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From: "Gary" <gkrause@wayne.edu>
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Subject: Phosphatase reaction on nitrocellulose
Date: Mon, 3 Jan 2000 11:06:29 -0500
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Does anyone know of a protocol for performing a phosphatase (CIAP) reaction
on proteins bound to nitrocellulose?  Thanks.

Gary


--
Gary S. Krause
Wayne State University
Deprtment of Emergency Medicine
Detroit, MI 48201 USA




From owner-proteins@hgmp.mrc.ac.uk  Mon Jan  3 17:53:23 2000
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From owner-proteins@hgmp.mrc.ac.uk  Mon Jan  3 21:43:28 2000
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From: Amplicom <mshihNOmsSPAM@amplicom.com.invalid>
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From owner-proteins@hgmp.mrc.ac.uk  Tue Jan  4 04:09:22 2000
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From: jenny <jennyctpNOjeSPAM@yahoo.com.invalid>
Subject: protein determination- Lowry's method
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dear all,

i'm  jenny from malaysia and currently doing research on
effects of heavy metals on water lily. one of my studies is
to determine the growth effect and my parameter is to
estimate the protein content of the plant before and after
exposure to the heavy metals.

i read about Lowry's method which is said to be sensitive to
low concentrations of protein. i would like to know more
about the accuracy of this method as it said to be acurrate
at a certain pH range.

the other question is, can i determine the protein content
directly using dry samples (grinded leaves / roots) without
doing the protein extraction as i only want an approximate
result?

your comments and ideas are much appreciated.

thank you

jenny






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From owner-proteins@hgmp.mrc.ac.uk  Tue Jan  4 16:40:10 2000
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From: Daniel M Brown <daniel.m.brown@bms.com>
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Subject: Re: pH question!
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God, this takes me all the way back to Chemistry 101 a million
years ago.  pH is shorthand for hydrogen potential, although I
believe the original terminology was German, and it's been so
long ago that I don't recall the German.

Vernon Drake wrote:

> Could someone tell me what the letters pH stand for?  Not the
> definetion or what pH is. I think it means the POWER of
> HYDROGEN but I am not quite sure.
> vdrake@wgn.net

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From owner-proteins@hgmp.mrc.ac.uk  Tue Jan  4 17:29:24 2000
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From: Frank Fuerst <ffrank@rz.uni-potsdam.de>
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Subject: Re: pH question!
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Daniel M Brown wrote:
> 
> Vernon Drake wrote:
> 
> > Could someone tell me what the letters pH stand for?
> 
> God, this takes me all the way back to Chemistry 101 a million
> years ago.  pH is shorthand for hydrogen potential, although I
> believe the original terminology was German, and it's been so
> long ago that I don't recall the German.
 
Well, I'm german, and there is no word for hydrogen in german that
begins with an h, it's called "Wasserstoff". Also Potential is now a
german word, but is still recognized as a foreign word. And if "101 a
million years ago" is well before 1900, then latin was the language of
science in Germany (and elsewhere), so pH should be latin, too (even
if first used by german scientists) and thus be "pons" or "potentialis
hydrogenii"

Frank
-- 
Flhacs wird im Usenet grundsätzlich alsfhc geschrieben. Schreibt man
lafhsc nicht slfach, so ist das schlichtweg hclafs. Hingegen darf man
rihctig ruhig rhitcgi schreiben, weil eine shcalfe Schreibweise bei
irhictg nicht als shflac angesehen wird. [Hajo Pflüger in dnq]


From owner-proteins@hgmp.mrc.ac.uk  Tue Jan  4 17:38:56 2000
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From: Ulf Neumann <neumann@poboxes.com>
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In <028e001a.5e66e078@usw-ex0108-062.remarq.com> jenny wrote:

>i read about Lowry's method which is said to be sensitive to
>low concentrations of protein. i would like to know more
>about the accuracy of this method as it said to be acurrate
>at a certain pH range.

Have a look at

Peterson, G.L. (1979) Analytical Biochemistry _100_, 201-220

Regards,

Ulf Neumann


From owner-proteins@hgmp.mrc.ac.uk  Wed Jan  5 14:06:44 2000
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From: pathos@freerealtime.com (pathos)
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In article <84tal2$11r9u$1@fu-berlin.de>, Frank Fuerst
<ffrank@rz.uni-potsdam.de> wrote:

> Well, I'm german, and there is no word for hydrogen in german that
> begins with an h, it's called "Wasserstoff". Also Potential is now a
> german word, but is still recognized as a foreign word. And if "101 a
> million years ago" is well before 1900, then latin was the language of
> science in Germany (and elsewhere), so pH should be latin, too (even
> if first used by german scientists) and thus be "pons" or "potentialis
> hydrogenii"

I said this earlier in the thread. To be honest, I had no idea what it
meant because I am greek and know no latin. Now I know what the  word
"pons" means.  I believe it is time for me to get a latin dictionary.

Peter Pediaditakis


From owner-proteins@hgmp.mrc.ac.uk  Wed Jan  5 16:49:30 2000
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pathos schrieb:
> 
> > pH should be latin, too (even
> > if first used by german scientists) and thus be "pons" or "potentialis
> > hydrogenii"
> 
> I said this earlier in the thread. To be honest, I had no idea what it
> meant because I am greek and know no latin. Now I know what the  word
> "pons" means.  I believe it is time for me to get a latin dictionary.

Oh, I'm sorry, it should be pondus, weight.

Frank
-- 
Flhacs wird im Usenet grundsätzlich alsfhc geschrieben. Schreibt man
lafhsc nicht slfach, so ist das schlichtweg hclafs. Hingegen darf man
rihctig ruhig rhitcgi schreiben, weil eine shcalfe Schreibweise bei
irhictg nicht als shflac angesehen wird. [Hajo Pflüger in dnq]


From owner-proteins@hgmp.mrc.ac.uk  Wed Jan  5 18:10:08 2000
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In article <3861193C.C13E6AD4@wgn.net> vdrake@wgn.net "Vernon Drake" writes:
> 
> Could someone tell me what the letters pH stand for?  Not the definetion
> or what pH is. I think it means the POWER of HYDROGEN but I am not quite
> sure.
> vdrake@wgn.net
> 

I'm told this very question is answered in Trends in Biochemical
Sciences, January 2000 issue.  Still waiting for it to appear on
the web.

-Paul
-- 

--------------------------------------------------------------------
Paul Davis                                   paul@pdchem.demon.co.uk



From owner-proteins@hgmp.mrc.ac.uk  Wed Jan  5 20:17:39 2000
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From: yeyan@hotmail.com ("Yeyan Zhang")
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Hi, Everyone:

I am looking for a protocol that can purify his-tag proteins under denatured 
conditions, preferably with his-tag columns. Any info will be highly 
appreciated.

