From owner-proteins@net.bio.net Mon Mar 01 22:00:00 1993 Path: biosci!agate!howland.reston.ans.net!gatech!willis1.cis.uab.edu!uabdpo.dpo.uab.edu!gila005 Newsgroups: bionet.general,bionet.molbio.proteins,bionet.molbio.methds-reagnts,sci.med Subject: Need source for KLH (pharmaceutical grade) Message-ID: <1993Mar2.231428.5752@cis.uab.edu> Date: 2 Mar 93 23:14:28 GMT Sender: root@cis.uab.edu (Operator) Organization: Univ. of Alabama at Birmingham Lines: 9 Xref: biosci bionet.general:4213 bionet.molbio.proteins:549 bionet.molbio.methds-reagnts:4523 sci.med:9724 I need a source for KLH to be used for injection for testing for delayed type hypersensitivity. Does anyone have a source for this reagent. Thanks in advance. Please respond by E-mail to Steve Holland gila005@uabdpo.dpo.uab.edu From owner-proteins@net.bio.net Mon Mar 01 22:00:00 1993 Path: biosci!rocbi.DNET.roche.com!dorand From: dorand@rocbi.DNET.roche.com ("Daniel M. Doran, PRTC, phone 88270") Newsgroups: bionet.molbio.proteins Subject: RE: Searching a sequence in Brookhaven Protein Data Bank... Message-ID: <9303021116.AA27495@mailgate.roche.com> Date: 2 Mar 93 11:16:33 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 23 > I am trying to search for five or more residues amino acid on all > proteins that the structures have been found. The results then can prove > that "one sequence one structure" is correct or not... That question was looked into years ago. See W. Kabsch and C. Sander in PNAS 81:1075 (1984) I.A. Wilson, et al. in PNAS 82: 5255 (1985) The short answer is that identical sequences can can assume different secondary structures. Dan. ------------------------------------------------------------------------------ Daniel M. Doran, Ph.D. InterNet: DoranD@Rocbi.Dnet.Roche.Com Pharmaceutical Research - Phone: +41-61-688-8270 New Technologies +41-61-688-7080 F.Hoffmann-La Roche Ltd. Fax: +41-61-688-1745 CH-4002 Basel, Switzerland ------------------------------------------------------------------------------ From owner-proteins@net.bio.net Wed Mar 03 22:00:00 1993 Path: biosci!uwm.edu!zaphod.mps.ohio-state.edu!usc!sol.ctr.columbia.edu!ira.uka.de!scsing.switch.ch!sicsun!Ruud From: Ruud hovius Newsgroups: bionet.molbio.proteins Subject: tritium scanners Keywords: tritium, detection Message-ID: <4896@sicsun.epfl.ch> Date: 4 Mar 93 18:52:11 GMT Sender: news@sicsun.epfl.ch Reply-To: hovius@icphp1.epfl.ch Organization: EPFL Lines: 21 Hai netters, I have a tritium labeled protein in (dried) SDS-PAGE gels and I want to determine where and how much radioactivity is present in the gels. I tried slicing up the gels and dissolving them with H2O2, but that is very time consuming. Autoradiography takes weeks; perhaps anyone has a clue how to make this more sensitive ? What seems to work is a gas flow proportional counter from Berthold. Unluckily the very sensitive storage cristals used in the phosphoimager of Molecular Dynamics is not sensitive for the radiation of tritium. The crystals of Fuij, I was told, can only be used once and are rather expensive. Does anybody know of a sensitive and reliable method to quantitatively determine radioactivity in gels ? Please let me know . All hints are appriciated ! Ruud. From owner-proteins@net.bio.net Sun Mar 07 22:00:00 1993 Path: biosci!agate!spool.mu.edu!uwm.edu!linac!uchinews!quads!kb13 From: kb13@quads.uchicago.edu (kristin black) Newsgroups: bionet.molbio.proteins Subject: repeating 5 amino acid motifs in proteins Message-ID: <1993Mar8.204225.17896@midway.uchicago.edu> Date: 8 Mar 93 20:42:25 GMT Sender: news@uchinews.uchicago.edu (News System) Reply-To: kb13@midway.uchicago.edu Organization: University of Chicago Lines: 9 I have sequenced a gene which is probably involved in the localization of glycolipids in a cyanobacterium. It has very little homology to other sequences in Genbank but it does have an interesting repeated amino acid motif. One segment of the protein has thirty-six degenerate repeats of five amino acids including a mostly conserved leucine position. Does anyone have any ideas about the potential function(s) of such repeats? Kristin Black kb13@midway.uchicago.edu University of Chicago From owner-proteins@net.bio.net Mon Mar 08 22:00:00 1993 Path: biosci!agate!spool.mu.edu!uwm.edu!caen!batcomputer!cornell!uw-beaver!news.tek.com!psgrain!ee.und.ac.za!ford.ee.up.ac.za!hippo!ucthpx!uctvax.uct.ac.za!blatch From: blatch@uctvax.uct.ac.za Newsgroups: bionet.molbio.proteins Subject: problem with elution from hydrophobic column Message-ID: <1993Mar9.140331.203720@uctvax.uct.ac.za> Date: 9 Mar 93 12:03:31 GMT Organization: University of Cape Town Lines: 12 PROBLEM WITH ELUTION CONDITIONS FOR A HYDROPHOBIC COLUMN: We are trying to purify a protein using a hydrophobic material called FRACTOGEL TSK BUTYL-650 (M) produced by Merck. The matrix consists of butyl residues linked by an ether link to a polyvinyl support matrix. Our protein sample will stick to the matrix at approx 0.8 M ammonium sulphate but under no circumstances will any proteins elute from the matrix even if we use distilled water. Note our protein sample will not stick to the matrix in the absence of salt. So we are dealing with what seems to be an irreversible binding process. What are we doing wrong? Must we just change to a completely different hydrophobic moiety? Any suggestions will be greatly appreciated. Please send suggestions to me or Neil@micro.uct.ac.za From owner-proteins@net.bio.net Tue Mar 09 22:00:00 1993 Path: biosci!agate!spool.mu.edu!sdd.hp.com!saimiri.primate.wisc.edu!usenet.coe.montana.edu!decwrl!uunet!cis.ohio-state.edu!news.sei.cmu.edu!fs7.ece.cmu.edu!pellns.alleg.edu!news From: sesslea@carr58.alleg.edu (Anna Sessler) Newsgroups: bionet.molbio.proteins Subject: protein detection Message-ID: <1993Mar9.212937.2603@pellns.alleg.edu> Date: 9 Mar 93 21:29:37 GMT Sender: news@pellns.alleg.edu Distribution: World Organization: Allegheny College Lines: 7 Does anybody know what the most sensitive, yet fairly cheap method of protein