From owner-proteins@net.bio.net Sun May 02 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!remarQ73!supernews.com!remarQ.com!howland.erols.net!news.net.uni-c.dk!not-for-mail From: "Søren W. Rasmussen" Newsgroups: bionet.molbio.proteins Subject: Dnatools, revision 459 (with Clustal W) Date: Mon, 03 May 1999 09:32:11 +0200 Organization: UNI-C Lines: 47 Message-ID: <372D50FA.D1A07DCC@crc.dk> NNTP-Posting-Host: 130.226.182.97 Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Trace: news.net.uni-c.dk 925717363 29678 130.226.182.97 (3 May 1999 07:42:43 GMT) X-Complaints-To: usenet@news.net.uni-c.dk NNTP-Posting-Date: 3 May 1999 07:42:43 GMT X-Mailer: Mozilla 4.03 [en] (Win95; I) Dear DNATools user. The latest revision of DNATools now includes multi-sequence alignment with Clustal W (1.74). The Clustal program has been fully integrated into DNATools and can be accessed in the same way as other project related functions. Just click to select the sequences in your project you want to align and choose the output format. The Find Repeat function has been redesigned and now returns the 10 largest largest repeats (direct or inverted) found in the selected sequence. The Build Contig alignment routine has been refined - but still only work on the basis of the longest identical region between sub-sequences. The Merge Overlapping Forward and Reverse sequences (mainly relevant for EST projects) has been refined. All SAGE (serial analysis of gene expression) programs have been extensively tested and optimized. Especially the link between EST and SAGE data has been extended with several options for generating reports of matches between the two types of data. The Help and Help Index files have been updated. You can read more about DNATools (5.1.459) at: http://www.crc.dk/phys/A01B04_dnatools.htm download the new revision (5.1.459) at http://www.crc.dk/phys/Dnatools/A01B04C04_Downloading.htm and register your copy of DNATools at http://www.crc.dk/phys/Dnatools/A01B04C08_Registration.htm Regards Soeren -- Dr. scient. Søren W. Rasmussen Carlsberg Laboratory, Department of Physiology 10 Gl. Carlsbergvej, DK-2500, Copenhagen, Denmark Phone 45 3327 5230 / 45 3616 2259, Fax 45 3327 4766 E-mail swr@crc.dk, Homepage http://www.crc.dk/phys/ From owner-proteins@net.bio.net Sun May 02 23:00:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!dispose.news.demon.net!demon!news-feed.inet.tele.dk!bofh.vszbr.cz!newsfeed.tli.de!newsfeed.ecrc.net!newsrouter.chello.at!newsfeed03.univie.ac.at!news.univie.ac.at!not-for-mail From: a8803349.nospam@unet.univie.ac.at (Martin Offterdinger) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.cellbiol Subject: Re: Charcoal stripping FBS/FCS Date: Mon, 03 May 1999 10:31:13 GMT Organization: AKH Message-ID: <372d7a4b.4975600@news.univie.ac.at> References: NNTP-Posting-Host: grunt.imed1.akh-wien.ac.at X-Trace: www.univie.ac.at 925729125 33192 149.148.97.3 (3 May 1999 10:58:45 GMT) X-Complaints-To: news-adm@news.univie.ac.at NNTP-Posting-Date: 3 May 1999 10:58:45 GMT X-Newsreader: Forte Free Agent 1.1/16.230 Lines: 21 Xref: biosci bionet.molbio.methds-reagnts:75794 bionet.molbio.proteins:14290 bionet.cellbiol:11737 On Thu, 29 Apr 1999 20:08:19 +0100, i.mcfarlane@icrf.icnet.uk (Ian Mc) wrote: >Hi, > >Does anyone have a protocol for charcoal stripping foetal calf serum to >remove steriod hormones??? > >Thanks in advance, > >Ian Mc There several protocols around with stirring for 3d in the cold room extenisve filtration...., some use freon (extremely dangerous, liver toxic!!!)- a very messy procedure. We have stopped it a while ago and use a commercially stripped serum (Hyclone) Martin Offterdinger Internal Med.I,Dept. Oncology University of Vienna Austria E-Mail:a8803349.nospam@unet.univie.ac.at (remove .nospam before mailing) From owner-proteins@net.bio.net Sun May 02 23:00:00 1999 Path: biosci!WSUNIX.WSU.EDU!arthurr From: arthurr@WSUNIX.WSU.EDU (art roberts) Newsgroups: bionet.molbio.proteins Subject: Comprehensive Links to Various Databases Date: 3 May 1999 01:18:44 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <372D5B85.CB643B1A@wsunix.wsu.edu> NNTP-Posting-Host: net.bio.net http://biotech.isCool.net This site allows easy access to hyperlinks and free software for the biochemist, biophysicist, and molecular biologist. This site is constantly evolving and expanding, so it can be easy to use, reliable, and comprehensive. This is purely a non-profit site for your enjoyment. Sincerely, Art Roberts (web designer) From owner-proteins@net.bio.net Sun May 02 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!feeder.qis.net!yellow.newsread.com!netaxs.com!newsread.com!newsmonger.rutgers.edu!news-nb.rutgers.edu!amenti.rutgers.edu!not-for-mail From: meton@rci.rutgers.edu (Daniel Gonzalez) Newsgroups: bionet.molbio.proteins Subject: Symposium Green Fluorescent Protein GFP Date: 3 May 1999 16:37:05 -0400 Organization: Rutgers University Lines: 38 Sender: meton@amenti.rutgers.edu Message-ID: <7gl1dh$9dm$1@amenti.rutgers.edu> NNTP-Posting-Host: amenti.rutgers.edu X-Trace: newsmonger.rutgers.edu 925763805 16660 165.230.116.133 (3 May 1999 20:36:45 GMT) X-Complaints-To: news_support@email.rutgers.edu NNTP-Posting-Date: 3 May 1999 20:36:45 GMT Summary: Second International Green Fluorescent Protein Symposium Keywords: Second International Green Fluorescent Protein Symposium X-Newsreader: NN version 6.5.1 (NOV) GFP2 Announcements: The Webpage for the symposium has been updated and now has sessions and dates listed, see http://www.rci.rutgers.edu/~meton/GFP2.html. The cut-off for making reservations at the Town & Country (1-800-772-8527) is the 1st of May, after that date the special rate for attendees of our meeting may not be honoured, as the hotel will try to book the rooms reserved for our meeting to other travellers. The late registration deadline has been moved back to May 7th, for anyone registering from the internet off of our webpage, if anyone has any questions with this please contact Bill Ward (732-932-9071 ext. 216). Please consult the following table for an idea of our registration rates. Please note the discount for people from Rutgers, Scripps and UCSD. REGISTRATION RATES FOR UCSD, SCRIPPS, AND RUTGERS PERSONNEL ----------------------------------------------------------- STUDENTS (Full time students and postdocs) $150.00 FACULTY AND STAFF $297.50 Other registration rates after May 7 will be: REGULAR REGISTRATION RATES ----------------------------------------------------------- STUDENTS (Full time students and postdocs) $300.00 NON-PROFIT ORGANIZATIONS $595.00 CORPORATIONS AND FOR-PROFIT ORGANIZATIONS $895.00 Note: If you have registered and have not been confirmed withing 3 working days, contact us immediately so that we can expedite your registration. Those speakers who have not sent me an abstract and those people who wish to display posters at the meeting should go to the Symposium Website; http://www.rci.rutgers.edu/~meton/GFP2.html and go to the abstract page linked from that site; http://www.rci.rutgers.edu/~meton/Abstract.html to get the details for that. The deadline for which is May 7th. Daniel Gonzalez From owner-proteins@net.bio.net Mon May 03 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!dispose.news.demon.net!demon!newsfeed.icl.net!newsfeed.wirehub.nl!news.belnet.be!not-for-mail From: luc Newsgroups: bionet.molbio.proteins Subject: alk phos in screening Date: Tue, 04 May 1999 11:15:13 +0200 Organization: KULeuvenNet Message-ID: <372EBAA0.A5090AB9@sgi.celgen.kuleuven.ac.be> NNTP-Posting-Host: marvin.kulnet.kuleuven.ac.be Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: naxos.belnet.be 925809359 24156 134.58.127.3 (4 May 1999 09:15:59 GMT) X-Complaints-To: abuse@belnet.be NNTP-Posting-Date: 4 May 1999 09:15:59 GMT X-Mailer: Mozilla 4.5 [en] (WinNT; I) X-Accept-Language: en Cache-Post-Path: marvin!unknown@kiki.celgen.kuleuven.ac.be X-Cache: nntpcache 2.3.3 (see http://www.nntpcache.org/) Lines: 25 has anyone any experience with the use of alkaline phosphatase conjugated proteins in combination with chemiluminescence for expression cloning of e.g. receptors on the cell surface? as an alternative to radiolabeled ligand? we are strugling with high cellular alkphos background even after heating, levamisol, and other treatments. -- Luc Nelles VIB 07 department for cell growth, differentiation and development University of Leuven, Herestraat 49 3000 Leuven Email: luc@sgi.celgen.kuleuven.ac.be Tel. 32-16-345929 Fax. 32-16-345933 -- Luc Nelles VIB 07 department for cell growth, differentiation and development University of Leuven, Herestraat 49 3000 Leuven Email: luc@sgi.celgen.kuleuven.ac.be Tel. 32-16-345929 Fax. 32-16-345933 From owner-proteins@net.bio.net Tue May 04 23:00:00 1999 Path: biosci!SCRIPPS.EDU!jglma From: jglma@SCRIPPS.EDU (John Luz) Newsgroups: bionet.molbio.proteins Subject: protein interactions/salt Date: 5 May 1999 19:53:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <373102CA.6E28FAEF@scripps.edu> NNTP-Posting-Host: net.bio.net Hi Folks--I have overexpressed and purified two proteins. These two intracellular proteins interact constitutively. This has been demonstrated using whole cell lysates, immunoprecipitation, etc. I am trying to confirm the interaction in vitro. I incubated the two proteins for one hour on ice in 20 mM hepes pH 7.3/ 0.3 M KCl/ 10% glycerol. When applied to a gel filtration column the two proteins eluted separately and not as a complex. One of my proteins tends to aggregate in low ionic strength buffers, which is why I add KCl to 0.3M. But I am concerned that the salt concentration is too high for the complexes to form. Generally, speaking is this more salt than your average protein/protein interaction can handle? Also I would welcome any general comments regarding the experimental demonstration of in vitro protein interactions. Thanks.--john -- +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ John Gately Luz, Research Associate The Laboratory of Ian A. Wilson, D. Phil. The Department of Molecular Biology The Scripps Research Institute phone:(619)784-2945 10550 North Torrey Pines Road fax:(619)784-2980 La Jolla, California 92037 email:jglma@scripps.edu +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ From owner-proteins@net.bio.net Tue May 04 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!netnews.com!news-peer1.sprintlink.net!news-in-central.sprintlink.net!news.sprintlink.net!itssrv1.ucsf.edu!macmac-2.ucsf.edu!user From: bpmurray*STUFFER*@socrates.ucsf.edu (Bernard P. Murray, PhD) Newsgroups: bionet.molbio.proteins Subject: Re: protein interactions/salt Date: Wed, 05 May 1999 21:07:42 -0700 Organization: University of California, San Francisco Lines: 33 Distribution: world Message-ID: References: <373102CA.6E28FAEF@scripps.edu> NNTP-Posting-Host: macmac-2.ucsf.edu In article <373102CA.6E28FAEF@scripps.edu>, jglma@SCRIPPS.EDU (John Luz) wrote: > Hi Folks--I have overexpressed and purified two proteins. These two > intracellular proteins interact constitutively. This has been > demonstrated using whole cell lysates, immunoprecipitation, etc. I am > trying to confirm the interaction in vitro. > I incubated the two proteins for one hour on ice in 20 mM hepes pH 7.3/ > 0.3 M KCl/ 10% glycerol. When applied to a gel filtration column the > two proteins eluted separately and not as a complex. One of my proteins > tends to aggregate in low ionic strength buffers, which is why I add KCl > to 0.3M. But I am concerned that the salt concentration is too high > for the complexes to form. Generally, speaking is this more salt than > your average protein/protein interaction can handle? Also I would > welcome any general comments regarding the experimental demonstration of > in vitro protein interactions. Thanks.