-Yeyan
______________________________________________________
Get Your Private, Free Email at http://www.hotmail.com

---


From owner-proteins@hgmp.mrc.ac.uk  Wed Jan  5 21:33:09 2000
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From: "Arnoud van Vliet" <avvliet@knoware.nl>
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Subject: Re: His-tag protein purification
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Look at www.qiagen.com for their brochure "Qiaexpressionist", or go to
www.invitrogen.com and look for the pTrcHis vectors an the tech info

no affiliations
hope this helps
Arnoud







From owner-proteins@hgmp.mrc.ac.uk  Wed Jan  5 22:03:11 2000
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Hi,

does anybody know the concentration of nickel in Brucella broth or other
(comparable) microbiological media? Or a protocol how to measure it, without
specific equipment; a colorimetric assay, preferably. My searches in the
Medline only resulted in a xylenol orange assay, but that's not specific for
nickel.

While I am at it, does anybody know a nickel specific chelator, so I can
measure the effect of varying the nickel conc on my pet microbe?

Thanks in advance,
Arnoud

--
Dr. A.H.M. van Vliet
Vrije Universiteit
Amsterdam
The Netherlands

email: avvliet@knoware.nl







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From: klenchin@REMOVE_TO_REPLY.facstaff.wisc.edu (Dima Klenchin)
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In article <947095658snz@pdchem.demon.co.uk>, Paul@pdchem.demon.co.uk wrote:
>In article <3861193C.C13E6AD4@wgn.net> vdrake@wgn.net "Vernon Drake" writes:
>> 
>> Could someone tell me what the letters pH stand for?  Not the definetion
>> or what pH is. I think it means the POWER of HYDROGEN but I am not quite
>> sure.
>> vdrake@wgn.net
>> 
>
>I'm told this very question is answered in Trends in Biochemical
>Sciences, January 2000 issue.  Still waiting for it to appear on
>the web.
>
>-Paul

I can't wait for the journal so I took a quick trip to a dictionary
that has some limited etimology (http://www.m-w.com/cgi-bin/):

        Main Entry: pH
        Pronunciation: 'pE-'Ach
        Function: noun
        Etymology: German, from _Potenz_ power + H 
        Date: 1909

"Power" actually does make sense (as opposed to pons). 

        - Dima


From owner-proteins@hgmp.mrc.ac.uk  Thu Jan  6 00:36:48 2000
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'pH' stands for the Latin 'pondus hydrogenium', i.e., the weight/power of
hydrogen.

The term was coined and defined at the end of the 19th century by the Danish
chemist Sorensen (the 'o' in the name has a slash through it) and a
colleague, whose name I have forgotten.
They were  employed by the Carlsberg beer brewery in Copenhagen and  trying
to figure out the parameters causing bad brews. They discovered as one of
the most decisive parameters the hydrogen-ion concentration.

Microbiologists later discovered  that at high pH-values  yeasts do not grow
anaerobically and strains of the anaerobe bacterium Clostridium take over,
producing a rather foul smelling brew.

Cheers,

Achim
___________________________________________

"Dima Klenchin" <klenchin@REMOVE_TO_REPLY.facstaff.wisc.edu> wrote in
message news:850ids$i4m$1@news.doit.wisc.edu...
> In article <947095658snz@pdchem.demon.co.uk>, Paul@pdchem.demon.co.uk
wrote:
> >In article <3861193C.C13E6AD4@wgn.net> vdrake@wgn.net "Vernon Drake"
writes:
> >>
> >> Could someone tell me what the letters pH stand for?  Not the
definetion
> >> or what pH is. I think it means the POWER of HYDROGEN but I am not
quite
> >> sure.
> >> vdrake@wgn.net
> >>
> >
> >I'm told this very question is answered in Trends in Biochemical
> >Sciences, January 2000 issue.  Still waiting for it to appear on
> >the web.
> >
> >-Paul
>
> I can't wait for the journal so I took a quick trip to a dictionary
> that has some limited etimology (http://www.m-w.com/cgi-bin/):
>
>         Main Entry: pH
>         Pronunciation: 'pE-'Ach
>         Function: noun
>         Etymology: German, from _Potenz_ power + H
>         Date: 1909
>
> "Power" actually does make sense (as opposed to pons).
>
>         - Dima




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I should have known that a good Pilsner is also a good teacher.


In article <%rRc4.1266$Cj3.4839444@bunson.tor.sfl.net>, "Achim
Recktenwald, PhD" <ARecktenwald@StressGen.com> wrote:

> 'pH' stands for the Latin 'pondus hydrogenium', i.e., the weight/power of
> hydrogen.
> 
> The term was coined and defined at the end of the 19th century by the Danish
> chemist Sorensen (the 'o' in the name has a slash through it) and a
> colleague, whose name I have forgotten.
> They were  employed by the Carlsberg beer brewery in Copenhagen and  trying
> to figure out the parameters causing bad brews. They discovered as one of
> the most decisive parameters the hydrogen-ion concentration.
> 
> Microbiologists later discovered  that at high pH-values  yeasts do not grow
> anaerobically and strains of the anaerobe bacterium Clostridium take over,
> producing a rather foul smelling brew.

Peter Pediaditakis


From owner-proteins@hgmp.mrc.ac.uk  Thu Jan  6 15:21:34 2000
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From: Neil McKenna <nmckenna@ottawa.com>
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Subject: Re: His-tag protein purification
Date: Thu, 06 Jan 2000 10:13:33 -0500
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Try http://www.qiagen.com/literature/handbooks/qxp/qxp/qxp.pdf

It is their "qiaexpressionist" handbook for use with their Ni-NTA
column. I have no affiliation with them, but this handbook is excellent
for protocols and troubleshooting. 

Yeyan Zhang wrote:
> 
> Hi, Everyone:
> 
> I am looking for a protocol that can purify his-tag proteins under denatured
> conditions, preferably with his-tag columns. Any info will be highly
> appreciated.
> 
> -Yeyan
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
> 
> ---


From owner-proteins@hgmp.mrc.ac.uk  Thu Jan  6 19:40:08 2000
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Grow up lots of tropical beetles.

Otherwise use other insect cell expression systems.


"aukbell" <aukbellNOauSPAM@hotmail.com.invalid> wrote in message
news:2180e014.0b9e5e73@usw-ex0108-061.remarq.com...
> X is a gene found in a tropical beetle.
>
> X has theraputic values.
>
> X is a 70KDa glycoprotein whose active form is a trimer.
> Glycosylation is essential for its activities.
>
> Which methods could be used to produce large amounts of X.
>
>
> * Sent from AltaVista http://www.altavista.com Where you can also find
related Web Pages, Images, Audios, Videos, News, and Shopping.  Smart is
Beautiful




From owner-proteins@hgmp.mrc.ac.uk  Fri Jan  7 16:50:30 2000
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From: Sparky <sparkster@writeme.com>
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Subject: Re: protein determination- Lowry's method
Date: Fri, 07 Jan 2000 16:38:11 GMT
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Hi Jenny,

> the other question is, can i determine the protein content
> directly using dry samples (grinded leaves / roots) without
> doing the protein extraction as i only want an approximate
> result?

As far as I know, most protein determinations rely on absorbance in
solution. As a result, you're going to have to extract the protein,
because otherwise insoluble portions of the plant will float in solution
and interfere with the assay.  Unless you have perfectly even
pulverization of the source material, the effect on your sample will be
unpredictable, and the results obtained will not even be approximate.
You could theoretically wait for that to all settle out, but since most
protein assays are also on a set time course there's no guarantee that
all the floaters will fall before you have to check the absorbance.

