From owner-rapd@net.bio.net Mon Oct 02 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.starnet.net!wupost!nic.smsu.edu!newsdist.tc.umn.edu!alpha.mrs.umn.edu!markvo@cda.mrs.umn.edu From: "Christopher T. Cole" Newsgroups: bionet.molbio.rapd Subject: Re: DNA Quantitation Date: Mon, 2 Oct 95 11:53:00 CDT Organization: University of Minnesota - Morris Lines: 35 Message-ID: <55623.colect@caa.mrs.umn.edu> Reply-To: NNTP-Posting-Host: sci242-cc.mrs.umn.edu X-Minuet-Version: Minuet1.0_Beta_17A X-POPMail-Charset: English To: rzebell@sfsu.edu On 19 Sep 1995 17:40:13 GMT, Randy K. Zebell wrote: >Does anyone have an opinion about Hoefer Scientific Instrument's DyNA >Quant 200 flourometer? Given our small lab, it seems appropriate for >rapidly quantifying DNA without the added size and expense of a >spectrophotometer. I'm wondering how accurate and reliable the instrument >is in practice. > Mr. Zebell: we have been using one of these during the last couple months and are quite happy with it. We measure genomic (plant) DNA preps, typically in the range of 2-400 ng in a 2 ml cuvette. We also have the little adapter to read samples in capillary tubes but that has not proven practical or (fortunately) necessary. I have not tried their special mirrored cuvette for reading extraordinarily tiny amounts of DNA. Our readings are quite consistent from one day to another. On the other hand, we find that the excitation curve is more non-linear than we expected. For example, if we read that a solution is 200 ng/ml, and want to dilute it down to 10 ng/ml, we have to apply an empirically derived multiplier to get the correct dilution. We have not played around with this enough to determine the cause (mainly because we do not have sufficiently accurate alternative ways of measuring DNA concentration, since spec. is influenced by RNA present and our store-bought standards have prokayotic AT/GC compositions). The machine could not be easier or quicker to use. I leave it in the hands of undergraduates for days, and they produce good results. A spec would have a lot more flexibility, if you wanted it. Good luck. --Chris Cole From owner-rapd@net.bio.net Tue Oct 03 23:00:00 1995 Path: biosci!bath.ac.uk!J.J.Dooley From: J.J.Dooley@bath.ac.uk (J J Dooley) Newsgroups: bionet.molbio.rapd Subject: membership Date: 4 Oct 1995 14:33:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510040928.aa26074@ss1.bath.ac.uk> NNTP-Posting-Host: net.bio.net Dear Sir, could you tell me how I could get registered to recieve mail from the RAPD noticeboard many thanks john dooley From owner-rapd@net.bio.net Thu Oct 05 23:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!news.sprintlink.net!simtel!swidir.switch.ch!scsing.switch.ch!news.belwue.de!fu-berlin.de!bbadd.dialup.fu-berlin.DE!not-for-mail From: bbadd@zedat.fu-berlin.de () Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.rapd Subject: RAPD and capillary electrophoresis Date: Sat, 07 Oct 1995 03:47:51 GMT Organization: Freie Universitaet Berlin Lines: 11 Message-ID: <4540u2$954@fu-berlin.de> NNTP-Posting-Host: bbadd.dialup.fu-berlin.de (160.45.218.83) X-Access: 16 17 19 X-Newsreader: Forte Free Agent v0.46 Xref: biosci bionet.molbio.methds-reagnts:34554 bionet.molbio.rapd:1312 Hey all, have anyone experience with the use of capillary electrophoresis for separation and detection of RAPD fragments? How time consuming and reproducible is that? Thanks for all answers; With greetings from Berlin Olaf ,,, (o o) ---------------------------------------------------oOO--(_)--OOo------------ From owner-rapd@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!bcm.tmc.edu!pendragon.jsc.nasa.gov!news.msfc.nasa.gov!newsfeed.internetmci.com!info.ucla.edu!news.ucdavis.edu!jerome.ucdavis.edu!user From: jfrigot@ucdavis.edu (Jerome Rigot) Newsgroups: bionet.molbio.rapd Subject: Reliable soil DNA extraction method Date: 10 Oct 1995 18:27:33 GMT Organization: UCDavis Lines: 15 Message-ID: NNTP-Posting-Host: jerome.ucdavis.edu Hello, I am looking for a reliable soil DNA extraction method that would deal with the interference problems due to humic acids. Are there methods that would be specific to filamentous fungi DNA? Thanks for your help, -- Jerome Rigot Dept. Environmental Toxicology University of California Davis, CA 95616 (916)752-5896 e-mail: jfrigot@ucdavis.edu From owner-rapd@net.bio.net Mon Oct 09 23:00:00 1995 Path: biosci!DOC.MEDIC.MIE-U.AC.JP!h-makoto From: h-makoto@DOC.MEDIC.MIE-U.AC.JP (Makoto Hirai) Newsgroups: bionet.molbio.rapd Subject: (none) Date: 10 Oct 1995 06:01:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510101301.WAA17522@doc.medic.mie-u.ac.jp> NNTP-Posting-Host: net.bio.net unsubscribe to rapd *************************** Makoto Hirai Department of Medical zoology School of Medicine Mie Univ Edobashi 2-174, Tsu, 514, Japan E-mail: h-makoto@doc.medic.mie-u.ac.jp TEL: 0592-33-2692 FAX: 0592-33-2692 *************************** From owner-rapd@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!ml.csiro.au!Chris.Bolch From: Chris.Bolch@ml.csiro.au (Christopher Bolch) Newsgroups: bionet.molbio.rapd Subject: Garbage postings Date: 11 Oct 1995 16:23:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 43 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510112321.KAA10681@aqueous.ml.csiro.au> NNTP-Posting-Host: net.bio.net Dear RAPD subscribers, I have been bombarded by a lot