From owner-pseudomonas@net.bio.net Fri Feb 02 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!uwm.edu!msunews!netnews.upenn.edu!mail2.sas.upenn.edu!pnealen From: pnealen@mail2.sas.upenn.edu (Paul M. Nealen) Newsgroups: bionet.microbiology,sci.bio.microbiology,bionet.organisms.pseudomonas Subject: Pseudomonas transformation Date: 3 Feb 1996 20:34:08 GMT Organization: University of Pennsylvania Lines: 18 Message-ID: <4f0gs0$6no@netnews.upenn.edu> NNTP-Posting-Host: mail2.sas.upenn.edu X-Newsreader: TIN [version 1.2 PL2-upenn1.3] Xref: biosci bionet.microbiology:4821 sci.bio.microbiology:2571 bionet.organisms.pseudomonas:58 I am seeking information on transformation of Pseudomonas - I know that 37 degrees and Ca++ are used, as is 37 degrees and Mg++. Have any other temperature/ion combinations been used? Thanks - Paul -- ------------------------------------------------------------------------------ Paul M. Nealen tel: (215) 898-6559 Biology Department fax: (215) 898-8780 University of Pennsylvania Philadelphia PA 19104-6018 pnealen@mail.sas.upenn.edu ------------------------------------------------------------------------------ From owner-pseudomonas@net.bio.net Sat Feb 03 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.tamu.edu!news.utdallas.edu!not-for-mail From: corboy@utdallas.edu Newsgroups: bionet.microbiology,sci.bio.microbiology,bionet.organisms.pseudomonas Subject: Re: Pseudomonas transformation Followup-To: bionet.microbiology,sci.bio.microbiology,bionet.organisms.pseudomonas Date: 3 Feb 1996 21:47:28 -0600 Organization: The University of Texas at Dallas Lines: 28 Message-ID: <4f1a8g$6v7@utdallas.edu> References: <4f0gs0$6no@netnews.upenn.edu> NNTP-Posting-Host: infoserv.utdallas.edu NNTP-Posting-User: 10308 X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.microbiology:4825 sci.bio.microbiology:2573 bionet.organisms.pseudomonas:59 Paul M. Nealen (pnealen@mail2.sas.upenn.edu) wrote: > I am seeking information on transformation of Pseudomonas - I know that > 37 degrees and Ca++ are used, as is 37 degrees and Mg++. Have any other > temperature/ion combinations been used? > Thanks - > Paul > -- I'm assuming by 37 deg you mean the heat shock. If so, then in the past we used cells treated with Mg and heat shocked at 42 deg just like E coli. This worked, but since all we were asking for was uptake of supercoiled plasmids previously constructed and verified in E coli, efficiency was not a problem. However, we now use electroporation following the protocol in the BRL handbook that comes with the voltage booster. This works very well, is quicker, and the cells are easier to make. A caution, tho, I routinely use 20ul of EP competent cells + 0.1ug or so of plasmid, and if I plate more than 1ul of the 20 I get confluent plates. Hope this helps, but if you don't have an electroporator, the Mg and 42 deg works well enough as long as you are not trying transformation say ligation reactions. Michael J. Corboy University of Texas at Dallas Molecular and Cell Biology From owner-pseudomonas@net.bio.net Sun Feb 04 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!univ-lyon1.fr!in2p3.fr!swidir.switch.ch!cisun2000.unil.ch!macbb2212a.unil.ch!user From: Stephan.Heeb@Lbm.unil.ch (Stephan Heeb) Newsgroups: bionet.microbiology,sci.bio.microbiology,bionet.organisms.pseudomonas Subject: Re: Pseudomonas transformation Date: Mon, 05 Feb 1996 15:48:57 +0200 Organization: University of Lausanne CH (Switzerland) Lines: 71 Message-ID: References: <4f0gs0$6no@netnews.upenn.edu> NNTP-Posting-Host: macbb2212a.unil.ch Xref: biosci bionet.microbiology:4842 sci.bio.microbiology:2589 bionet.organisms.pseudomonas:60 In article <4f0gs0$6no@netnews.upenn.edu>, pnealen@mail2.sas.upenn.edu (Paul M. Nealen) wrote: > I am seeking information on transformation of Pseudomonas - I know that > 37 degrees and Ca++ are used, as is 37 degrees and Mg++. Have any other > temperature/ion combinations been used? > > Thanks - Here you have the protocol we are using for routine tranformation of Pseudomonas fluorescens strain CHA0. Transformation of this strain is not possible with electroporation (high frequencies of mutations were observed) and the temperatures should not exceed 35°C (the strain won't grow at 37°C). This method is a modification of the stadard protocol described for E. coli in "Molecular Clonig, A Laboratory Manual"; Sambrook et al. 1990) Good luck, Stephan Heeb Lab. Biol. Micr. e-mail: Stephan.Heeb@LBM.unil.ch Universite de Lausanne 1015 Lausanne Switzerland =================== LBM PROTOCOLS ------------- PREPARATION OF FROZEN STOCKS OF COMPETENT CELLS AND TRANSFORMATION OF PSEUDOMONAS FLUORESCENS CHA0 Dilute 300 ul of a fresh overnight culture into 30 ml NYB and incubate with shaking at 35°C (to prevent restriction) until OD600 = 1.8-2.0 Centrifuge 5 min. at 5'000 rpm 4°C in a Corex tube and resuspend cells in 7.5 ml of chilled 0.1 M calcium chloride using a Pasteur pipette. Leave on ice for 30 min. Centrifuge 5 min. at 5'000 rpm 4°C and resuspend cells in 1.5 ml of chilled 0.1 M calcium chloride using a Pasteur pipette. * Add 50 ul of DMSO, mix gently by swirling, and store the suspension on ice for 15 minutes. * Add an additional 50 ul of DMSO to the suspension. Mix gently by swirling, and then return the suspension to an ice bath. * Working quickly, dispense 100 µl aliquots of the suspensions into chilled, sterile Eppendorf tubes. Snap-freeze the competent cells by immersing the thightly closed tubes in liquid nitrogen or in a dry ice/ethanol bath. Store the tubes at -70°C until needed. * When needed, thaw the cells in an ice bath. Store the cells on ice for 10 minutes. Mix 100-500 ng plasmid DNA with 100 ul of competent cells. Leave on ice for 30 min. Heat pulse at 42-43°C for 2 min. Add prewarmed (35°C) NYB to complete 1 ml and incubate shaken at 35°C for 1 to 3 hrs depending on the plasmid copy number (less time is required for high copy number plasmids). Plate on selective media. From owner-pseudomonas@net.bio.net Mon Feb 05 22:00:00 1996 Newsgroups: bionet.organisms.pseudomonas Path: biosci!rutgers!rockyd!news.sprintlink.net!news.bluesky.net!solaris.cc.vt.edu!newsfeed.internetmci.com!howland.reston.ans.net!Germany.EU.net!ieunet!news.tcd.ie!mac03.arches.pclab.tcd.ie!user From: kpryan@tcd.ie (Sean Ryan) Subject: Irish First Class Hons Parasitology Grad. for Ph.d? Message-ID: Sender: usenet@news.tcd.ie (TCD News System ) Organization: Trinity College, Dublin 2 Date: Tue, 6 Feb 1996 09:37:45 GMT Lines: 13 I am a graduate of Trinity College Dublin with a first class honours degree in zoology and a specialisation in Zoology and am now looking for a suitable Ph.D. project in the epidemiology/pathology of a parasite of medical or veterinary importance. If anybody has a position for a Ph.D. student with excellent qualifications or who knows of somebody who might I would appreciate a response to kpryan@tcd.ie Response from Europe/Australia especially welcome, all replies will be acknowledged Thanks Sean Ryan From owner-pseudomonas@net.bio.net Sun Feb 11 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!tank.news.pipex.net!pipex!lade.news.pipex.net!pipex!ggr.co.uk!ussun2n.glaxo.com!concert!bigblue.oit.unc.edu!not-for-mail From: David Parsons Newsgroups: bionet.organisms.pseudomonas Subject: Methods to detect Pseudomonas aeruginosa in histological slides? Date: 12 Feb 1996 20:38:06 GMT Organization: UNC - School of