Just as a matter of curiosity, are you planning to look at the general
amount of protein in a given weight, or are you planning to look at the
amount of a specific but common protein?

Sparky
--
"Violence is the last refuge of the incompetent."--Isaac Asimov


Sent via Deja.com http://www.deja.com/
Before you buy.


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From: Shawn Strande <strande@sdsc.edu>
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Subject: ISMB 2000: Second Call for Papers
Date: Fri, 07 Jan 2000 09:43:00 -0800
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                          SECOND CALL FOR PAPERS

                 The Eighth International Conference on
           Intelligent Systems for Molecular Biology (ISMB 2000)
                       August 19 - August 23, 2000
                        San Diego, California USA
                         http://ismb2000.sdsc.edu

Submission deadlines are rapidly approaching for paper, tutorial
and posters for the upcoming Eighth International Conference on
Intelligent Systems for Molecular Biology (ISMB 2000), August 19-23,
2000, UC San Diego, California USA.  Essential information is given
below, with additional details available at: http://ismb2000.sdsc.edu.

We look forward to your participation to help make this a successful
meeting.

Sincerely,

Phil Bourne
Program Chair, ISMB 2000

------------------------


Papers
------
Deadline for receipt: February 14, 2000
Papers should be a maximum of 12 pages, single-spaced, including title,
abstract, figures, tables, and bibliography. The first page should give
keywords, postal and electronic mailing addresses, telephone and fax
numbers. Submit papers electronically to ismb2000@sdsc.edu in either
postscript or pdf format. Papers will only be accepted electronically.
Complete formatting details are available at:
http://www.aaai.org/Publications/Author/authorinstructions.html
PAPERS NOT CONFORMING TO THESE GUIDELINES WILL BE RETURNED WITHOUT
REVIEW.

Tutorials
---------
Proposal deadlines: January 15 and February 15, 2000
ISMB 2000 welcomes proposals for half-day or full-day introductory or
advanced tutorials (to be given on Saturday, 19 August, prior to the
meeting). A one-paragraph proposal stating your qualifications, your
prospective audience, and the "content of the tutorial" is due on
January 15, 2000 (submit electronically to ismb2000@sdsc.edu). A full
3-page proposal is due on February 15, 2000. The program committee
will reply to tutorial proposers in April. Draft handouts for selected
tutorials must be submitted by May 1, 2000.

Posters and Software Demonstrations
-----------------------------------
Abstracts due: May 31, 2000
An abstract of your poster should be submitted by May 31, 2000,
including title, author(s), affiliation(s), e-mail address(es),
text, and references.  Poster space will be approximately 145 cm
(4 ft) high by 110 (3 ft) cm wide.  If you represent an academic
group and wish to demonstrate software, please notify the conference
organizers by sending e-mail to ismb2000@sdsc.edu as soon as possible.

Key Dates
---------
Meeting:
Tutorial presentations: Aug 19, 2000
Paper presentations: Aug 20-23, 2000

Paper Submissions:
Papers must be received by: Feb 14, 2000
Replies to authors by: Mar 20, 2000
Revised papers must be received by: Apr 10, 2000

Open Poster Submissions
Abstracts must be received by: May 31, 2000

Tutorial Proposals
Short (1-paragraph) proposals must be received by: Jan 15, 2000
Full (3 page) proposals must be received by: Feb 15, 2000
Replies to proposers by: Apr. 1, 2000
Draft handouts must be received by: May 1, 2000
Final handouts must be received by: Jul 01, 2000
Tutorials presented: Aug 19, 2000

Registration
Begins: Apr. 15, 2000 Ends: June 15, 2000




From owner-proteins@hgmp.mrc.ac.uk  Sat Jan  8 03:41:16 2000
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From: Liu Xiangyu <liuxiangyu@hotmail.com>
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Subject: pfu and taq PCR mutation percentage
Date: Fri, 07 Jan 2000 22:27:57 -0600
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Hi, All, I am constructing a expression vecter and having a question
about fidelity
                 comparison of Taq and pfu polymerase
for Taq: error rate is 8.0 X 10^-6   For pfu: ..... is 1.3 X 10^-6
                 According to stratagene pfu DNA polymerase manual, the
percentage of mutated PCR products after amplification of a 1KB target
seq for 20 cycles are 2.6% for pfu and 16.0% for Taq.
                 Does anybody knows a simple formula to caculate? for
example, what happens to 3 KB target after 10 cycles by pfu or taq?
                 If I use Taq for 10 cycles and pfu for 20 cycles( since
pfu always give much weak  bands), which one gives better result
considering percentage of mutation.
                Thank you for your help.
                 Liu



From owner-proteins@hgmp.mrc.ac.uk  Sat Jan  8 12:53:50 2000
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------=_NextPart_000_2FA3_00002F4A.00004CC3
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From owner-proteins@hgmp.mrc.ac.uk  Sat Jan  8 17:22:22 2000
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LEGAL C.A`B`L`E TV D`E-S`C`R`A`M`B`L`E`R

Want to watch Sporting Events?--Movies?--Pay-Per-View??

*This is the Famous R-O Shack TV Descrambler 
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2. E-Z To read Original Drawings. 
3. Total Parts List.

**** PLUS SOMETHING NEW YOU MUST HAVE! ****
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along with your $10.00 payment to:

Cabletron FREE-TV
12187 S. Orange Blossom Trail #116
Orlando Fl 32837
 
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(Florida residents include 7% Florida State Sales Tax)
(All orders outside the U.S.A. add $5.00)


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We are NOT ASSOCIATED in any way with RADIO SHACK. 
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Parts for this fine-tuning device are available 
at many electronics stores (including Radio Shack) 
This is not a Radio Shack product.
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From owner-proteins@hgmp.mrc.ac.uk  Sun Jan  9 14:17:03 2000
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LEGAL C`A`B`L`E TV D`E-S`C`R`A`M`B`L`E`R

Want to watch Sporting Events?--Movies?--Pay-Per-View??....FREE!!!!

*This is the Famous R-O Shack TV D-e-s-c-r-a-m-b-l-e-r 
You can assemble it from Radio Shack parts for about $12 to $15.

We Send You: 
1, E-Z To follow Assembly Instructions. 
2. E-Z To read Original Drawings. 
3. Total Parts List.

**** PLUS SOMETHING NEW YOU MUST HAVE! ****
Something you can't do without.

THE UP-TO-DATE 6 PAGE REPORT: 
USING A D-E-S-C-R-A-M-B-L-E-R LEGALLY

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without reading this report first.

