutgers!concert!samba.oit.unc.edu!sunSITE!london From: london@sunSITE.unc.edu (Larry London) Newsgroups: alt.sustainable.agriculture,bionet.plants,alt.agriculture.misc Subject: Sci.agriculture CFV Message-ID: <2dl0ms$7cv@samba.oit.unc.edu> Date: 2 Dec 93 15:13:32 GMT Organization: University of North Carolina, Chapel Hill Lines: 99 Xref: biosci alt.sustainable.agriculture:1889 bionet.plants:2135 alt.agriculture.misc:502 NNTP-Posting-Host: calypso.oit.unc.edu ---------- Forwarded message ---------- Date: Mon, 29 Nov 93 13:30:11 EST From: L. Todd Masco To: sustag@beta.tricity.wsu.edu Subject: CFV: sci.agriculture FIRST CALL FOR VOTES (of 2) This is the first of two Call For Votes (CFV) regarding the proposed creation of a new newsgroup. Please read the proposal before voting. Detailed instructions for voting are given below. Unmoderated group sci.agriculture Newsgroups line: sci.agriculture Farming, agriculture and related topics All votes must be received by 23:59:59 UTC, 15 December 1993. This vote is being conducted by a neutral third party. All questions regarding voting and procedure or reports of problems should be mailed to "L. Todd Masco" . All votes should be mailed to vote@clinton.com All questions regarding the proposed new group should be mailed to the proposer, David Bromage . This CFV is being posted to the following newsgroups: news.announce.newgroups news.groups alt.sustainable.agriculture bionet.agroforestry bionet.general sci.environment sci.misc talk.environment And once the CFV has appeared, it will also be mailed to the mailing list: sustag@beta.tricity.wsu.edu CHARTER This newsgroup is intended for the discussion of any topics related to farming and agriculture. Topics for discussion include, but are not limited to: - Land management - Water management - Soils - Erosion (prevention of) - Forestry - Hydroponics - Aeroponics - Aquaculture - Permaculture - Economic aspects - Advice and assistance including personal experience - Announcement of forthcoming courses and conferences - Discussion and criticism of literature VOTING INSTRUCTIONS You should send MAIL (posts to any newsgroup or mail to any other address are invalid) to vote@clinton.com (just replying by MAIL to this message should work). Your mail message's body should contain one and only one of the following exact statements: I vote YES on sci.agriculture I vote NO on sci.agriculture I vote ABSTAIN on sci.agriculture I vote CANCEL on sci.agriculture Anything else may be rejected by the automatic vote counting program. You may ABSTAIN in place of YES or NO - abstain votes do not affect the vote outcome; you may also change your vote later by voting again. Within a few days, the votetaker will respond to received ballots with mail acknowledging your vote. Addresses and votes of all voters will be published in the vote results. If you wish to remove your vote and all indication that you have voted from the final list, send a vote with CANCEL in the place of YES/NO/ABSTAIN. Standard Guidelines for voting apply. One vote per person, no more than one vote per account. 100 more YES votes than NO votes and twice as many YES votes as NO votes are the requirements for group creation. Votes must be tracable to a human: votes from system accounts such as (but not limited to) "news" or "root" will be marked invalid. -- L. Todd Masco This vote is being handled by the System Administrator/Programmer, USENET Volunteer Votetakers. From owner-plants@net.bio.net Thu Dec 02 22:00:00 1993 Path: biosci!PHIBRED.COM!murrayian From: murrayian@PHIBRED.COM (IAN MURRAY) Newsgroups: bionet.plants Subject: Grinding plant tissue Message-ID: <9312022126.AA11507@phibred.phibred.com> Date: 2 Dec 93 21:26:42 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 20 Hello and good day, I am looking for disposable/ resuable pestles to fit into the wells of a 96 well microtitre plate or a 1 ml tube, the ones that I have are supplied by Bio Rad. I have tried Kontes, Bel Art and a few others. I was wondering if anyone could shed some shed some light on the matter, OR give me some references as to which pestles they use. Your help is much appreciated. Murrayian@phibred.com Disclaimer: I don't have any financial interest in the companies named above and am not promoting the use of their products *smile* From owner-plants@net.bio.net Thu Dec 02 22:00:00 1993 Path: biosci!lhc!darwin.sura.net!udel!news.sprintlink.net!uunet!europa.eng.gtefsd.com!library.ucla.edu!agate!doc.ic.ac.uk!warwick!unicorn.nott.ac.uk!ppbnwb.nottingham.ac.uk!plznb From: plznb@vax.nott.ac.uk (Nigel Blackhall) Newsgroups: bionet.plants Subject: Re: tomato Message-ID: Date: 2 Dec 93 13:45:21 GMT References: <2dc7jf$mvo@mserv1.dl.ac.uk> Distribution: bionet Organization: Dept Life Science Lines: 20 NNTP-Posting-Host: ppbnwb.nottingham.ac.uk In article <2dc7jf$mvo@mserv1.dl.ac.uk> "M. Emre Oktem" writes: >From: "M. Emre Oktem" >Subject: tomato >Date: 29 Nov 1993 07:15:59 -0000 > Dear friends, > I am a fourth year biology student in M.E.Tech,Turkey. > I am interested and performing experiments on tomato. > If anyone interested in tomato transformation or transformation > via Agrobacterium please do write me. > Dear Emre Oktem, I noticed your posting today, 2nd December, 1993. Although I work on somatic hybridisation of rice, I know that other members of our research group work on tomato transformation via agrobacteria. I suggest you contact our Professor: Professor E.C. Cocking, Plant Genetic Manipulation Group, Dept. Life Science, Nottingham University, University Park, Nottingham, NG7 2RD, UK. Best wishes, Nigel Blackhall, Plant Genetic Manipulation Group, etc.... From owner-plants@net.bio.net Thu Dec 02 22:00:00 1993 Path: biosci!kristoff From: kristoff@net.bio.net (David Kristofferson) Newsgroups: bionet.plants Subject: IMPORTANT!!! - BIOSCI at net.bio.net off-line this ENTIRE WEEKEND!! Message-ID: Date: 3 Dec 93 00:33:36 GMT Organization: BIOSCI International Newsgroups for Biology Lines: 26 We are aware of continuing problems with duplicated messages and are taking the BIOSCI system off-line from 5 PM Friday, 6 DEC, until Monday AM. The message duplication is caused by a relatively infrequent but recurring file corruption problem that we have been experiencing with our news message tracking database. We will be doing a news system software upgrade over the weekend. All BIOSCI services including e-mail, news, WAIS, Gopher, FTP archive access, etc. will be inaccessible over the weekend, so here is your chance to take a short break from e-mail overload 8-). I am going to post this note to several of the more popular groups to ensure that as many readers as possible see it. My apologies if you subscribe to several of these groups by e-mail - you will get one copy for each group that you receive. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-plants@net.bio.net Thu Dec 02 22:00:00 1993 Path: biosci!news.cs.umb.edu!hsdndev!yale!yale.edu!spool.mu.edu!agate!overload.lbl.gov!bks From: bks@s27w007.pswfs.gov (Bradley K. Sherman) Newsgroups: bionet.plants Subject: Re: dendrome project Message-ID: <2dlul0$1qe@overload.lbl.gov> Date: 2 Dec 93 23:44:32 GMT References: <2dhvd2$u9@mserv1.dl.ac.uk> Distribution: bionet Organization: Dendrome, A Genome Database for Forest Trees Lines: 28 NNTP-Posting-Host: s27w007.pswfs.gov In article <2dhvd2$u9@mserv1.dl.ac.uk> H.F.J.Bligh@vme.ccc.nottingham.ac.uk writes: >I've seen several postings over the past few months on the dendrome project > ... more details if anyone out there could send me them ... The Dendrome project is part of the Plant Genome Database project of the National Agricultural Library (USDA ARS). Our focus is the molecular biology and genome mapping of forest trees. We have laboratories in Albany CA (US Forest Service Pacific Southwest Research Station) and in Placerville CA (Institute of Forest Genetics). You can find out more via the gopher server at s27w007.pswfs.gov port 70 or the NAL WWW http server whose URL is http://probe.nalusda.gov:8000/index.html I can also send out literature by mail if given an address. --bks -- Bradley K. Sherman P.O. Box 245 Computer Scientist Berkeley, CA, 94701 Dendrome Project 510-559-6437 FAX: 510-559-6440 Institute of Forest Genetics Internet: bks@s27w007.pswfs.gov From owner-plants@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!daresbury!bioftp.unibas.ch!rc1!ub4b!EU.net!howland.reston.ans.net!spool.mu.edu!nigel.msen.com!caen!msuinfo!thomashaw-at.css.msu.edu From: bloksber@pilot.msu.edu (Leonard N. Bloksberg) Newsgroups: bionet.plants Subject: Auxin/toxic? Message-ID: <19931203085645.bloksber@thomashaw-at.css.msu.edu> Date: 3 Dec 93 12:56:00 GMT Organization: Michigan State U., Crop & Soils Dept. Lines: 21 NNTP-Posting-Host: thomashaw-at.css.msu.edu X-Newsreader: FTPNuz (DOS) v1.0 . Quick Lazy mans question... . . Are plant auxins (IAA, NAA, 24D) carcinogenic? . When I was an undergrad, we were taught that in the concentrations and purity that we use in the lab, they are. Recently a post doc in our lab told our technician not to worry, they have no toxic or carcinogenic properties. I am afraid that he may be leaving himself and the lab open to criminal charges under the current employee right to know laws with his polyanna attitude, but I can't find my old reference for this little trivia factoid. Can someone point me to a med reference, or some such that might help? Thanks. . . Leonard N. Bloksberg . Dept. of Crop and Soil Science . Michigan State University . Bloksber@pilot.msu.edu . . . From owner-plants@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!daresbury!mrccrc!news.dcs.warwick.ac.uk!warwick!uknet!pipex!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!msuinfo!thomashaw-at.css.msu.edu From: bloksber@pilot.msu.edu (Leonard N. Bloksberg) Newsgroups: bionet.plants Subject: Re: tomato Message-ID: <19931203103610.bloksber@thomashaw-at.css.msu.edu> Date: 3 Dec 93 14:36:00 GMT References: <2dc7jf$mvo@mserv1.dl.ac.uk> Organization: Michigan State U., Crop & Soils Dept. Lines: 36 NNTP-Posting-Host: thomashaw-at.css.msu.edu X-Newsreader: FTPNuz (DOS) v1.0 In Article <2dc7jf$mvo@mserv1.dl.ac.uk> ""M. Emre Oktem" " says: > Dear friends, > I am a fourth year biology student in M.E.Tech,Turkey. > I am interested and performing experiments on tomato. > If anyone interested in tomato transformation or transformation > via Agrobacterium please do write me. > > Yours sincerely, > Emre Oktem > . I have done tomato transformation. Try looking up some of the current lit for the most up to date protocols. Some warnings and advice that I can give. In the Horsch and Fraley paper, Science, 1985, they speak of transforming tomato. The "tomato" they refer to is a wild relative of tomato which was selected for easy regeneration in tissue culture, and has no similarity to the cultivated tomato with regards to conditions for tissue culture. Just the same, this paper is the basis of all current protocols, and is very good. Note that cultivars of tomato vary dramatically in their response to tissue culture. Find a protocol that works for your cultivar and stick with it. The pre-incubation of explants on a nurse culture used in some protocols makes a big difference in speed and efficiency of regeneration. The exact age of sterile grown seedlings is important (7 day old is better than 5 or 9) and do not even try to surface sterilize field grown material. The shock of sterilization combined with the shock of agro infection is lethal. I don't have my ref's with me, but I'm sure you can find more recent stuff than I have. I was involved in developing the first system. Good luck. . . Dr. Leonard N. Bloksberg . Bloksber@pilot.msu.edu . Dept. of Crop and Soil Science . Michigan State University . East Lansing, MI 48824 USA . . . From owner-plants@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!AGCS.CAS.PSU.EDU!Mark_Guiltinan From: Mark_Guiltinan@AGCS.CAS.PSU.EDU ("Mark Guiltinan") Newsgroups: bionet.plants Subject: HIGH TECH GREENHOUSES (RESPONSES) Message-ID: <9312031513.AA14375@net.bio.net> Date: 3 Dec 93 14:22:40 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 288 Many thanks to all of the wonderful people who responded to my question about the design of high tech-physiological greenhouses. This is really a fantastic system. Here are all of the responses I have received so far. If anyone has more information, please forward and I will repost a second summary later. Thanks again, Mark Guiltinan Penn State Biotechnology Institute mjg@psupen.psu.edu