From owner-pseudomonas@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!biosci!not-for-mail From: Sean Ryan Newsgroups: bionet.parasitology,bionet.general,bionet.jobs.wanted,bionet.organisms.pseudomonas,bionet.organisms.schistosoma Subject: Irish First Class Hons. Parasitology Grad. for Ph.D. Date: 7 Aug 1996 17:34:21 -0700 Organization: Trinity College, Dublin, Ireland Lines: 13 Sender: biohelp@net.bio.net Distribution: world Message-ID: <4u9n7r$b9j@web3.tcd.ie> NNTP-Posting-Host: net.bio.net Xref: biosci bionet.parasitology:1702 bionet.general:22919 bionet.jobs.wanted:7406 bionet.organisms.pseudomonas:143 bionet.organisms.schistosoma:150 I am a graduate of zoology from Trinity College Dublin with a first class hons degree and a specialisation in Parasitology and am now looking for a suitable Ph.D. subject involving the epidemiology/pathology of a parasite(s) of medical/veterinary importance. If anybody has a place for a Ph.D. student or knows of someone who has, I would appreciate an e-mail to kpryan@tcd.ie thanks..Sean Ryan ps replies from Australia/Europe especially welcome. From owner-pseudomonas@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!gondor!newsfeeder.sdsu.edu!news.sgi.com!swrinde!cs.utexas.edu!howland.erols.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in3.uu.net!EU.net!usenet2.news.uk.psi.net!uknet!usenet1.news.uk.psi.net!uknet!uknet!strath-cs!dcl-cs!bath.ac.uk!aber!drh92 From: drh92@aber.ac.uk (DANIEL ROBERT HOLDSWORTH) Newsgroups: bionet.parasitology,bionet.general,bionet.jobs.wanted,bionet.organisms.pseudomonas,bionet.organisms.schistosoma Subject: Re: Irish First Class Hons. Parasitology Grad. for Ph.D. Date: 8 Aug 1996 14:09:03 +0100 Organization: The Knights who say "NI!" Lines: 7 Distribution: world Message-ID: <4ucotf$eo0@osfb.aber.ac.uk> References: <4u9n7r$b9j@web3.tcd.ie> Reply-To: drh92@aber.ac.uk (DANIEL ROBERT HOLDSWORTH) NNTP-Posting-Host: osfb.aber.ac.uk X-Newsreader: mxrn 6.18-9 Xref: biosci bionet.parasitology:1704 bionet.general:22931 bionet.jobs.wanted:7417 bionet.organisms.pseudomonas:144 bionet.organisms.schistosoma:151 Yes, we know. You've posted to this newsgroup umpteen times. Give it a rest for a while, can't you? Dan H. From owner-pseudomonas@net.bio.net Thu Aug 08 23:00:00 1996 Path: biosci!agate!howland.erols.net!news1.erols.com!hunter.premier.net!news-res.gsl.net!news.gsl.net!usenet.eel.ufl.edu!usenet.nerdc.ufl.edu!usenet.ufl.edu!usenet From: bwr@icbr.ifas.ufl.edu (Bruce W. Ritchings) Newsgroups: bionet.organisms.pseudomonas Subject: gene fusion vectors for Pseudomonas Date: Fri, 09 Aug 1996 22:42:10 GMT Organization: University of Florida Lines: 4 Message-ID: <320bbdcc.12855965@usenet.ufl.edu> NNTP-Posting-Host: bruce.med.ufl.edu X-Newsreader: Forte Agent .99c/16.141 Hello Pseudomoners, Does anyone have any good gene fusion expression vectors for Pseudomonas aeruginosa, similar to the pET or pMAL vectors for E. coli? Any help greatly appreciated, Sincerly, Bruce Ritchings From owner-pseudomonas@net.bio.net Sun Aug 11 23:00:00 1996 Path: biosci!PSU.EDU!jmc21 From: jmc21@PSU.EDU (Jonna M Coombs) Newsgroups: bionet.organisms.pseudomonas Subject: Cold-temperature bugs... Date: 12 Aug 1996 12:00:04 -0700 Organization: Pennsylvania State University Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <320F7F06.2433@psu.edu> NNTP-Posting-Host: net.bio.net Greetings, I work in a lab that studies psychrophillic bacteria. I know that there are some species of Pseudomonas that are tolerant of low temperatures (ie fluorescens or alcaligenes), and I was wondering if anyone knew of strains of these organisms that are known for their ability to grow well at 4 or 10 degrees C. Most of the information that I have been able to find has been in older articles, where the researchers use temperature (the ability to grow at 4 degrees C)to characterize the strain. There seem to be very few studies out there where people have taken a look at growth rates. If anyone knows of a strain that does grow rapidly at low temperature, or has any suggestions of where I might look, the input would be greatly appreciated! Jonna Coombs Penn State University jmc21@psu.edu From owner-pseudomonas@net.bio.net Mon Aug 12 23:00:00 1996 Path: biosci!MHUB1.TC.UMN.EDU!Jacks052 From: Jacks052@MHUB1.TC.UMN.EDU (John Kurt Jackson) Newsgroups: bionet.organisms.pseudomonas Subject: Flocculation; how do I avoid it? Date: 12 Aug 1996 20:38:06 -0700 Organization: University of Minnesota Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <320ff8af61a9005@mhub1.tc.umn.edu> NNTP-Posting-Host: net.bio.net My bacteria flocculate whenever I try to do that horrible thing, grow them. This makes taking OD readings very difficult, nay, impossible. I know this must be a common problem in bioreactors; is there a simple solution, e.g. adding some chemical or adjusting ion concentrations? I would appreciate advice from anyone who has also faced this problem... (note: my experiment requires N- minimal media; the one I'm using has a strong phosphate buffer, all minerals, and a carbon source). From owner-pseudomonas@net.bio.net Thu Aug 15 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.organisms.pseudomonas Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 16 Aug 1996 02:00:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199608160900.CAA27645@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. 