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and instruction package including
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follow diagram, and most important 
of all the "Using a D`e`s`c`r`a`m`b`l`e`r
LEGALLY Report all for just--$10.00 

Fill out form below and send it,
along with your $10.00 payment to:

C.a.b.l.e.t.r.o.n FREE-TV
12187 S. Orange Blossom Trail #116
Orlando Fl 32837
 
(Cash, Check or Money Order.)
(Florida residents include 7% Florida State Sales Tax)
(All orders outside the U.S.A. add $5.00)


PRINT YOUR:

NAME______________________________________________________

ADDRESS___________________________________________________

CITY/STATE/ZIP____________________________________________

E-MAIl ADDRESS____________________________________________ 



rtg45t34t43t34t34t43t34t34t34t34t34t34t34
idfmgqxpgtpwooilxnkscigqggqdwoimwlkpwunuttzmozi



From owner-proteins@hgmp.mrc.ac.uk  Sun Jan  9 23:18:02 2000
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From: johns@ks.uiuc.edu (John Stone)
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Subject: Announce: VMD 1.4
Date: 9 Jan 2000 23:17:54 GMT
Organization: University of Illinois at Urbana-Champaign
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         VMD "Visual Molecular Dynamics" 1.4 Announcement
         ------------------------------------------------
The Theoretical Biophysics group at the Beckman Institute For Advanced  
Science and Technology, the University of Illinois (U-C), is proud to
announce the public release of VMD 1.4.  VMD is a package for the 
visualization and analysis of biomolecular systems.  This software is
distributed free of charge and includes source code, documentation,
and precompiled binaries for IBM, HP, Linux, Sun, and SGI Unix systems, 
as well as Microsoft Windows 95/98/NT.
The VMD documentation includes an installation guide, a users guide, and a 
programmers guide for interested researchers.  VMD also provides on-line 
help through the use of an external HTML viewer.  VMD development is 
supported by the NIH National Center for Research Resources.

A full description of VMD is available via the VMD WWW home page:
        http://www.ks.uiuc.edu/Research/vmd/

The authors request that any published work which utilizes VMD includes 
a reference to the VMD web page and/or the following reference:

  Humphrey, W., Dalke, A. and Schulten, K., "VMD - Visual Molecular
  Dynamics", J. Molec. Graphics, 1996, vol. 14, pp. 33-38.

The Theoretical Biophysics group encourages VMD users to be closely  
involved in the development process through reporting bugs, contributing  
fixes, periodical surveys and via other means.

We are eager to hear from you, and thank you for using our software!

                                                John Stone
                                                vmd@ks.uiuc.edu
                                                January 7, 2000


-- 

Theoretical Biophysics Group   Email: johns at ks.uiuc.edu
Beckman Institute              http://www.ks.uiuc.edu/~johns/
University of Illinois         Phone:  (217) 244-3349
405 N. Mathews  Ave              FAX:  (217) 244-6078 
Urbana, IL 61801, USA          Unix Is Good For You!!!



From owner-proteins@hgmp.mrc.ac.uk  Mon Jan 10 02:00:13 2000
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Subject: Re: pH question!
Date: Mon, 10 Jan 2000 01:46:19 GMT
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In article <947095658snz@pdchem.demon.co.uk>,
  Paul@pdchem.demon.co.uk wrote:
> In article <3861193C.C13E6AD4@wgn.net> vdrake@wgn.net "Vernon Drake" writes:
> >
> > Could someone tell me what the letters pH stand for?  Not the definetion
> > or what pH is. I think it means the POWER of HYDROGEN but I am not quite
> > sure.
> > vdrake@wgn.net
> >
>
> I'm told this very question is answered in Trends in Biochemical
> Sciences, January 2000 issue.  Still waiting for it to appear on
> the web.
>
> -Paul
> --
>
> --------------------------------------------------------------------
> Paul Davis                                   paul@pdchem.demon.co.uk
>
>

Paul, PH stans for the acid or Alchaline in the water it is ususally used in
the maintaining a certain PH Factor for raising Fish in a Aquarium. I know i
still have a 20 Gallon High, for Tropical Fish, My Favorites are certain
types of a hard to grow catfish that I raise and sell. imforce



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This is a multi-part message in MIME format.
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Proteome Inc. is a rapidly growing, dynamic company that is dedicated to

providing high-quality, comprehensively curated
databases to the biotechnology and pharmaceutical industries, as well as

to the academic research community.  We work at the
forefront of the bioinformation revolution, integrating genomic data
with data from the published literature.  See our Web site at
http://www.proteome.com

Proteome employees work in an energetic and intellectually stimulating
environment.  Our positions of Scientific Curator and
Database Editor are unique.  We offer competitive salaries and benefits,

and excellent opportunities for advancement in our
expanding company. In addition, Proteome's job options -- working full
or part time, either on site or remotely via the
internet -- offer maximum personal flexibility.  Proteome Inc.
encourages diversity in its workplace and is an equal
opportunity employer.

Job Openings

Scientific Curator:

Proteome, Inc. is seeking full-time and part-time Scientific Curators to

collect and integrate protein information for
multispecies databases. Successful candidates will have an opportunity
to work in a broad range of topics that is unparalleled in academia or
in industry.  Candidates must have a PhD and specific training or
laboratory experience in mammalian, yeast,
fungal or other model systems. Candidates must have excellent writing
skills and demonstrate knowledge of a wide spectrum
of topics in cell biology or related fields.  A strong interest in
organizing biological information is an asset. Opportunities
exist for advancement to the position of Scientific Editor as well as to

other positions of responsibility within Proteome, Inc.

Database Editor:

Proteome, Inc. is seeking full-time and part-time Database Editors to
develop and oversee the construction of protein
databases. Successful candidates will work with comprehensive data sets
that are derived from both genomic and classical
experiments and fully describe our current understanding of cell and
organismal biology.  Candidates must have a PhD and
specific training or laboratory experience in mammalian, yeast, fungal
or other model systems. Candidates must demonstrate
knowledge of a broad range of topics in cell biology or related fields.
Excellent writing skills, an eye for detail, and a strong
interest in organizing biological information are required.  Candidates
must have good problem-solving abilities and must be
capable of overseeing the work of several Scientific Curators. Computer
skills, including familiarity with the UNIX
environment and with SQL and Perl, are an asset but are not necessary.
Opportunities exist for advancement to other positions of responsibility

within Proteome, Inc.

Please send your CV to:
Human Resources
PROTEOME INC.
100 Cummings Center
Suite 435M
Beverly, MA 01915
TEL (978) 922-1643
FAX (978) 922-3971
email: rgh@proteome.com
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Message-ID: <385BFB74.193D4367@proteome.com>
Date: Sat, 18 Dec 1999 16:24:08 -0500
From: "Brian P. Davis" <bpd@proteome.com>
Reply-To: bpd@proteome.com
Organization: Proteome, Inc.
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Subject: Unique Scientific Positions at Proteome, Inc.
Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353"
Content-Transfer-Encoding: 7bit

Proteome Inc. is a rapidly growing, dynamic company that is dedicated to
providing high-quality, comprehensively curated
databases to the biotechnology and pharmaceutical industries, as well as
to the academic research community.  We work at the
forefront of the bioinformation revolution, integrating genomic data
with data from the published literature.  See our Web site at
http://www.proteome.com

Proteome employees work in an energetic and intellectually stimulating
environment.  Our positions of Scientific Curator and
Database Editor are unique.  We offer competitive salaries and benefits,
and excellent opportunities for advancement in our
expanding company. In addition, Proteome's job options -- working full
or part time, either on site or remotely via the
internet -- offer maximum personal flexibility.  Proteome Inc.
encourages diversity in its workplace and is an equal
opportunity employer.