PS. I have no affiliation with any of the companies or consultants endorsed herein. MG =============================================================== Message for Mark Guiltinan From: yams@midway.uchicago.edu Date: Mon, Nov 29, 1993 10:58 AM Subject: High tech greenhouses To: mjg@psupen.psu.edu Mark, We have just gone through the process you are now starting. It will be about five years from the start of design to the final completion of our new greenhouse facility. The greenhouses are under construction now, and we expect to move in during spring of '94. There are lots of design considerations, names of appropriate greenhouse manufacturers, and names other equipment manufacturers I could mention. But I think we found the most important consideration was to find a greenhouse consultant to help us with the planning and construction processes. We used a Canadian company, Agritech nove, located at 5 rue Morin, St. Anselme, Quebec, Canada, G0R 2N0. Their phone number is 418/885-9595 and their fax is 418/885-4957. Richard Denis is the president of the company, and he has been intimately involved with our project. Since we are not yet moved into our new facility, I cannot offer advice as to what has worked well and what we would would change. I can think of two new university greenhouse facilities which are already in operation, and you may want to contact people there. They are: 1. James D. Kramer Dept. of Plant Biology University of Illinois 505 S. Goodwin Ave. Urbana, IL 61801 phone: (217) 333- 3058 email: j-kramer@uiuc.edu 2. Joan Leonard Department of Plant Biology The Ohio State University Columbus, OH 43210 phone: (614) 292-7904 If there is any further info I can send you, let me know. I do think finding a good consultant is the best way to start, particularly if your university does not have its own ag. engineering dept. to use a resource when designing the new greenhouse. Susan Yamins Greenhouse Manager The University of Chicago Dept. of Ecology & Evolution ============================================================ Message for Mark Guiltinan From: PTAG2::KRUEGERR Date: Mon, Nov 29, 1993 8:51 AM Subject: RE: High Tech Greenhouse Design To: Mark Guiltinan While I was part of Dekalb Plant Genetics, we designed a new greenhouse that would be used for physiological, transgenic and insect studies. The houses were finished in 1992. Dekalb Plant Genetics, Mystic, CT would be a good contact for feedback. Try calling Peter Orr, 203-572-5231. Roger Krueger ============================================================ Message for Mark Guiltinan From: Fred Lehle Date: Sat, Nov 27, 1993 3:49 PM Subject: Re: +Postage Due+High Tech Greenhouse Design To: \"Mark Guiltinan\" Nov 27, 1993 Dear Mark, I would be interested in whatever responses you get back concerning greenhouse construction experiences. Could you post a summary of your responses to me or the newsgroup as a whole? Thanks, Fred Lehle LEHLE SEEDS >internet:75070.2510@compuserve.com ============================================================ Message for Mark Guiltinan From: peter@carrot.mcb.uconn.edu Date: Fri, Nov 26, 1993 3:55 PM Subject: gequest to forward greenhouse mail To: mjg@psupen.psu.edu We are currently planning the relocation/re-building of the research green houses at UCONN. Could you please forward any interesting responses that you receiver to my e-mail address? Thanks a lot Peter Gogarten (Peter@carrot.mcb.uconn.edu) In article <9311242043.AA06830@net.bio.net> Mark_Guiltinan@AGCS.CAS.PSU.EDU ("Mark Guiltinan") writes: > > We are in the process of planning the construction of a new "state of the art" greenhouse for physiological studies. We would like to have the input (both positive and negative) of others who have been through this before. Who out there has "state of the art" greenhouses? What are the variable controlled and what are the best ways to control them. What are the general considerations you have made such as section size, basic framework plan etc.? What are the best companies for this sort of construction. We would greatly appreciate your time and effort in answering this posting, even if it is just a lead to a person or company you know who could help us. > >Thanks in advance, > >Mark Guiltinan >Penn State Biotechnology Institute >mjg@psupen.psu.edu ============================================================ Message for Mark Guiltinan From: KINGJ@sask.usask.ca Date: Thu, Nov 25, 1993 1:05 PM Subject: Greenhouse construction To: mjg@psupen.psu.edu Dr. Guiltinan: You might want to contact Mike Dixon in the Department of Horticultural Sciences, University of Guelph, Guelph, ON. N1G 2W1, Canada, Tel.(519)824-4120 ext. 2555. A very few years ago, the University of Guelph installed extensive new greenhouse facilities for plant biotechnological and physiological research. Mike spearheaded that initiative Regards, John King, University of Saskatchewan. ============================================================ Message for Mark Guiltinan From: BTNYDWM@MVS.UCC.OKSTATE.EDU Date: Thu, Nov 25, 1993 12:35 PM Subject: Greenhouse Delight To: mjg@psupen.psu.edu Mark: Check with Richard Dixon, Head of Plant Biology, S.R. Noble Foundation, Ardmore, OK. The phone # is: 405-223-5810. They just recently completed a beautiful greenhouse and could certainly give you some advice. Regards - David Meinke ============================================================ Message for Mark Guiltinan From: zrsung@nature.berkeley.edu Date: Wed, Nov 24, 1993 5:31 PM Subject: Re: High Tech Greenhouse Design To: Mark Guiltinan Dear Mark, We had a new greenhouse built for our Department 3 years ago. It seems O.K..I am not clear on the specifics, but you might contact Bob Fischer (Tel. no. 510 642 1314) in our Department who was on the greenhouse committee, or our greenhouse manager, Barbara Rotz (510 642 8188 or 642 2047). There are about 8-10 sections with temperature and daylength control. It is state of the art, because the curtains and temperatures are controlled by the computer. In addition, there is an office where the control panels are, a head house for soil and transplantation, a teaching lab, several small research rooms with benches, and a bathroom facility fitted with showers. Renee Sung ============================================================ Message for Mark Guiltinan From: daniel@jeeves.ucsd.edu Date: Wed, Nov 24, 1993 4:51 PM Subject: Re: High Tech Greenhouse Design To: Mark Guiltinan Dear Mark: My suggestion would be to contact Dr. Howard Rawson at CSIRO Plant Industry in Canberra Australia. He knows more about environmental control than anyone I have ever met. Cheers, Daniel ============================================================ Message for Mark Guiltinan From: KIRKCAM@WVNVAXA.WVNET.EDU Date: Thu, Dec 2, 1993 10:35 AM Subject: Re: High Tech Greenhouse Design To: Mark Guiltinan Hello Mark. I too need to completely renovate a greenhouse to be high tech for transgenics, etc.. I would like to know of information received in reply to your arabnet posting, perhaps you could post it or send it to me? Many thanks, Kirk Cammarata Salem_teikyo University Biotechnology Program kirkcam@wvnms.wvnet.edu ============================================================ Message for Mark Guiltinan From: Petercsak, Michael R Date: Thu, Dec 2, 1993 4:41 PM Subject: Greenhouse Construction Advise To: Mark Guiltinan I supervise a small research greenhouse for the Waksman Institute here at Rutgers. I would like to offer to you the names of individuals who have been helpful to me in the past. When you say "high tech" greenhouse I think you need to clarify what specific features are most important to your use. These may be very different for a commercial grower vs. a university research department. Joe Florentine is the supervisor of the newest greenhouse facility built for Cook College at Rutgers. He can be reached by phone at (908) 932-9034. Eric Melton is the greenhouse supervisor for American Cyanamid. They have finished (?) the planning stage and are about to begin construction of a new facility. Eric can be reached at American Cyanamid, Agricultural Center, P.O. Box 400, Princeton, New Jersey 08543-0400 or call (609) 799-0400. Rutgers sponsers a two day class called "Design of Greenhouse Systems and Environmental Control". The next session is scheduled for 1/10 - 1/11/1994. The fee is $235.00. The course coordinator is Professor William J. Roberts, he can be reached at the Department of Bioresource Engineering, Cook College, Rutgers University, P.O. Box 231, New Brunswick, New Jersey 08903-0231 or call (908) 932-9534. I hope these will be helpful resources. Good Luck. --Michael R. Petercsak From owner-plants@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!daresbury!bioftp.unibas.ch!embl-heidelberg.de!uni-heidelberg!rz.uni-karlsruhe.de!stepsun.uni-kl.de!sun.rhrk.uni-kl.de!schumann From: schumann@rhrk.uni-kl.de (Klaus Schumann [Biologie]) Newsgroups: bionet.plants Subject: Re: Onion Chromosomes Message-ID: <1993Dec3.093633.1919@rhrk.uni-kl.de> Date: 3 Dec 93 09:36:33 GMT References: <1993Dec2.142019.6956@msus1.msus.edu> Organization: University of Kaiserslautern, Germany Lines: 18 j00030@TIGGER.STCLOUD.MSUS.EDU writes: The chromosome number of Allium cepa is 16 (2n). Within the genus there is some variation from 32 to 14. The reference is: Bennett, MD; Smith, JB: Nuclear DNA amounts in angiosperms. Philosophical Transactions of the Royal Society of London (B. Biological Sciences) 274: 227-274 (1976). This is not the primary reference, it is a list where the DNA content for many plant species are given in addition with the chromosome number, and I had it on hand :-). Hope this helps Klaus schumann@rhrk.uni-kl.de > I am a graduate assistant in biology, and we need to know the number of chromosomes in the onion. We cannot find it anywhere, and know one here knows tha answer. It is for a general biology lab. From last quarters data I would have to say the number is 10. Can anyone confirm this? > Thank you in advance. > KR Ziarkowski > St. Cloud State University > St. Cloud, MN From owner-plants@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!spool.mu.edu!uwm.edu!msuinfo!via-annex2-2.cl.msu.edu From: Bloksber@pilot.msu.edu (Leonard N. Bloksberg) Newsgroups: bionet.plants Subject: Re: Vitemines & Plant growth/science fair proj. Message-ID: <19931203213654.Bloksber@via-annex2-2.cl.msu.edu> Date: 4 Dec 93 02:36:00 GMT References: Organization: Michigan State University Lines: 29 NNTP-Posting-Host: via-annex2-2.cl.msu.edu X-Newsreader: FTPNuz (DOS) v1.0 In Article "RBOLT.300@POSTMAN.GSFC.NASA.GOV (Lightning Bolt)" says: > Does anyone know the effects of different vitemines on plant growth? A? C? > B? E? > > Dick. Step son doing science project on this. Presently on onions from > bulb! Not my idea!!! > . There is lots of info on the subject. A classic research paper to read on the subject is: Murashige, T. and Skoog, F., "A revised medium for rapid growth and bio-assay with tobacco tissue cultures" Physiologia Plantarum, 15, 473-497 (1962). This was the first report of a defined plant growth medium, and is on my list of classic papers to read for the historical and literary value, even if you aren't interested in the field. In addition, there is a short book (easy reading for a kid) which discusses the subject: Gamborg, O.L., and Wetter, L.R., "Plant tissue culture methods" National research council of Canada, Sasdatoon, Saskatchewan. 1975. I'm sure there is more, especially in the details of specific vitamin effects, but these are the ones I use. Happy parenting, and tell your son I said good luck. . . Dr. Leonard N. Bloksberg . Bloksber@pilot.msu.edu . . . . From owner-plants@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!spool.mu.edu!uwm.edu!msuinfo!via-annex2-2.cl.msu.edu From: Bloksber@pilot.msu.edu (Leonard N. Bloksberg) Newsgroups: bionet.plants Subject: Re: Vitemines & Plant growth/science fair proj. Message-ID: <19931203213654.Bloksber@via-annex2-2.cl.msu.edu> Date: 4 Dec 93 02:36:00 GMT References: Organization: Michigan State University Lines: 29 NNTP-Posting-Host: via-annex2-2.cl.msu.edu X-Newsreader: FTPNuz (DOS) v1.0 In Article "RBOLT.300@POSTMAN.GSFC.NASA.GOV (Lightning Bolt)" says: > Does anyone know the effects of different vitemines on plant growth? A? C? > B? E? > > Dick. Step son doing science project on this. Presently on onions from > bulb! Not my idea!!! > . There is lots of info on the subject. A classic research paper to read on the subject is: Murashige, T. and Skoog, F., "A revised medium for rapid growth and bio-assay with tobacco tissue cultures" Physiologia Plantarum, 15, 473-497 (1962). This was the first report of a defined plant growth medium, and is on my list of classic papers to read for the historical and literary value, even if you aren't interested in the field. In addition, there is a short book (easy reading for a kid) which discusses the subject: Gamborg, O.L., and Wetter, L.R., "Plant tissue culture methods" National research council of Canada, Sasdatoon, Saskatchewan. 