detection in gel electrophoresis is? Thank you. Anna email: sesslea@alleg.edu From owner-proteins@net.bio.net Tue Mar 09 22:00:00 1993 Path: biosci!agate!spool.mu.edu!hri.com!noc.near.net!uunet!utcsri!utgpu!japhale From: japhale@gpu.utcc.utoronto.ca (J. Aphale) Newsgroups: bionet.xtallography,bionet.software,bionet.general,bionet.molbio.proteins Subject: Molecule Modelling Software Message-ID: Date: 10 Mar 93 17:04:28 GMT Followup-To: bionet.xtallography,bionet.software,bionet.followup,bionet.molbio.proteins Distribution: na Organization: UTCC Public Access Lines: 17 Xref: biosci bionet.xtallography:185 bionet.software:4449 bionet.general:4271 bionet.molbio.proteins:555 Hello Netters: I am looking for a "Molecule Modelling" program that can be run on an IBM compatible PC. Specifically, I am planning to look into ligand-receptor interactions and ultimately want to examine (via such a program) effect of site-specific mutations on such interactions. Alternately, is there such a program available on any of the mainframes that can be accessed via Internet? Any and all suggestions will be appreciated. My e-mail address is japhale@gpu.utcc.utoronto.ca. Thanx in advance. Jayant. From owner-proteins@net.bio.net Tue Mar 09 22:00:00 1993 Path: biosci!agate!howland.reston.ans.net!bogus.sura.net!darwin.sura.net!news.Vanderbilt.Edu!NewsWatcher!user From: bai@macpost.vanderbilt.edu (Feng Bai) Newsgroups: bionet.molbio.proteins Subject: How to isolate plant protein Message-ID: Date: 10 Mar 93 16:55:27 GMT Sender: news@news.vanderbilt.edu Followup-To: bionet.molbio.proteins Organization: Department of Pathology, Vanderbilt Lines: 4 Nntp-Posting-Host: 129.59.16.56 Whoever know how to isolate proteins from plant cells, pleasecontact me or send me a good protocol. In addition, if you know how to run two-dimesion gel for plant proteins, please tell me. From owner-proteins@net.bio.net Thu Mar 11 22:00:00 1993 Path: biosci!agate!howland.reston.ans.net!newsserver.jvnc.net!netnews.upenn.edu!duong From: duong@chestnut.chem.upenn.edu (Duc Duong) Newsgroups: bionet.software,bionet.xtallography,bionet.molbio.proteins Subject: Root mean square distance between two known structures? Message-ID: <113595@netnews.upenn.edu> Date: 12 Mar 93 18:32:25 GMT Sender: news@netnews.upenn.edu Followup-To: bionet.software Organization: NMR Biochemistry Graduate Research Lab Lines: 6 Xref: biosci bionet.software:4466 bionet.xtallography:194 bionet.molbio.proteins:556 Nntp-Posting-Host: chestnut.chem.upenn.edu hi.. does any one know a software (PD or commercial) that will compare 3D structures of two proteins by measuring minimum possible root mean square bwetween equivalent C alpha positions? From owner-proteins@net.bio.net Thu Mar 11 22:00:00 1993 Path: biosci!agate!howland.reston.ans.net!zaphod.mps.ohio-state.edu!magnus.acs.ohio-state.edu!usenet.ins.cwru.edu!cleveland.Freenet.Edu!bl275 From: bl275@cleveland.Freenet.Edu (Dan Diaz) Newsgroups: bionet.molbio.proteins Subject: Re: problem with elution from hydrophobic column Message-ID: <1nrm4nINNqn2@usenet.INS.CWRU.Edu> Date: 13 Mar 93 03:58:15 GMT References: <1993Mar9.140331.203720@uctvax.uct.ac.za> Reply-To: bl275@cleveland.Freenet.Edu (Dan Diaz) Organization: Case Western Reserve University, Cleveland, OH (USA) Lines: 27 NNTP-Posting-Host: hela.ins.cwru.edu In a previous article, blatch@uctvax.uct.ac.za () says: >PROBLEM WITH ELUTION CONDITIONS FOR A HYDROPHOBIC COLUMN: > >We are trying to purify a protein using a hydrophobic material called FRACTOGEL >TSK BUTYL-650 (M) produced by Merck. The matrix consists of butyl residues >linked by an ether link to a polyvinyl support matrix. Our protein sample will >stick to the matrix at approx 0.8 M ammonium sulphate but under no >circumstances will any proteins elute from the matrix even if we use distilled >water. Note our protein sample will not stick to the matrix in the absence of >salt. So we are dealing with what seems to be an irreversible binding process. >What are we doing wrong? Must we just change to a completely different >hydrophobic moiety? Any suggestions will be greatly appreciated. Please send >suggestions to me or Neil@micro.uct.ac.za > > > > Suggestions: 1. Add ethylene glycol to you eluent 2. Lower the temperature (weakens Hphobic interactions) 3. Use a less hydrophobic resin, like phenyl -- Dizzy Dan Department of Molecular Genetics and Curried Sauces Albert Einstein College of Medicine, Da Bronx, New York From owner-proteins@net.bio.net Sat Mar 13 22:00:00 1993 Path: biosci!agate!ames!decwrl!netcomsv!netcom.com!zap From: zap@netcom.com (Paul Eastham) Newsgroups: bionet.molbio.proteins Subject: Tyrosine concentration in casein? Message-ID: <1993Mar14.193643.9492@netcom.com> Date: 14 Mar 93 19:36:43 GMT Organization: Netcom Lines: 13 I'm looking for the tyrosine concentration present in casein for a biochemistry experiment at San Jose State. We are supposed to compare theoretical yield to our yield from the experiment. The problem is I haven't been able to find the theoretical yield in any of the sources I have available (CRC, biochem. texts, and analytical chem. texts.) Thanks in advance for any info/poiers! Post or reply to zap@netcom.com. -- Paul Eastham Home: zap@netcom.com -- Los Altos, CA Work: paul@cerberus.arc.nasa.gov -- Ames Research Center, Mountain View, CA Play Vincent's Hollow LPMud: mud.iastate.edu 1992 "There are no facts, only interpretations." -- Nietzsche From owner-proteins@net.bio.net Sat Mar 13 22:00:00 1993 Path: biosci!agate!howland.reston.ans.net!bogus.sura.net!darwin.sura.net!sgiblab!munnari.oz.au!comp.vuw.ac.nz!canterbury.ac.nz!otago.ac.nz!bioc07 From: bioc07@otago.ac.nz Newsgroups: bionet.molbio.proteins Subject: Re: problem with elution from hydrophobic column Message-ID: <1993Mar14.231356.874@otago.ac.nz> Date: 14 Mar 93 10:13:56 GMT References: <1993Mar9.140331.203720@uctvax.uct.ac.za> Organization: University of Otago, Dunedin, New Zealand Lines: 28 In article <1993Mar9.140331.203720@uctvax.uct.ac.za>, blatch@uctvax.uct.ac.za