From owner-rapd@net.bio.net Mon May 01 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!vixen.cso.uiuc.edu!newsrelay.iastate.edu!bvc.edu!yuqian From: yuqian@bvc.edu (Suicidal Freshman) Newsgroups: bionet.molbio.proteins,bionet.molbio.rapd Subject: New Dedicated Bio-tech/science/chem BBS Message-ID: <1995May2.123420.10885@bvc.edu> Date: 2 May 95 12:34:20 CDT Organization: Buena Vista College, Storm Lake, IA Lines: 27 Xref: biosci bionet.molbio.proteins:4430 bionet.molbio.rapd:1118 %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% A NEW BBS IS ONLINE AND NEEDS PEOPLE LIKE YOU TO HELP IT PROSPER The IDT Online Information System is now up and running and needs intelligent users such as yourselves. Anyone interested in Chemistry or Biology or any twist of the two is more than welcome. If you are a user of Oligonucleotides, there is an online order system that allows you to place your order directly into the production facility for 48 hour turnaround. THERE IS ABSOLUTELY NO CHARGE FOR USAGE. YOU DO NOT HAVE TO BE A CUSTOMER TO USE THE SYSTEM Just a sample of some of the discussion forums: Parsitology Classifieds Employment Chemical Engineering Computer Software Biochemistry Programming Molecular Medicine Genetics Organic Chemistry Oligo Design Scientific Humor BioPhysics Cell Biology Internet Topics Biomed Engineering Late Breaking News Immunology NeuroScience Suggestions The system is in place, and we are looking for good users, and a few Forum Moderators, so: Check it out: telnet 204.71.106.202 or telnet idtdna.com %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% From owner-rapd@net.bio.net Mon May 01 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!newsrelay.iastate.edu!bvc.edu!yuqian From: yuqian@bvc.edu (Suicidal Freshman) Newsgroups: bionet.molbio.proteins,bionet.molbio.rapd Subject: New Dedicated Bio-tech/science/chem BBS Message-ID: <1995May2.123306.10882@bvc.edu> Date: 2 May 95 12:33:06 CDT Organization: Buena Vista College, Storm Lake, IA Lines: 27 Xref: biosci bionet.molbio.proteins:4429 bionet.molbio.rapd:1117 %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% A NEW BBS IS ONLINE AND NEEDS PEOPLE LIKE YOU TO HELP IT PROSPER The IDT Online Information System is now up and running and needs intelligent users such as yourselves. Anyone interested in Chemistry or Biology or any twist of the two is more than welcome. If you are a user of Oligonucleotides, there is an online order system that allows you to place your order directly into the production facility for 48 hour turnaround. THERE IS ABSOLUTELY NO CHARGE FOR USAGE. YOU DO NOT HAVE TO BE A CUSTOMER TO USE THE SYSTEM Just a sample of some of the discussion forums: Parsitology Classifieds Employment Chemical Engineering Computer Software Biochemistry Programming Molecular Medicine Genetics Organic Chemistry Oligo Design Scientific Humor BioPhysics Cell Biology Internet Topics Biomed Engineering Late Breaking News Immunology NeuroScience Suggestions The system is in place, and we are looking for good users, and a few Forum Moderators, so: Check it out: telnet 204.71.106.202 or telnet idtdna.com %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% From owner-rapd@net.bio.net Tue May 02 23:00:00 1995 Path: biosci!ASRR.ARSUSDA.GOV!abartlet From: abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") Newsgroups: bionet.molbio.rapd Subject: Re: Stoffel Framgent and RAPD Date: 3 May 1995 10:43:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net We have used Stoeffel fragment exclusively for our insect RAPD work and are happy with the results. I think it is somewhat cheaper than TAQ, so if it works for you, then it is probably worth it. Alan C. Bartlett "GENES-R-US" "ALTERATIONS AVAILABLE!^" "^some restrictions may apply:)" From owner-rapd@net.bio.net Tue May 02 23:00:00 1995 Path: biosci!RS6000.CMP.ILSTU.EDU!jadole From: jadole@RS6000.CMP.ILSTU.EDU (Jeff Dole) Newsgroups: bionet.molbio.rapd Subject: Re: Bulk Segregant Analysis Date: 3 May 1995 09:54:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <9505031653.AA76417@rs6000.cmp.ilstu.edu> NNTP-Posting-Host: net.bio.net Adam Marchant asks: >Could someone please give me a reference for a clear exposition of the >principles of bulk segregant analysis using RAPDs - the theory, as well as >the practical details? Here's the original reference: Michelmore, R. W., I. Paran and R. V. Kesseli, 1991 Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the National Academy of Science 88: 9828 - 9832. There are many more recent references, but this should help. Jeff Dole Biology Department Illinois State University Normal, IL 61701 (309) 438-7351 jadole@ilstu.edu > > > > > From owner-rapd@net.bio.net Tue May 02 23:00:00 1995 Path: biosci!cabi.org!D.BRAYFORD From: D.BRAYFORD@cabi.org ("David Brayford ", IMI) Newsgroups: bionet.molbio.rapd Subject: SSRSSRSSRSSR Date: 3 May 1995 04:08:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <2FA7642A@msm.cgnet.com> NNTP-Posting-Host: net.bio.net Hi All I've been trying SSR-primer PCR with some fungi and have got nice fingerprint-like banding patterns with e.g. (CAG)x5 and (TCC)x5, but with (CAT)x5 all I get is a smear at the top of the gel. My interpretation of this is that: (a). amplification is happening, so presumably target sequences are present; but (b). these are not dispersed, just one long lump. Has anybody else found similar things? Do you agree with my interpretation? Maybe there's something more complicated going on? Cheers, Dave Brayford d.brayford@cabi.org From owner-rapd@net.bio.net Tue May 02 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!cs.utexas.edu!not-for-mail From: marchaa@agric.nsw.gov.au (A.Marchant) Newsgroups: bionet.molbio.rapd Subject: Bulk Segregant Analysis Date: 2 May 1995 23:38:47 -0500 Organization: UTexas Mail-to-News Gateway Lines: 18 Sender: nobody@cs.utexas.edu Message-ID: NNTP-Posting-Host: news.cs.utexas.edu Could someone please give me a reference for a clear exposition of the principles of bulk segregant analysis using RAPDs - the theory, as well as the practical details? One thing that I am interested in is the possibility of finding markers, using bulk segregant analysis, when near-inbred lines are not available. Thanks very much, Adam Marchant Agricultural Research Institute Wagga Wagga, NSW Australia marchaa@agric.nsw.gov.au From owner-rapd@net.bio.net Tue May 02 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!pipex!warwick!griffin.nott.ac.uk!pmbcjz.nottingham.ac.uk!Chris_Jones From: Chris_Jones@VME.CCC.NOTTINGHAM.AC.UK (Chris Jones) Newsgroups: bionet.molbio.rapd Subject: Stoffel Framgent and RAPD Date: Wed, 3 May 1995 08:31:58 Organization: Univ. of Nottingham Biochemistry Dept Lines: 8 Message-ID: NNTP-Posting-Host: pmbcjz.nottingham.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Hello ,I went to an ABI seminar yesterday and they claimed that Stoffel fragment of Taq was better for RAPD. I have also dug up a paper from LaJolla where this is claimed. Has anyone experience of getting better RAPD results from Stoffel. Is there extra cost and are the results woth it? Thanks Chris Jones From owner-rapd@net.bio.net Tue May 02 23:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.molbio.rapd Subject: Re: Bulk Segregant Analysis Date: 3 May 1995 05:47:08 -0700 Organization: University of Arkansas Lines: 44 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net > To: rapd@net.bio.net > From: marchaa@agric.nsw.gov.au (A.Marchant) > Subject: Bulk Segregant Analysis > Date: 2 May 1995 23:38:47 -0500 > Could someone please give me a reference for a clear exposition of the > principles of bulk segregant analysis using RAPDs - the theory, as well as > the practical details? > > One thing that I am interested in is the possibility of finding > markers, using bulk segregant analysis, when near-inbred lines are not > available. > > Thanks very much, > > Adam Marchant > Agricultural Research Institute > Wagga Wagga, NSW > Australia > > marchaa@agric.nsw.gov.au