of "junk e-mail" from this list over the past few weeks. It culminated this morning with 5 posts from an elena foshay. What is really irritating is that the return addresses appear to be bogus so that I can't even get the satisfaction of telling to sod-off. I'm sure that most of you are just as pissed off as I am by this. I am now seriously considering unsubscribing from what is a very good, active discussion group. This the only way I can see of temporarily keeping the CR..P out of our site at Hobart. This type of activity will eventually kill groups like this if it gets unmanageable. If anyone has any ideas on how to combat this rising incidence of chain letters and other irrelevent drivel let me know. Chris ________________________________________ Any views, statements of intent, or comment in this letter have no official status and do not constitute or imply any contract with myself or my employer. ________________________________________ Christopher J. S. Bolch, Algal Ecologist/Geneticist, CSIRO Division of Fisheries, GPO Box 1538, Hobart, Tasmania, Australia, 7001. Australia PH. 002 325314 FAX 002-325000 International PH. 061-02-325314 FAX. 061-02-325000 ________________________________________ From owner-rapd@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!simtel!zombie.ncsc.mil!cs.umd.edu!haven.umd.edu!purdue!news.bu.edu!transfer.stratus.com!news3.near.net!yale!news.ycc.yale.edu!news From: Greg Fitzgerald Newsgroups: bionet.molbio.hiv,bionet.molbio.methds-reagnts,bionet.molbio.molluscs,bionet.molbio.proteins,bionet.molbio.rapd Subject: Re: Make a $1,000,000 in 90 Days! Date: 11 Oct 1995 16:58:50 GMT Organization: Yale University Biology Department Lines: 3 Message-ID: <45gt4a$flf@news.ycc.yale.edu> References: <45ge3g$o1j$5@mhafn.production.compuserve.com> NNTP-Posting-Host: 130.132.107.28 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: 102771.3337@CompuServe.COM X-URL: news:45ge3g$o1j$5@mhafn.production.compuserve.com Xref: biosci bionet.molbio.hiv:1675 bionet.molbio.methds-reagnts:34741 bionet.molbio.molluscs:172 bionet.molbio.proteins:5905 bionet.molbio.rapd:1324 Get out of this site you fool! From owner-rapd@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!simtel!zombie.ncsc.mil!cs.umd.edu!haven.umd.edu!purdue!news.bu.edu!transfer.stratus.com!news3.near.net!yale!news.ycc.yale.edu!news From: Greg Fitzgerald Newsgroups: bionet.molbio.hiv,bionet.molbio.methds-reagnts,bionet.molbio.molluscs,bionet.molbio.proteins,bionet.molbio.rapd Subject: Re: Make a $1,000,000 in 90 Days! Date: 11 Oct 1995 16:58:39 GMT Organization: Yale University Biology Department Lines: 3 Message-ID: <45gt3v$flf@news.ycc.yale.edu> References: <45ge3g$o1j$5@mhafn.production.compuserve.com> NNTP-Posting-Host: 130.132.107.28 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) To: 102771.3337@CompuServe.COM X-URL: news:45ge3g$o1j$5@mhafn.production.compuserve.com Xref: biosci bionet.molbio.hiv:1674 bionet.molbio.methds-reagnts:34740 bionet.molbio.molluscs:171 bionet.molbio.proteins:5904 bionet.molbio.rapd:1323 Get out of this site you fool! From owner-rapd@net.bio.net Tue Oct 10 23:00:00 1995 Path: biosci!citi2.fr!bollet From: bollet@citi2.fr (Claude Bollet) Newsgroups: bionet.molbio.rapd Subject: soil DNA extraction/J.RIGOT Date: 11 Oct 1995 02:46:22 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510110941.AA07854@bisance.citi2.fr> NNTP-Posting-Host: net.bio.net For small amounts, you should try micro-wave oven extraction method (Nucleic Acids Res., 1991, 19, 1955). Good luck, -- claude bollet AFCOPAT 249 bld sainte marguerite 13009 Marseille - France tel (33) 91 74 50 23 fax (33) 91 74 60 44 lab (33) 91 74 50 52 From owner-rapd@net.bio.net Wed Oct 11 23:00:00 1995 Path: biosci!LIFSCI.SDSU.EDU!MCCLELLAND From: MCCLELLAND@LIFSCI.SDSU.EDU (by way of Chris.Bolch@ml.csiro.au (Christopher Bolch)) Newsgroups: bionet.molbio.rapd Subject: RE: Garbage postings Date: 11 Oct 1995 20:29:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510120327.OAA28804@aqueous.ml.csiro.au> NNTP-Posting-Host: net.bio.net A suggestion for other RAPD subscribers who would like to give it to the postee of rubbish from Michael McClelland: Send mail to postmaster@ the address. Try various parts like postmaster@uiuc.edu. I have been doing this as a matter of course for all my junk mail. Be polite. the person reading it will be a system administrator. From owner-rapd@net.bio.net Wed Oct 11 23:00:00 1995 Path: biosci!WAIKATO.AC.NZ!marys From: marys@WAIKATO.AC.NZ (Mary Skotnicki) Newsgroups: bionet.molbio.rapd Subject: Re: Garbage postings Date: 11 Oct 1995 18:11:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I usually just discard junk messages, but I too was really fed up with receiving twenty messages from Elena Foshay, and with my response not bein accepted by her email address. So I checked her out (student at University of Illinois, with University email address) using Netscape, and remailed copies of her three messages to the Senior Computer Programmer there with a request to stop her sending out more postings. Let's hope that stops at least this one prolific individual. Christopher Bolch wrote: >I have been bombarded by a lot of "junk e-mail" from this list over the >past few weeks. It culminated this morning with 5 posts from an elena >foshay. What is really irritating is that the return addresses appear to >be