1975. I'm sure there is more, especially in the details of specific vitamin effects, but these are the ones I use. Happy parenting, and tell your son I said good luck. . . Dr. Leonard N. Bloksberg . Bloksber@pilot.msu.edu . . . . From owner-plants@net.bio.net Fri Dec 03 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!spool.mu.edu!umn.edu!msus1.msus.edu!TIGGER.STCLOUD.MSUS.EDU!DEGROOTE From: degroote@TIGGER.STCLOUD.MSUS.EDU Newsgroups: bionet.plants Subject: high fructose corn syrup Message-ID: <1993Dec3.223735.6998@msus1.msus.edu> Date: 4 Dec 93 04:37:34 GMT Reply-To: degroote@TIGGER.STCLOUD.MSUS.EDU Organization: ST. CLOUD STATE UNIVERSITY, ST. CLOUD, MN Lines: 3 Nntp-Posting-Host: tigger.stcloud.msus.edu The ethnobotanist asked my today how they get the high-fructose in corn syrup these days. He indicated that the old karo was not all that sweet but now the corn syrup is used as a sweetener all over. Any info would be appreciated Davide From owner-plants@net.bio.net Sat Dec 04 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!uunet!munnari.oz.au!bunyip.cc.uq.oz.au!dpi-gw!qdpii!ianst From: ianst@qdpii.ind.dpi.qld.gov.au (Ian Staples) Newsgroups: bionet.plants Subject: Why no chromosome numbers? [Was: Onion Chromosomes] Message-ID: Date: 5 Dec 93 07:48:42 GMT References: <1993Dec2.142019.6956@msus1.msus.edu> <1993Dec3.093633.1919@rhrk.uni-kl.de> Organization: Queensland Dept of Primary Industries Lines: 38 >> I am a graduate assistant in biology, and we need to know the number of chromosomes in the onion. We cannot find it anywhere, and know one here knows tha answer. It is for a general biology lab. From last quarters data I would have to say the number is 10. Can anyone confirm this? [There seems to be a severe shortage of line endings at St. Cloud ;-) ] >> Thank you in advance. >> KR Ziarkowski >> St. Cloud State University >> St. Cloud, MN Does this mean the "chromosome bible" of my youth is out of print? :-( Namely: Darlington, C.D. and Wylie, A.P. (1955) _Chromosome Atlas of Flowering Plants._ (London: George Allen & Unwin Ltd). Pp. xix+519 Cheers, Ian S. P.S. Not having D&W at my side, I checked out several floras, dictionaries of flowering plants, manuals of cultivated plants, and a few plant breeding and cytology & cytogenetics texts - I hadn't realised how badly we are served when it comes to such a fundamental thing as chromosome number! In 9 such texts scanned, the best I could find was _Allium_ n=7,8,9. Nothing for onion _per se_ (_A. cepa_). Finally, in the 10th book (Winchester, A.M. 1958(!) - _Genetics_. 2nd ed. (Boston: Houghton Mifflin Co.) Pp. xiii+413) I found an off-hand reference in the caption to fig. 15-11 (concerning the effect of colchicine on chromosome doubling in root tips): "There are 28 rather than the normal 14 chromosomes ..." So I guess n=7 for _A. cepa_. I find it absurd that chromosome numbers are not quoted more commonly in floras, manuals, and similar compendia. Nor even in plant breeding, cytology, and similar texts where individual species are often used as examples and illustrations for various phenomena and techniques. -- Ian Staples E-mail : ianst@qdpii.ind.dpi.qld.gov.au c/- P.O. Box 1054 MAREEBA Phone : +61 (0)70 921 555 Home 924 847 Queensland Australia 4880 Fax : +61 (0)70 923 593 " " " From owner-plants@net.bio.net Sat Dec 04 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!howland.reston.ans.net!news.cac.psu.edu!psuvm!tlc112 From: TLC112@psuvm.psu.edu (The One and Only) Newsgroups: bionet.plants Subject: Plant cuttings. Message-ID: <93339.010207TLC112@psuvm.psu.edu> Date: 5 Dec 93 06:02:07 GMT Organization: Penn State University Lines: 10 Ok, simple stuff. I realize that I'm way out of league here, but.... Where, in relation to the node should one cut a house plant in order to root it? Also, has anyone had any experience with "crystal soil" jello looking potting mix? Is it worth a try? Thanks.... Terry From owner-plants@net.bio.net Sat Dec 04 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!howland.reston.ans.net!vixen.cso.uiuc.edu!newsrelay.iastate.edu!cobra.uni.edu!sunfish!charlie.usd.edu!TSCHRODE From: tschrode@charlie.usd.edu Newsgroups: bionet.plants Subject: Small Everlasting Antennaria Message-ID: Date: 4 Dec 93 23:35:16 GMT Sender: news@sunfish.usd.edu Reply-To: tschrode@charlie.usd.edu Organization: The University of South Dakota Computer Science Dept. Lines: 4 Nntp-Posting-Host: charlie I am a student at the U. of South Dakota, I have job doing work-study in my scool's green-house. Recently I propogated a small population of small ever- lasting from cuttings. I placed the cuttings in modified Hoagland's solution, the plants now have established roots and I want to transplant them into a soil mixture. I will prbably use a commercial potting soil, sphagnum and possiby ver miculite. However I am very open to any suggestions since I am but a lowly unde r-gradute and my knowledge is liomite d From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!uunet!caen!malgudi.oar.net!witch!tytownms!mbridges From: mbridges@tytownms.win.net (Michael H. Bridges) Newsgroups: bionet.plants Subject: Re: Plant cuttings. Message-ID: <164@tytownms.win.net> Date: 5 Dec 93 16:25:25 GMT References: <93339.010207TLC112@psuvm.psu.edu> Reply-To: mbridges@tytownms.win.net (Michael H. Bridges) Lines: 25 In article <93339.010207TLC112@psuvm.psu.edu>, The One and Only (TLC112@psuvm.psu.edu) writes: >Ok, simple stuff. I realize that I'm way out of league here, but.... >Where, in relation to the node should one cut a house plant in order to root >it? In my experience, it doesn't matter, though cutting slightly below a node, and making sure the node is at or below the soil surface when the cutting is stuck, is the recommended method. >Also, has anyone had any experience with "crystal soil" jello looking potting >mix? Is it worth a try? Not as a pure potting mix, but as an additive to a commercial mix, possibly. The stuff is really too expensive to justify its use for me (commercially), but you may find it useful on valuable plants that you sometimes neglect to water properly. Michael Bridges mbridges@tytownms.win.net Southern Perennials & Herbs Tylertown, Mississippi (Wholesale Nursery) (601)684-1769 From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!caen!malgudi.oar.net!witch!tytownms!mbridges From: mbridges@tytownms.win.net (Michael H. Bridges) Newsgroups: bionet.plants Subject: Re: Plant cuttings. Message-ID: <164@tytownms.win.net> Date: 5 Dec 93 16:25:25 GMT References: <93339.010207TLC112@psuvm.psu.edu> Reply-To: mbridges@tytownms.win.net (Michael H. Bridges) Lines: 25 In article <93339.010207TLC112@psuvm.psu.edu>, The One and Only (TLC112@psuvm.psu.edu) writes: >Ok, simple stuff. I realize that I'm way out of league here, but.... >Where, in relation to the node should one cut a house plant in order to root >it? In my experience, it doesn't matter, though cutting slightly below a node, and making sure the node is at or below the soil surface when the cutting is stuck, is the recommended method. >Also, has anyone had any experience with "crystal soil" jello looking potting >mix? Is it worth a try? Not as a pure potting mix, but as an additive to a commercial mix, possibly. The stuff is really too expensive to justify its use for me (commercially), but you may find it useful on valuable plants that you sometimes neglect to water properly. Michael Bridges mbridges@tytownms.win.net Southern Perennials & Herbs Tylertown, Mississippi (Wholesale Nursery) (601)684-1769 From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!news.cs.umb.edu!hsdndev!yale!yale.edu!spool.mu.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!newsrelay.iastate.edu!cobra.uni.edu!sunfish!charlie.usd.edu!TSCHRODE From: tschrode@charlie.usd.edu Newsgroups: bionet.plants Subject: Small Everlasting Antennaria Message-ID: Date: 4 Dec 93 23:35:16 GMT Sender: news@sunfish.usd.edu Reply-To: tschrode@charlie.usd.edu Organization: The University of South Dakota Computer Science Dept. Lines: 4 Nntp-Posting-Host: charlie I am a student at the U. of South Dakota, I have job doing work-study in my scool's green-house. Recently I propogated a small population of small ever- lasting from cuttings. I placed the cuttings in modified Hoagland's solution, the plants now have established roots and I want to transplant them into a soil mixture. I will prbably use a commercial potting soil, sphagnum and possiby ver miculite. However I am very open to any suggestions since I am but a lowly unde r-gradute and my knowledge is liomite d From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!spool.mu.edu!olivea!decwrl!decwrl!waikato!waikato.ac.nz!fri.cri.nz!cokera From: cokera@fri.cri.nz Newsgroups: bionet.plants Subject: Ph.D scholarship, NEW ZEALAND Message-ID: <1993Dec6.113359.768@fri.cri.nz> Date: 5 Dec 93 22:33:59 GMT Organization: New Zealand Forest Research Institute Lines: 82 PhD SCHOLARSHIP OFFERED IN NEW ZEALAND 1994 CYTOKININS -REGULATORS OF JUVENILITY AND MATURATION IN PINUS RADIATA ? Maturation as a developmental process is poorly understood despite the considerable significance age manipulation plays in the genetic improvement of radiata pine. Superior radiata pine cannot be identified until seven to eight years of age when maturation characteristics are expressed. Cuttings taken at maturity exhibit undesirable properties for propagation including a reduction in diameter growth and rooting ability. From evidence to date it appears that maturation occurs within the bud as a consequence of self-programming and a combination of inter- and intracellular signalling. Over a period of time, the bud acquires a certain individuality with "memory " of age persisting even after excision from the plant for vegetative propagation. This forms the basis of the mature micropropagation procedure developed at New Zealand Forest Research Institute and offers a novel system for manipulating and understanding age-related events. In this system , exogenous cytokinins cause a "rejuvenation " of mature P.radiata in vitro, at least initially. Cytokinins are known to be important regulators of growth and development . The morphological and physiological phenotype characteristic of a cytokinin effect, represents a suite of events over a period of time overlaid on a framework of biochemical and molecular events. The aim of this project will be to determine whether cytokinins are involved in the determination of the juvenile/mature phenotype and studies will be carried out to: - analyse endogenous cytokinins in mature and juvenile material by HPLC and radioimmunoassays - compare cytokinin metabolism in juvenile and mature tissues - manipulate the endogenous cytokinin complement through transformation studies to investigate in vivo expression of a cytokinin gene The project will be jointly supervised at New Zealand Forest Research Institute by Ms Astrid Coker and Ms Kathryn Horgan and at the Department of Plant Biology and Biotechnology , Massey University Palmerston North,NZ by Dr Paula Jameson. Enquiries and applications can be made to Dr Paula Jameson, Dept Plant Biology and Biotechnology, Massey University, Private Bag 11222, Palmerston North NZ. FAX: +64 6 350 5623 TELEPHONE: +64 6 356 9099, extension 7518. OR CokerA@tawa.fri.cri.nz Applications should include a copy of your academic record, a description of research experience and interests and names of two referees. Closing date : 18th January 1994 From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!EU.net!uunet!munnari.oz.au!newsroom.utas.edu.au!zoology49h38.zoo.utas.edu.au!user From: Mario.Salamon@zoo.utas.edu.au (Mario Salamon) Newsgroups: bionet.plants Subject: HELP: Looking for Japanese plant oil Message-ID: Date: 6 Dec 93 00:46:08 GMT Followup-To: bionet.plants Organization: University of Tasmania Lines: 20 NNTP-Posting-Host: zoology49h38.zoo.utas.edu.au Hello out there, I am looking for a chemical compound which was first described in a plant in 