From owner-protista@net.bio.net Sun Sep 13 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Yvan Lindekens" Newsgroups: bionet.mycology,bionet.parasitology,bionet.protista,sci.bio.botany,sci.bio.microbiology,sci.techniques.microscopy Subject: wanted: replacement for lactophenol- anilin blue stain Date: 14 Sep 1998 14:16:13 -0700 Organization: University of Ghent, Belgium Lines: 14 Sender: nospam@net.bio.net Approved: news@net.bio.net Distribution: world Message-ID: <01bddf55$65191a00$LocalHost@dialup.rug.ac.be> NNTP-Posting-Host: net.bio.net X-Newsreader: Microsoft Internet News 4.70.1155 Xref: biosci bionet.mycology:7297 bionet.parasitology:3486 bionet.protista:1179 Lactophenol is used as a mountant for all sorts of objects. Lactopenol-anilin blue and lactphenol-acid fuchsin are often used for the staining of fungi. Does anyone know of a substitute for these staining solutions: I want to get rid of the phenol... All suggestions are welcome. Please reply trough email: I'll make a summary for the group. Thanks! From owner-protista@net.bio.net Tue Sep 15 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Yvan Lindekens" Newsgroups: bionet.mycology,bionet.parasitology,bionet.protista,sci.bio.botany,sci.bio.microbiology,sci.techniques.microscopy Subject: wanted: replacement for lactophenol- anilin blue: summary of answers Date: 15 Sep 1998 22:04:35 -0700 Organization: University of Ghent, Belgium Lines: 58 Sender: nospam@net.bio.net Approved: news@net.bio.net Distribution: world Message-ID: <01bde107$11e0ae80$111fc19d@dialup.rug.ac.be> NNTP-Posting-Host: net.bio.net X-Newsreader: Microsoft Internet News 4.70.1155 Xref: biosci bionet.mycology:7303 bionet.parasitology:3489 bionet.protista:1180 "Lactophenol is used as ... Does anyone know of a substitute for these staining solutions: I want to get rid of the phenol..." Summary of answers: _____________________________________ From: jmhi@cybercom.net (Jeffrey C. May): "1% Acid fuchsin in lactic acid works fine. Don't use the phenol!" _____________________________________ From: boytrytis@peachnet.campus.mci.net (David Slomczynski): "I haven't found a replacement, yet. The problem is not neccessarily the phenol (there are certain over the counter medications that use more phenol - Chloraseptic and other - we injest it). I don't think I would worry so much about it - You normally use so little that is shouldn't affect anything. _____________________________________ From: dpfister@oeb.harvard.edu (Donald Pfister): "try lactic acid alone" _____________________________________ From: Helen TURNER I often use toluidine blue in aqeous solution (0.05% w/v or lower). This stains hyphal cytoplasm and spores quite nicely, and I've also used it to visualise the hyphae of fungal pathogens within (hand or cryotome) sectioned leaf and root material. Leaf material can be a bit of a problem, since hyphae stain purple and mesophyll cytplasm stains blue. Looks OK to the eye, but hard to distinguish in black-and-white photographs, if you're looking to publish without having to pay for inclusion of colour plates. I have also used aqueous aniline blue for staining fungi with some success. One other thing you might try, if you have access to a UV microscope - a number of fungi fluoresce quite nicely under UV illumination, especially if you add some dilute fluorescent brightener (e.g. from Sigma-Aldrich). Works a treat for showing pathogens growing over leaf surfaces, but not good for picking up hyphae inside host tissues. Hope this helps, ___________________________________________ Thanks to all! Yvan Lindekens. From owner-protista@net.bio.net Wed Sep 16 23:00:00 1998 Path: biosci!biosci!not-for-mail From: gordonr@cc.UManitoba.CA (Richard Gordon) Newsgroups: bionet.protista Subject: Re: live video? Date: 17 Sep 1998 08:07:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Approved: farmer@cellmate.cb.uga.edu Distribution: world Message-ID: <6tr8j4$49l@net.bio.net> NNTP-Posting-Host: net.bio.net Nina A. Allen and the late Robert D. Allen produced some beautiful movies. This may give a source: Allen, N. S. (1973). Endoplasmic Filaments in Nitella translucens [Film]. 1105 Truman Rd., Kansas City, MO 64106 USA: Calvin Communications. >Dear subscribers of micropal, >Could anyone tell me if there is an available video that shows zooplankton >and/or phytoplankton live. A friend of mine is looking for a live action >video to show his paleontology class. >Thanks in advance for any help you can provide. >Cindy Fisher >cfisher@wcupa.edu -------------------------------------------------------------------- Dr. Richard Gordon, Department of Radiology President, Canadian Society for Theoretical Biology University of Manitoba, Health Sciences Centre, Room GA248 820 Sherbrook Street, Winnipeg, MB R3A 1R9 Canada Phone: (204) 789-3828, Fax: (204) 787-2080, Home: (204) 589-0411 E-mail: GordonR@cc.UManitoba.ca From owner-protista@net.bio.net Wed Sep 16 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Fisher, Cynthia" Newsgroups: bionet.protista Subject: live video? Date: 17 Sep 1998 06:58:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Approved: farmer@cellmate.cb.uga.edu Distribution: world Message-ID: <6tr4hm$m1v@net.bio.net> NNTP-Posting-Host: net.bio.net Dear subscribers of micropal, Could anyone tell me if there is an available video that shows zooplankton and/or phytoplankton live. A friend of mine is looking for a live action video to show his paleontology class. Thanks in advance for any help you