From owner-rapd@net.bio.net Sun Apr 02 23:00:00 1995 Path: biosci!DEAKIN.EDU.AU!huangbx From: huangbx@DEAKIN.EDU.AU Newsgroups: bionet.molbio.rapd Subject: HELP----Tricine buffer recipe for PCR? Date: 3 Apr 1995 05:21:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <199504031220.WAA26873@hestia.ccs.deakin.edu.au> NNTP-Posting-Host: net.bio.net Dear netters, I have tried very hard to get one of my abalone minisatellite DNA repeat work. However, the PCR results are still very faint up to now. I would like to try another buffer. About one month ago, people discussed Tricine PCR buffer on the net. The recipe I quickly recorded as follows: 1X 300mM Tricine (pH8.4) 50mM 2-mercaptoethanol 17ug/ml BSA Questions: 1. As recorded by hand writing, is above buffer recipe in right ratio? 2. Is it suitable for Taq DNA polymerase from Perkin Elmer? Many thanks. Bixing Huang huangbx@deakin.edu.au Deakin University of Australia From owner-rapd@net.bio.net Mon Apr 03 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!torn!ccshst05.cs.uoguelph.ca!ccshst01.cs.uoguelph.ca!jpopi From: jpopi@uoguelph.ca (Jon Popi) Newsgroups: bionet.molbio.rapd Subject: Bootstrap procedure in maize diversity analyses Date: 4 Apr 1995 14:17:15 GMT Organization: University of Guelph Lines: 22 Message-ID: <3lrkdb$m8j@ccshst05.cs.uoguelph.ca> NNTP-Posting-Host: ccshst01.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] I would like to evaluate the diversity existing within and among sixteen maize populations by using RAPD analyses. What is puzzling me is the number of plants that should be analyzed per population (of course, I would like to analyze the smallest number of plants that would provide reliable results). In order to find this out, I have run RAPD analyses with ten primers on 100 plants from one of the populations (presumably the least diverse), and I would like to apply the bootstrap procedure in order to find the standard error for samples of different sizes. Does anybody know of a computer program that would perform all three steps of the Monte Carlo algorithm for the bootstrap procedure (i.e. the sampling, computation of the distances and the calculation of the standard error)? Thank you very much in advance! Jon Popi Crop Science Department University of Guelph Guelph, Ontario Canada jpopi@uoguelph.ca From owner-rapd@net.bio.net Mon Apr 03 23:00:00 1995 Path: biosci!CSHL.ORG!lodhi From: lodhi@CSHL.ORG (Muhammad Lodhi) Newsgroups: bionet.molbio.rapd Subject: Dario Grattapaglias' address in Brazil ?? Date: 4 Apr 1995 11:32:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Does anyone know Dario Grattapaglia's present address or affiliation in Brazil. He worked at North Carolina State University?? for his Ph.D. Any help will be appreciated Muhamamd A. Lodhi Cold Spring Harbor Lab From owner-rapd@net.bio.net Tue Apr 04 23:00:00 1995 Newsgroups: bionet.molbio.rapd Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!ix.netcom.com!netcom.com!bks From: bks@netcom.com (Bradley K. Sherman) Subject: Re: Dario Grattapaglias' address in Brazil ?? Message-ID: Organization: Dendrome, A Genome Database for Forest Trees References: Date: Wed, 5 Apr 1995 04:54:22 GMT Lines: 34 Sender: bks@netcom11.netcom.com In article lodhi@CSHL.ORG (Muhammad Lodhi) writes: >Does anyone know Dario Grattapaglia's present address or affiliation in >Brazil. He worked at North Carolina State University?? for his Ph.D. Any >help will be appreciated I have just mailed this information to Dr. Lodhi. The Dendrome project at the Institute of Forest Genetics maintains a directory of scientists studying the molecular biology of forest trees. This directory is WAIS indexed (i.e. searchable on any text) and is available by gopher or WWW at: gopher://s27w007.pswfs.gov/ gopher://probe.nalusda.gov:7508/ http://s27w007.pswfs.gov/ We have just done a mass mailing under the tutelage of F. Thomas Ledig to update the IUFRO World Directory of Forest Geneticists and Tree Breeders. Almost all of the resulting data has been put online. We also have a list of commercially available 10-mers sorted by sequence. And we are activily seeking to add data from RAPD mapping experiments in forest species to our WWW, gopher and ACEDB databases. Please email me or consult the services listed above for more information. --bks Bradley K. Sherman | Institute of Forest Genetics bks@s27w007.pswfs.gov | P.O. Box 245 510-559-6437 FAX:510-559-6440 | Berkeley, CA 94701 USA Dendrome Project From owner-rapd@net.bio.net Thu Apr 06 23:00:00 1995 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!agate!howland.reston.ans.net!news.sprintlink.net!sunserver.insinc.net!news.Direct.CA!scipio.cyberstore.ca!vanbc.wimsey.com!unixg.ubc.ca!news.bc.net!rover.ucs.ualberta.ca!tribune.usask.ca!skyfox.usask.ca!LEES From: LEES@skyfox.usask.ca (Lee, Sun ) Newsgroups: bionet.molbio.rapd Subject: looking for phone # Date: 7 Apr 1995 13:13:12 GMT Organization: University of Saskatchewan Lines: 5 Message-ID: <3m3dp8$q63@tribune.usask.ca> NNTP-Posting-Host: sask.usask.ca X-Newsreader: TIN [version 1.2 PL2v [VAX/VMS]] Does anyone know Dr. Felsenstein's phone number? Thanks, lee From owner-rapd@net.bio.net Thu Apr 06 23:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!cs.utexas.edu!news.sprintlink.net!uunet!boulder!stormo