writes: > PROBLEM WITH ELUTION CONDITIONS FOR A HYDROPHOBIC COLUMN: > . . . > hydrophobic moiety? Any suggestions will be greatly appreciated. > Please send suggestions to me or Neil@micro.uct.ac.za > > > You might try using a non-ionic detergent such as Tween 20 to elute your material. I have had success with this on Pharmacia Phenyl-superose columns. The inclusion of 0.02% Tween 20 in the elution buffer has both given me better yields of some proteins and removed others that otherwise needed acetonitrile to get them off the column. The elution position will be somewhat advanced but this need not be a problem. -- Craig Marshall bioc07@otago.ac.nz Biochemistry Department University of Otago Phone 64 3 479 7849 P.O. Box 56 Fax 64 3 479 7866 Dunedin, New Zealand From owner-proteins@net.bio.net Sat Mar 13 22:00:00 1993 Path: biosci!daresbury!buzz.bmc.uu.se!corax.udac.uu.se!sunic!pipex!warwick!uknet!pavo.csi.cam.ac.uk!nntp-serv.cam.ac.uk!seb1005 From: seb1005@bio.cam.ac.uk (Steven Brenner) Newsgroups: bionet.software,bionet.molbio.proteins Subject: Making multimers from monomers and PDB translation data Message-ID: Date: 15 Mar 93 00:13:52 GMT Sender: news@infodev.cam.ac.uk (USENET news) Followup-To: bionet.software Distribution: bionet Organization: U of Cambridge, England Lines: 36 Xref: biosci bionet.software:4478 bionet.molbio.proteins:560 Nntp-Posting-Host: mbfs.bio.cam.ac.uk The Protein Databank files frequently contain the structure only a single chain of a homo-multimer but provide records describing how to translate the coordinates for that monomer in order to produce the entire protein. For much work (for example, on solvent accessibility) it is essential to work with the entire protein rather than with just the monomeric chain. I imagine it would be simple enough to write a little program to do the translations to generate the complete protein, but it would be even simpler to use one that has already been written. Does anyone know of such a program? Two caveats: 1) I have been told that many of the translation records are systematically incorrect as a result of a bug in an early crystallographic program. Is this true? If so, is there a program (or at least an algorithm) to convert the erroneous translation records to correct ones? 2) I would like to be able to automate the process, since I will be analyzing all of the proteins in the PDB. Therefore, I would rather use a program which can be called from the command-line than an interactive one. However, information about any programs that will do the job would be appreciated. Thanks in advance -Steven -- Steven E. Brenner | Internet seb1005@mbfs.bio.cam.ac.uk Department of Biochemistry | JANET seb1005@uk.ac.cam.bio.mbfs University of Cambridge | Laboratory +44 223 333671 Tennis Court Road | Home +44 223 314964 Cambridge CB2 1QW, UK | Lab Fax +44 223 333345 From owner-proteins@net.bio.net Sun Mar 14 22:00:00 1993 Path: biosci!agate!howland.reston.ans.net!zaphod.mps.ohio-state.edu!saimiri.primate.wisc.edu!zazen!news From: nmrdb@vms.macc.wisc.edu (BEVERLY SEAVEY) Newsgroups: bionet.molbio.proteins Subject: What is the actions of NP-5, sapecin, etc? Message-ID: <1993Mar15.213445.16752@macc.wisc.edu> Date: 15 Mar 93 23:32:35 GMT Sender: news@macc.wisc.edu (USENET News System) Organization: University of Wisconsin Academic Computing Center Lines: 3 Can anyone tell me what the underlying mechanism of the toxins in the antimicrobial family of defensins, sapecins, NP-5 is? From owner-proteins@net.bio.net Sun Mar 14 22:00:00 1993 Path: biosci!NBRF.GEORGETOWN.EDU!POSTMASTER From: POSTMASTER@NBRF.GEORGETOWN.EDU Newsgroups: bionet.molbio.proteins Subject: Re: Making multimers from monomers and PDB translation data Message-ID: <01GVU4RCYK1U9I48I0@NBRF.Georgetown.Edu> Date: 15 Mar 93 16:29:09 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 35 In message <9303150316.AA23839@net.bio.net> Steven Brenner (seb1005@bio.cam.ac.uk) asks > The Protein Databank files frequently contain the structure only a > single chain of a homo-multimer but provide records describing how to > translate the coordinates for that monomer in order to produce the > entire protein. ... > Does anyone know of such a program? The translation-rotation matrices in the MATRX records are very easy to work with. They are usable by all the commercial molecular modeling programs I know of. A few lines of code from something like "Numerical Recipes in [C/FORTRAN]: The Art of Scientific Computing", W.H. Press, et al., Cambridge University Press, 1988, should give you what you need. He continues: > I have been told that many of the translation records are > systematically incorrect as a result of a bug in an early > crystallographic program. Is this true? If so, is there a program > (or at least an algorithm) to convert the erroneous translation > records to correct ones? I think a more appropriate forum for this question would be the group PROTEIN-CRYSTALLOGRAPHY at xtal-log@net.bio.net and the corresponding European network address. In that group your question could be addressed by a member of the PDB staff and other crystallographers. ------------------------------------------------------------------------ Dr. John S. Garavelli Database Coordinator Protein Information Resource National Biomedical Research Foundation Washington, DC 20007 POSTMAST@GUNBRF.BITNET POSTMASTER@NBRF.GEORGETOWN.EDU From owner-proteins@net.bio.net Mon Mar 15 22:00:00 1993 Path: biosci!agate!howland.reston.ans.net!newsserver.jvnc.net!jvnc.net!tigger.jvnc.net!sandy From: sandy@nmr1.pt.cyanamid.COM (Sandy Silverman) Newsgroups: bionet.molbio.proteins Subject: Re: Tyrosine concentration in casein? Message-ID: Date: 16 Mar 93 20:42:14 GMT References: <1993Mar14.193643.9492@netcom.com> Sender: news@tigger.jvnc.net (Zee News Genie) Organization: American Cyanamid Company Lines: 5 In-Reply-To: zap@netcom.com's message of Sun, 14 Mar 1993 19:36:43 GMT Nntp-Posting-Host: nmr1.pt.cyanamid.com According to Difco, Bacto casamino