Medicine, CF/Pulmonary Research& Treatment Center Lines: 25 Message-ID: <4fo8fe$118j@bigblue.oit.unc.edu> NNTP-Posting-Host: pc295a4021a.med.unc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) Greetings again, I need to quantify, or at least identify the presence of, Pseudomonas in 10% neutral bufferred fomalin fixed histological slides (standard light microscopy). I am taking sections from the decalcified (formic acid in formalin) mouse nose; the usual bacteriological grind-and plate-approach is not workable due to frequent overgrowths and inabilitiy to reliable define where exactly the bacteria came from in the nose. I have used the Brown-Hopps stain, and that is , but hard to differentiate bugs from all the other nasal junk in the sections of the nose, and quantitation would be a nightmare; I wonder if there is a more specific stain - and antibody, a flourescent method, or anything else, that I could use in these sections. Or can I tag these Pseudomonas before they go into the nose with something that will last over several days? Classical solid science, as well 'as wild-and-crazy' suggestions welcome. Thanks pseudo-persons! Dr David Parsons UNC@ Chapel Hill Dept of Medicine From owner-pseudomonas@net.bio.net Tue Feb 13 22:00:00 1996 Path: biosci!daresbury!yama.mcc.ac.uk!peer-news.britain.eu.net!tank.news.pipex.net!pipex!news.sprintlink.net!news.gte.net!news From: max@gte.net Newsgroups: bionet.organisms.pseudomonas Subject: Pseudo in water--illness?? Date: 13 Feb 1996 23:50:58 GMT Organization: GTE Intelligent Network Services, GTE INS Lines: 3 Message-ID: <4fr852$m66@duey.gte.net> NNTP-Posting-Host: dfw69084.gte.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22KIT (Windows; U; 16bit) What types of Pesudomonads if any would cause illness when injested (via drinking water)?? From owner-pseudomonas@net.bio.net Tue Feb 13 22:00:00 1996 Newsgroups: bionet.organisms.pseudomonas Path: biosci!lhc.nlm.nih.gov!crick.sura.net!lamarck.sura.net!newsfeed.internetmci.com!howland.reston.ans.net!Germany.EU.net!ieunet!news.tcd.ie!mac01.arches.pclab.tcd.ie!user From: kpryan@tcd.ie (Sean Ryan) Subject: Irish First Class Hons Parasitology Grad for Ph.D. Message-ID: Sender: usenet@news.tcd.ie (TCD News System ) Organization: Trinity College, Dublin 2 Date: Wed, 14 Feb 1996 10:06:20 GMT Lines: 12 I am a graduate of zoology from Trinity College Dublin with a first class hons degree and a specialisation in parasitology and am now looking for a suitable Ph.D. program on the epidemiology/pathology of (a) parasite(s) of medical/veterinary importance. If any body has a place for a Ph.D student or knows of someone who might I would apreciate an e-mail to kpryan@tcd.ie Thanks Sean Ryan replies from Australia/Europe especially welcome From owner-pseudomonas@net.bio.net Thu Feb 15 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.organisms.pseudomonas Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 16 Feb 1996 02:00:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199602161000.CAA29115@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-pseudomonas@net.bio.net Fri Feb 16 22:00:00 1996 Path: biosci!news.Stanford.EDU!nntp-hub2.barrnet.net!news1.digital.com!decwrl!nntp.crl.com!acara.snsnet.net!HiWAAY.net!news.sprintlink.net!tank.news.pipex.net!pipex!swrinde!cs.utexas.edu!news.unt.edu!news From: farinha@cas1.unt.edu (Mark Farinha) Newsgroups: bionet.organisms.pseudomonas Subject: Re: Pseudo in water--illness?? Date: 16 Feb 1996 20:03:17 GMT Organization: University of North Texas Lines: 6 Message-ID: <4g2nu5$j10@hermes.acs.unt.edu> References: <4fr852$m66@duey.gte.net> NNTP-Posting-Host: farinha.biol.unt.edu X-Newsreader: WinVN 0.92.1 In article <4fr852$m66@duey.gte.net>, max@gte.net says: > >What types of Pesudomonads if any would cause illness when injested (via drinking >water)?? > No bacteria like to be made fun of! From owner-pseudomonas@net.bio.net Wed Feb 21 22:00:00 1996 Path: biosci!daresbury!keele!peer-news.britain.eu.net!tank.news.pipex.net!pipex!newsfeed.internetmci.com!in1.uu.net!news.u.washington.edu!carson.u.washington.edu!tmcmille From: Tia McMillen Newsgroups: bionet.organisms.pseudomonas Subject: Re: Pseudo in water--illness?? Date: Thu, 22 Feb 1996 09:58:20 -0800 Organization: University of Washington Lines: 15 Message-ID: References: <4fr852$m66@duey.gte.net> NNTP-Posting-Host: carson.u.washington.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <4fr852$m66@duey.gte.net> I don't know much about pseudomonas, but in 1988 I was taken to the hospital and diagnosed with 2, 8oz abcesses within my abdominal cavity. When a biopsy was taken, pseudomonas was the infection. They don't know what caused any of it, but assumed my appendix had ruptured, poisened me, then healed. I was sick as a dog for 4 weeks, but still have my appendix. I don't think I injested it, though. -Tia On 13 Feb 1996 max@gte.net wrote: > What types of Pesudomonads if any would cause illness when injested (via drinking > water)?? > > > From owner-pseudomonas@net.bio.net Sun Feb 25 22:00:00 1996 Path: biosci!bloom-beacon.mit.edu!newsfeed.internetmci.com!usenet.eel.ufl.edu!usenet.cis.ufl.edu!usenet.ufl.edu!usenet From: bwr@icbr.ifas.ufl.edu (Bruce W. Ritchings) Newsgroups: bionet.organisms.pseudomonas Subject: Re: Methods to detect Pseudomonas aeruginosa in histological slides? Date: Mon, 26 Feb 1996 22:14:01 GMT Organization: University of Florida Lines: 42 Message-ID: <4gt0lq$5q0@no-names.nerdc.ufl.edu> References: <4fo8fe$118j@bigblue.oit.unc.edu> NNTP-Posting-Host: bruce.med.ufl.edu X-Newsreader: Forte Free Agent 1.0.82 David Parsons wrote: >Greetings again, >I need to quantify, or at least identify the presence of, Pseudomonas in 10% neutral >bufferred fomalin fixed histological slides (standard light microscopy). I am >taking sections from the decalcified (formic acid in formalin) mouse nose; the usual >bacteriological grind-and plate-approach is not workable due to frequent overgrowths >and inabilitiy to reliable define where exactly the bacteria came from in the nose. > >I have used the Brown-Hopps stain, and that is , but hard to differentiate >bugs from all the other nasal junk in the sections of the nose, and quantitation >would be a nightmare; I wonder if there is a more specific stain - and antibody, a >flourescent method, or anything else, that I could use in these sections. Or can I >tag these Pseudomonas before they go into the nose with something that will last >over several days? >Classical solid science, as well 'as wild-and-crazy' suggestions welcome. >Thanks pseudo-persons! >Dr David Parsons >UNC@ Chapel Hill >Dept of Medicine Hello David, So that's what we are, not real persons but pseudo-ones!? Nevermind, put the following in the "wild and crazy" list of suggestions to solve your problem: Our flow cytometry core lab at the University of Florida is doing alot with GFP (and it's red-shift variant, RSGFP), and they report that it is "stable after fixation with formaldehyde." This MAY mean that it'll be stable in your 10% neutral buffered formalin--worth investigating, at least. You'd have to put the gene for GFP into your Pseudomonas, I'd assume on a plasmid, but you might be able to get a stable integration in the chromosome. In either case, your Pseudomonas should light up beautifully, assuming you can excite them (can this be done on a slide?) at 488 nm and detected at 533 nm. Like I said, wild and