The original reference for Bulked Segregant Analysis (BSA) is from Michelmore,RW; Paran,I; Kesseli,RV (1991): Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. PNAS, USA 88, 9828-9832. A good article on the use of BSA (as well as other approaches is Tanksley,SD; Ganal,MW; Martin,GB (1995): Chromosome landing: a paradigm for map-based gene cloning in plants with large genomes. T.I.G. 11, 63- 68. By the way, I believe NIL stands for Near-Isogenic Lines. //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// From owner-rapd@net.bio.net Tue May 02 23:00:00 1995 Path: biosci!BRADLEY.BRADLEY.EDU!rrs From: rrs@BRADLEY.BRADLEY.EDU (Robert Stephens) Newsgroups: bionet.molbio.rapd Subject: Re: Bulk Segregant Analysis Date: 3 May 1995 14:46:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net And T. I. G. stands for---- Thanks rrs@bradley.bradley.edu Robert Rhea Stephens Biology Department Bradley University Peoria, IL 61625 From owner-rapd@net.bio.net Tue May 02 23:00:00 1995 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!pipex!warwick!news.ncl.ac.uk!usenet From: Guy Reeves Newsgroups: bionet.molbio.rapd Subject: sizing DNA using RAPDS? Date: 28 Apr 1995 13:24:16 GMT Organization: Newcastle University Lines: 23 Message-ID: <3nqqa0$o0s@whitbeck.ncl.ac.uk> NNTP-Posting-Host: beck16.ncl.ac.uk X-Newsreader: WinVN 0.92.5 I am interested in finding out the maximum amplifyable length of DNA from samples that have been either degraded and/or chemically modified. I am thinking of trying RAPDs to do this, using the assumption that the largest fragment amplified will be a reasonable indicator of the size of DNA that could be amplified using conventional PCR primers. I have no experience of using RAPDs and would be grateful for any body's opinion as to whether it is a feasible approach or if anybody has successfully applied such an approach? I am thinking of using several RAPD primers in the same reaction or useing very short (6mers) primers to ensure that lack of suitably complementary sites doesn't limit the approach. Thanks in advance for any help. Guy From owner-rapd@net.bio.net Wed May 03 23:00:00 1995 Path: biosci!HARPO.TNSTATE.EDU!GAWELN From: GAWELN@HARPO.TNSTATE.EDU Newsgroups: bionet.molbio.rapd Subject: Stoffel fragment and RAPDs Date: 4 May 1995 06:05:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HQ3J9B3M028WXFHY@HARPO.TNSTATE.EDU> NNTP-Posting-Host: net.bio.net I have used Stoffel fragment to generate RAPDs from a number of genera and have had good results. However, a plant I have worked with recently produced only very small fuzzy bands with Stoffel, but clear sharp bands with standard Taq. So you may see some specificity for your DNA. As far as cost goes, I normally use 1.0-1.5 units/reaction. At this concentration Stoffel has been the cheapest of any Taqs I have seen. Nick Gawel Tennessee State University From owner-rapd@net.bio.net Wed May 03 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!news2.near.net!news3.near.net!saturn.caps.maine.edu!maine.maine.edu!io20109 Organization: University of Maine System Date: Wed, 3 May 1995 22:30:23 EDT From: Message-ID: <95123.223023IO20109@MAINE.MAINE.EDU> Newsgroups: bionet.molbio.rapd Subject: Re: Stoffel Framgent and RAPD References: Lines: 6 I use Stoffel fragment with DNA from seaweed (Fucus) and it yields more bands, especially of smaller sizes. I am quite happy with it. It also seems to be less sensitive to small variations in MgCl2, and that helps to make results consistent, which is very important when doing RAPD. Ester Serrao From owner-rapd@net.bio.net Wed May 03 23:00:00 1995 Path: biosci!acadiau.ca!marlene.snyder From: marlene.snyder@acadiau.ca ("MARLENE SNYDER") Newsgroups: bionet.molbio.rapd Subject: NaOH in primers Date: 4 May 1995 10:06:34 -0700 Organization: Acadia University Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <2260A4632F6@ace.acadiau.ca> NNTP-Posting-Host: net.bio.net Andre Hamel suggested using NaOH at 10 mM in RAPD primer solutions in order to prevent aggregation. We have tried using the NaOH but are still having mixed results. I am wondering if others are using NaOH in their primers, and what the consensus is on whether it is indeed a good idea to store primers in NaOH. From owner-rapd@net.bio.net Sat May 06 23:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.molbio.rapd Subject: Re: Bulk Segregant Analysis Date: 7 May 1995 09:25:17 -0700 Organization: University of Arkansas Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <387A547BA@mercury.uark.edu> NNTP-Posting-Host: net.bio.net > Date: Wed, 3 May 1995 16:45:06 -0500 (CDT) > From: Robert Stephens > Subject: Re: Bulk Segregant Analysis > To: Douglas Rhoads > Cc: rapd@net.bio.net > And T. I. G. stands for---- > Thanks > > rrs@bradley.bradley.edu > Robert Rhea Stephens > Biology Department > Bradley University > Peoria, IL 61625 > > Trends in Genetics. //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// From owner-rapd@net.bio.net Sat May 06 23:00:00 1995 Path: biosci!CGNET.COM!N.HUANG From: N.HUANG@CGNET.COM Newsgroups: bionet.molbio.rapd Subject: classification with STS Date: 6 May 1995 23:19:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HQ83G0ZWMO004JNM@irri.cgnet.com> NNTP-Posting-Host: net.bio.net Dear Netters, We like to classify rice germplasm with STS plus restriction digestion. This might be better than RAPD as the STS is highly reproducible. We however have problem on how to score the STS bands after restriction digestion. We commonly obtained a few to several dozen bands when the PCR products digested with several 4bp cutters. Should we score all bands or score only the polymorphic ones? In both cases we worry about that we would repeatly score the same things again and again as the polymorphism (DNA variation between varieties) can be present in many STS/enzyme combination. Any of you have any idea or know any work (reference) of this kind of work? We need your help. Thanks. Ning Huang IRRI E-mail N.HUANG@CGNET.COM FAX 63-2-891-1292 From owner-rapd@net.bio.net Sat May 06 23:00:00 1995 Newsgroups: bionet.molbio.rapd Path: biosci!agate!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!uunet!in1.uu.net!news.gun.de!news.hamburg.pop.de!nordwest.pop.de!informatik.uni-bremen.de!cs.tu-berlin.de!fu-berlin.de!news.belwue.de!news.belwue.de!scsing.switch.ch!rzusuntk.unizh.ch!botcannabis.unizh.ch!user From: frey@umnw.ethz.ch (Daniel Frey) Subject: gel analysis software Message-ID: Sender: newsadm@rzu-news.unizh.ch (CNEWS ADMINISTRATION) Organization: Geobotanical Institute ETH X-Newsreader: Value-Added NewsWatcher 2.0b24.0+ Date: Wed, 3 May 1995 16:35:33 GMT Lines: 14 Hello folks I would like to know what kind of software do you use for your gel analysis? I tried out GelReader but was not satisfied. I have seen another package running on a DOS machine and it seems to be quite good. I'd really appriciate to get some ideas on prices, features, problems. Thanks in advance Dani :-) ---------------------------------------------------------- Daniel Frey Tel: +41 1 6323854 Geobotanical Institute Fax: +41 1 3854204 Swiss Federal Institute of Technology Zollikerstrasse 107 CH-8008 Zuerich Email frey@umnw.ethz.ch ---------------------------------------------------------- From owner-rapd@net.bio.net Sat May 06 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!uunet!news.inhouse.compuserve.com!news.production.compuserve.com!news From: Cynthia Helphingstine <76657.42@CompuServe.COM> Newsgroups: bionet.molbio.molluscs,bionet.molbio.rapd,bionet.molbio.yeast,sci.bio,sci.bio.technology Subject: Customers of Core DNA Sequencing La Date: 7 May 1995 17:41:54 GMT Organization: The Biotron Group Lines: 11 Distribution: inet Message-ID: <3oj0p2$f60$2@mhadg.production.compuserve.com> Xref: biosci bionet.molbio.molluscs:88 bionet.molbio.rapd:1133 bionet.molbio.yeast:2888 sci.bio:16345 sci.bio.technology:2995 If you manage a lab in the USA that sends DNA samples to a core lab for sequencing, we would like to have you participate in