bogus so that I can't even get the satisfaction of telling to sod-off. >I'm sure that most of you are just as pissed off as I am by this. > >If anyone has any ideas on how to combat this rising incidence of chain >letters and other irrelevent drivel let me know. > Mary L. Skotnicki Dept. of Biological Sciences School of Science and Technology University of Waikato Hamilton New Zealand marys@waikato.ac.nz From owner-rapd@net.bio.net Wed Oct 11 23:00:00 1995 Path: biosci!ACAD.URSINUS.EDU!kgoddard From: kgoddard@ACAD.URSINUS.EDU Newsgroups: bionet.molbio.rapd Subject: descriibe Date: 11 Oct 1995 14:32:37 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <00997B7A.C45A2900.22@acad.ursinus.edu> please delete me from this network. From owner-rapd@net.bio.net Wed Oct 11 23:00:00 1995 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!howland.reston.ans.net!swrinde!emory!news.cc.emory.edu!usenet From: Brad Thomas Newsgroups: bionet.molbio.hiv,bionet.molbio.methds-reagnts,bionet.molbio.molluscs,bionet.molbio.proteins,bionet.molbio.rapd Subject: Re: Make a $1,000,000 in 90 Days! Date: 11 Oct 1995 23:51:55 GMT Organization: Emory University Lines: 4 Message-ID: <45hlar$894@moe.cc.emory.edu> References: <45ge3g$o1j$5@mhafn.production.compuserve.com> NNTP-Posting-Host: alvin.gen.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Macintosh; I; 68K) X-URL: news:45ge3g$o1j$5@mhafn.production.compuserve.com Xref: biosci bionet.molbio.hiv:1676 bionet.molbio.methds-reagnts:34766 bionet.molbio.molluscs:173 bionet.molbio.proteins:5915 bionet.molbio.rapd:1326 Does this have something to do with smuggling aliens ? (Sorry for the bandwidth) From owner-rapd@net.bio.net Wed Oct 11 23:00:00 1995 Path: biosci!ml.csiro.au!Chris.Bolch From: Chris.Bolch@ml.csiro.au (Christopher Bolch) Newsgroups: bionet.molbio.rapd Subject: RE: Garbage postings Date: 11 Oct 1995 20:53:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510120351.OAA00936@aqueous.ml.csiro.au> NNTP-Posting-Host: net.bio.net Sorry Michael and RAPD netters, T he introductory sentence is not meant to mean that rubbish is posted from Mike McClelland . A full stop went missing in there somewhere. What I meant is the suggestion came from Michael McClelland Chris From owner-rapd@net.bio.net Wed Oct 11 23:00:00 1995 Path: biosci!ml.csiro.au!Chris.Bolch From: Chris.Bolch@ml.csiro.au (Christopher Bolch) Newsgroups: bionet.molbio.rapd Subject: RE: Garbage postings Date: 12 Oct 1995 15:53:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510122250.JAA19901@aqueous.ml.csiro.au> NNTP-Posting-Host: net.bio.net A good suggestion for reducing junk mail is below. I will mail it to the bionet administrator, anyone know the address? >From: BIO_HANNAN@emuvax.emich.edu >Date: Wed, 11 Oct 1995 21:35:07 -0400 (EDT) >To: Chris.Bolch@aqueous.ml.csiro.au >Subject: RE: Garbage postings > >It is possible to allow postings only from persons who have subscribed to the >group. At least that might sovle the accountability problem ,and allow >others to flame spammers. > >Gary Hannan > > ________________________________________ Any views, statements of intent, or comment in this letter have no official status and do not constitute or imply any contract with myself or my employer. ________________________________________ Christopher J. S. Bolch, Algal Ecologist/Geneticist, CSIRO Division of Fisheries, GPO Box 1538, Hobart, Tasmania, Australia, 7001. Australia PH. 002 325314 FAX 002-325000 International PH. 061-02-325314 FAX. 061-02-325000 ________________________________________ From owner-rapd@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!ASRR.ARSUSDA.GOV!abartlet From: abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") Newsgroups: bionet.molbio.rapd Subject: Re: Garbage postings Date: 13 Oct 1995 11:51:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: References: Along with the rest of you, I am disturbed by the posting of garbage by people like Elena Foshay. The addresses she used were all in Scottsdale, Arizona, so I checked them out. There are no legitimate businesses listed in Scottsdale under any of the names posted. These are obviously boilerplate addresses. I have made copies of the postings and plan to go to the address to register my objection to the use of the net for such postings. I appreciate the fact that Mary was able to get to the Foshay computer. All of my flames bounced back. We have to get this stopped because it is becoming a plague. Alan C. Bartlett "GENES-R-US" "ALTERATIONS AVAILABLE!^" "^some restrictions may apply:)" From owner-rapd@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!IGC.APC.ORG!uspugwash From: uspugwash@IGC.APC.ORG (Student Pugwash USA) Newsgroups: bionet.molbio.rapd Subject: Nobel Peace Prize to Pugwash Conferences Date: 13 Oct 1995 13:13:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510132010.NAA16379@igc4.igc.apc.org> (With apologies for duplicate postings...) ----------------NEWS RELEASE------------------- Contact: Sandra J. Ionno 202-393-6555 For immediate release October 13, 1995 Student Pugwash USA Commends Awarding of Nobel Prize to Professor Rotblat and Pugwash Conferences (Washington, DC)--Student Pugwash USA celebrates the award of the Nobel Peace Prize to Professor Joseph Rotblat and the Pugwash Conferences on Science and World Affairs. "The work of Professor Rotblat and the Pugwash Conferences has inspired