From: stormo@beagle.Colorado.EDU (Gary Stormo) Newsgroups: bionet.molbio.rapd Subject: Re: looking for phone # Date: 7 Apr 1995 21:33:25 GMT Organization: University of Colorado at Boulder Lines: 22 Message-ID: <3m4b35$b2r@lace.Colorado.EDU> References: <3m3dp8$q63@tribune.usask.ca> NNTP-Posting-Host: beagle.colorado.edu LEES@skyfox.usask.ca (Lee, Sun ) writes: >Does anyone know Dr. Felsenstein's phone number? >Thanks, lee Joe Felsenstein Dept. of Genetics SK-50 Univ. of Washington Seattle WA 98195 phone: 206-543-0150 JOE@GENETICS.WASHINGTON.EDU I believe you can also get the PHYLIP package via anonymous ftp from the same machine. -- Gary Stormo | MCD Biology | Keep in mind that to the advertising industry, Univ. of Colorado | every day is April Fool's Day. Boulder, CO 80309 | From owner-rapd@net.bio.net Thu Apr 06 23:00:00 1995 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!agate!howland.reston.ans.net!news.sprintlink.net!sunserver.insinc.net!news.Direct.CA!scipio.cyberstore.ca!vanbc.wimsey.com!unixg.ubc.ca!news.bc.net!rover.ucs.ualberta.ca!tribune.usask.ca!skyfox.usask.ca!LEES From: LEES@skyfox.usask.ca (Lee, Sun ) Newsgroups: bionet.molbio.rapd Subject: Phylip program Date: 7 Apr 1995 13:11:54 GMT Organization: University of Saskatchewan Lines: 8 Message-ID: <3m3dmq$q63@tribune.usask.ca> NNTP-Posting-Host: sask.usask.ca X-Newsreader: TIN [version 1.2 PL2v [VAX/VMS]] Hello, wonder where I can find Phylip program in FTP site or introductory document on this? Thanks, Lee From owner-rapd@net.bio.net Fri Apr 07 23:00:00 1995 Newsgroups: bionet.molbio.rapd Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!paladin.american.edu!news.ecn.uoknor.edu!news.uoknor.edu!ns1.nodak.edu!badlands!comstock From: comstock@badlands.NoDak.edu (Clay Comstock) Subject: test1 Sender: usenet@ns1.nodak.edu (Usenet login) Message-ID: Date: Fri, 7 Apr 1995 23:58:11 GMT Nntp-Posting-Host: badlands.nodak.edu Organization: North Dakota Higher Education Computing Network X-Newsreader: TIN [version 1.2 PL2] Lines: 1 From owner-rapd@net.bio.net Mon Apr 10 23:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newsstand.cit.cornell.edu!NewsWatcher!user From: cga3@cornell.edu (Christopher G. Alpha) Newsgroups: bionet.molbio.rapd Subject: anyone score gels w/ image analysis? Followup-To: bionet.molbio.rapd Date: Tue, 11 Apr 1995 16:15:57 +0000 Organization: Cornell University Lines: 9 Sender: cga3@cornell.edu (Verified) Message-ID: NNTP-Posting-Host: 132 Hello all! I am looking for information on the possiblity or lack thereof of scoring RAPDs gels with image analysis software. For example scanning in a gel photo and then importing it into an analysis program. Any thoughts or suggestions on this topic would be greatly appreciated, you may reply here or via Email. Thanx again! CGA From owner-rapd@net.bio.net Mon Apr 10 23:00:00 1995 Path: biosci!greb.ulaval.ca!Christian.Mouton From: Christian.Mouton@greb.ulaval.ca (Christian Mouton) Newsgroups: bionet.molbio.rapd Subject: Re: anyone score gels w/ image analysis? Date: 11 Apr 1995 15:26:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <9504112226.AA02932@hermes.ulaval.ca> NNTP-Posting-Host: net.bio.net >Hello all! I am looking for information on the possiblity or lack thereof >of scoring RAPDs gels with image analysis software. For example scanning >in a gel photo and then importing it into an analysis program. Any >thoughts or suggestions on this topic would be greatly appreciated, you may >reply here or via Email. > Check with P.A.D. Grimont, Institut Pasteur, Paris, who designed "Taxolab softwares": in my hands they perform extremely well and are at affordable price. pgrimont@pasteur.fr (Patrick A. D. Grimont) -----------------------------Christian Mouton----------------------------- Groupe de Recherche en Écologie Buccale tél. (418)656-5872 Faculté de médecine dentaire fax. (418)656-2861 Université Laval Email: Christian.Mouton@greb.ulaval.ca Québec (Québec) G1K 7P4 CANADA From owner-rapd@net.bio.net Mon Apr 10 23:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!pipex!sunsite.doc.ic.ac.uk!rfhsun3.rfhsm.ac.uk!rfhsm.ac.uk!k9mhc From: k9mhc@rfhsm.ac.uk (Mr Vid Mohan-Ram) Newsgroups: bionet.molbio.rapd Subject: scars Date: Tue, 11 Apr 1995 12:02:30 +1000 Organization: Royal Free Hospital School of Medicine Lines: 18 Distribution: world Message-ID: NNTP-Posting-Host: porcupine.rfhsm.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] hi, there were a few postings a little while back regarding scars and how they sidestepped the 'reproducibility' problems of rapds - can anyone please repost these or recommend some references....?....or does it simply entail cloning of the polymorhpic band and desiging specific primers.....?...... another question,.....my statistics and maths background is almost negliable but what kinds of statistical analysis is normally done once you have RAPDS from several subjects.....i mean are there specific equations used to relate band-sharing etc etc?.....hope i'm not wasting band-width but i'm the only one here doing this stuff..............thanks Vid Mohan-Ram Dept. Anatomy and Developmental Biology Royal Free Hospital School of Medicine, Univesity of London, UK. From owner-rapd@net.bio.net