a market research study we are doing. By answering our questions, you will be contributing to the availability of better sequencing products and equipment for all of us. If you would like to participate in this study, please send you name, phone number and the best time of day to reach you to 76657.42@compuserve.com. Our questions will take about 10 minutes. Your responses will be viewed in aggregate only and we will not release your phone number. If you prefer, you can call us at 1-800-592-9575 x 175 after 2:00 am Monday May 8. Thank you! From owner-rapd@net.bio.net Sun May 07 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!swrinde!howland.reston.ans.net!news.cac.psu.edu!news.tc.cornell.edu!travelers.mail.cornell.edu!newsstand.cit.cornell.edu!NewsWatcher!user From: wfl1@cornell.edu (Warren Frank Lamboy) Newsgroups: bionet.molbio.rapd Subject: Tm values, annealing and amplification Followup-To: bionet.molbio.rapd Date: Sun, 07 May 1995 16:34:04 -1200 Organization: Cornell University Lines: 17 Sender: wfl1@cornell.edu (Verified) Message-ID: NNTP-Posting-Host: 132 Can someone please tell me how one utilizes the Tm (melting temperature) of a DNA primer in computing the annealing and amplification temperatures for a PCR amplification? Are there any references in the literature on this? Rummaging around in the dim recesses of my memory, it seems that I once could have answered this question myself, but not any longer. Also, I use the DNASTAR program, and that program gives two different temperatures, the Davis, Botstein, Roth and the Wallace temperatures. Are one or both of these Tm's? Or are they different animals entirely? [I do not have a DNASTAR manual, which I assume would explain this.] Thanks a lot for your help on these questions. -- Warren F. Lamboy "It's easy if you know how to do it, but it's impossible if you don't know how to do it." From owner-rapd@net.bio.net Mon May 08 23:00:00 1995 Path: biosci!rutgers!csn!silo.nrcs.usda.gov!ftcnews.nrcs.usda.gov!usenet From: roberts@tfrl.ars.usda.gov Newsgroups: bionet.molbio.rapd Subject: Re: Gel Analysis Date: 9 May 1995 20:30:05 GMT Organization: USDA-SCS NHQ in Fort Collins Lines: 20 Message-ID: <3oojcd$ong@ftcnews.nrcs.usda.gov> References: Reply-To: roberts@tfrl.ars.usda.gov NNTP-Posting-Host: 162.79.48.2 X-Newsreader: Firefox WinVN 1.0 beta 5 Daniel: I am using a Windows program called GelCompare which, although somewhat difficult to learn because the documentation is somewhat cryptic, is a program with many desirable features, especially for RAPD analyses. One nice feature is the ability to combine gels from different primers into a composite gel for analysis, making primer selection less subjective. Gel Compare is a product of Applied Maths in Kortrijk, Belgium (I have no commercial interest in this company, by the way) Rodney Roberts In article , frey@umnw.ethz.ch says... > >Hello folks > I would like to know what kind of software do you use for your gel >analysis? I use RAPDs on agarose gel and would like to get an analyse of its bands by computer.... From owner-rapd@net.bio.net Mon May 08 23:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.molbio.rapd Subject: Re: (none) Date: 9 May 1995 11:13:25 -0700 Organization: University of Arkansas Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: <3556DA080E@mercury.uark.edu> NNTP-Posting-Host: net.bio.net > To: rapd@net.bio.net > From: Christian.Mouton@greb.ulaval.ca (Christian Mouton) > Subject: (none) > Date: 9 May 1995 09:53:55 -0700 > On May 8 1995, Daniel Frey wrote: > > > Hello folks > > I would like to know what kind of software do you use for your gel > > analysis? I use RAPDs on agarose gel and would like to get an analyse of > > its bands by computer. I tried out GelReader but was not satisfied. I > > have seen > > another package running on a DOS machine and it seems to be quite good. > > I'd really appriciate to get some ideas on prices, features, problems. > > > > Check with P.A.D. Grimont, Institut Pasteur, Paris, who wrote "Taxolab > softwares" for Mac: RestrictoScan, RestrictoTyper, Adanson. They are > intended for ribotyping or RFLP but using an inverted image they perform > extremely well with RAPD fingerprints and are at affordable price. > > pgrimont@pasteur.fr (Patrick A. D. Grimont) > > The postal address is : Institut Pasteur, Service des Entirobactiries, 28 > rue du Docteur-Roux, 75724 Paris cedex 15 France > > regards, > Could you define `affordable' please. To some $5000 US is affordable. But to some of us $50 is affordable. //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// From owner-rapd@net.bio.net Mon May 08 23:00:00 1995 Path: biosci!ASRR.ARSUSDA.GOV!abartlet From: abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") Newsgroups: bionet.molbio.rapd Subject: Re: Gel Analysis Date: 9 May 1995 07:53:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net You could take a look at RFLPscan from Scanalytics, 40 Linnell Circle,=20 Billerica, MA 01821. This programs runs in Windows. It will read TIF or= =20 BMP files from a scanner or other type of system that can produce a=20 graphics file. You can mark lanes and bands, do desmiling and background= =20 corrections, etc. It will accept labels for each lane and molecular=20 weight or rf standards for band analysis and will print and store the=20 results of the analysis. The latest version contains a database function= =20 for comparison of lanes even across gels. The program is kind of=20 expensive, I think it is around $5000 for one user. The 800 number for=20 customer support is 8=FC0-325-3110. I assume they could give you further= =20 information. We have used the program for almost 3 years and have found= =20 it very useful. For example, in the database you can ask questions such=20 as: "Which lanes are the most similar to lane 7" Or "which lanes have=20 bands with a molecular weight (bp) between 430 and 450" etc. The program= =20 has run well on machines from 386 to pentium. Alan C. Bartlett "GENES-R-US" "ALTERATIONS AVAILABLE!^" "^some restrictions may apply:)" From owner-rapd@net.bio.net Mon May 08 23:00:00 1995 Newsgroups: bionet.molbio.rapd Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!Germany.EU.net!news.dfn.de!scsing.switch.ch!rzusuntk.unizh.ch!botcannabis.unizh.ch!user From: frey@umnw.ethz.ch (Daniel Frey) Subject: Gel Analysis Message-ID: Sender: newsadm@rzu-news.unizh.ch (CNEWS ADMINISTRATION) Organization: Geobotanical Institute ETH X-Newsreader: Value-Added NewsWatcher 2.0b24.0+ Date: Mon, 8 May 1995 08:29:21 GMT Lines: 16 Hello folks I would like to know what kind of software do you use for your gel analysis? I use RAPDs on agarose gel and would like to get an analyse of its bands by computer. I tried out GelReader but was not satisfied. I have seen another package running on a DOS machine and it seems to be quite good. I'd really appriciate to get some ideas on prices, features, problems. Thanks in advance Dani :-) ---------------------------------------------------------- Daniel Frey Tel: +41 1 6323854 Geobotanical Institute Fax: +41 1 3854204 Swiss Federal Institute of Technology Zollikerstrasse 107 CH-8008 Zuerich Email frey@umnw.ethz.ch ---------------------------------------------------------- From owner-rapd@net.bio.net Mon May 08 23:00:00 1995 Path: biosci!greb.ulaval.ca!Christian.Mouton From: Christian.Mouton@greb.ulaval.ca (Christian Mouton) Newsgroups: bionet.molbio.rapd Subject: (none) Date: 9 May 1995 09:53:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: <9505091654.AA21845@hermes.ulaval.ca> NNTP-Posting-Host: net.bio.net On May 8 1995, Daniel Frey wrote: > Hello folks > I would like to know what kind of software do you use for your gel > analysis? I use RAPDs on agarose gel and would like to get an analyse of > its bands by computer. I tried out GelReader but was not satisfied. I > have seen > another package running on a DOS machine and it seems to be quite good. > I'd really appriciate to get some ideas on prices, features, problems. > Check