acids is 2.0% tyrosine. Hope this helps. -- Sanford Silverman >Opinions expressed here are my own< American Cyanamid sandy@pt.cyanamid.com, silvermans@pt.cyanamid.com "Yeast is Best" From owner-proteins@net.bio.net Tue Mar 16 22:00:00 1993 Path: biosci!lhc!darwin.sura.net!news_feed_1.peachnet.edu!gatech!howland.reston.ans.net!newsserver.jvnc.net!princeton!phoenix.Princeton.EDU!angelo From: angelo@phoenix.Princeton.EDU (Angelo Gunasekera) Newsgroups: bionet.molbio.proteins Subject: help HOMOLOGY Message-ID: <1993Mar16.050634.10834@Princeton.EDU> Date: 16 Mar 93 05:06:34 GMT Sender: news@Princeton.EDU (USENET News System) Distribution: bionet.molbio.methds, bionet.molbio.proteins Organization: Princeton University Lines: 30 Originator: news@nimaster Nntp-Posting-Host: phoenix.princeton.edu Newsgroups: bionet.molbio.proteins Subject: INSIGHT - HOMOLOGY PROGRAM Summary: Expires: Sender: Followup-To: Distribution: bionet.molbio.methds, bionet.molbio.proteins Organization: Princeton University Keywords: I am trying to build up a good working model for a single chain antibody (variable regions of the light and heavy linked by a peptide linker) using the HOMOLOGY program within INSIGHT (Biosym). I am able to transfer SCR (struc. conserved region) coordinates the sequence of my interest. My question is that whether its possible to truncate the sequnce of an existing pdb file to be displayed as a truncated protein? (for eg. even if I use the display command to show only the part of the protein on screen, I always get the full protein sequence information on screen when I invoke the extract sequnce command) One way to get around that problem I think is to edit and delete the unnecessary sequnce coordinates in the pdb file before displaying it on screen. Does that make sense or is there any easy ways of doing the same thing. Since i don't know much about editing pdb files, can someone out there help me doing it. I am using homology program within Insight 2.0 in a silicon graphic work station. Thanks in advance Angelo From owner-proteins@net.bio.net Tue Mar 16 22:00:00 1993 Path: biosci!daresbury!daresbury!news From: HABERMANN@AIMP.UNA.AC.AT Newsgroups: bionet.molbio.proteins Subject: Re: help HOMOLOGY Message-ID: <1993Mar17.131215.4216@gserv1.dl.ac.uk> Date: 17 Mar 93 14:09:00 GMT Sender: list-admin@daresbury.ac.uk Distribution: bionet Lines: 35 Original-To: proteins@uk.ac.daresbury X-Vms-To: BITNET"proteins@daresbury.ac.uk" Angelo writes: >I am trying to build up a good working model for a single chain antibody >(variable regions of the light and heavy linked by a peptide linker) >using the HOMOLOGY program within INSIGHT (Biosym). I am able to >transfer SCR (struc. conserved region) coordinates the sequence of my >interest. My question is that whether its possible to truncate the >sequnce of an existing pdb file to be displayed as a truncated protein? >(for eg. even if I use the display command to show only the part of the >protein on screen, I always get the full protein sequence information on >screen when I invoke the extract sequnce command) >One way to get around that problem I think is to edit and delete the >unnecessary sequnce coordinates in the pdb file before displaying it on >screen. Does that make sense or is there any easy ways of doing the >same thing. Since i don't know much about editing pdb files, can >someone out there help me doing it. >I am using homology program within Insight 2.0 in a silicon graphic work >station. >Thanks in advance >Angelo I quite don't understand, why you have problems with the whole sequence being displayed. Once you have transferred the SCR's of the homologous protein to your own sequence, the pdb_file isn't with the whole coordinate set isn't of great interest any more. apart from that, the variable, non_homologous regions are not really in the way. anyhow, to answer your question: it doesn't help to just NOT DISPLAY the regions you don't need. even if you save them as not displayed, they will always be present in the file. what you could do is to delete them with the RESIDUE_DELETE command in the BIOPOLYMER module, alternatively, you could simply edit the pdb_file and cut out the regions, that do not interest you. good luck bianca habermann@aimp.una.ac.at From owner-proteins@net.bio.net Tue Mar 16 22:00:00 1993 Path: biosci!uwm.edu!spool.mu.edu!torn!nott!cunews!freenet.carleton.ca!Freenet.carleton.ca!ab885 From: ab885@Freenet.carleton.ca (G. Blake Steels) Newsgroups: bionet.molbio.proteins Subject: Myoglobin aggregation Message-ID: <1993Mar17.154532.7839@freenet.carleton.ca> Date: 17 Mar 93 15:45:32 GMT Sender: news@freenet.carleton.ca (News Administrator) Reply-To: ab885@Freenet.carleton.ca (G. Blake Steels) Organization: The National Capital Freenet Lines: 19 Does any one have a good data source for the aggregation of myoglobin as a function of pH? Thanks in advance for your help, Blake Steels steelsb@crl.aecl.ca ...or... ab885@freenet.carlton.ca -- _____________________________________________________________________________ G. Blake Steels ab885@Freenet.carleton.ca _____________________________________________________________________________ From owner-proteins@net.bio.net Mon Mar 22 22:00:00 1993 Path: biosci!uwm.edu!ux1.cso.uiuc.edu!news.cso.uiuc.edu!tsikar1.life.uiuc.edu!user From: David_Nunn@qms1.life.uiuc.edu (David Nunn) Newsgroups: bionet.molbio.proteins Subject: Broad host-range clone banks Message-ID: Date: 23 Mar 93 16:05:54 GMT Sender: usenet@news.cso.uiuc.edu (Net Noise owner) Followup-To: bionet.molbio.proteins Organization: University of Illinois (Microbiology) Lines: 15 We're looking for bacterial clone banks cloned into broad host range vectors. If you have a bank of any bacteria, including E. coli or Salmonella, in a broad host range vector, could you drop me a E-mail message. I can go into the details when you write, but we are looking for an enzyme with proteolytic and methylation activation that we think may be cryptic in many bacteria. Not to be picky