1914. Because the substance is not commercially available anywhere, I would like to get some essential oil from this plant, but I don't know where from. The name of the plant is ELSHOLTZIA CRISTATA (Willd.) or E. CILIATA (Thunb.). It comes from Japan and is known there as NAGINATA-KOUJU. A decoction of the leaves is used in Japan to relieve the effects of excess alcohol and might still be used. Thank you for any help. Please write to my e-mail address. -Mario. (e-mail: Mario.Salamon@zoo.utas.edu.au) From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!spool.mu.edu!olivea!decwrl!decwrl!waikato!waikato.ac.nz!fri.cri.nz!cokera From: cokera@fri.cri.nz Newsgroups: bionet.plants Subject: Ph.D scholarship, NEW ZEALAND Message-ID: <1993Dec6.113359.768@fri.cri.nz> Date: 5 Dec 93 22:33:59 GMT Organization: New Zealand Forest Research Institute Lines: 82 PhD SCHOLARSHIP OFFERED IN NEW ZEALAND 1994 CYTOKININS -REGULATORS OF JUVENILITY AND MATURATION IN PINUS RADIATA ? Maturation as a developmental process is poorly understood despite the considerable significance age manipulation plays in the genetic improvement of radiata pine. Superior radiata pine cannot be identified until seven to eight years of age when maturation characteristics are expressed. Cuttings taken at maturity exhibit undesirable properties for propagation including a reduction in diameter growth and rooting ability. From evidence to date it appears that maturation occurs within the bud as a consequence of self-programming and a combination of inter- and intracellular signalling. Over a period of time, the bud acquires a certain individuality with "memory " of age persisting even after excision from the plant for vegetative propagation. This forms the basis of the mature micropropagation procedure developed at New Zealand Forest Research Institute and offers a novel system for manipulating and understanding age-related events. In this system , exogenous cytokinins cause a "rejuvenation " of mature P.radiata in vitro, at least initially. Cytokinins are known to be important regulators of growth and development . The morphological and physiological phenotype characteristic of a cytokinin effect, represents a suite of events over a period of time overlaid on a framework of biochemical and molecular events. The aim of this project will be to determine whether cytokinins are involved in the determination of the juvenile/mature phenotype and studies will be carried out to: - analyse endogenous cytokinins in mature and juvenile material by HPLC and radioimmunoassays - compare cytokinin metabolism in juvenile and mature tissues - manipulate the endogenous cytokinin complement through transformation studies to investigate in vivo expression of a cytokinin gene The project will be jointly supervised at New Zealand Forest Research Institute by Ms Astrid Coker and Ms Kathryn Horgan and at the Department of Plant Biology and Biotechnology , Massey University Palmerston North,NZ by Dr Paula Jameson. Enquiries and applications can be made to Dr Paula Jameson, Dept Plant Biology and Biotechnology, Massey University, Private Bag 11222, Palmerston North NZ. FAX: +64 6 350 5623 TELEPHONE: +64 6 356 9099, extension 7518. OR CokerA@tawa.fri.cri.nz Applications should include a copy of your academic record, a description of research experience and interests and names of two referees. Closing date : 18th January 1994 From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!munnari.oz.au!newsroom.utas.edu.au!zoology49h38.zoo.utas.edu.au!user From: Mario.Salamon@zoo.utas.edu.au (Mario Salamon) Newsgroups: bionet.plants Subject: HELP: Looking for Japanese plant oil Message-ID: Date: 6 Dec 93 00:46:08 GMT Followup-To: bionet.plants Organization: University of Tasmania Lines: 20 NNTP-Posting-Host: zoology49h38.zoo.utas.edu.au Hello out there, I am looking for a chemical compound which was first described in a plant in 1914. Because the substance is not commercially available anywhere, I would like to get some essential oil from this plant, but I don't know where from. The name of the plant is ELSHOLTZIA CRISTATA (Willd.) or E. CILIATA (Thunb.). It comes from Japan and is known there as NAGINATA-KOUJU. A decoction of the leaves is used in Japan to relieve the effects of excess alcohol and might still be used. Thank you for any help. Please write to my e-mail address. -Mario. (e-mail: Mario.Salamon@zoo.utas.edu.au) From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!lhc!darwin.sura.net!europa.eng.gtefsd.com!uunet!munnari.oz.au!newsroom.utas.edu.au!zoology49h38.zoo.utas.edu.au!user From: Mario.Salamon@zoo.utas.edu.au (Mario Salamon) Newsgroups: bionet.plants Subject: HELP: Looking for Japanese plant oil Message-ID: Date: 6 Dec 93 00:46:08 GMT Followup-To: bionet.plants Organization: University of Tasmania Lines: 20 NNTP-Posting-Host: zoology49h38.zoo.utas.edu.au Hello out there, I am looking for a chemical compound which was first described in a plant in 1914. Because the substance is not commercially available anywhere, I would like to get some essential oil from this plant, but I don't know where from. The name of the plant is ELSHOLTZIA CRISTATA (Willd.) or E. CILIATA (Thunb.). It comes from Japan and is known there as NAGINATA-KOUJU. A decoction of the leaves is used in Japan to relieve the effects of excess alcohol and might still be used. Thank you for any help. Please write to my e-mail address. -Mario. (e-mail: Mario.Salamon@zoo.utas.edu.au) From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!lhc!darwin.sura.net!spool.mu.edu!olivea!decwrl!decwrl!waikato!waikato.ac.nz!fri.cri.nz!cokera From: cokera@fri.cri.nz Newsgroups: bionet.plants Subject: Ph.D scholarship, NEW ZEALAND Message-ID: <1993Dec6.113359.768@fri.cri.nz> Date: 5 Dec 93 22:33:59 GMT Organization: New Zealand Forest Research Institute Lines: 82 PhD SCHOLARSHIP OFFERED IN NEW ZEALAND 1994 CYTOKININS -REGULATORS OF JUVENILITY AND MATURATION IN PINUS RADIATA ? Maturation as a developmental process is poorly understood despite the considerable significance age manipulation plays in the genetic improvement of radiata pine. Superior radiata pine cannot be identified until seven to eight years of age when maturation characteristics are expressed. Cuttings taken at maturity exhibit undesirable properties for propagation including a reduction in diameter growth and rooting ability. From evidence to date it appears that maturation occurs within the bud as a consequence of self-programming and a combination of inter- and intracellular signalling. Over a period of time, the bud acquires a certain individuality with "memory " of age persisting even after excision from the plant for vegetative propagation. This forms the basis of the mature micropropagation procedure developed at New Zealand Forest Research Institute and offers a novel system for manipulating and understanding age-related events. In this system , exogenous cytokinins cause a "rejuvenation " of mature P.radiata in vitro, at least initially. Cytokinins are known to be important regulators of growth and development . The morphological and physiological phenotype characteristic of a cytokinin effect, represents a suite of events over a period of time overlaid on a framework of biochemical and molecular events. The aim of this project will be to determine whether cytokinins are involved in the determination of the juvenile/mature phenotype and studies will be carried out to: - analyse endogenous cytokinins in mature and juvenile material by HPLC and radioimmunoassays - compare cytokinin metabolism in juvenile and mature tissues - manipulate the endogenous cytokinin complement through transformation studies to investigate in vivo expression of a cytokinin gene The project will be jointly supervised at New Zealand Forest Research Institute by Ms Astrid Coker and Ms Kathryn Horgan and at the Department of Plant Biology and Biotechnology , Massey University Palmerston North,NZ by Dr Paula Jameson. Enquiries and applications can be made to Dr Paula Jameson, Dept Plant Biology and Biotechnology, Massey University, Private Bag 11222, Palmerston North NZ. FAX: +64 6 350 5623 TELEPHONE: +64 6 356 9099, extension 7518. OR CokerA@tawa.fri.cri.nz Applications should include a copy of your academic record, a description of research experience and interests and names of two referees. Closing date : 18th January 1994 From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!howland.reston.ans.net!newsserver.jvnc.net!netnews.upenn.edu!dsinc!vu-vlsi.ee.vill.edu!shrsys.hslc.org!frdschlib From: frdschlib@shrsys.hslc.org Newsgroups: bionet.plants Subject: serpentine root Message-ID: <6DEC93.17482326@shrsys.hslc.org> Date: 6 Dec 93 22:48:23 GMT Sender: news@vu-vlsi.ee.vill.edu Organization: MIT PLASMA FUSION CENTER Lines: 6 I am looking for info. on the following plants and their medicinal uses: serpentine root, diosgenin, quinine, and vincristine Thanks Sanna frdschlib@hslc.org From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!news.cs.umb.edu!hsdndev!yale!yale.edu!spool.mu.edu!howland.reston.ans.net!europa.eng.gtefsd.com!uunet!munnari.oz.au!newsroom.utas.edu.au!zoology49h38.zoo.utas.edu.au!user From: Mario.Salamon@zoo.utas.edu.au (Mario Salamon) Newsgroups: bionet.plants Subject: HELP: Looking for Japanese plant oil Message-ID: Date: 6 Dec 93 00:46:08 GMT Followup-To: bionet.plants Organization: University of Tasmania Lines: 20 NNTP-Posting-Host: zoology49h38.zoo.utas.edu.au Hello out there, I am looking for a chemical compound which was first described in a plant in 1914. Because the substance is not commercially available anywhere, I would like to get some essential oil from this plant, but I don't know where from. The name of the plant is ELSHOLTZIA CRISTATA (Willd.) or E. CILIATA (Thunb.). It comes from Japan and is known there as NAGINATA-KOUJU. A decoction of the leaves is used in Japan to relieve the effects of excess alcohol and might still be used. Thank you for any help. Please write to my e-mail address. -Mario. (e-mail: Mario.Salamon@zoo.utas.edu.au) From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!news.cs.umb.edu!hsdndev!yale!yale.edu!newsserver.jvnc.net!howland.reston.ans.net!spool.mu.edu!olivea!decwrl!decwrl!waikato!waikato.ac.nz!fri.cri.nz!cokera From: cokera@fri.cri.nz Newsgroups: bionet.plants Subject: Ph.D scholarship, NEW ZEALAND Message-ID: <1993Dec6.113359.768@fri.cri.nz> Date: 5 Dec 93 22:33:59 GMT Organization: New Zealand Forest Research Institute Lines: 82 PhD SCHOLARSHIP OFFERED IN NEW ZEALAND 1994 CYTOKININS -REGULATORS OF JUVENILITY AND MATURATION IN PINUS RADIATA ? Maturation as a developmental process is poorly understood despite the considerable significance age manipulation plays in the genetic improvement of radiata pine. Superior radiata pine cannot be identified until seven to eight years of age when maturation characteristics are expressed. Cuttings taken at maturity exhibit undesirable properties for propagation including a reduction in diameter growth and rooting ability. From evidence to date it appears that maturation occurs within the bud as a consequence of self-programming and a combination of inter- and intracellular signalling. Over a period of time, the bud acquires a certain individuality with "memory " of age persisting even after excision from the plant for vegetative propagation. This forms the basis of the mature micropropagation procedure developed at New Zealand Forest Research Institute and offers a novel system for manipulating and understanding age-related events. In this system , exogenous cytokinins cause a "rejuvenation " of mature P.radiata in vitro, at least initially. Cytokinins are known to be important regulators of growth and development . The morphological and physiological phenotype characteristic of a cytokinin effect, represents a suite of events over a period of time overlaid on a framework of biochemical and molecular events. The aim of this project will be to determine whether cytokinins are involved in the determination of the juvenile/mature phenotype and studies will be carried out to: - analyse endogenous cytokinins in mature and juvenile material by HPLC and radioimmunoassays - compare cytokinin metabolism in juvenile and mature tissues - manipulate the endogenous cytokinin complement through transformation studies to investigate in vivo expression of a cytokinin gene The project will be jointly supervised at New Zealand Forest Research Institute by Ms Astrid Coker and Ms Kathryn Horgan and at the Department