a vital student movement across the United States and around the world," Sandra J. Ionno, Executive Director of Student Pugwash USA said earlier today. Founded in 1979, Student Pugwash USA is modeled after the Pugwash Conferences on Science and World Affairs, which began in 1957 in Pugwash, Nova Scotia. Student Pugwash USA draws on the Pugwash tradition as it prepares students to meet the challenges they will face as global citizens and decision-makers in the 21st century. "Awarding the Nobel Peace Prize to Professor Rotblat and Pugwash demonstrates vividly that individual responsibility is the first step on the pathway to peace," Ionno added. "The renewed French and Chinese nuclear testing programs prove the need for ethics to play a greater role in scientific decision-making if we are going to make the world a truly safer, more secure place," she said. Albert Einstein, Bertrand Russell, and award-winner Joseph Rotblat were among the signers in 1955 of a manifesto urging scientists to consider the social, moral, and ethical implications of weapons of mass destruction. The Pugwash spirit has always implied the need for scientists to broadly consider the ethical implications of their work. Student Pugwash USA has chapters on 50 campuses across the United States and is one of 24 national Student/Young Pugwash groups around the world. -30- From owner-rapd@net.bio.net Thu Oct 12 23:00:00 1995 Path: biosci!ACD.TUSK.EDU!Prakash From: Prakash@ACD.TUSK.EDU (C. S. Prakash) Newsgroups: bionet.molbio.rapd Subject: Pugwash wins Nobel Peace Prize Date: 13 Oct 1995 07:58:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 43 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HWDYKIYQAQ000C6O@Acd.Tusk.Edu> Pugwash International organization and its present leader Joesph Rodblatt who won the Nobel Peace Prize are little known. The organization derives its name from the Pugwash village in Nova Scotia where "Pugwash Manifesto" was developed by a group of visionary thinkers in 1955 including Albert Einstein, Bertrand Russell, Linus Pauling and Jonas Salk. The manifesto urged citizens of the world to fufill their moral obligation to deal with the danger of nuclear weapons. Pugwash has been very instrumental in ending the coldwar. Student Pugwash USA, based in Washington, DC is related to the Pugwash International. However, the Student Pugwash deals with broader scientific and societal issues, and attempts to foster a dialogue among students on issues such as societal impacts of human genome mapping, reprodutive technologies, agricultural biotechnology, energy etc at US college campuses. It holds a conference once in two years, invites students from across the globe and also many leaders from academia, administration and industry. Student Pugwash chapters in campuses are actively promoting such discussions. I was invited as a student participant in a conference during 1985 at Princeton University and then was a facilitator in 1987 at Stanford University. Student Pugwash does not take stand on issues and is a great forum for young scientists to meet other like minded people and to discuss issues on how our research affects the world. Those interested in receiving more information about Student Pugwash may contact Student Pugwash USA 815, 15 st NW, Suite 814 Washington, DC 20815 1-800-969-2784 Fax: (202) 797-4664 Internet: uspugwash@igc.apc.org C. S. Prakash Voice (334) 727 - 8023 Tuskegee University Fax (334) 727 - 8552 School of Agriculture Email: Prakash@Acd.Tusk.Edu Tuskegee, Alabama 36088 USA From owner-rapd@net.bio.net Sat Oct 14 23:00:00 1995 Path: biosci!daresbury!not-for-mail From: "Miss D.A. Heipel" Newsgroups: bionet.molbio.rapd Subject: RAPDPLOT/PHYLIP Date: 15 Oct 1995 22:05:14 +0100 Lines: 9 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <45rt2a$lol@mserv1.dl.ac.uk> MIME-Version: 1.0 Original-To: rapd@dl.ac.uk I am trying to analyse RAPD-data using the programs RAPDPLOT and the PHYLIP-package. The matrices produced by RAPDPLOT seem to ok, but when I run them in the Neighbor-program in PHYLIP to construct an UPGMA tree, it gives the message 'argument singularity in log'. Does anybody know what that means? Maybe someone could send me another version of PHYLIP? Thanks, Diana From owner-rapd@net.bio.net Sat Oct 14 23:00:00 1995 Path: biosci!ml.csiro.au!Chris.Bolch From: Chris.Bolch@ml.csiro.au (Christopher Bolch) Newsgroups: bionet.molbio.rapd Subject: Re: Chain letters and junk mail on RAPD net Date: 15 Oct 1995 15:44:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 76 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510152242.JAA09290@aqueous.ml.csiro.au> NNTP-Posting-Host: net.bio.net >Sender: BIOSCI Administrator >Date: Fri, 13 Oct 95 10:59:21 PDT >From: BIOSCI Administrator >Reply-To: biosci-help@net.bio.net >To: Chris.Bolch@aqueous.ml.csiro.au (Christopher Bolch) >Subject: Re: Chain letters and junk mail on RAPD net > >> Dear bionet helper, >> >> There has been asharp increase in the amount of unsolicited junk mail and >> chain letters arrive in the last few weeks. Most of it has come to me >> through" rapd@net.bio.net". Often the mail is sent from bogus addresses so >> I cannot even reply to ask then to desist. True commercial interests >> usually only have to be told once and they stop. > >Right. It is getting useless to protest these things. I just posted >a note on bionet.general