--john If an interaction can be broken with 0.3 M salt it is not very strong (I would be surprised that the immunoprecipitation worked). Any chance that either or both of your proteins forms homodimers? I was at a talk recently where the speaker could not get two candidates to bind to each other unless; a) they were coexpressed in the same cell, or b) they were denatured and renatured together. When this was done the heterodimer was very strong. Another possible problem is that there are "bridging" proteins active in vivo (or other proteins that stabilise the complex). Bernard -- Bernard P. Murray, PhD Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA From owner-proteins@net.bio.net Wed May 05 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!uchinews2!newsswitch.lcs.mit.edu!netnews.com!news-peer1.sprintlink.net!news-in-west1.sprintlink.net!news.sprintlink.net!news.ski.mskcc.org!z-suldan From: z-suldan@ski.mskcc.NOSPAM.org (z) Newsgroups: bionet.molbio.proteins,bionet.molbio.methds-reagnts Subject: Re: GST purification Date: Thu, 06 May 1999 16:08:42 -0500 Organization: z Lines: 17 Message-ID: References: NNTP-Posting-Host: msk-200-134.mskcc.org X-Newsreader: Yet Another NewsWatcher 2.2.0b7 Xref: biosci bionet.molbio.proteins:14297 bionet.molbio.methds-reagnts:75903 In article , i.mcfarlane@icrf.icnet.uk (Ian Mc) wrote: > Hi, > > Which form of glutathione do I use to elute my protein from > glutathione-agarose beads? The oxidised form of the reduced form??? > > Thanks, > > Ian Mc Also, be sure to pH the Glutathione solution before trying to elute. Below 8.0 you won't get much elution. Good luck, Zal From owner-proteins@net.bio.net Wed May 05 23:00:00 1999 Path: biosci!IP.OSGF.GE!dmn From: dmn@IP.OSGF.GE (David Managadze) Newsgroups: bionet.molbio.proteins Subject: Help me to purify AVIDIN from egg white... Date: 6 May 1999 08:59:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <3731C6F8.E5D48836@ip.osgf.ge> NNTP-Posting-Host: net.bio.net Hi, I need the method to purify AVIDIN from egg white (urgently, this is very significant for my Sc. work... ). I would be glad if you can give me some information about it... Sorry for my poor english :-) David M Newsgroups: bionet.molbio.proteins Subject: Re: Help me to purify AVIDIN from egg white... Date: Thu, 06 May 1999 18:46:35 -0600 Organization: The University of Texas at Austin, Austin, Texas Lines: 25 Message-ID: <373237CF.E0A2C4@mail.utexas.edu> References: <3731C6F8.E5D48836@ip.osgf.ge> Reply-To: lnd@mail.utexas.edu NNTP-Posting-Host: dhcp-71-140.botany.utexas.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.5 (Macintosh; I; PPC) X-Accept-Language: en David Managadze wrote: > Hi, > I need the method to purify AVIDIN from egg white (urgently, this is > very significant for my Sc. work... ). I would be glad if you can give > me some information about it... > > Sorry for my poor english :-) > > David M Newsgroups: bionet.molbio.proteins Subject: source for antibodies Date: Fri, 7 May 1999 10:34:23 -0700 Organization: University of Washington Lines: 21 Message-ID: NNTP-Posting-Host: dante40.u.washington.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Trace: nntp4.u.washington.edu 926098467 24756 (None) 140.142.17.37 X-Complaints-To: help@cac.washington.edu NNTP-Posting-User: qshang Hi, I'm looking for a commercial source for antibodies against plant cytochrome P450's. Any tips will be appreciated. Also, does anybody know of leaf-specific P450s? Thanks a lot, Tanya ----------------------------- Tanya Shang Department of Biochemistry Box 357350 University of Washington Seattle, WA 98195 qshang@u.washington.edu ----------------------------- From owner-proteins@net.bio.net Thu May 06 23:00:00 1999 From: Sebastian Bunka Newsgroups: bionet.molbio.proteins,bionet.molbio.methds-reagnts Subject: Re: GST purification Date: 7 May 1999 09:02:33 GMT Organization: University of Economics and Business Administration, Vienna, Austria Lines: 25 Sender: Sebastian Bunka Message-ID: <7gua79$5jb@cantine.wu-wien.ac.at> References: NNTP-Posting-Host: i112pc09.vu-wien.ac.at User-Agent: tin/pre-1.4-981002 ("Phobia") (UNIX) (Linux/2.2.5 (i586)) Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!newsfeed.tli.de!newsfeed.nacamar.de!newsfeed.arcor-ip.de!news-fra1.dfn.de!aconews.univie.ac.at!newsfeed.wu-wien.ac.at!not-for-mail Xref: biosci bionet.molbio.proteins:14300 bionet.molbio.methds-reagnts:75922 In bionet.molbio.methds-reagnts z wrote: > In article , > i.mcfarlane@icrf.icnet.uk (Ian Mc) wrote: >> Hi, >> >> Which form of glutathione do I use to elute my protein from >> glutathione-agarose beads? The oxidised form of the reduced form??? >> >> Thanks, >> >> Ian Mc The reduced form is the free tripeptide - this one works very fine for me (I use the one from sigma: G-4251 -- I'm not affiliated with sigma!). I have no idea wether the oxidized form (i.e. two tripeptides linked by the disulfide bond) works for elution. Sebastian -- Sebastian Bunka, Dr. med. vet Inst. Med. Chemistry, Vet. University Vienna Ph. +43-1-250 77 ext. 4208, Fax: ext. 4290 e-mail: Sebastian.Bunka@vu-wien.ac.at From owner-proteins@net.bio.net Thu May 06 23:00:00 1999 Path: biosci!HOTMAIL.COM!nshh303 From: nshh303@HOTMAIL.COM ("shanghai nie") Newsgroups: bionet.molbio.proteins Subject: Re:Help me to purify AVIDIN from egg white... Date: 7 May 1999 20:36:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <19990508033039.15973.qmail@hotmail.com> NNTP-Posting-Host: net.bio.net Hi David, you can use iminobiotin as ligand to purify Avidin, i did it about 8 years ago. Sorry for forgetting the related references. Sorry for my poor english :-) too nshh ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com From owner-proteins@net.bio.net Fri May 07 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!newsfeed.amsterdam.nl.net!sun4nl!surfnet.nl!barba.uci.kun.nl!not-for-mail From: "piet" Newsgroups: bionet.molbio.proteins Subject: Re: protein interactions/salt Date: Sat, 8 May 1999 15:27:24 +0200 Organization: Universitair Centrum Informatievoorziening, The Netherlands Lines: 32 Message-ID: <7h1e3t$8v6$1@barba.uci.kun.nl> References: <373102CA.6E28FAEF@scripps.edu> NNTP-Posting-Host: m38-031.fmw.kun.nl X-Newsreader: Microsoft Outlook Express 4.72.2106.4 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4 John Luz wrote in message <373102CA.6E28FAEF@scripps.edu>... >Hi Folks--I have overexpressed and purified two proteins. These two >intracellular proteins interact constitutively. This has been >demonstrated using whole cell lysates, immunoprecipitation, etc. I am >trying to confirm the interaction in vitro. > I incubated the two proteins for one hour on ice in 20 mM hepes pH 7.3/ >0.3 M KCl/ 10% glycerol. When applied to a gel filtration column the >two proteins eluted separately and not as a complex. One of my proteins >tends to aggregate in low ionic strength buffers, which is why I add KCl >to 0.3M. But I am concerned that the salt concentration is too high >for the complexes to form. Generally, speaking is this more salt than >your average protein/protein interaction can handle? Also I would >welcome any general comments regarding the experimental demonstration of >in vitro protein interactions. Thanks.--john Hi John, try in vitro crosslinking using DMS (Dimethylsuberimidate) or some other crosslinker. Next apply it on the same GPC column and study the elution pattern. As a control be sure to perform the same experiment for the proteins separately especially if you are afraid that one of them can form homomers. Hope this helps, Bas Kokke Dept. of Biochemistry, University of Nijmegen From owner-proteins@net.bio.net Sat May 08 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!dispose.news.demon.net!demon!newsfeed.icl.net!newsfeed.nacamar.de!rz.uni-karlsruhe.de!news.uni-stuttgart.de!news.ruhr-uni-bochum.de!not-for-mail From: Holger Neumann Newsgroups: bionet.molbio.proteins Subject: Re: source for antibodies Date: Sun, 09 May 1999 11:35:35 +0200 Organization: Ruhr-Universitaet Bochum, Rechenzentrum Message-ID: <373556E7.529053CD@ruhr-uni-bochum.de> References: NNTP-Posting-Host: dialppp-3-234.rz.ruhr-uni-bochum.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: sunu789.rz.ruhr-uni-bochum.de 926246752 10898 134.147.3.234 (9 May 1999 10:45:52 GMT) NNTP-Posting-Date: 9 May 1999 10:45:52 GMT X-Mailer: Mozilla 4.5 [de] (WinNT; I) X-Accept-Language: de Lines: 14 > Hi, > I'm looking for a commercial source for antibodies against plant > cytochrome P450's. Any tips will be appreciated. There is a nice page in the www: http://www.antibodyresource.com Hope this wil help. Holger From owner-proteins@net.bio.net Sun May 09 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!newspeer.monmouth.com!news.belnet.be!news-ge.switch.ch!news.grnet.gr!news.ntua.gr!not-for-mail From: pc Newsgroups: bionet.molbio.proteins Subject: Amino Acid No 21 Date: Mon, 10 May 1999 14:28:09 -0700 Organization: aua Lines: 7 Message-ID: <37374F68.95081512@auadec.aua.gr> Reply-To: pcostas@freemail.gr NNTP-Posting-Host: efa0pc82.aua.gr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-7 Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.05 [en] (Win95; I) please i need help with my work. Does anyone Know where to find information about the Amino Acid No 21 ? Any help will be very much appriciated. Thanks in advance Costas From owner-proteins@net.bio.net Sun May 09 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!dispose.news.demon.net!demon!newspeer.clara.net!news.clara.net!newsfeed.nacamar.de!fu-berlin.de!server1.netnews.ja.net!news.qub.ac.uk!not-for-mail From: Andrew Wallace Newsgroups: bionet.molbio.proteins Subject: Re: finger-shaped dialysis membrane Date: Mon, 10 May 1999 10:13:31 +0100 Organization: Queen's University Belfast Message-ID: <3736A339.2807@qub.ac.nospam.uk> References: <7g60hs$vge$1@news.doit.wisc.edu> Reply-To: a.wallace@qub.ac.nospam.uk NNTP-Posting-Host: awall.bc.qub.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; PPC) Lines: 25 Weiping, Sartorius used to make these devices, try them. Andrew Weiping Jiang wrote: > > Hi, there: > > I would like to try the finger-shaped dialysis membrane to handle small amount > of proteins. For instances, it is easy to change buffer, to concentrate > proteins, etc. I am wondering where I can purchase it. Could anyone there > give me some info? Thanks. > > Weiping -- ================================================================== Andrew Wallace, Ph.D. School of Biology and Biochemistry, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland (UK). Tel. : +44 (0)7074 226373 Fax : +44 (0)7074 426373 Email: a.wallace@qub.ac.nospam.uk (remove "nospam" to email me) WWW : http://www.qub.ac.uk/bb/wallace.html From owner-proteins@net.bio.net Sun May 09 23:00:00 1999 Path: biosci!agate!not-for-mail From: lhom@OCF.Berkeley.EDU (Louis Hom) Newsgroups: bionet.molbio.proteins Subject: Mannose-6-phosphate detection Date: 11 May 1999 01:32:54 GMT Organization: Univ. of California Berkeley Open Computing Facility Lines: 8 Message-ID: <7h81c6$b8n$1@agate.berkeley.edu> NNTP-Posting-Host: apocalypse.ocf.berkeley.edu I've got a protein that may or may not be a lysosomal protein, and I'm interested in trying to see if it contains mannose-6-phosphate. Can anyone suggest a method or a place to look at methods? -- ______________________________________________________________________________ Lou Hom >K'93 lhom@ocf.berkeley.edu http://www.ocf.berkeley.edu/~lhom/ From owner-proteins@net.bio.net Sun May 09 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!news-lond.gip.net!news.gsl.net!gip.net!newspeer.clara.net!news.clara.net!newsfeed.icl.net!newsfeed.nacamar.de!fu-berlin.de!server1.netnews.ja.net!news.qub.ac.uk!not-for-mail From: Andrew Wallace Newsgroups: bionet.molbio.proteins Subject: Re: finger-shaped dialysis membrane Date: Mon, 10 May 1999 10:14:27 +0100 Organization: Queen's University Belfast Lines: 25 Message-ID: <3736A371.3FB8@qub.ac.nospam.uk> References: <7g60hs$vge$1@news.doit.wisc.edu> Reply-To: a.wallace@qub.ac.nospam.uk NNTP-Posting-Host: awall.bc.qub.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; PPC) Weiping, Sartorius used to make these devices, try them. Andrew Weiping Jiang wrote: > > Hi, there: > > I would like to try the finger-shaped dialysis membrane to handle small amount > of proteins. For instances, it is easy to change buffer, to concentrate > proteins, etc. I am wondering where I can purchase it. Could anyone there > give me some info? Thanks. > > Weiping -- ================================================================== Andrew Wallace, Ph.D. School of Biology and Biochemistry, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland (UK). Tel. : +44 (0)7074 226373 Fax : +44 (0)7074 426373 Email: a.wallace@qub.ac.nospam.uk (remove "nospam" to email me) WWW : http://www.qub.ac.uk/bb/wallace.html From owner-proteins@net.bio.net Mon May 10 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!awabi.library.ucla.edu!134.139.1.31!csulb.edu!usenet From: "Jeffrey A. Cohlberg" Newsgroups: bionet.molbio.proteins Subject: Biochemistry lecturer position Date: Tue, 11 May 1999 14:24:37 -0700 Organization: Cal. State Univ. Long Beach Lines: 31 Message-ID: <3738A015.26FF@csulb.edu> NNTP-Posting-Host: 134.139.180.141 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Macintosh; I; PPC) Opening for a Full-Time Lecturer, 1999-2001 California State University, Long Beach Department of Chemistry and Biochemistry Duties: Lecturing in introductory and intermediate biochemistry courses and supervision of biochemistry laboratory courses. Opportunities may also be available for lecturing in graduate level biochemistry courses and supervision of biochemistry colloquia for senior undergraduate and MS students. Fall 1999 classes begin on August 30. Salary commensurate with qualifications and experience. Qualifications: Ph. D. in Chemistry or Biochemistry or a closely related field. Ability to communicate effectively with a diverse campus community. Prior successful teaching experience preferred. Required Documentation: Letter of application; resume; three letters of recommendation; official transcript from institution awarding highest degree. Position open until filled (or recruitment canceled). Review of applications to begin May 15. Applications and required documentation should be addressed to: Dr. N. M. Senozan, Chair, Department of Chemistry and Biochemistry, 1250 Bellflower Blvd, Long Beach, CA 90840-3903. For more information contact Dr. Nail Sezozan (nsenozan@csulb.edu) or Dr. Jeffrey Cohlberg (cohlberg@csulb.edu). Equal Opportunity/Affirmative Action/Title IX Employer From owner-proteins@net.bio.net Mon May 10 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!howland.erols.net!