Tue Apr 11 23:00:00 1995 Path: biosci!UNIWA.UWA.EDU.AU!mitch From: mitch@UNIWA.UWA.EDU.AU (Michelle Waycott) Newsgroups: bionet.molbio.rapd Subject: image analysis software... Date: 11 Apr 1995 18:42:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: <199504120141.JAA24213@uniwa.uwa.edu.au> NNTP-Posting-Host: net.bio.net Christopher G. Alpha asks about using image analysis software for analysing RADP gels: I use a Mac and so have the advantage of a wonderful program called IMAGE which is public domain program written by Wayne Rasband at NIH and is available by anonymous ftp from zippy.nimh.nih.gov /pub/nih/-image/ I have used both scanned gel photographs and more recently a digital camera hooked up to a Quadra (which is much nicer than the slowness of scanning images!). The nice thing about image, apart from being free is that there have been a range of excellent Macros for various functions, one of which is a gel-plotting macro to give profiles of the lanes on the gels. Another program is NCSA gelreader but I know less about that, but the contact for the developers of this program is softdev@ncsa.uiuc.edu. If you use a PC the scene is less rosy I believe but I am less familiar with that area. good luck gelling Mitch ___________________________________________________________________ /\/\ichelle \/\/aycott Department of Botany mitch@uniwa.uwa.edu.au The University of Western Australia ___________________________________________________________________ *my ideas and comments are my own and do not represent any instiution. From owner-rapd@net.bio.net Tue Apr 11 23:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!news.ksu.ksu.edu!pstpm.pp.ksu.edu!user From: PST@KSU.KSU.Edu (Paul St. Amand) Newsgroups: bionet.molbio.rapd Subject: Re: image analysis software... Date: Wed, 12 Apr 1995 13:55:26 -0500 Organization: USDA/ARS and KSU Agronomy Dept. Lines: 24 Distribution: world Message-ID: References: <199504120141.JAA24213@uniwa.uwa.edu.au> NNTP-Posting-Host: pstpm.pp.ksu.edu X-Newsreader: Value-Added NewsWatcher 2.0b24.0+ In article <199504120141.JAA24213@uniwa.uwa.edu.au>, mitch@UNIWA.UWA.EDU.AU (Michelle Waycott) wrote: PSt> Christopher G. Alpha asks about using image analysis software for analysing PSt> RADP gels: PSt> PSt> I use a Mac and so have the advantage of a wonderful program called IMAGE PSt> which is public domain program written by Wayne Rasband at NIH and is PSt> available by anonymous ftp from zippy.nimh.nih.gov /pub/nih/-image/ PSt> I have used both scanned gel photographs and more recently a digital camera PSt> hooked up to a Quadra (which is much nicer than the slowness of scanning PSt> images!). The nice thing about image, apart from being free is that there PSt> have been a range of excellent Macros for various functions, one of which PSt> is a gel-plotting macro to give profiles of the lanes on the gels. Which digital camera are you using? Does anyone use the Apple QuickTake and how good is it for ethidium bromide stained agarose gels? Thanks. Paul C. St. Amand (PST@KSU.KSU.Edu) USDA Research Geneticist // \\ // \\ // \\ // \\ // 2004 Throckmorton Hall, KSU // | :,\\': | \\ // | :,\\': | \\ // | Manhattan KS 66506-5501\\ // | | // \\ | |\\ // | | // \\ | | \\ // | | (913) 532-6168 \\ | :,\\': | // \\ |:,\\': | // \\ | :,\\': | // Fax (913) 532-5692 \\ // \\ // \\// \\ // \\ // \\ // From owner-rapd@net.bio.net Sat Apr 15 23:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.molbio.rapd Subject: Terminology Date: 16 Apr 1995 08:00:53 -0700 Organization: University of Arkansas Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Would anyone care to recommend an acceptable term for density of markers in a particular genome?? When mapping genomes and you estimate the number of markers used (for example in Bulked Segregant Analysis) this implies, assuming a random distribution of markers, that markers approximate spacing of a particular distance. For example, with 5000 markers on a genome of 1000 Mbp your density of markers is 1000 Mbp/5000=200 Kbp. But does `density of markers' truly fit. One could also use `genome coverage'. Do others out there have favorite expressions?? Just looking for suggestions. //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// From owner-rapd@net.bio.net Sat Apr 15 23:00:00 1995 Path: biosci!ASRR.ARSUSDA.GOV!abartlet From: abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") Newsgroups: bionet.molbio.rapd Subject: Re: anyone score gels w/ image analysis? Date: 15 Apr 1995 21:15:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net I have been using the Scanalytics Scangel V for two years. The software is called RFLPScan. It includes gel analysis and a database with SQL capabilities. The software is about $5000. It can read a TIFF file made from a scanner or from the Scangel V hardware. You can get more information from Scanalytics, CSPI, 40 Linnell Circle, Billerica, MA 01821 800-325-3110 is the number for support, but I think they would send you literateratur also. Good luck! Alan C. Bartlett "GENES-R-US" "ALTERATIONS AVAILABLE!