of Plant Biology and Biotechnology , Massey University Palmerston North,NZ by Dr Paula Jameson. Enquiries and applications can be made to Dr Paula Jameson, Dept Plant Biology and Biotechnology, Massey University, Private Bag 11222, Palmerston North NZ. FAX: +64 6 350 5623 TELEPHONE: +64 6 356 9099, extension 7518. OR CokerA@tawa.fri.cri.nz Applications should include a copy of your academic record, a description of research experience and interests and names of two referees. Closing date : 18th January 1994 From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!lhc!darwin.sura.net!howland.reston.ans.net!cs.utexas.edu!uunet!caen!malgudi.oar.net!witch!tytownms!mbridges From: mbridges@tytownms.win.net (Michael H. Bridges) Newsgroups: bionet.plants Subject: Re: Plant cuttings. Message-ID: <164@tytownms.win.net> Date: 5 Dec 93 16:25:25 GMT References: <93339.010207TLC112@psuvm.psu.edu> Reply-To: mbridges@tytownms.win.net (Michael H. Bridges) Lines: 25 In article <93339.010207TLC112@psuvm.psu.edu>, The One and Only (TLC112@psuvm.psu.edu) writes: >Ok, simple stuff. I realize that I'm way out of league here, but.... >Where, in relation to the node should one cut a house plant in order to root >it? In my experience, it doesn't matter, though cutting slightly below a node, and making sure the node is at or below the soil surface when the cutting is stuck, is the recommended method. >Also, has anyone had any experience with "crystal soil" jello looking potting >mix? Is it worth a try? Not as a pure potting mix, but as an additive to a commercial mix, possibly. The stuff is really too expensive to justify its use for me (commercially), but you may find it useful on valuable plants that you sometimes neglect to water properly. Michael Bridges mbridges@tytownms.win.net Southern Perennials & Herbs Tylertown, Mississippi (Wholesale Nursery) (601)684-1769 From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!news.cs.umb.edu!hsdndev!yale!yale.edu!newsserver.jvnc.net!darwin.sura.net!howland.reston.ans.net!cs.utexas.edu!uunet!caen!malgudi.oar.net!witch!tytownms!mbridges From: mbridges@tytownms.win.net (Michael H. Bridges) Newsgroups: bionet.plants Subject: Re: Plant cuttings. Message-ID: <164@tytownms.win.net> Date: 5 Dec 93 16:25:25 GMT References: <93339.010207TLC112@psuvm.psu.edu> Reply-To: mbridges@tytownms.win.net (Michael H. Bridges) Lines: 25 In article <93339.010207TLC112@psuvm.psu.edu>, The One and Only (TLC112@psuvm.psu.edu) writes: >Ok, simple stuff. I realize that I'm way out of league here, but.... >Where, in relation to the node should one cut a house plant in order to root >it? In my experience, it doesn't matter, though cutting slightly below a node, and making sure the node is at or below the soil surface when the cutting is stuck, is the recommended method. >Also, has anyone had any experience with "crystal soil" jello looking potting >mix? Is it worth a try? Not as a pure potting mix, but as an additive to a commercial mix, possibly. The stuff is really too expensive to justify its use for me (commercially), but you may find it useful on valuable plants that you sometimes neglect to water properly. Michael Bridges mbridges@tytownms.win.net Southern Perennials & Herbs Tylertown, Mississippi (Wholesale Nursery) (601)684-1769 From owner-plants@net.bio.net Sun Dec 05 22:00:00 1993 Path: biosci!news.cs.umb.edu!hsdndev!yale!yale.edu!spool.mu.edu!sgiblab!munnari.oz.au!bunyip.cc.uq.oz.au!dpi-gw!qdpii!ianst From: ianst@qdpii.ind.dpi.qld.gov.au (Ian Staples) Newsgroups: bionet.plants Subject: Why no chromosome numbers? [Was: Onion Chromosomes] Message-ID: Date: 5 Dec 93 07:48:42 GMT References: <1993Dec2.142019.6956@msus1.msus.edu> <1993Dec3.093633.1919@rhrk.uni-kl.de> Organization: Queensland Dept of Primary Industries Lines: 38 >> I am a graduate assistant in biology, and we need to know the number of chromosomes in the onion. We cannot find it anywhere, and know one here knows tha answer. It is for a general biology lab. From last quarters data I would have to say the number is 10. Can anyone confirm this? [There seems to be a severe shortage of line endings at St. Cloud ;-) ] >> Thank you in advance. >> KR Ziarkowski >> St. Cloud State University >> St. Cloud, MN Does this mean the "chromosome bible" of my youth is out of print? :-( Namely: Darlington, C.D. and Wylie, A.P. (1955) _Chromosome Atlas of Flowering Plants._ (London: George Allen & Unwin Ltd). Pp. xix+519 Cheers, Ian S. P.S. Not having D&W at my side, I checked out several floras, dictionaries of flowering plants, manuals of cultivated plants, and a few plant breeding and cytology & cytogenetics texts - I hadn't realised how badly we are served when it comes to such a fundamental thing as chromosome number! In 9 such texts scanned, the best I could find was _Allium_ n=7,8,9. Nothing for onion _per se_ (_A. cepa_). Finally, in the 10th book (Winchester, A.M. 1958(!) - _Genetics_. 2nd ed. (Boston: Houghton Mifflin Co.) Pp. xiii+413) I found an off-hand reference in the caption to fig. 15-11 (concerning the effect of colchicine on chromosome doubling in root tips): "There are 28 rather than the normal 14 chromosomes ..." So I guess n=7 for _A. cepa_. I find it absurd that chromosome numbers are not quoted more commonly in floras, manuals, and similar compendia. Nor even in plant breeding, cytology, and similar texts where individual species are often used as examples and illustrations for various phenomena and techniques. -- Ian Staples E-mail : ianst@qdpii.ind.dpi.qld.gov.au c/- P.O. Box 1054 MAREEBA Phone : +61 (0)70 921 555 Home 924 847 Queensland Australia 4880 Fax : +61 (0)70 923 593 " " " From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!haven.umd.edu!darwin.sura.net!gatekeeper.es.dupont.com!chemsci1.es.dupont.com!chem129.es.dupont.com!meyerdt From: meyerdt@chemsci1.es.dupont.com (Dayton Meyer) Newsgroups: bionet.plants Subject: Zoysia Grass Help Message-ID: <2e21kd$3qf@chemsci1.es.dupont.com> Date: 7 Dec 93 13:49:01 GMT Organization: DuPont Merck Pharmaceutical Company Lines: 8 NNTP-Posting-Host: chem129 X-UserAgent: Nuntius v1.1.1d17 X-XXMessage-ID: X-XXDate: Tue, 7 Dec 93 00:51:15 GMT I am in sincere need of any information, opinion or advice on Zoysia grass. Any help, small or large, is infinitely appreciated. Thanks. E-mail only, please. meyerdt@chemsci1.es.dupont.com From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!europa.eng.gtefsd.com!howland.reston.ans.net!spool.mu.edu!wupost!waikato!comp.vuw.ac.nz!news.massey.ac.nz!cc-server4.massey.ac.nz!CChristi From: CChristi@massey.ac.nz (C.B. Christie) Newsgroups: bionet.plants Subject: Numerical analysis: how do yo do it? Message-ID: Date: 7 Dec 93 04:40:09 GMT Followup-To: sci.bio Organization: Massey University, Palmerston North, New Zealand Lines: 16 X-Reader: NETNEWS/PC Version 2f I am looking for a bit of software or even just the methodology for conducting a numerical analysis to identify the similarity of plants sharing bands on a gel after RAPD analysis. Any suggestions welcomed. Thanks very much Bruce Christie Department of Plant Science, Massey University Palmerston North, New Zealand email: C.B.Christie@massey.ac.nz From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!daresbury!bioftp.unibas.ch!rc1!ub4b!EU.net!howland.reston.ans.net!newsserver.jvnc.net!netnews.upenn.edu!news.cc.swarthmore.edu!mac08.whartoncd.swarthmore.edu!user From: kwestin1 Newsgroups: bionet.plants Subject: Sassafra Leaf Morph distribution Message-ID: Date: 7 Dec 93 19:43:30 GMT Followup-To: bionet.plants Organization: Swarthmore College, Swarthmore, PA, USA Lines: 8 NNTP-Posting-Host: mac08.whartoncd.swarthmore.edu Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit Two undergrads at Swarthmore College are looking at the distribution of three leaf morph shapes over a range of trees, species Sassafras albidum. We've found a strong correlation between percent unlobed leaves and age - the older the entire tree (not just the branch), the more unlobed leaves it has. A literature search has turned up very little and we're brainstormed out. Any ideas, however wild, entertained! Can _you_ think of a reason for the correlation? Responses, questions sent to kwestin1@cc.swarthmore.edu Many thanks! From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!MAROON.TC.UMN.EDU!darve002 From: darve002@MAROON.TC.UMN.EDU (Bettina Darveaux-1) Newsgroups: bionet.plants Subject: (none) Message-ID: Date: 7 Dec 93 22:51:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 6 Hi,I am wondering if anyone knows where I can obtain some C14 labeled picloram (4-amino-3,5,6-trichloropicolinic acid). Picloram is a herbicide manifactured by Dow Chemical Co. under the trademark name of Tordon. Chemical suppliers such as Sigma and Aldrich do not carry the radiolabeled picloram. I would appreciate your help. Thanks, Bettina. From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!JUDY.ENG.UCI.EDU!liang From: liang@JUDY.ENG.UCI.EDU (liang) Newsgroups: bionet.plants Subject: CHINESES_BIOTECH_NET_FOUNDED Message-ID: <9312060501.AA19439@judy.eng.uci.edu> Date: 6 Dec 93 05:01:14 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 12 CBNet (Chinese Biotechnology Network) is a non-profit organization composed of professionals in biological, chemical, medical sciences, engineering and related fields. The CBNet sponsors the Chinese Biotechnology Internet Forum (CBIF) newsletter. To subscribe CBIF, please send an email to Listserv@UCSD.Edu with the message body: Add CB-Net. From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!lhc!darwin.sura.net!paladin.american.edu!howland.reston.ans.net!pipex!uknet!gdt!aber!spp From: spp@aber.ac.uk (Simon Peter Penson) Newsgroups: bionet.plants Subject: Antibody to plant sucrase/fructosyl transferase Summary: Antibody. fructan Message-ID: <1993Dec6.162212.26197@aber.ac.uk> Date: 6 Dec 93 16:22:12 GMT Sender: Simon Penson (spp@aber.ac.uk) Distribution: bionet.plants, bionet.immunology, bionet.molbio.proteins Organization: University of Wales, Aberystwyth Lines: 5 I'm after an antibody to wheat fructosyl transferase/invertase proteins. Does anybody know of such a thing (or a bacterial equivalent)? Simon Penson From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!JUDY.ENG.UCI.EDU!liang From: liang@JUDY.ENG.UCI.EDU (liang) Newsgroups: bionet.plants Subject: CHINESES_BIOTECH_NET_FOUNDED Message-ID: <9312061946.AA20542@judy.eng.uci.edu> Date: 6 Dec 93 19:46:10 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 12 CBNet (Chinese Biotechnology Network) is a non-profit organization composed of professionals in biological, chemical, medical sciences, engineering and related fields. The CBNet sponsors the Chinese Biotechnology Internet Forum (CBIF) newsletter. To subscribe CBIF, please send an email to Listserv@UCSD.Edu with the message body: Add CB-Net. From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!olivea!spool.mu.edu!umn.edu!msus1.msus.edu!TIGGER.STCLOUD.MSUS.EDU!J00030 From: j00030@TIGGER.STCLOUD.MSUS.EDU Newsgroups: bionet.plants Subject: small everlasting antennaria Message-ID: <1993Dec6.131844.7071@msus1.msus.edu> Date: 6 Dec 93 19:18:43 GMT Reply-To: j00030@TIGGER.STCLOUD.MSUS.EDU Organization: ST. CLOUD STATE UNIVERSITY, ST. CLOUD, MN Lines: 7 Nntp-Posting-Host: tigger.stcloud.msus.edu I tried email to you and get invalid user specification. Here at the SCSU greenhouse we use Oasis growing medium-Wedge. They work very well and we have success with most every cutting we have tried in them. I noticed that they must not dry out, so use a cutting bench. Secondly, I would like to introduce myself to your greenhouse supervisor. I am working on my Masters in Biology and will graduate in May. I have applied for admission to USD to earn my PhD. At SCSU I am the graduate student greenhouse supervisor. I assist the Professor in charge in all aspects of our greenhouse. I have management and marketing skills (we have sales here to raise funds),and computer skills (I have computerized our inventory). If I am accepted atUSD I will be interested in working in the greenhouse. Thank you; Kim Robert ("KR") Ziarkowski From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!pipex!demon!zenecabp.demon.co.uk!sbungard From: sbungard@zenecabp.demon.co.uk (Stephen J Bungard) Newsgroups: bionet.plants Subject: Atriplex Message-ID: <755216473snz@zenecabp.demon.co.uk> Date: 6 Dec 93 22:21:13 GMT Sender: usenet@demon.co.uk Reply-To: sbungard@zenecabp.demon.co.uk Organization: ZENECA Bio Products Lines: 12 X-Newsreader: Simple NEWS 2.0 (ka9q DIS 1.24) Sorry, I have left this so long the original request has been lost so I have to post this here. British Atriplex are well illustrated in an article by P M Taschereau in Watsonia 15 183-209 (1985) - line drawings not photo's. Taschereau is reachable c/o Inst for Resource & Environmental Studies, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3E2. Sorry no e-mail address known -- Stephen J Bungard aka sbungard@zenecabp.demon.co.uk (*) If there are opinions in the above, | they are mine, all mine. __\|/__ From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!GPU.SRV.UALBERTA.CA!lmaret From: lmaret@GPU.SRV.UALBERTA.CA (Lol Maret) Newsgroups: bionet.plants Subject: Re: Plant cuttings. Message-ID: Date: 5 Dec 93 20:38:11 GMT References: <9312050644.AA23699@net.bio.net> Sender: daemon@net.bio.net Distribution: bionet Lines: 26 On 5 Dec 1993, The One and Only wrote: > Date: 5 Dec 93 06:02:07 GMT > From: The One and Only > To: plant-biology@net.bio.net > Subject: Plant cuttings. > > Ok, simple stuff. I realize that I'm way out of league here, but.... > > Where, in relation to the node should one cut a house plant in order to root > it? > > Also, has anyone had any experience with "crystal soil" jello looking potting > mix? Is it worth a try? > > Thanks.... > Terry > > Simple questions are ok too. You don't say what kind of houseplant you want to root. Usually, the best spot is just below a node for plants such as ivy or pothos etc. Lots of good references for specific plants are available at your local plant store or library. Experiment with a few techniques and see what works best! Good luck :-) From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!europa.eng.gtefsd.com!howland.reston.ans.net!spool.mu.edu!umn.edu!msus1.msus.edu!TIGGER.STCLOUD.MSUS.EDU!DEGROOTE From: degroote@TIGGER.STCLOUD.MSUS.EDU Newsgroups: bionet.plants Subject: plant tissue s-levels? Message-ID: <1993Dec6.192534.7074@msus1.msus.edu> Date: 7 Dec 93 01:25:33 GMT Reply-To: degroote@TIGGER.STCLOUD.MSUS.EDU Organization: ST. CLOUD STATE UNIVERSITY, ST. CLOUD, MN Lines: 5 Nntp-Posting-Host: tigger.stcloud.msus.edu A student in my Plant Physiology class is interested in looking at tissue levels of sulfur in various tissue from plants grown on complete and low sulfur media. Any assistance would be appreciated. David DeGroote St. Cloud State University From owner-plants@net.bio.net Mon Dec 06 22:00:00 1993 Path: biosci!am.abru.cg.com!DELANEYT From: DELANEYT@am.abru.cg.com (DELANEYT 919-541-8577) Newsgroups: bionet.plants Subject: (none) Message-ID: <8342030906121993.A22787.YOMAMA.117C32432700*@abru.cg.com> Date: 6 Dec 93 19:03:42 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 22 "Terry" from TLC112@psuvm.psu.edu writes: >...Ok, simple stuff. I realize that I'm way out of league here, but.... > >Where, in relation to the node should one cut a house plant in order to root >it?.... ============================================================================== In case you aren't inundated with replies, I'll offer: cut the plant just beneath the node (5-10 mm), and strip off the leaves next to the cut for a distance of 4-8 cm, leaving several leaves at the tip of the shoot (maybe 8-15 cm). For best results, dust the base of the cutting in an artificial auxin, such as Rootone, and plant in well draining soil. Keep somewhat humid, and keep the soil moist but not wet and you should have good results. Conditions vary between plants. For lots more information, you might look at Plant Prepagation, Principles and Practices, by Hartmann and Kester, 1975 (3rd ed). Good luck, Terry Delaney Ciba Geigy Agric Biotech From owner-plants@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!imb.lan.nrc.ca!RossN From: RossN@imb.lan.nrc.ca Newsgroups: bionet.plants Subject: high fructose corn syrup Message-ID: <2D05D9FA@coursmtp.nrc.ca> Date: 8 Dec 93 13:40:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 23 Here is my limited understanding of how high-fructose corn syrup is made. Corn starch is hydrolyzed to glucose which is then enzymatically converted to fructose by glucose isomerase (aka xylose isomerase). The enzymatic step is used since fructose is several times sweeter than glucose on a per weight basis. I don't know how the starch is hydrolyzed (acid hydrolysis versus amylase digestion). I hope this helps. Neil Ross ROSSN@imb.lan.nrc.ca ------------------------------------------------------------------------------ FORWARDED FROM: Ross, Neil W. Return-Path: Message-Id: <9312040914.AA08742@net.bio.net> To: plant-biology@net.bio.net From: degroote@TIGGER.STCLOUD.MSUS.EDU Subject: high fructose corn syrup Date: 4 Dec 93 04:37:34 GMT Reply-To: degroote@TIGGER.STCLOUD.MSUS.EDU Nntp-Posting-Host: tigger.stcloud.msus.edu The ethnobotanist asked my today how they get the high-fructose in corn syrup these days. He indicated that the old karo was not all that sweet but now the corn syrup is used as a sweetener all over. Any info would be appreciated Davide From owner-plants@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!howland.reston.ans.net!usenet.ins.cwru.edu!nigel.msen.com!math.fu-berlin.de!news.dfn.de!solaris.rz.tu-clausthal.de!newsserver.rrzn.uni-hannover.de!oeko2.ifgb.uni-hannover.de!Niels.Sommer From: Niels.Sommer@cdc2.gartenbauoekonomie.gartenbau.uni-hannover.d400.de (Niels Sommer) Newsgroups: bionet.plants Subject: horticultural internet resources !? Message-ID: Date: 7 Dec 93 15:09:35 GMT Sender: news@newsserver.rrzn.uni-hannover.de (News Service) Organization: Regionales Rechenzentrum fr Hannover Lines: 58 horticultural Internet resources !? We send a list of interesting horticultural Internet resources. The accesses are free. But we think this list is not complete. We try to compile a complete list with all Internet resources who are interesting for all gardeners, these resources haven't been free, also addresses with costs are needed. So, please send us your adresses, informations and comments (via mail). We try to send the new list in this newsgroup, is the list too long we give you informations where you can get it, thanks Angela and Niels And here the uncomplete list of adresses: TELNET: PENpages psupen.psu.edu, login: pnopta CENET cce.cornell.edu, login: guest CUFAN eureka.clemson.edu, login: PUBLIC CSU FRESNO ATI-NET caticsuf.csufresno.edu, login: super Agriculture info idea.ag.uiuc.edu, login: interag GOPHER: search in Veronica Agrargopher in sweden USDA Current Research Information System rice.info.rice.edu WAIS: agricultural-market-news.src usda-rrdb.src WWW-Server: Australian National Botanic Garden INRA **************************************************************************** Niels Sommer Institut fuer Gartenbauoekonomie Herrenhaeuser Strasse 2 30419 Hannover Germany Telefon: 0511-762-3630 Telefax: 0511-762-2667 E-MAIL: Niels.Sommer@cdc2.gartenbauoekonomie.gartenbau.uni-hannover.d400.de **************************************************************************** From owner-plants@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!chempath.uct.ac.za!NIGEL From: NIGEL@chempath.uct.ac.za ("Nigel Barker") Newsgroups: bionet.plants Subject: African Biodiversity Network Message-ID: Date: 8 Dec 93 11:16:31 GMT Sender: daemon@net.bio.net Reply-To: nigel@chempath.uct.ac.za Lines: 120 Hi netters... I am posting this on behalf of the ABN listserver supervisor. Please respond as directed if you are interested. Nigel Barker *********** ANNOUNCING A NEW E- MAIL LISTSERVER ************* THE AFRICAN BIODIVERSITY NETWORK MEMBERSHIP of the network is not restricted in any way. The network has been initiated to help African conservation biologists exchange information and keep in contact. Examples of its use are requests for and offers of information, ideas, data, advertising employment, bursary, fellowship and volunteer opportunities, notices of meetings, availability of written materials (e.g. reports, books, proceedings, reprints), etc. All e-mail messages sent out on the network by members will automatically be sent to all other members. The African Biodiversity Network has been initiated by Mandy Lombard, Percy FitzPatrick Institute of African Ornithology, University of Cape Town, Rondebosch 7700, South Africa (alombard@zoo.uct.ac.za). The initial announcement of the network was sent in September 1993 to conservation biologists throughout South Africa, who are personally known to Mandy. The network will be extended to all other interested persons, and biologists throughout Africa, in time, with YOUR help. HOWEVER, receipt of this message does NOT mean that you have automatically been made a member of the network. Your privacy is being fully respected and your e-mail and other addresses will be added to the network's address list (and therefore be available to all network members) ONLY if you join the network as detailed below. To join the network send to BIODIVE@ZOO.UCT.AC.ZA the following PHRASE in the SUBJECT FIELD of an e-mail letter SUBSCRIBE BIODIVE and in the body of the e-mail letter your full name and postal, telephone and fax addresses as you would like them listed in the network's address list (see below). Leaving these out will mean that you will appear in the address list under your e-mail address only. If you so desire you may also list your research interests as well for inclusion with the address list. If you know of others who would like to be join the network ask them to send their own subscription requests to BIODIVE@ZOO.UCT.AC.ZA To send your own messages on the network enter the address BIODIVE@ZOO.UCT.AC.ZA place a subject heading of your choice in the subject field and enter your message in the main body below. Also available is a archival file which contains all the e-mail messages sent on the network to date. This is available by sending in the SUBJECT FIELD of an e-mail letter to BIODIVE@ZOO.UCT.AC.ZA ARCHIVE this will automatically send you a file containing all e-mail messages to date. Also available is an address list which will contain all the current members of the network. It is automatically accessible by sending in the SUBJECT FIELD of an e-mail letter to BIODIVE@ZOO.UCT.AC.ZA ADDRESSES Features of the African Conservation Biologist's Network: CONTINENTAL MEMBERSHIP AUTOMATIC DISTRIBUTION OF E-MAIL & FILES TO ALL MEMBERS ARCHIVING OF ALL MESSAGES ACCESS TO ALL MEMBERS' FULL ADDRESSES Possible future developements: STORAGE AND ACCESS TO DATA FILES ABSTRACTED INFORMATION OF EACH MEMBER AND THEIR RESEARCH INTERESTS, DATABASES HELD etc. ------------------------------------------------ Amanda T. Lombard FitzPatrick Institute of African Ornithology University of Cape Town, Private Bag Rondebosch Cape Town 7700, South Africa Tel: 27-21-6504026 Fax: 27-21-6503295 alombard@zoo.uct.ac.za ------------------------------------------------- Nigel P. Barker Dept. of Botany ____ __ University of Cape Town / \--/ \_ P. Bag | / Rondebosch \___ / 7700 \ | Nkosi Sikelel i'Afrika South Africa | | Fax: +21 650 3726 \_/-\/ Lord, bless Africa \__/ Nigel@chempath.uct.ac.za Dragon@botany.uct.ac.za From owner-plants@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!CS.Arizona.EDU!organpipe.uug.arizona.edu!uunet!cs.utexas.edu!howland.reston.ans.net!agate!dog.ee.lbl.gov!overload.lbl.gov!deh From: deh@s27w007.pswfs.gov (Dave Harry) Newsgroups: bionet.plants Subject: Re: Sassafra Leaf Morph distribution Message-ID: <2e55fi$sm@overload.lbl.gov> Date: 8 Dec 93 18:13:06 GMT References: Organization: Dendrome, A Genome Database for Forest Trees Lines: 32 NNTP-Posting-Host: s27w007.pswfs.gov In article kwestin1 writes: >Two undergrads at Swarthmore College are looking at the distribution of >three leaf morph shapes over a range of trees, species Sassafras albidum. >We've found a strong correlation between percent unlobed leaves and age - >the older the entire tree (not just the branch), the more unlobed leaves it >has. A literature search has turned up very little and we're brainstormed >out. Any ideas, however wild, entertained! Can _you_ think of a reason >for the correlation? Responses, questions sent to >kwestin1@cc.swarthmore.edu Many thanks! I can't speak to this specific problem, but there are many examples (some published, even more observational or anecdotal) of developmental trends in woody plants related to maturation. One simple explanation might be that leaves w/o lobes tend to be more frequent as trees mature (which you've already observed). Age and maturation state are not perfectly correlated, however. Other