From owner-rapd@net.bio.net Sun Jun 06 23:00:00 1993 Path: biosci!edvz.uni-graz.ada.at!VANSTAAD From: VANSTAAD@edvz.uni-graz.ada.at ("Moira J. van Staaden") Newsgroups: bionet.molbio.rapd Subject: Operon address Message-ID: <156*.S=VANSTAAD.O=edvz.PRMD=uni-graz.ADMD=ada.C=at.@MHS> Date: 7 Jun 93 21:57:24 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 4 I'd appreciate if someone could tell me the address, fax etc for Operon Technologies, Ca. Thanks, MvS From owner-rapd@net.bio.net Sun Jun 06 23:00:00 1993 Path: biosci!uwm.edu!spool.mu.edu!howland.reston.ans.net!ux1.cso.uiuc.edu!sdd.hp.com!news.cs.indiana.edu!nstn.ns.ca!dragon.acadiau.ca!ace.acadiau.ca!msnyder From: msnyder@ace.acadiau.ca (MARLENE SNYDER) Newsgroups: bionet.molbio.rapd Subject: Question: Use of Ultra-pure agarose necessary.....? Message-ID: Date: 7 Jun 93 17:21:39 GMT Sender: news@dragon.acadiau.ca Organization: Acadia University Lines: 8 Nntp-Posting-Host: 131.162.69.36 I am curious: We are about to start attempting some RAPD techniques here. However, all we are intersted in is the banding patterns on the agarose (and NOT interested in purification of bands after separation). Thus, is it necessary to use Ultra pure (and extra expensive) Agarose? Or can we get away with using standard lab grade agarose? Thanks! From owner-rapd@net.bio.net Mon Jun 07 23:00:00 1993 Path: biosci!daresbury!daresbury!news From: UDBR150@OAK.CC.KCL.AC.UK Newsgroups: bionet.molbio.rapd Subject: Use of ultra pure Agarose Message-ID: <1993Jun8.115632.27397@gserv1.dl.ac.uk> Date: 8 Jun 93 11:12:00 GMT Sender: list-admin@daresbury.ac.uk Distribution: bionet Lines: 7 Original-To: RAPD@UK.AC.DARESBURY In reply to M.Snyders question about the use of ultra pure Agarose for the sepparation of RAPD bands.......We have always used standard grade agarose for the visualisation of the amplification products, although a higher grade is better for the purification of bands after separation. hope this of use to you David From owner-rapd@net.bio.net Tue Jun 08 23:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!uknet!pipex!uunet!psgrain!ee.und.ac.za!ford.ee.up.ac.za!hippo.ru.ac.za!mirk From: mirk@hippo.ru.ac.za (Prof R Kirby) Newsgroups: bionet.molbio.rapd Subject: Re: Question: Use of Ultra-pure agarose necessary.....? Message-ID: <1993Jun9.083051.18641@hippo.ru.ac.za> Date: 9 Jun 93 08:30:51 GMT References: Organization: Rhodes University, Grahamstown, South Africa Lines: 18 In msnyder@ace.acadiau.ca (MARLENE SNYDER) writes: >I am curious: We are about to start attempting some RAPD techniques here. >However, all we are intersted in is the banding patterns on the agarose (and >NOT interested in purification of bands after separation). Thus, is it >necessary to use Ultra pure (and extra expensive) Agarose? Or can we get >away with using standard lab grade agarose? Try using acrylamide and silver staining, it provides a greater amount of information per PCR and is just as good as agarose. Professor Ralph Kirby Rhodes University Grahamstown -- Prof Ralph Kirby - Department of Microbiology - Rhodes University Internet: mirk@hippo.ru.ac.za Telephone: (0461) 22023 xt 441 From owner-rapd@net.bio.net Tue Jun 08 23:00:00 1993 Path: biosci!enterpoop.mit.edu!gatech!concert!news-feed-1.peachnet.edu!emory!swrinde!elroy.jpl.nasa.gov!usc!wupost!uunet!psgrain!ee.und.ac.za!ford.ee.up.ac.za!hippo.ru.ac.za!mirk From: mirk@hippo.ru.ac.za (Prof R Kirby) Newsgroups: bionet.molbio.rapd Subject: Re: Question: Use of Ultra-pure agarose necessary.....? Message-ID: <1993Jun9.083051.18641@hippo.ru.ac.za> Date: 9 Jun 93 08:30:51 GMT References: Organization: Rhodes University, Grahamstown, South Africa Lines: 18 In msnyder@ace.acadiau.ca (MARLENE SNYDER) writes: >I am curious: We are about to start attempting some RAPD techniques here. >However, all we are intersted in is the banding patterns on the agarose (and >NOT interested in purification of bands after separation). Thus, is it >necessary to use Ultra pure (and extra expensive) Agarose? Or can we get >away with using standard lab grade agarose? Try using acrylamide and silver staining, it provides a greater amount of information per PCR and is just as good as agarose. Professor Ralph Kirby Rhodes University Grahamstown -- Prof Ralph Kirby - Department of Microbiology - Rhodes University Internet: mirk@hippo.ru.ac.za Telephone: (0461) 22023 xt 441 From owner-rapd@net.bio.net Tue Jun 08 23:00:00 1993 Path: biosci!daresbury!doc.ic.ac.uk!agate!usenet.ins.cwru.edu!gatech!rutgers!utcsri!newsflash.concordia.ca!mizar.cc.umanitoba.ca!etaylor From: etaylor@ccu.umanitoba.ca (Euan R. Taylor) Newsgroups: bionet.molbio.rapd Subject: fusion proteins- query repost Message-ID: Date: 9 Jun 93 13:42:40 GMT Sender: news@ccu.umanitoba.ca Organization: University of Manitoba, Winnipeg, Canada Lines: 15 Nntp-Posting-Host: ccu.umanitoba.ca All advice welcome. cheers Euan Usual disclaimers. These are my own opinions and probably nobody else's. etaylor@ccu.umanitoba.ca "All great truths begin life as blasphemies" George Bernard Shaw -- Usual disclaimers. These are my own opinions and probably nobody else's. etaylor@ccu.umanitoba.ca "All great truths begin life as blasphemies" George Bernard Shaw From owner-rapd@net.bio.net Wed Jun 09 23:00:00 1993 Path: biosci!ento.csiro.au!thomasb From: thomasb@ento.csiro.au Newsgroups: bionet.molbio.rapd Subject: southerns Message-ID: <9306100245.AA01626@spider.ento.csiro.au> Date: 10 Jun 93 17:45:29 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 16 Has anyone ever tried the usual Southern transfer protocols with acrylamide gels (ca. 7%), or is electroblotting the go? If its electroblotting, can