Job Openings

Scientific Curator:

Proteome, Inc. is seeking full-time and part-time Scientific Curators to
collect and integrate protein information for
multispecies databases. Successful candidates will have an opportunity
to work in a broad range of topics that is unparalleled in academia or
in industry.  Candidates must have a PhD and specific training or
laboratory experience in mammalian, yeast,
fungal or other model systems. Candidates must have excellent writing
skills and demonstrate knowledge of a wide spectrum
of topics in cell biology or related fields.  A strong interest in
organizing biological information is an asset. Opportunities
exist for advancement to the position of Scientific Editor as well as to
other positions of responsibility within Proteome, Inc.

Database Editor:

Proteome, Inc. is seeking full-time and part-time Database Editors to
develop and oversee the construction of protein
databases. Successful candidates will work with comprehensive data sets
that are derived from both genomic and classical
experiments and fully describe our current understanding of cell and
organismal biology.  Candidates must have a PhD and
specific training or laboratory experience in mammalian, yeast, fungal
or other model systems. Candidates must demonstrate
knowledge of a broad range of topics in cell biology or related fields.
Excellent writing skills, an eye for detail, and a strong
interest in organizing biological information are required.  Candidates
must have good problem-solving abilities and must be
capable of overseeing the work of several Scientific Curators. Computer
skills, including familiarity with the UNIX
environment and with SQL and Perl, are an asset but are not necessary.
Opportunities exist for advancement to other positions of responsibility
within Proteome, Inc.

Please send your CV to:
Rebecca Holbrook
PROTEOME INC.
100 Cummings Center
Suite 435M
Beverly, MA 01915
TEL (978) 922-1643
FAX (978) 922-3971
email: rgh@proteome.com
--
Regards,

((((((((((((((((((((((((((((((((((((((((((((()))))))))))))))))))))))))))))))))))))))))))))

Brian P. Davis, Ph.D.                            Director of Database
Development
PROTEOME INC.                                         bpd@proteome.com
((((((((((()))))))))))(((((((((()))))))))))(((((((((((()))))))))))(((((((((()))))))))))))





--------------D8A325E9291023FAB18CB48E--

---


From owner-proteins@hgmp.mrc.ac.uk  Mon Jan 10 12:04:40 2000
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From: David Scott <djs17@york.ac.uk>
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Subject: Hemin Absorption coefficient
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Hia,
Does anyone know where I can find the absorption coefficient for Hemin:
oxidised/reduced, etc.

Thanks in advance

d.

--
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* YORK   *
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* Y01 5DD   *
* Phone: +44 1904 432868 *
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From owner-proteins@hgmp.mrc.ac.uk  Mon Jan 10 17:28:44 2000
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Dear Netters:
I tried to purify GST-fusion protein using Glutathione Sepharose 4B
(Pharmacia). The protein is 30kDa DNA-glycosylase With high affinity to
DNA. Finally, I've got a good band. However, about 80% of activity
remains in the crude extract. Re-use of the flow-through with new batch
of resin resulted in nothing-most of the activity goes through the
resin.How can I improve the binding of my protein? High salt? More
detergent? 
Any ideas will be highly appreciated.
Sincerely,
Cyril VP

-- 
Dr. Cyril V. Privezentzev
Group "Reparation de l'ADN
CNRS UMR 8532  
Institut Gustave-Roussy
Pavillon de Recherche II
39 rue Camille Desmoulins
94805 Villejuif Cedex
FRANCE
         
Tel. +33 (0)1 42 11 54 04
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From owner-proteins@hgmp.mrc.ac.uk  Tue Jan 11 09:05:02 2000
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From: gutberlet@hmi.de ("Thomas Gutberlet")
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Dear colleagues,

I like to introduce you to the following workshop, organized by the 
HMI Berlin, BENSC and MDC, on "Protein Crystallography with 
Neutrons" on Feb. 25.-26. 2000 in Berlin at the Institute of Medical
Physics and Biophysics. The aim of the workshop is to discuss 
current use of protein crystallography with neutrons, advantages 
and disadvantages of neutrons in this field and future prospects with 
respect to new opportunities and facilities as ESS. A current 
program is available at 
http://www.hmi.de/bensc/workshop/proteins.html. There is no 
conference fee. For additional information you may send me an e- 
mail gutberlet@hmi.de.

Kind regards
Thomas Gutberlet

Dr. Thomas Gutberlet
Project Assistant ESS Instrumentation
Hahn-Meitner-Institut Berlin GmbH
Abt. NE
Glienicker Str. 100
14109 Berlin, Germany

Tel.: **49/30/8062-3159
Fax.: **49/30/8062-2999
e-mail gutberlet@hmi.de
http://www.hmi.de/people/gutberlet/
http://www.kfa-juelich.de/ess/ess.html
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From owner-proteins@hgmp.mrc.ac.uk  Tue Jan 11 15:16:05 2000
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Subject: Re: pH question!
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pH stands for pondus Hydrogenii. pondus is the latin word
for amount, load or mass. Hydrogenii comes from the latin
word for hydrogen. So, translated word by word pH is the
abbreviation for "amount of hydrogen".


* Sent from AltaVista http://www.altavista.com Where you can also find related Web Pages, Images, Audios, Videos, News, and Shopping.  Smart is Beautiful


From owner-proteins@hgmp.mrc.ac.uk  Wed Jan 12 10:20:15 2000
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From: vishwas101@yahoo.com
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Subject: Glucose Isomerase needs your help to come out
Date: Wed, 12 Jan 2000 10:13:23 GMT
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Dear Netters,

I am trying to study Glucose isomerase from fruits.

If anyone ever tried the same please help me.

I need extraction protocols as well as some suggestions for some more
good sources.

Thanks in advance






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Subject: trypan-blue-labelled albumin?
Date: Wed, 12 Jan 2000 10:48:03 -0000
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Anyone know a UK supplier for trypan-blue labelled albumin? Or a protocol
for labelling albumin with trypan blue ourselves?

Thanks for any info.

Samuel Gray
Division of Vascular Medicine,
Derbyshire Royal Infirmary,
Derby,
UK.





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From: leemor@cshl.org (Leemor Joshua-Tor)
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Subject: Postdoctoral position
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--============_-1264401902==_ma============
Content-Type: text/plain; charset="us-ascii"

POSTDOCTORAL POSITION in STRUCTURAL BIOLOGY
at COLD SPRING HARBOR


Applications are invited for a postdoctoral position in macromolecular
crystallography at Cold Spring Harbor Laboratory to work on complexes
involved in gene expression. The positions are available for highly
motivated applicants with a recent Ph. D. and experience in either protein
crystallography or a related field or those with experience in biochemistry
or molecular biology and a keen interest in protein crystallography and
structural biology. Experience in protein purification is an advantage.

