From owner-proteins@net.bio.net Mon Jun 01 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!chicago-news-feed1.bbnplanet.com!news.bbnplanet.com!newsfeed.enteract.com!news.enteract.com!news.maxwell.syr.edu!newsfeed.nacamar.de!nntp.news.xara.net!xara.net!server5.netnews.ja.net!server3.netnews.ja.net!server1.netnews.ja.net!server2.netnews.ja.net!pegasus.csx.cam.ac.uk!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!gmorley From: gmorley@hgmp.mrc.ac.uk (Mr. G. Morley) Newsgroups: bionet.molbio.proteins Subject: dna strider or similar? Date: 2 Jun 1998 13:19:53 GMT Organization: MRC Human Genome Mapping Project Resource Centre Lines: 9 Message-ID: <6l0u5p$m7a$1@niobium.hgmp.mrc.ac.uk> NNTP-Posting-Host: tin.hgmp.mrc.ac.uk Hi I was wondering if there was a freeware or shareware programme similar to (or better) than DNA strider for analysis of restriction enzyme sites in DNA etc? I know of Webb cutter 2.0 but I would like to down load the programme for my macintosh if possible ... thanks in advance, Gary Morley. gmorley@rpms.ac.uk From owner-proteins@net.bio.net Mon Jun 01 23:00:00 1998 Path: biosci!rutgers!rockyd.rockefeller.edu!newsfeed.nyu.edu!news.idt.net!feed2.news.erols.com!erols!news.mindspring.net!cc.gatech.edu!GT-News!not-for-mail From: nobody@nowhere.com (john doe) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc,sci.bio.microbiology,sci.bio.technology Subject: Re: Bioscience Humour is on the Web Date: Tue, 02 Jun 1998 11:25:41 GMT Organization: dis- Lines: 14 Message-ID: <3573e0ca.2593980@news.gatech.edu> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> NNTP-Posting-Host: ptb350w-1.ipst.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Newsreader: Forte Agent 1.5/32.451 X-No-Archive: yes Xref: biosci bionet.molbio.methds-reagnts:67868 bionet.molbio.proteins:12842 bionet.general:30061 >>[bits deleted] >>You'll find us at: >> >> http://members.tripod.com/~BioHazard5/ >> >> >It's just a pity that it crashes Netscape 3.0 on my system with a javascript error. Works fine >with Internet Explorer 3.0. > >Raymond Dalgleish Must be your system; mine, with Netscape 3.0 has no problems. However, like many sites these days, it may be due to a "site under construction" problem. From owner-proteins@net.bio.net Mon Jun 01 23:00:00 1998 Path: biosci!bloom-beacon.mit.edu!news.kodak.com!news-nysernet-5.sprintlink.net!news.sprintlink.net!128.122.253.90!newsfeed.nyu.edu!feeder.qis.net!news-peer.gip.net!news-lond.gip.net!news.gsl.net!gip.net!nntp.news.xara.net!xara.net!server5.netnews.ja.net!server3.netnews.ja.net!server1.netnews.ja.net!warwick!leicester!pc5 From: ray@le.ac.uk (Raymond Dalgleish) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc,sci.bio.microbiology,sci.bio.technology Subject: Re: Bioscience Humour is on the Web Date: Tue, 02 Jun 98 08:23:35 GMT Organization: Department of Genetics, University of Leicester, UK Lines: 16 Distribution: inet Message-ID: <6l0cq7$ce4_001@gene.le.ac.uk> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> NNTP-Posting-Host: pc5.gene.le.ac.uk X-Newsreader: News Xpress Version 1.0 Beta #4 Xref: biosci bionet.molbio.methds-reagnts:67865 bionet.molbio.proteins:12841 bionet.general:30060 In article <3572C4F4.41C6@gandalf.psf.sickkids.on.ca>, Randall C Willis wrote: >[bits deleted] >You'll find us at: > > http://members.tripod.com/~BioHazard5/ > >just click on the Aliquotes logo in the frame and check out our last few >issues. We're hoping to backdate with more issues as they become >available. > It's just a pity that it crashes Netscape 3.0 on my system with a javascript error. Works fine with Internet Explorer 3.0. Raymond Dalgleish From owner-proteins@net.bio.net Mon Jun 01 23:00:00 1998 Path: biosci!news.Stanford.EDU!newsfeed.concentric.net!newsfeed.direct.ca!newsfeed.nacamar.de!dispose.news.demon.net!demon!delos!server1.netnews.ja.net!pegasus.csx.cam.ac.uk!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!gmorley From: gmorley@hgmp.mrc.ac.uk (Mr. G. Morley) Newsgroups: bionet.molbio.proteins Subject: COS 7 cells Date: 2 Jun 1998 12:02:47 GMT Organization: MRC Human Genome Mapping Project Resource Centre Message-ID: <6l0pl7$kcf$1@niobium.hgmp.mrc.ac.uk> NNTP-Posting-Host: tin.hgmp.mrc.ac.uk Lines: 9 Hello all, a colleague of mine is having difficulty culturing some COS7 cells. The cells are not prolliferating well in media and I was wondering if anyone had any ideas or encountered the same problem. It is unlikely that the foetal calf serum is at fault as the problem has manifested itself quite recently and the fcs batch has not chabged. I would be obligued at any ideas, thanks in advance..... gary morley From owner-proteins@net.bio.net Mon Jun 01 23:00:00 1998 Path: biosci!news.Stanford.EDU!newsfeed.concentric.net!news.he.net!newsfeed.nacamar.de!nntp.news.xara.net!xara.net!baron.netcom.net.uk!netcom.net.uk!server3.netnews.ja.net!news.icnet!mac052061.lif.icnet.uk!user From: i.mcfarlane@icrf.icnet.uk (Ian McFarlane) Newsgroups: bionet.molbio.proteins Subject: Asp-Pro cleavage by H+ Date: Tue, 02 Jun 1998 15:11:12 +0100 Organization: Imperial Cancer Research Fund Lines: 11 Message-ID: NNTP-Posting-Host: mac052061.lif.icnet.uk Hi, Someone recently posted that the NH2-Asp-Pro-COOH bond was labile under acid conditions. Is this true for the NH2-Pro-Asp-COOH pair as well? Also does anyone have a protocol or set of reaction conditions that can be used to cleave the above peptide sequences? Thanks in advance Ian Mc From owner-proteins@net.bio.net Mon Jun 01 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: theodorn@medlib.georgetown.edu Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc Subject: Re: Bioscience Humour is on the Web Date: Tue, 02 Jun 1998 13:42:46 GMT Organization: Deja News - The Leader in Internet Discussion Lines: 30 Message-ID: <6l0vgm$f4a$1@nnrp1.dejanews.com> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> NNTP-Posting-Host: 141.161.229.208 X-Article-Creation-Date: Tue Jun 02 13:42:46 1998 GMT X-Http-User-Agent: Mozilla/4.04 (Macintosh; I; PPC) Xref: biosci bionet.molbio.methds-reagnts:67876 bionet.molbio.proteins:12847 bionet.general:30065 In article <6l0cq7$ce4_001@gene.le.ac.uk>, ray@le.ac.uk (Raymond Dalgleish) wrote: > > In article <3572C4F4.41C6@gandalf.psf.sickkids.on.ca>, > Randall C Willis wrote: > > >[bits deleted] > >You'll find us at: > > > > http://members.tripod.com/~BioHazard5/ > > > >just click on the Aliquotes logo in the frame and check out our last few > >issues. We're hoping to backdate with more issues as they become > >available. > > > It's just a pity that it crashes Netscape 3.0 on my system with a javascript error. Works fine > with Internet Explorer 3.0. > > Raymond Dalgleish > It also crashes Netscape Communicator 4.04 on my system. And this is the most stable version I've had yet. Nick Theodorakis Georgetown University Medical School -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/ Now offering spam-free web-based newsreading From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!rutgers!rockyd.rockefeller.edu!newsfeed.nyu.edu!newsfeed.atl.bellsouth.net!cpk-news-hub1.bbnplanet.com!cam-news-feed2.bbnplanet.com!news.bbnplanet.com!news.indigo.ie!not-for-mail From: Andy Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc,sci.bio.microbiology,sci.bio.technology Subject: Re: Bioscience Humour is on the Web Date: Wed, 03 Jun 1998 12:51:26 +0100 Organization: None Lines: 38 Message-ID: <357538BD.5723DCF3@mindless.com> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <3573e0ca.2593980@news.gatech.edu> NNTP-Posting-Host: ts02-045.dublin.indigo.ie Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.04 [en] (Win95; I) Xref: biosci bionet.molbio.methds-reagnts:67910 bionet.molbio.proteins:12852 bionet.general:30076 The page loads but as soon as the "little gladiator?" starts walking - My system crashes too! (NS4.07) Andy Richard P. Grant wrote: > I wasn't going to say anything, but NS 4 didn't like it either (MacOS8) - > but Norton Crashguard saved the day. > > In article <3573e0ca.2593980@news.gatech.edu>, nobody@nowhere.com (john > doe) wrote: > > : >>[bits deleted] > : >>You'll find us at: > : >> > : >> http://members.tripod.com/~BioHazard5/ > : >> > : > : >> > : >It's just a pity that it crashes Netscape 3.0 on my system with a > javascript error. Works fine > : >with Internet Explorer 3.0. > : > > : >Raymond Dalgleish > : Must be your system; mine, with Netscape 3.0 has no problems. However, > : like many sites these days, it may be due to a "site under > : construction" problem. > > -- > Richard P. Grant MA DPhil | rgrant at cmtech.co.uk > Senior R&D Scientist | work: http://www.cmtech.co.uk/ > Cambridge Molecular | home: http://www.avnet.co.uk/adastra > -- Standard corporate disclaimers apply -- From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gip.net!news-raspail.gip.net!news.gsl.net!gip.net!newscore.univie.ac.at!193.171.255.24.MISMATCH!newsfeed03.univie.ac.at!03-newsfeed.univie.ac.at!news.univie.ac.at!not-for-mail From: a8803349.nospam@unet.univie.ac.at (Martin Offterdinger) Newsgroups: bionet.molbio.proteins Subject: Protein sequencing questions Date: Wed, 03 Jun 1998 08:07:38 GMT Organization: AKH Lines: 30 Message-ID: <3575043c.573691@news.univie.ac.at> NNTP-Posting-Host: grunt.imed1.akh-wien.ac.at X-Newsreader: Forte Free Agent 1.1/16.230 Hello everyone! We have isolated a 150kD protein from cell nuclei; we think we know the identity of the protein as we have used specific antibodies(3 different ones) to IP the protein from nuclear extracts. As this protein should not be localised in nuclei normally, we want to confirm its identity by sequencing. We have been told that very often proteins of mammalian origin have modifications at the N-terminus that prevent direct sequencing via edman degradation. We were suggested to chemicallly or enzymatically cleave the protein and have the fragments sequenced. As we have no experience in this i would like to ask the following questions. Is chemical cleavage more desireable than protease digestion if you expect a certain sequence? What is the more reliable method: CNbr or hydroxylamine cleavage ? (we would prefer the latter as this would leave us with less fragments) Is there anyone who has good protocols for "on blot digestion/cleavage" Are there companies out there prefeably in Europe who would do this for us?? I am lookin forward to discussion! Martin Martin Offterdinger Internal Med.I,Dept. Oncology University of Vienna Austria E-Mail:a8803349.nospam@unet.univie.ac.at (remove nospam before mailing) From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!uninett.no!newscore.univie.ac.at!newsmaster-01.vbs.at!newsfeed03.univie.ac.at!03-newsfeed.univie.ac.at!news.univie.ac.at!not-for-mail From: a8803349.nospam@unet.univie.ac.at (Martin Offterdinger) Newsgroups: bionet.molbio.proteins Subject: fluorescence based protein assay Date: Wed, 03 Jun 1998 08:05:19 GMT Organization: AKH Lines: 15 Message-ID: <34dad227.4584040@news.univie.ac.at> NNTP-Posting-Host: grunt.imed1.akh-wien.ac.at X-Newsreader: Forte Free Agent 1.1/16.230 Hi! I wonder if anyone has tried fluorescence based protein assays like nano orange from molecular probes? I would like to use it because I have a small amount(5-7ul) of a dilute sample. Is there any colorimetric assay which can achieve the same sensitivity (10ng protein/ml)? What are your experiences with fluorescent protein assays? Currently I m using Bradford, but this is clearly not enough sensitive! martin Martin Offterdinger Internal Med.I,Dept. Oncology University of Vienna Austria E-Mail:a8803349.nospam@unet.univie.ac.at (remove nospam before mailing) From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!rill.news.pipex.net!pipex!server1.netnews.ja.net!pegasus.csx.cam.ac.uk!