I get a protocol? Thanks. ---------------------------------------------------------------------------- Thomas M. Boyce thomasb@ento.csiro.au Molecular Biology and Genetics Ph: 61-6-246-4159 Division of Entomology, CSIRO FAX: 61-6-246-4173 GPO Box 1700 Canberra, ACT 2601 AUSTRALIA From owner-rapd@net.bio.net Thu Jun 10 23:00:00 1993 Path: biosci!MBCRR.HARVARD.EDU!swiss From: swiss@MBCRR.HARVARD.EDU (Karen Swisshelm) Newsgroups: bionet.molbio.rapd Subject: RE: Question: Use of Ultra-pure agarose necessary.....? Message-ID: <9306111724.AA05050@mbcrr.harvard.edu> Date: 11 Jun 93 17:24:09 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 1 From owner-rapd@net.bio.net Thu Jun 10 23:00:00 1993 Path: biosci!YVAX.BYU.EDU!anderswr From: anderswr@YVAX.BYU.EDU (W. Ralph Andersen) Newsgroups: bionet.molbio.rapd Subject: RE: Question: Use of Ultra-pure agarose necessary.....? Message-ID: <01GZ8X10JR8I8X4UXG@yvax.byu.edu> Date: 11 Jun 93 19:38:42 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 8 I am interested in the acrylamide and silver staining. Would you please suggest a source for protocol? Thanks. Ralph Andersen 297 WIDB BYU Provo, Ut 84602 From owner-rapd@net.bio.net Thu Jun 10 23:00:00 1993 Path: biosci!daresbury!daresbury!not-for-mail From: mbfw@s-crim1.dl.ac.uk (Frank Wright) Newsgroups: bionet.molbio.rapd Subject: Analysing RAPD data and designing expts Message-ID: <1vab7mINNf1a@s-crim1.dl.ac.uk> Date: 11 Jun 93 16:17:58 GMT Organization: Daresbury Lab., Warrington, U.K. Lines: 19 NNTP-Posting-Host: s-crim1.dl.ac.uk I'm looking for a paper (in press?) that provides formulae for calculating various population genetics statistics from RAPD data and that offers advice on designing expts. A few months ago the author posted a brief summary of the paper's findings on this newsgroup (or the old RAPD-L). I've tried to locate the article by using WAIS on biosci.src but with no luck. Can anyone direct me to the original article, or (even better) provide a reference to the published paper? Thanks Frank Wright SASS, University of Edinburgh, Edinburgh, Scotland frank@sass.sari.ac.uk From owner-rapd@net.bio.net Thu Jun 10 23:00:00 1993 Path: biosci!YVAX.BYU.EDU!anderswr From: anderswr@YVAX.BYU.EDU (W. Ralph Andersen) Newsgroups: bionet.molbio.rapd Subject: RE: Question: Use of Ultra-pure agarose necessary.....? Message-ID: <01GZ8XOEFBRM8X50ER@yvax.byu.edu> Date: 11 Jun 93 19:57:33 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 9 Kirby: We use standard lab agarose low EEO (Fisher for instance) for RAPDs----but highly pure stuff for band extraction. (I don't rightly know why we use highly pure stuff for that purpose, but we do----probably because others do it and we don't dare to do otherwise----know what it mean?). Ralph Andersen From owner-rapd@net.bio.net Thu Jun 10 23:00:00 1993 Path: biosci!YVAX.BYU.EDU!anderswr From: anderswr@YVAX.BYU.EDU (W. Ralph Andersen) Newsgroups: bionet.molbio.rapd Subject: Re: Operon address Message-ID: <01GZ8XJAF5HE8X68BH@yvax.byu.edu> Date: 11 Jun 93 19:53:25 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 7 MVS; Regarding an address for "Operon". I use their free phone number: 800 688 2248. Ralph Andersen From owner-rapd@net.bio.net Sun Jun 13 23:00:00 1993 Path: biosci!esvax.dnet.dupont.com!rafalski From: rafalski@esvax.dnet.dupont.com Newsgroups: bionet.molbio.rapd Subject: Silver staining references Message-ID: <9306141318.AA28043@esds01.es.dupont.com> Date: 14 Jun 93 13:18:34 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 14 Re: Question about silver staining of RAPD gels: THere are a number of papers from Peter Gresshoff's lab (U. of Tenn). on their DAF technique involving random primers and silver staining of acrylamide gels: for example see Appl.Microbiol.Biotechnol. 38(1992) 70-76 MGG 235 (1992) 157-165. The original paper is Bio/Technology 9(1991) 553-557. I am told a kit employing their silver staining tehcnology is available from Promega. John Carlson is using silver staining with RAPDs (Gressoff et al. use a somewhat different condsitions). Also, I am told that the Phastgel system (Pharmacia) can be used. Antoni Rafalski rafalski@esvax.dnet.dupont.com From owner-rapd@net.bio.net Sun Jun 13 23:00:00 1993 Path: biosci!DAX.CC.UAKRON.EDU!r1sss From: r1sss@DAX.CC.UAKRON.EDU (Scott S Schaus) Newsgroups: bionet.molbio.rapd Subject: (none) Message-ID: <9306141741.AA10872@dax.cc.uakron.edu> Date: 14 Jun 93 17:41:02 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 10 We are sending mail to see if this address exists. If this is the proper site, send us info on how to log into the RAPD discussion forum. ****************************************************************** ** Scott S. Schaus The University of Akron ** ** r1sss@UAkron.edu Department of Biology ** ** (216) 972-7155 Akron, OH 44325-3908 ** ****************************************************************** From owner-rapd@net.bio.net Sun Jun 13 23:00:00 1993 Path: biosci!qbfelp.edu.ar!lagares From: lagares@qbfelp.edu.ar Newsgroups: bionet.molbio.rapd Subject: Transfer of polyacrilamide gels Message-ID: <067fk725@qbfelp.edu.ar> Date: 11 Jun 93 16:44:42 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 36 We never used electrotransference for polyacrilamide gels. This protocol was sent by Alena Leroux < Unit 129, INSERM, 24 rue du Fauburg Saint Jacques, 75014 Paris, Ph:43-20-12-40 int:372, FAX: 46-34-68-70> and work well for tranference of nucleic acids from 100 to 500 bp (1000 bp, may be). Standard conditions: Gel: 10 cm X 15 cm X 0.75 mm; 6-8% acrylamide; Run in TBE buffer. 1. 0.2M NaOH/0.6M NaCl for 30 minutes. Shake slowly. 2. 7% formaldehyde for 15 minutes. Twice. 