^" "^some restrictions may apply:)" From owner-rapd@net.bio.net Sat Apr 15 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!news From: markb@ravel.zoology.wisc.edu (Mark E. Berres) Newsgroups: bionet.molbio.rapd Subject: Re: anyone score gels w/ image analysis? Followup-To: bionet.molbio.rapd Date: 16 Apr 1995 14:51:44 GMT Organization: The University of Wisconsin, Madison Lines: 74 Distribution: world Message-ID: <3mrau0$16jm@news.doit.wisc.edu> Reply-To: markb@ravel.zoology.wisc.edu NNTP-Posting-Host: ravel.zoology.wisc.edu Keywords: image analysis gel RFLP RAPD sequence AFLP PCR I would strongly suggest DNA/GUI developed by the Washington University Medical School. This program is fantastic! I have used it to score band data from both agarose (RAPDs, RFLPs, PCR products) and acrylamide (AFLPs and sequence) gels. This program was developed under Unix and X11 for Sun and DEC workstations. My image acquisition includes a UV sensitive (for EtBr reqs.) and visible light camera piped into a graphics board on my SS20. Snap the raw image, enhance (rarely is necessary- as long as your optics are good), load into DNA/GUI and away you go! Because of high throughput on agarose gels, we no longer use 667 film for routine records- all images are stored in compressed form and are capable of instant retrieval. Using this software has greatly increased the accuracy of gel reads- not to mention a preventative measure against insanity from reading too many of them. Enough said. DNA/GUI is available from fly.bio. Here is an excerpt from the DNA/GUI readme: The DNA Graphical User Interface (DNA/GUI) is a software program for assisting in the quantitative analysis of 1D-electrophoretic-gel images. The software is written in C for UNIX workstations using the X Window System. This software does NOT include an image acquisition phase. Rather, the software begins with a digital image acquired from any one of a number of sources. An image might be acquired directly from a gel that has been stained with ethidium bromide and transilluminated with ultraviolet light or from an autoradiogram that has been illuminated with visible light. In a typical scenario, an image will be captured by a CCD camera and saved on a small computer attached to the camera; from there, the image would be sent over a local network to a graphics workstation for analysis and review by DNA/GUI. Results may be printed and forwarded to an electronic database. The digital image and quantitative results may be archived for later recall, perhaps to compare with other images. The DNA/GUI software features lane detection and delineation, band detection, and an image-normalization feature to remove or minimize image frowning. Results include lane-by-lane band mobilities and base-pairs. While much of the analysis is fairly automatic, a user is given ample opportunity to edit results so as to make saved information as accurate as possible. The editing is minimized in images of gels which are free of artifacts and for which reasonable attention was given to the gel-formation and electrophoresis experiment. Images of poorly-prepared gels require more editing. This version of the software also has some difficulty resolving very closely-spaced bands. The DNA/GUI system is described in a 1992 issue of BioTechniques: H.A. Drury, K.W. Clark, R.E. Hermes, J.M. Feser, L.J. Thomas, Jr., and H. Donis-Keller. "A Graphical User Interface for Quantitative Imaging and Analysis of Electrophoretic Gels and Autoradiograms", BioTechniques, vol. 12, No. 6 (June, 1992), pp. 892-901. Cheers! Mark -- Mark E. Berres Birge Hall Office, Room 440 Department of Genetics 430 Lincoln Drive Department of Zoology Madison, WI. 53706-1313 University of Wisconsin at Madison Ph: (608)-265-6427 -----BEGIN PGP PUBLIC KEY BLOCK----- Version: 2.6.2 mQCNAy9eAb0AAAEEALiWUAWSEV4g9Glu0Al8VxA62PBkfeSZJC/90guCvZtwgDvC fLDA862kj9Bs5Ae4KwsbYag6QSkG1JqfHzufAFLgTgkt5k68TLfEAsut/WMy8ix3 wVoQAZTbQnOTeGmG2ceA1cYY9pDuzrUAt7vhpBc9pp4rEHXX2Uljqit9IVslAAUR tC1NYXJrIEUuIEJlcnJlcyA8bWFya2JAcmF2ZWwuem9vbG9neS53aXNjLmVkdT4= =IUu8 -----END PGP PUBLIC KEY BLOCK----- From owner-rapd@net.bio.net Sun Apr 16 23:00:00 1995 Path: biosci!agate!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: Newsgroups: bionet.molbio.rapd Subject: RE: Terminology Date: 17 Apr 1995 08:32:37 +0100 Lines: 6 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3mt5il$4io@mserv1.dl.ac.uk> Original-To: rapd@dl.ac.uk I would not recommend the term "genome coverage" to describe the density of markers in a genome. The term is being used for other purposes as well, eg we (the European Drosophila Mapping Consortium) and others use the same term to denote the percentage of the genome that has been unambiguously placed in contigs that build up the physical map. K. Louis From owner-rapd@net.bio.net Mon Apr 17 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.moneng.mei.com!