Our X-ray crystallography facilities include a Rigaku generator, mirrors,
an R-axis detector, an Oxford cryosystem and several graphics workstations.
We share a beamline at the nearby National Synchrotron Light Source (NSLS)
at Brookhaven. We also have state-of-the-art facilities for carrying out
biochemical and molecular biology studies. Cold Spring Harbor provides a
unique stimulating and very collaborative environment for rich scientific
interactions. For details about CSHL please see our web site at:
http://www.cshl.org/

Please send CV, list of publications, a summary of research experience and
interests, and names and numbers of three references to Dr. Leemor
Joshua-Tor, Cold Spring Harbor Laboratory, P. O. Box 100, Cold Spring
Harbor, NY 11724. Tel. (516) 367 8821. For additional information or
informal inquiries, please call or send e-mail to leemor@cshl.org

*******************************************************************
  Leemor Joshua-Tor, Ph.D.
  Associate Professor
  Keck Structural Biology
  Cold Spring Harbor Laboratory           Tel. (516) 367 8821
  1 Bungtown Road                         Fax  (516) 367 8873
  Cold Spring Harbor, NY 11724            e-mail: leemor@cshl.org
*******************************************************************
--============_-1264401902==_ma============
Content-Type: text/enriched; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable

<bold><italic><fontfamily><param>Helvetica</param><bigger><bigger>POSTDOCTOR=
AL
POSITION in STRUCTURAL BIOLOGY

at COLD SPRING HARBOR



</bigger></bigger></fontfamily></italic></bold><fontfamily><param>Helvetica<=
/param><bigger>Applications
are invited for a postdoctoral position in macromolecular
crystallography at Cold Spring Harbor Laboratory to work on complexes
involved in gene expression. The positions are available for highly
motivated applicants with a recent Ph. D. and experience in either
protein crystallography or a related field or those with experience in
biochemistry or molecular biology and a keen interest in protein
crystallography and structural biology. Experience in protein
purification is an advantage.=20


Our X-ray crystallography facilities include a Rigaku generator,
mirrors, an R-axis detector, an Oxford cryosystem and several graphics
workstations. We share a beamline at the nearby National Synchrotron
Light Source (NSLS) at Brookhaven. We also have state-of-the-art
facilities for carrying out biochemical and molecular biology studies.
Cold Spring Harbor provides a unique stimulating and very collaborative
environment for rich scientific interactions. For details about CSHL
please see our web site at: http://www.cshl.org/


Please send CV, list of publications, a summary of research experience
and interests, and names and numbers of three references to <bold>Dr.
Leemor Joshua-Tor, Cold Spring Harbor Laboratory, P. O. Box 100, Cold
Spring Harbor, NY 11724. Tel. (516) 367 8821</bold>. For additional
information or informal inquiries, please call or send e-mail to
leemor@cshl.org </bigger></fontfamily>



*******************************************************************

  Leemor Joshua-Tor, Ph.D.

  Associate Professor

  Keck Structural Biology                      =20

  Cold Spring Harbor Laboratory           Tel. (516) 367 8821

  1 Bungtown Road                         Fax  (516) 367 8873

  Cold Spring Harbor, NY 11724            e-mail: leemor@cshl.org

*******************************************************************

--============_-1264401902==_ma============--
---


From owner-proteins@hgmp.mrc.ac.uk  Thu Jan 13 13:39:07 2000
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From: athel@ir2cbm.cnrs-mrs.fr (Athel Cornish-Bowden)
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Subject: Re: pH question!
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Achim wrote:

>'pH' stands for the Latin 'pondus hydrogenium', i.e., the weight/power of
>hydrogen.
>
>The term was coined and defined at the end of the 19th century by the Danish
>chemist Sorensen (the 'o' in the name has a slash through it) and a
>colleague, whose name I have forgotten.
>
>
The paper only has one name on it (Sorensen's), so I don't think there is
someone you've forgotten. It appeared simultaneously in German (Biochem. Z.
21, 131-304 (1909)), French (Compt. rend. Laboratory. Carlsberg 8, 1-174
(1909)) and Danish (Meddelser fra Carlsberg Laboratoriet 8, 153
(1909-1910)), so you can take your pick as to which you regard as the
primary source. According to Tom Boyde, who translated the French version
into English for "Foundation Stones of Biochemistry" (Voile et Aviron, Hong
Kong, 1980), pp. 156-262, the three versions are not the same. (I don't
suppose he read the Danish, but the French and German versions are
different).

The person you might (conceivably) have forgotten is Michaelis, who was not
a coauthor and was not involved in introducing the symbol pH, but who had
been working on very similar lines and built on Sorensen's work extremely
soon after it was published. In 1914 he published what for a while was the
standard book, Die Wasserstoffionenkonzentration.

Athel Cornish-Bowden



================================================================

Athel Cornish-Bowden

Bioenergetique et Ingenierie des Proteines,
Centre National de la Recherche Scientifique,
31 chemin Joseph-Aiguier, B.P. 71,
13402 Marseille Cedex 20, France (CHANGED 1.1.2000)

athel@ibsm.cnrs-mrs.fr
Phone: + 33 491 16 41 38; fax: + 33 491 16 45 78 (CHANGED)

http://ir2lcb.cnrs-mrs.fr/~athel/homepage.htm

Now available: Basic Mathematics for Biochemists (2nd edn.)
http://ir2lcb.cnrs-mrs.fr/~athel/basmaths.htm


---


From owner-proteins@hgmp.mrc.ac.uk  Thu Jan 13 17:16:10 2000
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From: Nick Theodorakis <nicholas_theodorakis@urmc.rochester.edu>
Subject: Re: pH question!
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In article <v03007807b4a37fca799b@[193.50.234.80]>,
athel@ir2cbm.cnrs-mrs.fr (Athel Cornish-Bowden) wrote:

[...]

> The paper only has one name on it (Sorensen's), so I don't think
> there is
> someone you've forgotten. It appeared simultaneously in German
> (Biochem. Z.
> 21, 131-304 (1909)), French (Compt. rend. Laboratory. Carlsberg 8,
> 1-174
> (1909)) and Danish (Meddelser fra Carlsberg Laboratoriet 8, 153
> (1909-1910)), so you can take your pick as to which you regard as
> the
> primary source.

[...]

Now _that_ is one way to increase the length of one's cv! ;-)

Nick



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From owner-proteins@hgmp.mrc.ac.uk  Fri Jan 14 01:00:53 2000
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a method that takes less setting up than a lowry is one
using a bradford reagent, it still relys on absorbance but
you only have one solution (bradford soln) to add to your
protein sample, that i reckon would have to be free of
particulate matter.


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From owner-proteins@hgmp.mrc.ac.uk  Fri Jan 14 17:15:10 2000
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From: "Laurent Terradot" <terradot@rennes.inra.fr>
X-Newsgroups: bionet.molbio.proteins
Subject: peritrophins
Date: Fri, 14 Jan 2000 17:09:08 +0100
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Dear Netter,

I would like to have information about peritrophin. Does anyone know if some
of these proteins have been described in homopterae insects ?
Is there some antibodies available ?

thank you for your help
best regards,
Laurent

Laurent Terradot
INRA, Station de pathologie végétale
BP29, 35653 Le Rheu cedex
terradot@rennes.inra.fr





From owner-proteins@hgmp.mrc.ac.uk  Sat Jan 15 17:22:40 2000
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From: "Sylvain Kuony" <skuony@club-internet.fr>
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Subject: Ethanol-chloroform, need help
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I have performed an EtOH-Chloroform precipitation to get a protein, but most
of my protein was lost. Why? Is it important to perform the precipitation
with ethanol-chloroform at 0 C degree?
Thanx




From owner-proteins@hgmp.mrc.ac.uk  Sun Jan 16 16:50:48 2000
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Subject: Re: Ethanol-chloroform, need help
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What is your ratio of EtOH:chloroform?