about similar issues. > >> I think most of us do not mind individuals or groups who are advertising >> relevent molecular biology products which are novel, unavailable >> commercially, or are being offered at a substantially reduced rate (e.g. >> Some of the University groups offering oligo synthesis or particular PCR >> primers etc) > >We do not even permit these. Opening the door to these posts would >set a bad precedent - again see bionet.general. > >> A copy of my correspondence to the list and a reply from Gary Hannan is >> shown below. Gary had what I thought was a viable means of stemming the >> tide or garbage. Is it possible to establish a setup so that only >> subscribers to the list, with real addresses can, mail to the list? At >> least then the users could track down the culprit and tell them to go away. >> Network administrators could then also remove their registration if >> necessary. > >Gary's idea is not new. We proposed this ourselves early this year >but did not meet with a great response at the time. It also entails >additional software development both here and at our Daresbury site >and additional ongoing maintenance as people change addresses. The >easiest way to cut this down is if someone, usually the current group >discussion leader, wants to moderate the newsgroup. You might want to >start a discussion about finding a moderator on the rapd newsgroup. >If someone wants to step forward we can convert the group and stop >most of the spams. > > Sincerely, > > Dave Kristofferson > BIOSCI/bionet Manager > > biosci-help@net.bio.net > > ________________________________________ Any views, statements of intent, or comment in this letter have no official status and do not constitute or imply any contract with myself or my employer. ________________________________________ Christopher J. S. Bolch, Algal Ecologist/Geneticist, CSIRO Division of Fisheries, GPO Box 1538, Hobart, Tasmania, Australia, 7001. Australia PH. 002 325314 FAX 002-325000 International PH. 061-02-325314 FAX. 061-02-325000 ________________________________________ From owner-rapd@net.bio.net Mon Oct 16 23:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: IMPORTANT: BIOSCI miniFAQ Date: 17 Oct 1995 02:02:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 196 Sender: daemon@net.bio.net Distribution: world Message-ID: <199510170900.CAA08986@net.bio.net> NNTP-Posting-Host: net.bio.net This is a new "miniFAQ" designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) What to do about "spams," i.e., junk mail, ads, etc. 2) Examples of subscribing and unsubscribing to the mailing lists. 3) How to access BIOSCI/bionet newsgroup archives. 4) The BIOSCI user address and research interest directory. 1) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups) and mailing lists. The same postings are distributed on both media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the newsgroups from about 95% of the spams that are being sent to date. This means that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings. Unfortunately there are easy ways for determined spammers to override the moderation mechanism. We are working on new systems to provide access to our newsgroups over the WWW. These should be available soon, probably November 1995, and will allow you to use your Web browser to look at the news postings. While this will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. 2) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 3) How to access BIOSCI/bionet newsgroup archives. -------------------------------------------------- Back postings of all BIOSCI/bionet newsgroups can be found on the World Wide Web at URL http://www.bio.net/. There are several searchable newsgroup indices at this site. E-mail users can search the BIOSCI archives by using our waismail e-mail server. For instructions send the message help to waismail@net.bio.net. Leave the Subject: line blank (anything entered on the Subject: line is ignored). 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-rapd@net.bio.net Wed Oct 18 23:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!info.ucla.edu!agate!news.ucdavis.edu!dale!ez026048 From: ez026048@dale.ucdavis.edu (Hong Lin) Newsgroups: bionet.molbio.rapd Subject: Diversity Date: 18 Oct 1995 04:39:40 GMT Organization: University of California, Davis Lines: 3 Message-ID: <4620ec$a0u@mark.ucdavis.edu> NNTP-Posting-Host: dale.ucdavis.edu Summary: puzzle: X-Newsreader: TIN [version 1.2 PL2] Keywords: From owner-rapd@net.bio.net Sat Oct 21 23:00:00 1995 Path: biosci!agate!howland.reston.ans.net!news.sprintlink.net!simtel!zombie.ncsc.mil!cs.umd.edu!haven.umd.edu!purdue!lerc.nasa.gov!magnus.acs.ohio-state.edu!infoserver.bgsu.edu!bgnet.bgsu.edu!pmorris From: pmorris@bgnet.bgsu.edu (Paul Francis Morris) Newsgroups: bionet.molbio.rapd Subject: very small rapd band Date: 22 Oct 1995 23:31:38 GMT Organization: Bowling Green State University, Bowling Green, OH (USA) Lines: 15 Message-ID: <46ek8q$6ec@infoserver.bgsu.edu> Reply-To: pmorris@bgnet.bgsu.edu (Paul Francis Morris) NNTP-Posting-Host: bgnet1.bgsu.edu I am having some difficulty getting RAPD's to work in a new lab, on a new machine (LC2400, using a new protocol. The only band that I see on 2% agarose gels is one that runs below the bromophenol blue marker i.e.: less than 50-100bp. I am using the Stoffel fragment and a MgCl of 2.5 mM. One