From owner-proteins@net.bio.net Fri May 01 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!dca1-hub1.news.digex.net!digex!news.fas.harvard.edu!cherniav From: cherniav@fas.harvard.edu (Oksana Cherniavskaya) Newsgroups: bionet.molbio.proteins Subject: Cu(I) Plastocyanin Date: 2 May 1998 17:49:21 GMT Organization: Harvard University, Cambridge, Massachusetts Lines: 20 Message-ID: <6ifmb1$n5s$1@news.fas.harvard.edu> NNTP-Posting-Host: login3.fas.harvard.edu X-Newsreader: TIN [version 1.2 PL2] Hi, I am writing a paper trying to apply Marcus theory to electrong transfer in biological systems. Needless to say, the model I am planning to use is egregiously oversimplified. Nevertheless, I am looking for the data regarding the structure of the Cu(I) and Cu(II) Plastocyanin metal centers. Basically what I need are the bond lengths and Raman or IR stretching frequencies of the Cu(I)-S and Cu(I)-N bonds in these proteins. I found Raman and X-Ray studies of Cu(II) Plastocyanin in various random chemistry journals (after hours and hours in the library), but had absolutely no luck with Cu(I). I was wondering if such data is readily available, and if so where I could find it. I am very greatful for your advice. Thanks. -Oksana From owner-proteins@net.bio.net Fri May 01 23:00:00 1998 Path: biosci!agate!newsgate.duke.edu!galactose.mc.duke.edu.uucp!tschantz From: tschantz@galactose.mc.duke.edu (William P. Tschantz) Newsgroups: bionet.molbio.proteins Subject: Protein Expression Problems Date: 2 May 1998 13:04:38 GMT Organization: Duke University; Durham, N.C. Lines: 28 Distribution: world Message-ID: <6if5l6$lmr$1@news.duke.edu> NNTP-Posting-Host: galactose.mc.duke.edu HI I have a difficult protein expression problem to solve and thought someone out there may have some tips. I have recently cloned a novel gene from a human brain library. I know that i have the whole coding sequence since i can transcribe and translate it in vitro using a TNT system and it comigrates with the native purified protein (bovine). The original clone contained a large 3' UTR about 1.5 times larger than my coding sequence and a very short 5' region. I have made 7 different constructs for expression (2 differt CMV promoter vectors in COS cells, baculavirus, and E coli pET system) One set of constructs is the full length clone with 3' UTR and the other sets is where i have removed the 3' UTR. None of these constructs express at all based on western blot analysis with a decent antibody and by activity. Has anyone seen this before. I am planning to try other promoters in the mammalian system (SV40, adenovirus). Any hints would be greatly appreciated!! Thanks in advance. Bill -- | William R. Tschantz, Ph.D. * | | Dept of Pharmacology and Cancer Biology * | | Duke University, Durham, NC * Got Homebrew ?? | | tschantz@galactose.mc.duke.edu * | From owner-proteins@net.bio.net Fri May 01 23:00:00 1998 Path: biosci!agate!howland.erols.net!portc02.blue.aol.com!audrey02.news.aol.com!not-for-mail From: rick00100@aol.com (Rick00100) Newsgroups: bionet.molbio.proteins Subject: multimer on denatured SDS-PAGE? Lines: 15 Message-ID: <1998050302555700.WAA08931@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Date: 03 May 1998 02:55:57 GMT Organization: AOL http://www.aol.com Hi everyone, I'm a newbie in protein work and Western, and recently I'm trying to raise antibodies to my protein, which is about 22kd. I got some polyclonal Ab, and when I do a Western with CHO cells-transfected protein, I would not see a band with one of my Ab. However, after deglycosylating the cell lysates, I can see a band at around 40kd, and a faint band at 22kd. I'll try to do the appropriate peptide competition experiments when I get a hold of some peptides. At the mean time, can anybody tell my whether that 40kd band can be a dimer of my protein? I thought protein multimers (homo- or hetero-) would dissociate in a reducing condition (5% b-ME, 5% SDS + boiling 10 minutes). Thanks for the advice Ricky, JHMI From owner-proteins@net.bio.net Fri May 01 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!130.185.14.35!torn!newshost.uwo.ca!usenet From: Vadim Tsvetnitsky Newsgroups: bionet.molbio.proteins Subject: NP-40 and Triton X-100 Q! Date: Sat, 02 May 1998 19:30:13 -0400 Organization: London Regional Cancer Centre, ON, Canada Lines: 17 Message-ID: <354BAC85.778C@julian.uwo.ca> NNTP-Posting-Host: moses.lrcc.on.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win95; I) Hi, I always thought that Nonidet P40 and Triton X-100 are in fact different trademark names of the same detergent. Now I found couple of protocols for cell lysis which call for both NP40 and TX-100, each at 1% conc. Now I started wondering, may be I was wrong all the time? Could anyone shed some light on this? Thanks, -- Vadim Tsvetnitsky (vadtsvet@julian.uwo.ca) London Regional Cancer Centre 790 Commissioners Road East London, Ontario CANADA N6A 4L6 FAX 519-685-8616 Phone 519-685-8300 ext 3331 From owner-proteins@net.bio.net Sat May 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!128.230.129.106!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: rdudley@nrc.uab.edu Newsgroups: bionet.molbio.proteins Subject: Re: multimer on denatured SDS-PAGE? Date: Sun, 03 May 1998 01:57:44 -0600 Organization: Deja News - The Leader in Internet Discussion Lines: 34 Message-ID: <6ih4h8$liv$1@nnrp1.dejanews.com> References: <1998050302555700.WAA08931@ladder01.news.aol.com>#1/1 NNTP-Posting-Host: 130.160.7.41 X-Article-Creation-Date: Sun May 03 06:57:44 1998 GMT X-Http-User-Agent: Mozilla/4.03 [en] (Win95; I) In article <1998050302555700.WAA08931@ladder01.news.aol.com>#1/1, rick00100@aol.com (Rick00100) wrote: > > Hi everyone, > > I'm a newbie in protein work and Western, and recently I'm trying to raise > antibodies to my protein, which is about 22kd. I got some polyclonal Ab, and > when I do a Western with CHO cells-transfected protein, I would not see a band > with one of my Ab. However, after deglycosylating the cell lysates, I can see > a band at around 40kd, and a faint band at 22kd. I'll try to do the > appropriate peptide competition experiments when I get a hold of some peptides. > At the mean time, can anybody tell my whether that 40kd band can be a dimer of > my protein? I thought protein multimers (homo- or hetero-) would dissociate in > a reducing condition (5% b-ME, 5% SDS + boiling 10 minutes). > > Thanks for the advice > > Ricky, JHMI Actually, a number of receptors form noncovalent multimers that don't dissociate when boiled in BME or DTT and run on SDS-PAGE. Presents quite a problem for us. rich -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/ Now offering spam-free web-based newsreading From owner-proteins@net.bio.net Sat May 02 23:00:00 1998 From: Cornelius Krasel Subject: Re: multimer on denatured SDS-PAGE? Newsgroups: bionet.molbio.proteins References: <1998050302555700.WAA08931@ladder01.news.aol.com> User-Agent: tin/pre-1.4-980226 (UNIX) (Linux/2.0.32 (i486)) Date: Sun, 3 May 1998 18:41:42 +0200 Message-ID: <6o6ii6.sg.ln@wpxx02.toxi.uni-wuerzburg.de> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de Lines: 18 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.sprintlink.net!news.sprintlink.net!newsfeed.nacamar.de!uni-erlangen.de!uni-wuerzburg.de!not-for-mail Rick00100 wrote: > I'm a newbie in protein work and Western, and recently I'm trying to raise > antibodies to my protein, which is about 22kd. I got some polyclonal Ab, > and when I do a Western with CHO cells-transfected protein, I would not see > a band with one of my Ab. However, after deglycosylating the cell lysates, > I can see a band at around 40kd, and a faint band at 22kd. It's possible that the 40 kDa band is a dimer. Since your protein is a membrane protein (why else would you want to deglycosylate it), this is not entirely surprising. Sometimes it helps to omit the boiling step (at least this is true for some G protein-coupled receptors). --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP4 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-proteins@net.bio.net Sun May 03 23:00:00 1998 Message-ID: <354E2F11.BC150302@ikp.unibe.ch> Date: Mon, 04 May 1998 23:11:46 +0200 From: Zen X-Mailer: Mozilla 4.04 [en] (WinNT; I) MIME-Version: 1.0 Newsgroups: bionet.molbio.proteins Subject: Fe(III) Chelator Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit NNTP-Posting-Host: ikplab1a.unibe.ch Lines: 7 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!news-ge.switch.ch!news-zh.switch.ch!news.unibe.ch!ikplab1a.unibe.ch Does anyone know of a chelator for Fe(III)? Thank you! Regards, Zen From owner-proteins@net.bio.net Mon May 04 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!bofh.vszbr.cz!howland.erols.net!newshub.northeast.verio.net!nntp.upenn.edu!news.tju.edu!not-for-mail From: Charles Brenner Newsgroups: bionet.molbio.proteins Subject: Post-doc in Philly Date: Tue, 05 May 1998 15:24:30 -0400 Organization: Kimmel Cancer Institute, TJU Lines: 18 Message-ID: <354F676A.F39922C2@dada.jci.tju.edu> Reply-To: brenner@dada.jci.tju.edu NNTP-Posting-Host: cubist.jci.tju.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.05 (Macintosh; U; PPC) My laboratory has immediate funded openings for motivated post-docs who are interested in working in a multidisciplinary environment to unravel the function of a new superfamily of nucleotide-binding proteins. The proteins in question are Hint and Fhit and the methods employed range from X-ray crystallography to enzymology to yeast genetics. Interested candidates should send a letter of interest and include a publication list and the contact numbers for three referees. -- ********************************************************************** Charles Brenner Laboratory of Protein Structure Assistant Professor and Cellular Function Kimmel Cancer Institute phone (215) 503-4573 Thomas Jefferson University fax (215) 923-2117 233 S 10th St., rm. 826 mailto:brenner@dada.jci.tju.edu Philadelphia, PA 19107 http://asterix.jci.tju.edu/brenner.html ********************************************************************** From owner-proteins@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!genetics.com!mstahl From: mstahl@genetics.com (Mark Stahl) Newsgroups: bionet.molbio.proteins Subject: Staff Scientist-Genetics Institute, Cambridge MA Date: 6 May 1998 04:25:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net STAFF SCIENTIST-Genetics Institute, Cambridge, MA A staff scientist is sought to join a group dedicated to purifying and characterizing recombinant proteins for structural determination by X-ray crystallography and multi-dimensional NMR. This group is part of a team which is developing new drugs through structure-based design. Candidates must have a Ph. D. in biochemistry or molecular biology with 2-6 years of postdoctoral experience. Preference will be given to candidates who have experience in homology modeling and are interested in protein structure/function. Good communication and team skills in a multidisciplinary environment of molecular biologists, biochemists, biophysicists, and chemists are required. Please respond by sending your resume to mstahl@genetics.com Mark Stahl, Ph.D. Senior Scientist SMDD Genetics Institute, Inc. 85 Bolton St. Cambridge, MA 02140 From owner-proteins@net.bio.net Tue May 05 23:00:00 1998 From: "Echo" Newsgroups: bionet.molbio.proteins Subject: Creative ?? Want to do more?? Date: Sun, 26 Apr 1998 17:17:29 -0500 Lines: 7 X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 NNTP-Posting-Host: 199.174.253.72 Message-ID: <3543b24a.0@news.compuzone.net> Path: biosci!news.Stanford.EDU!Cabal.CESspool!news-feed.inet.tele.dk!bofh.vszbr.cz!novia!sws1.ctd.ornl.gov!pstcc7.pstcc.cc.tn.us!198.146.194.20!news.compuzone.net!199.174.253.72 check http://www.a-ten.com/books/ub/ for unique books! Brain candy for the creative and original. From owner-proteins@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!news-peer-west.sprintlink.net!news-peer.sprintlink.net!news-backup-east.sprintlink.net!news.sprintlink.net!207.217.77.43!newsfeed1.earthlink.net!nntp.earthlink.net!usenet From: John Philo <"jphilo*NO SPAM12*"@earthlink.net> Newsgroups: bionet.molbio.proteins Subject: Re: centricon columns Date: Wed, 06 May 1998 08:57:55 -0700 Organization: Alliance Protein Laboratories Lines: 33 Message-ID: <35508883.4C04@earthlink.net> References: <354608A1.1175@york.ac.uk> Reply-To: jphilo*NO, SPAM12*@earthlink.net NNTP-Posting-Host: 207.217.142.53 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.03 (Win95; I) To: djs17@york.ac.uk djs17@york.ac.uk wrote: > > Hia, > Has anyone had problems with centricon-10 columns aggregating proteins? > We had a sample that sedimented at 3s before concentration, and 20 s > afterwards. It is known to be a tetramer of around 44 kDa, so the larger > sedimentation coefficient is due to aggregation. > > your views please.... > > Dave. Centricons can definitely cause aggregation and precipitation, through at least two different mechanisms. One mechanism is simply that the concentration right at the membrane surface can get extremely high (much higher than the bulk concentration). You can reduce this effect somewhat by slowing down the rate of concentration, allowing more time for diffusion. The second effect is due to surface binding and adsorption of your protein, which leads to conformational changes that promote aggregation. The