with P.A.D. Grimont, Institut Pasteur, Paris, who wrote "Taxolab softwares" for Mac: RestrictoScan, RestrictoTyper, Adanson. They are intended for ribotyping or RFLP but using an inverted image they perform extremely well with RAPD fingerprints and are at affordable price. pgrimont@pasteur.fr (Patrick A. D. Grimont) The postal address is : Institut Pasteur, Service des Entérobactéries, 28 rue du Docteur-Roux, 75724 Paris cedex 15 France regards, -----------------------------Christian Mouton----------------------------- Groupe de Recherche en Écologie Buccale tél. (418)656-5872 Faculté de médecine dentaire fax. (418)656-2861 Université Laval Email: Christian.Mouton@greb.ulaval.ca Québec (Québec) G1K 7P4 CANADA From owner-rapd@net.bio.net Mon May 08 23:00:00 1995 Path: biosci!SERVIDOR.DGSCA.UNAM.MX!carvalho From: carvalho@SERVIDOR.DGSCA.UNAM.MX (Carvalho Torres Alexandro cassio-UACPYP) Newsgroups: bionet.molbio.rapd Subject: Re: Gel Analysis Date: 9 May 1995 08:12:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net On Mon, 8 May 1995, Daniel Frey wrote: > Hello folks > I would like to know what kind of software do you use for your gel > analysis? I use RAPDs on agarose gel and would like to get an analyse of > its bands by computer. I tried out GelReader but was not satisfied. I > have seen > another package running on a DOS machine and it seems to be quite good. > I'd really appriciate to get some ideas on prices, features, problems. > > Thanks in advance Dani :-) > ---------------------------------------------------------- > Daniel Frey Tel: +41 1 6323854 > Geobotanical Institute Fax: +41 1 3854204 > Swiss Federal Institute of Technology > Zollikerstrasse 107 > CH-8008 Zuerich Email frey@umnw.ethz.ch > ---------------------------------------------------------- > > Dear Dani. I use for analysis of my gel The Gel Compar 3.1 for windows. I scanned my negative for the pollaroid photos and after tahta I analyse the tif archive in the program. If youy like I could send you the address of this company. best regards. Alexandro carvalho. FAX 525 513 0010 From owner-rapd@net.bio.net Tue May 09 23:00:00 1995 Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!news.cac.psu.edu!usenet From: mmg6@psu.edu (mmg6) Newsgroups: bionet.molbio.rapd Subject: Re: Tm values, annealing and amplification Date: 10 May 1995 01:48:57 GMT Organization: Penn State University Lines: 6 Sender: mmg6@hearst.cac.psu.edu Message-ID: <3op629$ial@hearst.cac.psu.edu> References: NNTP-Posting-Host: ppp112.cac.psu.edu X-Authinfo-User: mmg6@psu.edu X-Posted-From: InterNews 1.0.4@ppp112.cac.psu.edu X-Authenticated: mmg6 on INN host hearst.cac.psu.edu For oligos 20 bp or less the Tm can be calculated thusly - 2 C for each A or T and 4 C for each G or C - the sum equal the Tm. Cheers, Michael From owner-rapd@net.bio.net Wed May 10 23:00:00 1995 Newsgroups: bionet.molbio.rapd Path: biosci!adam.cc.sunysb.edu!news.sprintlink.net!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!newsspool.doit.wisc.edu!decwrl!tribune.usask.ca!canopus.cc.umanitoba.ca!newsflash.concordia.ca!CC.UMontreal.CA!landryp From: landryp@ERE.UMontreal.CA (Landry Pierre-Alexandre) Subject: number of markers needed in genetic distance ???? Message-ID: Keywords: population, genetic distance Sender: news@cc.umontreal.ca (Administration de Cnews) Organization: Universite de Montreal Date: Thu, 11 May 1995 22:03:47 GMT Lines: 11 I would like to know if anyone ever worked on a relation between the number of RAPD markers and genetic distances calculated? In other words, how many markers should be used to calculate the genetic distance in order to obtain something that will be constant for all the individuals in the same population vs another population? And is this number of markers the same for comparisons of genetic distances between species, families or classes? Thanks in advance, PA From owner-rapd@net.bio.net Wed May 10 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!news.starnet.net!wupost!howland.reston.ans.net!pipex!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: Michal Pater Newsgroups: bionet.molbio.rapd Subject: Dreissena Date: 11 May 1995 09:02:43 +0100 Lines: 14 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3osgb3$bab@mserv1.dl.ac.uk> Original-To: rapd@dl.ac.uk Hello, I would like to investigate Dreissena polymorpha by rapd method. Does anybody work on this material? I am interested in DNA isolation protocol, thermocycler's profile and articles about molecular researches on Dreissena or similer objects. I will be very glad for each information. Michal Pater Department of Genetics US Lukasinskiego 43 71-215 Szczecin Poland katgenmi@uoo.univ.szczecin.pl From owner-rapd@net.bio.net Thu May 11 23:00:00 1995 Path: biosci!SASK.USASK.CA!MARILLIA From: MARILLIA@SASK.USASK.CA Newsgroups: bionet.molbio.rapd Subject: (none) Date: 12 May 1995 07:50:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HQERG5FYRO8WW9ZM@SKYCAT.USask.CA> NNTP-Posting-Host: net.bio.net please unsubscribe me from RAPD From owner-rapd@net.bio.net Sat May 13 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!xlink.net!rz.uni-karlsruhe.de!news.uni-ulm.de!news From: Flavio Ortigao Newsgroups: bionet.molbio.rapd Subject: INTERACTIVA The VIRTUAL LABORATORY Date: 14 May 1995 10:18:04 GMT Organization: University of Ulm, Germany Lines: 15 Message-ID: <3p4lcs$ss4@waage.rz.uni-ulm.de> NNTP-Posting-Host: pintado.physik.uni-ulm.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; IRIX 5.3 IP22) X-URL: news:bionet.molbio.rapd INTERACTIVA was established to design, manufacture and market new products for the biomedical community. We are proud to present the VIRTUAL LABORATORY. Our goal is to provide scientists from all over the world with the virtual access to a real state of the art, laboratory for oligonucleotide and oligopeptide synthesis. We offer more than products, we offer information systems. Welcome to visit us at http://pintado.physik.uni-ulm.de Best regards, Flavio Ramalho Ortigao, CEO -- OLIGONUCLEOTIDES&OLIGOPEPTIDESBIOPOLYMERSWORLDWIDEINFORMATIONSYSTEM J. F. Ramalho Ortigao, PhD, CEO/INTERACTIVA/SEDANSTRASSE 10/D-89077 ULM/ PHONE +49 731 935 79 59/FAX +49 731 935 79 79/E-MAIL flavio.ortigao@physik.uni-ulm.de/URL http://pintado.physik.uni-ulm.de http://www.interactiva.de From owner-rapd@net.bio.net Sun May 14 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!news.uoregon.edu!gaia.ucs.orst.edu!transgene.FSL.ORST.EDU!rottmann From: rottmann@fsl.orst.edu (William H. Rottmann) Newsgroups: bionet.molbio.rapd Subject: quality of primers, UBC vs Operon Date: Mon, 15 May 1995 14:01:02 Organization: Forestry Sciences Lab Lines: 12 Message-ID: NNTP-Posting-Host: transgene.fsl.orst.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Our lab has noticed that more people are using Operon primers as compared to UBC primers. Because we are concerned about the reproducibility of our reactions, we were wondering if there was a technical reason for the apparent preference. Is anyone aware of quality differences between primers from the two sources that affect reproducibility, strength of bands, total yield, background smearing? Will Rottmann Forest Science Oregn State University rottmann@fsl.orst.edu From owner-rapd@net.bio.net Sun May 14 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!demon!doc.news.pipex.net!pipex!uknet!daresbury!not-for-mail From: BANANA & PLANTAIN Newsgroups: bionet.molbio.rapd Subject: Help required on finding e-mail address in Turkey... Date: 15 May 1995 10:58:34 +0100 Lines: 27 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3p78ka$b47@mserv1.dl.ac.uk> Original-To: Plant Biology , RAPD Hello Netters, I would be greatful if anyone can forward an e-mail address for any one of the following from TUBITAK, Marmara Research Center, Department of Biology, Gebze-Kocaeli, Istanbul, Turkey: Gozukirmizi Nermin, Ari S., Gurel F., Gumusel F. or Girakoglu B. Thanks in advance. -Naresh ------------------------------------------------------------------------- NARESH