From owner-rapd@net.bio.net Fri Dec 02 22:00:00 1994 Newsgroups: bionet.molbio.rapd Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!netnews.nwnet.net!ns1.nodak.edu!hugo From: e_hugo@dsu1.dsu.nodak.edu (Eric R. Hugo) Subject: Selection of primers--opinions sought Sender: usenet@ns1.nodak.edu (Usenet login) Message-ID: Date: Sat, 3 Dec 1994 23:22:02 GMT Nntp-Posting-Host: hugo.chem.dsu.nodak.edu Organization: Dickinson State University X-Newsreader: News Xpress Version 1.0 Beta #1 Keywords: oligonucleotides, arthropods Lines: 18 Dear Bionetters, I have several students here at DSU interested in starting a project using RAPD on arachnid DNA. To start things up I thought the most economical approach would be to purchase a primer set from the individuals at UBC. Our problem is which subset the 800 primers UBC is offering should we start with? Do any of you have any suggestions with resect to this? Thanks for any feedback. Sincerely, Eric // \\ // \\ // \\ // \\ // \\ // \\ // \\ Eric Hugo, Ph.D.// |:,\\': | \\ // | :,\\': | \\ // | :,\\': | \\ e_hugo@dsu1.dsu.nodak.edu\ | | \\ // | | // \\ | | \\ // | | // \\ | | Asst. Professor, Biology \\ | :,\\': | // \\ | :,\\': | // \\ | Dickinson State University \\ // \\ // \\ // \\ // \\ Dickinson, ND 58601 |PGP 2.6 Key available from most key servers From owner-rapd@net.bio.net Sat Dec 03 22:00:00 1994 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.molbio.rapd Subject: Re: Selection of primers--opinions sought Date: 4 Dec 1994 07:24:21 -0800 Organization: University of Arkansas Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <2A835D535CA@uamercury.uark.edu> NNTP-Posting-Host: net.bio.net > To: rapd@net.bio.net > From: e_hugo@dsu1.dsu.nodak.edu (Eric R. Hugo) > Subject: Selection of primers--opinions sought > Date: Sat, 3 Dec 1994 23:22:02 GMT > Dear Bionetters, > I have several students here at DSU interested in starting a project using > RAPD on arachnid DNA. To start things up I thought the most economical approach > would be to purchase a primer set from the individuals at UBC. Our problem is w > hich > subset the 800 primers UBC is offering should we start with? Do any of you have > any suggestions with resect to this? Thanks for any feedback. > Sincerely, > Eric Any set. They all work. My personal favorite is set 8 because it is a 2- and 3-base set. All of the primers in the first 7 sets work well and we have about a 60-70% success rate for genomes >10^8 bp. Set 8 gives more like 90%. However, by playing with buffer concentrations you can even get the 4base primers to work for 90% of the primers. As to the number of bands to expect is genome size and base composition dependent. Your choice depends on what you want to do: linkage maps, genetic diversity estimation, or positional mapping. Doug Rhoads || Dept. of Biological Sciences drhoads@mercury.uark.edu || 601 Science Engineering drhoads@uafsysb.uark.edu || University of Arkansas 501-575-3251 || Fayetteville, AR 72701 From owner-rapd@net.bio.net Sun Dec 04 22:00:00 1994 Path: biosci!agate!howland.reston.ans.net!pipex!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: Andrea Klabouchova Newsgroups: bionet.molbio.rapd Subject: ?RAPD(synechococc. cyanobacteria)????? Date: 5 Dec 1994 14:41:32 -0000 Lines: 6 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3bv8qs$l4d@mserv1.dl.ac.uk> Original-To: rapd@dl.ac.uk Hello netters out there! Does anybody know the strategy of DNA isolation from cyanobacteria (especially Synechococcus) for RAPD? Is there any discussion group on cyanobacteria? Thanks Andrea e-mail: andrea@jcu.cz From owner-rapd@net.bio.net Sun Dec 04 22:00:00 1994 Path: biosci!agate!howland.reston.ans.net!gatech!swrinde!pipex!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: Andrea Klabouchova Newsgroups: bionet.molbio.rapd Subject: ?RAPD (Synechococc.Cyanobact.)????? Date: 5 Dec 1994 14:02:33 -0000 Lines: 5 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3bv6hp$j85@mserv1.dl.ac.uk> Original-To: rapd@dl.ac.uk Hello netters out there! Does anybody know the strategy of DNA isolation for RAPD? Is there any special discussion group on cyanobacteria? Andrea e-mail: andrea@jcu.cz From owner-rapd@net.bio.net Sun Dec 04 22:00:00 1994 Path: biosci!daresbury!not-for-mail From: "Jacqueline Vanderstappen" Newsgroups: bionet.molbio.rapd Subject: digital storage of RAPD patterns Date: 5 Dec 1994 13:53:32 -0000 Lines: 18 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3bv60s$iqu@mserv1.dl.ac.uk> X-Incognito-Format: VERSION=1.60e ENCRYPTED=NO X-Incognito-SN: 297 Original-To: rapd@dl.ac.uk Hello, I would like to analyse my RAPD bands by computer. Therefore I need a system which can store the picture in a format (e.g. tiff) used by the computer analysis software. I would like to know about your experiences with the available systems (scanning or directly storage from a U.V. visualized gel by means of a video card). If possible, I would like the addresses of the suppliers here in Europe. Thank you in advance, Vander Stappen Jacqueline Research Assistant KULeuven Laboratorium voor Gentechnologie W.Decroylaan 42 3001 Heverlee Belgium From owner-rapd@net.bio.net Sun Dec 04 22:00:00 1994 Path: biosci!rutgers!gatech!swrinde!howland.reston.ans.net!vixen.cso.uiuc.edu!news.uoregon.edu!gaia.ucs.orst.edu!sslab.FSL.ORST.EDU!krutovskiik From: krutovskiik@fsl.orst.edu (Konstantin Krutovskii) Newsgroups: bionet.molbio.rapd Subject: Re: Stratagen Taq Extender Date: Mon, 5 Dec 1994 12:03:11 Organization: fsl.orst.edu Lines: 43 Distribution: world Message-ID: References: <19EBB2C5905@uamercury.uark.edu> NNTP-Posting-Host: sslab.fsl.orst.