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: Willy Wriggers Newsgroups: bionet.molbio.proteins Subject: update: multi-res. docking w/ Situs 1.1 Date: Wed, 12 May 1999 00:17:44 GMT Organization: Deja.com - Share what you know. Learn what you don't. Lines: 34 Message-ID: <7hahb5$tfk$1@nnrp1.deja.com> NNTP-Posting-Host: 132.239.156.46 X-Article-Creation-Date: Wed May 12 00:17:44 1999 GMT X-Http-User-Agent: Mozilla/4.51 [en] (X11; I; Linux 2.2.6 i686) X-Http-Proxy: 1.0 x35.deja.com:80 (Squid/1.1.22) for client 132.239.156.46 Hi all, A new version (1.1) of the free single-molecule, multi-resolution docking package went online at URL http://chemcca10.ucsd.edu/~situs Some of the new features are: - A more powerful and more accurate clustering algorithm to correlate features within structural data sets. - Electron microscopy: support of ASCII, CCP4, SPIDER, and MRC file formats, map file export in CCP4 format for visualization e.g. with Swiss PDB Viewer - Small-angle X-ray scattering: Support for docking of crystal structures to bead models from SAXS refinements (Chacon et al., Biophys. J. 74:2760-2775, 1998) More info can be found online and in the current issue of J. Structural Biology (1999) 125:185-195, URL http://www.academicpress.com/jsb Cheers, Willy -- Willy Wriggers, Ph.D. -- URL http://chemcca10.ucsd.edu/~wriggers Tel: (619)534-2913. Fax: (619)534-7042. E-mail: wriggers@ucsd.edu --== Sent via Deja.com http://www.deja.com/ ==-- ---Share what you know. Learn what you don't.--- From owner-proteins@net.bio.net Mon May 10 23:00:00 1999 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!128.230.129.106!news.maxwell.syr.edu!dispose.news.demon.net!demon!ayres.ftech.net!news.ftech.net!newspeer.clara.net!news.clara.net!nntp.news.xara.net!xara.net!server5.netnews.ja.net!news.york.ac.uk!not-for-mail From: "Peter Ashton" Newsgroups: bionet.molbio.proteins Subject: Re: Amino Acid No 21 Date: Tue, 11 May 1999 13:08:02 +0100 Organization: The University of York, UK Sender: pda2@york.ac.uk Message-ID: <7h96gf$5g5$1@pump1.york.ac.uk> References: <37374F68.95081512@auadec.aua.gr> <7h8vk6$j1h$1@news.stack.serpukhov.su> NNTP-Posting-Host: biolpc294.york.ac.uk X-Trace: pump1.york.ac.uk 926424399 5637 144.32.84.128 (11 May 1999 12:06:39 GMT) X-Complaints-To: abuse@york.ac.uk NNTP-Posting-Date: 11 May 1999 12:06:39 GMT X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Lines: 20 I thought it was hydroxyproline :) Pete zdf wrote in message news:7h8vk6$j1h$1@news.stack.serpukhov.su... > Seleno-cystein > > pc ïèøåò â ñîîáùåíèè <37374F68.95081512@auadec.aua.gr> ... > >please i need help with my work. > >Does anyone Know where to find information about the Amino Acid No 21 ? > >Any help will be very much appriciated. > > > >Thanks in advance > >Costas > > > > From owner-proteins@net.bio.net Mon May 10 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!newsfeed.gamma.ru!Gamma.RU!demos!news.stack.serpukhov.su!not-for-mail From: "zdf" Newsgroups: bionet.molbio.proteins Subject: Re: Amino Acid No 21 Date: Tue, 11 May 1999 15:06:36 +0400 Organization: Stack Inc. Lines: 12 Message-ID: <7h8vk6$j1h$1@news.stack.serpukhov.su> References: <37374F68.95081512@auadec.aua.gr> NNTP-Posting-Host: filimonov.ipr.serpukhov.su X-Newsreader: Microsoft Outlook Express 4.72.3110.1 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3110.3 Seleno-cystein pc ïèøåò â ñîîáùåíèè <37374F68.95081512@auadec.aua.gr> ... >please i need help with my work. >Does anyone Know where to find information about the Amino Acid No 21 ? >Any help will be very much appriciated. > >Thanks in advance >Costas > From owner-proteins@net.bio.net Tue May 11 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!nntp.abs.net!attbtf!attbt1!ip.att.net!news.abbott.com!news.pprd.abbott.com!not-for-mail From: Owen McCall Newsgroups: bionet.molbio.proteins Subject: Re: Amino Acid No 21 Date: Wed, 12 May 1999 10:18:53 -0500 Organization: Abbott Laboratories Lines: 25 Message-ID: <37399BDD.46CC41F4@intronabbott.com> References: <37374F68.95081512@auadec.aua.gr> <7h8vk6$j1h$1@news.stack.serpukhov.su> <7h96gf$5g5$1@pump1.york.ac.uk> NNTP-Posting-Host: lc942596.dhcp.pprd.abbott.com Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Trace: fizban.pprd.abbott.com 926522329 3352 10.251.241.77 (12 May 1999 15:18:49 GMT) X-Complaints-To: usenet@fizban.pprd.abott.com NNTP-Posting-Date: 12 May 1999 15:18:49 GMT X-Mailer: Mozilla 4.05 [en] (Win95; I) You're behind the times! Aspartame, that's the ticket! Roney Graf wrote: > In article <7h96gf$5g5$1@pump1.york.ac.uk>, "Peter Ashton" > wrote: > > > > pc ïèøåò â ñîîáùåíèè <37374F68.95081512@auadec.aua.gr> ... > > > >please i need help with my work. > > > >Does anyone Know where to find information about the Amino Acid No 21 ? > > > >Any help will be very much appriciated. > > > zdf wrote in message > > news:7h8vk6$j1h$1@news.stack.serpukhov.su... > > > Seleno-cystein > > > I thought it was hydroxyproline :) > > Cystine (the S-S-dimer of cystEine) used to be listed as a separate > amino acid, way back then... > > roney From owner-proteins@net.bio.net Tue May 11 23:00:00 1999 Path: biosci!CENTRAL.MURDOCH.EDU.AU!wylie From: wylie@CENTRAL.MURDOCH.EDU.AU (Steve Wylie) Newsgroups: bionet.molbio.proteins Subject: GFP or GUS fusion proteins Date: 12 May 1999 19:32:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone know of a protein which, if expressed in cells as a fusion with GFP or GUS, would prevent GFP/GUS expression? Would a C-terminus or N-terminus fusion be the most effective? And if such a protein was available, could it be used to block expression of other proteins in fusions? Thanks Steve Wylie XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Stephen J. Wylie, PhD W.A State Agricultural Biotechnology Centre Murdoch University, W.A. 6150 Australia +61 8 9360 2920 (Phone) +61 8 9360 6303 (Fax) wylie@central.murdoch.edu.au http://wwwscience.murdoch.edu.au/centres/sabc/ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX From owner-proteins@net.bio.net Tue May 11 23:00:00 1999 Date: Wed, 12 May 1999 13:48:24 +0200 From: roney.graf@uni-konstanz.de (Roney Graf) Newsgroups: bionet.molbio.proteins Subject: Re: Amino Acid No 21 Message-ID: References: <37374F68.95081512@auadec.aua.gr> <7h8vk6$j1h$1@news.stack.serpukhov.su> <7h96gf$5g5$1@pump1.york.ac.uk> X-Newsreader: MT-NewsWatcher 2.4.4 NNTP-Posting-Host: 134.34.126.34 X-Trace: 12 May 1999 13:48:20 +0100, 134.34.126.34 Organization: University of Constance, Germany Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!feeder.qis.net.MISMATCH!feeder.qis.net!dispose.news.demon.net!demon!ayres.ftech.net!news.ftech.net!newsfeed.icl.net!newsfeed.nacamar.de!rz.uni-karlsruhe.de!news.uni-stuttgart.de!news.belwue.de!news.uni-konstanz.de!roney.graf Lines: 19 In article <7h96gf$5g5$1@pump1.york.ac.uk>, "Peter Ashton" wrote: > > pc ïèøåò â ñîîáùåíèè <37374F68.95081512@auadec.aua.gr> ... > > >please i need help with my work. > > >Does anyone Know where to find information about the Amino Acid No 21 ? > > >Any help will be very much appriciated. > zdf wrote in message > news:7h8vk6$j1h$1@news.stack.serpukhov.su... > > Seleno-cystein > I thought it was hydroxyproline :) Cystine (the S-S-dimer of cystEine) used to be listed as a separate amino acid, way back then... roney From owner-proteins@net.bio.net Tue May 11 23:00:00 1999 Path: biosci!CENTRAL.MURDOCH.EDU.AU!wylie From: wylie@CENTRAL.MURDOCH.EDU.AU (Steve Wylie) Newsgroups: bionet.molbio.proteins Subject: GFP Date: 12 May 1999 19:47:38 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone know of a protein which, if expressed in cells as a fusion with GFP or GUS, would prevent GFP/GUS expression? Would a C-terminus or N-terminus fusion be the most effective? And if such a protein was available, could it be used to block expression of other proteins in fusions? Thanks Steve Wylie XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Stephen J. Wylie, PhD W.A State Agricultural Biotechnology Centre Murdoch University, W.A. 6150 Australia +61 8 9360 2920 (Phone) +61 8 9360 6303 (Fax) wylie@central.murdoch.edu.au http://wwwscience.murdoch.edu.au/centres/sabc/ XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX From owner-proteins@net.bio.net Tue May 11 23:00:00 1999 Path: biosci!CENTRAL.MURDOCH.EDU.AU!wylie From: wylie@CENTRAL.MURDOCH.EDU.AU (Steve Wylie) Newsgroups: bionet.molbio.proteins Subject: (none) Date: 12 May 1999 22:31:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone know of a protein which, if expressed in cells as a fusion with GFP or GUS, would prevent GFP/GUS expression? Would a C-terminus or N-terminus fusion be the most effective if one wanted to prevent GFP expression? Thanks Steve Wylie From owner-proteins@net.bio.net Tue May 11 23:00:00 1999 Path: biosci!AOL.COM!DOCTORHIM From: DOCTORHIM@AOL.COM Newsgroups: bionet.molbio.proteins Subject: Re: deglycosylation of Sf9 expressed proteins Date: 12 May 1999 10:15:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <7647d776.246b0f83@aol.com> NNTP-Posting-Host: net.bio.net Check out the booklet that comes with this Deglycosylation Kit for a discussion of the current technology. http://www.prozyme.com/glycoenzymes/welcome.html You can download a PDF file with references. From owner-proteins@net.bio.net Tue May 11 23:00:00 1999 Message-ID: <3739ACA9.D23FBECB@biozentrum.uni-wuerzburg.de> Date: Wed, 12 May 1999 18:30:33 +0200 From: Matthias Dreyer X-Mailer: Mozilla 4.02 [en] (X11; I; IRIX 5.3 IP20) MIME-Version: 1.0 Newsgroups: bionet.molbio.proteins Subject: deglycosylation of Sf9 expressed proteins Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit NNTP-Posting-Host: wbzx04.biozentrum.uni-wuerzburg.de Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!dispose.news.demon.net!demon!colt.net!newspeer.clara.net!news.clara.net!newsfeed.nacamar.de!uni-erlangen.de!uni-wuerzburg.de!wbzx04.biozentrum.uni-wuerzburg.de Lines: 21 Hello All, I am trying to find a protocol that describes the deglycosylation of proteins expressed in Sf9, e.g. which glycosylases are recommended etc. Also, does anyone have information on the >kind< of glycosylation that Sf9 put on proteins? Thanks for any help, Matthias -- Matthias Dreyer Biozentrum Universität Würzburg Physiologische Chemie II Am Hubland 97074 Würzburg email: mad@biozentrum.uni-wuerzburg.de phone: (+49)-931-8884170/4103 fax: (+49)-931-8884113 From owner-proteins@net.bio.net Wed May 12 23:00:00 1999 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 13 May 1999 02:00:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905130900.CAA00804@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-proteins@net.bio.net Wed May 12 23:00:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!dispose.news.demon.net!demon!baron.netcom.net.uk!netcom.net.uk!server3.netnews.ja.net!news.icnet!mac017058.guys.icnet.uk!user From: i.mcfarlane@icrf.icnet.uk (Ian Mc) Newsgroups: bionet.molbio.proteins Subject: Re: deglycosylation of Sf9 expressed proteins Date: Thu, 13 May 1999 11:02:18 +0100 Organization: Fund Message-ID: References: <3739ACA9.D23FBECB@biozentrum.uni-wuerzburg.de> NNTP-Posting-Host: mac017058.guys.icnet.uk Lines: 32 Hi Matthias, PNGaseF and possibly EndoH are good places to start for removal of N-linked (asparagine) sugars. Try bionet.glycosci you may get a few more replies. Ian Mc In article <3739ACA9.D23FBECB@biozentrum.uni-wuerzburg.de>, Matthias Dreyer wrote: > Hello All, > I am trying to find a protocol that describes the deglycosylation of > proteins expressed in Sf9, e.g. which glycosylases are recommended etc. > Also, does anyone have information on the >kind< of glycosylation that > Sf9 put on proteins? > > Thanks for any help, > Matthias > > -- > Matthias Dreyer > Biozentrum Universität Würzburg > Physiologische Chemie II > Am Hubland > 97074 Würzburg > email: mad@biozentrum.uni-wuerzburg.de > phone: (+49)-931-8884170/4103 > fax: (+49)-931-8884113 From owner-proteins@net.bio.net Thu May 13 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!news.he.net!news.louisville.edu!not-for-mail From: Elas Klein Newsgroups: bionet.molbio.proteins Subject: Elastase Assay Date: Fri, 14 May 1999 10:21:32 -0400 Organization: University of Louisville Lines: 6 Message-ID: <373C316A.49456185@athena.louisville.edu> Reply-To: Elias.Klein@Louisville.edu NNTP-Posting-Host: eklein3.kdp-mdr.louisville.