I use a 2.5 vol excess of EtOH when precipitating protein
from a phenol phase. All at room temperature.

RWN


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From owner-proteins@hgmp.mrc.ac.uk  Sun Jan 16 20:59:33 2000
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I use a 1:1 ratio of EtOH-chloroform
. Do you know what could explain the loss of my protein?
S. Kuony
rwn <rwnNOrwSPAM@mbio.aau.dk.invalid> a écrit dans le message :
13001ab4.2578afb6@usw-ex0108-062.remarq.com...
> What is your ratio of EtOH:chloroform?
>
> I use a 2.5 vol excess of EtOH when precipitating protein
> from a phenol phase. All at room temperature.
>
> RWN
>
>
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From owner-proteins@hgmp.mrc.ac.uk  Sun Jan 16 23:20:55 2000
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Well, this is not my strongest side, but I believe that your
protein could simply be soluble in the chloroform phase
(like I use a phenol phase to extract protein).

Unless you get better suggestions I would start by adding
more EtOH. If you have enough protein, you can actually see
it precipitate out at the critical EtOH vol.

good luck, rwn



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From owner-proteins@hgmp.mrc.ac.uk  Mon Jan 17 19:49:49 2000
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Just go to the Qiagen web site and download the qiaexpressionist hand book.
You can download all you need from their web pages on his-tagged proteins.
Their reagents worked great for producing a fusion for antibody production for
me.
PCPhD

Yeyan Zhang wrote:

> Hi, Everyone:
>
> I am looking for a protocol that can purify his-tag proteins under denatured
> conditions, preferably with his-tag columns. Any info will be highly
> appreciated.
>
> -Yeyan
> ______________________________________________________
> Get Your Private, Free Email at http://www.hotmail.com
>
> ---



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Laurent Terradot wrote:

> Dear Netter,
>
> I would like to have information about peritrophin. Does anyone know if some
> of these proteins have been described in homopterae insects ?
> Is there some antibodies available ?
>
> thank you for your help
> best regards,
> Laurent
>
> Laurent Terradot
> INRA, Station de pathologie végétale
> BP29, 35653 Le Rheu cedex
> terradot@rennes.inra.fr

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[Image]                                [Image]

   PubMed     Nucleotide    Protein      Genome     Structure      PopSet
 Search  for
[Image] Limits     Index     History   Clipboard
About           [Image]
Entrez                                    Show:                    One page.
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         [Image]                     1-5 items of 5

Entrez PubMed          1 : Insect Biochem Mol Biol 1999    Related Articles,
Search                 Apr;29(4):319-27                      Books, LinkOut
Overview
Help | FAQ                  A peritrophin-like protein expressed in the
                            embryonic tracheae of Drosophila melanogaster.
PubMed Services
Journal Browser             Barry MK, Triplett AA, Christensen AC
MeSH Browser
Single Citation             School of Biological Sciences, University of
Matcher                     Nebraska, Lincoln 68588-0118, USA.
Batch Citation
Matcher                     We have cloned and sequenced a cDNA from
Clinical                    Drosophila melanogaster that encodes a protein
Queries                     homologous to the peritrophins, a family of
                            chitin-binding proteins from the peritrophic
Related                     matrix of insects. Unexpectedly, the gene, Gasp,
Resources                   is expressed in the embryonic tracheae. We
Order Documents             suggest that this family of proteins may be
Grateful Med                present in other tissues than the peritrophic
Consumer Health             matrix, particularly where nutrient or gas
                            exchange are important, and/or where invasion by
                            parasites or viruses is possible. We have also
                            mapped two similar genes that had been sequenced
                            by the Berkeley Drosophila Genome Project, and
                            find that these three very similar genes are not
                            clustered, but are located on three different
                            chromosomes.

                            PMID: 10333571, UI: 99266113

                       ------------------------------------------------------

                       2 : J Biol Chem 1998 Jul            Related Articles,
                       10;273(28):17665-70                   Books, Protein,
                                                        Nucleotide, LinkOut
                            [Image]
                            A type I peritrophic matrix protein from the
                            malaria vector Anopheles gambiae binds to
                            chitin. Cloning, expression, and
                            characterization.

                            Shen Z, Jacobs-Lorena M

                            Case Western Reserve University, School of
                            Medicine, Department of Genetics, Cleveland,
                            Ohio 44106-4955, USA.

                            Upon feeding, mosquito midguts secrete the
                            peritrophic matrix (PM), an extracellular
                            chitin-containing envelope that completely
                            surrounds the blood meal. Because the malaria
                            parasite must cross the PM to complete its life
                            cycle in the mosquito, the PM is a potential
                            barrier for malaria transmission. By antibody
                            screening of an expression library we have
                            identified and partially characterized a cDNA
                            encoding a putative PM protein, termed Anopheles
                            gambiae adult peritrophin 1 (Ag-Aper1). Ag-Aper1
                            is the first cloned PM gene from a disease
                            vector. Northern analysis detected an abundant
                            Ag-Aper1 transcript only in the adult gut, and
                            not in any other tissues or at any other stages
                            of development. The predicted amino acid
                            sequence indicates that it has two tandem
                            chitin-binding domains that share high sequence
                            similarity with each other and also with the
                            chitin-binding domain of an adult gut-specific
                            chitinase from the same organism. The presumed
                            ability of Ag-Aper1 to bind chitin was verified
                            by a functional assay with the
                            baculovirus-expressed recombinant protein.
                            Ag-Aper1 did bind to chitin but not to
                            cellulose, indicating that Ag-Aper1 binds chitin
                            specifically. The double chitin-binding domain
                            organization of Ag-Aper1 suggests that each
                            protein molecule is able to link two chitin
                            polymer chains. Hence, this protein is likely to
                            act as a molecular linker that connects PM
                            chitin fibrils into a three-dimensional network.

                            PMID: 9651363, UI: 98316335

                       ------------------------------------------------------

                       3 : Insect Biochem Mol Biol 1998    Related Articles,
                       Feb;28(2):99-111                               Books

                            cDNA and deduced amino acid sequences of a
                            peritrophic membrane glycoprotein,
                            'peritrophin-48', from the larvae of Lucilia
                            cuprina.

                            Schorderet S, Pearson RD, Vuocolo T, Eisemann C,
                            Riding GA, Tellam RL

                            CSIRO Tropical Agriculture, Queensland,
                            Australia.