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] In article <19EBB2C5905@uamercury.uark.edu> DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") writes: >From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") >Subject: Re: Stratagen Taq Extender >Date: 23 Nov 1994 05:57:44 -0800 >> To: rapd@net.bio.net >> From: krutovskiik@fsl.orst.edu (Konstantin Krutovskii) >> Subject: Stratagen Taq Extender >> Date: Tue, 22 Nov 1994 19:32:28 >> Stratagen recently introduced very effective PCR-enhancement reagent, Taq >> Extender PCR additive (see Nielson et al., 1994: Strategies 7: 26 or 7:64). >> We use it in the same amount per reaction as Taq. It really improves the >> amplification of different templates and increases the yield of many PCRs >> (especially for long products). I hope it would be interesting for RAPD folks >> who got headache with difficult templates. >> >> Kostya >But will Taq extender work under RAPD conditions?? Has anyone tried? > Some enzyme preps of Taq contain lots of contaminating fragmented >DNAs that generate smears under RAPD conditions with most primers and >no additional input template DNA. We have measured as high as >300ng/ul DNA in some commercial preps of Taq Pol. >Doug Rhoads || Dept. of Biological Sciences >drhoads@mercury.uark.edu || 601 Science Engineering >drhoads@uafsysb.uark.edu || University of Arkansas >501-575-3251 || Fayetteville, AR 72701 Yes, we use extender together with Taq under regular RAPD conditions. It has the same all problems as the using of Taq alone, but it helps to amplify larger amount of long and middle size DNA products, (very often, unfortunately, at the expense of short size fragments (!). Thus, using extender one can sometimes lose some bands (usually, short fragments according to our experience) which were well amplified under regular conditions, but can also very likely obtaine other bands (mostly long fragments) and increase amplification of many products (long and middle fragments). Anyway, the using of extender (of course, only together with Taq!) gives a larger number of well amplified bands in general, than the using Taq alone. Kostya From owner-rapd@net.bio.net Sun Dec 04 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!pipex!sunic!trane.uninett.no!due.uninett.no!alf.uib.no!usenet From: Ruth.Sandaa@im.uib.no Newsgroups: bionet.molbio.rapd Subject: Bead Beater Date: 5 Dec 1994 10:33:35 GMT Organization: University of Bergen, Norway Lines: 6 Message-ID: <3buq9v$fai@alf.uib.no> NNTP-Posting-Host: pc34.im.uib.no X-Newsreader: News X1.0-28 (Win32) Hello, out there. I'm looking for information about a product called Bead Beater, manufactured by BioSpec Products, Bartlesville, Oklahoma. Could anybody please provide me with a more excat address or may be a fax number. Ruth-Anne From owner-rapd@net.bio.net Mon Dec 05 22:00:00 1994 Newsgroups: bionet.molbio.rapd Path: biosci!bloom-beacon.mit.edu!usc!howland.reston.ans.net!Germany.EU.net!EU.net!sun4nl!rivm!news From: lwlkarel@rivm.nl (K. Wernars) Subject: Re: ?RAPD (Synechococc.Cyanobact.)????? Message-ID: Sender: news@rivm.nl Organization: Natl. Inst. of Publ. Health and Env. Protection X-Newsreader: Distribution: bionet Date: Tue, 6 Dec 1994 10:27:22 GMT Lines: 24 Andrea Klabouchova wrote: >> Hello netters out there! >> Does anybody know the strategy of DNA isolation for RAPD? >> Is there any special discussion group on cyanobacteria? >> Andrea e-mail: andrea@jcu.cz The best strategy to isolate DNA for RAPD-typing of bacteria is no DNA isolation. For many genera (e.g. Listeria, Bacillus, Pleisiomonas) it is possible to add the whole cells directly to the PCR-reaction mixture and get perfectly reproducible profiles. For other genera it is necessary (for the sake of reproducibility) to make a boiled lysate, clear it by centrifugation and add this to the reaction mixture. You can read about it in "Res. Microbiol., 1992, 143, 499-505" and "Lett. Appl. Microbiol., 1992, 14, 260-2. If you are interested to receive a lab-protocol please send me an email. ****************************** Karel Wernars, The Netherlands ****************************** From owner-rapd@net.bio.net Mon Dec 05 22:00:00 1994 Newsgroups: bionet.molbio.rapd Path: biosci!ns1.faseb.org!darwin.sura.net!nih-csl!postman From: kvernick@helix.nih.gov Subject: Re: Bead Beater Content-Type: TEXT/PLAIN; charset=US-ASCII Message-ID: <1994Dec5.234638.8443@alw.nih.gov> Sender: postman@alw.nih.gov (AMDS Postmaster) Organization: National Institutes of Health References: <3buq9v$fai@alf.uib.no> Mime-Version: 1.0 Date: Tue, 6 Dec 1994 02:49:21 GMT Lines: 22 In article <3buq9v$fai@alf.uib.no>, writes: > Path: nih-csl!lhc!biosci!bloom-beacon.mit.edu!gatech!swrinde!pipex!sunic!trane.uninett.no!due.u ninett.no!alf.uib.no!usenet > From: Ruth.Sandaa@im.uib.no > Newsgroups: bionet.molbio.rapd > Subject: Bead Beater > Date: 5 Dec 1994 10:33:35 GMT > Organization: University of Bergen, Norway > Lines: 6 > Message-ID: <3buq9v$fai@alf.uib.no> > NNTP-Posting-Host: pc34.im.uib.no > X-Newsreader: News X1.0-28 (Win32) > > Hello, out there. I'm looking for information about a product called Bead Beater, > manufactured by BioSpec Products, Bartlesville, Oklahoma. Could anybody > please provide me with a more excat address or may be a fax number. > > Ruth-Anne > The BeadBeater Homogenizer comes from Biospec Products, phone 918-336-3363 From owner-rapd@net.bio.net Mon Dec 05 22:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!munnari.oz.au!newsroom.utas.edu.au!mg1_104.plant.utas.edu.au!user From: Kath.Nesbitt@plant.utas.edu.au () Newsgroups: bionet.molbio.rapd Subject: RAPD fingerprinting Followup-To: bionet.molbio.rapd Date: Tue, 06 Dec 1994 12:28:51 +1000 Organization: University of Tasmania Lines: 7 Message-ID: NNTP-Posting-Host: mg1_104.plant.utas.edu.au Hello people, does anyone have any thoughts on using RAPDSs for fingerprinting. Are they good/bad/just as good as anything else. I'm particularly concerned with their economic feasability and their transferability from lab to lab. I'd be interested in anything anyone has to say so please reply! Thanks Kath From owner-rapd@net.bio.net Mon Dec 05 22:00:00 1994 Newsgroups: bionet.molbio.rapd Path: biosci!rutgers!gatech!swrinde!pipex!uunet!zib-berlin.de!ceres.fokus.gmd.de!nntp.gmd.de!urmel.informatik.rwth-aachen.de!newsserver.rrzn.uni-hannover.de!web.pci.chemie.uni-tuebingen.de!cbiei01 From: cbiei01@commlink.zdv.uni-tuebingen.de (Harold V. Taylor) Subject: acronyms Message-ID: Lines: 25 Sender: news@newsserver.rrzn.uni-hannover.de (News Service) Organization: Physiol. Chem. Institut, Universitaet Tuebingen, Germany X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #1] Date: Tue, 6 Dec 1994 16:39:55 GMT In article <199411291651.IAA00612@net.bio.net> fishgen@vt.edu (Bruce J. Turner) writes: >I thought I was fairly up-t0--date on acronyms, I'd appreciate information on where to find acronym-lists (e.g., genetic acronyms: what does "son of sevenless" mean and what does it do?). Are these lists available by Internet? Thanks for any help, Harold V. Taylor, Dipl. Biochem. Physiol. Chem. Institut University of Tuebingen Tuebingen, Germany phone: 7071-29-3353 e-mail: cbei01@commlink.zdv.uni-tuebinge.de Harold V. Taylor, Dipl. Biochem. Physiol. Chem. Institut University of Tuebingen, Germany e-mail: cbei01@mailserv.zdv.uni-tuebinge.de From owner-rapd@net.bio.net Thu Dec 08 22:00:00 1994 Path: biosci!UNAMVM1.DGSCA.UNAM.MX!carvalho From: carvalho@UNAMVM1.DGSCA.UNAM.MX (Carvalho Torres Alexandro cassio-UACPYP) Newsgroups: bionet.molbio.rapd Subject: primers Date: 8 Dec 1994 09:39:58 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: Dear netters, We have a request: Who have different fingerprint patterns, using the same primers, but with distint lots of primers? What is it the posible main cause of this error? What is it he best company to do primers in our opinion? Thanks a lot. Alexandro Carvalho INNSZ ^ * * * * 0 * * 0 0 * *------@@-----* @@ @@ @@ From owner-rapd@net.bio.net Thu Dec 08 22:00:00 1994 Path: biosci!LIFSCI.SDSU.EDU!MCCLELLAND From: MCCLELLAND@LIFSCI.SDSU.EDU Newsgroups: bionet.molbio.rapd Subject: postdoc Date: 8 Dec 1994 19:02:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <941208190115.2020073e@LIFSCI.SDSU.EDU> NNTP-Posting-Host: net.bio.net Please post Postdoctoral Position DNA and RNA fingerprinting in mammalian cells. Available any time in the next year. Please submit curriculum vitae to: Michael McClelland, California Institute of Biological Research, 11099 North Torrey Pines Road, La Jolla, CA 92037. FAX 619 535 5472. References: (1) Mclelland et al., 1994. Interactions among regulators of RNA abundance characterized using RNA fingerprinting by arbitrarily primed PCR. Nucleic Acids Res. 22:4419. Ionov et al., 1993. Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis. Nature, 363:558-561; Peinado et al., 1992. Isolation and characterization of allelic losses and gains in colorectal tumors by arbitrarily primed polymerase chain reaction. Proc. Natl. Acad. Sci. USA, 89:10065; Welsh et al., 1992. Arbitrarily primed PCR fingerprinting of RNA. Nucleic Acids Res. 20:4965-4970; Ralph et al., 1993. RNA fingerprinting using arbitrarily primed PCR identifies differentially expressed genes in Mink lung (Mu1Lv) cells growth arrested by transforming growth factor-beta1. Proc. Natl. Acad. Sci. USA, 90 :10710-10714; Wong, et al., 1993. Identification of differentially expressed RNA in human ovarian carcinoma cells by arbitrarily primed PCR fingerprinting of total RNAs. Int. J. Oncology 3:13-17; Wong et al., 1994. Stress-inducible gene of Salmonella typhimurium identified by arbitrarily primed PCR of RNA. Proc. Natl. Acad. Sci. U.S.A. 91: 639-643. CIBR is a not-for-profit basic molecular biology research institute From owner-rapd@net.bio.net Thu Dec 08 22:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!munnari.oz.au!news.unimelb.edu.au!ucsvc.ucs.unimelb.edu.au!lugb!zoodr@zoo.latrobe.edu.au Newsgroups: bionet.molbio.rapd Subject: Re: "FSCP" Help! Message-ID: <1994Dec9.044155.4803@lugb.latrobe.edu.au> From: zoodr@zoo.latrobe.edu.au (Dave Runciman) Date: Fri, 9 Dec 1994 04:41:55 GMT Sender: zoodr@zoo.latrobe.edu.au@zoom.zoo.latrobe.edu.au. References: <199411291651.IAA00612@net.bio.net> Organization: Schools of Zoology & Genetics, La Trobe University, Australia. X-Posted-From: InterNews 1.0@lugb.latrobe.edu.au. Lines: 13 I am not familiar with 'FSCP', however the reviewer may have meant 'SSCP' or 'Single-Strand Conformational Polymorphism' (perhaps a typo). The original article describing this technique is Orita et al. (1989) Proceedings of the National Academy of Sciences of the USA, 86, 2766-2770. There have been plenty of publications since describing variations of this method, particularly in recent issues of Nucleic Acids Research and Biotechniques. Hope this is helpful, David Runciman, Schools of Zoology/Genetics and Human Variation, La Trobe University, Australia. (zoodr@zoo.latrobe.edu.au) From owner-rapd@net.bio.net Thu Dec 08 22:00:00 1994 Path: biosci!LIFSCI.SDSU.EDU!MCCLELLAND From: MCCLELLAND@LIFSCI.SDSU.EDU Newsgroups: bionet.molbio.rapd Subject: (none) Date: 8 Dec 1994 