3. Transfer in 10X SSC on membrane (Hybond or nitrocelulose, presoaked in 10X SSC) 4. UV 2 min. (Hybond) or baking at 80 deg. C for 2 hrs (nitrocelulose) Antonio Lagares lagares@qbfelp.edu.ar Quimica Biologica - IBBM Facultad de Ciencias Exactas Universidad Nacional de La Plata 47 y 115, 1900-LA PLATA ARGENTINA Phone: +54-21-250497 FAX: +54-21-259223 TELEX: 31151 BULAP AR; 31216 CESLA AR E-mail: lagares@qbfelp.edu.ar From owner-rapd@net.bio.net Sun Jun 13 23:00:00 1993 Path: biosci!daresbury!mrccrc!datherto From: datherto@crc.ac.uk (Mr. D.J. Atherton) Newsgroups: bionet.molbio.rapd Subject: Re: Question: Use of Ultra-pure agarose necessary.....? Message-ID: <1993Jun14.154330.17861@crc.ac.uk> Date: 14 Jun 93 15:43:30 GMT References: <01GZ8X10JR8I8X4UXG@yvax.byu.edu> Sender: news@crc.ac.uk Distribution: bionet Organization: MRC Human Genome Resource Centre Lines: 30 Nntp-Posting-Host: tin Hi Netter, IMHO, and probably a lot of others, the molbiol bible should do it:- Sambrook, Fritsch, and Maniatis Bk 1 Chap 6 for PAGE ----Same guys again--- Bk 3 Chap ? for Silver staining Silver staining method listed for protein detection, but works with DNA, or try:- Wash gel in water for 20min with gentle shaking Wash gel in 0.3% Ammonia in water for 15min Make up 150ml 0.15% Silver nitrate (AgNO3) - A clear soln then add 0.5ml freshly prepared 1M NaOH and stir vigourously - soln looks like white coffee With stirring, dropwise add conc ammonia until soln becomes clear (approx. 2ml) Wash gel in above AgNO3/NaOH soln for 15min Rinse thoroughly with water Develop stain with 2% Na2CO3/0.2% Formamide - keep an eye on it, it tends to start slow then *bang* turns dark Stop developing by copious rinsing with water. Photograph immediately - just in case Regards David Atherton datherton@uk.ac.ox.path.vax Univ. Oxford Oxford Oxon UK ! . From owner-rapd@net.bio.net Mon Jun 14 23:00:00 1993 Path: biosci!micro.uct.ac.za!ED From: ED@micro.uct.ac.za ("Ed Rybicki") Newsgroups: bionet.molbio.rapd Subject: Re: Question: Use of Ultra-pure...silver staining... Message-ID: Date: 15 Jun 93 07:51:44 GMT Sender: daemon@net.bio.net Reply-To: ed@micro.uct.ac.za Distribution: bionet Lines: 71 > Hi Netter, > > IMHO, and probably a lot of others, the molbiol bible should do it:- > Sambrook, Fritsch, and Maniatis Bk 1 Chap 6 for PAGE > ----Same guys again--- Bk 3 Chap ? for Silver staining > Silver staining method listed for protein detection, but works with DNA, > or try:- And another, from personal comm: RNA / DNA SILVER STAIN PROTOCOL FOR ACRYLAMIDE GELS FROM: Moshe Balas, Volcani Centre, Rehovot, Israel BY: Ed Rybicki, Dept Microbiology, University of Cape Town, Aug 1992 WORKS VERY WELL FOR VIROIDS, dsRNAs, AND REPUTEDLY FOR DNA ALSO SHORT PROCEDURE: 1. Soak in 30% EtOH / 10% acetic acid 10 min on bench, can leave up to O/N. 2. Soak 10% EtOH 2x10 min, wash in ddH2O 3x5 min. 3. Soak in 6 mM AgNO3 for 30 min (50 ml/gel), then quick rinse ddH2O. 4. Rinse rapidly in fresh 2.5% Na2CO3 / 0.02% HCHO, then fresh soln. for 2 min or longer: develop in same solution till bands appear dense enough. 5. Stop with 1% acetic and keep in same solution. NB: CAN DESTAIN GELS WITH 2% AMMONIUM PEROXYDISULPHATE NNB: CAN SHRINK GELS FOR STORAGE BY SOAKING IN ABSOLUTE EtOH, THEN ACETONE, THEN AIR-DRYING LONG PROCEDURE: 1. Soak polyacrylamide AFTER ELECTROPHORESIS in 50% EtOH / 10% acetic acid 1-3 hr or or overnight. 2. Soak in 10% EtOH / 1% acetic acid 1 hr, rinse 3x in ddH2O. 3. Soak in 12 mM AgNO3 1 hr, rinse 3x5 min in ddH2O. 4. Rinse rapidly in fresh 0.75 M KOH / 0.28% HCHO, leave to develop in more of same solution till bands appear at sufficient contrast / density. 5. Add XS H2O to stop or stop with 0.07 M Na2CO3 or 5% acetic acid. Store in 1% acetic. *********** ____________________________________________________________________ | Ed Rybicki, PhD | "Lord, won't you buy me | | (ed@micro.uct.ac.za) | | | Dept Microbiology | A Mer-ce-des Benz..." | | University of Cape Town | | | Private Bag, Rondebosch | | | 7700, South Africa | - Janis Joplin | | fax: 27-21-650 4023 | | -------------------------------------------------------------------- From owner-rapd@net.bio.net Tue Jun 15 23:00:00 1993 Path: biosci!uwm.edu!math.ohio-state.edu!howland.reston.ans.net!usenet.ins.cwru.edu!news.uakron.edu!dax.cc.uakron.edu!r1sss From: r1sss@dax.cc.uakron.edu (Scott S Schaus ) Newsgroups: bionet.molbio.rapd Subject: New subscribers to RAPD newsgroup Message-ID: <1993Jun16.174405.21669@news.uakron.edu> Date: 16 Jun 93 17:44:05 GMT Sender: news@news.uakron.edu Distribution: bionet Organization: The University of Akron, Akron, Ohio Lines: 15 Greetings. We would like to know where the archived file(s) are that contain all previous postings to this newgroup so that we may "catch-up" on the current trends. This info may be forwarded by E-mail so as not to tie up the discussion group. Thanks :-) Scott Schaus/Monte Turner -- ------------------------------------------------------- Scott S. Schaus The University of Akron r1sss@dax.cc.uakron.edu Department of Biology (216) 972-7155 Akron, OH 44325-3908 From owner-rapd@net.bio.net Tue Jun 15 23:00:00 1993 Path: biosci!agate!usenet.ins.cwru.edu!neoucom.edu!news.uakron.edu!dax.cc.uakron.edu!r1sss From: r1sss@dax.cc.uakron.edu (Scott S Schaus ) Newsgroups: bionet.molbio.rapd Subject: New subscriber to RAPD newsgroup Message-ID: <1993Jun16.174152.21544@news.uakron.edu> Date: 16 Jun 93 17:41:52 GMT Sender: news@news.uakron.edu Distribution: bionet Organization: The University of Akron, Akron, Ohio Lines: 10 Thanks :-) Scott Schaus/Monte Turner -- ------------------------------------------------------- Scott