culprit here is often the ultrafiltration membrane (and again the high local concentration promotes the binding), but it can also be the container walls. People have had some success in reducing this problem by pre-treating the Centricon's with glycerol. This presumably covers up some of the 'sticky' sites, but the drawback is that even after you pour out the glycerol some will remain and some will be present in your concentrated sample. John Philo, Alliance Protein Laboratories *** Remove "*NO SPAM12*" from return address before replying. *** From owner-proteins@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!europa.clark.net!192.174.65.44!newscore.univie.ac.at!193.171.255.24.MISMATCH!newsfeed03.univie.ac.at!03-newsfeed.univie.ac.at!news.univie.ac.at!not-for-mail From: a8803349.nospam@unet.univie.ac.at (Martin Offterdinger) Newsgroups: bionet.molbio.proteins Subject: Re: NP-40 and Triton X-100 Q! Date: Wed, 06 May 1998 07:25:49 GMT Organization: AKH Lines: 30 Message-ID: <35501027.5972160@news.univie.ac.at> References: <354BAC85.778C@julian.uwo.ca> NNTP-Posting-Host: grunt.imed1.akh-wien.ac.at X-Newsreader: Forte Free Agent 1.1/16.230 On Sat, 02 May 1998 19:30:13 -0400, Vadim Tsvetnitsky wrote: >Hi, >I always thought that Nonidet P40 and Triton X-100 are in fact different >trademark names of the same detergent. Now I found couple of protocols >for cell lysis which call for both NP40 and TX-100, each at 1% conc. >Now I started wondering, may be I was wrong all the time? Could anyone >shed some light on this? > >Thanks, > >-- >Vadim Tsvetnitsky (vadtsvet@julian.uwo.ca) >London Regional Cancer Centre >790 Commissioners Road East >London, Ontario >CANADA N6A 4L6 >FAX 519-685-8616 >Phone 519-685-8300 ext 3331 Triton X-100 and NP40 are in fact similar from their chemical structure but still there is a difference; NP40 has a methyl group that is missing in tritonx100. Martin Offterdinger Internal Med.I,Dept. Oncology University of Vienna Austria E-Mail:a8803349.nospam@unet.univie.ac.at (remove nospam before mailing) From owner-proteins@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!208.134.241.18!newsfeed.internetmci.com!205.252.116.205!howland.erols.net!Cabal.CESspool!bofh.vszbr.cz!serra.unipi.it!newsserver.cilea.it!news.csi.unimi.it!not-for-mail From: paolo.castano@unimi.it (Prof. Paolo Castano) Newsgroups: bionet.molbio.proteins Subject: Photomicrography Course Date: Wed, 06 May 1998 13:20:22 GMT Organization: Universita' degli Studi di Milano Lines: 31 Message-ID: <35506395.23997258@news.unimi.it> NNTP-Posting-Host: isdn30.csi.unimi.it X-Newsreader: Forte Free Agent 1.1/32.230 INTERNATIONAL COURSES OF LIGHT MICROSCOPY, PHOTOMICROGRAPHY AND LASER SCANNING CONFOCAL MICROSCOPY GARGNANO (Lake of Garda) October 1998 The Course is a post-graduated theoretical/practical course, with propedeutical lectures and practical stages on microscopy, photomicrography and confocal microscopy. The course will take place in Gargnano (Lake of Garda) in October 1998. Further information and registration details will be found at the following Web address. http://imiucca.csi.unimi.it/endomi/micro.html Thank you Paolo Castano _______________________________________________ Prof. Paolo Castano UNIVERSITY of MILAN Institute of Human Anatomy CHAIR OF HUMAN ANATOMY - FACULTY OF PHARMACY Via Mangiagalli 31 - 20133 Milan (Italy) - Tel. 39.2.26.63.683 Fax 39.2.23.64.082 / 39.2.70.63.54.25 e-mail: clsmteam@imiucca.csi.unimi.it From owner-proteins@net.bio.net Tue May 05 23:00:00 1998 Path: biosci!BIOSCI.CBS.UMN.EDU!gardner From: gardner@BIOSCI.CBS.UMN.EDU (Nancy Gardner) Newsgroups: bionet.molbio.proteins Subject: mass spec Date: 6 May 1998 08:34:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I would appreciate it if someone could notify me of a mass spec lab that can do MALDI-MS or electrospray.I have a mixture of peptides, one for which I would like to get verification of a partial sequence and modification. Thanks in advance, Nancy Gardner gardner@biosci.cbs.umn.edu From owner-proteins@net.bio.net Wed May 06 23:00:00 1998 From: "Performance Technology" Subject: Technical Service Manager Date: Thu, 7 May 1998 10:42:03 -0700 Lines: 23 X-Newsreader: Microsoft Outlook Express 4.72.2106.4 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4 Message-ID: Newsgroups: bionet.molbio.proteins Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!207.68.152.14!upnetnews04!upnetnews03 We are working with a company who is considered the "cadillac" of the custom immunology business located in Northern California. We are looking for a Service Manager who would interface extensively with leading investigators,and industry clients. This individual would also manage assistants who would coordinate customer requirements with in-house production. This position is highly visible and call for an indvidual who has not only excellent technical skills but superior communication skills. This would be a good position for a person with a Bachelors degree in Life Sciences who is currently working at the bench or in customer service and who wants to broaden their experience and interface more extensively with investigators. The compensation is between $40-000 to $45,000 with excellent benefits. Please contact Dish Taylor at Ty_n_Dish@msn.com Phone number 310-394-7858 From owner-proteins@net.bio.net Wed May 06 23:00:00 1998 From: stebby@welchlink.welch.jhu.edu (Stephen C. Dahl) Newsgroups: bionet.molbio.proteins Subject: Re: multimer on denatured SDS-PAGE? Date: 5 May 1998 18:53:35 GMT Organization: HCF - Johns Hopkins University, Baltimore, Maryland, USA Lines: 20 Message-ID: <6inn7f$2ku@news.jhu.edu> References: <1998050302555700.WAA08931@ladder01.news.aol.com> <6o6ii6.sg.ln@wpxx02.toxi.uni-wuerzburg.de> NNTP-Posting-Host: 128.220.59.78 X-Newsreader: TIN [version 1.2 PL2] Path: biosci!agate!newsfeed.wli.net!news-xfer.netaxs.com!news4.his.com!news.cs.jhu.edu!news.jhu.edu!welchlink!stebby Cornelius Krasel (krasel@wpxx02.toxi.uni-wuerzburg.de) wrote: : Rick00100 wrote: : > I'm a newbie in protein work and Western, and recently I'm trying to raise : > antibodies to my protein, which is about 22kd. I got some polyclonal Ab, : > and when I do a Western with CHO cells-transfected protein, I would not see : > a band with one of my Ab. However, after deglycosylating the cell lysates, : > I can see a band at around 40kd, and a faint band at 22kd. : It's possible that the 40 kDa band is a dimer. Since your protein is : a membrane protein (why else would you want to deglycosylate it), this : is not entirely surprising. Sometimes it helps to omit the boiling step : (at least this is true for some G protein-coupled receptors). Agreed. We work on proteins with multiple membrane spanning domains. One of them becomes a mass of goo that will not enter the gel if its boiled. Heating at temperatures no greater than 65' for 3--5 minutes does the trick. Hey, and Rick, if you're here at JHMI how come you're not on welchlink? From owner-proteins@net.bio.net Thu May 07 23:00:00 1998 Path: biosci!acdlabs.com!vic From: vic@acdlabs.com ("Victor Graziano") Newsgroups: bionet.molbio.proteins Subject: Free Protein Manager Software Demo Date: 8 May 1998 07:20:27 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Distribution: world Message-ID: <000701bd7aa5$6a214640$3ee9b0cf@vic.acdlabs.com> NNTP-Posting-Host: net.bio.net Dear Colleagues Advanced Chemistry Development Inc. (ACD), a leader in Cheminformatics software, has just released Protein Manager, the newest member of its extensive software family. This Bioinformatics software package, coupled with the Swissprot, Prosite, PIR, PDB, ACD Restriction Enzyme and (soon to follow) ACD Regulatory Protein Databases, will dramatically accelerate protein research and peptide drug discovery. ACD/Protein Manager is built around a three-in-one functionality concept which provides all the necessary features to Analyze, Publish (Web & Desktop) and Manage protein sequence data. Software operation is easy, simply load candidate proteins into the Protein Manager 'protein workbook' and perform a multitude of functions including: -Isoelectric point determination -Batch Phys-Chem property predictions -Protein secondary structure prediction (including the newest Predator prediction method) -Multiple protein alignments with consensus matching -Enzymatic digestion of selected proteins (utilizing ACD's enzyme database) -Protein scale determination (hydrophobicity, polarity, bulkiness...) -3D viewing of all available Protein Database (PDB) files More information outlining Protein Manager's functionality, along with a software demo, short product video and future software releases (ACD Regulatory peptide DB or ACD/Gene Manager) are available at: http://www.acdlabs.com/products/peptide/prot_mgr.html. Please feel free to contact myself or any of the other ACD representatives for further information regarding Protein Manager or any of the other ACD software titles. Regards, Victor Graziano B.Sc., M.Sc. Advanced Chemistry Development, Inc. Account Manager (Biochemistry) T: (416) 368-3435 F: (416) 368-5596 US & Canada: (800) 304-3988 http://www.acdlabs.com From owner-proteins@net.bio.net Thu May 07 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.concentric.net!news-peer-west.sprintlink.net!news.sprintlink.net!newsfeed.direct.ca!news-peer.gip.net!news.gsl.net!gip.net!newsfeed.nacamar.de!nntp.news.xara.net!xara.net!server5.netnews.ja.net!daresbury!not-for-mail From: "<<"<>@localhost Newsgroups: bionet.molbio.proteins Subject: Ad: Your Personal Invitation To Financial Freedom Date: 8 May 1998 17:49:26 +0100 Lines: 48 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <6ivd2m$d2b@mserv1.dl.ac.uk> Original-To: Your Personal Invitation To Financial Freedom Have you ever felt dissatisfied or unfulfilled at your current job? Are you tired of working for someone else? Have you ever longed to make a difference on the planet? Would you like to learn how to harness your full potential so that you can get a whole lot more out of life? You can generate a substantial income marketing our educational products - courses and seminars, in the Personal Growth and Self Empowerment field. This is a $38 billion dollar industry that is growing is by double digits annually. These numbers speak volumes about people's fervent desire to improve the quality and conditions of their lives, to experience a life filled with prosperity, excellent relationships, and personal satisfaction - a life with meaning and purpose - where we can make a difference for others, and a real contribution to the world. We can offer you: A true work at home business - using your telephone, fax and/or the internet. A 90% gross profit margin - Not MLM! No inventory No personal selling, automated calls do all the selling and telling for you Complete training and support, personal coaching International Marketplace For more information: Call 1-800-461-0773 When the recording answers, press 1 as a first time caller, the extension is 181. After the brief message, please leave your name, telephone # (include area code) and a good time to reach you. (Start up capital required) ************************************************************************ Help us keep our lists clean. To be removed, email mailto:listmgmt@iname.com with "Remove" in the SUBJECT line. Thank you. From owner-proteins@net.bio.net Fri May 08 23:00:00 1998 Path: biosci!DAWGNATION.COM!dawgmaster From: dawgmaster@DAWGNATION.COM Newsgroups: bionet.molbio.proteins Subject: DawgVent Account for HspDawg Date: 9 May 1998 12:29:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <199805091926.PAA08505@www12.clever.net> NNTP-Posting-Host: net.bio.net This is an automated response. Your DawgVent account is now active. Username: HspDawg Password: ugav You may change your password by visiting the registration page and submitting a password change. It is not necessary to respond to this message. The Junkyard From owner-proteins@net.bio.net Sat May 09 23:00:00 1998 Newsgroups: bionet.molbio.proteins Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!logbridge.uoregon.edu!europa.clark.net!192.148.253.68!netnews.com!ix.netcom.com!atlantis From: atlantis@netcom.com (JJ Miranda) Subject: favorite biochem links? Message-ID: Organization: Netcom On-Line Services X-Newsreader: TIN [version 1.2 PL2] Date: Sun, 10 May 1998 19:22:31 GMT Lines: 7 Sender: atlantis@netcom9.netcom.com I'm trying to compile a collection for commonly used links for biochemistry (especially protein chemistry). If anyone could recommend sites or links that they use frequently, please email the address to me. I'm planning on placing all the links in an organized web site for reference. Sincere regards, JJ Miranda From owner-proteins@net.bio.net Sat May 09 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!logbridge.uoregon.edu!news-peer.gip.net!news.gsl.net!gip.net!