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: hermes.louisville.edu 926691694 17258 136.165.137.124 (14 May 1999 14:21:34 GMT) X-Complaints-To: usenet@hermes.louisville.edu NNTP-Posting-Date: 14 May 1999 14:21:34 GMT X-Mailer: Mozilla 4.51 [en] (Win95; I) X-Accept-Language: en Hi: Does anyone know of an ELISA kit available for h-elastase. EM Science in the US has a kit based on turbidimetry, but we don't have the instrument. Thanks for your help Elias.Klein@louisville.edu From owner-proteins@net.bio.net Thu May 13 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!newspeer1.nac.net!news.maxwell.syr.edu!newsfeed.tli.de!newsfeed.nacamar.de!rz.uni-karlsruhe.de!news.uni-stuttgart.de!news.belwue.de!newsserv.uni-tuebingen.de!not-for-mail From: Byung-Hoon Kim Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins Subject: Protein Cross-Linking with DSP Date: Fri, 14 May 1999 17:28:07 +0200 Organization: Zentrum fuer Datumverarbeitung Lines: 24 Message-ID: <373C4107.94758B68@student.uni-tuebingen.de> NNTP-Posting-Host: id.biol.biologie.uni-tuebingen.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: newsserv.zdv.uni-tuebingen.de 926695719 16434 134.2.88.164 (14 May 1999 15:28:39 GMT) X-Complaints-To: usenet@newsserv.uni-tuebingen.de NNTP-Posting-Date: 14 May 1999 15:28:39 GMT X-Mailer: Mozilla 4.06 [en] (Win95; I) Xref: biosci bionet.molbio.methds-reagnts:76080 bionet.molbio.proteins:14332 Hi all, Is anybody experienced in protein cross-linking with DSP (Dithiobis(succinimidyl propionate))? Because of it's hydrophobic nature, I couldn't make it soluble. I've done it as written in J. Mol. Biol. 104, 243 (1976) except that I made a stock solution directly in DMF or DMSO instead of in methylene chloride. I've made the 0.1M stock solution in DMF or DMSO and prior to cross-linking this was diluted 100 fold in PBS to yield 1mM solution for protein cross-linking applications.I could get more or less transparent solution but something flotes on the surface of the solution. When I centrifuge this, I can find a white pellet. Can anybody help me? Thanks in advance Byung-Hoon Kim (PhD student) Zentrum fuer MolekularBiologie der Pflanzen Dept. of General Genetics Univ. Tuebingen Germany From owner-proteins@net.bio.net Thu May 13 23:00:00 1999 Path: biosci!NS1.SUBMISSIONSERVICE.COM!sales From: sales@NS1.SUBMISSIONSERVICE.COM Newsgroups: bionet.molbio.proteins Subject: ADV: your web site Date: 14 May 1999 15:02:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 74 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905142202.PAA25011@net.bio.net> NNTP-Posting-Host: net.bio.net This email is being sent in compliance with all state and federal laws. You can remove your email address by returning this email with the word remove in the "SUBJECT FIELD" or by calling toll free 800-771-2003. Sender is Traffic Accelerator 5098 Foothills Blvd., #3136, Roseville, CA 95747 No cost to have email address removed. ================================================== IMMEDIATELY INCREASE YOUR SITES EXPOSURE Traffic Accelerator Will Submit Your Web Site To Over 900 Search Engines, Directories, & Indices For A Cost Of Only $ 14.95 (*) http://www.submissionservice.com =========== READ THIS TESTIMONIAL "The day after we received confirmation from you that you had submitted our web site to search engines, directories and indices we got a "Niagra" of hits! In fact, we doubled and almost tripled our "hits." And since then we have had a pattern of hits that has doubled the number of hits we averaged for the same period last year. For this little country inn, you have developed sales for us we never expected to get." =========== http://www.submissionservice.com For Just Pennies Each We Will Submit Your Web Site To Over 900 Of The Net's Hottest Search Engines, Directories & Indices. 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From owner-proteins@net.bio.net Thu May 13 23:00:00 1999 Newsgroups: bionet.molbio.proteins Subject: Re: Elastase Assay From: immune@intergate.bc.ca (immunechem) Reply-To: immune@intergate.bc.ca Organization: Internet Gateway X-Newsreader: WinVN 0.99.7 References: <373C316A.49456185@athena.louisville.edu> MIME-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII NNTP-Posting-Host: pm47s27.intergate.bc.ca Message-ID: <373c90b8@carrera.intergate.ca> Date: 14 May 1999 14:08:08 -0800 X-Trace: 14 May 1999 14:08:08 -0800, pm47s27.intergate.bc.ca Lines: 17 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!arclight.uoregon.edu!logbridge.uoregon.edu!newspeer.monmouth.com!news.vphos.net!carrera.intergate.ca!pm47s27.intergate.bc.ca An extremely sensitive color assay for activity of elastase was developed by Dr. Marquardt and Me. see article in Analytical biochemistry 268, 151-156, 1999. The method was patent pending. Sample of the assay kit could be available from Dr. Marquardt (204) 474-8188 In article <373C316A.49456185@athena.louisville.edu>, e0klei01@athena.louisville.edu says... > >Hi: Does anyone know of an ELISA kit available for h-elastase. EM >Science in the US has a kit based on turbidimetry, but we don't have the >instrument. Thanks for your help > >Elias.Klein@louisville.edu > From owner-proteins@net.bio.net Fri May 14 23:00:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!newsfeed.cwix.com!130.185.14.36!torn!kwon!watserv3.uwaterloo.ca!not-for-mail From: Michael Allen Newsgroups: bionet.molbio.proteins Subject: Kcat determination Date: Sat, 15 May 1999 10:06:09 -0400 Organization: University of Waterloo Lines: 13 Message-ID: <373D7F50.6B1D66D1@sciborg.uwaterloo.ca> NNTP-Posting-Host: moffatt5.uwaterloo.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.5 [en] (Win95; I) X-Accept-Language: en Hello all I apologize in advance for the naivete of this question. When you are determining the Kcat of an enzyme which is a homodimer in its active state, do you use the molecular mass of the monomer or the dimer? Thanks Mike Allen Graduate Student University of Waterloo Ontario, Canada From owner-proteins@net.bio.net Fri May 14 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!uchinews2!newsswitch.lcs.mit.edu!sunqbc.risq.qc.ca!cloudbreak.rs.itd.umich.edu!srvr1.engin.umich.edu!news.cc.ukans.edu!not-for-mail From: PGegen@UKans.nolospamare.edu (Dr. Peter Gegenheimer) Newsgroups: bionet.molbio.proteins Subject: Re: Amino Acid No 21 Date: 15 May 1999 16:45:15 GMT Organization: Univ. Kansas (BCMB) Lines: 31 Message-ID: <7opiGDf98QgB-pn2-OwwdflYhsCld@rnaworld.bio.ukans.edu> References: <37374F68.95081512@auadec.aua.gr> <7h8vk6$j1h$1@news.stack.serpukhov.su> <7h96gf$5g5$1@pump1.york.ac.uk> <37399BDD.46CC41F4@intronabbott.com> Reply-To: PGegen@UKans.nolospamare.edu NNTP-Posting-Host: rnaworld.bio.ukans.edu Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-2" Content-Transfer-Encoding: quoted-printable X-Newsreader: ProNews/2 Version 1.00 On Wed, 12 May 1999 15:18:53, Owen McCall wrote: > You're behind the times! Aspartame, that's the ticket! *Knock it off! There are non-experts reading this group who might not "get" the joke. (For those readers: Aspartame is a dipeptide, the c-methyl ester of Asp-Phe.) At present, only 21 amino acids are known to be incorporated into polypeptides during translation. These are the 20 "common" ones plus selenocyteine. The other amino acids found in proteins are generated by post-translational modification; these include structural residues like hydroxyproline and hypolysine(?), and regulatory residues like phospho-serine, tyrosine, threonine; and a few others (pteroylglutamate-?). There are numerous other amino acids, such as carnatine, which are not found in proteins but are used in other pathways. Check out your local biochem textbook for these. > > In article <7h96gf$5g5$1@pump1.york.ac.uk>, "Peter Ashton" > > wrote: > > > > >Does anyone Know where to find information about the Amino Acid No 21 ? > > > > >Any help will be very much appriciated. o----------------------------------------------------------------------o | Dr. Peter Gegenheimer | Vox: 785-864-3939 FAX: 785-864-5321 | | Department of | PGegen@UKans.nospam.edu | | Molecular Biosciences | http://rnaworld.bio.ukans.edu/ | | & Dept. Evol Biology | | | University of Kansas |"When you have excluded the impossible, | | 2045 Haworth Hall | whatever remains, however improbable, | | Lawrence KS 66045-2106 | must be the truth." S. Holmes | o_____________________________|________________________________________o From owner-proteins@net.bio.net Fri May 14 23:00:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!solaris.cc.vt.edu!news.vt.edu!news.cc.ukans.edu!not-for-mail From: PGegen@UKans.nolospamare.edu (Dr. Peter Gegenheimer) Newsgroups: bionet.molbio.proteins,bionet.biophysics Subject: Re: fluorescent adenine analogues Date: 15 May 1999 16:32:34 GMT Organization: Univ. Kansas (BCMB) Lines: 32 Message-ID: <7opiGDf98QgB-pn2-HuTddgdGWcqi@rnaworld.bio.ukans.edu> References: <373944A5.3FD0D339@fuerst.de> Reply-To: PGegen@UKans.nolospamare.edu NNTP-Posting-Host: rnaworld.bio.ukans.edu Mime-Version: 1.0 Content-Type: text/plain; charset="iso-8859-2" Content-Transfer-Encoding: quoted-printable X-Newsreader: ProNews/2 Version 1.00 Xref: biosci bionet.molbio.proteins:14336 bionet.biophysics:4992 On Wed, 12 May 1999 09:06:45, "Frank F=81rst" wrote: > Hi, > > I am looking for fluorescent derivatives of adenine. I want to try if > I can measure adenine binding to my protein with one of these. > > Unfortunately, I don't even know the names of common fluorescent > derivatives of adenine - but I know that there are some. > > Can anybody help me? > > Thanks in advance, Frank The oldest fluorescent analogue of adenine (& the only one I can think of) is N(6)-ethenoadenine. The other fluoresecent relatives I can think of are derivatives of AMP, such as TNP-AMP in which the trinitrophenyl group is attached to the 2'(3') hydroxyl. The ethenoadenine-containing nucleotides can be incorporated into poly(A). THere are also etheno-derivatives of NAD, etc. o----------------------------------------------------------------------o | Dr. Peter Gegenheimer | Vox: 785-864-3939 FAX: 785-864-5321 | | Department of | PGegen@UKans.nospam.edu | | Molecular Biosciences | http://rnaworld.bio.ukans.edu/ | | & Dept. Evol Biology | | | University of Kansas |"When you have excluded the impossible, | | 2045 Haworth Hall | whatever remains, however improbable, | | Lawrence KS 66045-2106 | must be the truth." S. Holmes | o_____________________________|________________________________________o From owner-proteins@net.bio.net Fri May 14 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!enews.sgi.com!newshub1.home.com!news.home.com!news.rdc1.bc.wave.home.com.POSTED!not-for-mail Reply-To: "Achim Recktenwald" From: "Achim Recktenwald" Newsgroups: bionet.molbio.proteins References: <373D7F50.6B1D66D1@sciborg.uwaterloo.ca> Subject: Re: Kcat determination Lines: 20 X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Message-ID: Date: Sun, 16 May 1999 03:15:26 GMT NNTP-Posting-Host: 24.64.220.105 X-Complaints-To: abuse@home.net X-Trace: news.rdc1.bc.wave.home.com 926824526 24.64.220.105 (Sat, 15 May 1999 20:15:26 PDT) NNTP-Posting-Date: Sat, 15 May 1999 20:15:26 PDT Organization: @Home Network Canada Michael Allen wrote in message news:373D7F50.6B1D66D1@sciborg.uwaterloo.ca... > Hello all > I apologize in advance for the naivete of this question. > When you are determining the Kcat of an enzyme which is a homodimer in > its active state, do you use the molecular mass of the monomer or the > dimer? This is not such a naive question. If the enzyme is not active as a monomer, I would use the molecular weight for the dimer. The best way is always to make sure, the documentation very clearly shows what you used for your calculations. Achim From owner-proteins@net.bio.net Sat May 15 23:00:00 1999 Path: biosci!RNA.BIO.MQ.EDU.AU!anouwens From: anouwens@RNA.BIO.MQ.EDU.AU ("Amanda Nouwens") Newsgroups: bionet.molbio.proteins Subject: Re: protein elution from SDS-PAGE gels Date: 16 May 1999 18:39:22 -0700 Organization: Dept. of Biological Sciences Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905170133.LAA01810@llymarch.bio.mq.edu.au> References: <373f0a30.230385031@news.fas.harvard.edu> Reply-To: anouwens@proteome.org.au NNTP-Posting-Host: net.bio.net I am currently working with membrane proteins, and the best method for elution after after digestion with trypsin is to add (~8 ul) 