S. Schaus The University of Akron r1sss@dax.cc.uakron.edu Department of Biology (216) 972-7155 Akron, OH 44325-3908 From owner-rapd@net.bio.net Wed Jun 16 23:00:00 1993 Path: biosci!sfu.ca!corley From: corley@sfu.ca ("Graham Corley-Smith") Newsgroups: bionet.molbio.rapd Subject: RE: RAPD for population studies Message-ID: <59027.corley@sfu.ca> Date: 18 Jun 93 00:23:41 GMT Sender: daemon@net.bio.net Reply-To: corley@sfu.ca Distribution: bionet Lines: 33 In reply (see below) to your note: >In Message Thu, 17 Jun 93 11:04:49 EDT, C017000 writes: >Hi. I have been searching in the litterature and cannot find >anything regarding the use of RAPD to study vertebrate >populations. However, I found lots of references concerning >the use of RAPD in plant studies. Is anyone aware of >any work being done on trying to discriminate vertebrate >populations with RAPDs? >Thanks. >Marie-Claire Baby >Biology dept. >U. Laval >e-mail: c017@music.ulaval.ca Yes there are people who use RAPD analysis for animals. Publications on insects (including bees, grasshoppers and mosquitos) are available in the literature. As for vertebrates, we have recently started using RAPD's on zebrafish (Brachydanio rerio) at the Institute of Molecular Biology and Biochemistry, Biological Sciences Department, Simon Fraser University. However, Dr. John Postlewaite at the University of Oregon in Eugene Oregon, has more experience than myself with RAPD analysis of zebrafish populations and would be a good person to contact if you have specific questions. _____ Graham E. Corley-Smith Institute of Molecular Biology and Biochemistry Simon Fraser University Burnaby, B.C., V5A 1S6 Canada Phone: (604) 291-3021 Fax: (604) 291-3496 From owner-rapd@net.bio.net Wed Jun 16 23:00:00 1993 Path: biosci!MUSIC.ULAVAL.CA!C017 From: C017@MUSIC.ULAVAL.CA (C017000) Newsgroups: bionet.molbio.rapd Subject: RAPD for population studies Message-ID: <17JUN93.11967208.0033.MUSIC@MUSIC.ULAVAL.CA> Date: 17 Jun 93 15:04:49 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 11 Hi. I have been searching in the litterature and cannot find anything regarding the use of RAPD to study vertebrate populations. However, I found lots of references concerning the use of RAPD in plant studies. Is anyone aware of any work being done on trying to discriminate vertebrate populations with RAPDs? Thanks. Marie-Claire Baby Biology dept. U. Laval e-mail: c017@music.ulaval.ca From owner-rapd@net.bio.net Sun Jun 20 23:00:00 1993 Path: biosci!uwm.edu!math.ohio-state.edu!howland.reston.ans.net!usenet.ins.cwru.edu!news.uakron.edu!dax.cc.uakron.edu!r1sss From: r1sss@dax.cc.uakron.edu (Scott S Schaus ) Newsgroups: bionet.molbio.rapd Subject: Re: RAPD-L availability Message-ID: <1993Jun21.195235.12682@news.uakron.edu> Date: 21 Jun 93 19:52:35 GMT References: <204kp3INN7el@news.u.washington.edu> Sender: news@news.uakron.edu Distribution: usa Organization: The University of Akron, Akron, Ohio Lines: 21 In article <204kp3INN7el@news.u.washington.edu> toby@stein.u.washington.edu (Toby Bradshaw) writes: >A colleague of mine, lacking a newsfeed, is interested in knowing >if RAPD-L is still around. > Indeed it is! We just subscribed last week and all works fine. If you lack newsgroup facilities, you can send/receive E-Mail from biosci@net.bio.net. You can ftp into net.bio.net and obtain the BIOSCI_info.sheet for complete instructions. Good Luck, ------------------------------------------------------- Scott S. Schaus The University of Akron r1sss@dax.cc.uakron.edu Department of Biology (216) 972-7155 Akron, OH 44325-3908 ------------------------------------------------------- -- ------------------------------------------------------- Scott S. Schaus The University of Akron r1sss@dax.cc.uakron.edu Department of Biology (216) 972-7155 Akron, OH 44325-3908 From owner-rapd@net.bio.net Sun Jun 20 23:00:00 1993 Path: biosci!agate!howland.reston.ans.net!wupost!kuhub.cc.ukans.edu!hubble.asymetrix.com!netnews.nwnet.net!news.u.washington.edu!stein.u.washington.edu!toby From: toby@stein.u.washington.edu (Toby Bradshaw) Newsgroups: bionet.molbio.rapd Subject: Re: RAPD-L availability Message-ID: <205a2kINNh10@news.u.washington.edu> Date: 21 Jun 93 21:43:48 GMT References: <204kp3INN7el@news.u.washington.edu> <1993Jun21.195235.12682@news.uakron.edu> Distribution: usa Organization: University of Washington, Seattle Lines: 14 NNTP-Posting-Host: stein.u.washington.edu In article <1993Jun21.195235.12682@news.uakron.edu> r1sss@dax.cc.uakron.edu (Scott S Schaus ) writes: >In article <204kp3INN7el@news.u.washington.edu> toby@stein.u.washington.edu (Toby Bradshaw) writes: >>A colleague of mine, lacking a newsfeed, is interested in knowing >>if RAPD-L is still around. >> >Indeed it is! We just subscribed last week and all works fine. If you >lack newsgroup facilities, you can send/receive E-Mail from >biosci@net.bio.net. You can ftp into net.bio.net and obtain the >BIOSCI_info.sheet for complete instructions. Thanks to you and Jim Farmer for quick responses. -Toby Bradshaw toby@u.washington.edu From owner-rapd@net.bio.net Sun Jun 20 23:00:00 1993 Path: biosci!agate!howland.reston.ans.net!darwin.sura.net!news-feed-1.peachnet.edu!umn.edu!gaia.ucs.orst.edu!osshe.edu!news.uoregon.edu!netnews.nwnet.net!news.u.washington.edu!stein.u.washington.edu!toby From: toby@stein.u.washington.edu (Toby Bradshaw) Newsgroups: bionet.molbio.rapd Subject: RAPD-L availability Message-ID: <204kp3INN7el@news.u.washington.edu> Date: 21 Jun 93 15:40:19 GMT Distribution: usa Organization: University of Washington, Seattle Lines: 8 NNTP-Posting-Host: stein.u.washington.edu A colleague of mine, lacking a newsfeed, is interested in knowing