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!gmorley From: gmorley@hgmp.mrc.ac.uk (Mr. G. Morley) Newsgroups: bionet.molbio.proteins Subject: cell line database? Date: 3 Jun 1998 08:29:00 GMT Organization: MRC Human Genome Mapping Project Resource Centre Lines: 8 Message-ID: <6l31gc$nhd$1@niobium.hgmp.mrc.ac.uk> NNTP-Posting-Host: tin.hgmp.mrc.ac.uk Dear all, I was wondering whether anyone knows of a database of the different cell lines that are floating around? Preferably giving phenotypes etc. Mainly I was wondering about 32D haemopoeitic cells in particular. I would appreciate any web addresses etc. thanks in advance, gary morley gmorley@rpms.ac.uk From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!rutgers!rockyd.rockefeller.edu!newsfeed.nyu.edu!news.idt.net!feed2.news.erols.com!erols!howland.erols.net!torn!resunix.sickkids.on.ca!not-for-mail From: Randall C Willis Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc,sci.bio.microbiology,sci.bio.technology Subject: Aliquotes Crashes (WAS: Bioscience Humour is on the Web) Date: Wed, 03 Jun 1998 09:27:49 -0400 Organization: The Hospital For Sick Children Lines: 20 Message-ID: <35754F55.41C6@gandalf.psf.sickkids.on.ca> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <3573e0ca.2593980@news.gatech.edu> <357538BD.5723DCF3@mindless.com> NNTP-Posting-Host: gandalf.sickkids.on.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.3 IP17) Xref: biosci bionet.molbio.methds-reagnts:67914 bionet.molbio.proteins:12853 bionet.general:30077 Thank you to everyone who posted regarding their computer problems when trying to click onto our website...I will talk to our technical people and see if they can determine the problem. Are there others out there who are not having a problem and with what browser and software? Randy W Randall C Willis, Publisher Aliquotes Press Aliquotes: A Journal Of Molecular and Biochemical Humour and Information 441 Sandlewood Rd. Oakville, ON L6L 3S3 CANADA 905-469-9189 (ph) 416-813-5022 (fax) roger@xybercom.net http://members.tripod.com/~BioHazard5/ From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!agate!logbridge.uoregon.edu!news.maxwell.syr.edu!news.cis.ohio-state.edu!magnus.acs.ohio-state.edu!not-for-mail From: " Arnold N. DuBell" Newsgroups: bionet.molbio.proteins Subject: Source for tyrphostin AG18? Date: Wed, 03 Jun 1998 10:30:30 -0400 Organization: The Ohio State University College of Medicine Lines: 11 Message-ID: <35755E06.6C3BCC55@osu.edu> NNTP-Posting-Host: pharm18.med.ohio-state.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.03 [en] (Win95; I) Hi- Does anyone know of a supplier for the above tyrosine kinase inhibitor? Thank you in advance for any replies, Arnold DuBell (email: dubell.1@osu.edu) From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!boston-news-feed1.bbnplanet.com!news.bbnplanet.com!nutmeg.uchc.edu!not-for-mail From: "Heinz J. Schaefers" Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc Subject: Re: Bioscience Humour is on the Web Date: Wed, 03 Jun 1998 11:03:26 -0400 Organization: Univ of CT Health Center Lines: 39 Message-ID: <357565BE.548F33E4@sun.XuchcX.edu> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <6l0vgm$f4a$1@nnrp1.dejanews.com> NNTP-Posting-Host: 155.37.5.239 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.04 [en] (WinNT; I) Xref: biosci bionet.molbio.methds-reagnts:67921 bionet.molbio.proteins:12855 bionet.general:30078 theodorn@medlib.georgetown.edu wrote: > In article <6l0cq7$ce4_001@gene.le.ac.uk>, > ray@le.ac.uk (Raymond Dalgleish) wrote: > > > > In article <3572C4F4.41C6@gandalf.psf.sickkids.on.ca>, > > Randall C Willis wrote: > > > > >[bits deleted] > > >You'll find us at: > > > > > > http://members.tripod.com/~BioHazard5/ > > > > > >just click on the Aliquotes logo in the frame and check out our last few > > >issues. We're hoping to backdate with more issues as they become > > >available. > > > > > It's just a pity that it crashes Netscape 3.0 on my system with a javascript error. Works fine > > with Internet Explorer 3.0. > > > > Raymond Dalgleish > > > > It also crashes Netscape Communicator 4.04 on my system. And this is the most > stable version I've had yet. > > Nick Theodorakis > Georgetown University Medical School > > -----== Posted via Deja News, The Leader in Internet Discussion ==----- > http://www.dejanews.com/ Now offering spam-free web-based newsreading well, here it works fine... Netscape Communicator Pro 4.05 on a P166 using NT4 server with SP3 Heinz From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!sunqbc.risq.qc.ca!news.uow.edu.au!metro!munnari.OZ.AU!bunyip.cc.uq.edu.au!not-for-mail From: JAnderson@botany.uq.edu.au (Jonathan Anderson) Newsgroups: bionet.molbio.proteins Subject: NaCl and Cyanogen Bromide Date: 3 Jun 1998 09:17:30 GMT Organization: Dept of Botany, Univ. of Queensland Lines: 7 Message-ID: <6l34ba$srl$1@bunyip.cc.uq.edu.au> NNTP-Posting-Host: 130.102.6.165 Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.8 (16bit) Hi all, Does anyone know if NaCl concentration effects CNBr cleavage? Thanks. Jon. From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!nntprelay.mathworks.com!gatech!nntp-xfer.ncsu.edu!calvert From: calvert@eos.ncsu.edu (Phil Calvert) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,sci.chem.analytical Subject: Stable Amino Acid Derivative for HPLC? Date: Wed, 03 Jun 1998 17:19:05 -0400 Organization: North Carolina State University Lines: 22 Message-ID: NNTP-Posting-Host: mac-27rd.ce.ncsu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-no-archive: no X-Newsreader: Yet Another NewsWatcher 2.4.0 Xref: biosci bionet.molbio.methds-reagnts:67936 bionet.molbio.proteins:12861 There a number of methods available for derivatizing amino acids and analyzing them by reverse-phase HPLC on a C18 column. Unfortunately, many of these derivatives are not very stable. Obviously, they are stable enough for analytical purposes. But I am interested in doing preparative HPLC and isolating the purified amino acid derivatives; so if the derivatives aren't very stable, that sort of defeats the purpose. I would like to use a UV detector for detecting the derivatized amino acids. Therefore, derivatives that are UV-absorbing (including fluorescent derivatives) are preferred (provided that they are stable). Anyone know of any derivatives that fit these qualifications? Phil Calvert -- ------------------------------------ !-----------Phil Calvert-----------! !---calvert.NoSpam@eos.ncsu.edu----! ------------------------------------ NOTE: If present, the phrase ".NoSpam" must be removed from my return address before sending your reply. From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!newsfeed.concentric.net!news-peer-west.sprintlink.net!news-peer.sprintlink.net!news.sprintlink.net!newsfeed.nacamar.de!fu-berlin.de!news.ruhr-uni-bochum.de!not-for-mail From: "Thorsten Schmidt" Newsgroups: bionet.molbio.proteins Subject: How to mount "Cy 2"? Date: 3 Jun 1998 19:46:46 GMT Organization: Ruhr-Universitaet Bochum, Rechenzentrum Lines: 25 Message-ID: <01bd8f28$5592f4c0$e3019386@schmidtdw.rz.ruhr-uni-bochum.de> NNTP-Posting-Host: dialppp-1-227.rz.ruhr-uni-bochum.de X-Newsreader: Microsoft Internet News 4.70.1155 Dear reader! I would like to mount cells which were incubated with a Cy2-conjugated secondary antibody in an immunfluorescence experiment. For FITC I used Vectashield (Vector) and made best experience. Without Vectashield (in just PBS-Glycerol) the fluorescence fades away in seconds. But Vectashield prolonged the stability of the dye to hours. I heard that it is tricky to mount Cy2 because some fluorecence-stabilizer might react with the Cyanine-dye and destroy it and that one must not use Vectashield. So: Which other mounting medium can I use to mount Cy2? (and where can I get it?) Is it possible to add any fluorecence stabilizers? Or is it not necessary to stabilize Cy2 because of the long (advertised) photostability? Thank you so much in advance for an answer. Thorsten Schmidt From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!newsfeed.sgi.net!pitt.edu!not-for-mail From: pxpst2@SPAM.SUXS.unixs.cis.pitt.edu (Peter) Newsgroups: bionet.molbio.proteins Subject: Re: Protein sequencing questions Date: Wed, 03 Jun 1998 21:19:32 -0500 Organization: University of Pittsburgh Lines: 64 Message-ID: References: <3575043c.573691@news.univie.ac.at> NNTP-Posting-Host: pelli.pathology.pitt.edu Mail-Copies-To: pxpst2@SPAM.SUXS.unixs.cis.pitt.edu X-Newsreader: MT-NewsWatcher 2.4.4 In article <3575043c.573691@news.univie.ac.at>, a8803349.nospam@unet.univie.ac.at (Martin Offterdinger) wrote: > Hello everyone! > We have isolated a 150kD protein from cell nuclei; we think we know > the identity of the protein as we have used specific antibodies(3 > different ones) to IP the protein from nuclear extracts. As this > protein should not be localised in nuclei normally, we want to confirm > its identity by sequencing. We have been told that very often proteins > of mammalian origin have modifications at the N-terminus that prevent > direct sequencing via edman degradation. We were suggested to > chemicallly or enzymatically cleave the protein and have the fragments > sequenced. As we have no experience in this i would like to ask the > following