PANCHOLI AGRICULTURAL BOTANY PHONE: +44 1734 875123 EXT. 4087 SCHOOL OF PLANT SCIENCES FAX: +44 1734 316577 UNIVERSITY OF READING READING RG6 6AS ENGLAND N.C.PANCHOLI@READING.AC.UK ------------------------------------------------------------------------- From owner-rapd@net.bio.net Sun May 14 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!uwm.edu!msunews!harbinger.cc.monash.edu.au!news.uwa.edu.au!newsman.csu.murdoch.edu.au!newsman.csu.murdoch.edu.au!usenet From: morgan@numbat.murdoch.edu.au (Una Morgan) Newsgroups: bionet.molbio.rapd Subject: RAP-RNA fingerprinting problems Date: 15 May 1995 01:48:52 GMT Organization: Murdoch University Lines: 25 Sender: -Not-Authenticated-[4945] Message-ID: <3p6bu4INNn0i@newsman.murdoch.edu.au> NNTP-Posting-Host: vetmac2.murdoch.edu.au X-Posted-From: InterNews 1.0.1@vetmac2.murdoch.edu.au Xdisclaimer: No attempt was made to authenticate the sender's name. Help? I've been trying to generate RNA fingerprints using arbitrarily primed PCR on viral RNA. I used Promega AMV RT for the first strand synthesis using the supplied first strand recation buffer and Tth plus polymerase for the second strand reaction. The first couple of reactions I ran looked very promising i.e some background smearing but also numerous distinct bands 1.5kb to 100bp in the test reaction and nothing in the negative controls. (The negative controls consisted of an aliquot of the first strand reaction minus RNA added to the PCR reaction). The last few reactions I have run have resulted in smears in the tests and lots of bands in the negative controls. Increasing the stringency of the second strand reaction results in the disappearence of everything in the tests as well as the negative controls. In theory, DNA contamination should not cause this because AP PCR fingerprinting of DNA requires at least two low stringency cycles to permit initial priming events to occur in opposite directions. Primers that do not normally result in bands in the negative control under standard RAPD condition for DNA amplification are generating numerous bands under these conditions. The reaction temperature for the first strand reaction was 42 degrees and the cycling conditions for the second reaction was one cycle of 94-5 min; 50-5 min; 72-5 min followed by 42 cycles of 94-1 min; 60-1 min and 72-2 min and a final cycle of 72-10 min. Does anyone have any ideas of what I can try next? Thanks Una Morgan From owner-rapd@net.bio.net Sun May 14 23:00:00 1995 Path: biosci!KORYU.STATCI.GO.JP!tsu01135 From: tsu01135@KORYU.STATCI.GO.JP ("R.F.Whittier") Newsgroups: bionet.molbio.rapd Subject: The Accordian Effect Date: 14 May 1995 18:34:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 56 Sender: daemon@net.bio.net Distribution: world Message-ID: <9505150135.AA01658@koryu.statci.go.jp> NNTP-Posting-Host: net.bio.net >Subject: number of markers needed in genetic dist >Date: Thu, 11 May 1995 22:03:47 GMT >From: landryp@ERE.UMontreal.CA (Landry Pierre-Alexandre) >Errors-To: BIOSCI-REQUEST@net.bio.net >To: rapd@net.bio.net >I would like to know if anyone ever worked on a relation >between the number of RAPD markers and genetic distances >calculated? In other words, how many markers should be used to >calculate the genetic distance in order to obtain something >that will be constant for all the individuals in the same >population vs another population? And is this number of >markers the same for comparisons of genetic distances between >species, families or classes? >Thanks in advance, >PA It's a trivial point, but nevertheless of over-riding practical importance that marker mis-scoring expands genetic maps. The more markers used, the greater this accordian effect will be. For each per cent mis-scoring of each marker, the calculated map will expand by as much as 2 bogus centimorgans (one cM on each side of the marker). One mild example of this accordian effect is the map of Arabidopsis thaliana, as based upon mapping of various molecular markers in the recombinant inbred (RI) lines of Lister & Dean (1993, Plant J., 4, 745-750). The original map, based upon 67 loci (66 RFLP and one phenotypic, largest gap: 27.2 cM) indicated a total of 283 cM. Their most recent map sent to the Arabidopsis news group, now with 301 markers, indicates 578 cM separating what were the most distal markers on the original map. This mapping is based upon the same population of RI lines. Actually, the absence of an even greater accordian effect indicates to me that most of the loci were scored with remarkably high accuracy. All the same, I'd lay odds that most of the observed expansion is bogus. RAPD scoring tends to be less accurate than for the sorts of markers used in the Arabidopsis map, although reproducibility varies from marker to marker. In the best RAPD studies I have seen (e.g. Grattapaglia and Sederoff, 1994, Genetics 137: 1121-- 1137) a subset of the most reliable RAPD markers, 5 to 20 cM apart, is selected and used as "framework" markers to establish the overall genetic map, including map distance from end to end. Other markers are then intercalated into this map, without affecting the calculations of map distance between the framework markers. Cheers, Bob Whittier Mitsui Plant Biotechnology Research Institute E-mail: tsu01135@koryu.statci.go.jp From owner-rapd@net.bio.net Mon May 15 23:00:00 1995 Path: biosci!UCRAC1.UCR.EDU!judelson From: judelson@UCRAC1.UCR.EDU (Howard S. Judelson) Newsgroups: bionet.molbio.rapd Subject: Re: quality of primers, UBC vs operon vs. genosys Date: 16 May 1995 12:29:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <199505161925.AA02379@postman> NNTP-Posting-Host: net.bio.net >Our lab has noticed that more people are using Operon primers as compared to >UBC primers. Because we are concerned about the reproducibility of our >reactions, we were wondering if there was a technical reason for the apparent >preference. Is anyone aware of quality differences between primers from the >two sources that affect reproducibility, strength of bands, total yield, >background smearing? I have used all of the Operon primers (1000?), about 300 of the UBC primers, and 20 primers from Genosys. We have been amplifying DNA from Phytophthora infestans. For the UBC and Genosys primers, we had to use about 3 times more primer (by OD based on the manufacturers' data) in order to get decent banding patterns. The bands were usually very faint or nonexistent if we used the standard amounts. About 90% of the Operon primers gave good banding patterns while 70% of the primers from the other sources gave good patterns (using 3X of our standard volume). When the primers worked there was no obvious differences in the number of bands observed. For the UBC primers we frequently ran out of certain primers since we had to use a lot more primer per reaction, and had to get them custom synthesized. Howard Judelson Department of Plant Pathology University of California Riverside, CA 92521 USA From owner-rapd@net.bio.net Mon May 15 23:00:00 1995 Newsgroups: bionet.molbio.rapd Path: biosci!bloom-beacon.mit.edu!gatech!howland.reston.ans.net!vixen.cso.uiuc.edu!uwm.edu!msunews!netnews.upenn.edu!newshost!jtudor From: jtudor@sju.edu (John Tudor) Subject: RAPD Analysis? Message-ID: Organization: Saint Joseph's University X-Newsreader: TIN [version 1.2 PL2] Date: Tue, 16 May 1995 11:52:43 GMT Lines: 6 I have RAPD data for fourteen different strains of bacteria. I would like to arrange them on a relatedness diagram, such as a dendrogram. Does anyone know of a rather simple computer program that I could use that would help me construct such a diagram? If so, is it available at an ftp or www site? Thanks for whatever info you can give. From owner-rapd@net.bio.net Mon May 15 23:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.molbio.rapd Subject: Re: quality of primers, UBC vs Operon Date: 16 May 1995 05:34:49 -0700 Organization: University of Arkansas Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: <2D75D916EC@mercury.uark.edu> NNTP-Posting-Host: net.bio.net > To: rapd@net.bio.net > From: rottmann@fsl.orst.edu (William H. Rottmann) > Subject: quality of primers, UBC vs Operon > Date: Mon, 15 May 1995 14:01:02 > Our lab has noticed that more people are using Operon primers as compared to > UBC primers. Because we are concerned about the reproducibility of our > reactions, we were wondering if there was a technical reason for the apparent > preference. Is anyone aware of quality differences between primers from the > two sources that affect reproducibility, strength of bands, total yield, > background smearing? > > Will Rottmann > Forest Science > Oregn State University > > rottmann@fsl.orst.edu > We have used over 800 primers from UBC and about 20 from Operon. Initially, the reason was cost. A large collection was required for our genome scanning project and we more easily afford sets of 100 primers rather than sets of 20. I also believe Operon has done a good job of advertising whereas UBC has advertised only by email and word of mouth. We have never had any problems with the UBC primers. //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// From owner-rapd@net.bio.net Mon May 15 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!news.uoregon.edu!gaia.ucs.orst.edu!news.uidaho.edu!raven.csrv.uidaho.edu!will9369 From: will9369@raven.csrv.uidaho.edu (Williams Karren) Newsgroups: bionet.molbio.rapd Subject: public domain neuro tests Date: 11 May 1995 07:18:22 GMT Organization: University of Idaho, Moscow, Idaho Lines: 4 Distribution: world Message-ID: <3osdnu$cd@newshound.uidaho.edu> NNTP-Posting-Host: raven.csrv.uidaho.edu Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit X-Newsreader: TIN [version 1.2 PL2] I am looking for public domain neuropsych tests which have strong established norms. If you have suggestions, please let me know at will9369@uidaho.edu, thanks! From owner-rapd@net.bio.net Tue May 16 23:00:00 1995 Path: biosci!acadiau.ca!marlene.snyder From: marlene.snyder@acadiau.ca ("MARLENE SNYDER") Newsgroups: bionet.molbio.rapd Subject: UBC primer concentration Date: 17 May 1995 07:18:10 -0700 Organization: Acadia University Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <5932FA1D62@ace.acadiau.ca> NNTP-Posting-Host: net.bio.net I was very interested to read the comment about the UBC primers and the fact that concentration had to be boosted to get good banding patterns. We went to 5x the recommended concentration, and at the concentration get good results, but got negligible banding at the recommended concentration. Re-ordering specific primers is pretty inexpensive, so we haven't minded too much the fact that we go through them a little more rapidly than expected. Marty Snyder From owner-rapd@net.bio.net Tue May 16 23:00:00 1995 Path: biosci!BRADLEY.BRADLEY.EDU!rrs From: rrs@BRADLEY.BRADLEY.EDU (Robert Stephens) Newsgroups: bionet.molbio.rapd Subject: Re: UBC primer concentration Date: 17 May 1995 09:40:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <5932FA1D62@ace.acadiau.ca> NNTP-Posting-Host: net.bio.net Hi, Marty. What organism(s) do you study? Bob rrs@bradley.bradley.edu Robert Rhea Stephens Biology Department Bradley University Peoria, IL 61625 On 17 May 1995, MARLENE SNYDER wrote: > I was very interested to read the comment about the UBC primers and > the fact that concentration had to be boosted to get good banding > patterns. We went to 5x the recommended concentration, and at the > concentration get good results, but got negligible banding at the > recommended concentration. Re-ordering specific primers is pretty > inexpensive, so we haven't minded too much the fact that we go > through them a little more rapidly than expected. > > Marty Snyder > > From owner-rapd@net.bio.net Tue May 16 23:00:00 1995 Path: biosci!SERVIDOR.DGSCA.UNAM.MX!carvalho From: carvalho@SERVIDOR.DGSCA.UNAM.MX (Carvalho Torres Alexandro cassio-UACPYP) Newsgroups: bionet.molbio.rapd Subject: Re: RAPD Analysis? Date: 16 May 1995 17:09:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net On Tue, 16 May 1995, John Tudor wrote: > I have RAPD data for fourteen different strains of bacteria. > I would like to arrange them on a relatedness diagram, such > as a dendrogram. Does anyone know of a rather simple computer > program that I could use that would help me construct such > a diagram? If so, is it available at an ftp or www site? > Thanks for whatever info you can give. > > Dear Tudor, We could make a matriz, like presence of a band (1) and non-presence (0). Wiht htis results you could nalyse your data in program like SSPC. This program you could analyse your data and make phylogenetic tree. Luck Best regards. Alexxandro carvalho INNSZ. Mexico City. From owner-rapd@net.bio.net Sun May 21 23:00:00 1995 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!swrinde!pipex!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: "R.Rapley" Newsgroups: bionet.molbio.rapd Subject: RNA analysis & PCR methods courses Date: 22 May 1995 13:30:18 +0100 Lines: 47 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3pq04q$ri7@mserv1.dl.ac.uk> X-Sender: apx085@rowan MIME-Version: 1.0 Original-To: bionews@dl.ac.uk, bioforum@dl.ac.uk, methods@dl.ac.uk, rapd@dl.ac.uk, plantbio@dl.ac.uk, microbio@dl.ac.uk, sci.bio.usenet@decwrl.com, sci.bio.technology.usenet@decwrl.com RNA Extraction & Analysis ========================= 20 September 1995 & 19th December 1995 PCR Methods & Applications ========================== 21 September 1995 & 20th December 1995 Coventry University, UK These are two one day laboratory based practical courses that may be attended alone or as a two day course. They provide a basic introduction into the extraction and characterisation of RNA and the techniques and applications of the PCR. The practical component is supplemented by seminars and demonstrations in related topics such as ; Northern blotting, mRNA isolation, RPA & S1 mapping, rRNA isolation, cDNA synthesis & RT-PCR etc. for the RNA course. For the PCR course, Microbe identification, probe preparation, PCR cloning & sequencing and primer design will be among the topics. These introductory courses will be of interest to those initiating work in these areas or wishing to update their knowledge. Fees are GBP100 for each course inclusive of lunch, tea/coffee. For attendance at both courses the fee is GBP160. Further Information and Registration forms: Debbie Elliott, Commercial Development Unit, Coventry University, Priory Street, Coventry, CV1 5FB United Kingdom. Tel. 01203 838145 Fax. 01203 221396 email Dr Ralph Rapley (rapley@coventry.ac.uk) ************************************************************************* From owner-rapd@net.bio.net Sun May 21 23:00:00 1995 Path: biosci!rutgers!gatech!newsxfer.itd.umich.edu!nntp.cs.ubc.ca!cs.ubc.ca!unixg.ubc.ca!news.bc.net!rover.ucs.ualberta.ca!news From: your.id@gpu.srv.ualberta.ca (your name) Newsgroups: bionet.molbio.rapd Subject: HELP: guinea pig cDNA library needed Date: 22 May 1995 21:14:36 GMT Organization: University of Alberta Lines: 7 Message-ID: <3pqurs$1rsc@rover.ucs.ualberta.ca> Reply-To: khull@physio.med.ualberta.ca NNTP-Posting-Host: wombat.physio.med.ualberta.ca X-Newsreader: WinVN 0.92.4 I'm trying to find a guinea pig hepatic cDNA library. Stratagene doesn't have one, Clontech has one, but in a poor vector, and I don't know where else to look. Any ideas? Thanks, Kerry From owner-rapd@net.bio.net Sun May 21 23:00:00 1995 Path: biosci!LIFSCI.SDSU.EDU!MCCLELLAND From: MCCLELLAND@LIFSCI.SDSU.EDU Newsgroups: bionet.molbio.rapd Subject: postdoc Date: 22 May 1995 13:47:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: <950522060004.20200704@LIFSCI.SDSU.EDU> NNTP-Posting-Host: net.bio.net PLEASE POST Postdoctoral Fellowship Salmonella genome May 22nd 1995 Dear colleague, The primary postdoc on our Salmonella project has been offered his own laboratory at DOE/WSU. I am thus looking for someone to take over his position as soon as possible. If you know someone who might be interested I would be grateful if you could direct that person to me by e-mail to McClelland@lifsci.sdsu.edu or by FAX to 619 535 5472. The first step of this project is well advanced with 95% of the genome covered by an order and overlapping set of lambda clones. Approximately, 20% of these have been restriction mapped. We have delineated in these clones many of the known loops that distinguish Salmonella from E. coli. The most interesting part of the project is just starting in which we delineate the role of some of these lifestyle-specific regions. The next stage of the project will also involve the use of RNA fingerprinting by arbitiarily primed PCR in identify coordinately regulated genes in point mutations and large deletions in these regions (Wong K.K. and McClelland M. 1994. A stress-induced gene from Salmonella typhimurium identified by arbitrarily primed PCR of RNA (RAP). Proc. Natl. Acad. Sci. U.S.A. 91:639- 643; McClelland et al., Trends in Genetics, June 1995). Se also Wong K.K. and McClelland M. 1994. High-resolution restriction map for a 240- kilobase region spanning 91- to 96 minutes on the Salmonella typhimurium LT2 genome. J. Bacteriol. 