examples of maturation-related phenomena include the relative abundance of male vs female reproductive structures (on monoecious species such as conifers), rooting ability, etc. You might check the literature on woody plants using search terms such as maturation, juvenility, rejuvenation. Two authors who come to mind are Michael Greenwood and William J. Libby. Good luck! David Harry Institute of Forest Genetics deh@s27w007.pswfs.gov USDA Forest Service, Pacific SW Station Phone: 510/559-6439 PO Box 245 FAX: 510/559-6499 Berkeley, CA 94701 My failure to mention other authors is simply due to ignorance and negligence, not to malicious intent. :-) From owner-plants@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!imb.lan.nrc.ca!RossN From: RossN@imb.lan.nrc.ca Newsgroups: bionet.plants Subject: Re: Sassafra Leaf Morph distribution Message-ID: <2D0645D3@coursmtp.nrc.ca> Date: 8 Dec 93 21:10:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 52 This has probably been stated before, but here goes. In my (non-scientific) observations of maple and oak trees of eastern Canada, I have noted that the more light a leaf sees the deeper the lobes on the leaf. Shaded leaves of a mature maple are more "rounded" than leaves from the top of the same tree. Could a similar phenomenom explain the lobes in the Sassafras tree? Could this be tested by shading selected branches on a tree at the beginning of a growing season and compare with unshaded branches? Just my two cents worth. Neil (If my statements appear naive, it's because they are) ------------------------------------------------------------------------------ FORWARDED FROM: Ross, Neil W. Return-Path: Message-Id: <9312081843.AA26710@net.bio.net> To: plant-biology@net.bio.net From: deh@s27w007.pswfs.gov (Dave Harry) Subject: Re: Sassafra Leaf Morph distribution Date: 8 Dec 93 18:13:06 GMT Nntp-Posting-Host: s27w007.pswfs.gov In article kwestin1 writes: >Two undergrads at Swarthmore College are looking at the distribution of >three leaf morph shapes over a range of trees, species Sassafras albidum. >We've found a strong correlation between percent unlobed leaves and age - >the older the entire tree (not just the branch), the more unlobed leaves it >has. A literature search has turned up very little and we're brainstormed >out. Any ideas, however wild, entertained! Can _you_ think of a reason >for the correlation? Responses, questions sent to >kwestin1@cc.swarthmore.edu Many thanks! I can't speak to this specific problem, but there are many examples (some published, even more observational or anecdotal) of developmental trends in woody plants related to maturation. One simple explanation might be that leaves w/o lobes tend to be more frequent as trees mature (which you've already observed). Age and maturation state are not perfectly correlated, however. Other examples of maturation-related phenomena include the relative abundance of male vs female reproductive structures (on monoecious species such as conifers), rooting ability, etc. You might check the literature on woody plants using search terms such as maturation, juvenility, rejuvenation. Two authors who come to mind are Michael Greenwood and William J. Libby. Good luck! David Harry Institute of Forest Genetics deh@s27w007.pswfs.gov USDA Forest Service, Pacific SW Station Phone: 510/559-6439 PO Box 245 FAX: 510/559-6499 Berkeley, CA 94701 My failure to mention other authors is simply due to ignorance and negligence, not to malicious intent. :-) From owner-plants@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!pipex!uunet!yeshua.marcam.com!zip.eecs.umich.edu!umn.edu!msus1.msus.edu!TIGGER.STCLOUD.MSUS.EDU!DEGROOTE From: degroote@TIGGER.STCLOUD.MSUS.EDU Newsgroups: bionet.plants Subject: assessing S-levels in plants Message-ID: <1993Dec7.193940.7093@msus1.msus.edu> Date: 8 Dec 93 01:39:39 GMT Reply-To: degroote@TIGGER.STCLOUD.MSUS.EDU Organization: ST. CLOUD STATE UNIVERSITY, ST. CLOUD, MN Lines: 6 Nntp-Posting-Host: tigger.stcloud.msus.edu Sorry to take up bandwidth. This is for Jonathan who posted a response on this topic. I got delete-happy and lost your e-mail. The analysis of glutathione sounds ideal for this project. If you have the time I would be interested in further details and advice on how to prep samples and carry out the assay. Thanks. David DeGroote From owner-plants@net.bio.net Tue Dec 07 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!warwick!uknet!pipex!uunet!zib-berlin.de!informatik.tu-muenchen.de!lrz-muenchen.de!informatik.uni-muenchen.de!brunner From: brunner@informatik.uni-muenchen.de (Christian Brunner) Newsgroups: bionet.plants Subject: Infos about RAFFLESIACEAE ? Message-ID: Date: 8 Dec 93 15:22:47 GMT Sender: news@informatik.uni-muenchen.de (News System) Organization: Institut fuer Informatik der Universitaet Muenchen Lines: 10 Originator: brunner@skoll.cip.informatik.uni-muenchen.de Hi, I'm looking for informations about RAFFLESIACEAE ? If anyone knows articles about it or books, please drop me some lines of email. Thanks, Christian From owner-plants@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!bcm!cs.utexas.edu!uunet!olivea!hal.com!decwrl!concert!samba.oit.unc.edu!sunSITE!london From: london@sunSITE.unc.edu (Larry London) Newsgroups: alt.sustainable.agriculture,bionet.plants,alt.agriculture.misc Subject: Sci.agriculture CFV Message-ID: <2dl0ms$7cv@samba.oit.unc.edu> Date: 2 Dec 93 15:13:32 GMT Organization: University of North Carolina, Chapel Hill Lines: 99 Xref: biosci alt.sustainable.agriculture:1918 bionet.plants:2220 alt.agriculture.misc:517 NNTP-Posting-Host: calypso.oit.unc.edu ---------- Forwarded message ---------- Date: Mon, 29 Nov 93 13:30:11 EST From: L. Todd Masco To: sustag@beta.tricity.wsu.edu Subject: CFV: sci.agriculture FIRST CALL FOR VOTES (of 2) This is the first of two Call For Votes (CFV) regarding the proposed creation of a new newsgroup. Please read the proposal before voting. Detailed instructions for voting are given below. Unmoderated group sci.agriculture Newsgroups line: sci.agriculture Farming, agriculture and related topics All votes must be received by 23:59:59 UTC, 15 December 1993. This vote is being conducted by a neutral third party. All questions regarding voting and procedure or reports of problems should be mailed to "L. Todd Masco" . All votes should be mailed to vote@clinton.com All questions regarding the proposed new group should be mailed to the proposer, David Bromage . This CFV is being posted to the following newsgroups: news.announce.newgroups news.groups alt.sustainable.agriculture bionet.agroforestry bionet.general sci.environment sci.misc talk.environment And once the CFV has appeared, it will also be mailed to the mailing list: sustag@beta.tricity.wsu.edu CHARTER This newsgroup is intended for the discussion of any topics related to farming and agriculture. Topics for discussion include, but are not limited to: - Land management - Water management - Soils - Erosion (prevention of) - Forestry - Hydroponics - Aeroponics - Aquaculture - Permaculture - Economic aspects - Advice and assistance including personal experience - Announcement of forthcoming courses and conferences - Discussion and criticism of literature VOTING INSTRUCTIONS You should send MAIL (posts to any newsgroup or mail to any other address are invalid) to vote@clinton.com (just replying by MAIL to this message should work). Your mail message's body should contain one and only one of the following exact statements: I vote YES on sci.agriculture I vote NO on sci.agriculture I vote ABSTAIN on sci.agriculture I vote CANCEL on sci.agriculture Anything else may be rejected by the automatic vote counting program. You may ABSTAIN in place of YES or NO - abstain votes do not affect the vote outcome; you may also change your vote later by voting again. Within a few days, the votetaker will respond to received ballots with mail acknowledging your vote. Addresses and votes of all voters will be published in the vote results. If you wish to remove your vote and all indication that you have voted from the final list, send a vote with CANCEL in the place of YES/NO/ABSTAIN. Standard Guidelines for voting apply. One vote per person, no more than one vote per account. 100 more YES votes than NO votes and twice as many YES votes as NO votes are the requirements for group creation. Votes must be tracable to a human: votes from system accounts such as (but not limited to) "news" or "root" will be marked invalid. -- L. Todd Masco This vote is being handled by the System Administrator/Programmer, USENET Volunteer Votetakers. From owner-plants@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!daresbury!zeta.bmc.uu.se!corax.udac.uu.se!sunic!EU.net!howland.reston.ans.net!europa.eng.gtefsd.com!news.umbc.edu!haven.umd.edu!umd5.umd.edu!usenet From: bwilliam@oyster.smcm.edu (Bill Williams) Newsgroups: bionet.plants Subject: Re: Christmas Tree Maintenance? Message-ID: <2e7uao$1p0@umd5.umd.edu> Date: 9 Dec 93 19:29:28 GMT References: <2e7p2e$3kt@chemsci1.es.dupont.com> Organization: St. Mary's College of Maryland Lines: 9 NNTP-Posting-Host: williams.smcm.edu X-Posted-From: InterNews 1.0.1b7@williams.smcm.edu I understand aspirin works well, too, but I don't know the proper dose. I seem to remember an incident where the parents of a Vietnam POW managed to keep "his" Christmas tree in needles for several years with appropriate doses of aspirin. _________________________________ William E. Williams Department of Biology St. Mary's College of Maryland From owner-plants@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!daresbury!zeta.bmc.uu.se!corax.udac.uu.se!sunic!pipex!howland.reston.ans.net!agate!darkstar.UCSC.EDU!cats.ucsc.edu!moria From: moria@cats.ucsc.edu (Orion Wilson) Newsgroups: alt.drugs,talk.politics.drugs,alt.hemp,alt.conspiracy,alt.society.generation-x,alt.wired,sci.med.pharmacy,alt.fan.rush-limbaugh,alt.flame,bionet.plants,alt.psychoactives Subject: Re: HOORAY for Surgeon General Elders!! Message-ID: <2e8703$5jk@darkstar.UCSC.EDU> Date: 9 Dec 93 21:57:23 GMT References: Organization: University of California, Santa Cruz Lines: 6 Xref: biosci alt.drugs:30345 talk.politics.drugs:7350 alt.hemp:1830 alt.conspiracy:20935 alt.society.generation-x:3862 alt.wired:2041 sci.med.pharmacy:807 alt.fan.rush-limbaugh:53608 alt.flame:50443 bionet.plants:2221 NNTP-Posting-Host: am.ucsc.edu X-Newsreader: NN version 6.5.0 #1 (NOV) my my what a factual , draws-logical-conclusions, and thoughtful provoking post, dan. cheers for you. -- AND AS I RAISE MY VOICE I N T H EhVeAaLvLeEnYlOFyD E A T H - iwill fearno evil AND THO I RAISE my child IN THE VALLEY OF DEATH ... From owner-plants@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!daresbury!zeta.bmc.uu.se!corax.udac.uu.se!sunic!EU.net!howland.reston.ans.net!europa.eng.gtefsd.com!emory!news-feed-2.peachnet.edu!concert!corpgate!news.utdallas.edu!greer From: greer@utdallas.edu (Dale M. Greer) Newsgroups: alt.drugs,talk.politics.drugs,alt.psychoactives,alt.conspiracy,alt.society.generation-x,alt.wired,sci.med.pharmacy,alt.fan.rush-limbaugh,alt.flame,bionet.plants,ca.politics Subject: Re: HOORAY for Surgeon General Elders!! Message-ID: <2e7vsm$9dr@news.utdallas.edu> Date: 9 Dec 93 19:56:06 GMT References: <2e7c4h$pmn@darkstar.UCSC.EDU> Followup-To: alt.drugs,talk.politics.drugs,alt.psychoactives,alt.conspiracy,alt.society.generation-x,alt.wired,sci.med.pharmacy,alt.fan.rush-limbaugh,alt.flame,bionet.plants,ca.politics Organization: The University of Texas at Dallas Lines: 43 Xref: biosci alt.drugs:30333 talk.politics.drugs:7344 alt.conspiracy:20929 alt.society.generation-x:3856 alt.wired:2037 sci.med.pharmacy:803 alt.fan.rush-limbaugh:53586 alt.flame:50432 bionet.plants:2213 ca.politics:12898 NNTP-Posting-Host: csssun10.utdallas.edu X-Newsreader: TIN [version 1.2 PL2] The Pinhead (zippy@hairball.ecst.csuchico.edu) wrote: > In article <2e7c4h$pmn@darkstar.UCSC.EDU> moksha@cats.ucsc.edu (Matthew Michael Brenner) writes: > I urge everyone reading this who has a scintilla of grey-matter in their > heads: write the prez. and tell him to listen to his Surgeon General: > PRESIDENT@WHITEHOUSE.GOV > Not only doesn't the president read the email, his flunkies don't even > answer it with snail mail like they claimed they would. I dare say > that no one has received a reply. If you have, EFF and myself would > like to know. I got an automated response, not a reply to the content of my e-mail, just an automated "thank you, and isn't this e-mail thing just great and we're going