crazy, but if it works, it would certainly light up your results! Best of luck, Bruce Ritchings, University of Florida From owner-pseudomonas@net.bio.net Sun Feb 25 22:00:00 1996 Newsgroups: bionet.organisms.pseudomonas Path: biosci!agate!ihnp4.ucsd.edu!swrinde!newsfeed.internetmci.com!in1.uu.net!news2.new-york.net!not-for-mail From: Steve Grenard Subject: Re: Pseudo in water--illness?? Content-Type: text/plain; charset=us-ascii X-Nntp-Posting-User: (Unauthenticated) Content-Transfer-Encoding: 7bit Organization: HerpMed Message-ID: References: <4fr852$m66@duey.gte.net> X-Mailer: Mozilla 1.2 (Windows; U; 16bit) Mime-Version: 1.0 X-Trace: 825290472/2360 X-Nntp-Posting-Host: herpmed.com Date: Sun, 25 Feb 1996 23:21:15 GMT Lines: 15 Pseudomonas aeruginsa. -- ================================================= Steve Grenard e-mail: grenard@herpmed.com POB 40825 - Staten Island, NY 10304-0825 Tel/Fax: 1-718-447-6144 Web: http://www.herpmed.com/ Resp: http://www.xmission.com/~gastown/herpmed/respi.htm ================================================= From owner-pseudomonas@net.bio.net Sun Feb 25 22:00:00 1996 Path: biosci!bloom-beacon.mit.edu!newsfeed.internetmci.com!usenet.eel.ufl.edu!usenet.cis.ufl.edu!usenet.ufl.edu!usenet From: bwr@icbr.ifas.ufl.edu (Bruce W. Ritchings) Newsgroups: bionet.organisms.pseudomonas Subject: Re: Pseudo in water--illness?? Date: Mon, 26 Feb 1996 22:26:44 GMT Organization: University of Florida Lines: 24 Message-ID: <4gt1dl$6ib@no-names.nerdc.ufl.edu> References: <4fr852$m66@duey.gte.net> NNTP-Posting-Host: bruce.med.ufl.edu X-Newsreader: Forte Free Agent 1.0.82 Tia McMillen wrote: >I don't know much about pseudomonas, but in 1988 I was taken to the >hospital and diagnosed with 2, 8oz abcesses within my abdominal cavity. >When a biopsy was taken, pseudomonas was the infection. They don't know >what caused any of it, but assumed my appendix had ruptured, poisened me, >then healed. I was sick as a dog for 4 weeks, but still have my >appendix. I don't think I injested it, though. >-Tia >On 13 Feb 1996 max@gte.net wrote: >> What types of Pesudomonads if any would cause illness when injested (via drinking >> water)?? >> >> >> Hello Tia, My boss, Dr. Ramphal at University of Florida, says that there is no proof that any of the Pseudomonads cause infection when injested, neither diarrhea nor invasive infections. However, he says, Aeromonads are a different story--they may cause such infections. Sincerely, Bruce Ritchings From owner-pseudomonas@net.bio.net Tue Feb 27 22:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!carbon!night.primate.wisc.edu!sdd.hp.com!swrinde!tank.news.pipex.net!pipex!news.uoregon.edu!news.orst.edu!news From: chend@ucs.orst.edu (Don Chen) Newsgroups: bionet.organisms.pseudomonas Subject: Ps fluorescens biovars-2nd try Date: Tue, 27 Feb 1996 06:43:16 GMT Organization: Oregon State University/USDA-ARS-NFSPRC Lines: 25 Message-ID: <4h0tgg$ko7@news.orst.edu> NNTP-Posting-Host: slip184.ucs.orst.edu X-Newsreader: Forte Free Agent 1.0.82 I'm reposting this because I don't know if the first attempt made it through. Hi: I have read that there are five biovars of Ps. fluorescens, but they are not described. Does anyone what are the type strains and where they can be obtained (eg ATCC?)? Is there specific references for these strains which are published and available. I wish to use them as reference to soil pseudomonads which I am trying to characterize. Thanks for any help you can offer. Don ********************************************************* Don Chen * Standard disclaimers apply here. USDA-ARS-NFSPRC * 3450 SW Campus Way * Corvallis, OR 97331 * * 503-750-8721 * ********************************************************