176: 5729-5734. I am seeking someone with leadership and organizational skills who will coordinate with our collaborator Ken Sanderson and will direct a technician on the project. I would greatly appreicate your help in locating such an individual. Michael McClelland Director, Cal. Inst. Biol. Research 11099 North Torrey Pines Road La Jolla CA 92037 Phone 619 535 5486 FAX 619 535 5472 E-mail McClelland@lifsci.sdsu.edu From owner-rapd@net.bio.net Sun May 21 23:00:00 1995 Path: biosci!CORNELL.EDU!gsr1 From: gsr1@CORNELL.EDU (Geoffrey Rule) Newsgroups: bionet.molbio.rapd Subject: Re: Discriminating primers for L. monocytogenes ET types Date: 22 May 1995 11:16:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Sorry to ask , but what does ET stand for? Geoff At 3:22 PM 5/22/95, m.cooper wrote: >We have tried a range of decamers in our laboratory to attempt to type L. >momcytogenes within existing ET types. Obviously the theoretical number >of different 10 mer >primers we could look at is prohibitive and so we are keen to know if anyone >uses a primer not supplied by Operon that is particularly useful for typing. > >M. Cooper Geoffrey S. Rule Analytical Chemistry Labs NYSAES/ Cornell University Geneva, NY 14456 1-315-787-2435 From owner-rapd@net.bio.net Sun May 21 23:00:00 1995 Path: biosci!daresbury!trane.uninett.no!sunic!sunic.sunet.se!seunet!news2.swip.net!doc.news.pipex.net!pipex!uknet!strath-cs!st-and!Aberdeen!mbi049 From: mbi049@abdn.ac.uk (m.cooper) Newsgroups: bionet.molbio.rapd Subject: Discriminating primers for L. monocytogenes ET types Date: 22 May 1995 15:22:07 GMT Organization: University of Aberdeen, Scotland Lines: 6 Message-ID: <3pqa6v$c3t@nof.abdn.ac.uk> NNTP-Posting-Host: sysb.abdn.ac.uk X-Newsreader: TIN [version 1.2 PL2] We have tried a range of decamers in our laboratory to attempt to type L. momcytogenes within existing ET types. Obviously the theoretical number of different 10 mer primers we could look at is prohibitive and so we are keen to know if anyone uses a primer not supplied by Operon that is particularly useful for typing. M. Cooper From owner-rapd@net.bio.net Mon May 22 23:00:00 1995 Path: biosci!YVAX.BYU.EDU!anderswr From: anderswr@YVAX.BYU.EDU (W. Ralph Andersen) Newsgroups: bionet.molbio.rapd Subject: (none) Date: 23 May 1995 10:42:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HQUALTY5FUA23COZ@yvax.byu.edu> NNTP-Posting-Host: net.bio.net Doug R. was concerned about how some of us users of RAPD primers feel about his product and service compared to the "other" company. We have used the full slate of Operon and UBC primers during the sugarcane mapping project. In terms of the good quality of product and very satisfactory service we would highly recommend both sources to our colleagues. Ralph Andersen From owner-rapd@net.bio.net Mon May 22 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!howland.reston.ans.net!pipex!uknet!daresbury!not-for-mail From: "Miss D.A. Heipel" Newsgroups: bionet.molbio.rapd Subject: RAPDPLOT Date: 23 May 1995 10:06:46 +0100 Lines: 5 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3ps8j6$jd@mserv1.dl.ac.uk> MIME-Version: 1.0 Original-To: rapd@dl.ac.uk Does anybody know where I can get a copy of RAPDPLOT from? Diana Heipel Port Erin Marine Laboratory Port Erin, Isle of Man, UK From owner-rapd@net.bio.net Mon May 22 23:00:00 1995 Path: biosci!bloom-beacon.mit.edu!gatech!newsfeed.pitt.edu!godot.cc.duq.edu!ddsw1!vanbc.wimsey.com!news.bc.net!info.ucla.edu!library.ucla.edu!news.ucdavis.edu!dale!ez007415 From: ez007415@dale.ucdavis.edu (Marilyn Warburton) Newsgroups: bionet.molbio.rapd Subject: RAPDs vs coefficent of coancestry Date: 22 May 1995 22:37:26 GMT Organization: University of California, Davis Lines: 12 Message-ID: <3pr3n6$2r9@mark.ucdavis.edu> NNTP-Posting-Host: dale.ucdavis.edu X-Newsreader: TIN [version 1.2 PL2] Hello, netters, gotta question for you. Does anyone have citations for the latest (or any!) papers which compare genetic similarities based on RAPDs with the coefficents of coancestry (or inbreeidng coefficents) of individuals of known pedigree? there dosent seem to be a lot of work in this field... Thanks in advance for your help; Marilyn Warburton UCDavis Dept. of Pomology Davis, CA 95616 mlwarburton@ucdavis.edu From owner-rapd@net.bio.net Mon May 22 23:00:00 1995 Path: biosci!biosci!not-for-mail From: "R.Rapley" Newsgroups: bionet.announce,bionet.general,bionet.molbio.methds-reagnts,bionet.molbio.rapd,bionet.plants,bionet.microbiology,sci.bio,sci.bio.technology Subject: RNA analysis & PCR methods courses Date: 22 May 1995 17:26:05 -0700 Organization: Coventry University Lines: 45 Sender: biohelp@net.bio.net Approved: bionews-moderator@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.announce:2110 bionet.general:15336 bionet.molbio.methds-reagnts:28634 bionet.molbio.rapd:1162 bionet.plants:6884 bionet.microbiology:2314 sci.bio:16700 sci.bio.technology:3101 RNA Extraction & Analysis ========================= 20 September 1995 & 19th December 1995 PCR Methods & Applications ========================== 21 September 1995 & 20th December 1995 Coventry University, UK These are two one day laboratory based practical courses that may be attended alone or as a two day course. They provide a basic introduction into the extraction and characterisation of RNA and the techniques and applications of the PCR. The practical component is supplemented by seminars and demonstrations in related topics such as ; Northern blotting, mRNA isolation, RPA & S1 mapping, rRNA isolation, cDNA synthesis & RT-PCR etc. for the RNA course. For the PCR course, Microbe identification, probe preparation, PCR cloning & sequencing and primer design will be among the topics. These introductory courses will be of interest to those initiating work in these areas or wishing to update their knowledge. Fees are GBP100 for each course inclusive of lunch, tea/coffee. For attendance at both courses the fee is GBP160. Further Information and Registration forms: Debbie Elliott, Commercial Development Unit, Coventry University, Priory Street, Coventry, CV1 5FB United Kingdom. Tel. 01203 838145 Fax. 01203 221396 email Dr Ralph Rapley (rapley@coventry.ac.uk) ************************************************************************* From owner-rapd@net.bio.net Mon May 22 23:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.molbio.rapd Subject: Oops and Re: quality of primers, UBC vs Operon Date: 23 May 1995 11:33:58 -0700 Organization: University of Arkansas Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: <343B9F26B8@mercury.uark.edu> NNTP-Posting-Host: net.bio.net > Date: Tue, 23 May 1995 11:26:04 -0700 > From: anderswr@yvax.byu.edu (W. Ralph Andersen) > Subject: Re: quality of primers, UBC vs Operon > To: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") > Doug; We have used