questions. N terminal blocking is most common in serum proteins but bad handling can block the end as well. Anyway, unless you are going to do the sequencing it is advised that you do not do this yourself. Whomever you find to do the sequencing will digest the protein with specialty enzymes( modified trypsin, V8, etc...). Since your protein is so large, coventional techniques of sequencing via HPLC will be difficult to say the least because those techniques require about 10-50uM. That is a lot of protein. A really "good" lab could maybe do 10-100 fold lower than that but it is difficult and still a lot of protein. The type of lab you should look for is a Mass Spectrocopy sequencing lab, they can sequence at best femtomolar quantities at worst picamolar concentrations. > > Is chemical cleavage more desireable than protease digestion if you > expect a certain sequence? Enzymes used are not the Wild type strains. Either will work. > What is the more reliable method: CNbr or hydroxylamine cleavage ? > (we would prefer the latter as this would leave us with less > fragments) A large fragment is harder to sequence completely and in my opinion you only need a partial sequence so does it matter that you have just a few fragments or a lot. All fragments will have to be purified before sequencing anyway. > Is there anyone who has good protocols for "on blot > digestion/cleavage" Das geht nicht. You can not digest well at all on a membrane. > Are there companies out there prefeably in Europe who would do this > for us?? Companies are not this far along but there are Academic labs in Germany that pioneered mass spec sequencing. I will look them up and send you their name > I am lookin forward to discussion! > Martin Peter Pediaditakis -- "Don't you eat that yellow snow watch out where the Huskies go" FZ --------------------------------------------------------------------- From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!newsfeed.concentric.net!news-peer-west.sprintlink.net!news-peer.sprintlink.net!news.sprintlink.net!news-peer.gip.net!news-dc.gip.net!news.gsl.net!gip.net!duke.telepac.pt!news.telepac.pt!brown.telepac.pt!news.telepac.pt!news From: "Helder M. Vieira" Newsgroups: bionet.molbio.proteins Subject: Differentiation signals Date: Thu, 4 Jun 1998 00:23:37 +0100 Lines: 35 Message-ID: <6l4p5n$fmk@brown.telepac.pt> NNTP-Posting-Host: poras4-p45.telepac.pt X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 Hi ! I'm not a biologist, just a programmer in the LIS area. My interest in molecular biology has been growing steadily in the recent past, but I'm currently blocked in a dead-end. Simply put, the problem is this: 1. If I think about an embryo with around 1000 cells, with some kind of differentiation already going on, it's not hard to accept the idea of the cascade of differentiation signals that will lead to the final configuration of the colony (one head, at least two arms, etc., etc...) 2. For smaller populations, I start to think about the minimal signaling needed to start the process. And, for a mammal, I'd accept if someone said that the only information needed is the definition of the head/tail axis. After formation of the neural tube, bilateral simmetry doesn't seem a tremendous problem. Correct me if I'm wrong. 3. My real problem is what starts the first differentiation, leading to the formation of the neural tube. I can't find this information anywhere. I'd say that, in mammals, the egg is homogeneous and therefore unable to establish some kind of gradient. So, the first signal would have to come from the outside... This probably IS a stupid problem, but could someone please give me an answer ? Thanks Helder M. Vieira From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!agate!nature.berkeley.edu!lhom From: lhom@nature.berkeley.edu (Louis Hom) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc Subject: Re: Bioscience Humour is on the Web Date: 3 Jun 1998 23:29:57 GMT Organization: University of California, Berkeley Lines: 12 Message-ID: <6l4m9l$amc$1@agate.berkeley.edu> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <6l0vgm$f4a$1@nnrp1.dejanews.com> <3575c736.1319130@news-bas-st-laurent.globetrotter.net> NNTP-Posting-Host: nature.berkeley.edu Xref: biosci bionet.molbio.methds-reagnts:67942 bionet.molbio.proteins:12863 bionet.general:30089 In article <3575c736.1319130@news-bas-st-laurent.globetrotter.net>, Michael Black, Ph.D. wrote: > >Any chance it's a problem with the "Welcome to Tripod..." pop-up? The It loaded fine in Cyberdog and NN 4.x. NN 2.x said there were JavaScript errors in lines 18 and 19. -- _______________________________________________________________________________ Lou Hom >K '93 lhom@nature.berkeley.edu http://www.ocf.berkeley.edu/~lhom From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!sunqbc.risq.qc.ca!news.quebectel.com!not-for-mail From: mbblack@globetrotter.qc.ca (Michael Black, Ph.D.) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc Subject: Re: Bioscience Humour is on the Web Date: Wed, 03 Jun 1998 21:59:43 GMT Organization: GlobeTrotter Lines: 32 Message-ID: <3575c736.1319130@news-bas-st-laurent.globetrotter.net> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <6l0vgm$f4a$1@nnrp1.dejanews.com> NNTP-Posting-Host: ts1-32.f104.quebectel.com X-Newsreader: Forte Free Agent 1.11/32.235 Xref: biosci bionet.molbio.methds-reagnts:67939 bionet.molbio.proteins:12862 bionet.general:30088 On Tue, 02 Jun 1998 13:42:46 GMT, theodorn@medlib.georgetown.edu wrote: > >It also crashes Netscape Communicator 4.04 on my system. And this is the most >stable version I've had yet. > >Nick Theodorakis >Georgetown University Medical School > > >-----== Posted via Deja News, The Leader in Internet Discussion ==----- >http://www.dejanews.com/ Now offering spam-free web-based newsreading Any chance it's a problem with the "Welcome to Tripod..." pop-up? The site works fine under IE 3.02 and Netscape 4.02 on both my work (486Sx/50) and home machines (Pentium laptop). However, on a friends Pentium desktop at work, whenever we close the pop-up, SNAP, we get a browser error (working with Netscape 3.something). It's similar to crashes I've had on GeoCities sites I've seen since they started having a pop-up on their members sites. Just wondering... Cheers, Michael -- Michael Black, Ph.D. Post-Doctoral Fellow Fisheries & Oceans, Canada mbblack@globetrotter.qc.ca * opinions expressed are, of course, * * merely my own personal ones * From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!howland.erols.net!news-peer.gip.net!news-lond.gip.net!news.gsl.net!gip.net!dispose.news.demon.net!demon!peer.news.zetnet.net!peer.news.bb.u-net.net!u-net!yama.mcc.ac.uk!hydraulix.bangor.ac.uk!manager From: Keith James Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc,sci.bio.microbiology,sci.bio.technology Subject: Re: Aliquotes Crashes (WAS: Bioscience Humour is on the Web) Followup-To: bionet.general Date: 03 Jun 1998 18:07:45 +0100 Organization: University of Wales, Bangor. Sender: bss194@pwilliams.bangor.ac.uk Distribution: inet Message-ID: References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <3573e0ca.2593980@news.gatech.edu> <357538BD.5723DCF3@mindless.com> <35754F55.41C6@gandalf.psf.sickkids.on.ca> NNTP-Posting-Host: pwilliams.bangor.ac.uk X-Newsreader: Gnus v5.3/Emacs 19.34 Lines: 17 Xref: biosci bionet.molbio.methds-reagnts:67932 bionet.molbio.proteins:12857 bionet.general:30084 >>>>> "Randall" == Randall C Willis writes: Randall> Are there others out there who are not having a problem Randall> and with what browser and software? No problem here. I don't usually allow Java(script) to run, so I switched it on and reloaded your page. Still no problems. Running: Linux kernel 2.0.27 (i386) Netscape Communicator 4.04 (Red Hat rpm distribution) Junkbuster proxy (Junkbuster-blank-2.0-1) -- Keith James -- k.james@bangor.ac.uk -- http://www.bangor.ac.uk/~bss194 Biodegradation Group - School of Biological Sciences - University of Wales PGP preferred when your e-mail is not for 3rd parties - www for Public Key Public Key fingerprint is 8F 60 5A 4D DB A9 23 65 7D 95 62 19 F0 9C A8 E0 From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Date: Wed, 03 Jun 1998 17:06:20 +0100 From: roney.graf@uni-konstanz.de (Roney Graf) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc,sci.bio.microbiology,sci.bio.technology Subject: Re: Bioscience Humour is on the Web Message-ID: References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <3573e0ca.2593980@news.gatech.edu> <357538BD.5723DCF3@mindless.com> Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-transfer-encoding: 8bit X-Newsreader: Yet Another NewsWatcher 2.4.0 NNTP-Posting-Host: hermann.biologie.uni-konstanz.de Organization: University of Constance, Germany Lines: 11 Path: biosci!bloom-beacon.mit.edu!news.kodak.com!news-nysernet-5.sprintlink.net!news.sprintlink.net!128.122.253.90!newsfeed.nyu.edu!nntprelay.mathworks.com!fu-berlin.de!news.uni-stuttgart.de!news.belwue.de!news.uni-konstanz.de!roney.graf Xref: biosci bionet.molbio.methds-reagnts:67922 bionet.molbio.proteins:12856 bionet.general:30079 In article <357538BD.5723DCF3@mindless.com>, Andy wrote: > The page loads but as soon as the "little gladiator?" starts walking - > My system crashes too! (NS4.07) Net$cape 2.02. Apple Mac LC, 10MB, System 7.1. Not exactly the most advanced of configurations. No problems. rg From owner-proteins@net.bio.net Tue Jun 02 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!164.67.42.145!awabi.library.ucla.edu!137.82.194.1!unixg.ubc.ca!hoekstra From: hoekstra@unixg.ubc.ca (Ken Hoekstra) Newsgroups: bionet.molbio.proteins,bionet.neuroscience Subject: "Measuring" aging in tissues Date: 3 Jun 1998 18:54:58 GMT Organization: University of British Columbia, Vancouver, B.C., Canada Lines: 14 Message-ID: <6l4662$hbf$1@nntp.ucs.ubc.ca> NNTP-Posting-Host: netinfo2.ubc.ca X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.molbio.proteins:12859 bionet.neuroscience:23277 I'm looking for some helpful advice from scientists who study aging in animal models. I would like some simple parameter to measure different age groups in my animal model experiments. What are some good parameters, yet not overly time consuming, to measure aging in animal tissues? Are measures in protein oxidation the best? (protein carbonyl content, etc.) Or are there simpler, more rapid measurements used? Any help or direction is appreciated. Thank-you Ken From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!news.mel.connect.com.au!harbinger.cc.monash.edu.au!towncrier.cc.monash.edu.au!not-for-mail From: Mark Cauchi Newsgroups: bionet.molbio.proteins Subject: Recombinant DNA Techniques Course Date: Thu, 04 Jun 1998 17:04:56 +1100 Organization: Monash University Lines: 8 Message-ID: <35763908.28AD@med.monash.edu.au> Reply-To: Mark.Cauchi@med.monash.edu.au NNTP-Posting-Host: microb36.med.monash.