but IncP vectors encoding tetracycline resistance would be ideal. Also, if you know someone who might have these types of banks and feel that they might be interested in sharing them, let me know. Thanks David Nunn, Ph.D. Department of Microbiology University of Illinois Urbana, IL 61801 (217) 333-6131 From owner-proteins@net.bio.net Tue Mar 23 22:00:00 1993 Path: biosci!s.u-tokyo!news.u-tokyo.ac.jp!wnoc-tyo-news!sh.wide!wnoc-kyo!icspub!oskgate0.mei!chorus.mei!adagio.panasonic.com!sgiblab!darwin.sura.net!haven.umd.edu!uunet!pipex!bnr.co.uk!uknet!daresbury!daresbury!news From: ccc_game@rzmain.rz.uni-ulm.de (Anton J. Gamel) Newsgroups: bionet.molbio.proteins Subject: 3rd ATTEMT: PLEASE UNSUBSCRIBE GAMEL@DULRUU51 Message-ID: <1993Mar24.191317.5258@gserv1.dl.ac.uk> Date: 24 Mar 93 18:40:43 GMT Sender: ccc_game@de.uni-ulm.rz.rzmain Distribution: bionet Lines: 477 Original-To: "biosci@daresbury.ac.uk"@1.decnet Original-Cc: "kristoff@net.bio.net"@1.decnet, "proteins@daresbury.ac.uk"@1.decnet Dear Listserver Administrator! This is the third MAIL of this kind I send to you! Let me first present a short HISTORY!: This history possibly gives an explanation about the many many requests for UNSUBSCRIBE sent to the mailing list. This is the reason why I sent this message to Dave Kristofferson and PROTEINS as a carbon copy. If you do not want to read the history, switch to EOF :-). --------------------------------------------------- > From: IE%"ADVISER@IRLEARN.UCD.IE" "Duty Adviser" 17-FEB-1993 18:46:48.49 > To: GAMEL@DULRUU51 > CC: > Subj: Relocation of +BIOJOBS distribution list > > Received: From IRLEARN(MAILER) by DULRUU51 with Jnet id 1481 > for GAMEL@DULRUU51; Wed, 17 Feb 1993 18:46 N > Received: from IRLEARN.UCD.IE (ADVISER) by IRLEARN.UCD.IE (Mailer R2.08) with > BSMTP id 6404; Wed, 17 Feb 93 17:47:46 GMT > Date: Wed, 17 Feb 93 17:47:38 GMT > From: Duty Adviser > Subject: Relocation of +BIOJOBS distribution list > To: GAMEL@DULRUU51 > > > Dear list subscriber, > > All Biosci mailing lists including +BIOJOBS which used to be located > at IRLEARN.UCD.IE have been relocated to Daresbury.AC.UK. If you were > subscribed to any of the Biosci lists at IRLEARN, your subscription > should have been automatically transferred to Daresbury. > > For further information and help with posting to any of the Biosci > lists or subscribing to and de-subscribing from the lists please send > mail to Biosci@Daresbury.AC.UK > > Commands similar to those accepted by LISTSERV may be sent to > list-server@Daresbury.ac.uk using mail. Send a HELP command for > further information. > > Those not located in Europe should subscribe to the distribution > point at Genbank. Further information about Genbank can be obtained > by sending mail to biosci@genbank.bio.net > > Thank you. > > Clare Gill > Duty Adviser, UCD Computing Services. > E-mail: ADVISER@IRLEARN.UCD.IE > Phone: 353-1-706-8645 > > What says "Pieces of seven, Pieces of seven"? > A Parroty error > ----------------------------------------------------------------------- > From: NETWAY::"POSTMASTER@dlgm.dl.ac.uk" 18-FEB-1993 13:52:12.62 > To: rzmain::ccc_game > CC: > Subj: Subscription to list. > > Address "ccc_game@rzmain.rz.uni-ulm.de" has been subscribed to > the "bionet-news.bionet.molbio.proteins" mail list by "ccc_game@rzmain.rz.uni-ulm.de" > > To mail the list send to "proteins@daresbury.ac.uk". > > The list administrators address is "biosci@daresbury.ac.uk" > > To remove yourself from this list send the command: > > UNSUB bionet-news.bionet.molbio.proteins > > to `List-Server@uk.ac.daresbury' > > Yours, > The daresbury List-Server > > ----------------------------------------------------------------------- > From: NETWAY::"POSTMASTER@dlgm.dl.ac.uk" 18-FEB-1993 13:52:37.01 > To: rzmain::ccc_game > CC: > Subj: Subscription to list. > > Address "ccc_game@rzmain.rz.uni-ulm.de" has been subscribed to > the "bionet-news.bionet.molbio.methds-reagnts" mail list by "ccc_game@rzmain.rz.uni-ulm.de" > > To mail the list send to "methods@daresbury.ac.uk". > > The list administrators address is "biosci@daresbury.ac.uk" > > To remove yourself from this list send the command: > > UNSUB bionet-news.bionet.molbio.methds-reagnts > > to `List-Server@uk.ac.daresbury' > > Yours, > The daresbury List-Server > ----------------------------------------------------------------------- > From: NETWAY::"POSTMASTER@dlgm.dl.ac.uk" 18-FEB-1993 13:53:09.83 > To: rzmain::ccc_game > CC: > Subj: Re: your requests > > Processing started: Thu Feb 18 12:47:37 1993 > > *return-path ccc_game@rzmain.rz.uni-ulm.de > > Address now "ccc_game@rzmain.rz.uni-ulm.de" > > *sub bionet-news.bionet.jobs > > Address `ccc_game@rzmain.rz.uni-ulm.de' has been subscribed to > the `bionet-news.bionet.jobs' list. The user has been informed. > > *sub bionet-news.bionet.molbio.methds-reagnts > > Address `ccc_game@rzmain.rz.uni-ulm.de' has been subscribed to > the `bionet-news.bionet.molbio.methds-reagnts' list. The user has been informed. > > *sub bionet-news.bionet.molbio.proteins > > Address `ccc_game@rzmain.rz.uni-ulm.de' has been subscribed to > the `bionet-news.bionet.molbio.proteins' list. The user has been informed. > > *grep gamel > > Searching for string "gamel" in all lists. > > WorkStation-Users - No matches found > > * > * Searching newsgroup bionet-news > * > - bionet.agroforestry - No matches found > - bionet.announce - No matches found > - bionet.general - No matches found > - bionet.software - No matches found > - bionet.journals.contents - gamel%patvcl.rz.uni-ulm.de@uk.ac.earn-relay > - bionet.jobs - GAMEL%DULRUU51@uk.ac.earn-relay > - bionet.molbio.methds-reagnts - GAMEL%DULRUU51@uk.ac.earn-relay > - bionet.molbio.proteins - GAMEL%DULRUU51@uk.ac.earn-relay > - bionet.sci-resources - No matches found > CPC-BULLETIN - No matches found > > [...] > > End of search. > > Processing end: Thu Feb 18 12:48:13 1993 > > ----------------------------------------------------------------------- > From: NETWAY::"POSTMASTER@dlgm.dl.ac.uk" 18-FEB-1993 