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!news From: markb@ravel.zoology.wisc.edu () Newsgroups: bionet.molbio.rapd Subject: FTP site for DNA/GUI Date: 18 Apr 1995 13:41:57 GMT Organization: The University of Wisconsin, Madison Lines: 28 Distribution: world Message-ID: <3n0fj5$rt6@news.doit.wisc.edu> Reply-To: markb@ravel.zoology.wisc.edu NNTP-Posting-Host: ravel.zoology.wisc.edu To all concerned, I apologize for my mistake in truncating the ftp site for DNA/GUI. It is available from fly.bio.indiana.edu (129.79.225.25) in the directory /molbio/unix as dna-gui.tar.Z. Mark -- Mark E. Berres Birge Hall Office, Room 440 Department of Genetics 430 Lincoln Drive Department of Zoology Madison, WI. 53706-1313 University of Wisconsin at Madison Ph: (608)-265-6427 -----BEGIN PGP PUBLIC KEY BLOCK----- Version: 2.6.2 mQCNAy9eAb0AAAEEALiWUAWSEV4g9Glu0Al8VxA62PBkfeSZJC/90guCvZtwgDvC fLDA862kj9Bs5Ae4KwsbYag6QSkG1JqfHzufAFLgTgkt5k68TLfEAsut/WMy8ix3 wVoQAZTbQnOTeGmG2ceA1cYY9pDuzrUAt7vhpBc9pp4rEHXX2Uljqit9IVslAAUR tC1NYXJrIEUuIEJlcnJlcyA8bWFya2JAcmF2ZWwuem9vbG9neS53aXNjLmVkdT4= =IUu8 -----END PGP PUBLIC KEY BLOCK----- From owner-rapd@net.bio.net Mon Apr 17 23:00:00 1995 Path: biosci!rutgers!gatech!howland.reston.ans.net!agate!darkstar.UCSC.EDU!cats-po-1.ucsc.edu!tschulz From: tschulz@cats.ucsc.edu (Thomas Schulz) Newsgroups: bionet.molbio.rapd Subject: REQ: primers for rapd Date: Tue, 18 Apr 1995 08:22:01 Organization: Institute of Marine Sciences Lines: 14 Message-ID: <2F93D919tschulz@cats.ucsc.edu> NNTP-Posting-Host: tsa-38.ucsc.edu Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" X-Mailer: InternetWorks Mail and News 1.1 I'm looking for a place to buy a set of primers to use for rapd's. I've heard a company called Operon sells a set of 100, but they want $150 for it. I'm hoping there must be some company or group that's making a lot of oligo- nucleotides anyway and could spare some for me at a much more reasonable price. Any suggestions on who to contact would be much appreciated. **************************************************************************************** Thomas R. Schulz tschulz@cats.ucsc.edu A316 EMS Bldg. Institute of Marine Sciences Ph. (408) 425-7242 University of California fax: (408) 459-4882 Santa Cruz, CA 95064 **************************************************************************************** From owner-rapd@net.bio.net Tue Apr 18 23:00:00 1995 Path: biosci!ns1.faseb.org!darwin.sura.net!nntp.usm.edu!whale.st.usm.edu!sywang From: sywang@whale.st.usm.edu (Shiao Y. Wang) Newsgroups: bionet.molbio.rapd Subject: Re: REQ: primers for rapd Date: 19 Apr 1995 04:16:38 GMT Organization: University of Southern Mississippi Lines: 16 Message-ID: <3n22r6$g6v@server.st.usm.edu> References: <2F93D919tschulz@cats.ucsc.edu> NNTP-Posting-Host: whale.st.usm.edu X-Newsreader: TIN [version 1.2 PL1] Thomas Schulz (tschulz@cats.ucsc.edu) wrote: : I'm looking for a place to buy a set of primers to use for rapd's. I've heard : a company called Operon sells a set of 100, but they want $150 for it. I'm : hoping there must be some company or group that's making a lot of oligo- : nucleotides anyway and could spare some for me at a much more reasonable : price. Any suggestions on who to contact would be much appreciated. I just purchased a set of 100 primers from the Nucleic Acid - Protein Service Unit at the Univ. of British Columbia. $235 for 100 primers. Contact them at hobbs@pop.unixg.ubc.ca By the way, I think the price at Operon is $150 for a set of 20 primers. Shiao Wang University of Southern Mississippi From owner-rapd@net.bio.net Tue Apr 18 23:00:00 1995 Path: biosci!BSUVC.BSU.EDU!00bmmylott From: 00bmmylott@BSUVC.BSU.EDU Newsgroups: bionet.molbio.rapd Subject: RAPD APPLICATIONS Date: 19 Apr 1995 09:18:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HPIRJ0PUXA8ZH062@BSUVC.bsu.edu> Reply-To: 00bmmylott@bsuvc.bsu.edu NNTP-Posting-Host: net.bio.net HELLO NETTERS!! I AM WRITING A MANUAL FOR MY UNDERGRADUATE THESIS ENTITILED "RAPD PCR FOR THE NON-MAJOR." I'M PLANNING FOR IT TO BE A KIND OF "IDIOT'S GUIDE" TO PCR. IF YOU HAVE ANY IDEAS OR YOU ARE CURRENTLY DOING RESEARCH AS TO ANY NON - GENETIC / MOLECULAR BIOLOGY APPLICATIONS FOR RAPD PCR - PLEASE SWING THEM MY WAY AND I'LL PUT YOU IN MY ACKNOWLEDGEMENTS!! (I KNOW I'M TOO GIVING, BUT..)tTHANKS FOR THE ASSIST, BRANDT MICHAEL MYLOTT BALL STATE UNIVERSITY MUNCIE, IN From owner-rapd@net.bio.net Wed Apr 19 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!alfa02.medio.net!netnews1.nwnet.net!netnews.nwnet.net!oracle.pnl.gov!bbs.pnl.gov!mf_romine2.pnl.gov!user From: mf_romine@pnl.gov (Margaret Romine) Newsgroups: bionet.molbio.rapd Subject: Re: Phylip program Date: Tue, 18 Apr 1995 07:39:19 -0800 Organization: Pacific Northwest Lab Lines: 11 Message-ID: References: <3m3dmq$q63@tribune.usask.ca> NNTP-Posting-Host: mf_romine2.pnl.gov In article <3m3dmq$q63@tribune.usask.ca>, LEES@skyfox.usask.ca (Lee, Sun ) wrote: > Hello, wonder where I can find Phylip program in FTP site or > introductory document on this? > > Thanks, > > Lee try ftp.bio.indiana.edu From owner-rapd@net.bio.net Thu Apr 20 23:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!pipex!oleane!jussieu.fr!univ-lyon1.fr!swidir.switch.ch!scsing.switch.ch!news.rediris.es!news.uam.es!netadmin From: Joaquin Dopazo Newsgroups: bionet.molbio.rapd Subject: Re: anyone score gels w/ image analysis? Date: 21 Apr 1995 09:39:06 GMT Organization: Universidad Autonoma de Madrid, Spain Lines: 26 Message-ID: <3n7ufq$5ph@ns.uam.es> References: NNTP-Posting-Host: chotis.cnb.uam.es cga3@cornell.edu (Christopher G. Alpha) wrote: > > Hello all! I am looking for information on the possiblity or lack thereof > of scoring RAPDs gels with image analysis software. For example scanning > in a gel photo and then importing it into