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: pyoung@eng.utoledo.edu Newsgroups: bionet.molbio.proteins Subject: Information Request: Differential Scanning Calorimeter Date: Sun, 10 May 1998 08:41:31 GMT Organization: Deja News - The Leader in Internet Discussion Lines: 32 Message-ID: <6j3p7r$vjm$1@nnrp1.dejanews.com> NNTP-Posting-Host: 131.183.16.71 X-Article-Creation-Date: Sun May 10 08:41:31 1998 GMT X-Http-User-Agent: Mozilla/4.0 (compatible; MSIE 4.01; Windows NT) Hello, Does anybody ever use Differential Scanning Calorimeter produced by Calorimetry Science Corporation located in Provoo, Utah? In particular, I would like to know whether or not anyone has purchased their CSC 4100 model multi-cell differential scanning calorimeter. I am having quite a bit of problems with this scanning calorimeter. It has a habit of burning up and emitting smell of burning plastics. I am wondering whether or not this instrument is designed corretctly. I had two of these instruments in the last one and half years and they never operated beyond more than two months. The one I just received happend to burn up less than 48 hrs after I received it. By the way, does anybody know which company manufacture multi-celled DSC capable of temperature range between -20°C and 200°C. It seems like less than a handful of companies produced these instruments. Thank you for all your help. Sincrely, Paul -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/ Now offering spam-free web-based newsreading From owner-proteins@net.bio.net Sat May 09 23:00:00 1998 Path: biosci!rutgers!rockyd.rockefeller.edu!newsfeed.nyu.edu!news.idt.net!news-peer-east.sprintlink.net!news-peer.sprintlink.net!news.sprintlink.net!howland.erols.net!newsfeed.internetmci.com!207.217.77.43!newsfeed1.earthlink.net!nntp.earthlink.net!usenet From: The Antibody Resource Page Newsgroups: bionet.molbio.proteins Subject: Looking for an antibody? See the Antibody Resource Page (www.antibodyresource.com)!!! Date: Sun, 10 May 1998 18:18:27 +0000 Organization: EarthLink Network, Inc. Lines: 46 Message-ID: <3555EF74.14E3@antibodyresource.com> NNTP-Posting-Host: 208.255.9.147 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.04Gold (Macintosh; I; 68K) The Antibody Resource Page (http://www.antibodyresource.com/) is an invaluable website to researchers and educators. Here is just some of what can be found on the page: 1. How to Find an Antibody - a variety of ways on and off the web to find the antibody you are looking for. There are links to free search engines that allow you to search a multitude of companies for the specific antibody that you need. 2. Online Companies - links to over 110 companies that sell antibodies or antibody related products. Is your company listed on this page? 3. Antibody Image Gallery - see some of the latest in animated and non-animated antibody graphics 4. Bulletin Board - Have a question? Then stop by and post a message. 5. Educational Resources - a variety of new links have been added.There are links to pages on immunochemistry, antibody production, autoimmunity, vaccines, immunology and much more. This page is divided up into sections on research, educational, and health resources. 6. The latest in antibody news - Get up-to-date, antibody-related news articles and newsgroup discussions 7. Online databanks and databases - links to online protein and nucleic acid sequencing databases. Invaluable to biochemists and molecular biologists who work with antibodies. Check it all out at: http://www.antibodyresource.com/ ps. Don't forget to visit our current sponsors, Diatec (http://www.diatec.com/) and Research Diagnostics, Inc.(http://www.researchd.com/absort1.htm). pps. Are you interested in being a sponsor on the Antibody Resource Page (ARP)? Nearly half of the visitors come to find commercially available antibodies. If you are a company that sells antibodies and want to increase webtraffic to your site, email the antibody resource page to find out about sponsorship: antibody@antibodyresource.com ppps. The ARP was voted among the top 25 biotechnology webpages for 1997 by Genetic Engineering News! From owner-proteins@net.bio.net Sun May 10 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ix.netcom.com!news From: Mark Atlas Newsgroups: bionet.molbio.proteins Subject: New Services on Going Going Sold! Online auction for used lab equipment Date: Mon, 11 May 1998 15:00:27 GMT Organization: Netcom Lines: 117 Message-ID: <6j73v0$clq@dfw-ixnews6.ix.netcom.com> NNTP-Posting-Host: sjx-ca115-56.ix.netcom.com X-NETCOM-Date: Mon May 11 10:02:56 AM CDT 1998 X-Newsreader: NETCOMplete/4.0 New Services on Going Going Sold! Online auction for used lab equipment Remember that piece of equipment that you only wished you had but could not afford. You also did not want to spend the time to find this item as it could take you days or weeks to succeed or even fail since your network of sellers was limited. Going, Going...Sold! ( http://www.going-going-sold.com ) has just added two exciting new features to it's successful auction format for used lab equipment Custom Equipment Search Services: This new service has come off with a bang. Just contact us ( service@going-going-sold.com ) and let us know what item you want that we are not carrying on our auction site. Our technical staff will contact you and qualify your needs. Remember our staff are not brokers, but rather experienced and technically savvy ex sales reps from large analytical companies who can precisely qualify your needs. After needs are assessed, we will pass the information to our broker alliances who will begin the searches in hundreds of companies. If it is out there , it will be found. No longer will you miss out on an opportunity to buy a needed piece of equipment for a great price without having to lift a finger, and go through time consuming and tedious negotiations. Custom Equipment leases of used wares: Now that we have located that hard to find item, the budget is still insufficient. Terrie Hawley at Preferred financial has established a custom program for our clients to allow them to easily qualify for a lease to own program. Imagine that $10,000 GC @ $300.00/ month or a fume hood, , laminar flow hood and microscope combination at $200.00 month. Sounds too good to be true. come visit us @ http://www.going-going-sold.com or email us @ service@going-going sold.com Below is a a partial list of items upcoming on the block ***************************************************** Sale Closing : 5/15/98 Opening Prices ***************************************************** 1. Sorvall RC 3B and Rotor $7,200.00 ------------------------------------------------------------ 2. Centra 4B $1,395.00 ------------------------------------------------------------ 3. Direct Blotting DNA Sequencer $2,000.00 ------------------------------------------------------------ 4. Tangential Flow System $15,000.00 ------------------------------------------------------------ 5. Beckman L5-75 Centrifuge $6,500.00 ------------------------------------------------------------ 6. Beta Scint. Counter $13,000.00 ------------------------------------------------------------ 7. Beckman DU-70 $4,750.00 ------------------------------------------------------------ 8. Model 996 PDA Detector $4,750.00 ------------------------------------------------------------ 9. Capillary Rheometer System $4,750.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/20/98 Opening Prices ***************************************************** 1. Beckman GPR Centrifuge $2,900.00 ------------------------------------------------------------ 2. Beckman TJ-6R Centrifuge $2,100.00 ------------------------------------------------------------ 3. Beckman DU-62 $4,000.00 ------------------------------------------------------------ 4. ATD-400 with MCS-16 $22,000.00 ------------------------------------------------------------ 5. HP1090 Diode Array w/ autosampler $12,000.00 ------------------------------------------------------------ 6. Bio-safety hood 4 foot with stand Type IIA, or IIB $2,200.00 ------------------------------------------------------------ 7. Optima Ultracentrifuge $20,000.00 ------------------------------------------------------------ 8. Oligo 1000 $1,500.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/22/98 Opening Prices ***************************************************** 1. Beckman L5-50 Centrifuge $3,400.00 ------------------------------------------------------------ 2. Beckman L7-65 Ultra Centrifuge $4,850.00 ------------------------------------------------------------ 3. Purifier Class II $2,300.00 ------------------------------------------------------------ 4. Amino Acid Analyzer $35,000.00 ------------------------------------------------------------ 5. Tekmar 2000 sampler $6,900.00 ------------------------------------------------------------ 6. Liquid Chromatograph $27,750.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/27/98 Opening Prices ***************************************************** 1. Beckman DU-7 $3,500.00 ------------------------------------------------------------ 2. HP MS Engine/Electrospray System $63,000.00 ------------------------------------------------------------ 3. HP 5890 GC $12,000.00 ------------------------------------------------------------ 4. Varian 340 GC $8,500.00 ------------------------------------------------------------ 5. Waters HPLC System (newer)600E/486/717 $15,300.00 ------------------------------------------------------------ ***************************************************** (c) 1997, Internet Auctioneers International From owner-proteins@net.bio.net Sun May 10 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.ecrc.net!newsfeed.nacamar.de!univ-lyon1.fr!cri.ens-lyon.fr!NewsWatcher!user From: fmallet@ens-bma.cnrs.fr (François Mallet) Newsgroups: bionet.molbio.proteins Subject: albumin Date: Mon, 11 May 1998 16:49:02 +0100 Organization: UMR 103 CNRS/Biomerieux Lines: 11 Message-ID: NNTP-Posting-Host: fox-trot.ens-bma.cnrs.fr We try to purify a protein from human urine. The goal of our our work is to sequence this protein that have an biological activity. However, after multiple steps of purification, and although the specific activity of the enriched protein increased, this protein remains contaminated with large amounts of albumin. Thus we are unable to sequence it. Could you give me some advices on the way to discard the albumin. Thank you Francois mallet From owner-proteins@net.bio.net Sun May 10 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!logbridge.uoregon.edu!Cabal.CESspool!bofh.vszbr.cz!newsfeed.eris.dera.gov.uk!delos!server1.netnews.ja.net!warwick!leicester!usenet From: "Dr E. Buxbaum" Newsgroups: bionet.molbio.proteins Subject: Re: albumin Date: Mon, 11 May 1998 16:55:32 -0700 Organization: University of Leicester (PCFS User) Lines: 18 Message-ID: <35578FF4.5086@le.ac.uk> References: NNTP-Posting-Host: pc47.msb.le.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.01 (Win16; I) François Mallet wrote: > > We try to purify a protein from human urine. The goal of our our work is > to sequence this protein that have an biological activity. However, after > multiple steps of purification, and although the specific activity of the > enriched protein increased, this protein remains contaminated with large > amounts of albumin. Thus we are unable to sequence it. How do you tell that there is albumine contamination? The method which gives you that information can also be used to purify the albumine away. Remeber that for sequencing purpose you need inly a few hundred picomole of your protein, that much can be isolated for example by SDS-PAGE and blotting onto PVDF membranes. Stain with Coomassie the usual way, cut out the bands and give the snippets to the sequencing laboratory. The PVDF serves as a carrier during the sequencing process. If you need more help, than you have to give us more info on your protein. From owner-proteins@net.bio.net Mon May 11 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!feed1.news.luth.se!luth.se!news-stkh.gip.net!news.gsl.net!gip.net!newsfeed1.funet.fi!news.kolumbus.fi!news.uninet.ee!kadri.ut.ee!not-for-mail From: Peeter Toomik Newsgroups: bionet.molbio.proteins Subject: Re: albumin Date: Tue, 12 May 1998 12:08:00 +0300 Organization: Tartu University Lines: 21 Message-ID: <35581170.384A6EC3@ut.ee> References: NNTP-Posting-Host: biochem3.bio.ut.ee Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Trace: kadri.ut.ee 894963879 21431 (None) 193.40.8.203 X-Complaints-To: usenet@kadri.ut.ee X-Mailer: Mozilla 4.05 [en] (Win95; I) François Mallet wrote: > We try to purify a protein from human urine. The goal of our our work is > to sequence this protein that have an biological activity. However, after > multiple steps of purification, and although the specific activity of the > enriched protein increased, this protein remains contaminated with large > amounts of albumin. Thus we are unable to sequence it. > > Could you give me some advices on the way to discard the albumin. > > Thank you > > Francois mallet I would first try affinity chromatography on immobilised Cibacron Blue dye (e.g. Blue Sepharose), it binds albumin very strongly. Peeter Toomik From owner-proteins@net.bio.net Tue May 12 23:00:00 1998 Path: biosci!internet!biosci!not-for-mail From: daemon@net.bio.net Newsgroups: bionet.molbio.proteins Subject: Ad: Your Personal Invitation To Financial Freedom Date: 13 May 1998 15:01:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Distribution: world Message-ID: <199805132201.PAA29127@net.bio.net> NNTP-Posting-Host: net.bio.net Your Personal Invitation To Financial Freedom Have you ever felt dissatisfied or unfulfilled at your current job? Are you tired of working for someone else? Have you ever longed to make a difference on the planet? Would you like to learn how to harness your full potential so that you can get a whole lot more out of