your all of your primers and all of Operon's primers on > our sugarcane mapping project with the sugarcane consortium for melecular > biology. We found no difference in quality or service. Both are > excellent. I would recommend you both highly. > > Sincerely yours, Ralph > > > >To: rapd@net.bio.net >From: anderswr@yvax.byu.edu (W. Ralph Andersen) >Subject: (none) >Date sent: 23 May 1995 10:42:29 -0700 > >Doug R. was concerned about how some of us users of RAPD primers feel about >his product and service compared to the "other" company. We have used the >full slate of Operon and UBC primers during the sugarcane mapping project. >In terms of the good quality of product and very satisfactory service we >would highly recommend both sources to our colleagues. Ralph Andersen First- sorry about the do nothing meaningless email that preceded, "fingers doeth without thought!!" Second- lest there be any confusion, I am not affiliated with John Hobbs, the NAPS, University of British Columbia, nor am I a spokesperson for any of these persons or entities. I have on occasion expounded on the virtues of the oligo sets from UBC but should not be construed to be in the business of producing RAPD primers or other oligos. I don't even own a synthesizer, I buy mine. No offense to Ralph or anyone else, I just want to make sure and clear this up. Go Forth and Amplify //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// From owner-rapd@net.bio.net Mon May 22 23:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.molbio.rapd Subject: Re: quality of primers, UBC vs Operon Date: 23 May 1995 11:25:30 -0700 Organization: University of Arkansas Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: <34140E4138@mercury.uark.edu> NNTP-Posting-Host: net.bio.net > Date: Tue, 23 May 1995 11:26:04 -0700 > From: anderswr@yvax.byu.edu (W. Ralph Andersen) > Subject: Re: quality of primers, UBC vs Operon > To: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") > Doug; We have used your all of your primers and all of Operon's primers on > our sugarcane mapping project with the sugarcane consortium for melecular > biology. We found no difference in quality or service. Both are > excellent. I would recommend you both highly. > > Sincerely yours, Ralph > > > To: rapd@net.bio.net From: anderswr@yvax.byu.edu (W. Ralph Andersen) Subject: (none) Date sent: 23 May 1995 10:42:29 -0700 Doug R. was concerned about how some of us users of RAPD primers feel about his product and service compared to the "other" company. We have used the full slate of Operon and UBC primers during the sugarcane mapping project. In terms of the good quality of product and very satisfactory service we would highly recommend both sources to our colleagues. Ralph Andersen //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| ||drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// From owner-rapd@net.bio.net Tue May 23 23:00:00 1995 Path: biosci!UKCC.UKY.EDU!DGMILL00 From: DGMILL00@UKCC.UKY.EDU (Don Miller) Newsgroups: bionet.molbio.rapd Subject: (none) Date: 24 May 1995 13:51:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <199505242050.NAA15234@net.bio.net> NNTP-Posting-Host: net.bio.net unsubscribe rapd-net From owner-rapd@net.bio.net Tue May 23 23:00:00 1995 Path: biosci!COSMAIL2.CTD.ORNL.GOV!ygl From: ygl@COSMAIL2.CTD.ORNL.GOV ("Lee E. Gunter") Newsgroups: bionet.molbio.rapd Subject: Re: RAPDPLOT Date: 24 May 1995 05:56:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <328.ygl@cosmail2.ctd.ornl.gov_POPMail.PC_3.2.2> Reply-To: NNTP-Posting-Host: net.bio.net On 23 May 1995 10:06:46 +0100, Miss D.A. Heipel wrote: >Does anybody know where I can get a copy of RAPDPLOT from? > >Diana Heipel >Port Erin Marine Laboratory >Port Erin, Isle of Man, UK RAPDPLOT is available through anonymous ftp from Bill Black at Colorodo State: 1. Log on to the network 2. ftp lamar.colostate.edu 3. anonymous 4. your id 5. cd pub/wcb4 6. binary 7. dir You will see a long list of compressed files which can be transferred and unzipped using the PKUNZIP program (which is, I think, available at this site if you don't already have it). Good luck! Lee E. Gunter ygl@cosmail2.ctd.ornl.gov: e-mail Environmental Sciences Division (615) 576-7697 : phone Oak Ridge National Laboratory (615) 576-9939 : fax Bldg 1506, MS-6034 P.O. Box 2008 Oak Ridge, TN 37831-6034 From owner-rapd@net.bio.net Thu May 25 23:00:00 1995 Path: biosci!VMS2.MACC.WISC.EDU!roc From: roc@VMS2.MACC.WISC.EDU (Raul O. Castillo) Newsgroups: bionet.molbio.rapd Subject: Re: Use of RAPD for somaclonal variation... Date: 26 May 1995 16:14:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 63 Sender: daemon@net.bio.net Distribution: world Message-ID: <25052618141154@vms2.macc.wisc.edu> NNTP-Posting-Host: net.bio.net Date: Fri, 26 May 95 16:33:16 , n.c.pancholi@reading.ac.uk: NARESH PANCHOLI: You might be working with mitochondrial amplified sequences, that is why you are getting different polymorphisms. I also assume you have the same genotype for every treatment, otherwise that might be the problem. Are you tested if the primers are sampling nuclear genome? How about ploidy level, remember the dominance character of the RAPDs? Just some thoughts, I hope it is not complicating more. Cheers, Raul O. Castillo ROC@macc.wisc.edu U. of Wisconsin, Horticulture, 1575 Linden Dr., Madison, WI 53706 Tel. 608-262 7406, Fax 608-262 4743 """"""""""""""""""""""""""""""""""" -----> After june 30/95: Raul O. Castillo castillo@fitogen.sc.iniap.gov.ec NATIONAL DEPARTMENT OF PLANT GENETIC RESOURCES (DENAREF)-INIAP Casilla 17-01-340 Quito-Ecuador Tel. (593-2) 690 691/2/3/4, (593-2) 650 042 FAX: (593-2) 504 240 and 593-2-690-691 From owner-rapd@net.bio.net Thu May 25 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: BANANA & PLANTAIN Newsgroups: bionet.molbio.rapd Subject: Use of RAPD for somaclonal variation... Date: 26 May 1995 21:23:21 +0100 Lines: 40 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <3q5dbp$7a5@mserv1.dl.ac.uk> MIME-Version: 1.0 Original-To: RAPD , Plant Biology , Methods-and-Reagents Hello Netters, I used RAPD for the detection of somaclonal variation in in vitro grown banana plants. I compared plants grown in solid medium (i.e., solidified with Gelrite) or liquid medium. Also, I tested 2 different cytokinins. The objective was to find the variation generated by different system and the degree of variation, if there is any. Surprisingly, significant somaclonal variation between 2 different media as well as cytokinins was observed. I found varied band intensity among variant samples, apart from extra or missing bands. My question is whether this change in band intensity (which was reproducible, so at least it is not because of RAPD parameters) is really associated with the presence of somaclonal variation? If so, then what is the possible explanation? One in my mind is that this indicates an alteration in the copy number of gene involved. Has anybody observed similar phenomena? Any pointer in this direction will be highly appreciated. Thanks. Posted to: bionet.molbio.rapd bionet.plants bionet.molbio.methds-reagnts ------------------------------------------------------------------------- NARESH PANCHOLI AGRICULTURAL BOTANY PHONE: +44 1734 875123 EXT. 4087 SCHOOL OF PLANT SCIENCES FAX: +44 1734 316577 THE UNIVERSITY OF READING READING RG6 6AS ENGLAND N.C.PANCHOLI@READING.AC.UK ------------------------------------------------------------------------- From owner-rapd@net.bio.net Sun May 28 23:00:00 1995 Path: biosci!SERVIDOR.DGSCA.UNAM.MX!carvalho From: carvalho@SERVIDOR.DGSCA.UNAM.MX (Carvalho Torres Alexandro cassio-UACPYP) Newsgroups: bionet.molbio.rapd Subject: Re: Discriminating primers for L. monocytogenes ET types Date: 29 May 1995 15:08:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <3pqa6v$c3t@nof.abdn.ac.uk> NNTP-Posting-Host: net.bio.net Dear Cooper, Try teh primers descibed by Mazurier et al., I can't remember the year. I think is 1992. Betst regards, Alexandro carvalho Mexico City