an analysis program. Any > thoughts or suggestions on this topic would be greatly appreciated, you may > reply here or via Email. > > Thanx again! > > CGA Check a new program recently released by TDI which includes analysis of RAPD data (as well as microsatellite, RFLP and other fingerprinting techniques). This progras has been developped at Centro Nacional de Biotecnologia (Madrid, Spain) under a contract with TDI and we will have in a few days a DEMO available at out FTP server and at our WWW server (by the way, you can have a look at WWW.CNB.UAM.ES). The program runs on PC compatible computers and reads the most common image formats (PCX, TIFF, BMP, etc). We have extensively tested the prograsm and it performs very well even with images adquired using a hand scanner over a not very clean polaroid picture. Unfortunately TDI has not email available yet, but you can ask them for a DEMO at the FAX number: 34-1-4095126. Ximo From owner-rapd@net.bio.net Thu Apr 20 23:00:00 1995 Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.rapd Path: biosci!rutgers!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!news.ksu.ksu.edu!bubba.ucc.okstate.edu!constellation!news.ecn.uoknor.edu!news.uoknor.edu!ns1.nodak.edu!hugo From: e_hugo@dsu1.dsu.nodak.edu (Eric R. Hugo) Subject: PCR in positive displacement micropipettes--info requested Sender: usenet@ns1.nodak.edu (Usenet login) Message-ID: <3n3tbf$da8_001@chem.dsu.NoDak.edu> Date: Wed, 19 Apr 1995 20:55:11 GMT Nntp-Posting-Host: hugo.chem.dsu.nodak.edu Organization: Dickinson State University X-Newsreader: News Xpress Version 1.0 Beta #3 Followup-To: bionet.molbio.methds-reagnts Lines: 16 Xref: biosci bionet.molbio.methds-reagnts:27496 bionet.molbio.rapd:1107 Dear Bionetters, Does anyone have a reference for performing PCR in positive displacement pipettes. We have an MJ Res. minicycler with an optional block for doing PCR in micropipette tips. I have not been able to track down a good protocol or reference to suppliers. Any help will be appreciated. Please email any replies and I will post a summary to save on bandwidth. Thanks in advance. Eric ;-) // \\ // \\ // \\ // \\ // \\ // \\ // \\ Eric Hugo, Ph.D.// |:,\\': | \\ // | :,\\': | \\ // | :,\\': | \\ e_hugo@dsu1.dsu.nodak.edu\ | | \\ // | | // \\ | | \\ // | | // \\ | | Asst. Professor, Biology \\ | :,\\': | // \\ | :,\\': | // \\ | Dickinson State University \\ // \\ // \\ // \\ // \\ Dickinson, ND 58601 |PGP 2.6 Key available from most key servers From owner-rapd@net.bio.net Mon Apr 24 23:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ Date: 25 Apr 1995 02:00:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 347 Sender: daemon@net.bio.net Distribution: world Message-ID: <199504250900.CAA15425@net.bio.net> NNTP-Posting-Host: net.bio.net Four important items follow: How to cancel e-mail subscriptions to BIOSCI newsgroups, BIOSCI archive searching, the BIOSCI FAQ, and the BIOSCI User Address Directory form. If you have not yet listed yourself in our BIOSCI user directory, please take a few minutes to complete and return the form below. 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For example: comment: ARABIDOPSIS PLANT-BIOLOGY BIONEWS On the comment: lines use these names below ---- NOT the USENET names below MAILING LIST NAME USENET Newsgroup Name ----------------- --------------------- ACEDB-SOFT bionet.software.acedb AGEING bionet.molbio.ageing AGROFORESTRY bionet.agroforestry ARABIDOPSIS bionet.genome.arabidopsis ASCB bionet.prof-society.ascb BIOCAN bionet.prof-society.cfbs BIOFORUM bionet.general BIO-INFORMATION-THEORY bionet.info-theory BIONAUTS bionet.users.addresses BIONEWS bionet.announce BIO-JOURNALS bionet.journals.contents BIO-MATRIX bionet.molbio.bio-matrix BIOPHYSICAL-SOCIETY bionet.prof-society.biophysics BIOPHYSICS bionet.biophysics BIO-SOFTWARE bionet.software BIOTHERMOKINETICS bionet.metabolic-reg BIO-WWW bionet.software.www CARDIOVASCULAR-RESEARCH bionet.biology.cardiovascular CELEGANS bionet.celegans CELL-BIOLOGY bionet.cellbiol CHLAMYDOMONAS bionet.chlamydomonas CHROMOSOMES bionet.genome.chromosomes COMPUTATIONAL-BIOLOGY bionet.biology.computational CSM bionet.prof-society.csm CYTONET bionet.cellbiol.cytonet DROSOPHILA bionet.drosophila EMBL-DATABANK bionet.molbio.embldatabank EMF-BIO bionet.emf-bio EMPLOYMENT bionet.jobs EMPLOYMENT-WANTED bionet.jobs.wanted FASEB bionet.prof-society.faseb GDB bionet.molbio.gdb GENBANK-BB bionet.molbio.genbank GENETIC-LINKAGE bionet.molbio.gene-linkage GRASSES-SCIENCE bionet.biology.grasses HIV-MOLECULAR-BIOLOGY bionet.molbio.hiv HUMAN-GENOME-PROGRAM bionet.molbio.genome-program IMMUNOLOGY bionet.immunology INFO-GCG bionet.software.gcg JOURNAL-NOTES bionet.journals.note METHODS-AND-REAGENTS bionet.molbio.methds-reagnts MICROBIOLOGY bionet.microbiology MOLECULAR-EVOLUTION bionet.molbio.evolution MOLECULAR-MODELLING bionet.molec-model MOLLUSC-MOLECULAR-NEWS bionet.molbio.molluscs MYCOLOGY bionet.mycology NEUROSCIENCE bionet.neuroscience N2-FIXATION bionet.biology.n2-fixation PARASITOLOGY bionet.parasitology PHOTOSYNTHESIS bionet.photosynthesis PLANT-BIOLOGY bionet.plants POPULATION-BIOLOGY bionet.population-bio PROTEIN-ANALYSIS bionet.molbio.proteins PROTEIN-CRYSTALLOGRAPHY bionet.xtallography PROTISTA bionet.protista