life? You can generate a substantial income marketing our educational products - courses and seminars, in the Personal Growth and Self Empowerment field. This is a $38 billion dollar industry that is growing is by double digits annually. These numbers speak volumes about people's fervent desire to improve the quality and conditions of their lives, to experience a life filled with prosperity, excellent relationships, and personal satisfaction - a life with meaning and purpose - where we can make a difference for others, and a real contribution to the world. We can offer you: A true work at home business - using your telephone, fax and/or the internet. A 90% gross profit margin - Not MLM! No inventory No personal selling, automated calls do all the selling and telling for you Complete training and support, personal coaching International Marketplace For more information: Call 1-800-461-0773 When the recording answers, press 1 as a first time caller, the extension is 181. After the brief message, please leave your name, telephone # (include area code) and a good time to reach you. (Start up capital required) ************************************************************************ Help us keep our lists clean. To be removed, email mailto:listmgmt@iname.com with "Remove" in the SUBJECT line. Thank you. From owner-proteins@net.bio.net Tue May 12 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!bofh.vszbr.cz!newscore.univie.ac.at!news-ge.switch.ch!news-zh.switch.ch!elna.ethz.ch!not-for-mail From: "Stephane Vuilleumier" Newsgroups: bionet.molbio.proteins Subject: pH optimum of enzymes and physiological pH Date: Wed, 13 May 1998 09:28:34 +0200 Organization: Swiss Federal Institute of Technology (ETHZ) Lines: 24 Message-ID: <6jbhsi$he1$1@elna.ethz.ch> NNTP-Posting-Host: b221-gxa300.ethz.ch X-Newsreader: Microsoft Outlook Express 4.72.2106.4 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4 Hi all, can somebody point me to a good (recent?) discussion of the relevance of enzyme pH optimum to cell physiological pH (perhaps especially in bacteria?) what is the current consensus on the topic (if there is one)? Thanks, Stephane ------------------------------------------------------------------- Stephane Vuilleumier Mikrobiologisches Institut ETH-Zurich Tel: (+41) 1 632 33 57 ETH-Zentrum/LFV Fax: (+41) 1 632 11 48 8092 Zurich email: svuilleu@micro.biol.ethz.ch Switzerland http://www.micro.biol.ethz.ch/~svuilleu ------------------------------------------------------------------- From owner-proteins@net.bio.net Tue May 12 23:00:00 1998 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 13 May 1998 02:00:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199805130900.CAA07486@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-proteins@net.bio.net Tue May 12 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!bofh.vszbr.cz!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!dca1-hub1.news.digex.net!digex!news.fas.harvard.edu!oitnews.harvard.edu!news From: Jong Park Newsgroups: bionet.molbio.proteins Subject: How to fix bound 'DNA binding proteins' to DNA? Date: Wed, 13 May 1998 22:02:35 -0400 Organization: Genetics Lines: 15 Message-ID: <355A50BB.2781@salt2.med.harvard.edu> NNTP-Posting-Host: salt2.med.harvard.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; OSF1 V3.2 alpha) Hi, I wonder anybody can tell me how to fix any DNA binding proteins to DNA. Any possible methods are welcome. Crosslinking, increasing affinity by adding some reagents, chemically modifying etc. Any comments are welcome. Thanks very much and I am looking forward to your replies. I appreciate sending me copies of reply to my email address as well. Cheers, Jong From owner-proteins@net.bio.net Tue May 12 23:00:00 1998 Path: biosci!STUDENT1.MAHIDOL.AC.TH!g3937506 From: g3937506@STUDENT1.MAHIDOL.AC.TH (Jongrak Kittiworakarn) Newsgroups: bionet.molbio.proteins Subject: ? Ask for references of Western Blot? Date: 13 May 1998 19:29:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <355A5655.BD10A11C@student.mahidol.ac.th> NNTP-Posting-Host: net.bio.net Hi all: I used TBS buffer and TBS-Tween instead of PBS and PBS-Tween for my Western blotting. I remember that I got this protocols from the RED BOOK, but I do not have any copy of that book here, so I cannot make a check. Anybody can give me any references of the Western Blot protocol that use the mentioned buffer? Thanks you very much in advance. Jongrak -- -----------------------------------Mr. Jongrak Kittiworakarn g3937506@student.mahidol.ac.th Inst. of Science and Technology for Research and Development Mahidol University (Salaya Campus) Salaya, Nokornprathom, THAILAND. Tel. 001-66-2-441-9003-7 ext.1277,1278,1279 Fax. 001-66-2-441-9906, 001-66-2-441-1013 ----------------------------------------------------------------- From owner-proteins@net.bio.net Tue May 12 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!peerfeed.ncal.verio.net!nntp.ni.net!news.service.uci.edu!usenet From: dmandelm@uci.edu (David Mandelman) Newsgroups: bionet.molbio.proteins Subject: DSC data: N2 to 2U Date: 13 May 1998 06:59:49 GMT Organization: UCI Lines: 12 Message-ID: <6jbgd5$o5q@news.service.uci.edu> NNTP-Posting-Host: dialin9072.slip.uci.edu Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 I am interested in finding a mathematical model that can best describe my raw DSC data via nonlinear regression analysis. Preferably, I want a model that can accomodate a dimeric protein undergoing a transition to unfolded monomers. Otherwise, I would appreciate any advice on where I might find more info on DSC data analysis. Thank you in advance, David Mandelman dmandelm@uci.edu From owner-proteins@net.bio.net Tue May 12 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!news-feed.inet.tele.dk!bofh.vszbr.cz!masternews.telia.net!newsfeed.ecrc.net!newscore.univie.ac.at!193.171.255.24.MISMATCH!newsfeed03.univie.ac.at!03-newsfeed.univie.ac.at!fstgal00.tu-graz.ac.at!not-for-mail From: giessauf Newsgroups: bionet.molbio.proteins Subject: Amino Acid Sequences of Lipases/Esterases Date: Wed, 13 May 1998 18:54:07 +0200 Organization: bbb Lines: 41 Message-ID: <3559D02F.4612@glvt.tu-graz.ac.at> Reply-To: bbb NNTP-Posting-Host: fthvtpc11.tu-graz.ac.at Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.0 (Win95; I) Hi, I have a problem to find to amino acid sequenes of following enzymes: -------------------------------------- lipase from Apergillus niger esterase from Horse liver esterase from Mucor miehei esterase from Porcine liver -------------------------------------- I have already tried a search with SWISS-PROT http://expasy.hcuge.ch/ without success. FLUKA sells these enzymes but I was informed by them that they have also performed a literature search without good results (literature till 1990). I have performed a search with Science Citation Index - but it is very time consuming to get all publications to check for the sequences (or to find the references for these sequences) Who can help me to find out if this proteins have already been sequenced ? If you know a good sequence database please inform me or send me one of these sequences by e-mail. Best regards, Andreas Gießauf, PhD Institut für therm.Verfahrenstechnik TU Graz, AUSTRIA e-mail:GIESSAUF@GLVT.TU-GRAZ.AC.AT From owner-proteins@net.bio.net Wed May 13 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!bofh.vszbr.cz!news.maxwell.syr.edu!newsspool.doit.wisc.edu!news.itis.com!news.doit.wisc.edu!default From: klenchin@facstaff.REMOVE_TO_REPLY.wisc.edu (Dima Klenchin) Newsgroups: bionet.molbio.proteins Subject: Re: How to fix bound 'DNA binding proteins' to DNA? Date: Thu, 14 May 1998 18:36:23 GMT Organization: UW-Madison Lines: 17 Message-ID: <6jfdgt$lsq$1@news.doit.wisc.edu> References: <355A50BB.2781@salt2.med.harvard.edu> NNTP-Posting-Host: martinlab.biochem.wisc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=KOI8-R Content-Transfer-Encoding: 8bit X-Newsreader: News Xpress 2.01 X-No-Archive: Yes In article <355A50BB.2781@salt2.med.harvard.edu>, Jong Park wrote: :Hi, : :I wonder anybody can tell me how to fix any DNA binding :proteins to DNA. Any possible methods are welcome. :Crosslinking, increasing affinity by adding some reagents, :chemically modifying etc. Any comments are welcome. : :Thanks very much and I am looking forward to your :replies. :I appreciate sending me copies of reply to my email :address as well. This really depends on what you need it for afterwards. UV should cross-link just fine. - Dima From owner-proteins@net.bio.net Wed May 13 23:00:00 1998 Path: biosci!IBM.RHRZ.UNI-BONN.DE!unb115 From: unb115@IBM.RHRZ.UNI-BONN.DE Newsgroups: bionet.molbio.proteins Subject: congress information Date: 14 May 1998 21:59:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 190 Sender: daemon@net.bio.net Distribution: world Message-ID: <1.5.4.32.19980515045920.0069c7c0@ibm.rhrz.uni-bonn.de> NNTP-Posting-Host: net.bio.net Dear ladies and gentlemen, Dr. Olga Labudov=E1 is organizing the Traditional Meeting on Amino Acids,= the 6th International Congress on Amino Acids, at our department of Experimentelle Radiologie und Strahlenbiologie at the Medical Institutions of the University of Bonn. With this e-mail I enclose the first congress announcement. We would be very obliged if you could distribute these informations to your members. If there are any questions, please do not hesitate to contact Dr. Labudov=E1. For your help we thank you very much in advance. Yours Dr. Olga Labudiv=E1= and Prof. Dr. Rink.=20 6th International Congress on Amino Acids Bonn, Germany, August 3-7th 1999 1st Announcement and call for presentations TOPICS Molecular Medicine, Biochemistry, Nutrition, Pharmaceutics Arginine - Carnitine - Glutamine - Homocysteine - Taurine - Natural Products - Peptides- Modification of Amino Acids - Neurobiology - Neurochemistry - Neurotoxicity - Psychiatry - Physiology - Pharmacology - Toxicology - Microbiology - Cardiology - Nephrology - Pathology - Genetics - Inborn Errors - Molecular Biology - Food Chemistry - Plant Chemistry - Pharmaceutical Chemistry - Radiation and Radical Chemistry Metabolism - Biosynthesis - Analysis - Racemization - Exercise - Memory - Learning. It is our pleasure to invite you to participate in the Traditional Meeting on Amino Acids..The multidisciplinary character of the meeting provides you with an exellent overview of up-to-date knowledge on the role of amino acids in different fields. We will be happy to receive your contribution. Please submit the title of your contribution at your earliest convenience. As the programme shall contain most recent research data, we do not expect an abstract yet. It has to be submitted, however, not later than March 31st, 1999 in order to allow publication prior to the Meeting. The abstracts will be published in the journal AMINO ACIDS, Springer publishers, listed in Current Contents Life Sciences. Please prepare an abstract on a sheet of paper of the size of this letter using 250 words approximately, giving title, authors and adress of corresponding author only. Colleagues who want to submit a full manuscript should ask for the instructions to authors. These are the deadlines: The deadline for the submission of the titles is January 31st, 1999.=20 The deadline for submission of abstracts with or without full manuscripts, for registration and for payment of the congress fee is March 31st, 1999. Early registration is recommended due to a limitation to 600 participants. The registration fee is due latest by March 31st , 1999 preferrably as money order or traveller cheques (cheapest possibilities). The registration form should be completed and submitted to Dr. O. Labudova (address see below). With pleasure we shall satisfy your inquiry on the scientific program and shall send you information on cultural and social events, travelling, housing, and accommodation along with the 2nd announcement, which will be sent to everybody who will return the added registration form. We are looking forward to welcome you and to enjoy your participation as well as your presentation =20 Dr. O. Labudova Rheinische Friedrich-Wilhelms-Universit=E4t Experimentelle Radiologie und Strahlenbiologie, Sigmund-Freud-Strasse 25 D-53105 Bonn, Germany=09 Fax:+49-228-287-4457 e-mail: hrink@mailer.meb.uni-bonn.de Bonn: The Charming Federal City on the Rhine=20 Each year, Bonn welcomes more than two million visitors. There are many ways of reaching this city. One of those is along the Rhine which is the most =84European=93 of all rivers. On its way from the Alps to the North Sea, it flows for 18 kilometers within the boundaries of Bonn accompanied by a wealth of famous sights: castles and ruined fortresses, the romantic Rolandsbogen - the last remaining window arch of a former castle, the Siebengebirge hills, the Federal Government building, the Bonn Opera House and the Beethoven Hall. Even as the German capital, Bonn retained the characteristics that make it what it is today: a medium-sized Rhineland city offering maximum quality of life to those living there and located in one of the loveliest, most romantic regions in Germany. A lot of famous composers found their inspiration in this lovely city, where Ludwig van Beethoven was born and has left his mark. Cologne could be on your list of visits: nowadays, it is the biggest city of the state North-Rhine-Westfalia but already of great importance since the ancient time of the Roman Empire. Cologne is famous for its well-known romanic churches (12) and the gothic cathedral (UNESCO cultural heritage), the Wallraf-Richartz-Museum, the Romano-Germanic archeological museum and many more galleries. D=FCsseldorf one of Germanies fashion cities, often called =84little= Paris=93, is about 1 hour away from Bonn. It is the state capital of North-Rhine Westphalia and administrative headquaters for most of Germanies heavy industry companies. D=FCsseldorf=92s down-town area =84Altstadt=93 and the= shopping district =84K=F6nigsallee=93 are world famous. In addition, D=FCsseldorf=92s= second nickname =84little Tokyo=93, points to the fact that the city houses= Europe=92s largest Japanese colony in Germany. Amsterdam is not far away: it is the famous European North Venice, whose canals in the old city center, the Rijksmuseum and the van Gogh Museum challenge to make an exciting round trip. =20 Brussels the Belgian capital and administrative headquarters of the European Union is about 2,5 hours away from Bonn. Famous for its collection of unique houses from the decorated style and renaissance periods. Brussels today is also famous for its restaurants in all styles found adjacent to =84la= Grande Place=93 - =84Bonne app=E9tit=93.