if RAPD-L is still around. Toby Bradshaw | Department of Biochemistry | Will make genetic linkage maps and College of Forest Resources | for food. University of Washington, Seattle | toby@u.washington.edu | From owner-rapd@net.bio.net Mon Jun 21 23:00:00 1993 Path: biosci!UMBSKY.CC.UMB.EDU!shiaris From: shiaris@UMBSKY.CC.UMB.EDU (MICHAEL SHIARIS) Newsgroups: bionet.molbio.rapd Subject: Bacterial DNA extractions for RAPDs Message-ID: <199306221528.AA00991@ns.umb.edu> Date: 22 Jun 93 15:28:06 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 17 Does anyone have a genomic DNA extraction procedure for bacteria that will produce DNA pure enough for RAPD PCR? We are looking at environmental isolates that produce copious polysaccharides that are difficult to remove. Currently we are using a lysozyme-SDS procedure followed by 3 to 4 phenol/chloroform extractions. We have tried the CTAB method (from Current Protocols in Molecular Biology), but this still requires a follow up with phenol/chloroform. Most of the isolates that we are working with are pseudomonads from marine sediments. Thanks. Michael Shiaris Dept. Biology shiaris@umbsky.cc.umb.edu Univ. Massachusetts/Boston 100 Morrissey Blvd. Boston, MA 02125 From owner-rapd@net.bio.net Mon Jun 21 23:00:00 1993 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Doug Rhoads") Newsgroups: bionet.molbio.rapd Subject: Re: use of RAPDs as population markers Message-ID: <120C84D1BFC@uamercury.uark.edu> Date: 22 Jun 93 14:46:21 GMT Sender: daemon@net.bio.net Distribution: bionet Organization: University of Arkansas Lines: 42 In a discussion of vertebrate RAPDs Marie-Claire Baby states- >Thanks for your answer to my message. Yes, I have a specific >question. I have not yet chosen the method I am going to use >to realize my project right now, but I was wondering if >it is possible to find distinct populations markers using >RAPDs. By distinct markers, I mean something that would be >unique to that population, probably using a combination of >results obtained with various primers, instead of relying >on frequencies like with allozymes and mtDNA. >I will be working on fish populations and I don't know >exactly what to expect. So far, we have tried the method >on one population of atlantic tomcod and have obtained >nice amplifications, but we still have to assay other populations >to determine inter-population variability. Do you think that >it is realistic to hope to find such distinct population >markers? >Marie-Claire Baby >c017@music.ulaval.ca I really don't think you will find 'populations specific markers' (psm?) as this would require that all members of a population derive from an immutable locus from a single monophyletic source (founder). The likelihood that the population has been separated long enough and yet have not diverged from a single ancestor (which is unlikely) is remote. You are asking for the population to have converged on a particular sequence. I think in terms of frequencies of loci in a population you might be able to bulk (ala Tanksley) DNAs from the population and then have particular bands that NEVER show up in a population or a band that is ONLY present in one population (note:not present in ALL members of the population. These are negative experiments but I think they hold more promise than looking for a marker that is present in all members of a population that is absent in all other populations. Any one else have an opinion on this?? better yet facts!! Doug Rhoads || Dept. of Biological Sciences drhoads@mercury.uark.edu || 601 Science Engineering drhoads@uafsysb.uark.edu || University of Arkansas 501-575-3251 || Fayetteville, AR 72701 From owner-rapd@net.bio.net Mon Jun 21 23:00:00 1993 Path: biosci!parc!decwrl!decwrl!pdq.coe.montana.edu!news.uoregon.edu!netnews.nwnet.net!news.u.washington.edu!stein.u.washington.edu!toby From: toby@stein.u.washington.edu (Toby Bradshaw) Newsgroups: bionet.molbio.rapd Subject: Re: use of RAPDs as population markers Message-ID: <2076l6INNsgj@news.u.washington.edu> Date: 22 Jun 93 14:57:42 GMT References: <120C84D1BFC@uamercury.uark.edu> Distribution: bionet Organization: University of Washington, Seattle Lines: 55 NNTP-Posting-Host: stein.u.washington.edu In article <120C84D1BFC@uamercury.uark.edu> DRHOADS@MERCURY.UARK.EDU ("Doug Rhoads") writes: >In a discussion of vertebrate RAPDs Marie-Claire Baby states- >>I was wondering if >>it is possible to find distinct populations markers using >>RAPDs. By distinct markers, I mean something that would be >>unique to that population, probably using a combination of >>results obtained with various primers, instead of relying >>on frequencies like with allozymes and mtDNA. >>I will be working on fish populations and I don't know >>exactly what to expect. So far, we have tried the method >>on one population of atlantic tomcod and have obtained >>nice amplifications, but we still have to assay other populations >>to determine inter-population variability. Do you think that >>it is realistic to hope to find such distinct population > I really don't think you will find 'populations specific markers' >(psm?) as this would require that all members of a population derive from >an immutable locus from a single monophyletic source (founder). Loss of markers at the "standard" mutation rate won't have a measurable effect on most population genetics work, so I wouldn't worry about "immutable". >The >likelihood that the population has been separated long enough and yet have >not diverged from a single ancestor (which is unlikely) is remote. You >are asking for the population to have converged on a particular sequence. >I think in terms of frequencies