50% acetonitrile/0.5% TFA, sonicate for 10 minutes, and then load ~1ul onto the MALDI target plus matrix. Try washing the gel pieces in 2.5mM Tris-HCl/50% acetonitrile before digestion. I have no problem with extracting the peptides, even from membrane proteins with this method. If you need more info, let me know. Cheers, Amanda. > Hello, i've been attempting to do mass spectroscopy work with a > membrane protein, and in order to do so we must do an in-gel digestion > with trypsin, and then a subsequent elution into ammonium bicarbonate. > However, when we have tried this with protein stained with Coomassie > blue, we have had extremely low yields with the elution. So we > suspect that this is a result of the chemistry behind fixing the > protein in the gel, and so if anyone has any information on the > chemistry behind this, or any suggestions on improved methods of > elution from SDS-PAGE gels, it would be much appreciated. Also, I > should mention that as this is a membrane protein, and therefore > extrememly hydrophobic, aggregation may be causing a problem with > elution as well. Thank you. Also, I would appreciate it if any > responses could be emailed to me as well, as I am not able to > regularly check this newsgroup. > > -Dodzie Sogah > > sogah@fas.harvard.edu > From owner-proteins@net.bio.net Sat May 15 23:00:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!logbridge.uoregon.edu!news.fas.harvard.edu!not-for-mail From: sogah@fas.harvard.edu (Dodzie Sogah) Newsgroups: bionet.molbio.proteins Subject: protein elution from SDS-PAGE gels Date: Sun, 16 May 1999 18:17:34 GMT Organization: Harvard University, Cambridge, Massachusetts Lines: 18 Message-ID: <373f0a30.230385031@news.fas.harvard.edu> NNTP-Posting-Host: feigenb.student.harvard.edu X-Newsreader: Forte Free Agent 1.11/32.235 Hello, i've been attempting to do mass spectroscopy work with a membrane protein, and in order to do so we must do an in-gel digestion with trypsin, and then a subsequent elution into ammonium bicarbonate. However, when we have tried this with protein stained with Coomassie blue, we have had extremely low yields with the elution. So we suspect that this is a result of the chemistry behind fixing the protein in the gel, and so if anyone has any information on the chemistry behind this, or any suggestions on improved methods of elution from SDS-PAGE gels, it would be much appreciated. Also, I should mention that as this is a membrane protein, and therefore extrememly hydrophobic, aggregation may be causing a problem with elution as well. Thank you. Also, I would appreciate it if any responses could be emailed to me as well, as I am not able to regularly check this newsgroup. -Dodzie Sogah sogah@fas.harvard.edu From owner-proteins@net.bio.net Sun May 16 23:00:00 1999 Path: biosci!GERMANYNET.DE!100.334032 From: 100.334032@GERMANYNET.DE Newsgroups: bionet.molbio.proteins Subject: Where can I get MT-MMP proteins? Date: 17 May 1999 01:12:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905170806.KAA03390@bilbo.germany.net> NNTP-Posting-Host: net.bio.net Dear colleagues, I intend to become active in the field of matrix metalloproteinases (MMP ). For this purpose I urgently need some MMP, especially membrane-type MMP (MT1-MMP = MMP14, MT2-MMP = MMP15, MT3-MMP = MMP16 and MT4-MMP = MMP17). Furthermore I am strongly interested in antibodies against these enzymes. Unfortunately I can't find a commercial vendor for this special type of MMP. Is there anybody who can help me? Thank you very much! Gerno From owner-proteins@net.bio.net Sun May 16 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!newsfeed.tli.de!newsfeed01.btx.dtag.de!fu-berlin.de!141.80.29.26!not-for-mail From: "Will / Essers" Newsgroups: bionet.molbio.proteins Subject: Re: Where can I get MT-MMP proteins? Date: Mon, 17 May 1999 13:02:36 +0200 Organization: InViTek GmbH Lines: 58 Message-ID: <7hp0nr$sug$2@fu-berlin.de> References: <199905170806.KAA03390@bilbo.germany.net> NNTP-Posting-Host: 141.80.29.26 Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Access: 16 1370 X-Trace: fu-berlin.de 926942779 29648 (none) X-Newsreader: Microsoft Outlook Express 4.72.2106.4 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4 100.334032@GERMANYNET.DE <100.334032@GERMANYNET.DE> schrieb in Nachricht <199905170806.KAA03390@bilbo.germany.net>... > > >Dear colleagues, > >I intend to become active in the field of >matrix metalloproteinases (MMP ). For this purpose >I urgently need some MMP, especially membrane-type >MMP (MT1-MMP = MMP14, MT2-MMP = MMP15, MT3-MMP = >MMP16 and MT4-MMP = MMP17). Furthermore I am strongly >interested in antibodies against these enzymes. > >Unfortunately I can't find a commercial vendor for >this special type of MMP. Is there anybody who >can help me? Thank you very much! > > Gerno > > Hi Gerno, You can obtain MT1-MMP, MT2-MMP, MT3-MMP and MT4-MMP as purified recombinant proteases from InViTek in Germany. We also offer corresponding antibodies to these proteins. In addition you can get Gelatinase B (MMP 9) and Collagenase-3 (MMP 13) as purified proteins form human. Please send a note to invitek@mdc-berlin.de to get more detailed information. Yours sincerely, Lutz Essers _______________________________________ InViTek GmbH Tel.: ++49 (0)30 / 9489-3796 Fax: ++49 (0)30 /9489-3795 e-mail: invitek@mdc-berlin.de http://www.invitek.de _______________________________________ From owner-proteins@net.bio.net Sun May 16 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!feed1.news.rcn.net!rcn!newsfeed1.swip.net!swipnet!newsfeed2.funet.fi!130.231.240.8.MISMATCH!ousrvr3.oulu.fi!sun1.oulu.fi!pkursula From: Petri Kursula Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins Subject: Re: Protein Cross-Linking with DSP Date: Mon, 17 May 1999 12:19:20 +0300 Organization: University of Oulu Lines: 41 Message-ID: References: <373C4107.94758B68@student.uni-tuebingen.de> NNTP-Posting-Host: sun1.oulu.fi Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Trace: ousrvr3.oulu.fi 926932761 19283 130.231.240.11 (17 May 1999 09:19:21 GMT) X-Complaints-To: news@news.oulu.fi NNTP-Posting-Date: 17 May 1999 09:19:21 GMT To: Byung-Hoon Kim X-Sender: pkursula@sun1.oulu.fi In-Reply-To: <373C4107.94758B68@student.uni-tuebingen.de> Xref: biosci bionet.molbio.methds-reagnts:76119 bionet.molbio.proteins:14342 I have made a stock of 10 mM in DMSO,and then used this at 0.5 mM for the experiment. I have had no problem with the solubility. On Fri, 14 May 1999, Byung-Hoon Kim wrote: > Hi all, > > Is anybody experienced in protein cross-linking with DSP > (Dithiobis(succinimidyl propionate))? > > Because of it's hydrophobic nature, I couldn't make it soluble. > I've done it as written in J. Mol. Biol. 104, 243 (1976) except that I > made a stock solution directly in DMF or DMSO instead of in methylene > chloride. I've made the 0.1M stock solution in DMF or DMSO and prior to > cross-linking this was diluted 100 fold in PBS to yield 1mM solution for > protein cross-linking applications.I could get more or less transparent > solution but something flotes on the surface of the solution. When I > centrifuge this, I can find a white pellet. > > Can anybody help me? > Thanks in advance > > Byung-Hoon Kim (PhD student) > > Zentrum fuer MolekularBiologie der Pflanzen > Dept. of General Genetics > Univ. Tuebingen > Germany > > > ----------------------------------------------------------------------- Petri Kursula "I am somehow less interested in the weight and University of Oulu convolutions of Einstein's brain than in the near Petri.Kursula@oulu.fi certainty that people of equal talent have lived http://start.at/MAG and died in cotton fields and sweatshops." http://cc.oulu.fi/~pkursula -- Stephen Jay Gould ----------------------------------------------------------------------- From owner-proteins@net.bio.net Sun May 16 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!uchinews2!newsswitch.lcs.mit.edu!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: Kresten Newsgroups: bionet.molbio.proteins Subject: Re: Kcat determination Date: Mon, 17 May 1999 14:55:35 GMT Organization: Deja.com - Share what you know. Learn what you don't. Lines: 11 Message-ID: <7hpal6$hbc$1@nnrp1.deja.com> References: <373D7F50.6B1D66D1@sciborg.uwaterloo.ca> <7hpa8i$gtj$1@nnrp1.deja.com> NNTP-Posting-Host: 130.226.183.6 X-Article-Creation-Date: Mon May 17 14:55:35 1999 GMT X-Http-User-Agent: Mozilla/4.5 [en] (Win95; I) X-Http-Proxy: 1.0 www2.crc.dk:3128 (Squid/2.1.RELEASE), 1.0 x29.deja.com:80 (Squid/1.1.22) for client 130.226.182.217, 130.226.183.6 BTW I forgot to say that Kcat is usually called kcat. K's are eq. constants and k's rate constants (in this context anyway). Hope I am not stating the obvious. Kresten --== Sent via Deja.com http://www.deja.com/ ==-- ---Share what you know. Learn what you don't.--- From owner-proteins@net.bio.net Sun May 16 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!feed1.news.rcn.net!rcn!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: Kresten Newsgroups: bionet.molbio.proteins Subject: Re: Kcat determination Date: Mon, 17 May 1999 14:48:50 GMT Organization: Deja.com - Share what you know. Learn what you don't. Lines: 53 Message-ID: <7hpa8i$gtj$1@nnrp1.deja.com> References: <373D7F50.6B1D66D1@sciborg.uwaterloo.ca> NNTP-Posting-Host: 130.226.183.6 X-Article-Creation-Date: Mon May 17 14:48:50 1999 GMT X-Http-User-Agent: Mozilla/4.5 [en] (Win95; I) X-Http-Proxy: 1.0 www2.crc.dk:3128 (Squid/2.1.RELEASE), 1.0 x28.deja.com:80 (Squid/1.1.22) for client 130.226.182.217, 130.226.183.6 In article <373D7F50.6B1D66D1@sciborg.uwaterloo.ca>, Michael Allen wrote: > Hello all > I apologize in advance for the naivete of this question. > When you are determining the Kcat of an enzyme which is a homodimer in > its active state, do you use the molecular mass of the monomer or the > dimer? Depends on what you are doing. If the dimerisation equilibrium is: 2M = D where M is the monomer and D the dimer (and = here and in the rest does not mean equilibrium) and almost all of the protein is in D, then you can go ahead as if D were your enzyme E in the simple MM mech: E+S = ES -> E+P or whatever system you are working with. You must however be aware that if you solve for [D] using K_dimerisation you cannot use: [M]+2[D]=[enzyme]_total since there is also some Michaelis-complex present. What you really ought to do is to solve the equations of the system e.g. under steady-state conditions. If you are sure that only D and not M has activity the system could look something like: D + S = DS -> D+P II 2M (sorry about the drawing, it's an ASCII world) You can probably find the solution for this problem in an enz. kinetics textbook, but I would think it is faster to solve the equations rather than to go to the library. If you are not sure whether M also has activity you certainly have to do more experiments e.g. not only varying [S] but also [enzyme]_tot. I would guess you again could find info in an enzyme kinetics textbook and again recommend to do the algebra yourself. Hope this helps Kresten -- The address kresten@my-dejanews.com is for spambots only. Please mail me at LysLeuLeu@crc.dk , transforming the pre@-part into my initials. Kresten Lindorff Larsen, Dept. Yeast Genetics Carlsberg Laboratory, Denmark --== Sent via Deja.com http://www.deja.com/ ==-- ---Share what you know. Learn what you don't.