=20 Paris about 4 hours away by train - is always worth a trip. Needless to mention its classical attractions like the Eiffel tower, Notre Dame and the Champs-Elyse=E9s. Le Louvre and the Mus=E9e d=92Orsay are the most famous= examples out of dozens of exceptional museums. Above all, Paris is a unique expression of =84 joie de vivre=93.=20 The 6th International Congress on Amino Acids will be sponsored by: =84Red Bull Company=93=20 Salzburg , Austria. --------------------------------------------------------------------------- ------------------------------------ 6th International Congress on Amino Acids Bonn, Germany, August 3-7th 1999 REGISTRATION FORM I will participate in the congress cited above. Name:______________________________________ Titel: = __________________________ Correct Postal Address:= ________________________________________________________ ___________________________________________________________________________ ZIP: __________________________________ Country: = ____________________________ =09 Phone: _____________________ Fax: _____________________ e-mail: _______________ =09 I will present a contribution =7F Titel: _________________________________________ ___________________________________________________________________________ The fee for early registration (March 31st ,1999) is US dollars 350. Registration fee after March 31st , 1999 is US dollars 380. Fee includes: Printed Abstracts, coffee between sessions, welcome party and conference= dinner. PAYMENT OPTIONS =7F moneyorder available in each office of American Express (charge 12 US= $). =7F enclosed are US $ Traveller cheques or Euro cheques =20 =7F cheque (US $) drawn on a German Bank (add 30 US $ handling charges)=09 Please charge my credit card =7F American Express, =7F Eurocard/Mastercard, =7F Visa Credit card No.: _________________________ Exp. date (valid through) _______________ Name of card holder:= __________________________________________________________ Date and signature := _________________________________________________________ Congress activities will be handled and supported by : American Express Intl., Inc Corporate Services - Business Travel Center Phone: +49-228-7661120 - Fax: +49-228-7661133 From owner-proteins@net.bio.net Wed May 13 23:00:00 1998 Date: Thu, 14 May 1998 20:56:22 +0100 From: gerard@xray.bmc.uu.se (Gerard Kleywegt) Newsgroups: bionet.molbio.proteins Subject: Re: Amino Acid Sequences of Lipases/Esterases Message-ID: References: <3559D02F.4612@glvt.tu-graz.ac.at> Organization: Molecular Biology, Uppsala University X-Newsreader: Value-Added NewsWatcher 2.1d3+ NNTP-Posting-Host: nostromo.bmc.uu.se Lines: 29 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.sprintlink.net!news-backup-west.sprintlink.net!news.sprintlink.net!193.10.88.101!newsfeed.sunet.se!news01.sunet.se!news99.sunet.se!newsfeed.uu.se!nostromo.bmc.uu.se!user In article <3559D02F.4612@glvt.tu-graz.ac.at>, bbb wrote: ;->Hi, ;-> ;-> I have a problem to find to amino acid sequenes ;-> ;-> of following enzymes: ;-> -------------------------------------- ;-> lipase from Apergillus niger ;-> ;-> esterase from Horse liver ;-> ;-> esterase from Mucor miehei ;-> ;-> esterase from Porcine liver ;-> -------------------------------------- ;-> I have already tried a search with SWISS-PROT ;-> there's the ESTHER esterase server in France i don't have the url handy, but a quick altavista search should help --gerard ----------------------------------------------------------- Gerard J. Kleywegt Department of Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, SWEDEN mailto:gerard@xray.bmc.uu.se *** http://alpha2.bmc.uu.se/usf/ From owner-proteins@net.bio.net Wed May 13 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!rill.news.pipex.net!pipex!server1.netnews.ja.net!hgmp.mrc.ac.uk!gmorley From: gmorley@hgmp.mrc.ac.uk (Mr. G. Morley) Newsgroups: bionet.molbio.proteins Subject: looking for a phenotpye Date: 14 May 1998 09:17:58 GMT Organization: MRC Human Genome Mapping Project Resource Centre Lines: 12 Message-ID: <6jecs6$n0g$1@niobium.hgmp.mrc.ac.uk> NNTP-Posting-Host: tin.hgmp.mrc.ac.uk Hello, I was wondering if anyone knows of a human disease which may resemble (in part) the phenotype found for motheaten (me/me) mice.... this would include: Bone abnormalities, over prolifferation of macrophages, possibly some immune disorders. The main symptom would be the large macrophage count. If these symptoms ring any bells I would be gratefull if you could e-mail me at: gmorley@rpms.ac.uk Thanks in advance, Gary Morley. From owner-proteins@net.bio.net Sat May 16 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!wtn-news-feed2.bbnplanet.com!news.bbnplanet.com!news.ums.edu!news.umaryland.edu!umaryland.edu!mremingt From: "Mary P. Remington" Newsgroups: bionet.molbio.proteins Subject: Question about signal sequences. Date: Sun, 17 May 1998 07:33:58 -0400 Organization: University of Maryland, Baltimore Lines: 7 Message-ID: NNTP-Posting-Host: umaryland.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII We are expressing a fusion protein and then overlaying it onto cells. This protein is taken up by the cells but we would like it to be moved inside the cell to the membrane. What signal sequences would target this protein from the cytoplasm to the cell? I have thought of maybe using part of a retrovirus env sequence to target the protein. Any other ideas? Thanks, Mary From owner-proteins@net.bio.net Sat May 16 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gip.net!news.gsl.net!gip.net!portc01.blue.aol.com!audrey01.news.aol.com!not-for-mail From: rick00100@aol.com (Rick00100) Newsgroups: bionet.molbio.proteins Subject: IgG-like proteins in yeast? Lines: 11 Message-ID: <1998051713334400.JAA24540@ladder01.news.aol.com> NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com Date: 17 May 1998 13:33:44 GMT Organization: AOL http://www.aol.com Hi group, I'm doing Western to study genes transformed into yeast. Funny thing was that I got the same banding patterns in transformed and untransformed strains. So the other day I probed my yeast lysate with secondary antibodies only. What do you know, I have the same bands! I tried a few secondary Abs, including monoclonal mouse anti-Rabbit IgG! Guess I'm going to do immunoprecipitation before Western next time, but I'm wondering if anybody has any comment on this? Thanks, Red Lowe From owner-proteins@net.bio.net Sun May 17 23:00:00 1998 Path: biosci!STUDENT1.MAHIDOL.AC.TH!g3937506 From: g3937506@STUDENT1.MAHIDOL.AC.TH (Jongrak Kittiworakarn) Newsgroups: bionet.molbio.proteins Subject: ?Criterion for right conformation? Date: 18 May 1998 08:43:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: <35605631.6D938D3C@student.mahidol.ac.th> NNTP-Posting-Host: net.bio.net Dear all: I have expressed my peptide, a truncated fragment of a protein, in insoluble form, then I refolded it into water soluble peptide. The expressed product was fused to GST. I could not detect any GST activity in the refold protein, but tryptic digestion of the refolded protein gave an trypsin resistance fragment as the tryptic digestion of the native protein. I do not know that the tryptic resistance fragment have the same conformation as the one from the tryptic digestion of the native protein. It was not successful to isolate the trypsin resistance fragment from the native protein under non-denaturing condition ;that is why I have to express this part of peptide. Anyone would please give me general criterion to determind whether the refolded protein adopted the propered conformation? Any suggestion and comment are welcome. Thanks you in advance for your reply. Jongrak -- -----------------------------------Mr. Jongrak Kittiworakarn g3937506@student.mahidol.ac.th Inst. of Science and Technology for Research and Development Mahidol University (Salaya Campus) Salaya, Nokornprathom, THAILAND. Tel. 001-66-2-441-9003-7 ext.1277,1278,1279 Fax. 001-66-2-441-9906, 001-66-2-441-1013 ----------------------------------------------------------------- From owner-proteins@net.bio.net Sun May 17 23:00:00 1998 Path: biosci!STUDENT1.MAHIDOL.AC.TH!g3937506 From: g3937506@STUDENT1.MAHIDOL.AC.TH (Jongrak Kittiworakarn) Newsgroups: bionet.molbio.proteins Subject: ? Denaturants used in refold? Date: 18 May 1998 08:19:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <35605085.6492E1A8@student.mahidol.ac.th> NNTP-Posting-Host: net.bio.net Dear all: Are there anyone who have heard about protocol that using more than one kind of denaturing agent together to unfold and refold of protein? Thanks you in advanace for any infomation. Jongrak -- -----------------------------------Mr. Jongrak Kittiworakarn g3937506@student.mahidol.ac.th Inst. of Science and Technology for Research and Development Mahidol University (Salaya Campus) Salaya, Nokornprathom, THAILAND. Tel. 001-66-2-441-9003-7 ext.1277,1278,1279 Fax. 001-66-2-441-9906, 001-66-2-441-1013 ----------------------------------------------------------------- From owner-proteins@net.bio.net Mon May 18 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!128.174.5.49!vixen.cso.uiuc.edu!uchinews!news.luc.edu!not-for-mail From: tmitch1@orion.it.luc.edu (tmitch1) Newsgroups: bionet.molbio.proteins Subject: GSH problem Date: 19 May 1998 17:36:04 GMT Organization: Loyola University Chicago Lines: 11 Message-ID: <6jsfu4$bkn$1@artemis.it.luc.edu> Reply-To: tmitch1@orion.it.luc.edu NNTP-Posting-Host: 147.126.26.5 X-Newsreader: WinVN 0.92.6+ Hello. I was wondering if anyone has used a glutathione analog in which the sulfhydral group is blocked to elude bound GST-fusion proteins from a GSH-Sepharose Chromatography Column (Pharmacia). Reduced glutathione (GSH) is currently used, but it binds to our peptides via two disulfide bonds to two cysteines in our target peptide. DTT removes the GSH, but it produces the target peptide with reduced cysteine residues. We suspect that the cysteines form a native disulfide bond in the absence of GSH and DTT. Any suggestions? Thanks for your time. Tracy Mitchell Loyola University of Chicago tmitch1@orion.it.luc.edu From owner-proteins@net.bio.net Tue May 19 23:00:00 1998 From: Robert Alexander Reiprich Newsgroups: bionet.molbio.proteins Subject: somatostatin Date: Wed, 20 May 1998 16:03:52 +0200 Organization: institute of neurophysiology Lines: 12 Message-ID: <3562E2C8.2565F132@uni-duesseldorf.de> NNTP-Posting-Host: 134.99.144.84 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.01 [de] (Win95; I) X-Priority: 3 (Normal) Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!news-feed.inet.tele.dk!bofh.vszbr.cz!fu-berlin.de!news.CS.Uni-Magdeburg.De!RRZ.Uni-Koeln.DE!news.rz.uni-duesseldorf.de!usenet Hi out there! Is anybody in this group working with somatostatin analogues specifically acting on the SSTR-1, SSTR-2 and/or SSTR-5? I investigate the effects of these peptides on synaptic transmission in the neocortex of the rat. Therefore I am searching for labs who synthesize these peptides for their own studies. I would be interested in buying small amounts of high affinity agonists especially for the SSTR-1 and SSTR-5 receptors. Any help is wellcome! Alexander Reiprich From owner-proteins@net.bio.net Tue May 19 23:00:00 1998 Path: biosci!news.Stanford.EDU!newsfeed.concentric.net!netnews.com!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!baron.netcom.net.uk!netcom.net.uk!server3.netnews.ja.net!news.ox.ac.uk!not-for-mail From: Leanne Allhouse Newsgroups: bionet.molbio.proteins Subject: DE-52 Columns Date: Wed, 20 May 1998 12:51:58 +0100 Organization: University Laboratory of Physiology, Oxford Lines: 7 Message-ID: <3562C3DE.6B614B21@physiol.ox.ac.uk> NNTP-Posting-Host: pc-laser6.physiol.ox.