13:59:00.11 > To: rzmain::ccc_game > CC: > Subj: Subscription to list. > > Address "ccc_game@rzmain.rz.uni-ulm.de" has been subscribed to > the "bionet-news.bionet.jobs" mail list by "ccc_game@rzmain.rz.uni-ulm.de" > > To mail the list send to "biojobs@daresbury.ac.uk". > > The list administrators address is "biosci@daresbury.ac.uk" > > To remove yourself from this list send the command: > > UNSUB bionet-news.bionet.jobs > > to `List-Server@uk.ac.daresbury' > > Yours, > The daresbury List-Server > > ----------------------------------------------------------------------- > From: NETWAY::"POSTMASTER@dlgm.dl.ac.uk" 18-FEB-1993 21:33:46.55 > To: "bionet.jobs mail newsgroup" > CC: > Subj: Re: Cancel subscription > > It looks like it is time for the standard reminder once again. > > Everyone, > > Please do not send mail about subscribing or canceling your > subscriptions to the newsgroup posting addresses. The appropriate > addresses to use are: > > > Address Location > ------- -------- > biosci@daresbury.ac.uk Europe, Africa, and Central Asia > biosci@net.bio.net Americas and the Pacific Rim > > > Sincerely, > > Dave Kristofferson > BIOSCI/bionet Manager > > kristoff@net.bio.net > > > ----------------------------------------------------------------------- > From: uk%"server-daemon@daresbury.ac.uk" 18-FEB-1993 23:13:01.43 > To: "bionet.jobs mail newsgroup" > CC: > Subj: Re: Cancel subscription > > Received: From UKACRL(MAILER) by DULRUU51 with Jnet id 1523 > for GAMEL@DULRUU51; Thu, 18 Feb 1993 23:13 N > Received: from RL.IB by UKACRL.BITNET (Mailer R2.07) with BSMTP id 6187; Thu, > 18 Feb 93 22:12:47 GMT > Received: from RL.IB by UK.AC.RL.IB (Mailer R2.07) with BSMTP id 4810; Thu, 18 > Feb 93 22:12:43 GMT > Via: UK.AC.DL.DLGM; 18 FEB 93 22:12:42 GMT > Received: by gserv1 (4.1/DL-V1.6) id AA15522 ( for dl_sun_server); Thu, 18 Feb > 93 19:39:51 GMT > Resent-From: > Date: 18 Feb 93 19:27:08 GMT > Resent-Date: Thu, 18 Feb 93 19:39:50 UT > To: "bionet.jobs mail newsgroup" > Message-Id: <93218193950.MIN-LZMAa25524.bionet-news@uk.ac.daresbury> > Resent-Message-Id: > From: kristoff@(David Kristofferson) net.bio.net > Reply-To: kristoff@(David Kristofferson) net.bio.net > Sender: "bionet.jobs mail newsgroup" > Subject: Re: Cancel subscription > X-Article-Number: bionet.jobs Msg # 597 > Comments: List problems/queries to > Comments: To mail both the group and netnews send to (biojobs@daresbury.ac.uk) > > It looks like it is time for the standard reminder once again. > > Everyone, > > Please do not send mail about subscribing or canceling your > subscriptions to the newsgroup posting addresses. The appropriate > addresses to use are: > > > Address Location > ------- -------- > biosci@daresbury.ac.uk Europe, Africa, and Central Asia > biosci@net.bio.net Americas and the Pacific Rim > > > Sincerely, > > Dave Kristofferson > BIOSCI/bionet Manager > > kristoff@net.bio.net > ----------------------------------------------------------------------- > From: NETWAY::"@dlgm.daresbury.ac.uk:List-Server@daresbury.ac.uk" 19-FEB-1993 03:30:17.73 > To: gamel > CC: > Subj: Re: your requests > > Processing started: Thu Feb 18 21:17:08 1993 > > *UNSUB bionet-news.bionet.molbio.proteins > > Address "gamel%de.uni-ulm.rz.rzmain@UK.AC.NSFNET-RELAY" was not found in the current > subscribers list for bionet-news.bionet.molbio.proteins > > NOTE! If you definitely are on this list then this failure > may have arisen from a different internal representation of > the address in question. You can either retry, specifying a > different address on the UNSUB command line, eg: > > UNSUB bionet-news.bionet.molbio.proteins J.Hacker@uk.ac.daresbury > > or you can contact the list administrator and ask him to > remove you from the list, his address is: > "biosci@daresbury.ac.uk" > > > *UNSUB bionet-news.bionet.molbio.methds-reagnts > > Address "gamel%de.uni-ulm.rz.rzmain@UK.AC.NSFNET-RELAY" was not found in the current > subscribers list for bionet-news.bionet.molbio.methds-reagnts > > NOTE! If you definitely are on this list then this failure > may have arisen from a different internal representation of > the address in question. You can either retry, specifying a > different address on the UNSUB command line, eg: > > UNSUB bionet-news.bionet.molbio.methds-reagnts J.Hacker@uk.ac.daresbury > > or you can contact the list administrator and ask him to > remove you from the list, his address is: > "biosci@daresbury.ac.uk" > > > *UNSUB bionet-news.bionet.jobs > > Address "gamel%de.uni-ulm.rz.rzmain@UK.AC.NSFNET-RELAY" was not found in the current > subscribers list for bionet-news.bionet.jobs > > NOTE! If you definitely are on this list then this failure > may have arisen from a different internal representation of > the address in question. You can either retry, specifying a > different address on the UNSUB command line, eg: > > UNSUB bionet-news.bionet.jobs J.Hacker@uk.ac.daresbury > > or you can contact the list administrator and ask him to > remove you from the list, his address is: > "biosci@daresbury.ac.uk" > > > Processing end: Thu Feb 18 21:17:11 1993 > > ----------------------------------------------------------------------- > From: NETWAY::"@dlgm.daresbury.ac.uk:List-Server@daresbury.ac.uk" 20-FEB-1993 14:30:48.22 > To: gamel > CC: > Subj: Re: your requests > > Processing started: Fri Feb 19 20:37:40 1993 > > *UNSUB bionet-news.bionet.molbio.proteins GAMEL%DULRUU51@uk.ac.earn-relay > > Sorry, you do not have permission to UNSUB users from the > `bionet-news' list. Please contact the list administrator > `biosci@daresbury.ac.uk' for unsubmissions from the list. > > *UNSUB bionet-news.bionet.molbio.methds-reagnts GAMEL%DULRUU51@uk.ac.earn-relay > > Sorry, you do not have permission to UNSUB users from the > `bionet-news' list. Please contact the list administrator > `biosci@daresbury.ac.uk' for unsubmissions from the list. > > *UNSUB bionet-news.bionet.jobs GAMEL%DULRUU51@uk.ac.earn-relay > > Sorry, you do not have permission to UNSUB users from the > `bionet-news' list. Please contact the list administrator > `biosci@daresbury.ac.uk' for unsubmissions from the list. > > Processing end: Fri Feb 19 20:37:46 1993 ------------------------------------------------ To complete, I may show my todays MAILDIRECTORY here: NEWMAIL # From Date Subject 1 uk%"server-daemon@da 22-MAR-1993 ! Resume: CDN, B.Sc. biotech, entry, lab and comp exp. 2 uk%"server-daemon@da 22-MAR-1993 histological sections of insects 3 NETWAY::"POSTMASTER@ 22-MAR-1993 histological sections of insects 4 NETWAY::"POSTMASTER@ 22-MAR-1993 Is Ph.D in USA or Europe possible? 