                            The gut of most insects is lined with a
                            semi-permeable peritrophic membrane (or
                            peritrophic matrix) composed of chitin,
                            proteoglycans and proteins. Despite the probable
                            importance of the peritrophic membrane in
                            facilitating the digestive process and
                            protecting insects from invasion by
                            micro-organisms and parasites, there has been
                            little characterization of the specific
                            components and their interactions within this
                            acellular structure. Here we report the
                            characterization of an integral peritrophic
                            membrane glycoprotein, peritrophin-48, from the
                            larvae of the fly Lucilia cuprina, a primary
                            agent of cutaneous myiasis in sheep.
                            Peritrophin-48 was purified from peritrophic
                            membrane obtained by larval culture and its
                            location within the peritrophic membrane
                            determined by immuno-fluorescence and
                            immuno-gold localizations. The cDNA coding for
                            peritrophin-48 was cloned and sequenced. The
                            deduced amino acid sequence codes for a protein
                            of 375 amino acids containing an amino-terminal
                            signal sequence followed by five similar, but
                            non-identical domains, each approximately 65-70
                            amino acids in length and characterised by a
                            specific register of six cysteines. The deduced
                            amino acid sequence shows significant similarity
                            to two other peritrophic membrane proteins,
                            peritrophin-95 and peritrophin-44, from the same
                            species. A reverse transcriptase-PCR approach
                            indicated that there are several highly related
                            peritrophin-48 genes expressed in each
                            individual. Reverse transcriptase-PCR also
                            demonstrated the expression of peritrophin-48 in
                            all three larval instars and adults but not
                            pupae or eggs. Peritrophin-48 was expressed only
                            by the cardia and by the larval midgut. A simple
                            structural model of a basic unit of a type 2
                            peritrophic membrane is presented.

                            PMID: 9639876, UI: 98304029

                       ------------------------------------------------------

                       4 : Proc Natl Acad Sci U S A Related Articles, Books,
                       1997 Aug 19;94(17):8939-44       Protein, Nucleotide,
                                                                    LinkOut
                            [Image]
                            Antibody-mediated inhibition of the growth of
                            larvae from an insect causing cutaneous myiasis
                            in a mammalian host.

                            Casu R, Eisemann C, Pearson R, Riding G, East I,
                            Donaldson A, Cadogan L, Tellam R

                            Commonwealth Scientific and Industrial Research
                            Organization (CSIRO) Tropical Agriculture, CSIRO
                            P.M.B. 3, Indooroopilly, 4068 Queensland,
                            Australia.

                            Many insects feed on blood or tissue from
                            mammalian hosts. One potential strategy for the
                            control of these insects is to vaccinate the
                            host with antigens derived from the insect. The
                            larvae of the fly Lucilia cuprina feed on ovine
                            tissue and tissue fluids causing a cutaneous
                            myiasis associated with considerable host
                            morbidity and mortality. A candidate vaccine
                            antigen, peritrophin 95, was purified from the
                            peritrophic membrane, which lines the gut of
                            these larvae. Serum from sheep vaccinated with
                            peritrophin 95 inhibited growth of first-instar
                            L. cuprina larvae that fed on this serum. Growth
                            inhibition was probably caused by
                            antibody-mediated blockage of the normally
                            semipermeable peritrophic membrane and the
                            subsequent development of an impervious layer of
                            undefined composition on the gut lumen side of
                            the peritrophic membrane that restricted access
                            of nutrients to the larvae. The amino acid
                            sequence of peritrophin 95 was determined by
                            cloning the DNA complementary to its mRNA. The
                            deduced amino acid sequence codes for a secreted
                            protein containing a distinct Cys-rich domain of
                            317 amino acids followed by a mucin-like domain
                            of 139 amino acids. The Cys-rich domain may be
                            involved in binding chitin. This report
                            describes a novel immunological strategy for the
                            potential control of L. cuprina larvae that may
                            have general application to the control of other
                            insect pests.

                            PMID: 9256413, UI: 97404326

                       ------------------------------------------------------

                       5 : J Biol Chem 1996 Apr            Related Articles,
                       12;271(15):8925-35                    Books, Protein,
                                                        Nucleotide, LinkOut
                            [Image]
                            Characterization of a major peritrophic membrane
                            protein, peritrophin-44, from the larvae of
                            Lucilia cuprina. cDNA and deduced amino acid
                            sequences.

                            Elvin CM, Vuocolo T, Pearson RD, East IJ, Riding
                            GA, Eisemann CH, Tellam RL

                            CSIRO Division of Tropical Animal Production,
                            CSIRO Private Mail Bag 3, Indooroopilly, 4068,
                            Queensland, Australia.

                            The peritrophic membrane is a semi-permeable
                            chitinous matrix lining the gut of most insects
                            and is thought to have important roles in the
                            maintenance of insect gut structure,
                            facilitation of digestion, and protection from
                            invasion by microrganisms and parasites.
                            Proteins are integral components of this matrix,
                            although the structures and functions of these
                            proteins have not been characterized in any
                            detail. The peritrophic membrane from the larvae
                            of the fly Lucilia cuprina, the primary agent of
                            cutaneous myiasis in sheep, was shown to contain
                            six major integral peritrophic membrane
                            proteins. Two of these proteins, a 44-kDa
                            glycoprotein (peritrophin-44) and a 48-kDa
                            protein (peritrophin-48) together represent >70%
                            of the total mass of the integral peritrophic
                            membrane proteins. Peritrophin-44 was purified
                            and its complete amino acid sequence was
                            determined by cloning and sequencing the DNA
                            complementary to its mRNA. The deduced amino
                            acid sequence codes for a protein of 356 amino
                            acids containing an amino-terminal signal
                            sequence followed by five similar but
                            nonidentical domains, each of approximately 70
                            amino acids and characterized by a specific
                            register of 6 cysteines. One of these domains
                            was also present in the noncatalytic regions of
                            chitinases from Brugia malayi, Manduca sexta,
                            and Chelonus. Peritrophin-44 has a uniform
                            distribution throughout the larval peritrophic
                            membrane. Reverse transcriptase-polymerase chain
                            reaction detected the expression of
                            peritrophin-44 in all three larval instars but
                            only trace levels in adult L. cuprina. The
                            protein binds specifically to tri-N-acetyl
                            chitotriose and reacetylated chitosan in vitro.
                            It is concluded that the multiple cysteine-rich
                            domains in peritrophin-44 are responsible for
                            binding to chitin, the major constituent of
                            peritrophic membrane. Peritrophin-44 probably
                            has roles in the maintenance of peritrophic
                            membrane structure and in the determination of
                            the porosity of the peritrophic membrane. This
                            report represents the first characterization of
                            an insect peritrophic membrane protein.

                            PMID: 8621536, UI: 96224111

                       ------------------------------------------------------

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From owner-proteins@hgmp.mrc.ac.uk  Tue Jan 18 11:40:22 2000
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From: Lee Hunt <L.hunt@sheffield.ac.uk>
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Whats the best affinity matrix to use to bind a peptide of 12 amino
acids for antibody purification?



From owner-proteins@hgmp.mrc.ac.uk  Tue Jan 18 18:30:53 2000
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Subject: Re: pH question!
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PH is defined as 1/ - log (H ion )............

Vernon Drake wrote:

> Could someone tell me what the letters pH stand for?  Not the
> definetion or what pH is. I think it means the POWER of HYDROGEN but I
> am not quite sure.
> vdrake@wgn.net



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Read the question then answer the question.  


If you have read the previous postings in this thread then you would have
seen the answer.

Peter Pediaditakis

In article <3884B2BC.54C62879@biocomp.unl.edu>, Chris Larosa
<clarosa@biocomp.unl.edu> wrote:

> PH is defined as 1/ - log (H ion )............
> 
> Vernon Drake wrote:
> 
> > Could someone tell me what the letters pH stand for?  Not the
> > definetion or what pH is. I think it means the POWER of HYDROGE