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.05 [en] (Win95; I) I was once shown how to purify barnacle tnc proteins using DE-52 columns using the buffer 6M urea, 50mM Tris-HCl, 1mM EDTA,1mM DTT but was wondering if anyone can tell me why it is important to measure the conductivity of the column buffer, loaded sample and eluted fractions? Thanks Leanne From owner-proteins@net.bio.net Tue May 19 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!dallas-news-feed2.bbnplanet.com!news.bbnplanet.com!newsfeed.gte.net!nntp.giganews.com!uunet!in2.uu.net!hearst.acc.Virginia.EDU!aruba.odu.edu!news From: Laura Moen Newsgroups: bionet.molbio.proteins Subject: Re: GSH problem Date: Wed, 20 May 1998 12:25:26 -0400 Organization: Old Dominion Universityaruba Lines: 25 Message-ID: <356303F6.FB4017A6@odu.edu> References: <6jsfu4$bkn$1@artemis.it.luc.edu> NNTP-Posting-Host: viper229132.sci1.odu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.04 [en] (WinNT; I) Tracy, Is your protein too large to fold correctly if you simply let the Sulfhydryls oxidize after removing the DTT by dialysis? I would try that. I would also try the analog in case that works better. (I assume you have one all picked out - I don't know of any off-hand.) Alternatively, can you change your construct to a His tag instead of the GST fusion and avoid the sulfhydryl problem that way? Good luck. Laura tmitch1 wrote: > Hello. I was wondering if anyone has used a glutathione analog in which the sulfhydral group > is blocked to elude bound GST-fusion proteins from a GSH-Sepharose Chromatography Column > (Pharmacia). Reduced glutathione (GSH) is currently used, but it binds to our peptides > via two disulfide bonds to two cysteines in our target peptide. DTT removes the GSH, but it > produces the target peptide with reduced cysteine residues. We suspect that the cysteines > form a native disulfide bond in the absence of GSH and DTT. Any suggestions? Thanks for your > time. > > Tracy Mitchell > Loyola University of Chicago > tmitch1@orion.it.luc.edu From owner-proteins@net.bio.net Tue May 19 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsxfer3.itd.umich.edu!nntp.news.xara.net!xara.net!server5.netnews.ja.net!server3.netnews.ja.net!server1.netnews.ja.net!warwick!leicester!usenet From: "Dr E. Buxbaum" Newsgroups: bionet.molbio.proteins Subject: Re: ?Criterion for right conformation? Date: Wed, 20 May 1998 17:05:02 -0700 Organization: University of Leicester (PCFS User) Lines: 10 Message-ID: <35636FAE.660C@le.ac.uk> References: <35605631.6D938D3C@student.mahidol.ac.th> NNTP-Posting-Host: pc10.msb.le.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win16; I) Jongrak Kittiworakarn wrote: > I have expressed my peptide, a truncated fragment of a protein, in > insoluble form, then I refolded it into water soluble peptide. > Anyone would please give me general criterion to determind whether the > refolded protein > adopted the propered conformation? 2D NMR would be the most rigorous proof. You'll have to get into contact with somebody who has the equipment and expertise to do this. From owner-proteins@net.bio.net Tue May 19 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.new-york.net!uunet!in2.uu.net!hearst.acc.Virginia.EDU!aruba.odu.edu!news From: Laura Moen Newsgroups: bionet.molbio.proteins Subject: Re: DE-52 Columns Date: Wed, 20 May 1998 12:30:49 -0400 Organization: Old Dominion Universityaruba Lines: 24 Message-ID: <35630539.21DC6559@odu.edu> References: <3562C3DE.6B614B21@physiol.ox.ac.uk> NNTP-Posting-Host: viper229132.sci1.odu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.04 [en] (WinNT; I) Leanne, DE-52 is an anion exchange resin. Like other ion exchange resins, that means that ionic strength is an important factor in protein interactions with the column matrix. If you want to know more about the use of ion exchange resins in protein purification and other things, you might look in Scopes book on Protein Purification. Also, I suspect other individuals in the laboratory where you are working can be of assistance. And, there may even be a technical information sheet from the manufacturer of the resin which can provide additional info. Hope this helps. Laura Leanne Allhouse wrote: > I was once shown how to purify barnacle tnc proteins using DE-52 columns > using the buffer 6M urea, 50mM Tris-HCl, 1mM EDTA,1mM DTT but was > wondering if anyone can tell me why it is important to measure the > conductivity of the column buffer, loaded sample and eluted fractions? > > Thanks > Leanne From owner-proteins@net.bio.net Wed May 20 23:00:00 1998 From: "Carsten Hohoff" Newsgroups: bionet.molbio.proteins Subject: SP antibodies? Date: Thu, 21 May 1998 17:19:24 +0200 Organization: Westfaelische Wilhelms-Universitaet Muenster, Germany Lines: 12 Sender: "Carsten Hohoff" Message-ID: NNTP-Posting-Host: asterix.uni-muenster.de Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII Path: biosci!agate!howland.erols.net!newsfeed.nacamar.de!wuff.mayn.de!news.CS.Uni-Magdeburg.De!RRZ.Uni-Koeln.DE!news-koe1.dfn.de!uni-muenster.de!asterix.uni-muenster.de!hohoff Dear Netters, I am looking for antibodies against surfactant-associated proteins (SP-A, SP-B, SP-C, SP-D) as markers for type II pneumocytes in lung sections. Are these antibodies commercially available? I checked several catalogues and web pages, but did not succeed at all... Thanx in advance Carsten Hohoff From owner-proteins@net.bio.net Wed May 20 23:00:00 1998 Path: biosci!bloom-beacon.mit.edu!news.kodak.com!news-nysernet-16.sprintlink.net!207.41.200.132!news-pen-2.sprintlink.net!news-pen-1.sprintlink.net!news.sprintlink.net!newsfeed1.swip.net!masternews.telia.net!Cabal.CESspool!bofh.vszbr.cz!newscore.univie.ac.at!193.171.255.24.MISMATCH!newsfeed03.univie.ac.at!03-newsfeed.univie.ac.at!news.univie.ac.at!not-for-mail From: "Dietmar Winkler" Newsgroups: bionet.molbio.proteins Subject: 5-HT1-Receptors Date: Thu, 21 May 1998 22:47:37 +0200 Organization: Vienna University, Austria Lines: 12 Message-ID: <6k24lq$16deo$1@www.univie.ac.at> NNTP-Posting-Host: uvo-62.univie.ac.at X-Newsreader: Microsoft Outlook Express 4.72.2106.4 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4 Hi! Does anybody know where to find 3d-images of 5-HT1-Receptors (5-HT1A-F) on the internet? All help on this would be greatly appreciated! Thanks in advance, Dietmar. From owner-proteins@net.bio.net Wed May 20 23:00:00 1998 Path: biosci!fcs280s.ncifcrf.gov!wtn-news-feed2.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newshub.northeast.verio.net!news.pn.com!nntp.pn.com!uunet!in2.uu.net!hearst.acc.Virginia.EDU!aruba.odu.edu!news From: Laura Moen Newsgroups: bionet.molbio.proteins Subject: Re: somatostatin Date: Thu, 21 May 1998 12:47:48 -0400 Organization: Old Dominion Universityaruba Lines: 23 Message-ID: <35645AB4.E49B5FD5@odu.edu> References: <3562E2C8.2565F132@uni-duesseldorf.de> NNTP-Posting-Host: viper229132.sci1.odu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.04 [en] (WinNT; I) Alexander, Have you looked in the Bachem catalog? They have stocks of peptides that are frequently used (i.e.like pepstatin) and they also do custom peptide synthesis. Laura Robert Alexander Reiprich wrote: > Hi out there! > > Is anybody in this group working with somatostatin analogues > specifically acting on the SSTR-1, SSTR-2 and/or SSTR-5? I investigate > the effects of these peptides on synaptic transmission in the neocortex > of the rat. Therefore I am searching for labs who synthesize these > peptides for their own studies. I would be interested in buying small > amounts of high affinity agonists especially for the SSTR-1 and SSTR-5 > receptors. > Any help is wellcome! > Alexander Reiprich From owner-proteins@net.bio.net Sat May 23 23:00:00 1998 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!208.134.241.18!newsfeed.internetmci.com!206.229.87.25!news-peer.sprintlink.net!news-backup-east.sprintlink.net!news.sprintlink.net!204.251.80.3!mercury.galstar.com!news.okstate.edu!lse405.cas.okstate.edu!user From: burnap@biochem.okstate.edu (Robert L. Burnap) Newsgroups: bionet.molbio.proteins Subject: postdoctoral position Date: 22 May 1998 14:22:49 GMT Organization: Oklahoma State University Lines: 28 Message-ID: NNTP-Posting-Host: lse405.cas.okstate.edu POSTDOCTORAL RESEARCH ASSOCIATE POSITION Molecular Analysis of Photosynthetic Complexes An NSF-supported postdoctoral position is available immediately to study basic protein structure-function relationships of the photosystem II reaction center complex using the experimental model Synechocystis 6803. The work combines state-of-the-art molecular genetic, biochemical, and biophysical approaches to clarify the activity and light-dependent assembly of the catalytic tetramer of manganese atoms that forms the core of the H2O-splitting enzyme. A Ph.D. and a strong background in protein biochemistry and/or molecular biology is required. Preferences will be given to individuals with proven records of quality publications and to those with potentials to obtain independent funding. Please send CV along with the names, addresses, and telephone numbers of three referees to: Dr. Robert L. Burnap, Microbiology & Molecular Genetics Life Sciences East Oklahoma State University, Stillwater, Oklahoma 74078 e-mail: burnap@biochem.okstate.edu; http://www.cas.okstate.edu/micro/faculty/BURNAP/index.html. Equal opportunity employer/AA From owner-proteins@net.bio.net Sat May 23 23:00:00 1998 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!207.97.14.174!europa.clark.net!199.60.229.5!newsfeed.direct.ca!torn!nott!crc-news.crc.ca!srv1.drenet.dnd.ca!newshost.dres.dnd.ca!poirier.dres.dnd.ca!bpoirier From: bpoirier@dres.dnd.ca (bob poirier) Newsgroups: bionet.molbio.proteins Subject: on- or precolumn concentration of proteins Date: Fri, 22 May 1998 16:46:01 GMT Organization: DRES Lines: 3 Message-ID: NNTP-Posting-Host: poirier.dres.dnd.ca X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Has anyone seen any recent Capillary Electrophoresis articles ( 1997-98) on 'online or precolumn concentration of proteins'. Found about 11 but could use more. From owner-proteins@net.bio.net Sun May 24 23:00:00 1998 Path: biosci!POST.ITS.MCW.EDU!ferreira From: ferreira@POST.ITS.MCW.EDU (Paulo Ferreira) Newsgroups: bionet.molbio.proteins Subject: Postdoctoral and Research Assistant Positions Date: 25 May 1998 11:10:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 66 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.1.32.19980525125850.0082a750@post.its.mcw.edu> NNTP-Posting-Host: net.bio.net Applications for postdoctoral and research assistant positions are= immediately available in my laboratory. Candidates should be highly= motivated and have good expertise in molecular biology, biochemistry and/or= cell biology techniques. Candidates with experience in yeast two-hybrid= system and/or general protein chemistry are particularly attractive. Current work underway in the laboratory focus on studying biogenic= steps of G- protein coupled receptors and phosphoinositide signaling in= neuronal cells. We have been particularly interested in investigating i)= the molecular mechanisms underlying the function of a new neuronal= signaling pathway mediated by a novel PI-phospholipase C (e.g. Ferreira, P.= et al. (1994). J. Biol. Chem., 269, 3129; Ferreira, P. et al. (1993) Proc.= Natl. Acad. Sci. USA, 90, 6042) and, ii) the molecular machinery involved= in the intracellular processing and trafficking of homologous G-protein= coupled receptors in highly polarized neuronal cells (e.g. Ferreira et al.= (1998), J. Biol. Chem. submitted; Ferreira, P. et al. (1997) Proc. Natl.= Acad. Sci. USA, 94, 1556; Ferreira, P. et al. (1996) Nature 383, 637;= Ferreira, P. et al. (1995) J. Biol. Chem. 270, 23179). In addition, we are= using our studies as a model system to study widespread homologous= molecular mechanisms in other biological systems and understand the= molecular pathogenesis underlying certain inherited neurological disorders= in humans which ultimately lead to the degeneration of selective neurons in= the CNS. The laboratory and institution are equipped with state-of-art= facilities and operate in an outstanding research environment. The= prospective candidate is expected to participate in campus-wide interactive= programs such as seminars and ultimately, present his\her research results= at international meetings and publish in top-rated scientific journals. = Salary is based on experience and NIH guidelines. Candidates with an overall interest in neurobiology are invited to= apply and should send their CV, summary of their past research experience= and future goals, and three reference letters directly to: Paulo A. Ferreira, Ph.D. Medical College of Wisconsin Pharmacology Department 8701 Watertown Plank Road Milwaukke, WI 53226 Phone: 414-456-8877 Fax: 414-456-6545; =20 Email: ferreira@post.its.mcw.edu 0000,0000,8080Paulo A. Ferreira, Ph.D. Assistant Professor 8080,0000,0000Medical College of Wisconsin Pharmacology Department 8701 Watertown Plank Road Milwaukee, WI 53226 FAX: 414-456-6370 // -6545=20 Phone: 414-456-8877 // -8267; Lab: -8043 From owner-proteins@net.bio.net Sun May 24 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ix.netcom.com!news From: Mark Atlas Newsgroups: bionet.molbio.proteins Subject: Online Auctions for used lab equipment with Going, Going...Sold! Date: Mon, 25 May 1998 14:27:45 GMT Organization: ICGNetcom Lines: 66 Message-ID: <6kbvap$e20@sjx-ixn9.ix.netcom.com> NNTP-Posting-Host: sjx-ca115-61.ix.netcom.com X-NETCOM-Date: Mon May 25 7:30:49 AM PDT 1998 X-Newsreader: NETCOMplete/4.0 Online Auctions for used lab equipment with Going, Going...Sold! Going, Going...Sold has rapidly become the fastest growing Internet source for high quality used lab equipment. All equipment providers as prescreened and evaluated for integrity prior to placement of their listing. All purchase utilize the Going, Going Sold! in lab evlauation period before you turn money over to the seller. Recent new services include Instrument lease to own programs and a rapidly growing equipment search service for items not on our site. The list is growing weekly , Please visit us @ http://www.going-going-sold.com The following Items are scheduled to go on Auction over the next few weeks. ***************************************************** Sale Closing : 5/29/98 Opening Prices ***************************************************** 1. automatic abbe refractometer $2,000.00 2. Waters 717 autosampler $4,700.00 3. Environmental Chamber $5,250.00 4. ICP JY-138 Ultrace $45,000.00 5. Graphite Furnace AA 5100ZL $24,000.00 6. Microwave Digestor 1000W $7,000.00 7. Microwave Digestor $1,800.00 8. BOD Incubator $2,300.00 9. Laminar Flow Hood $1,900.00 10. 