--- From owner-proteins@net.bio.net Sun May 16 23:00:00 1999 Path: biosci!AMBER.BIOLOGY.GATECH.EDU!john From: john@AMBER.BIOLOGY.GATECH.EDU (John D. Besemer) Newsgroups: bionet.molbio.proteins Subject: UPDATE - Bioinformatics Conference in Atlanta Date: 17 May 1999 18:09:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 130 Sender: daemon@net.bio.net Distribution: world Message-ID: <9905180057.AA10828@exon.gatech.edu> NNTP-Posting-Host: net.bio.net SECOND GEORGIA TECH INTERNATIONAL CONFERENCE ON BIOINFORMATICS In silico BIOLOGY: SEQUENCE & STRUCTURE & FUNCION ATLANTA NOVEMBER 11 - 14, 1999 SPONSORS: National Institutes of Health US Department of Energy Alfred P. Sloan Foundation Georgia Tech College of Science Parker H. Petit Institute for Bioengineering & Bioscience SouthEastern Center for Applied Analysis SmithKline Beecham AGENDA: The conference agenda includes keynote lectures, plenary lectures, as well as poster sessions. The list of confirmed speakers is as follows: KEYNOTE SPEAKERS: Russell Doolittle University of California, San Diego, CA Walter Fitch University of California, Irvine, CA PLENARY SPEAKERS: David Baker University of Washington, Seattle, WA Pierre Baldi Net-ID, Pasadena, CA Steven Brenner Stanford University, Stanford, CA Chris Burge MIT, Cambridge, MA Antoine Danchin Institute Pasteur, Paris, France Sean Eddy Washington University School of Medicine, St. Louis, MO Patrick Forterre University Paris-Sud, Paris, France David Haussler University of California, Santa Cruz, CA Samuel Karlin Stanford University, Stanford, CA Jeffrey Lawrence University of Pittsburgh, Pittsburgh, PA Steven Henikoff Fred Hutchinson Cancer Research Center, Seattle, WA Christine Orengo University College, London, UK Burkhard Rost Columbia University, New York, NY Andrej Sali Rockefeller University, New-York, NY William Taylor National Institute for Medical Research, London, UK STEERING/PROGRAM COMMITTEE: Pierre Baldi Net-ID Mark Borodovsky, Co-chair Georgia Tech Soren Brunak Technical University of Denmark Chris Burge MIT Jim Fickett SmithKline Beecham Steven Henikoff Fred Hutchinson Cancer Research Center Eugene Koonin, Co-chair NCBI/NIH Andrej Sali Rockefeller University Chris Sander Millennium Pharmaceuticals Gary Stormo University of Colorado DEADLINES: FULL LENGTH MANUSCRIPT SUBMISSION: Special issue of "Bioinformatics" magazine will publish papers submitted by the conference participants presenting either talks or poster papers. Deadline for manuscript submission: June 18, 1999 POSTER SUBMISSION: Deadline for poster abstract submission: September 10, 1999 REGISTRATION: Early registration ends: October 1, 1999 CONFERENCE SCHEDULE: Registration opens at 6:00pm on Thursday, November 11 The program starts 8:00am Friday, November 12 and ends at noon Sunday, November 14. LOCATION: The conference will be held at the Renaissance Atlanta Hotel Downtown located near the center of 1996 Olympic development, close to the Fox Theatre & Georgia Tech. ORGANIZING COMMITTEE: Overall co-ordination Mark Borodovsky, Georgia Tech mark@amber.gatech.edu "Bioinformatics" magazine publication Chris Burge MIT cburge@mit.edu Poster sessions Scott Sammons Emory University sammons@bimcore.emory.edu Publicity John Besemer Georgia Tech john@amber.gatech.edu Registration and general events Michael Moryc Georgia Tech michael.moryc@conted.gatech.edu MORE INFORMATION To obtain more information on manuscript or poster submission & registration visit the WWW page: http://exon.biology.gatech.edu/conference or contact us by e-mail: register@conted.swann.gatech.edu Phone: (404) 894-2400 Fax: (404) 894-8925 ____________________________________ If you would like to be removed from this email list, please send a reply to john@amber.biology.gatech.edu with "REMOVE" in the subject field. From owner-proteins@net.bio.net Sun May 16 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!news.ucdavis.edu!dilbert.ucdavis.edu!not-for-mail From: szrathje@dilbert.ucdavis.edu (John Rathjen) Newsgroups: bionet.molbio.proteins Subject: Re: Amino Acid No 21 Date: 17 May 1999 18:42:01 GMT Organization: University of California, Davis Lines: 17 Message-ID: <7hpntp$q8j$1@mark.ucdavis.edu> References: <37374F68.95081512@auadec.aua.gr> <7h8vk6$j1h$1@news.stack.serpukhov.su> <7h96gf$5g5$1@pump1.york.ac.uk> <37399BDD.46CC41F4@intronabbott.com> <7opiGDf98QgB-pn2-OwwdflYhsCld@rnaworld.bio.ukans.edu> <374053BC.5AE780CE@mail.nih.gov> NNTP-Posting-Host: dilbert.ucdavis.edu X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Dieter Soll always used to state that there are ~130 amino acids in biology. I have no idea if these are modified aas in protein sequences, or independent molecules, or what...my 2c. John Jiro Takei (takeij@mail.nih.gov) wrote: : what about Aib (alpha-aminoisobutylic acid)? It is found in fungi. : alpha-hydrogen in alanine is replaced by a methyl group. : : Jiro : : Jiro Takei, Ph.D. : NCI, NIH : mailto:takeij@mail.nih.gov : -- From owner-proteins@net.bio.net Sun May 16 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!cpk-news-hub1.bbnplanet.com!washdc3-snh1.gtei.net!washdc3-snf1!news.gtei.net!icet.net.nih.gov!news From: Jiro Takei Newsgroups: bionet.molbio.proteins Subject: Re: Amino Acid No 21 Date: Mon, 17 May 1999 13:37:01 -0400 Organization: National Cancer Institute, NIH Lines: 9 Message-ID: <374053BC.5AE780CE@mail.nih.gov> References: <37374F68.95081512@auadec.aua.gr> <7h8vk6$j1h$1@news.stack.serpukhov.su> <7h96gf$5g5$1@pump1.york.ac.uk> <37399BDD.46CC41F4@intronabbott.com> <7opiGDf98QgB-pn2-OwwdflYhsCld@rnaworld.bio.ukans.edu> NNTP-Posting-Host: 137.187.208.14 Mime-Version: 1.0 Content-Type: text/plain; charset=iso-2022-jp Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.5 [ja] (Win95; I) X-Accept-Language: en,ja what about Aib (alpha-aminoisobutylic acid)? It is found in fungi. alpha-hydrogen in alanine is replaced by a methyl group. Jiro Jiro Takei, Ph.D. NCI, NIH mailto:takeij@mail.nih.gov From owner-proteins@net.bio.net Sun May 16 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!uchinews2!newsswitch.lcs.mit.edu!newspump.monmouth.com!newspeer.monmouth.com!news-was.dfn.de!newsjunkie.ans.net!news.louisville.edu!not-for-mail From: EKlein Newsgroups: bionet.molbio.proteins Subject: Pr A assays Date: Mon, 17 May 1999 12:00:42 -0400 Organization: University of Louisville Lines: 9 Message-ID: <37403D2A.2C26B676@athena.louisville.edu> Reply-To: Elias.Klein@Louisville.edu NNTP-Posting-Host: jslone2.kdp-baptist.louisville.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: hermes.louisville.edu 926956853 7129 136.165.136.53 (17 May 1999 16:00:53 GMT) X-Complaints-To: usenet@hermes.louisville.edu NNTP-Posting-Date: 17 May 1999 16:00:53 GMT X-Mailer: Mozilla 4.51 [en] (Win98; U) X-Accept-Language: en Could anyone recommend a commercial lab that can do Pr A assays in plasma and in saline elutes of immobilized Pr A columns? Need sensitivity in the ng/ ml range. Thanks for your help. -- Please note changed address is as follows: Elias.Klein@Louisville.Edu From owner-proteins@net.bio.net Sun May 16 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!news-feed.inet.tele.dk!bofh.vszbr.cz!newsfeed.tli.de!newsfeed.nacamar.de!fu-berlin.de!server1.netnews.ja.net!pegasus.csx.cam.ac.uk!hgmp.mrc.ac.uk!biosci From: chedwd@panther.Gsu.EDU ("Dabney W. Dixon") Newsgroups: bionet.molbio.proteins Subject: Monomer-dimer Keq Date: 17 May 1999 16:42:00 +0100 Organization: MRC Human Genome Mapping Project Resource Centre Lines: 38 Message-ID: NNTP-Posting-Host: mercury.hgmp.mrc.ac.uk Mime-Version: 1.0 Content-Type: MULTIPART/MIXED; BOUNDARY="-559023410-1804928587-926955624=:24792" X-Trace: niobium.hgmp.mrc.ac.uk 926955720 4993 193.62.192.80 (17 May 1999 15:42:00 GMT) X-Complaints-To: news@hgmp.mrc.ac.uk NNTP-Posting-Date: 17 May 1999 15:42:00 GMT Content-ID: X-Received: from panther.Gsu.EDU (IDENT:chedwd@panther.Gsu.EDU [131.96.1.18]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id QAA09491 for ; Mon, 17 May 1999 16:41:54 +0100 (BST) X-Received: from localhost (chedwd@localhost) by panther.Gsu.EDU (8.8.8/8.7.3) with SMTP id LAA10021; Mon, 17 May 1999 11:41:46 -0400 (EDT) X-Reply-To: "Dabney W. Dixon" X-To: proteins@hgmp.mrc.ac.uk X-cc: 3allen@sciborg.uwaterloo.ca This message is in MIME format. The first part should be readable text, while the remaining parts are likely unreadable without MIME-aware tools. Send mail to mime@docserver.cac.washington.edu for more info. ---559023410-1804928587-926955624=:24792 Content-Type: TEXT/PLAIN; CHARSET=US-ASCII Content-ID: The question being discussed is: In article <373D7F50.6B1D66D1@sciborg.uwaterloo.ca>, Michael Allen wrote: > Hello all > I apologize in advance for the naivete of this question. > When you are determining the Kcat of an enzyme which is a homodimer in > its active state, do you use the molecular mass of the monomer or the > dimer? Use the monomer. You can look at the equations in our recent paper Dixon, D.W. and Steullet, V. Dimerization of tetracationic porphyrins: Ionic strength dependence. J.Inorg.Biochem. 69:25-32, 1998 which is on small molecule dimerization but has the relevent algebra and references. If you want more on this type of thing, I think Gary Ackers papers on hemoglobin dimerization and tetramerization have clear descriptions. Dabney W. Dixon Department of Chemistry Georgia State University Atlanta, GA 30303 Phone: 404-651-3908 Fax: 404-651-1416 ddixon@gsu.edu ---559023410-1804928587-926955624=:24792-- --- From owner-proteins@net.bio.net Mon May 17 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!intgwpad.nntp.telstra.net!news1.optus.net.au!optus!news.uwa.edu.au!not-for-mail From: Graham McClorey Newsgroups: bionet.molbio.proteins Subject: Antibody to rat HNF-4 anybody? Date: 18 May 1999 09:55:49 GMT Organization: The University of Western Australia Lines: 12 Message-ID: <7hrdf5$fur$1@enyo.uwa.edu.au> NNTP-Posting-Host: tartarus.uwa.edu.au User-Agent: tin/pre-1.4-980226 (UNIX) (Linux/2.0.37 (i686)) Hi there! I am a research student at The University of Western Australia and am in desperate need of rabbit anti-rat HNF-4. I have not been able to locate this antibody through regular channels ie catalogues so i was wondering if anyone would be able to help me in locating it. Any help would be greatly appreciated. Thanks Graham Mc Clorey - Department of Biochemistry -- From owner-proteins@net.bio.net Mon May 17 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!newshub.northeast.verio.net!rutgers!news-relay.ncren.net!news.wfu.edu!news From: Steve Miles Newsgroups: bionet.molbio.proteins Subject: Re: protein elution from SDS-PAGE gels Date: Tue, 18 May 1999 11:17:56 -0400 Organization: Wake Forest University School of Medicine Lines: 37 Message-ID: <374184A4.4EB52830@wfubmc.edu> References: <373f0a30.230385031@news.fas.harvard.edu> NNTP-Posting-Host: 152.11.196.24 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.5 [en]C-CCK-MCD (WinNT; U) X-Accept-Language: en Hi! We work with an extremely hydrophobic membrane protein complex (signal peptidase) and had little results with coomassie stained in-gel digests. In order to get some good MALDI peptide maps I switched to Biorad's "zinc-stain" which is a negative stain. This worked pretty good, we found almost all of the peptides except for the membrane spans. Essentially you follow the coomassie protocol, but zinc stain the gel and wash the gel pieces with the zinc destaining solution provided with the kit. Then do the digestions. Good Luck, Steve Miles smiles@wfubmc.edu Dodzie Sogah wrote: > Hello, i've been attempting to do mass spectroscopy work with a > membrane protein, and in order to do so we must do an in-gel digestion > with trypsin, and then a subsequent elution into ammonium bicarbonate. > However, when we have tried this with protein stained with Coomassie > blue, we have had extremely low yields with the elution. So we > suspect that this is a result of the chemistry behind fixing the > protein in the gel, and so if anyone has any information on the > chemistry behind this, or any suggestions on improved methods of > elution from SDS-PAGE gels, it would be much appreciated. Also, I > should mention that as this is a membrane protein, and therefore > extrememly hydrophobic, aggregation may be causing a problem with > elution as well. Thank you. Also, I would appreciate it if any > responses could be emailed to me as well, as I am not able to > regularly check this newsgroup. > > -Dodzie Sogah > > sogah@fas.harvard.edu From owner-proteins@net.bio.net Mon May 17 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!intgwpad.nntp.telstra.net!news1.optus.net.au!optus!news.usyd.edu.au!sunb.ocs.mq.edu.au!not-for-mail From: Mark Molloy Newsgroups: bionet.molbio.proteins Subject: Re: protein elution from SDS-PAGE gels Date: Wed, 19 May 1999 16:30:39 +1000 Organization: Macquarie University, NSW, Australia Lines: 70 Message-ID: <37425A8E.3B5D7B71@rna.bio.mq.edu.au> References: <373f0a30.230385031@news.fas.harvard.edu> <199905170133.LAA01810@llymarch.bio.mq.edu.au> NNTP-Posting-Host: apaf2.bio.mq.