thought that I had was that perhaps I don't have sufficient melting of the template DNA (first melt 75 sec.). Another idea that I have is that the ratio of the random primers to the fungal DNA concentration is too high and I should reduce the concentration of the Operon primer I am using (OPC10). This primer has worked reliably in the past which is why I started with it here. The sequence of OPC-10 is TGTCTGGGTG if that is informative. In previous work I have found that I got best amplification with very small amounts of this fungal DNA usually less than 2.5 ng total/25 ul. Furthur suggestions or guesses are welcome. From owner-rapd@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!ACD1.BYU.EDU!FARMERJ From: FARMERJ@ACD1.BYU.EDU (James Farmer) Newsgroups: bionet.molbio.rapd Subject: moderation of rapd usergroup Date: 23 Oct 1995 09:08:32 -0700 Organization: Brigham Young University Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: I just returned form a week at a meeting and found a lot of e-mail, including three on commercial postings to bionet. I guess I am still the discussion leader for rapd, so before someone proposes that we move to a moderated format to eliminate commercial postings, let me make this one comment. I would be willing to be a moderator, or I would be willing to transfer that responsibility to someone else. Just remember that in trade for fewer commercial postings you give up response time. I would only be willing to check the postings once or twice a day, and I would often be away from the office on weekends and for a day or so at other times. I assume that I could transfer the moderator duties to someone else when I leave town for an extended period. That means we would nearly always have to wait a day for a response to a question. I think that delays might be more obnoxious for many people than the occasional commercial message. You can delete commercials in a few seconds, but you can't speed up the moderation. I suggest that we continue as at present unless the commercial problem becomes considerably worse. Nonetheless, I will go with the majority if you all feel otherwise. James Farmer farmerj@byu.edu From owner-rapd@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!news00.sunet.se!sunic!news99.sunet.se!columba.udac.uu.se!populus.slu.se!newsmgr From: Alfred.Szmidt@genfys.slu.se (Alfred E. Szmidt) Newsgroups: bionet.molbio.rapd Subject: Re: RAPDPLOT/PHYLIP Date: 23 Oct 1995 21:18:31 GMT Organization: SLU, Umeå Lines: 11 Distribution: bionet Message-ID: <46h0r7$hce@populus.slu.se> References: <45rt2a$lol@mserv1.dl.ac.uk> NNTP-Posting-Host: b203.genfys.slu.se Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 Could you first indicate which version are you using now? Best wishes, Alfred E. Szmidt In article <45rt2a$lol@mserv1.dl.ac.uk>, D.A.Heipel@liverpool.ac.uk says... > >I am trying to analyse RAPD-data using the programs RAPDPLOT and >the PHYLIP-package. From owner-rapd@net.bio.net Sun Oct 22 22:00:00 1995 Path: biosci!ASRR.ARSUSDA.GOV!abartlet From: abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") Newsgroups: bionet.molbio.rapd Subject: Re: very small rapd band Date: 23 Oct 1995 08:10:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <46ek8q$6ec@infoserver.bgsu.edu> I have found two things that caused previously successful rapd bands to disappear. I had a bad batch of dNTP's from Perkin-Elmer that gave very sporadic results, but mostly blank lanes. We recently became much more efficient in extracting DNA and found out that we had to cut the amount of the DNA aliquot in half in order to make our reactions work. Perhaps your new protocol gives you better DNA. I have been told that the low molecular weight band you describe is composed of oligos of the primer. That seems correct since it seems to always appear in blank lanes, but I haven't sequenced it to actually see. Alan C. Bartlett "GENES-R-US" "ALTERATIONS AVAILABLE!^" "^some restrictions may apply:)" From owner-rapd@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!ASRR.ARSUSDA.GOV!abartlet From: abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") Newsgroups: bionet.molbio.rapd Subject: Re: moderation of rapd usergroup Date: 24 Oct 1995 08:33:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: References: Although I am ordinarily a very moderate person, in this case I would vote for non-moderation. People have suggested other remedies which seem to me more appropriate. I am pursuing a complaint with the postmaster general concerning a couple of the recent chainletter posting we have had. The local postmaster had no sympathy with me since he claimed no responsibility for the internet. However, I still feel that since the perpetrators are asking us to use the mail to obtain their services and to foster their Ponzi schemes, the Post Office should be involved. Deep in my heart I know they won't get involved, but I am so ticked off at these slimeballs that I feel a need to try. Alan C. Bartlett "GENES-R-US" "ALTERATIONS AVAILABLE!