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For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-pseudomonas@net.bio.net Sun Aug 18 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!imci3!newsfeed.internetmci.com!in3.uu.net!EU.net!Portugal.EU.net!news.rccn.net!scsing.switch.ch!elna.ethz.ch!usenet From: Michael Kertesz Newsgroups: bionet.organisms.pseudomonas Subject: Re: Flocculation; how do I avoid it? Date: Mon, 19 Aug 1996 17:44:25 +0200 Organization: Microbiology ETHZ Lines: 29 Message-ID: <32188BD9.4373@micro.biol.ethz.ch> References: <320ff8af61a9005@mhub1.tc.umn.edu> NNTP-Posting-Host: b22-pro486-1.ethz.ch Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) John Kurt Jackson wrote: > > My bacteria flocculate whenever I try to do that horrible thing, grow > them. This makes taking OD readings very difficult, nay, impossible. I > know this must be a common problem in bioreactors; is there a simple > solution, e.g. adding some chemical or adjusting ion concentrations? I > would appreciate advice from anyone who has also faced this problem... > (note: my experiment requires N- minimal media; the one I'm using has a > strong phosphate buffer, all minerals, and a carbon source). I solved this problem in a sulfur-limited system by adding amino acids to my medium (i. e. by increasing the growth rate). I can see that that's not practical for you, but perhaps you could speed up growth some other way - shaking harder? Alternatively, it was suggested to me at the time that I should add 0.1% (v/v) Tween 80 to the medium - it seems the lactococcus people do this routinely to stop clumping. hope that's some help, -- Michael Kertesz ETH-Microbiology, ETH-Zentrum/LFV, CH-8092 Zurich, Switzerland tel: +41-1-632 3357 fax: +41-1-632 1148 e-mail: kertesz@micro.biol.ethz.ch From owner-pseudomonas@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!imci3!newsfeed.internetmci.com!news.stealth.net!nntp04.primenet.com!nntp.primenet.com!netcom.com!netcom.net.uk!ix.netcom.com!news From: krokos@ix.netcom.com (Christopher Krokos *USA*) Newsgroups: bionet.organisms.pseudomonas Subject: Q: Parasite in a fish meat ? Date: Thu, 22 Aug 1996 10:00:28 GMT Organization: Netcom Lines: 23 Message-ID: <321c2fa6.1290023@nntp.ix.netcom.com> NNTP-Posting-Host: nhv-ct1-02.ix.netcom.com X-NETCOM-Date: Thu Aug 22 3:05:22 AM PDT 1996 X-Newsreader: Forte Agent .99c/16.141 My family usually shops for grocery at STOP&SHOP in Connecticut. Yesterday (8/21) we prepared a fish for a dinner. Before serving it, a fish was well fried. What I have found and saw in the fish meat I couldn't believe my eyes. About one (1") inch long, very thin worm. I am almost sure, that it is a fish PARASITE. I removed a parasite from the meet and placed in a jar with water for a luck of formaline. I keep the worm cold in the refrigerator at +4C degrees. I would like to know which federal agencies or laboratories regulate the quality of food sold to the public, and where the parasite can be identified by scientist or biologist. Thank you for any help and suggestion, Christopher A Krokos E-mail: krokos@ix.netcom.com From owner-pseudomonas@net.bio.net Wed Aug 21 23:00:00 1996 Path: biosci!uoguelph.ca!derrampa From: derrampa@uoguelph.ca (Deena Errampalli) Newsgroups: bionet.organisms.pseudomonas Subject: Bioremediation Resources on the Internet Date: 22 Aug 1996 11:10:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 65 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Members of Pseudomonas news group, I am pleased to announce a new WWW site dedicated to bioremediation resources on the Internet. This site is being developed as a collaborative effort of the Department of Environmental Biology, University of Guelph, the Groundwater Remediation Project, National Water Research Institute, Environment Canada, and GZA GeoEnvironmental, Inc. The site currently includes an archive of the Bioremediation mailing list, a bioremediation LinksBase, and a list of Internet services with a focus on bioremediation. The archive of the Bioremediation mailing list was developed with the permission and assistance of GZA GeoEnvironmental, Inc., the host of Biogroup. The archive is updated daily, and is indexed and searchable, making the retrieval of past messages quick and convenient. The bioremediation LinksBase is a managed database of 247 WWW documents relevant to bioremediation. A search capability allows you to locate documents containing arbitrary search terms, and then follow hyperlinks back to the source WWW site. The LinkBase will be updated regularly to reflect new and revised documents; a form is provided so that you can directly submit URLs for inclusion in the LinksBase. You can access "Bioremediation Resources on the Internet" at http://gwrp.cciw.ca/internet/bioremediation/ We hope that you have an opportunity to visit this site. Please send your comments regarding the site to the addresses listed below. Deena Errampalli Department of Environmental Biology University of Guelph Guelph, Ontario, N1G 2W1, Canada derrampa@uoguelph.ca and Andrew R. Piggott National Water Research Institute 867 Lakeshore Rd. Burlington, Ontario, L7R 4A6, Canada andrew.piggott@cciw.ca ------------------------------------------------------------------- For subscription and other administrative information on the BioGroup, please send a message to rschaffner@gzea.com or webmaster@gzea.com, or visit our home page at http://www.gzea.com ------------------------------------------------------------------- NOTE: This is an unmoderated mailing list. It is being hosted by GZA GeoEnvironmental, Inc. as a service to the bioremediation community. GZA is not responsible for the content of postings made to this mailing list.