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.5 [en] (Win95; I) X-Accept-Language: en Another factor to take into account is the Coomassie staining protocol. If you are using a TCA fixative method then its time to change. A sensitive method that allows protein recovery is 17% ammonium sulfate 34% methanol 3% phosphoric acid 0.1% Coomassie G250. Dissolve the salt with the MeOH/water mix before adding the acid and stain. Make fresh each time and stain O/N. Destain with 1% acetic acid and storage the gel in this solution. Then try the procedure described below Good luck. Mark Amanda Nouwens wrote: > I am currently working with membrane proteins, and the best method for elution after > after digestion with trypsin is to add (~8 ul) 50% acetonitrile/0.5% TFA, > sonicate for 10 minutes, and then load ~1ul onto the MALDI target plus matrix. > > Try washing the gel pieces in 2.5mM Tris-HCl/50% acetonitrile before digestion. > > I have no problem with extracting the peptides, even from membrane proteins > with this method. > > If you need more info, let me know. > Cheers, > > Amanda. > > > Hello, i've been attempting to do mass spectroscopy work with a > > membrane protein, and in order to do so we must do an in-gel digestion > > with trypsin, and then a subsequent elution into ammonium bicarbonate. > > However, when we have tried this with protein stained with Coomassie > > blue, we have had extremely low yields with the elution. So we > > suspect that this is a result of the chemistry behind fixing the > > protein in the gel, and so if anyone has any information on the > > chemistry behind this, or any suggestions on improved methods of > > elution from SDS-PAGE gels, it would be much appreciated. Also, I > > should mention that as this is a membrane protein, and therefore > > extrememly hydrophobic, aggregation may be causing a problem with > > elution as well. Thank you. Also, I would appreciate it if any > > responses could be emailed to me as well, as I am not able to > > regularly check this newsgroup. > > > > -Dodzie Sogah > > > > sogah@fas.harvard.edu > > -- N.B. NEW CONTACT DETAILS FROM 1.4.99 ******************************************** Mark Molloy Australian Proteome Analysis Facility (APAF) School of Biological Sciences Macquarie University Sydney, AUSTRALIA, 2109 Tel: +61 2 9850 6267 Fax: +61 2 9850 8174 http://www.proteome.org.au ******************************************** From owner-proteins@net.bio.net Mon May 17 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!howland.erols.net!news.maxwell.syr.edu!nntp.news.xara.net!xara.net!server6.netnews.ja.net!dundee.ac.uk!mfwhite From: mfwhite@bad.dundee.ac.uk (Malcolm White) Newsgroups: bionet.molbio.proteins Subject: Method of DNA binding protein detection after gel electrophoresis? Date: Tue, 18 May 1999 16:57:55 +0100 Organization: University of Dundee Lines: 18 Message-ID: NNTP-Posting-Host: mfwg3.bioch.dundee.ac.uk X-Newsreader: MT-NewsWatcher 2.4.1 I'm looking for a method to identify a DNA-binding protein in a protein mixture after separation by acrylamide gel electrophoresis. I have not had much success with a literature search, or with the various protocols books I can lay my hands on, so maybe someone out there can help... I'll be using a radioactive DNA probe to look for specific protein binding. Various possibilities for this experiment spring to mind: a) Native versus denaturing gel electrophoresis. b) 'In-gel' binding versus binding on a membrane surface after blotting. Can anyone point me in the right direction? Thanks, Malcolm White From owner-proteins@net.bio.net Mon May 17 23:00:00 1999 Path: biosci!CIENCIAS.CIENCIAS.UNAL.EDU.CO!bioquim From: bioquim@CIENCIAS.CIENCIAS.UNAL.EDU.CO (Gerardo Perez Quimica UNALCOL) Newsgroups: bionet.molbio.proteins Subject: Remove Date: 18 May 1999 06:40:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 80 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <199905142202.PAA25011@net.bio.net> NNTP-Posting-Host: net.bio.net On 14 May 1999 sales@ns1.submissionservice.com wrote: > This email is being sent in compliance with all > state and federal laws. You can remove your email > address by returning this email with the word > remove in the "SUBJECT FIELD" or by calling toll > free 800-771-2003. Sender is Traffic Accelerator > 5098 Foothills Blvd., #3136, Roseville, CA 95747 > No cost to have email address removed. > ================================================== > > > IMMEDIATELY INCREASE YOUR SITES EXPOSURE > > Traffic Accelerator Will Submit Your > > Web Site To Over 900 Search Engines, > > Directories, & Indices > > For A Cost Of Only $ 14.95 (*) > > http://www.submissionservice.com > > =========== > > READ THIS TESTIMONIAL > > "The day after we received confirmation from you that > you had submitted our web site to search engines, > directories and indices we got a "Niagra" of hits! > In fact, we doubled and almost tripled our "hits." > > And since then we have had a pattern of hits that has > doubled the number of hits we averaged for the same > period last year. > > For this little country inn, you have developed sales > for us we never expected to get." > > =========== > > http://www.submissionservice.com > > For Just Pennies Each We Will Submit Your > Web Site To Over 900 Of The Net's Hottest Search > Engines, Directories & Indices. > > If your site isn't listed in the Search Engines, > how can people find you to buy your products > or services? > > See why thousands and thousands of businesses > world wide both large and small have come to > us to utilize our services. Hotels, Motels, > On-Line Stores, Travel Agents, Colleges, > Universities, Governments, Fortune 500 companies, > Movie Studios, Chambers Of Commerce and many, > many more. Shouldn't you visit us today? > > http://www.submissionservice.com > > > (*) We submit your site MONTHLY to over 900 > search engines, directories and indices for a > total cost of just $14.95 per month. Minimum 12 > month term required. > > > > > > > > > > From owner-proteins@net.bio.net Tue May 18 23:00:00 1999 From: "RISHIKESH P. BHALERAO" Newsgroups: bionet.molbio.proteins Subject: Two post-doc positions Date: Wed, 19 May 1999 13:30:28 +0000 Organization: Swedish University of Agricultural Sciences Lines: 56 Message-ID: <3742BCEF.285E92A4@genfys.slu.se> NNTP-Posting-Host: d170.genfys.slu.se Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.04 (Macintosh; I; PPC) Path: biosci!agate!newsfeed.berkeley.edu!newsfeed1.swip.net!swipnet!news-peer-europe.sprintlink.net!news.sprintlink.net!newsfeed.sunet.se!news01.sunet.se!news99.sunet.se!newsfeed.uu.se!populus.slu.se!newsmgr Two Post-doctoral fellowships at the Dept of Forest Genetics and Plant Physiology in Umea, Sweden: We have two post-doc positions in our lab to work on the following projects: (I) The first position is in the area of cell cycle regulation during wood formation in Poplar. We have cloned cDNAs for several key cell cycle regulators from Poplar and are in the process of generating transgenic plants with modified levels of these cell cycle regulators in specific cell types in the wood forming tissues and assess the effect on wood formation in transgenic trees. (II) The second position is in the area of control of vascular development by IAA. We have cloned several genes rapidly upregulated by IAA and showing distinct tissue and cell type expression pattern in wood forming tissues of Poplar. The project focuses on elucidating the role of these IAA regulated genes in vascular development in Poplar and Arabidopsis. We are interested in employing highly motivated scientists with background in molecular biology and protein chemistry to work with us on the two projects. The projects involve collaboration with a team of scientists investigating wood formation using a variety of approaches involving analytical chemistry, microscopy and physiology. The work will involve construction and analysis of transgenic plants, cloning, expression analysis, in-situ hybridisation and analysis of protein phosphorylation. The positions are for 1+1 year starting as soon as possible for the applicant. The Dept of Forest Genetics and Plant Physiology employs over 70 scientists and students. We have sophisticated facilities for growth regulator measurements, confocal microscopy and several green houses. The city of Umea is a pleasent university town with excellent facilities for extracuricular activities with swimming pools, tennis and squash courts. In addition to the forestry department, the University of Umea has outstanding departments performing research in microbiology, animal and plant development. Interested individuals should send c.v. with name and addresses (fax and/or email) of three referees to: Dr. Rishikesh P. Bhalerao at the address below: Department of Forest Genetics and Plant Physiology The Swedish University of Agricultural Sciences S-901 83 Umea Sweden Tel:+46-90-7866282 Fax:+46-90-7865901 Email:rishi.bhalerao@genfys.slu.se From owner-proteins@net.bio.net Tue May 18 23:00:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!dispose.news.demon.net!demon!newsfeed.icl.net!newsfeed.nacamar.de!fu-berlin.de!news.ruhr-uni-bochum.de!not-for-mail From: Reinhard Newsgroups: bionet.molbio.proteins Subject: biotinylated NAD Date: Wed, 19 May 1999 16:28:52 +0200 Organization: Ruhr-Universitaet Bochum, Rechenzentrum Message-ID: <3742CAA4.2D65385D@hotmail.com> NNTP-Posting-Host: samson.microbiol.ruhr-uni-bochum.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: sunu789.rz.ruhr-uni-bochum.de 927124017 27093 134.147.129.6 (19 May 1999 14:26:57 GMT) NNTP-Posting-Date: 19 May 1999 14:26:57 GMT X-Mailer: Mozilla 4.51 [en] (Win95; I) X-Accept-Language: en,de-DE Lines: 11 Hi, I`m looking for a commercial or noncommercial source for biotinylated NAD. I want to us it as a substrat instead of 32P-labeled NAD. Another point is the possibility to purify the biotinylated target. Thank`s for any help. Reinhard Depping From owner-proteins@net.bio.net Thu May 20 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!news.maxwell.syr.edu!nntp.news.xara.net!xara.net!server6.netnews.ja.net!server4.netnews.ja.net!server2.netnews.ja.net!pegasus.csx.cam.ac.uk!not-for-mail From: "Dave Bolton" Newsgroups: bionet.molbio.proteins Subject: Re: 5/6 stranded barrels? Date: 21 May 1999 12:03:56 GMT Organization: University of Cambridge, England Lines: 18 Message-ID: <01bea381$67ec4a40$9f246f83@mole.bio.cam.ac.uk> References: <3745180B.41C6@wright.edu> NNTP-Posting-Host: ceylon.bioc.cam.ac.uk X-Newsreader: Microsoft Internet News 4.70.1155 Hi Deacon The best place to look is on the CATH web site. This breaks down protein structures into types. Select Class, beta, scroll down the architecture and select barrel. The number of strands is given next to it. The cath web site off the top of my head is http://www.ucl.ac.uk/bsm/CATH/ Heather Deacon Sweeney wrote in article <3745180B.41C6@wright.edu>... > Does anyone know of any families of five or six membered beta barrels? > I've found two... OB and SH3... are there any others? > > Deacon Sweeney > From owner-proteins@net.bio.net Thu May 20 23:00:00 1999 Path: biosci!agate!newsfeed.berkeley.edu!news-peer1.sprintlink.net!news-backup-west.sprintlink.net!news.sprintlink.net!cyclone.swbell.net!typhoon-sf.snfc21.pbi.net.POSTED!not-for-mail Message-ID: <37457ED1.796D4377@going-going-sold.com> From: Mark Atlas Organization: Internet Auctioneers Intl X-Mailer: Mozilla 4.51 [en] (Win98; I) X-Accept-Language: en MIME-Version: 1.0 Newsgroups: bionet.molbio.proteins Subject: Internet auction and marketplace for used lab equipment Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 241 Date: Fri, 21 May 1999 08:42:09 -0700 NNTP-Posting-Host: 207.214.111.210 X-Complaints-To: abuse@pacbell.net X-Trace: typhoon-sf.snfc21.pbi.net 927301456 207.214.111.210 (Fri, 21 May 1999 08:44:16 PDT) NNTP-Posting-Date: Fri, 21 May 1999 08:44:16 PDT Internet auction and marketplace for used lab equipment Please visit http://www.going-going-sold.com for the latest up to date auction listings. If the equipment you seek is not located on the auction block, you can also visit, http://www.going-going-sold.com/GGS/ggshome.nsf/LocFrameL?openform to place a request for competitive bids from all dealers, labs and lessors. The equipment bids will be sent back and you can choose from all of the equipment available.Over 140 requests for bids through the locator service have been submitted to Going,Going...Sold! We are currently ramping up our abilities to process all of the requests. Why spend your time shopping, we can do it for you better/faster and with our in lab evaluation of all purchases Recent price changes on the auction Saturn 1 GCMS lowered to $15,000. Includes 3400 and fully functional ion trap based GCMS form Varian. This is priced to sell. Biocad $22,000 Other specials not posted on this site: Fisons Kevex XRF, 3-5 yrs old, selling 50K Biomek 2000 1994(approximate)120K new selling 55K ABI 494 over 100K new selling 65K Excellent condition If your lab has a number of small or large items which you wish to sell, we can now provide an easy to use software to allow you to create ads offline and email the software back to us.Please reply with a request for a copy of our new surplus management software. ***************************************************** - Recent Arrivals - Opening Prices ***************************************************** 1. Varian SpectraAA 600 Flame $9,750.00 ------------------------------------------------------------ 2. Varian SpectrAA 600Z furnace $11,500.00 ------------------------------------------------------------ 3. Oil Immersion Microscope $150.00 ------------------------------------------------------------ 4. 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Hand Held Refractometers $245.