18:49:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <941208184757.2020073e@LIFSCI.SDSU.EDU> NNTP-Posting-Host: net.bio.net subscribe rapd From owner-rapd@net.bio.net Sat Dec 10 22:00:00 1994 Path: biosci!DOC.MEDIC.MIE-U.AC.JP!h-makoto From: h-makoto@DOC.MEDIC.MIE-U.AC.JP (Makoto Hirai) Newsgroups: bionet.molbio.rapd Subject: can I get any information Date: 11 Dec 1994 07:36:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9412111540.AA25411@doc.medic.mie-u.ac.jp> NNTP-Posting-Host: net.bio.net Please send me information concerning RAPD. From owner-rapd@net.bio.net Mon Dec 12 22:00:00 1994 Newsgroups: bionet.molbio.rapd Path: biosci!agate!spool.mu.edu!uwm.edu!rutgers!rockyd!psinntp!psinntp!psinntp!hk.super.net!news.ust.hk!hpg30a.csc.cuhk.hk!hkpu01.hkp.hk!hkpcc.hkp.hk!bcdlee From: bcdlee@hkpcc.hkp.hk Subject: Primers Message-ID: <1994Dec12.142513.1@hkpcc.hkp.hk> Lines: 17 Sender: usenet@hkpu01.hkp.hk (Usenet Account) Nntp-Posting-Host: hkpv17 Organization: Hong Kong Polytechnic University Date: Mon, 12 Dec 1994 06:25:12 GMT Subject: Primers Running out of funds, but need RAPD primer sets. Wish to know if anyone has them in excess and willing to donate them to this researcher in Hong Kong? Much appreciated! Daniel Lee Hong Kong Polytechnic University Department of Applied Biology and Chemical Technology Hung Hom Kowloon Hong Kong Tel (852) 766 5615 Fax (852) 364 9932 BCDLEE@HKPCC.HKP.HK From owner-rapd@net.bio.net Mon Dec 12 22:00:00 1994 Path: biosci!PBS.DFO.CA!"PBS::NELSONJ" From: "PBS::NELSONJ"@PBS.DFO.CA Newsgroups: bionet.molbio.rapd Subject: minisatellites Date: 13 Dec 1994 13:54:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <941213135734.20e04bb6@PBS.DFO.CA> NNTP-Posting-Host: net.bio.net Hi Does anyone know of a case where a minisatellite greater than 1 kb in length has been amplified by PCR and used in population analysis without the use of Southern blotting after it's amplification? John Nelson nelsonj@pbs.dfo.ca PS The reply function on your machine may not work for my address From owner-rapd@net.bio.net Mon Dec 12 22:00:00 1994 Path: biosci!DOC.MEDIC.MIE-U.AC.JP!h-makoto From: h-makoto@DOC.MEDIC.MIE-U.AC.JP (Makoto Hirai) Newsgroups: bionet.molbio.rapd Subject: differential display Date: 13 Dec 1994 02:08:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <9412131012.AA28030@doc.medic.mie-u.ac.jp> NNTP-Posting-Host: net.bio.net Dear Netter I do my research with the differential display(Liang.,1992 Science) to find hormone inducible gene(hormone receptor gene) at early response. But my sequencing gel pattern is bad(only low size and small number of PCR fragment was amplified.....). So.I've been trying to improve my PCR condition. My question is that; How many primer is needed to find specific band (on average). Please give me any advice about this techniq. Thank you in advance for your kindness. Makoto Hirai From owner-rapd@net.bio.net Tue Dec 13 22:00:00 1994 Path: biosci!agate!howland.reston.ans.net!torn!news.unb.ca!coranto.ucs.mun.ca!leif!cmcgowan From: cmcgowan@kean.ucs.mun.ca Newsgroups: bionet.molbio.rapd Subject: subscribe Date: 14 Dec 94 11:50:46 -0230 Organization: Memorial University. St.John's Nfld, Canada Lines: 1 Message-ID: <1994Dec14.115046.1@leif> NNTP-Posting-Host: leif.ucs.mun.ca subscribe From owner-rapd@net.bio.net Tue Dec 13 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!grapevine.lcs.mit.edu!uhog.mit.edu!europa.eng.gtefsd.com!gatech!cs.utk.edu!martha.utk.edu!utkvx.utk.edu!linn From: linn@utkvx.utk.edu (Linn, Charles Ernest) Newsgroups: bionet.molbio.rapd Subject: Re: differential display Date: 14 Dec 1994 14:11 EST Organization: University of Tennessee Lines: 60 Distribution: world Message-ID: <14DEC199414111845@utkvx.utk.edu> References: <9412131012.AA28030@doc.medic.mie-u.ac.jp> NNTP-Posting-Host: utkvx1.utk.edu News-Software: VAX/VMS VNEWS 1.41 In article <9412131012.AA28030@doc.medic.mie-u.ac.jp>, h-makoto@DOC.MEDIC.MIE-U.AC.JP (Makoto Hirai) writes... >Dear Netter > I do my research with the differential display(Liang.,1992 Science) to >find hormone inducible gene(hormone receptor gene) at early response. But >my sequencing gel pattern is bad(only low size and small number of PCR >fragment was amplified.....). So.I've been trying to improve my PCR >condition. > My question is that; How many primer is needed to find specific band (on >average). Please give me any advice about this techniq. > Thank you in advance for your kindness. > > Makoto Hirai > Here's some references you may find helpful: Wong K K, and McClelland M, "Stress-inducible gene of Salmonella typhimurium identified by arbitrarily primed PCR of RNA", Proc. Natl. Acad. Sci., 1994, 91:639-643 Caetano-Anolles G and Gresshoff P M, "DNA Amplification Fingerprinting Using Arbitrary Mini-hairpin Oligonucleotide Primers", Bio/Technology, 1994, 12:619-623 Bassam J, Caetano-Anolles G, and Gresshoff P M, "DNA amplification fingerprinting of bacteria", Appl. Microbiol. Biotechnol., 1992, 38:70-76 Caetano-Anolles G, Bassam BJ, and Gresshoff PM, "Buffer Components Tailor DNA Amplification with Arbitrary Primers", PCR Methods and Applications, 1994, 4:59-61 Caetano-Anolles G, Bassam BJ, and Gresshoff PM, "Primer-template interactions during DNA amplification fingerprinting with single arbitrary oligonucleotides", Mol. Gen. Genet., 1992, 235:157-165 Another resource for examining primers is the freeware Mac program AMPLIFY by W.R. Engels. Although not written specificly for RAPDs, it allows you to examine possible band patterns produced from a known template. (You can use a known sequence that you suspect is similar