^" "^some restrictions may apply:)" From owner-rapd@net.bio.net Tue Oct 24 22:00:00 1995 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!hamblin.math.byu.edu!usenet From: farmerj Newsgroups: bionet.molbio.rapd Subject: Has anyone tried BRL AFLP kit on Drosophila? Date: 24 Oct 1995 22:31:50 GMT Organization: Brigham Young University Lines: 8 Message-ID: <46jpgm$9en@hamblin.math.byu.edu> NNTP-Posting-Host: farmerj.byu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 32bit) I am considering AFLP as a supplement to RAPD and PCR. However, BRL says the DNA must be very pure. Since I use DNA from a single fly, I can't do a whole lot of purification. Has anyone tried AFLP on crude insect DNA? James Farmer Brigham Young University From owner-rapd@net.bio.net Thu Oct 26 22:00:00 1995 Path: biosci!rutgers!oitnews.harvard.edu!fas-news.harvard.edu!husc7!crcortes From: crcortes@husc7.harvard.edu (C Cortese) Newsgroups: bionet.molbio.rapd Subject: RAPDPLOT and PHYLIP Date: 27 Oct 1995 13:07:25 GMT Organization: Harvard University, Cambridge, Massachusetts Lines: 3 Message-ID: <46qlid$2gp@decaxp.harvard.edu> NNTP-Posting-Host: husc7.harvard.edu X-Newsreader: TIN [version 1.2 PL2] I use PHYLIP, but was not aware of RAPDPLOT. Is it available by ftp? crc From owner-rapd@net.bio.net Thu Oct 26 22:00:00 1995 Path: biosci!SERVIDOR.DGSCA.UNAM.MX!carvalho From: carvalho@SERVIDOR.DGSCA.UNAM.MX (Carvalho Torres Alexandro cassio-UACPYP) Newsgroups: bionet.molbio.rapd Subject: (none) Date: 27 Oct 1995 07:39:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: ssssss From owner-rapd@net.bio.net Fri Oct 27 22:00:00 1995 Path: biosci!IRRI.CGNET.COM!NHUANG From: NHUANG@IRRI.CGNET.COM Newsgroups: bionet.molbio.rapd Subject: RAPD, AFLP and varietal identification Date: 28 Oct 1995 01:05:17 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HWZ9WZ3R8W000U2N@irri.cgnet.com> NNTP-Posting-Host: net.bio.net Dear netter, We are interested to have a standardized DNA fingerprinting procedure and standard for rice varietal identification. We are troubled with low reproducibility of RAPD, low polymorphism of STS (PCR). Is the AFLP the way to go? Are there any accepted standard for varietal identification? If yes, can you tell me where to find it? If no, do we need one and how to establish one? Thanks. Ning Huang From owner-rapd@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!FCCC.EDU!jc_pugh From: jc_pugh@FCCC.EDU ("Dr. John Pugh") Newsgroups: bionet.molbio.rapd Subject: None Date: 30 Oct 1995 14:01:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe ------------------------------------------------------------------------------- Dr. John Pugh at : jc_pugh@fccc.edu The Fox Chase Cancer Center 7701 Burholme Avenue Philadelphia, PA 19111 (215) 728-4780 From owner-rapd@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!ACD.TUSK.EDU!Prakash From: Prakash@ACD.TUSK.EDU (C. S. Prakash) Newsgroups: bionet.molbio.rapd Subject: Re: primer kits for RAPDs Date: 30 Oct 1995 13:40:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HX1V6HFRS20006YJ@Acd.Tusk.Edu> NNTP-Posting-Host: net.bio.net University of British Columbia sells many RAPD primer kits. Contact them at hobbs@unixg.ubc.ca (John Hobbs) Operon Inc. also sells RAPD kits. >Does anyone know who produces RAPD primer kits >I have used the Advanced Biotechnology kits and need >some more. >I am interested in some kits which are cheap as well as effective >for mammalian RAPD production!! > >many thanks C. S. Prakash Voice (334) 727 - 8023 Tuskegee University Fax (334) 727 - 8552 School of Agriculture Alt. Fax (334) 727 - 8493 Tuskegee, Alabama 36088 USA Email: Prakash@Acd.Tusk.Edu From owner-rapd@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!daresbury!not-for-mail From: J J Dooley Newsgroups: bionet.molbio.rapd Subject: primer kits for RAPDs Date: 30 Oct 1995 14:59:41 -0000 Lines: 7 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <472p8t$7df@mserv1.dl.ac.uk> Original-To: rapd@dl.ac.uk Does anyone know who produces RAPD primer kits I have used the Advanced Biotechnology kits and need some more. I am interested in some kits which are cheap as well as effective for mammalian RAPD production!! many thanks From owner-rapd@net.bio.net Sun Oct 29 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!in1.uu.net!uunet.ca!news.uunet.ca!banshee.uunet.ca!nobody Message-ID: <951029.133804.16558@banshee.uunet.ca> Sender: nobody@banshee.uunet.ca X-Nntp-Posting-Host: 142.77.242.63 From: Gwade@physiology.uwo.ca Newsgroups: bionet.molbio.rapd Subject: Lightening FAST ELISA & DNA DOT BLOT Date: Sun, 29 Oct 1995 23:04:30 GMT X-Newsreader: Forte Free Agent 1.0.82 Lines: 24 Kalyx Biosciences (Ottawa, Ontario, Canada) has developed a new matrix on which ELISA and Nucleic Acid Hybridization can be performed. EIA results can be acheived in minutes while DNA dot blot experiments can be performed in hours as opposed to days. Polymacron is less expensive than nylon or other matrices. We've used this stuff in the lab and the results are incredible. I've also had great help from the technical support people at Kalyx. Give em' a call or email them to get product literature. Kalyx@magi.com 800 661-6052 Greg Wade Ph.D. Candidate Department of Physiology The University of Western Ontario. London, Ontario Canada Gwade@physiology.uwo.ca From owner-rapd@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: Brian B. Jiang <73344.437@CompuServe.COM> Newsgroups: bionet.molbio.proteins,bionet.molbio.rapd,bionet.molbio.yeast,bionet.molec-model,bionet.mycology Subject: Anti-Immigration Law Status/Seminar Date: 31 Oct 1995 08:43:31 GMT Organization: The Law Office of Brian B. Jiang Lines: 105 Message-ID: <474njj$58u$1@mhafn.production.compuserve.com> Xref: biosci