to be continually expanding services, etc." As for actually reading it, no I doubt that anyone reads much of it, but I do know that, in general, politicians' assistants will read enough to discern which side of the fence the citizen is on, as long as it isn't too hard to figure it out. So if you do send something, make it short and to the point, with your position stated in CAPS, like so I SUPPORT Surgeon General Joycelyn Elder's suggestion to study the legalization of drugs. I SUPPORT the legalization of marijuana. or, as the case may be, I DO NOT SUPPORT Surgeon General... You get the picture. And, if you like, put "I WILL NOT VOTE FOR YOU if..." after you've stated your main viewpoint. But don't put more than a few sentences unless you just feel like getting it out of your system. > Ronald Cole E-mail: ronald@netcom.com > "The Bill Of Rights -- Void Where Prohibited By Law" Indeed. -- Dale Greer, greer@utdallas.edu From owner-plants@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!daresbury!zeta.bmc.uu.se!corax.udac.uu.se!sunic!EU.net!howland.reston.ans.net!math.ohio-state.edu!magnus.acs.ohio-state.edu!cis.ohio-state.edu!news.sei.cmu.edu!bb3.andrew.cmu.edu!andrew.cmu.edu!dg5a+ From: dg5a+@andrew.cmu.edu (Dan S Goldwasser) Newsgroups: alt.drugs,talk.politics.drugs,alt.hemp,alt.conspiracy,alt.society.generation-x,alt.wired,sci.med.pharmacy,alt.fan.rush-limbaugh,alt.flame,bionet.plants,alt.psychoactives Subject: Re: HOORAY for Surgeon General Elders!! Message-ID: Date: 9 Dec 93 19:33:10 GMT Organization: Freshman, MCS general, Carnegie Mellon, Pittsburgh, PA Lines: 41 Xref: biosci alt.drugs:30331 talk.politics.drugs:7343 alt.hemp:1827 alt.conspiracy:20928 alt.society.generation-x:3855 alt.wired:2036 sci.med.pharmacy:802 alt.fan.rush-limbaugh:53585 alt.flame:50431 bionet.plants:2212 NNTP-Posting-Host: po5.andrew.cmu.edu In-Reply-To: <2e7c4h$pmn@darkstar.UCSC.EDU> Excerpts from netnews.alt.fan.rush-limbaugh: 9-Dec-93 HOORAY for Surgeon General .. by Matthew M. Brenner@cats. > It's so heartening to see it coming from such a respectable person as > Dr. Elders. Now, what's so surprising is the Whitehouse's reaction: > something like "the Surgeon General is entitled to her opinion but > Mr. Clinton is opposed to the idea and doesn't want to study it." > > Doesn't want to even study it? That's all Elders called for, mind you. > She said that based on two other countries experience of legalizing illicit > drugs, it would be worth studying here. She said that it seemed to reduce > violent crime by taking out the profit, and that the number of users did > not go up. Wow! Could Jocelyn Elders be the first public official not > in the pockets of the MEDELLIN drug cartel, the Mafia, or the alcohol & > tobacco companies? > Oh, please!! Jocelyn Elders is certifiably insane! She said something on the lines of, "We need safer guns, and safer bullets. And since we have car licenses we should have Gun licenses." How ridiculous is THAT?? She wears a military-type uniform (because, after all, she IS the Surgeon GENERAL), and talks about how we need gun licenses?? Where has she been all these years? Oh yeah. Arkansas. Well, maybe they don't have gun licenses there, but most of the country does. Isn't "safer bullets" an oxymoron? What should the bullet do? Hold out a sign that says "BANG"? Please! What's comes after she teaches our kindergarten children about sex? Starting a safe-shooting campaign? "Teenagers are going to shoot anyway. So, let's GIVE them the guns, and teach them how to use them......beginning with the pre-schools." J. Elders churns my stomach. Dan --------------------------------------------- It's still the economy, and he's still stupid. From owner-plants@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!MVS.CSO.NIU.EDU!T80SMS1 From: T80SMS1@MVS.CSO.NIU.EDU Newsgroups: bionet.plants Subject: Re: Sassafra leaf morphology Message-ID: <9312091735.AA07937@net.bio.net> Date: 9 Dec 93 17:33:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 40 Sorry to bother the list with this, but the original message did not have a return address. ______________________________________________________ Katie, AH, the magic phrase "phenotypic plasticity". As a start I (humbly) suggest my article in the latest Ann. Rev. Ecol. Syst. on same. My reaction to your problem is that you are dealing with a prediction problem. The organism (in this case the bud) needs to "decide" what its phenotype will be based on data 1 year prior to phenotypic expression. Given the low, albeit still postive, correlation between those environments, the best strategy might be to use better cues such as branch age or bud position on the tree. That is, if the average bud on a 5 year old branch has a 10% light level, then the optimal evolutionary strategy is to have a morphology that is "best" for that light level. If there are costs associated with trying to fine-tune, such a strategy is optimal. See papers by Gavrilets & me and by Van Tienderen (referenced in ARES article). I would suggest that you try an experiment. Change the light received by different buds and see if you can change the leaf morphology. That might tell you what the proximal cue is which might suggest how evolution is proceeding. Of course, with this 1-year potential lag patience is a virtue :-). If you are interested I can send some reprints. This sounds like a facinating problem and I am always interested in plasticity problems. Sam *+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+* * Samuel M. Scheiner + * * Department of Biological Sciences + In a bureaucracy it * * Northern Illinois University + * * DeKalb, Illinois 60115 + is much easier to get * * Phone: (815) 753-7847 + * * Fax: (815) 753-0461 + forgiveness than * * Bitnet: t80sms1@niu.bitnet + * * Internet: t80sms1@mvs.cso.niu.edu + permission. * *+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+*+* From owner-plants@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!daresbury!zeta.bmc.uu.se!corax.udac.uu.se!sunic!EU.net!howland.reston.ans.net!europa.eng.gtefsd.com!emory!cs.utk.edu!cs.utk.edu!usenet From: farmer@cs.utk.edu (SUSAN FARMER) Newsgroups: bionet.plants Subject: Re: Christmas Tree Maintenance? Message-ID: Date: 9 Dec 93 19:12:20 GMT References: <2e7p2e$3kt@chemsci1.es.dupont.com> Organization: Computer Science Alumnus ... Department of Botany Lines: 33 NNTP-Posting-Host: utkcs2.cs.utk.edu In article <2e7p2e$3kt@chemsci1.es.dupont.com> Dayton Meyer writes: >After buying our cut Christmas tree, the salesman advised my roommate to >use very dilute sugar water in the reservoir in order to keep the tree >thriving throughout the long holiday season. I've heard of recipes like >this before, but never kept record of them. > >Does anybody have any recipes like this, assuming they have positive >efficacy? > >Thanks, > >Dayton Meyer >Wilmington, DE > >meyerdt@chemsci1.es.dupont.com Well, I can't speak for Christmas Trees, but my mother-in-law is a florist and *especially* for roses, she advises her customers to use 50% water and 50% sprite or 7-up (NOT sugar-free). The acid and the sugar are supposed to be beneficial for the cut flowers. However, with the addition of the sugar, you have to be absolutely religious about changing the water frequently or you get nasty overgrowth of molds and bacteria which are none to healthy for the plants. It sort of follows that this might work for Christmas trees, but I don't know. Susan -- Susan B. Farmer farmer@cs.utk.edu Lady Jerusha Kilgore I can't even find time to clean house and you want me to come up with a .sig? B.A. in Computer Science ... Graduate Student in Botany Talk to me about your Trillium species! From owner-plants@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!am.abru.cg.com!DELANEYT From: DELANEYT@am.abru.cg.com (DELANEYT 919-541-8577) Newsgroups: bionet.plants Subject: Reply to: aniline blue and pollen tubes Message-ID: <9017281009121993.A25317.YOMAMA.117C4A9C0F00*@abru.cg.com> Date: 9 Dec 93 20:28:17 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 35 Dear Alastair, We are examining callose deposition using 0.01% aniline blue in M/15 K2HPO4 (ph ~9.5), modeled after Eschrich and Currier (1964) Stain Technol. 39: 303-307. The solution tends to get quite pale after an overnight period. The solution is stored under a foil wrap to prevent light degradation. Usually I clear leaves with lactophenol (10 mls lactic acid, 10 mls phenol, 10 mls glycerol, 10 mls water) vacuum infiltation and heating (100 degrees C) for a few minutes (adapted from Keogh et al (1980) Trans Br. Mycol. Soc. 74: 329-333). After overnight, the tissue is transferred into a solution of chloral hydrate (25 g plus 10 mls water), and changed once or twice until the tissue is like glass. A brief treatment with the aniline blue solution (30-60 min) will stain callose tissues (e.g. vascular tissue, pollen tubes, plant defense responses (HRs)). You may want to take the tissue down to 100 percent aqueous in a few steps from the chloral hydrate, if you find plasmolysis to occur in your tissue. Following staining, transfer tissue to stain-free water to visualize under UV or blue excitation (390-440 nM). There is an alternate recipe for a one step fix/decolorize solution listed below. Its lower viscosity helps for infiltrating leaves, although the tissues don't seem to get quite as clear. Using either solution, we don't usually have overstaining, and the fluorescent emission is quite bright. Alternate solution (less viscous) 16.7 mls ETOH 8.3 mls chloroform 8.2 mls phenol 6.9 mls lactic acid 25 g chloral hydrate Hope this helps, Terry Delaney Ciba Agric Biotech delaneyt@am.abru.cg.com From owner-plants@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!warwick!pipex!howland.reston.ans.net!europa.eng.gtefsd.com!darwin.sura.net!gatekeeper.es.dupont.com!chemsci1.es.dupont.com!chem129.es.dupont.com!meyerdt From: meyerdt@chemsci1.es.dupont.com (Dayton Meyer) Newsgroups: bionet.plants Subject: Christmas Tree Maintenance? Message-ID: <2e7p2e$3kt@chemsci1.es.dupont.com> Date: 9 Dec 93 17:59:42 GMT Organization: DuPont Merck Pharmaceutical Company Lines: 14 NNTP-Posting-Host: chem129 X-UserAgent: Nuntius v1.1.1d17 X-XXMessage-ID: X-XXDate: Thu, 9 Dec 93 05:01:57 GMT After buying our cut Christmas tree, the salesman advised my roommate to use very dilute sugar water in the reservoir in order to keep the tree thriving throughout the long holiday season. I've heard of recipes like this before, but never kept record of them. Does anybody have any recipes like this, assuming they have positive efficacy? Thanks, Dayton Meyer Wilmington, DE meyerdt@chemsci1.es.dupont.com From owner-plants@net.bio.net Wed Dec 08 22:00:00 1993 Path: biosci!daresbury!zeta.bmc.uu.se!corax.udac.uu.se!sunic!pipex!uunet!yeshua.marcam.com!charnel!zippy From: zippy@hairball.ecst.csuchico.edu (The Pinhead) Newsgroups: alt.drugs,talk.politics.drugs,alt.psychoactives,alt.conspiracy,alt.society.generation-x,alt.wired,sci.med.pharmacy,alt.fan.rush-limbaugh,alt.flame,bionet.plants,ca.politics Subject: Re: HOORAY for Surgeon General Elders!! Message-ID: Date: 9 Dec 93 17:14:09 GMT References: <2e7c4h$pmn@darkstar.UCSC.EDU> Organization: California State University, Chico Lines: 32 Xref: biosci alt.drugs:30316 talk.politics.drugs:7342 alt.conspiracy:20921 alt.society.generation-x:3848 alt.wired:2035 sci.med.pharmacy:801 alt.fan.rush-limbaugh:53550 alt.flame:50426 bionet.plants:2207 ca.politics:12896 NNTP-Posting-Host: hairball.ecst.csuchico.edu In-reply-to: moksha@cats.ucsc.edu's message of 09 Dec 1993 06:18:57 PST In article <2e7c4h$pmn@darkstar.UCSC.EDU> moksha@cats.ucsc.edu (Matthew Michael Brenner) writes: To me this is the first truly original idea in American politics in my lifetime. It's so heartening to see it coming from such a respectable person as Dr. Elders. Now, what's so surprising is the Whitehouse's reaction: something like "the Surgeon General is entitled to her opinion but Mr. Clinton is opposed to the idea and doesn't want to study it." Doesn't want to even study it? That's all Elders called for, mind you. She said that based on two other countries experience of legalizing illicit drugs, it would be worth studying here. She said that it seemed to reduce violent crime by taking out the profit, and that the number of users did not go up. Wow! Could Jocelyn Elders be the first public official not in the pockets of the MEDELLIN drug cartel, the Mafia, or the alcohol & tobacco companies? Agreed. Perhaps she listens to Dr. Dean Edell? I urge ever