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win16; I) The Micromon Unit at the Department of Microbiology, Monash University in Melbourne, Australia will be running its Recombinant DNA Techniques Course between the 15-20 November, 1998. This is an introductory-intermediate level course which offers a skills-based training package. If you would like further information, details can be found on our web page at http://www.med.monash.edu.au/micro/department/dnacorse.htm From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!pravda.ucr.edu!awabi.library.ucla.edu!208.134.241.18!newsfeed.internetmci.com!194.72.7.126!btnet-peer!btnet!nntp.news.xara.net!xara.net!server5.netnews.ja.net!server3.netnews.ja.net!server4.netnews.ja.net!news.cc.ic.ac.uk!not-for-mail From: Koen De Smet Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc,sci.bio.microbiology,sci.bio.technology Subject: Re: Bioscience Humour is on the Web Date: Thu, 04 Jun 1998 08:00:22 +0100 Organization: Department of Medical Microbiology Lines: 29 Message-ID: <35764606.BC6@nospam.ic.ac.uk> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <3573e0ca.2593980@news.gatech.edu> <357538BD.5723DCF3@mindless.com> Reply-To: k.desmet@nospam.ic.ac.uk NNTP-Posting-Host: cb-22.sm.med.ic.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Xref: biosci bionet.molbio.methds-reagnts:67952 bionet.molbio.proteins:12866 bionet.general:30094 Roney Graf wrote: > > In article <357538BD.5723DCF3@mindless.com>, Andy > wrote: > > > The page loads but as soon as the "little gladiator?" starts walking - > > My system crashes too! (NS4.07) > > Net$cape 2.02. Apple Mac LC, 10MB, System 7.1. Not exactly the most > advanced of configurations. No problems. > > rg Netscape 2.02. Apple Mac 6100/60, 16MB, System 7.5. Should be more advanced than the above, but it crashed on accessing this page. Mind you, it often crashes while accessing certain sites, so now I have a list of pages that have a high likelyhood of crashing (including home.netscape!) -- Koen De Smet ============================================================== ==>> To reply by email, remove "nospam." from the address <<== Imperial College School of Medicine at St Mary's http://www.sm.ic.ac.uk/medmicro/home. ============================================================== From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gip.net!news-dc.gip.net!news.gsl.net!gip.net!duke.telepac.pt!news.telepac.pt!brown.telepac.pt!news.telepac.pt!news From: "Helder M. Vieira" Newsgroups: bionet.molbio.proteins Subject: Differentiation signals Date: Thu, 4 Jun 1998 00:23:37 +0100 Lines: 35 Message-ID: <6l5nl7$nrr@brown.telepac.pt> NNTP-Posting-Host: poras8-p37.telepac.pt X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 Hi ! I'm not a biologist, just a programmer in the LIS area. My interest in molecular biology has been growing steadily in the recent past, but I'm currently blocked in a dead-end. Simply put, the problem is this: 1. If I think about an embryo with around 1000 cells, with some kind of differentiation already going on, it's not hard to accept the idea of the cascade of differentiation signals that will lead to the final configuration of the colony (one head, at least two arms, etc., etc...) 2. For smaller populations, I start to think about the minimal signaling needed to start the process. And, for a mammal, I'd accept if someone said that the only information needed is the definition of the head/tail axis. After formation of the neural tube, bilateral simmetry doesn't seem a tremendous problem. Correct me if I'm wrong. 3. My real problem is what starts the first differentiation, leading to the formation of the neural tube. I can't find this information anywhere. I'd say that, in mammals, the egg is homogeneous and therefore unable to establish some kind of gradient. So, the first signal would have to come from the outside... This probably IS a stupid problem, but could someone please give me an answer ? Thanks Helder M. Vieira From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!pravda.ucr.edu!awabi.library.ucla.edu!208.134.241.18!newsfeed.internetmci.com!205.252.116.205!howland.erols.net!frankfurt.de.uu.net!news.uni-stuttgart.de!news.urz.uni-heidelberg.de!usenet From: un691cs@genius.embnet.dkfz-heidelberg.de (Clemens Suter-Crazzolara, PhD) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc Subject: Re: Bioscience Humour is on the Web Date: 4 Jun 1998 07:29:23 GMT Organization: University of Heidelberg, Germany Lines: 21 Message-ID: <6l5icj$aiv@sun0.urz.uni-heidelberg.de> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <6l0vgm$f4a$1@nnrp1.dejanews.com> <3575c736.1319130@news-bas-st-laurent.globetrotter.net> <6l4m9l$amc$1@agate.berkeley.edu> NNTP-Posting-Host: 129.206.158.67 Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: WinVN 0.99.8 (16bit) Xref: biosci bionet.molbio.methds-reagnts:67957 bionet.molbio.proteins:12868 bionet.general:30095 On 3 Jun 1998 23:29:57 GMT, Louis Hom said: >In article <3575c736.1319130@news-bas-st-laurent.globetrotter.net>, >Michael Black, Ph.D. wrote: >>Any chance it's a problem with the "Welcome to Tripod..." pop-up? The > It loaded fine in Cyberdog and NN 4.x. NN 2.x said there were >JavaScript errors in lines 18 and 19. Well, this tread "Re: Bioscience Humour is on the Web" is certainly (and most likely unintentionally) turning into something funny. clemens From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!agate!logbridge.uoregon.edu!news-peer.gip.net!news-stkh.gip.net!news.gsl.net!gip.net!newsfeed1.funet.fi!ousrvr3.oulu.fi!sun1.oulu.fi!pkursula From: Pete Newsgroups: bionet.molbio.proteins Subject: small proteins query Date: Thu, 4 Jun 1998 16:22:40 +0300 Organization: University of Oulu Lines: 12 Message-ID: NNTP-Posting-Host: sun1.oulu.fi Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: pkursula@sun1.oulu.fi Anyone out there with speciality in small proteins? I detect an unknown protein with a mw between 5 and 10 kDa. What could it be? It's a cytoplasmic protein, probably associated with membranous structures, however. It only extracts from tissue homogenates with detergent. Pete From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.direct.ca!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: silvis_r@hotmail.com Newsgroups: bionet.molbio.proteins Subject: HELP ME!? IgY extraction What goes wrong? Date: Thu, 04 Jun 1998 12:37:18 GMT Organization: Deja News - The Leader in Internet Discussion Lines: 17 Message-ID: <6l64du$s6b$1@nnrp1.dejanews.com> NNTP-Posting-Host: 137.120.21.20 X-Article-Creation-Date: Thu Jun 04 12:37:18 1998 GMT X-Http-User-Agent: Mozilla/2.0 (compatible; MSIE 3.01; Windows 95) Hello, i need some help with IgY extraction from egg yolk. I got a tip from someone to put the egg yolk overnight at 4 degr Centigrade in Phosphate bufferd salt (PBS). the following morning i added 3,5% of PEG 6000 to remove any unwanted lipoproteens and other proteens. After centifugion at 5000 G at 30 min i got the wanted sediment. I than added 12% PEG 6000 to the supernatant and swong it around at 5000 G for 30 min at first, i got no result so i have put it back at 12000 G 30 min., still i have no result. What went wrong, why doesn't my IgY form a sediment, i have a turbid suspension!? Tanx for your help -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/ Now offering spam-free web-based newsreading From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!news.pagesat.net!news.itis.com!news.doit.wisc.edu!mcmahan From: mcmahan@oncology.wisc.edu (Scott McMahan) Newsgroups: bionet.molbio.proteins Subject: Re: Asp-Pro cleavage by H+ Date: Fri, 05 Jun 1998 00:40:12 -0600 Organization: University of Wisconsin-Madison Lines: 29 Message-ID: References: NNTP-Posting-Host: alpha.oncology.wisc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Yet Another NewsWatcher 2.4.0 In article , i.mcfarlane@icrf.icnet.uk (Ian McFarlane) wrote: :Hi, Hi, nice to meet you. :Someone recently posted that the NH2-Asp-Pro-COOH bond was labile under :acid conditions. Is this true for the NH2-Pro-Asp-COOH pair as well? Hydrolysis of all amide bonds is acid catalyzed, but the D-P is more susceptible than any other, including P-D. : Also :does anyone have a protocol or set of reaction conditions that can be used :to cleave the above peptide sequences? D-P cleavage conditions can be found in Meth. Enzymol. 47, 145-9. AFAIK, there is no specific method for cleaving P-D bonds. There is an endoprotease that cleaves c-terminal to all prolines, but that requires that the target protein be small (<10 kDa IIRC). :Thanks in advance You're welcome. -- Scott McMahan mcmahan@oncology.wisc.edu From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!news.Stanford.EDU!newsfeed.concentric.net!newsfeed1.earthlink.net!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!not-for-mail From: sshiu@students.wisc.edu (Shinhan Shiu) Newsgroups: bionet.molbio.proteins Subject: Protease inhibitor cocktail Date: Thu, 4 Jun 1998 20:09:23 -0500 Organization: University of Wisconsin, Madison Lines: 11 Message-ID: NNTP-Posting-Host: t-cssc-5-2-p9.dialup.wisc.edu X-Newsreader: Anawave Gravity v2.00 Dear netter, I routinely isolate membrane protein from Arabidopsis. According to the protocol I have, all the protease inhibitors are added individually including pepstatin in MeOH, leupeptin in H2O, aprotinin in H2O, and PMSF in isopropanol. My question is: are you aware of a recipe where you mix everything up like a "protease cocktail"? Thanks in advance. From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-feed.inet.tele.dk!bofh.vszbr.cz!masternews.telia.net!News.Amsterdam.UnisourceCS!news.wxs.nl!not-for-mail From: Marco van de Weert Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,sci.chem.analytical Subject: Re: Stable Amino Acid Derivative for HPLC? Date: Thu, 04 Jun 1998 20:22:11 +0200 Organization: World Access Lines: 22 Message-ID: <3576E5D3.2290@wxs.nl> References: Reply-To: aweert@wxs.nl NNTP-Posting-Host: amf1325.wxs.