5 uk%"server-daemon@da 22-MAR-1993 Is Ph.D in USA or Europe possible? 6 NETWAY::"POSTMASTER@ 22-MAR-1993 Re: oligo purification 7 uk%"server-daemon@da 22-MAR-1993 Re: oligo purification 8 NETWAY::"POSTMASTER@ 22-MAR-1993 TEST 9 uk%"server-daemon@da 22-MAR-1993 TEST 10 NETWAY::"POSTMASTER@ 22-MAR-1993 Re: PCR of a long genomic fragment... HELP? 11 uk%"server-daemon@da 22-MAR-1993 Re: PCR of a long genomic fragment... HELP? 12 NETWAY::"POSTMASTER@ 22-MAR-1993 Research Specialist 13 uk%"server-daemon@da 22-MAR-1993 Research Specialist 14 NETWAY::"POSTMASTER@ 22-MAR-1993 hi 15 uk%"server-daemon@da 22-MAR-1993 hi 16 NETWAY::"POSTMASTER@ 22-MAR-1993 Researchers in pollination or dispersal biology, Australia 17 uk%"server-daemon@da 22-MAR-1993 Researchers in pollination or dispersal biology, Australia 18 uk%"server-daemon@da 22-MAR-1993 M13 sequencing templates from XL1Blue 19 NETWAY::"POSTMASTER@ 22-MAR-1993 M13 sequencing templates from XL1Blue 20 uk%"server-daemon@da 22-MAR-1993 Re: Quick question on chemical name 21 NETWAY::"POSTMASTER@ 22-MAR-1993 Re: Quick question on chemical name 22 uk%"server-daemon@da 22-MAR-1993 Mouse Monoclonal Antibody Subtypes 23 NETWAY::"POSTMASTER@ 22-MAR-1993 Mouse Monoclonal Antibody Subtypes 24 NETWAY::"POSTMASTER@ 23-MAR-1993 Re: Software wanted for densitometry 25 uk%"server-daemon@da 23-MAR-1993 Re: Software wanted for densitometry 26 NETWAY::"POSTMASTER@ 23-MAR-1993 Re: M13 sequencing templates from XL1Blue 27 uk%"server-daemon@da 23-MAR-1993 Re: M13 sequencing templates from XL1Blue 28 NETWAY::"POSTMASTER@ 23-MAR-1993 RE: Mouse Monoclonal Antibody Subtypes 29 uk%"server-daemon@da 23-MAR-1993 RE: Mouse Monoclonal Antibody Subtypes 30 NETWAY::"POSTMASTER@ 23-MAR-1993 NovaTope Epitope Mapping 31 uk%"server-daemon@da 23-MAR-1993 NovaTope Epitope Mapping 32 NETWAY::"POSTMASTER@ 23-MAR-1993 Re: Death of a lambda library 33 NETWAY::"POSTMASTER@ 23-MAR-1993 POSTDOCTORAL RESEARCH FELLOWSHIP - CARDIAC TECHNOLOGY 34 NETWAY::"POSTMASTER@ 23-MAR-1993 Re: DidYouKnow... 35 uk%"server-daemon@da 23-MAR-1993 Re: Death of a lambda library 36 uk%"server-daemon@da 23-MAR-1993 POSTDOCTORAL RESEARCH FELLOWSHIP - CARDIAC TECHNOLOGY 37 uk%"server-daemon@da 23-MAR-1993 Re: DidYouKnow... 38 NETWAY::"POSTMASTER@ 23-MAR-1993 Re: precipitation of oligos 39 uk%"server-daemon@da 23-MAR-1993 Re: precipitation of oligos 40 NETWAY::"POSTMASTER@ 23-MAR-1993 Re: M13 sequencing templates from XL1Blue 41 uk%"server-daemon@da 23-MAR-1993 Re: M13 sequencing templates from XL1Blue 42 NETWAY::"POSTMASTER@ 23-MAR-1993 5' end-labelling with S-35-ATP 43 uk%"server-daemon@da 23-MAR-1993 5' end-labelling with S-35-ATP 44 NETWAY::"POSTMASTER@ 23-MAR-1993 Re: DidYouKnow... 45 uk%"server-daemon@da 23-MAR-1993 Re: DidYouKnow... 46 NETWAY::"POSTMASTER@ 23-MAR-1993 Re: Mouse Monoclonal Antibody Subtypes 47 uk%"server-daemon@da 23-MAR-1993 Re: Mouse Monoclonal Antibody Subtypes 48 NETWAY::"POSTMASTER@ 23-MAR-1993 resource management careers 49 NETWAY::"POSTMASTER@ 23-MAR-1993 Postdoctoral research associate position 50 NETWAY::"POSTMASTER@ 23-MAR-1993 Chemiluminescent Kits 51 uk%"server-daemon@da 24-MAR-1993 resource management careers 52 NETWAY::"POSTMASTER@ 24-MAR-1993 Information Resources Specialist (Albany, NY) 53 NETWAY::"POSTMASTER@ 24-MAR-1993 Re: Your choice of Reverse Transcriptase??? 54 NETWAY::"POSTMASTER@ 24-MAR-1993 Re: DidYouKnow... 55 NETWAY::"POSTMASTER@ 24-MAR-1993 How to check if DNA is transfered 56 NETWAY::"POSTMASTER@ 24-MAR-1993 (none) 57 NETWAY::"POSTMASTER@ 24-MAR-1993 "Looking for a Post Doctoral Position (Molecular Biology):July 1993" 58 NETWAY::"POSTMASTER@ 24-MAR-1993 Re: How to check if DNA is transfered 59 NETWAY::"POSTMASTER@ 24-MAR-1993 Perm Sys Admin; Taos Mountain Software; SF Bay Area 60 NETWAY::"POSTMASTER@ 24-MAR-1993 Re: DidYouKnow... 61 uk%"server-daemon@da 24-MAR-1993 Postdoctoral research associate position 62 NETWAY::"POSTMASTER@ 24-MAR-1993 Re: DidYouKnow... 63 NETWAY::"POSTMASTER@ 24-MAR-1993 Help- monoclonal antibodies against mouse antibody variable region 64 uk%"server-daemon@da 24-MAR-1993 Chemiluminescent Kits 65 uk%"server-daemon@da 24-MAR-1993 Information Resources Specialist (Albany, NY) 66 uk%"server-daemon@da 24-MAR-1993 Re: Your choice of Reverse Transcriptase??? 67 uk%"server-daemon@da 24-MAR-1993 Re: DidYouKnow... 68 uk%"server-daemon@da 24-MAR-1993 How to check if DNA is transfered 69 uk%"server-daemon@da 24-MAR-1993 Re: How to check if DNA is transfered 70 uk%"server-daemon@da 24-MAR-1993 Re: DidYouKnow... 71 uk%"server-daemon@da 24-MAR-1993 Help- monoclonal antibodies against mouse antibody variable region 72 uk%"server-daemon@da 24-MAR-1993 Perm Sys Admin; Taos Mountain Software; SF Bay Area 73 uk%"server-daemon@da 24-MAR-1993 Re: DidYouKnow... 74 uk%"server-daemon@da 24-MAR-1993 (none) 75 uk%"server-daemon@da 24-MAR-1993 "Looking for a Post Doctoral Position (Molecular Biology):July 1993" 76 uk%"server-daemon@da 24-MAR-1993 5' labelling with S-35 77 NETWAY::"POSTMASTER@ 24-MAR-1993 5' labelling with S-35 78 NETWAY::"POSTMASTER@ 24-MAR-1993 Re: Software wanted for densitometry 79 uk%"server-daemon@da 24-MAR-1993 Re: Software wanted for densitometry 80 NETWAY::"POSTMASTER@ 24-MAR-1993 Re: DidYouKnow... 81 uk%"server-daemon@da 24-MAR-1993 Re: DidYouKnow... 82 NETWAY::"POSTMASTER@ 24-MAR-1993 Re: DidYouKnow... 