can provide. Cindy Fisher cfisher@wcupa.edu From owner-protista@net.bio.net Sun Sep 20 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Charles G. Sellers" Newsgroups: bionet.protista Subject: cryptomonad plastid isolation Date: 21 Sep 1998 04:59:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Approved: farmer@cellmate.cb.uga.edu Distribution: world Message-ID: <6u5f2g$4kv@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Colleagues, I am wondering if anyone has any experience (either sucessful or unsucessful) isolating intact chloroplasts or plastidial complexes from cryptomonads. I'd appreciate any advice and suggestions about how standard plant plastid isolation protocols can be suitably modified to acheive this end. (or also what I shouldn't try because it doesn't work) Sincerely, C. Grier Sellers Department of Biology University of Maryland College Park, MD 20742 - From owner-protista@net.bio.net Wed Sep 23 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Richard A. Snyder, Ph.D." Newsgroups: bionet.protista Subject: "sarcodine" phylogeny Date: 24 Sep 1998 05:49:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Approved: farmer@cellmate.cb.uga.edu Distribution: world Message-ID: <6udf44$5m9@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone have (or can point me towards) an rRNA based phylogenetic tree showing the position of silicoflagellates, foraminiferans, heliozoans and radialarians relative to other eukaryotes? Needed for Proto class. Thanks Richard A. Snyder, Ph.D. Center for Environmental Diagnostics and Bioremediation (CEDB) Biology Department University of West Florida 11,000 University Parkway Pensacola, FL 32514 http://www.uwf.edu/~rsnyder/ (850) 474-2806 FAX: -3130 From owner-protista@net.bio.net Mon Sep 28 23:00:00 1998 Path: biosci!biosci!not-for-mail From: pkugrens@lamar.colostate.edu (Paul Kugrens) Newsgroups: bionet.protista Subject: POST-DOC OPENING Date: 29 Sep 1998 04:57:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Approved: farmer@cellmate.cb.uga.edu Distribution: world Message-ID: <6uqhuu$c55@net.bio.net> NNTP-Posting-Host: net.bio.net Paul Falkowski asked me to post the following announcement. Paul Post-doctoral Research Associate: A post-doctoral research associate position is available 1 October at Rutgers University. The position is to study autocatalyzed cell death in unicellular algae. The applicant should be familiar with bsaic methods in biochemistry and molecular biology. Previous experience with proteases is highly desired. The position is funded for 2 years by NIH. Rutgers University is an equal opportunity employer. Applicants should send a cv, copies of recent research papers, and names of three references to: Professor Paul Falkowski, Environmental Biophysics and Molecular Biology Program, Institute of Marine and Coastal Science, Rutgers University, 71Dudley Road, New Brunswick, NJ 08901-8521. Thank you Paul Falkowski Environmental Biophysics and Molecular Ecology Program Institute of Marine and Coastal Sciences falko@ahab.Rutgers.EDU From owner-protista@net.bio.net Wed Sep 30 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Horizon Scientific Press Newsgroups: bionet.protista Subject: Molecular Biology of Protists: Call for Papers Date: 1 Oct 1998 05:06:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 59 Sender: daemon@net.bio.net Approved: farmer@cellmate.cb.uga.edu Distribution: world Message-ID: <6uvr85$s7i@net.bio.net> NNTP-Posting-Host: net.bio.net Editor-in-Chief: Milton H. Saier, San Diego Senior Editors: Peter Duerre, Ulm; Masayori Inouye, Piscataway; Takeshi Mizuno, Nagoya; George P.C. Salmond, Cambridge; Peter Reeves, Sydney The Journal of Molecular Microbiology and Biotechnology (JMMB) is currently accepting manuscripts for publication in the first issue. The journal publishes high-quality primary research papers, in English, in all areas of molecular microbiology and molecular biotechnology, and in addition contains some timely reviews and written symposia on specific topics. JMMB publishes original work on the molecular biology or molecular biotechnology of microorganisms (including bacteria, viruses, and eukaryotic microorganisms). JMMB is an international peer-reviewed journal. The first issue will be published March 1999. Manuscripts to be considered for publication in the Journal of Molecular Microbiology and Biotechnology should be sent immediately to any one of the Senior Editors. *** Milton H. Saier *** Department of Biology, University of California at San Diego, La Jolla, CA 92093-0116, USA *** Peter Duerre *** Angewandte Mikrobiologie, und Mykologie, Universitaet Ulm, D-89069 Ulm, Germany *** Masayori Inouye *** Rutgers State Univ, Robert Wood Johnson Med Sch, Dept Biochem, Piscataway, NJ 08854, USA *** Takeshi Mizuno *** Nagoya University, School Agriculture, Mol Microbiol Lab, Chikusa Ku, Nagoya, Aichi 464, Japan *** George P.C. Salmond *** Dept. of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW, UK *** Peter Reeves *** Department of Microbiology (G08), University of Sydney, Sydney, NSW, 2006 , Australia Full details (including Instructions for Authors) may be found at: http://www.horizonpress.com/jmmb or can be obtained from the publisher: Horizon Scientific Press PO Box 1 Wymondham Norfolk NR18 0EH mail@horizonpress.com Journal of Molecular Microbiology and Biotechnology ISSN 1464-1801 KEY FEATURES: * Rapid publication * No page charges * Free offprints * Free sample copy * Expert and rigorous peer review