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/19/99 Opening Prices ***************************************************** 1. Beckman DU-7 $3,500.00 ------------------------------------------------------------ 2. Beckman Gamma Counter $2,750.00 ------------------------------------------------------------ 3. Waters 680 Automated Gradient Controller for HPLC $1,460.00 ------------------------------------------------------------ 4. Fluoresence Microscope $4,800.00 ------------------------------------------------------------ 5. Superconducting NMR spectrometer $9,500.00 ------------------------------------------------------------ 6. Ultra-Low Temperature Freezer (-80c) 20.5 cu ft $2,400.00 ------------------------------------------------------------ 7. Waters Gradient HPLC $16,500.00 ------------------------------------------------------------ 8. Zymark SPE System $14,000.00 ------------------------------------------------------------ 9. ThermoSep HPLC System $14,500.00 ------------------------------------------------------------ 10. Quaternary HPLC w/ Data System $20,500.00 ------------------------------------------------------------ 11. Fisons MD-800 GCMS $27,800.00 ------------------------------------------------------------ 12. Waters PDA system with Millennium $25,900.00 ------------------------------------------------------------ ***************************************************** Sale Closing : 5/21/99 Opening Prices ***************************************************** 1. Leica Microscope $1,500.00 ------------------------------------------------------------ 2. Perkin Elmer AAS2100 Flame AA $12,000.00 ------------------------------------------------------------ 3. Lightweight sterilizer $3,600.00 ------------------------------------------------------------ 4. Varian GC-3400 $2,000.00 ------------------------------------------------------------ 5. Phase contrast Microscope w/camera $4,750.00 ------------------------------------------------------------ 6. 20 cubic foot Refridgerator $1,350.00 ------------------------------------------------------------ ***************************************************** (c) 1997, Internet Auctioneers International From owner-proteins@net.bio.net Thu May 20 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!newsfeed.cwix.com!192.232.20.2!malgudi.oar.net!hyperion.wright.edu!news.wright.edu!not-for-mail From: Deacon Sweeney Newsgroups: bionet.molbio.proteins Subject: 5/6 stranded barrels? Date: Fri, 21 May 1999 04:23:39 -0400 Organization: Wright State University Lines: 4 Message-ID: <3745180B.41C6@wright.edu> NNTP-Posting-Host: linus.bmb.wright.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01SGoldC-SGI (X11; I; IRIX64 6.4 IP30) Does anyone know of any families of five or six membered beta barrels? I've found two... OB and SH3... are there any others? Deacon Sweeney From owner-proteins@net.bio.net Thu May 20 23:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!news.he.net!news.pagesat.net!news.itis.com!news.doit.wisc.edu!default From: klenchin@facstaff.REMOVE_TO_REPLY.wisc.edu (Dima Klenchin) Newsgroups: bionet.molbio.proteins Subject: Re: Pierce vs. Novagen 6XHis column-B-Per Date: Sat, 22 May 1999 00:32:35 GMT Organization: UW-Madison Lines: 11 Message-ID: <7i4tu3$e1q$3@news.doit.wisc.edu> References: NNTP-Posting-Host: martinlab.biochem.wisc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=KOI8-R Content-Transfer-Encoding: 8bit X-Newsreader: News Xpress 2.01 X-No-Archive: yes :I am trying to determine which 6XHis columns to use. I also want to try :the B-Per reagent for fusion protein purification. Does anyone have :exprience with either of these columns and especially with the use of the :B-Per reagent with these columns. Thanks, Mary : The reagent is probably just buffered desoxycholate... Whatever it is, it can be used with any column from any supplier - most of them use the same NTA-Sepharose anyway. - Dima From owner-proteins@net.bio.net Thu May 20 23:00:00 1999 Path: biosci!kfunigraz.ac.at!andreas.kungl From: andreas.kungl@kfunigraz.ac.at (andreas kungl) Newsgroups: bionet.molbio.proteins Subject: 3rd International Conference on Molecular Structural Biology Date: 21 May 1999 07:41:32 -0700 Organization: Karl-Franzens-Universitaet Graz Lines: 326 Sender: daemon@net.bio.net Distribution: world Message-ID: <37456E89.119C1B97@kfunigraz.ac.at> NNTP-Posting-Host: net.bio.net The Biochemistry Subgroup of the Austrian Chemical Society is pleased to announce the Third International Conference on Molecular Structural Biology which will take place in Vienna, Austria from September 8-12, 1999 FOR DETAILED INFORMATION, PLEASE SEE BELOW OR VISIT OUR HOMEPAGE AT http://www.kfunigraz.ac.at/ipcwww/icmsb99 ************************************************************************ Introduction to the ICMSB99 The First and the Second International Conference on Molecular Structural Biology (ICMSB) took place in Vienna in September 1995 and 1997. Both conferences were very well received by all who took part, including over 250 participants from more than 20 countries worldwide. The organisers are pleased to announce the Third ICMSB, which will take place from 8th to 12th September 1999, and which will, like the previous conferences, feature internationally renowned speakers. The ICMSB99 will focus on topics covering a number of the most exciting areas in the field, with the aim of bringing together specialised scientists from different areas. It will be opened by one of the most outstanding scientists in the field of structural biology, Robert Huber, and the following four days of sessions will include Folding and Function, Novel Structures, Advances in Microscopic Methods, Structural Molecular Biology, Structure-Based Drug Design, and Prediction and Simulation. ************************************************************************ Vienna - An Attractive Conference Location The ICMSB99 will be located at the Federal Chancellery in Vienna. The city is a particularly attractive location for a conference, with its combination of historical buildings, green parks, and modern architecture. Some of the most famous city sights include Schönbrunn Palace and the Hofburg, former homes of the Habsburgs, St. Stephans Cathedral, and the colourful Hundertwasser House. Also unique to Vienna is the Prater park, with its endless green avenues and its funfair, featuring the ´´Riesenrad´´ (ferris wheel). Of course, no trip to Vienna would be complete without a visit to one of the many traditional Viennese cafés, for a piece of the famous Sachertorte. An ´´Oldtimer-Tram´´tour around the Ringstraße will take participants past many of the finest sights. ************************************************************************ Organising Committee A. Kungl, P. Andrew, A. Binder, S. Kristl, A. Schilk ************************************************************************ Scientific Committee C. Kratky, M. Sippl, A. Kungl, P. Andrew ************************************************************************ In Cooperation With Austrian Academy of Sciences Austrian Federal Chancellery ************************************************************************ Sponsored By Austrian Airlines Novartis Forschungsinstitut ************************************************************************ Scientific Programme The six sessions of the conference cover a wide range of topics and experimental methods, which will be presented in the form of plenary lectures, selected short oral communications, and posters. Wednesday 8th: Registration from 2 p.m. onwards Evening: Honorary Lecture (followed by a welcome drink and snack) Robert Huber (Max-Planck-Institute, Martinsried): Diversity and Conservation in Proteolytic Enzymes and their Inhibitors Thursday 9th: Morning Session: Advances in Microscopic Methods Werner Kühlbrandt (Max-Planck-Institute, Frankfurt): Two Conformations of Membrane Ion Pumps Hansgeorg Schindler (Linz University): Single Molecule Microscopy Methods for Structural Biology Helen Hansma (University of California, Santa Barbara): Probing Biomaterials with the Atomic Force Microscope Afternoon Session: Folding and Function Alan Fersht (Cambridge University): Minichaperones: Practical and Mechanistic Tools Thomas Kiefhaber (Biozentrum Basel): Speed Limit for Protein Folding Peter Wright (Scripps Institute): Structure and Dynamics of Unfolded Proteins and Protein Folding Intermediates Friday 10th: Morning Session: Novel Structures Michael Rossmann (Purdue University): The Assembly of Viruses Examined by Combining Crystallography and Cryo-electron Microscopy Don Wiley (Harvard University): Structural Studies of Viral Entry Mechanisms in Influenza, HIV-1 and bola Viruses Kurt Wüthrich (ETH Zürich): TROSY and BSE - Recent Progress with NMR in Structural Biology Afternoon Session: Structure-Based Drug Design Siegfried Reich (Agouron Pharmaceuticals): The Use of Protein Structural Information in Drug Design and Development: Some Examples Keith Wilson (Vertex Pharmaceuticals): Structure-Based Drug Design: Reality over Hype Poster Session I Saturday 11th: Morning Session: Structural Molecular Biology Stephen Cusack (EMBL Grenoble): tRNA and Amino Acid Recognition by Aminoacyl-tRNA Synthetases Robert Kaptein (Utrecht University): Allosteric Interactions in Protein-DNA Recognition Christoph Kratky (Graz University): Structure and Mechanism of Enzymes with a B12 Cofactor Paul Sigler (Yale University): Structure and Function in Chaperonin-Assisted Protein Folding Afternoon: Poster Session II (followed by the ´´Oldtimer-Tram´´ tour) Sunday 12th: Morning Session: Structural Genomics David Eisenberg (UCLA): Protein-Protein Interactions Terry Gaasterland (Rockefeller University): Using Patterns of Evolution Across Whole Genomes to Support Structural Genomics Target Selection John Moult (University of Maryland): The Past, Present, and Future of Protein Structure Prediction Wayne Hendrickson (Columbia University): Prospects of High-Throughput Crystallographic Structure Determination Approx. 1 p.m.: End of Conference with Farewell Drink and Snack ************************************************************************ Call for Posters Interested participants are invited to submit abstracts describing original work which has not been presented elsewhere. Abstracts should arrive no later than July 10th, 1999. Authors will be informed about the provisional acceptance of the abstract by the end of July. The presentation of the abstract as a poster will be confirmed, and included in the Book of Abstracts when one or more of the authors registers for the conference. Contributors of outstanding abstracts will be chosen by the Scientific Committee to give a 15 minute oral presentation. Poster Prize A prize of ATS 2,000 will be awarded for the best poster contribution i n terms of innovative results and presentation. ************************************************************************ Abstract Submission Camera-ready abstracts should be printed in good quality on a single (A4) sheet of paper, within the area 16 cm wide and 24 cm long. The title should be followed by the author(s) name(s), affiliation(s), and address(es). Text should be in a 12 point font with maximum 1.5 line spacing. Please send two unfolded copies of the abstract to the conference secretariat. ************************************************************************ Posters The poster boards will be 100 cm (width) x 200 cm (height). Materials for poster mounting will be provided. ************************************************************************ Conference Proceedings The lectures and poster presentations will be published by the Austrian Chemical Society in book form (with an ISBN number), and will be distributed to the participants upon arrival at the conference. Additional copies of the Conference Proceedings can be purchased for ATS 300,-. ************************************************************************ General Information Exhibition: An exhibition of instruments, accessories, software, literature, and other items is planned. Companies interested in displaying their products are kindly requested to contact the conference secretariat. Social Events: The conference will be opened by a welcome drink f ollowing the honorary lecture in the Federal Chancellery on the evening of Wednesday, September 8th. On Saturday afternoon, the conference participants will be taken on a tram tour of the city centre. Following this, all participants are encouraged to visit a typical Viennese Heurigen (wine cellar) to enjoy the local food and wine (not included in the registration fee). A further social event will be arranged for one other evening, leaving one evening free to explore Vienna. In addition, half day tours to some of Vienna´s best known sights will be organised for accompanying people (dependent on participant number). Registration (please contact the Conference Secretariat or our homepage): Registration Fee Before August 1 After August 1 Regular Participant 5.000 ATS 5.500