to that you are trying to amplify.) Primer selection for RAPDs, especially for prokaryotes seems to be simply running reactions with different primers until you get something that works. (I'd appreciate any logical feedback on that statement.) However, there are a few approaches to limiting the number of primers that you have to go thru experimentally. First, the use of 5-mers attached to a "mini-hairpin" as described by Caetano-Anoles, may reduce the total number of possibilities to around 1000. Secondly, and the approach that were using here is to examine known sequences that are similar or at least in the same group of things that we are interested in. I use a program I have written to determine primers which would (theoretically) produce the maximum number of bands. I then examine these primers using the program Amplify to determine those primers which would provide the best results. (I ended up with 5 primers to screen, all 5 of which provided differential results!) Third, many people have had good results just comparing every primer that they can get there hands on. (Sequencing primers, probes, etc.) Well, hope this information helps. Keep me informed on your results or if you come up with other approaches to primer selection for RAPDs. Charles From owner-rapd@net.bio.net Wed Dec 14 22:00:00 1994 Newsgroups: bionet.molbio.rapd Path: biosci!rutgers!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!newsfeed.ksu.ksu.edu!moe.ksu.ksu.edu!bubba.ucc.okstate.edu!osuunx.ucc.okstate.edu!David.Demezas From: demed@vms.ucc.okstate.edu. (David Demezas) Subject: RAPD/bacteria Message-ID: X-Posted-From: InterNews 1.0@osuunx.ucc.okstate.edu. Lines: 11 Sender: -Not-Authenticated-[3075] Nntp-Posting-Host: lse421-1.microb.okstate.edu Organization: Oklahoma State University, Stillwater OK Date: Wed, 14 Dec 1994 23:26:04 GMT Xdisclaimer: No attempt was made to authenticate the sender's name. I wish to use RAPD to fingerprint fluorescent pseudomonads. I have been using REP-PCR (ala Versalovic) and have had great success. I now want to expand the number of primers to exam more of the genome. Does anyone have any experience with RAPD analysis of pseudomonads or bacteria with similar G+C content and if so, do you have any suggestions on a source of random primers. I know that there are several sources of primers, I have looked into two, Operon and Genosys. Operon supplies random primers with a G+C content of between 60 and 70%. Genosys' primers are specifically 60 or 70 or 80%, whatever you want. Is it wise to initiate studies using a collection of primers with a single percentage or a range of percentages? I would be greatful for any comments/suggestions. From owner-rapd@net.bio.net Wed Dec 14 22:00:00 1994 Newsgroups: bionet.molbio.rapd Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.ksu.ksu.edu!moe.ksu.ksu.edu!bubba.ucc.okstate.edu!osuunx.ucc.okstate.edu!David.Demezas From: demed@vms.ucc.okstate.edu. (David Demezas) Subject: RAPD/bacteria Message-ID: X-Posted-From: InterNews 1.0@osuunx.ucc.okstate.edu. Lines: 11 Sender: -Not-Authenticated-[3075] Nntp-Posting-Host: lse421-1.microb.okstate.edu Organization: Oklahoma State University, Stillwater OK Date: Wed, 14 Dec 1994 23:23:40 GMT Xdisclaimer: No attempt was made to authenticate the sender's name. I wish to use RAPD to fingerprint fluorescent pseudomonads. I have been using REP-PCR (ala Versalovic) and have had great success. I now want to expand the number of primers to exam more of the genome. Does anyone have any experience with RAPD analysis of pseudomonads or bacteria with similar G+C content and if so, do you have any suggestions on a source of random primers. I know that there are several sources of primers, I have looked into two, Operon and Genosys. Operon supplies random primers with a G+C content of between 60 and 70%. Genosys' primers are specifically 60 or 70 or 80%, whatever you want. Is it wise to initiate studies using a collection of primers with a single percentage or a range of percentages? I would be greatful for any comments/suggestions. From owner-rapd@net.bio.net Wed Dec 14 22:00:00 1994 Newsgroups: bionet.molbio.rapd Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.ksu.ksu.edu!moe.ksu.ksu.edu!bubba.ucc.okstate.edu!osuunx.ucc.okstate.edu!David.Demezas From: demed@vms.ucc.okstate.edu. (David Demezas) Subject: RAPD/bacteria Message-ID: X-Posted-From: InterNews 1.0@osuunx.ucc.okstate.edu. Lines: 11 Sender: -Not-Authenticated-[3075] Nntp-Posting-Host: lse421-1.microb.okstate.edu Organization: Oklahoma State University, Stillwater OK Date: Wed, 14 Dec 1994 23:25:01 GMT Xdisclaimer: No attempt was made to authenticate the sender's name. I wish to use RAPD to fingerprint fluorescent pseudomonads. I have been using REP-PCR (ala Versalovic) and have had great success. I now want to expand the number of primers to exam more of the genome. Does anyone have any experience with RAPD analysis of pseudomonads or bacteria with similar G+C content and if so, do you have any suggestions on a source of random primers. I know that there are several sources of primers, I have looked into two, Operon and Genosys. Operon supplies random primers with a G+C content of between 60 and 70%. Genosys' primers are specifically 60 or 70 or 80%, whatever you want. Is it wise to initiate studies using a collection of primers with a single percentage or a range of percentages? I would be greatful for any comments/suggestions. From owner-rapd@net.bio.net Thu Dec 15 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!gatech!cs.utk.edu!martha.utk.edu!utkvx.utk.edu!linn From: linn@utkvx.utk.edu (Linn, Charles Ernest) Newsgroups: bionet.molbio.rapd Subject: Re: RAPD/bacteria Date: 15 Dec 1994 18:39 EST Organization: University of Tennessee Lines: 34 Distribution: world Message-ID: <15DEC199418391647@utkvx.utk.edu> References: NNTP-Posting-Host: utkvx2.utk.edu News-Software: VAX/VMS VNEWS 1.41 In article , demed@vms.ucc.okstate.edu. (David Demezas) writes... >I wish to use RAPD to fingerprint fluorescent pseudomonads. I have >been using REP-PCR (ala Versalovic) and have had great success. I now >want to expand the number of primers to exam more of the genome. Does >anyone have any experience with RAPD analysis of pseudomonads or >bacteria with similar G+C content and if so, do you have any >suggestions on a source of random primers. I know that there are >several sources of primers, I have looked into two, Operon and Genosys. > Operon supplies random primers with a G+C content of between 60 and >70%. Genosys' primers are specifically 60 or 70 or 80%, whatever you >want. Is it wise to initiate studies using a collection of primers >with a single percentage or a range of percentages? I would be >greatful for any comments/suggestions. We've used the Genosys primers with a relative degree of success (60, 70, and 80). 70 and 80 percent seemed to work the best. Strange thing though was that our results were rather poor and not at all what we expected with some known sequences! When we got our DNA synthisizer and produced these _same primers_ we started getting fantastic fingerprints. Although we're still checking these against predictions for known sequences, it looks pretty good thusfar. May be a good idea to use the range of primers, _and_ check each against known sequences until you get consistent results. We have been working with several Pseudomonas sp. (though not exclusively) and they seem to be straightforward to work with in pure culture. Can be a pain when you're getting them from an environmental source though. BTW, does _anyone_ have any suggestions for good strategies for RAPD primer selection? I outlined a little about what I'm doing in my last message to this group, but would appreciate any ideas for other strategies other than going through every primer possible. Chuck From owner-rapd@net.bio.net Sun Dec 18 22:00:00 1994 Path: biosci!YVAX.BYU.EDU!FARMERJ From: FARMERJ@YVAX.BYU.EDU Newsgroups: bionet.molbio.rapd Subject: Re: storage and trouble shooting Date: 19 Dec 1994 08:40:20 -0800 Organization: Brigham Young University Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HKTN7ZYE5E8ZJ0D0@yvax.byu.edu> NNTP-Posting-Host: net.bio.net I am still using some primers that were synthesized in October 1990. They work very well. They are stored in water at -20 C at the same concentration at which they came off the synthesizer. Periodically, I dilute a fraction to 10 micromolar and use that until it is gone (typically a few months). The dilutions are also into water and stored at -20 C. My experience so far is that when RAPD's start to weaken (less amplification), it is either the template that has gone bad or (more often, in my case) the Taq polymerase seems to be bad. Usually, opening a new vial of polymerase solves the problem. Templates go bad at 4 C in my experience. However, since I work on single Drosophila, I use crude extracts for RAPD (grind with proteinase K, incubate at 65 C, dilute with TE, boil for 10 minutes). These preparations seem to be very stable (for at least a couple of years) at -20 C. However, even highly purified DNA goes bad, especially when diluted, at 4 C. I hope that this is helpful. James Farmer Dept. of Zoology, Brigham Young University, Provo, Utah 84602, USA farmerj@yvax.byu.edu From owner-rapd@net.bio.net Sun Dec 18 22:00:00 1994 Path: biosci!ACS.TAMU.EDU!J0V8959 From: J0V8959@ACS.TAMU.EDU Newsgroups: bionet.molbio.rapd Subject: storage, trouble shooting Date: 18 Dec 1994 19:13:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <941218211333.220841b@ACS.TAMU.EDU> NNTP-Posting-Host: net.bio.net Two questions: 1) Does anyone have any experience with the "shelf life" of rapd primers? We are still using some of the original (1991) UBC primers, but the results don't seem to be as good as they used to be. They are mostly stored in water at 5ng/ul at -20C. 2) Does anyone have a good trouble shooting strategy? I have thought of freezing aliquot's of all reagents at -80C when the reaction is working great, and use these to track down the offender, when problems show up. I have never implemented this strategy, because it is a fair amount of work, but I still like the idea. Are there simpler ways to accomplish the same thing? J.P. van Buijtenen Dept of Forest Science Texas A&M University From owner-rapd@net.bio.net Sun Dec 18 22:00:00 1994 Path: biosci!ABRSLE.AGR.CA!HACHEY From: HACHEY@ABRSLE.AGR.CA (john hachey) Newsgroups: bionet.molbio.rapd Subject: re:storage,trouble shooting Date: 18 Dec 1994 21:21:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 124 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HKT3MB6Y020016ZB@GW.AGR.CA> NNTP-Posting-Host: net.bio.net Date sent: 18-DEC-1994 22:19:13 > >Two questions: >1) Does anyone have any experience with the "shelf life" of rapd primers? > We are still using some of the original (1991) UBC primers, but the > results don't seem to be as good as they used to be. They are mostly > stored in water at 5ng/ul at -20C. A recent note on another network may be of interest here From: OTTGW::IN%"pnh@fcs260c2.ncifcrf.gov" Date: 6-DEC-1994 11:58:33 Description: Primer stocks that fail to PCR over time Earlier I wrote: : Some