RAPD bionet.molbio.rapd SCIENCE-RESOURCES bionet.sci-resources STADEN bionet.software.staden STRUCTURAL-NMR bionet.structural-nmr TROPICAL-BIOLOGY bionet.biology.tropical URODELES bionet.organisms.urodeles VIROLOGY bionet.virology WOMEN-IN-BIOLOGY bionet.women-in-bio YEAST bionet.molbio.yeast ZBRAFISH bionet.organisms.zebrafish Listing newsgroups on the comment: line is optional, of course. Thanks again for your cooperation! --------------- please cut here and return portion below --------------- New information or Update to old record (enter N or U): date (DD-MM-YY): first name: middle initial: family name: job title: e-mail address: e-mail network: phone number: FAX number: institution: address1: address2: address3: city: state/province: country: postal code: research interest: research interest: comment: comment: comment: comment: comment: From owner-rapd@net.bio.net Mon Apr 24 23:00:00 1995 Path: biosci!acadiau.ca!marlene.snyder From: marlene.snyder@acadiau.ca ("MARLENE SNYDER") Newsgroups: bionet.molbio.rapd Subject: Please subscribe Date: 25 Apr 1995 06:57:57 -0700 Organization: Acadia University Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <14AE0A721D3@ace.acadiau.ca> NNTP-Posting-Host: net.bio.net Hello: My lab here at Acadia University we use RAPDs to examine various population and generation questions using damselflies, honey bees, a small amphipod and blandings turtles. The information passed around in this mailing list will likely be very useful to us. Please subscribe me to the mailing list. Dr. Marlene Snyder From owner-rapd@net.bio.net Thu Apr 27 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: RAPD news letter Newsgroups: bionet.molbio.rapd Subject: postdoc position available Date: 28 Apr 1995 15:20:20 +0100 Lines: 47 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3nqtj4$o87@mserv1.dl.ac.uk> Encoding: 47 TEXT Original-To: RAPD newsgroup POSTDOCTORAL FELLOWSHIP AVAILABLE The Department of Plant Population Biology of the Netherlands Institute of Ecological Research, Center of Terrestrial Ecology (NIOO-CTO), Heteren, The Netherlands, has a post-doc fellowship available for a (Plant) Molecular Biologist The post-doc will work on the project: 'Development of Microsatellite DNA markers in Taraxacum'. A gene library of Taraxacum officinale (common dandelion) will be constructed and screened for short tandem repeats. Selected plasmid inserts will be sequenced and PCR primers of flanking regions will be designed and tested. Stability, level of polymorphism and inheritance of microsatellite markers will be inves- tigated. The duration of the project is 1 year, extendable to 2 years. This project is part of the research programme 'Evolution and Ecology of sexual and asexual Taraxacum officinale', a col- laboration between the universities of Amsterdam, Utrecht and Wageningen and the NIOO-CTO. It is financed by the Netherlands Organization for Scientific Research (NWO). The stipend is around Dfl 3000 per month. Dutch citizens are excluded from the fellowship. The NIOO-CTO has excellent, new facilities for molecular biological research. Molecular markers are used as tools for ecological and evolutionary studies in populations of animals, plants and micro-organisms. For further information contact: Dr. Peter van Dijk (phone 31 8306 91208; fax 31 8306 23227; Email pjvandijk@nioo.nl) Letters should include a curriculum vitae, a list of publications and names of referents (with fax and email ad- dresses), and can be send to: Mr. P. Peters, NIOO-CTO, PO Box 40, 6666 ZG Heteren, The Netherlands, before May 17th 1995. From owner-rapd@net.bio.net Thu Apr 27 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: RAPD news letter Newsgroups: bionet.molbio.rapd Subject: Amplification of IGS of rDNA Date: 28 Apr 1995 11:49:21 +0100 Lines: 31 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3nqh7h$fi3@mserv1.dl.ac.uk> Encoding: 31 TEXT Original-To: RAPD newsgroup Dear all, Boehringer Mannheim say that they have polymerases that can amplify in PCR up to 10 Kb and more. Therefore it may be possible to amplify the Inter Genic Spacer (IGS) of the rDNA genes using conserved primers form the 26 S and the 18S genes and look for restriction enzyme polymorphism. My questions are: 1. Has anyone experience with such thermostable polymerases for long PCR fragments? 2. Has anyone successfully amplified IGS regions (in Taraxacum about 6 Kb)? 3. Which primers have been used? Thanks for replies, Peter Van Dijk Dept. of Plant Population Biology Netherlands Institute of Ecology (NIE-CTE) PO box 40 NL-6666 ZG HETEREN, The Netherlands Email: pjvandijk@nioo.nl From owner-rapd@net.bio.net Thu Apr 27 23:00:00 1995 Path: biosci!ASRR.ARSUSDA.GOV!dkaplan From: dkaplan@ASRR.ARSUSDA.GOV (David Kaplan) Newsgroups: bionet.molbio.rapd Subject: Re: Amplification of IGS of rDNA Date: 28 Apr 1995 06:24:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <3nqh7h$fi3@mserv1.dl.ac.uk> NNTP-Posting-Host: net.bio.net We have amplified shorter IGS and ITS regions in plant parasitic nematodes using Amplitaq and primers designed to C. elegans. We were able to attain full sequence. OUr problem was concern regarding microheterogenity among spacer regions within an individual nematode. Anyone have any