4' Fume hood $2,000.00 11. Phase Contrast Microscope $2,000.00 ***************************************************** Sale Closing : 6/3/98 Opening Prices ***************************************************** 1. Sorvall RC 3B and Rotor $7,400.00 2. Centra 4B $1,395.00 3. Gilson HPLC System $5,450.00 4. Model 996 PDA Detector $4,750.00 5. Waters GPC system $10,500.00 6. Capillary Rheometer System $4,750.00 7. Inductively Coupled Plasma $19,400.00 8. Polarizing Microscope $1,900.00 9. Leica Microscope $1,500.00 ***************************************************** Sale Closing : 6/5/98 Opening Prices ***************************************************** 1. Tangential Flow System $15,000.00 2. Beckman GPR Centrifuge $3,100.00 3. Beckman L5-75 Centrifuge& Rotors $6,900.00 4. Beckman DU-62 $4,200.00 5. Beta Scint. Counter $13,000.00 6. Beckman DU-70 $4,900.00 7. Bio-safety hood 4 foot with stand Type IIA, or IIB $2,200.00 8. Oligo 1000 $1,750.00 9. Fluoresence Microscope $4,800.00 ***************************************************** Sale Closing : 6/10/98 Opening Prices ***************************************************** 1. Beckman L5-50 Centrifuge $3,400.00 2. Beckman L7-65 Ultra Centrifuge $4,850.00 3. Beckman TJ-6R Centrifuge $2,250.00 4. Purifier Class II $2,500.00 5. Amino Acid Analyzer $35,000.00 6. Tekmar 2000 sampler $6,900.00 7. Liquid Chromatograph $27,900.00 8. Optima Ultracentrifuge $20,000.00 ***************************************************** © 1997, Internet Auctioneers International From owner-proteins@net.bio.net Sun May 24 23:00:00 1998 Path: biosci!agate!logbridge.uoregon.edu!news-peer.gip.net!news.gsl.net!gip.net!newsfeed.internetmci.com!193.174.75.110!news-was.dfn.de!news-fra1.dfn.de!news-koe1.dfn.de!news-han1.dfn.de!news.gwdg.de!not-for-mail From: Silke Beismann Newsgroups: bionet.molbio.proteins Subject: IGP and glutamine Date: Mon, 25 May 1998 10:43:30 +0200 Organization: GWDG, Goettingen Lines: 10 Message-ID: <35692F32.59D7@Uni-MolGen.gwdg.de> NNTP-Posting-Host: 134.76.70.64 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (WinNT; I) Hi, I work on a glutaminase assay, and to optimize the assay I want to find out two things: 1. Does anyone know if IGP (imidazole glycerol phosphat) is stable in aquaous solution ? 2. What is the intracellular level of glutamine in E. coli? Thanks for your answers! Silke From owner-proteins@net.bio.net Sun May 24 23:00:00 1998 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!128.230.129.106!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: busygin@a-teleport.com Newsgroups: bionet.molbio.proteins Subject: Please help me Date: Mon, 25 May 1998 01:04:49 GMT Organization: Deja News - The Leader in Internet Discussion Lines: 15 Message-ID: <6kag3h$39u$1@nnrp1.dejanews.com> NNTP-Posting-Host: 194.44.23.65 X-Article-Creation-Date: Mon May 25 01:04:49 1998 GMT X-Http-User-Agent: Mozilla/2.0 (compatible; MSIE 3.01; Windows 95) X-Http-Proxy: 1.0 proxy.alkar.net:8080 (Squid/1.1.15) for client 194.44.23.74 Hello, Excuse me for this non-professional question but I'm not a biologist. As far as I know Protein Prediction Structure Problem is NP-complete. I have some new technique for solving NP-hard problem and I want to try it on PSP, but I don't know math statement for the problem. Could you send me it? Thank you in advance, Stas Busygin email: busygin@a-teleport.com NP-Completeness Webpage: http://www.busygin.dp.ua/npc.html -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/ Now offering spam-free web-based newsreading From owner-proteins@net.bio.net Sun May 24 23:00:00 1998 From: Cornelius Krasel Subject: Re: 5-HT1-Receptors Newsgroups: bionet.molbio.proteins References: <6k24lq$16deo$1@www.univie.ac.at> User-Agent: tin/pre-1.4-980226 (UNIX) (Linux/2.0.33 (i486)) Date: Sun, 24 May 1998 19:17:43 +0200 Message-ID: NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de Lines: 14 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!207.97.14.174!europa.clark.net!194.162.162.196!newsfeed.nacamar.de!chico.franken.de!uni-erlangen.de!uni-wuerzburg.de!not-for-mail Dietmar Winkler wrote: > Does anybody know where to find 3d-images > of 5-HT1-Receptors (5-HT1A-F) on the internet? Possibly from http://www.sander.embl-heidelberg.de/7tm/models/ --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP4 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-proteins@net.bio.net Sun May 24 23:00:00 1998 Path: biosci!sfu.ca!inorthwo From: inorthwo@sfu.ca (ingrid northwood) Newsgroups: bionet.molbio.proteins Subject: Protease specificity database Date: 25 May 1998 14:06:48 -0700 Organization: Simon Fraser University Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <3569DCEB.6E42@sfu.ca> Reply-To: inorthwo@sfu.ca NNTP-Posting-Host: net.bio.net I am wondering if anyone can help me locate a database of amino acid sequences and the corresponding protease that cuts at that sequence? We are trying to design peptide linkers for various proteases and are trying to come up with the best target we can. Thanks in advance for any help, Rob Linning (rlinning@sfu.ca). From owner-proteins@net.bio.net Mon May 25 23:00:00 1998 Newsgroups: bionet.molbio.proteins Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ais.net!vixen.cso.uiuc.edu!uchinews!midway.uchicago.edu!aekentsi From: aekentsi@midway.uchicago.edu (alex kentsis) Subject: ES-MS on small proteins X-Nntp-Posting-Host: howard-nfs.uchicago.edu Message-ID: Sender: news@midway.uchicago.edu (News Administrator) Organization: The University of Chicago X-Newsreader: TIN [version 1.2 PL2] Date: Tue, 26 May 1998 13:21:06 GMT Lines: 8 i am trying to use an electrospray-mass spectrometer with 9-14 kD proteins, but am getting severe attenuation at the detector. can someone recommend a buffer system to increase the signal:noise? From owner-proteins@net.bio.net Tue May 26 23:00:00 1998 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!rill.news.pipex.net!pipex!server1.netnews.ja.net!news.nott.ac.uk!pmbfjd0.nottingham.ac.uk!user From: mbzrl@mbn1.biochem.nottingham.ac.uk (Rob) Newsgroups: bionet.molbio.proteins Subject: Mono-Q/ATP Followup-To: bionet.molbio.proteins Date: 27 May 1998 14:06:08 GMT Organization: University of Nottingham Lines: 7 Message-ID: NNTP-Posting-Host: pmbfjd0.nottingham.ac.uk Hi Does anyone have experience of using ATP-containing buffers with Mono-Q (or equivalent) FPLC columns. In particular, am I correct in assuming that ATP binds the column and comes off as a peak around 0.15-0.20M NaCl (in 50mM Tris pH7.5)? Thanks in advance Rob From owner-proteins@net.bio.net Tue May 26 23:00:00 1998 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed4.bbnplanet.com!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news1.bellglobal.com!garnet.nbnet.nb.ca!news.unb.ca!coranto.ucs.mun.ca!a69rlt From: a69rlt@morgan.ucs.mun.ca (Robin Lee Tremblay) Newsgroups: bionet.molbio.proteins Subject: RNA oligos Date: 27 May 1998 14:28:36 GMT Organization: Memorial University of Newfoundland Lines: 24 Message-ID: <6kh7uk$mvu$1@coranto.ucs.mun.ca> NNTP-Posting-Host: plato.ucs.mun.ca X-Newsreader: TIN [version 1.2 PL2] Has anyone heard of a place where you can get RNA oligos synthesized? Is this even possible? I'd like to do some electrophoretic mobility shift assays using RNA instead of DNA, but would rahter not have to try and isolate and purify a specific RNA myself. I was also wondering if anyone had any tried and true protocols for the EMSA - I'm trying to decide on an approach for the experiment. Thanks in advance Robin -- *************************************************************************** | Robin Tremblay | ooo ooo a69rlt@morgan.ucs.mun.ca | o o o o Department of Biochemistry | ooo ooo Memorial University of Newfoundland | \ / (709)-737-2538 | \ / | ------- *************************************************************************** From owner-proteins@net.bio.net Wed May 27 23:00:00 1998 Path: biosci!news.Stanford.EDU!newsfeed.concentric.net!newsfeed1.earthlink.net!news.maxwell.syr.edu!nntp.news.xara.net!xara.net!server6.netnews.ja.net!server1.netnews.ja.net!news.nott.ac.uk!pdxkgs From: pdxkgs@pdn1.gene.nott.ac.uk (Karen spink) Newsgroups: bionet.molbio.proteins Subject: eluting proteins from gels Date: Thu, 28 May 1998 17:59:01 +0100 Organization: University of Nottingham Lines: 13 Message-ID: NNTP-Posting-Host: ppdirg.nottingham.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Yet Another NewsWatcher 2.4.0 Recently, i have been dabbling in a bit of protein purification and on a coomasie gel i have only a few proteins, one in particular is stronger than the others. I wish to show that this stronger band is the protein responsible for activity I am detecting in the fraction. Is it feasible to simply elute this band from the SDS gel and assay this for activity, i.e. is there a simple way of eluting the protein directly from the gel with a good chance of renaturing the protein? Thanks in anticipation Karen Spink E. mail pdxkgs@pdn1.gene.nottingham.ac.uk From owner-proteins@net.bio.net Wed May 27 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.idt.net!WCG!news.airnews.net!cabal12.airnews.net!news.airnews.net!cabal10.airnews.net!news-feeder.onramp.net!news.onramp.net!not-for-mail From: "Cynthia S. Smagula" Newsgroups: bionet.molbio.proteins Subject: 100 New Tools Added to BioToolKIt Date: Thu, 28 May 1998 11:46:13 +0000 Organization: BIOTA Publications Lines: 29 Message-ID: <356D4E82.37C6@onramp.net> Reply-To: biota@onramp.net NNTP-Posting-Host: isdn12-49.dllstx.onramp.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.04 (Macintosh; I; PPC) The BioToolKit, an annotated directory of over 600 online molecular biology resources (at http://www.biosupplynet.com) has recently added links to many new resources, including: ACUTS (Ancient Conserved Untranslated Sequences) dbCFC (Cytokine Family database) DNA Patent Database CD Guides (cell surface markers) MAR-Finder (Matrix Attachment Regions) In Situ PCR on Plant Material Vacuum Infiltration Transformation of Arabidopsis PPMdb (Plant Plasma Membrane database) MitBASE (comprehensive mitochondrial database) GenProtEC (E.coli gene relationships) The Interactive Fly WormPep (predicted proteins in C.elegans) WebMolecules VRML Player (analyzes your system, suggests viewers) 3-D Crunch (SWISS-MODEL predicts 50,000 structures) Atlas of Protein Topology Cartoons (simple 2-D diagrams) Movies of Protein Motions Protein Morphing Server PROMISE: Prosthetic Groups and Metal Ions in Protein Active Sites ADOPS: Associative Database of Protein Sequences NeuronDB Complete descriptions of these sites and links are found at http://www.biosupplynet.com, just click on the BioToolKit. The BioToolKit database is designed for rapid page delivery, functionally organized, and Verity-searchable. From owner-proteins@net.bio.net Wed May 27 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!164.67.42.145!awabi.library.ucla.edu!164.67.80.81!news.ucla.edu!not-for-mail From: Laurence Lavelle Newsgroups: bionet.molbio.proteins Subject: Re: RNA oligos Date: Thu, 28 May 1998 16:36:37 -0700 Organization: University of California, Los Angeles Lines: 38 Message-ID: <356DF505.348F384F@mbi.ucla.edu> References: <6kh7uk$mvu$1@coranto.ucs.mun.ca> Reply-To: lavelle@mbi.ucla.edu NNTP-Posting-Host: red5.mbi.ucla.