of loci in a population you might be able >to bulk (ala Tanksley) DNAs from the population and then have particular >bands that NEVER show up in a population or a band that is ONLY >present in one population (note:not present in ALL members of the >population. These are negative experiments but I think they hold more >promise than looking for a marker that is present in all members of a >population that is absent in all other populations. > > Any one else have an opinion on this?? better yet facts!! In the extreme case of population differentiation, speciation, we have found that about 60% of the RAPDs we mapped in an F2 of interspecific hybrids of Populus are fixed (judged by sampling 10 indiviudals from each species, so hardly a rigorous test for true "fixation") for alternative alleles in the two parental species, hence are useful for mapping in all such interspecific F2s. I would guess than in populations where drift is a significant force in evolution, it will possible to find markers unique to a population (only because it is possible to sample many, many loci but impossible to sample all individuals of all populations for most species, so that low frequency is practically equivalent to fixation). Toby Bradshaw | Department of Biochemistry | Will make genetic linkage maps and College of Forest Resources | for food. University of Washington, Seattle | toby@u.washington.edu | From owner-rapd@net.bio.net Mon Jun 21 23:00:00 1993 Path: biosci!MTS.UCS.UALBERTA.CA!USERFG13 From: USERFG13@MTS.UCS.UALBERTA.CA Newsgroups: bionet.molbio.rapd Subject: (none) Message-ID: <3680038@mts.ucs.ualberta.ca> Date: 21 Jun 93 23:11:38 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 1 SIGNOFF RAPD-L DENIAL CHONG From owner-rapd@net.bio.net Mon Jun 21 23:00:00 1993 Path: biosci!BU-BIO.BU.EDU!williams From: williams@BU-BIO.BU.EDU (Scott Williams) Newsgroups: bionet.molbio.rapd Subject: RE: RAPDs as population markers Message-ID: <9306221418.AA03212@bu-bio.bu.edu> Date: 22 Jun 93 14:18:54 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 21 Doug Rhoads recently argued that RAPDs will not be good as diagnostic markers for populations becasue it is unlikely that a single fragment will be fixed in a population ans presumably absent from other ]populations. I agree with this assessment, however, that does not necessariuly invalidate the use of RAPDs for population differentiation. Assume you find one marker band using a single primer that is unique to a population and in 30% of the individuals o this population. If everything is carefully controlled and tested then the presence of this band can be used to conclusively say those individuals carrying it are from that population (population A, say). Using a second primer you find that anothger fragment is unique to population A and is in 40@ of the individuals (some identical to the first group of individuals some not). This increases to pinpoint the population of origin. With a third and fourht primer you should follow the same logic to actually do a fairly good job of determining the populations of origin. Using this sequential method may allow quite good resiloving power. Scott Williams From owner-rapd@net.bio.net Tue Jun 22 23:00:00 1993 Path: biosci!YVAX.BYU.EDU!anderswr From: anderswr@YVAX.BYU.EDU (W. Ralph Andersen) Newsgroups: bionet.molbio.rapd Subject: Re: RAPD for population studies Message-ID: <01GZPXW7U94Y8X9QV1@yvax.byu.edu> Date: 24 Jun 93 00:07:37 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 3 Yes! Try Dennis Shiazawa, Paul Evans in Current Contents author search (1992-1993 should pick up a paper or two). From owner-rapd@net.bio.net Thu Jun 24 23:00:00 1993 Path: biosci!NET.BIO.NET!kristoff From: kristoff@NET.BIO.NET (David Kristofferson) Newsgroups: bionet.molbio.rapd Subject: IMPORTANT BIOSCI INFORMATION Message-ID: <9306250900.AA00896@net.bio.net> Date: 25 Jun 93 09:00:03 GMT Sender: kristoff@net.bio.net Distribution: bionet Lines: 143 Three important items follow: BIOSCI archive searching by e-mail, the BIOSCI FAQ, and the BIOSCI User Address Directory form. 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Thanks again for your cooperation! --------------- please cut here and return portion below --------------- New information or Update to old record (enter N or U): date (DD-MM-YY): first name: middle initial: family name: job title: e-mail address: e-mail network: phone number: FAX number: institution: address1: address2: address3: city: state/province: country: postal code: research interest: research interest: comment: comment: comment: comment: comment: From owner-rapd@net.bio.net Thu Jun 24 23:00:00 1993 Path: biosci!parc!decwrl!decwrl!usenet.coe.montana.edu!netnews.nwnet.net!ns1.nodak.edu!plains!mcclean From: mcclean@plains (Phillip McClean) Newsgroups: bionet.molbio.rapd Subject: AFLP Info Message-ID: Date: 25 Jun 93 18:51:15 GMT Sender: usenet@ns1.nodak.edu (Usenet login) Organization: North Dakota Higher Education Computing Network Lines: 6 Nntp-Posting-Host: plains.nodak.edu X-Newsreader: Tin 1.1 PL4 I recently became aware of AFLPs. Does anyone have any info to share regarding this technology? Phil McClean North Dakota State University mcclean@plains.nodak.edu From owner-rapd@net.bio.net Fri Jun 25 23:00:00 1993 Path: biosci!JESTER.USASK.CA!lur From: lur@JESTER.USASK.CA (Rui Lu) Newsgroups: bionet.molbio.rapd Subject: (none) Message-ID: <9306260037.AA13156@jester.usask.ca> Date: 26 Jun 93 00:37:10 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 1 signoff rapd-l RUI LU From owner-rapd@net.bio.net Fri Jun 25 23:00:00 1993 Path: biosci!MACC.WISC.EDU!JCHEN From: JCHEN@MACC.WISC.EDU (Jianchi