experience with that? D. Kaplan USDA-ARS Orlando, FL On 28 Apr 1995, RAPD news letter wrote: > > Dear all, > > Boehringer Mannheim say that they have polymerases that can > amplify in PCR up to 10 Kb and more. Therefore it may be possible to > amplify the Inter Genic Spacer (IGS) of the rDNA genes using > conserved primers form the 26 S and the 18S genes and look for > restriction enzyme polymorphism. > My questions are: > 1. Has anyone experience with such thermostable polymerases > for long PCR fragments? > 2. Has anyone successfully amplified IGS regions (in Taraxacum > about 6 Kb)? > 3. Which primers have been used? > > > Thanks for replies, > > > Peter Van Dijk > > Dept. of Plant Population Biology > Netherlands Institute of Ecology (NIE-CTE) > PO box 40 > NL-6666 ZG HETEREN, The Netherlands > > Email: pjvandijk@nioo.nl > > > > > > From owner-rapd@net.bio.net Thu Apr 27 23:00:00 1995 Path: biosci!CCVAX.MMC.EDU!WILLIA48 From: WILLIA48@CCVAX.MMC.EDU Newsgroups: bionet.molbio.rapd Subject: Re: amplification of IGS of rDNA Date: 28 Apr 1995 09:35:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HPVD1J6I5U8WX1JY@CCVAX.MMC.EDU> NNTP-Posting-Host: net.bio.net In response to David Kaplan's question regarding intraindividual sequence heterogeneity. Yes, it can be found ... for a reference check: Vogler & DeSalle Mol. Biol. Evol. (1994) 11:393. In looking at 50 clones of ITS-1 from tiger beetles they find 42 sequences that are unique by at least one nucleotide difference. Is this important? Depends on what you are trying to do. Scott Williams From owner-rapd@net.bio.net Fri Apr 28 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!news.moneng.mei.com!news.ecn.bgu.edu!newspump.wustl.edu!news.miami.edu!usenet.ufl.edu!usenet From: "A. F. Cockburn" Newsgroups: bionet.molbio.rapd Subject: Re: Amplification of IGS of rDNA Date: 28 Apr 1995 15:42:39 GMT Organization: University of Florida Lines: 45 Message-ID: <3nr2df$s1j@no-names.nerdc.ufl.edu> References: <3nqh7h$fi3@mserv1.dl.ac.uk> NNTP-Posting-Host: ppp-06-nerdc-ts2.nerdc.ufl.edu RAPD news letter wrote: > > > Dear all, > > Boehringer Mannheim say that they have polymerases that can > amplify in PCR up to 10 Kb and more. Therefore it may be possible to > amplify the Inter Genic Spacer (IGS) of the rDNA genes using > conserved primers form the 26 S and the 18S genes and look for > restriction enzyme polymorphism. > My questions are: > 1. Has anyone experience with such thermostable polymerases > for long PCR fragments? > 2. Has anyone successfully amplified IGS regions (in Taraxacum > about 6 Kb)? > 3. Which primers have been used? > > > Thanks for replies, > > > Peter Van Dijk > > Dept. of Plant Population Biology > Netherlands Institute of Ecology (NIE-CTE) > PO box 40 > NL-6666 ZG HETEREN, The Netherlands > > Email: pjvandijk@nioo.nl We have been amplifying IGSs from many species of mosquitoes. It is pretty easy since they are only 2 kb long or so. We designed our own primers based on reported sequences of 18S and 28S from mosquitoes. The 5' end of the 18S is highly conserved, so it was easy to design a primer there. The 3' end of the 28S is not very conserved- we ended up going in almost 1 kb to find something useful. One possible problem is length variability between individual copies. In some species, we do not get a visible band on PCR because the products are spread out over a 1 kb range. Andrew Cockburn USDA From owner-rapd@net.bio.net Sat Apr 29 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!Germany.EU.net!EU.net!uunet!in1.uu.net!news.inhouse.compuserve.com!news.production.compuserve.com!news From: C.Cooper <74274.3110@CompuServe.COM> Newsgroups: bionet.molbio.rapd Subject: DNA -- Child to parent--HELP Date: 30 Apr 1995 18:05:56 GMT Organization: Writer Lines: 21 Message-ID: <3o0ji4$gv4$1@mhade.production.compuserve.com> For a lay article, I need help understanding one aspect of DNA testing. This is a situation in which the DNA of a man and woman, married, no longer living, is needed to be matched to a forensic sample that has been found. The son of the man and woman has offered to make his own blood or other sample available. I understand that mitochondrial DNA can determine the mother's DNA, but I have questions left. My question are 1) Can the son's sample be used successfully to determine both the mother's and the father's DNA and how; Is this how paternity testing is routinely done? 2) If the DNA cannot be accomplished from the son's sample alone, can the son's sample be used in conjunction with a) one parent's [ie the mother's, which may be available] or b) with the brother of the father 3) If these DNA patterns are successfully extracted [is that the right term?], can they be tested for a match against the forensic sample? Any help to answer all or some of these questions, will be most appreciated. C.Cooper -- C.Cooper: My e-mail address is: INTERNET:74274.3110@compuserve.com From owner-rapd@net.bio.net Sun Apr 30 23:00:00 1995 Path: biosci!niai.affrc.go.jp!masashi From: masashi@niai.affrc.go.jp (Masashi Takahashi) Newsgroups: bionet.molbio.rapd Subject: help Date: 1 May 1995 00:01:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199505010658.PAA05936@ss.niai.affrc.go.jp> NNTP-Posting-Host: net.bio.net help