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01C-WXS-16 (Win95; I) To: Phil Calvert Xref: biosci bionet.molbio.methds-reagnts:67981 bionet.molbio.proteins:12876 Phil Calvert wrote: > > There a number of methods available for derivatizing amino acids and > analyzing them by reverse-phase HPLC on a C18 column. Unfortunately, many > of these derivatives are not very stable. Obviously, they are stable > enough for analytical purposes. But I am interested in doing preparative > HPLC and isolating the purified amino acid derivatives; so if the > derivatives aren't very stable, that sort of defeats the purpose. > > I would like to use a UV detector for detecting the derivatized amino > acids. Therefore, derivatives that are UV-absorbing (including fluorescent > derivatives) are preferred (provided that they are stable). Anyone know of > any derivatives that fit these qualifications? > > Phil Calvert AFAI remember, derivatizing them with ortho-phtalaldehyde (the OPA reagent, or fluoraldehyde) is reasonably stable Marco From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!nntprelay.mathworks.com!gatech!nntp-xfer.ncsu.edu!calvert From: calvert@eos.ncsu.edu (Phil Calvert) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,sci.chem.analytical Subject: Re: Stable Amino Acid Derivative for HPLC? Date: Thu, 04 Jun 1998 14:19:45 -0400 Organization: North Carolina State University Lines: 25 Distribution: world Message-ID: References: NNTP-Posting-Host: mac-27rd.ce.ncsu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-no-archive: no X-Newsreader: Yet Another NewsWatcher 2.4.0 Xref: biosci bionet.molbio.methds-reagnts:67980 bionet.molbio.proteins:12875 In article , David Reeve wrote: > Aren't dansyl derivatives reasonably stable? I seem to remember seeing > purified dansyl-amino acids in the Sigma catalogue. Dave, That's a good point. As I was looking over different possibilities last night, I decided to look up the cost of dansyl-Cl in the Sigma catalog. On the same page were a bunch of dansyl derivatives of various amines and amino acids. So it would seem that they are reasonably stable, at least when dry. And the derivatization is very simple, which is a plus. I'd say that this derivative seems like a good candidate. Phil -- ------------------------------------ !-----------Phil Calvert-----------! !---calvert.NoSpam@eos.ncsu.edu----! ------------------------------------ NOTE: If present, the phrase ".NoSpam" must be removed from my return address before sending your reply. From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!baron.netcom.net.uk!netcom.net.uk!nntp.news.xara.net!xara.net!server5.netnews.ja.net!server3.netnews.ja.net!server4.netnews.ja.net!server2.netnews.ja.net!pegasus.csx.cam.ac.uk!lyra.csx.cam.ac.uk!hgmp.mrc.ac.uk!gmorley From: gmorley@hgmp.mrc.ac.uk (Mr. G. Morley) Newsgroups: bionet.molbio.proteins Subject: re: differentiation signals. Date: 4 Jun 1998 10:40:49 GMT Organization: MRC Human Genome Mapping Project Resource Centre Lines: 20 Message-ID: <6l5tjh$49u$1@niobium.hgmp.mrc.ac.uk> NNTP-Posting-Host: tin.hgmp.mrc.ac.uk >3. My real problem is what starts the first differentiation, leading to the >formation of the neural tube. I can't find this information anywhere. I'd >say that, in mammals, the egg is homogeneous and therefore unable to >establish some kind of gradient. So, the first signal would have to come >from the outside... >This probably IS a stupid problem, but could someone please give me an >answer ? >Thanks >Helder M. Vieira Hi, I am not a neuro or developmental biologist but I do know that the human egg is not a homogenous cell. Gradients already exist within the egg called the "animal" and "vegtable" poles. It is these pre-set poles that determine body axis patterning (I beleive the animal will eventualy become the head). This information should be available in any text book on development... I hope this helps, Gary Morley gmorley@rpms.ac.uk From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!pravda.ucr.edu!awabi.library.ucla.edu!208.134.241.18!newsfeed.internetmci.com!204.59.152.222!news-peer.gip.net!news-stkh.gip.net!news.gsl.net!gip.net!newsfeed1.funet.fi!128.214.205.17.MISMATCH!news.helsinki.fi!not-for-mail From: stenberg@cc.helsinki.nospam.fi Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc,sci.bio.microbiology,sci.bio.technology Subject: Re: Bioscience Humour is on the Web Date: 4 Jun 1998 08:35:43 GMT Organization: University of Helsinki Lines: 19 Message-ID: <6l5m8v$hqb$1@oravannahka.Helsinki.FI> References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <3573e0ca.2593980@news.gatech.edu> <357538BD.5723DCF3@mindless.com> <35764606.BC6@nospam.ic.ac.uk> NNTP-Posting-Host: vesuri.helsinki.fi X-Newsreader: TIN [UNIX 1.3 unoff BETA 970613; alpha OSF1 V4.0] Xref: biosci bionet.molbio.methds-reagnts:67962 bionet.molbio.proteins:12870 bionet.general:30096 In bionet.general Koen De Smet wrote: > Roney Graf wrote: > > In article <357538BD.5723DCF3@mindless.com>, Andy > > wrote: > > > The page loads but as soon as the "little gladiator?" starts walking - > > > My system crashes too! (NS4.07) > > Net$cape 2.02. Apple Mac LC, 10MB, System 7.1. Not exactly the most > > advanced of configurations. No problems. > Netscape 2.02. Apple Mac 6100/60, 16MB, System 7.5. Should be more > advanced than the above, but it crashed on accessing this page. Mind > you, it often crashes while accessing certain sites, so now I have a > list of pages that have a high likelyhood of crashing (including > home.netscape!) And my Netscape 4.04 under Windows 95 crashed too. Dag Stenberg From owner-proteins@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.direct.ca!btnet-peer!btnet!dispose.news.demon.net!demon!news.demon.co.uk!demon!reeveltd.demon.co.uk!d_reeve From: David Reeve Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,sci.chem.analytical Subject: Re: Stable Amino Acid Derivative for HPLC? Date: Thu, 4 Jun 1998 09:51:46 +0100 Organization: Reeve Analytical Ltd Distribution: world Message-ID: References: NNTP-Posting-Host: reeveltd.demon.co.uk X-NNTP-Posting-Host: reeveltd.demon.co.uk:158.152.103.245 X-Trace: news.demon.co.uk 896960333 nnrp-09:16351 NO-IDENT reeveltd.demon.co.uk:158.152.103.245 X-Complaints-To: abuse@demon.net MIME-Version: 1.0 X-Newsreader: Turnpike Version 3.04 Lines: 22 Xref: biosci bionet.molbio.methds-reagnts:67965 bionet.molbio.proteins:12872 Aren't dansyl derivatives reasonably stable? I seem to remember seeing purified dansyl-amino acids in the Sigma catalogue. Dave In article , Phil Calvert writes >There a number of methods available for derivatizing amino acids and >analyzing them by reverse-phase HPLC on a C18 column. Unfortunately, many >of these derivatives are not very stable. Obviously, they are stable >enough for analytical purposes. But I am interested in doing preparative >HPLC and isolating the purified amino acid derivatives; so if the >derivatives aren't very stable, that sort of defeats the purpose. > >I would like to use a UV detector for detecting the derivatized amino >acids. Therefore, derivatives that are UV-absorbing (including fluorescent >derivatives) are preferred (provided that they are stable). Anyone know of >any derivatives that fit these qualifications? > > >Phil Calvert > From owner-proteins@net.bio.net Thu Jun 04 23:00:00 1998 Path: biosci!PIERRE.HH.RI.CCF.ORG!yee From: yee@PIERRE.HH.RI.CCF.ORG (Vivien Chia Yee) Newsgroups: bionet.molbio.proteins Subject: Postdoctoral Position Date: 5 Jun 1998 07:52:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: <35780598.167E@pierre.hh.ri.ccf.org> NNTP-Posting-Host: net.bio.net A postdoctoral position in protein crystallography is available in the Department of Molecular Cardiology at the Lerner Research Institute of the Cleveland Clinic Foundation. Applicants should hold a Ph.D. in biochemistry, chemistry, or a related field; prior experience in macromolecular crystallography or in molecular biology and protein expression is required. The successful applicant will be involved in several projects probing enzyme structure and function. Diffracting crystals of one protein, a subunit in the carboxylase multienzyme complex, have been grown; other proteins are available for crystallization. The laboratory is fully equipped for protein expression and purification, and macromolecular crystallography. Our group is one of several new structural biology research labs in the Lerner Research Institute, which houses about 100 independent laboratories and is the research arm of the renowned Cleveland Clinic Foundation. This postdoctoral position is available immediately; applications will be accepted until the position is filled. Experienced macromolecular crystallographers or molecular biologists/protein chemists interested in expanding into crystallographic work will be seriously considered. Salary will be commensurate with qualifications and experience. Interested candidates should send a cover letter, c.v. and list of three references to: Vivien Yee Molecular Cardiology / FF10 Lerner Research Institute Cleveland Clinic Foundation 9500 Euclid Avenue Cleveland, OH, USA 44195 email: yee@pierre.hh.ri.ccf.org phone: 1-216-444-2057 fax: 1-216-445-6062 The Cleveland Clinic Foundation is an Equal Opportunity/Affirmative Action Employer. From owner-proteins@net.bio.net Thu Jun 04 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!sunqbc.risq.qc.ca!torn!resunix.sickkids.on.ca!not-for-mail From: Randall C Willis Newsgroups: bionet.molbio.proteins Subject: Re: Protease inhibitor cocktail Date: Fri, 05 Jun 1998 10:26:59 -0400 Organization: The Hospital For Sick Children Lines: 41 Message-ID: <35780032.41C6@gandalf.psf.sickkids.on.ca> References: NNTP-Posting-Host: gandalf.sickkids.on.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.3 IP17) To: Shinhan Shiu Shinhan Shiu wrote: > My question is: are you aware of a recipe where you mix everything up > like a "protease cocktail"? Shinhan, If you're not too picky on the inhibitor content, Sigma sells a cocktail that is resuspended in DMSO and has been tailored for a number of cell systems (ie bacterial, eukaryotic...). As well, Boehringer Mannheim sells a system called Complete (+/- EDTA) which comes in little tablets. As a first step, the cocktails aren't too bad but can become expensive. For my bacterial work, I like to use the Complete system for the initial cell lysis and first column but at concentrations of one half to one quarter of what B-M recommends for the purposes of keeping costs down and it is so far working like a charm. Good luck, Randall C Willis, Researcher Biochemistry Research, Hospital for Sick Children 3421-555 University Ave Toronto, ON M5G 1X8 CANADA 416-813-5933 (ph) -5022 (fax) willis@gandalf.psf.sickkids.on.ca Randall C Willis, Publisher Aliquotes Press Aliquotes: A Journal Of Molecular and Biochemical Humour and Information 441 Sandlewood Rd. Oakville, ON L6L 3S3 CANADA 905-469-9189 (ph) 416-813-5022 (fax) roger@xybercom.net http://members.tripod.com/~BioHazard5/ From owner-proteins@net.bio.net Thu Jun 04 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gip.net!news-raspail.gip.net!news.gsl.net!gip.net!rain.fr!wanadoo.fr!not-for-mail From: lobo Newsgroups: bionet.molbio.proteins Subject: polymorphism Date: Fri, 05 Jun 1998 21:09:41 +0200 Organization: one Lines: 3 Message-ID: <35784275.445B@smtp.wanadoo.fr> Reply-To: roland.bonello@wanadoo.fr NNTP-Posting-Host: marse2-80.abo.wanadoo.