bionet.molbio.proteins:6142 bionet.molbio.rapd:1356 bionet.molbio.yeast:4082 bionet.molec-model:642 bionet.mycology:3017 RE: New Development On the Anti-Immigration Bill(HR 2202) and Upcoming Seminar Hi folks: As of today, the House Judiciary Committee has completed its markup on the Lamar Bill after 9 days of debate over several weeks. Despite all the bad news, such as reduction of legal immigration and elimination of some family categories, there are a few signs of relief. Both outstanding researchers/professors and national interest waivers were restored to the Bill. So professionals( scientists, post-docs, engineers etc.) can still apply for permanent residence without labor certification and sponsorship of their employers. On the other hand, for those who have to apply through labor cert, they are, as usual, still at the mercy of the Labor Dept. Any positive change is yet to be seen. Although waiver of labor certification and employer sponsorship is still obtainable for those who qualify, difficulties have been on the rise and more are expected. Nearly all four Service Centers have implemented some newly proposed requirements that make it very difficult to obtain an approval. According to administrative rules no law should be carried out until it is final. Despite the rules, INS is already treating the proposals like enforceable law. In the real world, things are not always the way they are supposed to be. This is even more so in immigration practice with today's anti-immigration fervor. I am an immigration attorney specializing in employment based immigration(1st and 2nd preference). I will hold seminars on how to apply for a green card without labor certification and sponsorship of the employer. The intended audiences of my seminar are professionals with advanced degrees or a bachelor degree plus five years of work experience. I will also cover the impact of HR 2202 on future immigration. The locations of the seminar will include: 1. University of Florida at Gainesville University Center Hotel (President's Ballroom) 1535 SW Archer Road Gainesville, FL Tel: (904)371-3333 Time: 11/12/95 Sunday 2:00-4:00pm 2. Columbia University at New York City Columbia University 517 Hamilton Hall (close to W. 116th St. & Broadway) Time: 11/13/95 Monday 7:30-9:30pm 3. Princeton University Location to be decided Time 11/14/95 Tuesday 4. Baltimore near University of Maryland Holiday Inn Baltimore Inner Harbor (Chesateake Ballroom #1) 301 W. Lombard Street Baltimore, MD Tel: (410)685-3500 Time: 11/15/95 Wed. 7:30-9:30pm 5. Pittsburgh near Univ. of Pittsburgh Pittsburgh Green Tree Marriott (Near University of Pittsburgh) 101 Marriott Drive Pittsburgh, PA Tel: (412)922-8400 Time: 11/16/95 Thursday 7:30-9:30pm 6. Boston at Cambridge Hyatt Regency Cambridge 575 Memorial Drive Cambridge, MA Tel: (617)492-1234 Time 11/17/95 Friday 7:30-9:30pm 7. University of Connecticut Location to be decided Time 11/18/95 Saturday 2:30-4:30pm 8. Palo Alto near Stanford University Holiday Inn Palo Alto (Near Stanford University) 625 El Camino Real Palo Alto, CA Tel: (415)328-2800 Time 11/19/95 Sunday 7:30-9:30pm I will conduct these seminars during these time. All questions, comment, and suggestions are welcome. You can also reach me at tel. (619)278-5480 or (619)278-5492. Brian B. Jiang Esq. Email: 73344.437@compuserve.com From owner-rapd@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.tamu.edu!486MULLET2 From: PULANCH@bioch.tamu.edu (Paul E. Ulanch) Newsgroups: bionet.molbio.rapd Subject: Re: bulk segregant analysis Date: Tue, 31 Oct 95 18:28:20 GMT Organization: Texas A&M University Lines: 32 Message-ID: <475ps4$s0j@news.tamu.edu> References: NNTP-Posting-Host: 486mullet2.tamu.edu X-Newsreader: News Xpress Version 1.0 Beta #3 In article , t80bad1@CORN.CSO.NIU.EDU (Brad A Didion) wrote: >We are considering using BSA to compare band phenotypes between two >groups of animals with respect to an abnormality. The animals differ only >for the trait as the genetic backgrounds are the same (i.e. within line). >How many template samples are to be represented per bulk? just curious. > >Brad > We used BSA to analyze a Sorghum population that segregated for only one trait and included 8 F2 progeny per bulk. You can add more or less it all depends on you. The more progeny you add to the bulks, the greater chance you will have of losing markers that are linked to your favorite gene. The less progeny, the greater number of markers you will have. I would start with eight and see what RAPDs you pull out. If you need more, then make smaller bulks, if you need to week out potential RAPDs because you get too many, make your bulk population larger. Good luck, Paul "Goose" Ulanch Dept. Biochem/Biophys Texas A&M University College Station, TX 77843-2128 (409) 847-9379 PULANCH@bioch.tamu.edu From owner-rapd@net.bio.net Mon Oct 30 22:00:00 1995 Path: biosci!CORN.CSO.NIU.EDU!t80bad1 From: t80bad1@CORN.CSO.NIU.EDU (Brad A Didion) Newsgroups: bionet.molbio.rapd Subject: bulk segregant analysis Date: 30 Oct 1995 09:36:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: We are considering using BSA to compare band phenotypes between two groups of animals with respect to an abnormality. The animals differ only for the trait as the genetic backgrounds are the same (i.e. within line). How many template samples are to be represented per bulk? just curious. Brad