Chen) Newsgroups: bionet.molbio.rapd Subject: Stoffel fragment? Message-ID: <23062601122202@vms2.macc.wisc.edu> Date: 26 Jun 93 06:12:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 11 Hi, netters, Can anybody explain me what is "Stoffel fragment"? I saw it in some previous postings. J.Chen Department of Food Microbioloby and Toxicology University of Wisconsin JCHEN@MACC.WISC.EDU From owner-rapd@net.bio.net Sun Jun 27 23:00:00 1993 Path: biosci!esvax.dnet.dupont.com!rafalski From: rafalski@esvax.dnet.dupont.com Newsgroups: bionet.molbio.rapd Subject: AFLPs Message-ID: <9306281205.AA13702@esds01.es.dupont.com> Date: 28 Jun 93 12:05:02 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 5 Regarding a question from Phil McClean : To find out more about AFLPs read European patent application 92402629.7 (Eoropean Patent Office Publication Number 0 534 858 A1) Antoni From owner-rapd@net.bio.net Sun Jun 27 23:00:00 1993 Path: biosci!micro.uct.ac.za!ED From: ED@micro.uct.ac.za ("Ed Rybicki") Newsgroups: bionet.molbio.rapd Subject: Re: AFLP Info Message-ID: Date: 28 Jun 93 07:54:41 GMT Sender: daemon@net.bio.net Reply-To: ed@micro.uct.ac.za Distribution: bionet Lines: 51 > To: rapd@net.bio.net > From: mcclean@plains (Phillip McClean) > Subject: AFLP Info > Date: 25 Jun 93 18:51:15 GMT > I recently became aware of AFLPs. Does anyone have any info to share > regarding this technology? Yes - we had a talk from one of the originators of the technique, which is proprietary to KEYGENE (NV?) of The Netherlands. Apparently all is to be published soon, and vast amounts of data will be unleashed on the unsuspecting RAPD community, and it will look like the best thing since PCR (sorry, thermal cycling). Basically, one chops up genomic DNA using a six cutter, end-labels with biotin, and then chops up all with a four-cutter. 6-cutter gives (thumb- suck) 10exp6 frags from (eg) tobacco genome; 4-cutter gives (ts) 10exp8...but if you hook out only biotin-labelled frags, get only what was 6-cut and has at least one labelled end (eg 10exp6 frags, now of smaller average size). Then ligate on linkers, and PCR (sorry, tc) with primers which are longer by one base (or two, or three) than the linker: thus only one in 4 (or 16, or 64) of primers will elongate; multiply probabilities by other primer possibilities (1 in 16, or 256, or 4096) and you see how one can fine-tune number of fragments amplified. And fragments will amplify very nicely, as discrete and sharp bands, because one is using long primers and conditions ensuring near-or perfect match. Run out on sequencing-type acrylamide gels, and get 10exp2 or 3 bands, razor-sharp, reproducible (NB: IN THEIR HANDS!!!). Touted as THE answer to screening for genetic diversity/near isogenic line differences/markers - IF DONE BY THEIR PROTOCOLS - AND ALL IS PATENTED!!! VERY impressive in presentation, and Keygene are closely tied up with a number of Dutch companies, using the technology to screen their products. They will license labs to do it, given payment of fee (large, I understand), and use of their tech/methods/software. Sorry, can't find card - but a directory of Dutch Biotech companies will locate them very quickly. ____________________________________________________________________ | Ed Rybicki, PhD | "Lord, won't you buy me | | (ed@micro.uct.ac.za) | | | Dept Microbiology | A Mer-ce-des Benz..." | | University of Cape Town | | | Private Bag, Rondebosch | | | 7700, South Africa | - Janis Joplin | | fax: 27-21-650 4023 | | -------------------------------------------------------------------- From owner-rapd@net.bio.net Sun Jun 27 23:00:00 1993 Path: biosci!uwm.edu!ux1.cso.uiuc.edu!howland.reston.ans.net!wupost!uunet!mcsun!sun4nl!sun2.iend.wau.nl!PVECK@rcl.wau.nl From: pveck@rcl.wau.nl Newsgroups: bionet.molbio.rapd Subject: Re: AFLP Info Message-ID: Date: 28 Jun 93 10:06:26 GMT References: Sender: news@sun2.iend.wau.nl (Newsmanager WAU) Reply-To: pveck@rcl.wau.nl Organization: Wageningen Agricultural University Lines: 32 In article , mcclean@plains (Phillip McClean) writes: >I recently became aware of AFLPs. Does anyone have any info to share >regarding this technology? > >Phil McClean >North Dakota State University >mcclean@plains.nodak.edu Yes, I do know about AFLP, although I do not know whether we mean the same technique. AFLP, Amplified Fragm.Length Polymorphism is a technique developed by a company called KeyGene, SciencePark, Wageningen, The Netherlands It combines the best of a PCR based technique with the reproduciblity of RFLPs. The technique is patented, and will be commercialized in a kit. Outline: 4 Restriction digestion Sticky end ligation with 'adaptor' annealing with primer which is basepairing with the adaptor and the restriction site and two or three extra nucleotides. This is a selective step, because only a few of all restriction fragments will be compatible with the primer. PCR separation. I'm leaving out details which are not necessairy to understand the principle. The results are amazing. Reproducible patterns which monitor about 100 loci simultaneously. Herman van Eck From owner-rapd@net.bio.net Sun Jun 27 23:00:00 1993 Path: biosci!ACD.TUSK.EDU!PRAKASH From: PRAKASH@ACD.TUSK.EDU Newsgroups: bionet.molbio.rapd Subject: Answer to what is Stoffel Fragment? Message-ID: <01GZWT2J02EA0000MP@Acd.Tusk.Edu> Date: 28 Jun 93 17:05:49 GMT Sender: daemon@net.bio.net Distribution: bionet Organization: Tuskegee University Lines: 28 J. Chen of Univ. of Wisconsin asked what is Stoffel Fragment? Stoffel fragment is the truncated version of the Amplitaq DNa polymerase. About 289 aminoacids in the N-terminal region are missing in Stoffel. Stoffel fragment has higher thermostability, no 3' to 5' or 5' to 3' exonuclease activity but lower processivity, so you have to use twice as much as Amplitaq. We find Stoffel fragment to be better than Amplitaq in our RAPD analysis of sweetpotato genotypes as they produce more complex and thus informative polymorphisms. US Biochemicals also produce their version of Stoffel called as DeltaTaq. We find it produces very similar results as Stoffel. To my knowledge, no one (including those at Cetus) knows why Stoffel works better. C. S. Prakash Tuskegee University (205) 727 8023 Prakash@Tusk. Edu From owner-rapd@net.bio.net Sun Jun 27 23:00:00 1993 Path: biosci!daresbury!daresbury!news From: UDBR150@OAK.CC.KCL.AC.UK Newsgroups: bionet.molbio.rapd Subject: sequencing Message-ID: <1993Jun28.144514.3082@gserv1.dl.ac.uk> Date: 28 Jun 93 14:45:00 GMT Sender: list-admin@daresbury.ac.uk Distribution: bionet Lines: 5 Original-To: RAPD@UK.AC.DARESBURY has anyone out ther tried the new promega silver staining kit for DNA sequencing. Any info on price, efficiency, value, and basicaly is it worth the money? All comments wanted Dave Roberts, Kings college London From owner-rapd@net.bio.net Mon Jun 28 23:00:00 1993 Path: biosci!JESTER.USASK.CA!lur From: lur@JESTER.USASK.CA (Rui Lu) Newsgroups: bionet.molbio.rapd Subject: (none) Message-ID: <9306291913.AA12181@jester.usask.ca> Date: 29 Jun 93 19:13:23 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 1 SIGNOFF Rui Lu From owner-rapd@net.bio.net Mon Jun 28 23:00:00 1993 Path: biosci!JESTER.USASK.CA!lur From: lur@JESTER.USASK.CA (Rui Lu) Newsgroups: bionet.molbio.rapd Subject: (none) Message-ID: <9306291913.AA12162@jester.usask.ca> Date: 29 Jun 93 19:13:04 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 1 SIGNOFF RAPD-L Rui Lu From owner-rapd@net.bio.net Mon Jun 28 23:00:00 1993 Path: biosci!YVAX.BYU.EDU!anderswr From: anderswr@YVAX.BYU.EDU (W. Ralph Andersen) Newsgroups: bionet.molbio.rapd Subject: (none) Message-ID: <01GZYCXI1C9E8WXKSU@yvax.byu.edu> Date: 30 Jun 93 00:44:15 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 15 Anybody see this problem? We have seen it with amplitaq and occaisionally with Stoffel. We are screening two sugarcane parents for RAPD polymorphisms (a polymorphism in one parent and not the other). Then we screen a sample of their progeny to prove heterozygosity for the marker. Then we screen the mapping porgeny for segregation patterns for the single-parent-heterozygous marker. Now and then we will find that a given primer will show faithful and numerous bands in the parents, but will show only a single major band when applied to all the progeny. This anomally will appear throughout the progeny----one major band is amplified and there are a few dim background bands. We are reasonably sure that we are not making mistakes in cocktail makeup. (We use the Biomek). What's wrong with us? Ralph From owner-rapd@net.bio.net Tue Jun 29 23:00:00 1993 Path: biosci!PSUVM.PSU.EDU!DBF1 From: DBF1@PSUVM.PSU.EDU ("Douglas Furtek") Newsgroups: bionet.molbio.rapd Subject: Unexplained absence of bands Message-ID: <9306300016.AA07581@net.bio.net> Date: 30 Jun 93 00:18:00 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 7 Ralph, nothing is wrong. Check out "M. Heun and T. Heltentjaris, Inheritance of RAPDs in F1 hybrids of corn, TAG (1993) 85:961-968." To quote the authors,"Nevertheless, since many of our unexplained results could be attributed to the 'competition' amongst fragments where one very intense product seemed to outcompete and overwhelm other expected fragments, even those of very high yields in inbred situations, one should use caution with certain applications." (We have not seen this with cacao - yet.) Good luck. From owner-rapd@net.bio.net Wed Jun 30 23:00:00 1993 Path: biosci!agate!howland.reston.ans.net!darwin.sura.net!news-feed-1.peachnet.edu!umn.edu!gaia.ucs.orst.edu!osshe.edu!news.uoregon.edu!netnews.nwnet.net!news.u.washington.edu!stein.u.washington.edu!toby From: toby@stein.u.washington.edu (Toby Bradshaw) Newsgroups: bionet.molbio.rapd Subject: Re: Addresses of primer makers Message-ID: <20ve3c$9r3@news.u.washington.edu> Date: 1 Jul 93 19:31:56 GMT References: <01H00ZRP35J68WYG4X@yvax.byu.edu> Distribution: bionet Organization: University of Washington, Seattle Lines: 14 NNTP-Posting-Host: stein.u.washington.edu In article <01H00ZRP35J68WYG4X@yvax.byu.edu> anderswr@YVAX.BYU.EDU (W. Ralph Andersen) writes: >We have screened all of Operon 10-mer primers for a project. I have >misplaced my file on addresses of other manufacturers of our favorite RAPDs >primers. Can anyone help me out on this? I need some addresses of good >tried and true companies----a couple of addresses will probably do the job. You can also just use pairs of the Operon primers, giving you n^2 primers to try. It's worked well for me. Toby Bradshaw | Department of Biochemistry | Will make genetic linkage maps and College of Forest Resources | for food. University of Washington, Seattle | toby@u.washington.edu | From owner-rapd@net.bio.net Wed Jun 30 23:00:00 1993 Path: biosci!YVAX.BYU.EDU!anderswr From: anderswr@YVAX.BYU.EDU (W. Ralph Andersen) Newsgroups: bionet.molbio.rapd Subject: Addresses of primer makers Message-ID: <01H00ZRP35J68WYG4X@yvax.byu.edu> Date: 1 Jul 93 22:00:10 GMT Sender: daemon@net.bio.net Distribution: bionet Lines: 6 We have screened all of Operon 10-mer primers for a project. I have misplaced my file on addresses of other manufacturers of our favorite RAPDs primers. Can anyone help me out on this? I need some addresses of good tried and true companies----a couple of addresses will probably do the job. Thanks. Ralph