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 [fr] (Macintosh; I; 68K) what explanation could ,you give to polymorphism in the evolution . Have you heard about polimorphism that could give an hypersensitivity to glucocorticoids,what could be the final result obtained? From owner-proteins@net.bio.net Thu Jun 04 23:00:00 1998 Path: biosci!pravda.ucr.edu!awabi.library.ucla.edu!208.134.241.18!newsfeed.internetmci.com!199.0.154.208!ais.net!iagnet.net!iagnet.net!news.uiowa.edu!vetplus.int-med.uiowa.edu!user From: alb2@cornell.edu (Allan Berger) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.general,sci.bio.misc,sci.bio.microbiology,sci.bio.technology Subject: Re: Aliquotes Crashes (WAS: Bioscience Humour is on the Web) Date: Fri, 05 Jun 1998 16:05:40 -0600 Organization: Cornell University Lines: 17 Message-ID: References: <3572C4F4.41C6@gandalf.psf.sickkids.on.ca> <6l0cq7$ce4_001@gene.le.ac.uk> <3573e0ca.2593980@news.gatech.edu> <357538BD.5723DCF3@mindless.com> <35754F55.41C6@gandalf.psf.sickkids.on.ca> NNTP-Posting-Host: vetplus.int-med.uiowa.edu X-Newsreader: Value-Added NewsWatcher 2.0b24.0+ Xref: biosci bionet.molbio.methds-reagnts:68002 bionet.molbio.proteins:12883 bionet.general:30106 In article <35754F55.41C6@gandalf.psf.sickkids.on.ca>, Randall C Willis wrote: > Are there others out there who are not having a problem and with what > browser and software? Netscape 4.04 on Mac G3 desktop works fine... AB Allan Berger Dept. of Internal Medicine University of Iowa, 500 EMRB Iowa City, IA 52242 (319) 335-6540 (voice) (319) 335-7623 (fax) From owner-proteins@net.bio.net Thu Jun 04 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!howland.erols.net!fu-berlin.de!oekotoxiv.biologie.fu-berlin.DE!not-for-mail From: Karin Zerulla Newsgroups: bionet.molbio.proteins Subject: mass spectroscopy Date: Fri, 05 Jun 1998 17:36:47 +0200 Organization: Freie Universitaet Berlin Lines: 19 Message-ID: <3578108B.F6A28053@zedat.fu-berlin.de> NNTP-Posting-Host: oekotoxiv.biologie.fu-berlin.de (160.45.212.137) Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Access: 16 17 19 X-Trace: fu-berlin.de 897060999 13444 (none) 160.45.212.137 X-Mailer: Mozilla 4.01 [de] (WinNT; I) X-Priority: 3 (Normal) Hi netters! I'm trying to purify a DNA-binding protein. After ion exchange chromatography I'm using Dynabeads as the last purification step. The DNA-binding activity is clearly detectable in the eluate (EMSAs). My problem is that there is no band detectable in protein gels (silverstain). I heard that it is possible to test samples with low protein quantity with the method of mass spectroscopy, but the opinions seem to differ. Someone said it's senseless, if I can't detect a band with silverstaining. In this newsgroup I read that it's possible to sequence quantities from femtomolar to picomolar quantities. Since I have no idea about the method I would greatly appreciate any comments. Which quantities can be measured? Is it possible to find out how many different proteins are in the eluate? Thank you very much in advance! Karin Zerulla From owner-proteins@net.bio.net Sat Jun 06 23:00:00 1998 Path: biosci!agate!newsfeed.wli.net!newshub.northeast.verio.net!fu-berlin.de!oekotoxiv.biologie.fu-berlin.DE!not-for-mail From: Karin Zerulla Newsgroups: bionet.molbio.proteins Subject: Re: Protease inhibitor cocktail Date: Sun, 07 Jun 1998 13:45:40 +0200 Organization: Freie Universitaet Berlin Lines: 25 Message-ID: <357A7D61.A28A6F33@zedat.fu-berlin.de> NNTP-Posting-Host: oekotoxiv.biologie.fu-berlin.de (160.45.212.137) Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Access: 16 17 19 X-Trace: fu-berlin.de 897219936 23864 (none) 160.45.212.137 X-Mailer: Mozilla 4.01 [de] (WinNT; I) X-Priority: 3 (Normal) > Shinhan Shiu wrote: > > My question is: are you aware of a recipe where you mix everything > up > > like a "protease cocktail"? > > > Shinhan, > > If you're not too picky on the inhibitor content, Sigma sells a > cocktail > that is resuspended in DMSO and has been tailored for a number of cell > systems (ie bacterial, eukaryotic...). As well, Boehringer Mannheim > sells a system called Complete (+/- EDTA) which comes in little > tablets. > Just a word to Complete: Right now Boehringer offers free samples (till June 15th). Ordering information: Complete Mini 1 836 153 Complete Mini EDTA-free 1 836 170 If you like to try it... Best wishes, Karin From owner-proteins@net.bio.net Sat Jun 06 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!logbridge.uoregon.edu!newsfeed.ecrc.net!news-fra1.dfn.de!news-koe1.dfn.de!news-han1.dfn.de!newsserver.rrzn.uni-hannover.de!not-for-mail From: "Christian Kaiser" Newsgroups: bionet.molbio.proteins Subject: Michaelis constant of Creatine-Kinase Date: Sun, 7 Jun 1998 18:30:02 +0200 Organization: RRZN - Newsserver Lines: 15 Message-ID: <6lf079$luq$1@newsserver.rrzn.uni-hannover.de> NNTP-Posting-Host: h55.stud.uni-hannover.de X-Newsreader: Microsoft Outlook Express 4.71.1712.3 X-MimeOLE: Produced By Microsoft MimeOLE V4.71.1712.3 Hello! Does anybody know the Michaelis constant of creatine kinase isolated from pig skeletal muscle or the adress of an internet-site to look it up? (The constant for a CK-MM from any other species will do, too.) Thanks a lot C. Kaiser From owner-proteins@net.bio.net Sun Jun 07 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!sunqbc.risq.qc.ca!news.uow.edu.au!metro!munnari.OZ.AU!news.unimelb.edu.au!ludwignt-4 From: murphy_r@licre.ludwig.edu.au (Roger Murphy) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,sci.chem.analytical Subject: Re: Stable Amino Acid Derivative for HPLC? Date: Tue, 09 Jun 98 00:57:03 GMT Organization: Ludwig Institute for Cancer Research Lines: 48 Message-ID: <6li4lh$dl5$1@izvestia.its.unimelb.edu.au> References: NNTP-Posting-Host: ludwignt-4.austin.unimelb.edu.au X-Newsreader: News Xpress 2.0 Beta #0 Xref: biosci bionet.molbio.methds-reagnts:68048 bionet.molbio.proteins:12890 Fmoc derivatives of amino acids are very stable, hence their use in peptide synthesis. You also have a high absorbing chromophore for good sensititvity. Fmoc derivatization is also now the basis of a number of commercial amino acid analysis systems. Cheers, Roger In article , calvert@eos.ncsu.edu (Phil Calvert) wrote: >There a number of methods available for derivatizing amino acids and >analyzing them by reverse-phase HPLC on a C18 column. Unfortunately, many >of these derivatives are not very stable. Obviously, they are stable >enough for analytical purposes. But I am interested in doing preparative >HPLC and isolating the purified amino acid derivatives; so if the >derivatives aren't very stable, that sort of defeats the purpose. > >I would like to use a UV detector for detecting the derivatized amino >acids. Therefore, derivatives that are UV-absorbing (including fluorescent >derivatives) are preferred (provided that they are stable). Anyone know of >any derivatives that fit these qualifications? > > >Phil Calvert > >-- >------------------------------------ >!-----------Phil Calvert-----------! >!---calvert.NoSpam@eos.ncsu.edu----! >------------------------------------ >NOTE: If present, the phrase ".NoSpam" must be removed from my >return address before sending your reply. Roger Murphy, Ph.D. Biological Production Facility Ludwig Institute for Cancer Research Austin & Repatriation Medical Centre Studley Road, Heidelberg, Vic. 3084 Australia. Tel 61-3-94965463 Fax 61-3-94965436 Email murphy_r@licre.ludwig.edu.au From owner-proteins@net.bio.net Sun Jun 07 23:00:00 1998 Path: biosci!agate!howland.erols.net!feed2.news.erols.com!erols!not-for-mail From: Joan Evans Newsgroups: bionet.molbio.proteins Subject: Life Science Vendor Service & Support Date: Mon, 08 Jun 1998 08:47:16 +0000 Organization: The Science Advisory Board Lines: 27 Message-ID: <357BA515.1124@scienceboard.net> Reply-To: j.evans@scienceboard.net NNTP-Posting-Host: 207-172-111-5.s5.tnt1.ann.erols.com Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Trace: winter.news.erols.com 897309649 25097 207.172.111.5 (8 Jun 1998 12:40:49 GMT) X-Complaints-To: abuse@erols.com X-Mailer: Mozilla 3.02Gold (Macintosh; I; PPC) This week (June 8, 1998), The Science Advisory Board (http://www.scienceboard.net) will begin a new study measuring satisfaction with the customer service and technical support offered by life science vendors. The availability of responsive, helpful and knowledgeable service and support is a major factor in the success of one’s research. The efficiency and effectiveness with which service and support are delivered, however, can vary greatly from vendor to vendor. The results of this study will be used by industry to improve their service and support departments to better meet the needs and expectations of the life sciences community. The Science Advisory Board is an online panel of more than 3,400 scientists, physicians and other medical professionals from 64 countries. The Board convenes electronically to participate in online surveys and focus groups to voice their opinions on a wide variety of topics related to new research products and emerging technologies. If you would like to participate in this, or future studies, please complete the registration form which can be found at http://www.scienceboard.net. Your identity and individual responses are always held in strict confidence, and participants are compensated for their time. Joan Evans Membership Secretary The Science Advisory Board http://www.scienceboard.net From owner-proteins@net.bio.net Sun Jun 07 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!ix.netcom.com!news From: Mark Atlas Newsgroups: bionet.molbio.proteins Subject: Lab equipment auctions online with Going, Going... Sold! Date: Mon, 08 Jun 1998 23:42:14 GMT Organization: ICGNetcom Lines: 126 Message-ID: <6lhspg$qao@dfw-ixnews3.ix.netcom.com> NNTP-Posting-Host: sji-ca4-45.ix.netcom.com X-NETCOM-Date: Mon Jun 08 6:40:32 PM CDT 1998 X-Newsreader: NETCOMplete/4.0 Lab equipment auctions online with Going, Going... Sold! Join the excitement. Going, Going Sold has sold equipment ranging from microscopes to ICP's through the Internets most convenient and safest way to purchase used lab equipment. Why do we say this and why do people spend money on equipment on the Internet. Going, going… Sold!! Utilizes an in lab evaluation period created through a customized escrow specifically designed for lab equipment evaluations. Many people utilize this process over conventional means as it actually provides greater safety than conventional purchasing from unknown parties. Come visit http://www.going-going-sold.com new features include the custom equipment search service and leasing to own programs. Sell off your surplus with no hassles. The following Items are scheduled to go on Auction over the next few weeks. ***************************************************** Sale Closing : 6/12/98 Opening Prices ***************************************************** 1. automatic abbe refractometer $2,100.00 ------------------------------------------------------------ 2. Beckman DU-7 $3,500.00 ------------------------------------------------------------ 3. HP MS Engine/Electrospray System $63,000.00 ------------------------------------------------------------ 4. HP 5890 GC $12,000.00 ------------------------------------------------------------ 5. New Olympus Microscope $1,600.00 ------------------------------------------------------------ 6. ICP $8,500.00 ------------------------------------------------------------ 7. Kjeldahl ( Macro) Distillation Unit $1,800.00 ------------------------------------------------------------ 8. Laboratory Furniture $7,000.00 ------------------------------------------------------------ 9. PE AS 90 Autosampler $4,700.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 6/17/98 Opening Prices ***************************************************** 1. Waters 717 autosampler $4,700.00 ------------------------------------------------------------ 2. Environmental Chamber $5,250.00 ------------------------------------------------------------ 3. Phase Contrast Microscope $2,000.00 ------------------------------------------------------------ 4. ABEC Bioreactor $77,500.00 ------------------------------------------------------------ 5. Gruenburg Depyro Oven $1,700.00 ------------------------------------------------------------ 6. Seperations Technologies Prep Bar-1000 $48,000.00 ------------------------------------------------------------ 7. Grass Inst. Recorder/Polygraph $6,500.00 ------------------------------------------------------------ 8. Hitachi Z-9000 $15,750.00 ------------------------------------------------------------ 9. Perkin Elmer Plasma 400 Spec $21,000.00 ------------------------------------------------------------ 10. Zeiss EM 10 Transmitting Electron Microscope $22,750.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 6/19/98 Opening Prices ***************************************************** 1. Model 996 PDA Detector $4,750.00 ------------------------------------------------------------ 2. Capillary Rheometer System $4,750.00 ------------------------------------------------------------ 3. Inductively Coupled Plasma $19,400.00 ------------------------------------------------------------ 4. Graphite Furnace AA 5100ZL $24,300.00 ------------------------------------------------------------ 5. Microwave Digestor $1,800.00 ------------------------------------------------------------ 6. BOD Incubator $2,300.00 ------------------------------------------------------------ 7. Laminar Flow Hood $1,750.00 ------------------------------------------------------------ 8. Polarizing Microscope $1,900.00 ------------------------------------------------------------ 9. Leica Microscope $1,500.00 ------------------------------------------------------------ 10. HP Supercritical Fluid Extraction $23,500.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 6/24/98 Opening Prices ***************************************************** 1. Sorvall RC 3B and Rotor $7,400.00 ------------------------------------------------------------ 2. Centra 4B $1,395.00 ------------------------------------------------------------ 3. Tangential Flow System $15,000.00 ------------------------------------------------------------ 4. Beckman GPR Centrifuge $3,100.00 ------------------------------------------------------------ 5. Beckman L5-75 Centrifuge& Rotors $6,900.00 ------------------------------------------------------------ 6. Beckman DU-62 $4,200.00 ------------------------------------------------------------ 7. Gilson HPLC System $5,450.00 ------------------------------------------------------------ 8. Beta Scint. Counter $13,000.00 ------------------------------------------------------------ 9. Beckman DU-70 $4,900.00 ------------------------------------------------------------ 10. Waters GPC system $10,500.00 ------------------------------------------------------------ 11. Bio-safety hood 4 foot with stand Type IIA, or IIB $2,200.00 ------------------------------------------------------------ 12. IR Spectrometer $10,000.00 ------------------------------------------------------------ 13. Fluoresence Microscope $4,800.00 ------------------------------------------------------------ ***************************************************** (c) 1997, Internet Auctioneers International From owner-proteins@net.bio.net Sun Jun 07 23:00:00 1998 Path: biosci!MANI.CBS.UMN.EDU!npv From: npv@MANI.CBS.UMN.EDU (Nora Plesofsky-Vig) Newsgroups: bionet.molbio.proteins Subject: NMR analysis of glucose metabolism Date: 8 Jun 1998 17:21:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <9806090114.AA00421@mani.cbs.umn.edu> Reply-To: nora@biosci.cbs.umn.edu NNTP-Posting-Host: net.bio.net This is not exactly the right subject for this group, but any information would be appreciated. Does anyone know a source that assigns specific NMR peaks (in 13C-analysis) to specific carbons of glucose? I can find (and have determined) the peaks for glucose and [13C-1]glucose, but I would like to know specifically where the peaks for 13C in the other five positions of glucose would appear. Thanks. Nora Plesofsky nora@biosci.cbs.umn.edu From owner-proteins@net.bio.net Mon Jun 08 23:00:00 1998 Newsgroups: bionet.molbio.proteins Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!ais.net!ix.netcom.com!atlantis From: atlantis@netcom.com (JJ Miranda) Subject: Re: ? Peptide solubilisation ? Message-ID: Organization: Netcom On-Line Services X-Newsreader: TIN [version 1.2 PL2] References: <357D17B1.4C3BB37F@student.mahidol.ac.th> Date: Tue, 9 Jun 1998 22:15:36 GMT Lines: 34 Sender: atlantis@netcom.netcom.com Dear Jongrak, If the peptide is really hydrophobic (I'm not sure what concentration of organic you tried in the other solvents), you might want to try to dissolve with a little detergent like SDS or octyl-glucoside. JJ Jongrak Kittiworakarn (g3937506@STUDENT2.MAHIDOL.AC.TH) wrote: : Dear All: : I am going to purify a synthetic peptide by hplc, but I have a problem : with its solubility. : I have tried several solvent, i.e. CH3CN, water, 20-50% CH3CN in water, : isopropanol, methanol, DMF, but the peptide just slightly soluble in : those solvent. : I would greatly appreciate, if you can give me any suggestion on the : solubilisation of the peptide. : Thanks in advance : Jongrak : -- : -----------------------------------Mr. Jongrak Kittiworakarn : g3937506@student.mahidol.ac.th : Inst. of Science and Technology for Research and Development : Mahidol University (Salaya Campus) : Salaya, Nokornprathom, THAILAND. : Tel. 001-66-2-441-9003-7 ext.1277,1278,1279 : Fax. 001-66-2-441-9906, 001-66-2-441-1013 : ----------------------------------------------------------------- From owner-proteins@net.bio.net Mon Jun 08 23:00:00 1998 Path: biosci!STUDENT2.MAHIDOL.AC.TH!g3937506 From: g3937506@STUDENT2.MAHIDOL.AC.TH (Jongrak Kittiworakarn) Newsgroups: bionet.molbio.proteins Subject: ? Peptide solubilisation ? Date: 9 Jun 1998 04:14:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <357D17B1.4C3BB37F@student.mahidol.ac.th> NNTP-Posting-Host: net.bio.net Dear All: I am going to purify a synthetic peptide by hplc, but I have a problem with its solubility. I have tried several solvent, i.e. CH3CN, water, 20-50% CH3CN in water, isopropanol, methanol, DMF, but the peptide just slightly soluble in those solvent. I would greatly appreciate, if you can give me any suggestion on the solubilisation of the peptide. Thanks in advance Jongrak -- -----------------------------------Mr. Jongrak Kittiworakarn g3937506@student.mahidol.ac.th Inst. of Science and Technology for Research and Development Mahidol University (Salaya Campus) Salaya, Nokornprathom, THAILAND. Tel. 001-66-2-441-9003-7 ext.1277,1278,1279 Fax. 001-66-2-441-9906, 001-66-2-441-1013 ----------------------------------------------------------------- From owner-proteins@net.bio.net Mon Jun 08 23:00:00 1998 Newsgroups: bionet.molbio.proteins Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!128.174.5.49!vixen.cso.uiuc.edu!uchinews!midway.uchicago.edu!aekentsi From: aekentsi@midway.uchicago.edu (alex kentsis) Subject: Re: ? Peptide solubilisation ? X-Nntp-Posting-Host: howard-nfs.uchicago.edu Message-ID: Sender: news@midway.uchicago.edu (News Administrator) Organization: The University of Chicago X-Newsreader: TIN [version 1.2 PL2] References: <357D17B1.4C3BB37F@student.mahidol.ac.th> Date: Tue, 9 Jun 1998 14:13:53 GMT Lines: 29 what is the sequence of your peptide? Jongrak Kittiworakarn (g3937506@STUDENT2.MAHIDOL.AC.TH) wrote: : Dear All: : I am going to purify a synthetic peptide by hplc, but I have a problem : with its solubility. : I have tried several solvent, i.e. CH3CN, water, 20-50% CH3CN in water, : isopropanol, methanol, DMF, but the peptide just slightly soluble in : those solvent. : I would greatly appreciate, if you can give me any suggestion on the : solubilisation of the peptide. : Thanks in advance : Jongrak : -- : -----------------------------------Mr. Jongrak Kittiworakarn : g3937506@student.mahidol.ac.th : Inst. of Science and Technology for Research and Development : Mahidol University (Salaya Campus) : Salaya, Nokornprathom, THAILAND. : Tel. 001-66-2-441-9003-7 ext.1277,1278,1279 : Fax. 001-66-2-441-9906, 001-66-2-441-1013 : ----------------------------------------------------------------- From owner-proteins@net.bio.net Tue Jun 09 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.sprintlink.net!news.sprintlink.net!newsfeed.nacamar.de!blackbush.xlink.net!rz.uni-karlsruhe.de!news.urz.uni-heidelberg.de!usenet From: Rico Laage Newsgroups: bionet.molbio.proteins Subject: french press Date: Wed, 10 Jun 1998 12:28:37 +0100 Organization: Department of Neurobiology Lines: 27 Message-ID: <357E6DE0.D2A8BF07@urz.uni-heidelberg.de> Reply-To: rico.laage@urz.uni-heidelberg.de NNTP-Posting-Host: 129.206.50.233 Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: quoted-printable X-Mailer: Mozilla 4.05 (Macintosh; I; PPC) Dear colleagues I=B4m starting to use a french press for breaking my bugs to purify a recombinant protein, I have a problem what pressure I should use for E. coli to completely crack the cells- I am operating aSLM Aminco with the 40K manual fill cell- thanx a lot for any suggestions- -- *************************************************************************= **************** Rico Laage Neurobiology University of Heidelberg Im Neuenheimer Feld 364 69120=A0Heidelberg Germany Voice +49=A0(6221)54-4863 Fax +49=A0(6221)54-4496 World-Wide-Web: http://server.nbio.uni-heidelberg.de/Neurobiology.html http://server.nbio.uni-heidelberg.de/Groups/WWW_Langosch/Rico_Laage.html *************************************************************************= ***************** From owner-proteins@net.bio.net Tue Jun 09 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!baron.netcom.net.uk!netcom.net.uk!nntp.news.xara.net!xara.net!interpath.net!news-relay.ncren.net!fddinewz.oit.unc.edu!usenet From: Pat Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins Subject: Request plasmid Date: Wed, 10 Jun 1998 10:59:12 -0400 Organization: The University of North Carolina at Chapel Hill Lines: 10 Message-ID: <357E9F40.5ABE@med.unc.edu> NNTP-Posting-Host: pc206h256.med.unc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win95; I) Xref: biosci bionet.molbio.methds-reagnts:68108 bionet.molbio.proteins:12896 Hello out there-- I'm posting to ask if anyone out there has any pcDNA3.1(-) vector that they would be willing to send me. I have access to the plus form but I need the MCS reversed and I just can't see dropping another $220 for it. I appreciate anyone's help. Thanks, Donelson Smith UNC-CH Dept. of Physiology racerx@med.unc.edu From owner-proteins@net.bio.net Wed Jun 10 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!netnews.com!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: thierry.baussant@pierre-fabre.com Newsgroups: bionet.molbio.proteins Subject: Re: ? Peptide solubilisation ? Date: Thu, 11 Jun 1998 07:02:24 GMT Organization: Deja News - The Leader in Internet Discussion Lines: 26 Message-ID: <6lnve0$h50$1@nnrp1.dejanews.com> References: <357D17B1.4C3BB37F@student.mahidol.ac.th> NNTP-Posting-Host: 194.250.96.21 X-Article-Creation-Date: Thu Jun 11 07:02:24 1998 GMT X-Http-User-Agent: Mozilla/2.0 (compatible; MSIE 3.0; Windows 95) In article <357D17B1.4C3BB37F@student.mahidol.ac.th>, g3937506@STUDENT2.MAHIDOL.AC.TH (Jongrak Kittiworakarn) wrote: > > Dear All: > > I am going to purify a synthetic peptide by hplc, but I have a problem > with its solubility. > I have tried several solvent, i.e. CH3CN, water, 20-50% CH3CN in water, > isopropanol, methanol, DMF, but the peptide just slightly soluble in > those solvent. > I would greatly appreciate, if you can give me any suggestion on the > solubilisation of the peptide. > > Thanks in advance > Jongrak You can use a low molar base (NaOH 10 mM in water) for a short time in order to prevent the peptide degradation. Yours Thierry Baussant www.cipf.com -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/ Now offering spam-free web-based newsreading From owner-proteins@net.bio.net Wed Jun 10 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-fra.maz.net!news-fra1.dfn.de!news-ber1.dfn.de!news-lei1.dfn.de!news.uni-leipzig.de!news.uni-halle.de!not-for-mail From: Frederik Boernke Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins Subject: Re: Request plasmid Date: Thu, 11 Jun 1998 09:22:27 -0700 Organization: IPK Gatersleben Lines: 35 Message-ID: <35800443.751@nospam.ipk-gatersleben.de> References: <357E9F40.5ABE@med.unc.edu> Reply-To: boernke@nospam.ipk-gatersleben.de NNTP-Posting-Host: mpp-14.ipk-gatersleben.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.03 (Win16; I) To: Pat Xref: biosci bionet.molbio.methds-reagnts:68132 bionet.molbio.proteins:12898 -- Pat wrote: > > Hello out there-- > I'm posting to ask if anyone out there has any pcDNA3.1(-) > vector that they would be willing to send me. I have access to > the plus form but I need the MCS reversed and I just can't see dropping > another $220 for it. I appreciate anyone's help. > > Thanks, > Donelson Smith > UNC-CH Dept. of Physiology > racerx@med.unc.edu Hi! Maybe you should give this URL a try: http://shigen.lab.nig.ac.jp/cvector.html It was posted to the group yesterday. I took a quick look at it and it seems as they really got everything what one deserves. Good luck Ricky ****************************************************************** Frederik Boernke Research Group of Molecular Plant Physiology Institute for Plant Genetics and Crop Plant Research (IPK) Corrensstr. 3 06466 Gatersleben Tel. 039482 -5 321 Fax. 039482 -5 515 http://www.ipk-gatersleben.de From owner-proteins@net.bio.net Wed Jun 10 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.wli.net!howland.erols.net!surfnet.nl!news-ge.switch.ch!news-zh.switch.ch!elna.ethz.ch!tan From: tan@mol.biol.ethz.ch (Song Tan) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins Subject: reagent for titering baculovirus Date: Thu, 11 Jun 1998 16:52:19 +0200 Organization: ETH-Honggerberg (Swiss Federal Inst of Technology) Lines: 12 Message-ID: NNTP-Posting-Host: smac.ethz.ch Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Yet Another NewsWatcher 2.4.0 Xref: biosci bionet.molbio.methds-reagnts:68141 bionet.molbio.proteins:12899 I vaguely remembering seeing an ad describing a reagent (probably an antibody) to speed up determination of baculoviral titers. Problem is, I don't remember where I saw the ad and I can't remember the company. Could anyone who remembers the ad please give me a lead, even better, could someone who has tried it please rate the product? Many thanks! -- Song Tan ETH-Honggerberg (Swiss Federal Institute of Technology) CH-8093 Zurich, Switzerland From owner-proteins@net.bio.net Wed Jun 10 23:00:00 1998 Path: biosci!agate!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!news.mel.connect.com.au!munnari.OZ.AU!news.unimelb.edu.au!ludwignt-4 From: murphy_r@licre.ludwig.edu.au (Roger Murphy) Newsgroups: bionet.molbio.proteins Subject: Re: ? Peptide solubilisation ? Date: Fri, 12 Jun 98 03:40:07 GMT Organization: Ludwig Institute for Cancer Research Lines: 45 Message-ID: <6lqbam$9cu$1@izvestia.its.unimelb.edu.au> References: <357D17B1.4C3BB37F@student.mahidol.ac.th> NNTP-Posting-Host: ludwignt-4.austin.unimelb.edu.au X-Newsreader: News Xpress 2.0 Beta #0 I would not advise using long chain detergents such as SDS - you will never get them off the reversed phase column! DMSO is a useful solvent for hydrophobic peptides, or you might try dissolving in a little neat TFA (say 5% of fianl volume) and then diluting with acetonitrile, methanol, DMSO or aqueous mixtures of these solvents Its hard to give advise without knowing the sequence of the peptide however.... Hope this helps, Roger In article , atlantis@netcom.com (JJ Miranda) wrote: >Dear Jongrak, > >If the peptide is really hydrophobic (I'm not sure what concentration of >organic you tried in the other solvents), you might want to try to >dissolve with a little detergent like SDS or octyl-glucoside. > >JJ > >Jongrak Kittiworakarn (g3937506@STUDENT2.MAHIDOL.AC.TH) wrote: >: Dear All: > >: I am going to purify a synthetic peptide by hplc, but I have a problem >: with its solubility. >Ä Roger Murphy, Ph.D. Biological Production Facility Ludwig Institute for Cancer Research Austin & Repatriation Medical Centre Studley Road, Heidelberg, Vic. 3084 Australia. Tel 61-3-94965463 Fax 61-3-94965436 Email murphy_r@licre.ludwig.edu.au From owner-proteins@net.bio.net Thu Jun 11 23:00:00 1998 Path: biosci!SSMAIN.UNISS.IT!naimi From: naimi@SSMAIN.UNISS.IT (naimi secretary) Newsgroups: bionet.molbio.proteins Subject: NAIMI congress Date: 12 Jun 1998 05:31:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5030 Sender: daemon@net.bio.net Distribution: world Message-ID: <199806121040.MAA08647@ssmain.uniss.it> NNTP-Posting-Host: net.bio.net --=====================_897640995==_ Content-Type: text/html; charset="us-ascii" Dear,

The University of Sassari, Sardinia, Italy, supports initiatives centred around the theme of scientific research in the framework of EU policies promoting transnational scientific co-operation among European and other countries. In particular, the University of Sassari is organising a round table entitled:

Scientific research in Mediterranean Countries in the framework of EU programmes:
current status and perspectives

The round table will take place on September 5th at the "Porto Conte Ricerche" centre, (Alghero, SS, Sardinia), as part of the NAIMI symposium (Nucleic Acids and their Interactions with Metal Ions), a satellite symposium of the ICCC Congress (International Conference on Coordination Chemistry - Florence).

We would be very glad if a representative of your Agency/Organisation would be interested in participating in the round table; we believe that this event will provide a good opportunity to exchange information on EU policies for the support of international research projects, and possibly offer some ideas on forms of co-operation between EU and non EU countries.

In order to further promote closer links between the world of research and Public Support Agencies, we are also forwarding an invitation to main Universities/Research Institutes from various countries to submit applications by a young scientist (under 35 years of age)to participate in the works of the NAIMI symposium.

We are looking forward to your kind reply, and are at your disposal for any further information you may request.

Yours sincerely

Prof. Maria Luisa Ganadu


For further information about NAIMI Congress, please contact naimi@ssmain.uniss.it
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