netters have noticed a loss in the ability to amplify DNA by the : polymerase chain reaction when using the same lot of primers over a period of : time. On occasion, the failure of PCR amplification was directly linked : to the primer stock going belly up for unknown reasons. : : PCR primers initially worked shortly after being purchased, but after storage : for several months at -20 C as diluted stocks in water, these same primers : failed to give the same amount of amplified product in identical reactions. : Primers going bad also produced unreadable results in automated DNA sequencing : reactions using the dye terminator chemistry combined with cycle sequencing and : subsequent analysis on the ABI model 373A sequencer under the same conditions : that previously gave good results. If new primers were purchased, the reactions : gave good results and acted as they did prior to storage at - 20 C. ================================================================================ This came from Andre-Denis Wright (awright@uoguelph.ca): | I read with interest your posting on primer stocks. This is the | same problem we've been experiencing in our laboratory. This summer I was | getting great PCR results, but for the past two months, I have not been | able to get any amplifications. We ordered new primers, and i am using | them with the other reagents i used with the old primers and now i am | getting great amplifications again. We have been storing our | concentrated primers, like everyone else, at -20 C. I can't figure this | out. : Dear Andre-Denis: : : The best response for explaining this problem was from Roger Aeschbacher. : : Roger Aeschbacher (teschba@fmi.ch) suggested that some primer : sets cause PCR failure by nonspecific priming because they are more likely to : form primer-dimers or intrastrand secondary structures such as hairpins only : after undergoing multiple cycles of warming and slow freezing. This would not : happen to all sets of primers, explaining the reason why some go bad whilst : others remain functional. It also explains why some primers seem to work after : a period of storage and others purchased from the same company on the same date : do not. : : While Hot Start PCR may help reduce non-specific annealing to template DNA, : denaturing the bad primer stock at 95 C to 100 C for 5 minutes and then quickly : cooling it in a dry ice/ethanol bath or liquid nitrogen may help to restore : these primers to their former selves. | Dear Dr. Hengen, | I took your advice about the primers and boiled two aliquots (100ul | ea) at 100 C for 5 min before freezing quickly with liquid N2. The next | day i thawed one of the samples and used it it a PCR reaction and | VOILA!!! they worked. Thanks for the advice! | | Andre-Denis Ahhhh. Excellent result! This topic will be discussed in my monthly column in the January 1995 issue of TIBS. @article{Hengen1995Jantibs, author = "P. N. Hengen", title = "Methods and Reagents - Wayward PCR primers", journal = "Trends in Biochemical Sciences", volume = "20", number = "1", pages = "???-???", month = "January", year = "1995"} -Paul. ******************************************************************************* * Paul N. Hengen, Ph.D. /--------------------------/* * National Cancer Institute |Internet: pnh@ncifcrf.gov |* * Laboratory of Mathematical Biology | Phone: (301) 846-5581 |* * Frederick Cancer Research and Development Center| FAX: (301) 846-5598 |* * Frederick, Maryland 21702-1201 USA /--------------------------/* * - - - Methods FAQ list -> http://www.ncifcrf.gov:2001/~pnh/info.html - - - * ******************************************************************************* Return-path: Received: from net.bio.net by GW.AGR.CA (PMDF #3062 ) id <01HKBPX2DM2O000A3C@GW.AGR.CA>; Tue, 6 Dec 1994 13:43:21 EST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id KAA00856 for methods-and-reagents-list; Tue, 6 Dec 1994 10:37:07 -0800 Received: (from news@localhost) by net.bio.net (8.6.9/8.6.6) id KAA00852 for bionet-methods-and-reagents-arpanet; Tue, 6 Dec 1994 10:37:06 -0800 Date: 06 Dec 1994 18:26:03 +0000 (GMT) From: pnh@fcs260c2.ncifcrf.gov (Paul N Hengen) Subject: Primer stocks that fail to PCR over time Sender: Paul.N.Hengen@net.bio.net To: methods-and-reagents@net.bio.net Message-id: Content-transfer-encoding: 7BIT Nntp-Posting-Host: fcs260c2.ncifcrf.gov ---------------------------------------------------------------------------- | John E. Hachey * Telephone : (403)327-4561 | | Agriculture Canada * Fax : (403)382-3156 | | Research Station * Email : hachey@abrsle.agr.ca | | P.O. Box 3000, Main * | | Lethbridge, AB * Everything in Moderation, | | Canada * Including Moderation | | T1J 4B1 * | |****************************************************************************| | DISCLAIMER : BUT THEN AGAIN, I LIKED HEE HAW | | | ---------------------------------------------------------------------------- From owner-rapd@net.bio.net Mon Dec 19 22:00:00 1994 Path: biosci!MBRSWI.AGR.CA!GHUMPHREYS From: GHUMPHREYS@MBRSWI.AGR.CA Newsgroups: bionet.molbio.rapd Subject: Access to Green Genes. Date: 20 Dec 1994 07:18:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HKV2LLLCOI001HR4@GW.AGR.CA> NNTP-Posting-Host: net.bio.net Hello, I would like to get access to the latest information on the barley genome mapping project. I am aware that much of this information is available in Green Genes, the cereal database. I would like to know what is the best way to access Green genes to search for such information. I have full internet access so that is not the problem. I have looked for it on