83 uk%"server-daemon@da 24-MAR-1993 Re: DidYouKnow... END OF HISTORY ---------------------------------------------------------------------------- My USER-ID has changed. After some LISTS have moved from IRLEARN to daresbury.ac.uk I tried to UNSUBSCRIBE, but the LISTSERV could not associate GAMEL%DULRUU51@uk.ac.earn-relay with any address I tried to formulate directly or via noumerous different GATEWAYS, bitnet or internet alike. Please remove me, * GAMEL%DULRUU51@uk.ac.earn-relay *, from the LISTS: - bionet.jobs - GAMEL%DULRUU51@uk.ac.earn-relay - bionet.molbio.methds-reagnts - GAMEL%DULRUU51@uk.ac.earn-relay - bionet.molbio.proteins - GAMEL%DULRUU51@uk.ac.earn-relay The entries of gamel%patvcl.rz.uni-ulm.de@uk.ac.earn-relay and ccc_game@rzmain.rz.uni-ulm.de are my subscriptions as well and should be kept. I may emphasize that I have NO FURTHER ACCESS to USER-ID GAMEL%DULRUU51. Only MAIL is forwarded. And as far as I know, subscriptions should be taken on the current valuable address. Many thanks With kind regards Anton J. Gamel If possible please reply directly to +-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-+ : : o o : : Anton J. Gamel : V : : Look, a Vet on VAX : (,,) : : : -""-- : +-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-+ From owner-proteins@net.bio.net Tue Mar 23 22:00:00 1993 Path: biosci!kristoff From: kristoff@net.bio.net (David Kristofferson) Newsgroups: bionet.molbio.proteins Subject: Re: 3rd ATTEMT: PLEASE UNSUBSCRIBE GAMEL@DULRUU51 Message-ID: Date: 25 Mar 93 01:39:50 GMT References: <1993Mar24.191317.5258@gserv1.dl.ac.uk> Distribution: bionet Organization: BIOSCI International Newsgroups for Biology Lines: 25 Anton, I have asked the BIOSCI folks at Daresbury to take care of your problem. Sorry for the inconvenience. On occasion there have been gateway problems in the U.K. If anyone has problems getting through, I have other ways to contact the Daresbury staff, so feel free to send mail to biosci@net.bio.net. The same situation holds true if there are problems reaching us; please contact biosci@daresbury.ac.uk. You should get action from at least one of these addresses fairly promptly, but I don't want to encourage the use of the newsgroups for resolving subscription problems, everyone. Sincerely, Dave Kristofferson BIOSCI/bionet Manager kristoff@net.bio.net P.S. - Everyone, please note that I read mail sent to my personal address, kristoff@net.bio.net, LAST. If I am on the road, as I will be next week to the FASEB meeting, there may be significant delays in my replying to messages sent to "kristoff." Please always use the biosci@net.bio.net address first for faster service. From owner-proteins@net.bio.net Mon Mar 29 23:00:00 1993 Path: biosci!bcm!cs.utexas.edu!natinst.com!news.dell.com!swrinde!zaphod.mps.ohio-state.edu!malgudi.oar.net!sun!oucsboss!oucsace!chargers!roy From: roy@chargers.ent.ohiou.edu (Partha Roy et240 Fall91) Newsgroups: bionet.molbio.proteins Subject: hGH(Human Growth Hormone) Message-ID: <1993Mar30.151120.10787@oucsace.cs.ohiou.edu> Date: 30 Mar 93 15:11:20 GMT Sender: usenet@oucsace.cs.ohiou.edu (Network News Poster) Organization: College Of Engg. & Tech., Ohio University. Athens Lines: 23 Originator: roy@chargers.ent.ohiou.edu I am a graduate student in Mech.Engg in Ohio University,Athens.I am doing research in Bio-molecular Engg. We are trying to predict the secondary structure of proteins through Artificial Intelligence (Neural Networks) techniques and molecular modelling of them in our Intergraph CAD workstation.We have successfully predicted the secon -dary structures of about 30 proteins and done molecular modelling of Bovine Growth Hormones(bGH),which was a joint venture of Edison Biotech Lab. and Mech.Engg. Department. Now we are trying to improve our prediction technique further by tr -aining the Fuzzy Art Map network.We also look forward to do molec- ular modelling of Human Growth Hormone(hGH) and change the configu- ration by breaking helix and replacing the residues. So,I badly need more informations about hGH. I was trying to get it from Protein Data Bank.But I could not find anything till now. I will be glad if anyone can send me the hgh.pdb file from Protein Data Bank which will be really helpful in our research. Looking forward to prompt responses. Thanks. -Partha Roy Email: roy@bobcat.ent.ohiou.edu Phone: (614)-594-5834 From owner-proteins@net.bio.net Tue Mar 30 23:00:00 1993 Path: biosci!uwm.edu!zaphod.mps.ohio-state.edu!wupost!uunet!pipex!marble.uknet.ac.uk!uknet!daresbury!daresbury!news From: M_MITCHELL@ICRF.ICNET.UK ("Smoke me a kipper. I'll be back for breakfast!") Newsgroups: bionet.molbio.proteins Subject: Periplasmic Preps Message-ID: <1993Mar31.120910.8307@gserv1.dl.ac.uk> Date: 31 Mar 93 12:08:00 GMT Sender: list-admin@daresbury.ac.uk Distribution: bionet Lines: 13 Original-To: PROTEINS@UK.AC.DARESBURY Path: wheel!m_mitchell From: m_mitchell@icrf.icnet.uk (Smoke me a kipper. I'll be back for breakfast!) Newsgroups: biosci.protein-analysis Subject: Periplasmic Preps Message-ID: <58608@icrf.icnet.uk> Date: 31 Mar 93 13:07:52 GMT Organization: Imperial Cancer Research Fund, London, UK Lines: 4 Does anyone out there in the Net know of a good method for purifying Ecoli periplasmic proteins? Michael Mitchell, Imperal Cancer Research Fund, London From owner-proteins@net.bio.net Wed Mar 31 23:00:00 1993 Path: biosci!uwm.edu!wupost!uunet!pipex!marble.uknet.ac.uk!mcsun!sunic!aun.uninett.no!nuug!nntp.uio.no!news From: OJOHNSEN@BIOMED.UIO.NO (yvind Johnsen) Newsgroups: bionet.molbio.proteins Subject: serine repeat Message-ID: <1993Apr1.120918.2723@ulrik.uio.no> Date: 1 Apr 93 12:09:18 GMT Sender: news@ulrik.uio.no (Mr News) Organization: NORWEGIAN EMBNET NODE Lines: 5 Nntp-Posting-Host: biomed.uio.no X-News-Reader: VMS NEWS 1.24 I am working on a new human protein that contains motifs found in nuclear proteins. It also contains a serine-rich region (85 % in 30 aa). I have searched protein databases with a serine repeat and I found several nuclear proteins with long serine repeats. Does someone know what function this repeat have?