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.01 [en] (WinNT; U) To: Robin Lee Tremblay X-Priority: 3 (Normal) I am also looking for a source of synthesized & pure RNA oligo's Thanks Laurence Robin Lee Tremblay wrote: > Has anyone heard of a place where you can get RNA oligos synthesized? > Is > this even possible? I'd like to do some electrophoretic mobility shift > > assays using RNA instead of DNA, but would rahter not have to try and > isolate and purify a specific RNA myself. > > I was also wondering if anyone had any tried and true protocols for > the > EMSA - I'm trying to decide on an approach for the experiment. > > Thanks in advance > > Robin > > -- > ** > ************************************************************************ > > | > Robin Tremblay | ooo ooo > a69rlt@morgan.ucs.mun.ca | o o o o > Department of Biochemistry | ooo ooo > Memorial University of Newfoundland | \ / > (709)-737-2538 | \ / > | ------- > ********************************************************* > ***************** From owner-proteins@net.bio.net Thu May 28 23:00:00 1998 Path: biosci!cardiff.ac.uk!JohnsonRJ From: JohnsonRJ@cardiff.ac.uk (Rowena Johnson) Newsgroups: bionet.molbio.proteins Subject: nuclear protein isolation Date: 29 May 1998 05:03:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <229CDE614D0@wmcu2.uwcm.ac.uk> NNTP-Posting-Host: net.bio.net Hello, I want to isolate nuclear proteins from whole tissue (specifically heart muscle) but without any major disruption. I am used to making nuclear extracts from cell cultures by a modified version of Dignam et al but have not been able to find a technique to isolate nuclear proteins from whole tissue. In particular I want to isolate NF-kB which is very sensitive to stress and am therefore looking for the most gentle method available for this. I would be most grateful to anyone who has any ideas on the matter, Rowena Johnson Dr Rowena Johnson Department of Cardiology, Tenovus Building, University of Wales College of Medicine, Heath Park, Cardiff. CF4 4XX Phone: 01222 744518 From owner-proteins@net.bio.net Thu May 28 23:00:00 1998 Path: biosci!agate!howland.erols.net!newsfeed.internetmci.com!192.220.250.21!netnews1.nw.verio.net!netnews.nwnet.net!news.wa-k20.net!news.wsu.edu!cheetah.it.wsu.edu!kwon1 From: Young Ho Kwon Newsgroups: bionet.molbio.proteins Subject: Re: RNA oligos Date: Fri, 29 May 1998 00:07:35 -0700 Organization: Washington State University Lines: 12 Message-ID: References: <6kh7uk$mvu$1@coranto.ucs.mun.ca> <356DF505.348F384F@mbi.ucla.edu> NNTP-Posting-Host: cheetah.it.wsu.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: kwon1@cheetah.it.wsu.edu To: Laurence Lavelle cc: Robin Lee Tremblay In-Reply-To: <356DF505.348F384F@mbi.ucla.edu> Keck Oligonucleotide Synthesis Facility may synthesize your oligo-RNA. e-mail address : oligos@yale.edu http://info.med.yale.edu/wmkeck/oligos.htm tel : 203-737-2069 Good luck. YoungHo Biochemistry/Biophysics Washington State University From owner-proteins@net.bio.net Thu May 28 23:00:00 1998 Path: biosci!acdlabs.com!vic From: vic@acdlabs.com ("Victor Graziano") Newsgroups: bionet.molbio.proteins Subject: Protein Manager Free Software Demo Date: 29 May 1998 12:25:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Distribution: world Message-ID: <000701bd8b50$6b40df20$3ee9b0cf@vic.acdlabs.com> NNTP-Posting-Host: net.bio.net Dear Colleagues Advanced Chemistry Development Inc. (ACD), a leader in Cheminformatics software, has just released Protein Manager, the newest member of its extensive software family. This Bioinformatics software package, coupled with the Swissprot, Prosite, PDB, ACD Restriction Enzyme and (soon to follow) ACD Regulatory Protein Databases, will dramatically accelerate protein research and peptide drug discovery. ACD/Protein Manager is built around a three-in-one functionality concept which provides all the necessary features to Analyze, Publish (Web & Desktop) and Manage protein sequence data. Software operation is easy, simply load candidate proteins into the Protein Manager 'protein workbook' and perform a multitude of functions including: -Isoelectric point determination -Batch Phys-Chem property predictions -Protein secondary structure prediction (including the newest Predator prediction method) -Multiple protein alignments with consensus matching -Enzymatic digestion of selected proteins (utilizing ACD's enzyme database) -Protein scale determination (hydrophobicity, polarity, bulkiness...) -3D viewing of all available Protein Database (PDB) files More information outlining Protein Manager's functionality, along with a product demo, short video and future software releases (ACD Regulatory peptide DB or ACD/Gene Manager) are available at: http://www.acdlabs.com/products/peptide/prot_mgr.html. Please feel free to contact myself or any of the other ACD representatives for further information regarding Protein Manager or any of the other ACD software titles. Regards, Victor Graziano B.Sc., M.Sc. Advanced Chemistry Development, Inc. Account Manager (Biochemistry) T: (416) 368-3435 F: (416) 368-5596 US & Canada: (800) 304-3988 http://www.acdlabs.com From owner-proteins@net.bio.net Sat May 30 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!news.mel.connect.com.au!munnari.OZ.AU!metro!not-for-mail From: xiuhong wang Newsgroups: bionet.molbio.proteins Subject: chymotrypsin Date: Mon, 01 Jun 1998 15:55:47 +0000 Organization: University of Sydney Lines: 5 Message-ID: <3572CF02.347@biochem.usyd.edu.au> Reply-To: x.wang@biochem.usyd.edu.au NNTP-Posting-Host: bio114.biochem.usyd.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; 68K) Hi, all, Does anyone know the optimal reaction conditions of chymotrypsin digestion?(enzyme/protein ratio, temperature, and time) Thanks. Sue From owner-proteins@net.bio.net Sun May 31 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!csir.uni.net.za!und.uni.net.za!und.ac.za!newsadmin From: Dennison Newsgroups: bionet.molbio.proteins Subject: Re: eluting proteins from gels Date: Mon, 01 Jun 1998 17:09:14 +0000 Organization: University of Natal Lines: 33 Message-ID: <3572E03A.5804@unpsun1.cc.unp.ac.za> References: <357260CB.1EDB15C7@chemi.muni.cz> Reply-To: Biochemistry, Dept NNTP-Posting-Host: bchm4.bchm.unp.ac.za Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-2 Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; 68K) > > Recently, i have been dabbling in a bit of protein purification and on > > a > > coomasie gel i have only a few proteins, one in particular is stronger > > than > > the others. I wish to show that this stronger band is the protein > > responsible for activity I am detecting in the fraction. Is it > > feasible to > > simply elute this band from the SDS gel and assay this for activity, > > i.e. > > is there a simple way of eluting the protein directly from the gel > > with a > > good chance of renaturing the protein? > > > > Thanks in anticipation > > > > Karen Spink > > > > E. mail pdxkgs@pdn1.gene.nottingham.ac.uk We work with proteinases (cathepsins) and we have had no luck in trying to elute activity from polyacrylamide gels. However, gelatin zymograms work well. Gelatin is included in the gel and SDS run in the usual way, except protein is not boiled. After running, the SDS is removed with a triton-X wash and activity is revealed as a clear area upon staining of the gelatin. See Heussen and Dowdle, Anal. Biochem. (1980) 102, 192-202. The point is that the activity is there but for some reason cannot be successfully eluted from the gel. The proteins themselves are successfully eluted, as evidenced by immunoblotting, but they must be screwed up during elution. Cheers Clive From owner-proteins@net.bio.net Sun May 31 23:00:00 1998 Newsgroups: bionet.molbio.proteins Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gip.net!news-raspail.gip.net!news.gsl.net!gip.net!newsfeed.ecrc.net!news.cesnet.cz!rhino.cis.vutbr.cz!news.muni.cz!news From: Vladimir Rotrekl Subject: Re: eluting proteins from gels X-Nntp-Posting-Host: lmfra.sci.muni.cz Content-Type: multipart/mixed; boundary="------------F788E22EB51ABE893AF5212A" To: Karen spink Message-ID: <357260CB.1EDB15C7@chemi.muni.cz> X-Priority: 3 (Normal) Sender: news@news.muni.cz (News Admin) Reply-To: rotrekl@chemi.muni.cz Organization: Laboratoø Molekulární Fyziologie Rostlin References: Mime-Version: 1.0 Date: Mon, 1 Jun 1998 08:05:34 GMT X-Mailer: Mozilla 4.01 [en] (Win95; I) Lines: 62 This is a multi-part message in MIME format. --------------F788E22EB51ABE893AF5212A Content-Type: text/plain; charset=us-ascii Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Content-Transfer-Encoding: 7bit Karen spink wrote: > Recently, i have been dabbling in a bit of protein purification and on > a > coomasie gel i have only a few proteins, one in particular is stronger > than > the others. I wish to show that this stronger band is the protein > responsible for activity I am detecting in the fraction. Is it > feasible to > simply elute this band from the SDS gel and assay this for activity, > i.e. > is there a simple way of eluting the protein directly from the gel > with a > good chance of renaturing the protein? > > Thanks in anticipation > > Karen Spink > > E. mail pdxkgs@pdn1.gene.nottingham.ac.uk I havent heard any method where You can get activity after SDS. Better to try native polyacrylamide gel. Then just cut out the band of interest and check the activity without getting rid of polyacrylamide. But!!! knowing nothing about Your proteine it is hard to predict Your chances. For some enzyme activities there are available activity gel staining procedures (e.g. for gycosidases). good luck Vladimir Rotrekl Dept of biochemistry Masaryk University, Kotlarska 2, 61137 Brno, CZECH REP. --------------F788E22EB51ABE893AF5212A Content-Type: text/x-vcard; charset=us-ascii; name="vcard.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Vladimir Rotrekl Content-Disposition: attachment; filename="vcard.vcf" begin: vcard fn: Vladimir Rotrekl n: Rotrekl;Vladimir org: Laboratory of Plant Molecular Physiology adr: Faculty of Science, Masaryk University;;Kotláøská 2;Brno;;61137;Czech Republic email;internet: rotrekl@chemi.muni.cz title: Mgr. tel;work: (05) 41129374 tel;fax: (05) 41129372 tel;home: (05) 41223629 x-mozilla-cpt: ;0 x-mozilla-html: FALSE end: vcard --------------F788E22EB51ABE893AF5212A-- From owner-proteins@net.bio.net Sun May 31 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!sunqbc.risq.qc.ca!news.uow.edu.au!metro!sunb.ocs.mq.edu.au!not-for-mail From: "sue" Newsgroups: bionet.molbio.proteins Subject: chymotryposin Date: 31 May 1998 03:07:33 GMT Organization: Macquarie University, NSW, Australia Lines: 6 Message-ID: <01bd8c41$86bf78a0$775a6f89@ss-pc1> NNTP-Posting-Host: ssg-pc01.mpce.mq.edu.au X-Newsreader: Microsoft Internet News 4.70.1155 Hi, all, Has anyone used chymotrypsin to digest protein or peptide? Could you please tell me the optimal reaction condition ( ratio, temperature, and reaction time)? Thanks. Sue From owner-proteins@net.bio.net Sun May 31 23:00:00 1998 Path: biosci!bloom-beacon.mit.edu!news.kodak.com!news-nysernet-5.sprintlink.net!news.sprintlink.net!128.122.253.90!newsfeed.nyu.edu!wesley.videotron.net!sunqbc.risq.qc.ca!torn!resunix.sickkids.on.ca!not-for-mail From: Randall C Willis Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc,sci.bio.microbiology,sci.bio.technology Subject: Bioscience Humour is on the Web Date: Mon, 01 Jun 1998 11:12:52 -0400 Organization: The Hospital For Sick Children Lines: 29 Message-ID: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> NNTP-Posting-Host: gandalf.sickkids.on.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.3 IP17) Xref: biosci bionet.molbio.methds-reagnts:67842 bionet.molbio.proteins:12839 bionet.general:30051 Hey there all...just a quick note from your friends at Aliquotes, the journal of bioscience humour and information, to let you know that we have finally joined the 20th Century and have established a Web presence. To those patient readers of our print journal, we thank you and want you to know that this is our new format (except within Canada). To those of you who are new to our magazine, give us a click and let us know what you think. You'll find us at: http://members.tripod.com/~BioHazard5/ just click on the Aliquotes logo in the frame and check out our last few issues. We're hoping to backdate with more issues as they become available. Keep smilin' Randall C Willis, Publisher Aliquotes Press Aliquotes: A Journal Of Molecular and Biochemical Humour and Information 441 Sandlewood Rd. Oakville, ON L6L 3S3 CANADA 905-469-9189 (ph) 416-813-5022 (fax) roger@xybercom.net http://members.tripod.com/~BioHazard5/ From owner-proteins@net.bio.net Sun May 31 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!sunqbc.risq.qc.ca!news.uow.edu.au!metro!sunb.ocs.mq.edu.au!not-for-mail From: "Raina" Newsgroups: bionet.molbio.proteins Subject: chymotrypsin Date: 31 May 1998 11:28:45 GMT Organization: Macquarie University, NSW, Australia Lines: 6 Message-ID: <01bd6b86$8f375600$69f16f89@ibm686.mpce.mq.edu.au> NNTP-Posting-Host: mpce-remote27.mpce.mq.edu.au X-Newsreader: Microsoft Internet News 4.70.1157 Hi, all, Has anyone used chymotrypsin to digest protein or peptide? Could you please tell me the optimal reaction conditions(reaction ratio, temperature,and time)? Thanks. Sue From owner-proteins@net.bio.net Sun May 31 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!logbridge.uoregon.edu!howland.erols.net!news.pagesat.net!news.itis.com!news.doit.wisc.edu!mcmahan From: mcmahan@oncology.wisc.edu (Scott McMahan) Newsgroups: bionet.molbio.proteins Subject: Re: eluting proteins from gels Date: Mon, 01 Jun 1998 19:01:16 +0800 Organization: University of Wisconsin-Madison Lines: 20 Message-ID: References: NNTP-Posting-Host: beta.oncology.wisc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Yet Another NewsWatcher 2.4.0 In article , pdxkgs@pdn1.gene.nott.ac.uk (Karen spink) wrote: :Recently, i have been dabbling in a bit of protein purification and on a :coomasie gel i have only a few proteins, one in particular is stronger than :the others. I wish to show that this stronger band is the protein :responsible for activity I am detecting in the fraction. Is it feasible to :simply elute this band from the SDS gel and assay this for activity, i.e. :is there a simple way of eluting the protein directly from the gel with a :good chance of renaturing the protein? : :Thanks in anticipation : :Karen Spink Try looking at Hager & Burgess (1980) Anal. Biochem. 109, 76-86. -- Scott McMahan mcmahan@oncology.wisc.edu