gopher but have not been able to find it. Any suggestions would be appreciated. Gavin Humphreys, PhD Visiting Postdoctoral Fellow Agriculture Canada, Winnipeg Research Station Email: ghumphreys@mbrswi.agr.ca From owner-rapd@net.bio.net Mon Dec 19 22:00:00 1994 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!howland.reston.ans.net!pipex!warwick!bham!bcs152.bham.ac.uk!user From: C.Constantinidou@bham.ac.uk (Chrystala Constantinidou) Newsgroups: bionet.molbio.rapd Subject: Inosine in PCR primers Date: 20 Dec 1994 12:06:25 GMT Organization: University of Birmingham Lines: 10 Message-ID: NNTP-Posting-Host: bcs152.bham.ac.uk Hello one and all Does anyone have experience of using inosine in a PCR primer. I have read that it is not a good idea to have them too close to the 3' end - has anyone used inosine and how close is too close. What is it about inosine that may make it unsuitable to be directly at the 3' end? Thanks for any help Wig. From owner-rapd@net.bio.net Tue Dec 20 22:00:00 1994 Path: biosci!agate!howland.reston.ans.net!cs.utexas.edu!swrinde!pipex!sunsite.doc.ic.ac.uk!daresbury!imbb1.imbb.forth.gr!imbb1!louis From: louis@myia.imbb.forth.gr (Kitsos Louis) Newsgroups: bionet.molbio.rapd Subject: POSTDOC POSITIONS Followup-To: bionet.molbio.rapd Date: Wed, 21 Dec 94 14:26:48 GMT Organization: IMBB Lines: 13 Message-ID: NNTP-Posting-Host: louiqua.imbb.forth.gr X-Newsreader: VersaTerm Link v1.1.1 INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY FOUNDATION FOR RESEARCH AND TECHNOLOGY, HELLAS HERAKLION, CRETE, GREECE TWO POSTDOCTORAL POSITIONS, FUNDED BY THE MACARTHUR FOUNDATION, ARE AVAILABLE AT THE INSECT MOLECULAR GENETICS GROUP OF THE IMBB. THE RESEARCH TOPICS INCLUDE GENOME ANALYSIS AND MOLOCULAR GENETICS OF THE MALARIA MOSQUITO ANOPHELES GAMBIAE. APPLICANTS SHOULD SEND A CV AND ARRANGE FOR 2 LETTERS OF RECOMMENDATION TO BE SENT TO KITSOS LOUIS: IMBB, FORTH, BOX 1527, 711 10 HERAKLION, CRETE, GREECE FAX: +30-81-231308 E-MAIL: LOUIS@MYIA.IMBB.FORTH.GR From owner-rapd@net.bio.net Sat Dec 24 22:00:00 1994 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ Date: 25 Dec 1994 02:00:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 338 Sender: daemon@net.bio.net Distribution: world Message-ID: <199412251000.CAA27214@net.bio.net> NNTP-Posting-Host: net.bio.net Four important items follow: How to cancel e-mail subscriptions to BIOSCI newsgroups, BIOSCI archive searching, the BIOSCI FAQ, and the BIOSCI User Address Directory form. 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This allows one to retrieve via WAIS or waismail the list of participants in a particular group. For example: comment: ARABIDOPSIS PLANT-BIOLOGY BIONEWS On the comment: lines use these names below ---- NOT the USENET names below MAILING LIST NAME USENET Newsgroup Name ----------------- --------------------- ACEDB-SOFT bionet.software.acedb AGEING bionet.molbio.ageing AGROFORESTRY bionet.agroforestry ARABIDOPSIS bionet.genome.arabidopsis ASCB bionet.prof-society.ascb BIOCAN bionet.prof-society.cfbs BIOFORUM bionet.general BIO-INFORMATION-THEORY bionet.info-theory BIONAUTS bionet.users.addresses BIONEWS bionet.announce BIO-JOURNALS bionet.journals.contents BIO-MATRIX bionet.molbio.bio-matrix BIOPHYSICAL-SOCIETY bionet.prof-society.biophysics BIOPHYSICS bionet.biophysics BIO-SOFTWARE bionet.software BIOTHERMOKINETICS bionet.metabolic-reg BIO-WWW bionet.software.www CARDIOVASCULAR-RESEARCH bionet.biology.cardiovascular CELEGANS bionet.celegans CELL-BIOLOGY bionet.cellbiol CHLAMYDOMONAS bionet.chlamydomonas CHROMOSOMES bionet.genome.chromosomes COMPUTATIONAL-BIOLOGY bionet.biology.computational CSM bionet.prof-society.csm CYTONET bionet.cellbiol.cytonet DROSOPHILA bionet.drosophila EMBL-DATABANK bionet.molbio.embldatabank EMF-BIO bionet.emf-bio EMPLOYMENT bionet.jobs EMPLOYMENT-WANTED bionet.jobs.wanted FASEB bionet.prof-society.faseb GDB bionet.molbio.gdb GENBANK-BB bionet.molbio.genbank GENETIC-LINKAGE bionet.molbio.gene-linkage GRASSES-SCIENCE bionet.biology.grasses HIV-MOLECULAR-BIOLOGY bionet.molbio.hiv HUMAN-GENOME-PROGRAM bionet.molbio.genome-program IMMUNOLOGY bionet.immunology INFO-GCG bionet.software.gcg JOURNAL-NOTES bionet.journals.note METHODS-AND-REAGENTS bionet.molbio.methds-reagnts MICROBIOLOGY bionet.microbiology MOLECULAR-EVOLUTION bionet.molbio.evolution MOLECULAR-MODELLING bionet.molec-model MOLLUSC-MOLECULAR-NEWS bionet.molbio.molluscs MYCOLOGY bionet.mycology NEUROSCIENCE bionet.neuroscience N2-FIXATION bionet.biology.n2-fixation PARASITOLOGY bionet.parasitology PHOTOSYNTHESIS bionet.photosynthesis PLANT-BIOLOGY bionet.plants POPULATION-BIOLOGY bionet.population-bio PROTEIN-ANALYSIS bionet.molbio.proteins PROTEIN-CRYSTALLOGRAPHY bionet.xtallography PROTISTA bionet.protista RAPD bionet.molbio.rapd SCIENCE-RESOURCES bionet.sci-resources STADEN bionet.software.staden STRUCTURAL-NMR bionet.structural-nmr TROPICAL-BIOLOGY bionet.biology.tropical URODELES bionet.organisms.urodeles VIROLOGY bionet.virology WOMEN-IN-BIOLOGY bionet.women-in-bio YEAST bionet.molbio.yeast ZBRAFISH bionet.organisms.zebrafish Listing newsgroups on the comment: line is optional, of course. Thanks again for your cooperation! --------------- please cut here and return portion below --------------- New information or Update to old record (enter N or U): date (DD-MM-YY): first name: middle initial: family name: job title: e-mail address: e-mail network: phone number: FAX number: institution: address1: address2: address3: city: state/province: country: postal code: research interest: research interest: comment: comment: comment: comment: comment: