From owner-rapd@net.bio.net Wed Nov 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!sunsite.doc.ic.ac.uk!rfhsun3.rfhsm.ac.uk!rfhsm.ac.uk!k9mhc From: k9mhc@rfhsm.ac.uk (vid mohan-ram) Newsgroups: bionet.molbio.rapd Subject: Re: primer kits for RAPDs Date: Thu, 2 Nov 1995 09:54:47 +1000 Organization: Royal Free Hospital School of Medicine Lines: 6 Distribution: bionet Message-ID: References: <472p8t$7df@mserv1.dl.ac.uk> NNTP-Posting-Host: porcupine.rfhsm.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] operon technologies do a whole set of kits of which i've used two (20 rapd primers per kit)....they're 'agents' over here are vh-bio (0191 492 0022) not connected with the company From owner-rapd@net.bio.net Wed Nov 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!pendragon.jsc.nasa.gov!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!rfhsun3.rfhsm.ac.uk!rfhsm.ac.uk!k9mhc From: k9mhc@rfhsm.ac.uk (vid mohan-ram) Newsgroups: bionet.molbio.rapd Subject: statistical analysis Date: Thu, 2 Nov 1995 10:02:09 +1000 Organization: Royal Free Hospital School of Medicine Lines: 8 Distribution: world Message-ID: NNTP-Posting-Host: porcupine.rfhsm.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] hi, want to know what's the program of choice when it comes to analyzing rapd products?...and how to download/get it...?.....thanks vid From owner-rapd@net.bio.net Wed Nov 01 22:00:00 1995 Path: biosci!bcm.tmc.edu!pendragon.jsc.nasa.gov!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!rfhsun3.rfhsm.ac.uk!rfhsm.ac.uk!k9mhc From: k9mhc@rfhsm.ac.uk (vid mohan-ram) Newsgroups: bionet.molbio.rapd Subject: rapd artifactual bands Date: Thu, 2 Nov 1995 10:00:01 +1000 Organization: Royal Free Hospital School of Medicine Lines: 10 Distribution: world Message-ID: NNTP-Posting-Host: porcupine.rfhsm.ac.uk X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] ok...here we go....have any of you who have used rapd primers and done a no template control (to check for contamination) get what i do? for almost all primers i've used i get STUNNING amplification products ranging from 200bp up to 2.5kb with single RAPD primer and no dna at all....consulting the refs......this is 'normal'...however it is a little offputting that the control rxns produce so many artifactual bands.....anyone out there shed some light as to how a single 10mer primer can cause so much secondary structures so as to create these large products?.........thanks for any advice From owner-rapd@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!pendragon.jsc.nasa.gov!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!simtel!zombie.ncsc.mil!cs.umd.edu!haven.umd.edu!purdue!lerc.nasa.gov!magnus.acs.ohio-state.edu!dsammata From: dsammata@magnus.acs.ohio-state.edu (Diana Sammataro) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.rapd Subject: RAPDs ups and downs Date: 3 Nov 1995 15:19:33 GMT Organization: The Ohio State University Lines: 20 Message-ID: <47dbu5$fs@charm.magnus.acs.ohio-state.edu> NNTP-Posting-Host: beauty.magnus.acs.ohio-state.edu Xref: biosci bionet.molbio.methds-reagnts:35875 bionet.molbio.rapd:1363 After working with RAPDs and mites (which have so little DNA I cannot quantify) I have finally found a few interesting items. 1. I have now three protocols that work for bees, blood and mites, if anyone is interested, contact me. 2. I have found that spurious banding patterns will go away if the annealing temperatures are raised from 37 to 40 or higher. 3. I am still having trouble extracting DNA from these mites. Can anyone give me suggestions? I have tried the Phenol/chloroform, salt, and KoAC for Drosophila. All work except the former. Bee DNA cleans up nicely with dialysis but I loose too much mite DNA. Thanks in adavance Diana Sammataro, Ph.D. (now) Dept. Entomology Ohio State Univeristy 1735 Neil Ave Columbus, OH 43210 1220 dsammata@magnus.acs.ohio-state.edu From owner-rapd@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!ASRR.ARSUSDA.GOV!abartlet From: abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") Newsgroups: bionet.molbio.rapd Subject: Re: rapd artifactual bands Date: 3 Nov 1995 13:41:50 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net Your results do not seem 'normal' to me. We run two 'no template' reactions on each gel and almost never get bands in those lanes. If we do get bands in the control lanes we conclude that we have somehow contaminated our reaction mixture and toss the whole gel out and redo the reactions. I have been told that a low molecular weight band (<200 bp) we get with some primers (the band appears on every lane regardless of the template source) is due to the polyprimer association you speak of, but there is never more than one band even in blank lanes. Alan C. Bartlett "GENES-R-US" "ALTERATIONS AVAILABLE!^" "^some restrictions may apply:)" From owner-rapd@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!ACD1.BYU.EDU!FARMERJ From: FARMERJ@ACD1.BYU.EDU ("James L. Farmer") Newsgroups: bionet.molbio.rapd Subject: Re: rapd artifactual bands Date: 3 Nov 1995 08:42:40 -0800 Organization: Brigham Young University Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <2EF57A32CA@acd1.byu.edu> NNTP-Posting-Host: net.bio.net > > To: rapd@net.bio.net > > From: k9mhc@rfhsm.ac.uk (vid mohan-ram) > > Subject: rapd artifactual bands > > Date: Thu, 2 Nov 1995 10:00:01 +1000 > > > ok...here we go....have any of you who have used rapd primers and done a no > template control (to check for contamination) get what i do? > for almost all primers i've used i get STUNNING amplification products ranging > from 200bp up to 2.5kb with single RAPD primer and no dna at all....consulting > the refs......this is 'normal'...however it is a little offputting that the > control rxns produce so many artifactual bands.....anyone out there shed some > light as to how a single 10mer primer can cause so much secondary structures > so as to create these large products?.........thanks for any advice > > I used to get these too, but in the last year I have pretty much eliminated them. I don't know how, but I use University of British Columbia RAPD primers, P-E Amplitaq, P-E buffer II, P-E MgCl2, and ProMega dNTP's. The DNA is prepared using buffers I mix myself using HPLC-grade water. I use Boehringer-Mannheim Proteinase K. I have recently tried the P-E Stoffel fragment and I get terrible results with my DNA (crude extract of Drosophila pseudoobscura). Also, the Stoffel fragment DOES give me lots of bands in a tube with no template. Other people here use Stoffel fragment with good results, although they tell me that it seems to preferentially amplify smaller fragments. I am going to stick with Amplitaq for my current project. James Farmer Dept. of Zoology, 571 WIDB Brigham Young University Provo, Utah 84604, USA FARMERJ@BYU.EDU 801-378-2153 (voice) 801-378-7423 (FAX) From owner-rapd@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!newsspool.doit.wisc.edu!news.doit.wisc.edu!news From: markb@ravel.zoology.wisc.edu (Mark E. Berres) Newsgroups: bionet.molbio.rapd Subject: Re: primer kits for RAPDs Date: 3 Nov 1995 15:05:53 GMT Organization: The University of Wisconsin, Madison Lines: 37 Distribution: bionet Message-ID: <47db4h$rl8@news.doit.wisc.edu> References: Reply-To: markb@ravel.zoology.wisc.edu NNTP-Posting-Host: ravel.zoology.wisc.edu In article , k9mhc@rfhsm.ac.uk (vid mohan-ram) writes: > operon technologies do a whole set of kits of which i've used two (20 rapd > primers per kit)....they're 'agents' over here are vh-bio (0191 492 0022) Also check out Genosys. They have quite a few arbitrary 10-mer kits available. In addition, a new product has recently become available called the decamerarray. It is a library of 1,040 10-mers with G+C contents ranging from 50 to 80 percent. Check it out yourself on their (excellent) web page: http://www.genosys.com/ Mark -- Mark E. Berres Birge Hall Office, Room 441 Department of Zoology 430 Lincoln Drive Department of Genetics Madison, WI. 53706-1313 University of Wisconsin at Madison Ph: (608)-265-6427 -----BEGIN PGP PUBLIC KEY BLOCK----- Version: 2.6.2 mQCNAy9eAb0AAAEEALiWUAWSEV4g9Glu0Al8VxA62PBkfeSZJC/90guCvZtwgDvC fLDA862kj9Bs5Ae4KwsbYag6QSkG1JqfHzufAFLgTgkt5k68TLfEAsut/WMy8ix3 wVoQAZTbQnOTeGmG2ceA1cYY9pDuzrUAt7vhpBc9pp4rEHXX2Uljqit9IVslAAUR tC1NYXJrIEUuIEJlcnJlcyA8bWFya2JAcmF2ZWwuem9vbG9neS53aXNjLmVkdT4= =IUu8 -----END PGP PUBLIC KEY BLOCK----- From owner-rapd@net.bio.net Thu Nov 02 22:00:00 1995 Path: biosci!RAVEL.ZOOLOGY.WISC.EDU!markb From: markb@RAVEL.ZOOLOGY.WISC.EDU ("Mark E. Berres") Newsgroups: bionet.molbio.rapd Subject: Re: primer kits for RAPDs Date: 3 Nov 1995 08:29:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511031608.KAA08724@ravel.zoology.wisc.edu> NNTP-Posting-Host: net.bio.net According to C. S. Prakash: > > University of British Columbia sells many RAPD primer kits. > > Contact them at hobbs@unixg.ubc.ca (John Hobbs) > > Operon Inc. also sells RAPD kits. > > > > >Does anyone know who produces RAPD primer kits > >I have used the Advanced Biotechnology kits and need > >some more. > >I am interested in some kits which are cheap as well as effective > >for mammalian RAPD production!! > > > >many thanks > Also check out Genosys. They have quite a few arbitrary 10-mer kits available. In addition, a new product has recently become available called the decamerarray. It is a library of 1,040 10-mers with G+C contents ranging from 50 to 80 percent. Check it out yourself on their (excellent) web page: http://www.genosys.com/ Mark -- Mark E. Berres Birge Hall Office, Room 441 Department of Genetics 430 Lincoln Drive Department of Zoology Madison, WI. 53706-1313 University of Wisconsin at Madison Ph: (608)-265-6427 -----BEGIN PGP PUBLIC KEY BLOCK----- Version: 2.6.2 mQCNAy9eAb0AAAEEALiWUAWSEV4g9Glu0Al8VxA62PBkfeSZJC/90guCvZtwgDvC fLDA862kj9Bs5Ae4KwsbYag6QSkG1JqfHzufAFLgTgkt5k68TLfEAsut/WMy8ix3 wVoQAZTbQnOTeGmG2ceA1cYY9pDuzrUAt7vhpBc9pp4rEHXX2Uljqit9IVslAAUR tC1NYXJrIEUuIEJlcnJlcyA8bWFya2JAcmF2ZWwuem9vbG9neS53aXNjLmVkdT4= =IUu8 -----END PGP PUBLIC KEY BLOCK----- From owner-rapd@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.molbio.rapd Subject: Two Assistant Professor Positions Date: 6 Nov 1995 06:42:28 -0800 Organization: University of Arkansas Lines: 70 Sender: daemon@net.bio.net Distribution: world Message-ID: <202A565E7E@mercury.uark.edu> Official statement: TWO ASSISTANT PROFESSORS The Department of Biological Sciences of the University of Arkansas, Fayetteville is searching for a Plant Physiologist and a Plant Geneticist for tenure-track appointments beginning 15 August 1996. Preference for the Geneticist position will be given to candidates using molecular approaches in the areas of Developmental or Population Genetics. Candidates must have a Ph.D. and post-doctoral experience. Appointees must be prepared to participate in freshman, upper, and graduate level courses, and to establish an independent research program which incorporates Master's and Ph.D. students. Salary and start-up are competitive. Review of applicants begins December 1, 1995 and continues until the positions are filled. Submit letter of application, curriculum vitae, statements of research and teaching interest, reprints, and have three letters of recommendation sent to Dr. Richard L. Meyer, Chair, Plant Physiologist Search Committee, OR Dr. Edwin B. Smith, Chair, Plant Geneticist Search Committee, Department of Biological Sciences, SCEN-629, University of Arkansas, Fayetteville, AR 72701. The University of Arkansas is an Equal Opportunity/Affirmative Action Institution -- women and minorities are encouraged to apply. The non-official two-cents worth: The Department of Biological Sciences is a diverse department comprising 26 faculty in disciplines ranging from ecology to molecular. We award Bachelor's (BA and BS) in Biology, Microbiology, Botany, and Zoology (number of majors currently around 200). We also have approximately 60 graduate students. The campus is located in Fayetteville in the hills of Northwest Arkansas. The University has colleges in Arts & Sciences, Engineering, Business, Law, and Agriculture. Student population is around 15,000. You can find out more by browsing at: http://www.uark.edu or if you only want to find out about the Department and its associated Wildlife Cooperative Research Unit you can point your Web viewer at: http://comp.uark.edu/~bioinfo/bisc.html If you have further questions you may contact either of us directly or any of the other faculty listed on our Web Page. //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| |drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// or ---------------------------------------- Dave Evans Department of Biological Sciences University of Arkansas Fayetteville, AR 72701 PH: 501-575-7093 From owner-rapd@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!ACD1.BYU.EDU!FARMERJ From: FARMERJ@ACD1.BYU.EDU ("James L. Farmer") Newsgroups: bionet.molbio.rapd Subject: responses to commercial messages Date: 6 Nov 1995 09:14:59 -0800 Organization: Brigham Young University Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <777E2F79A6@acd1.byu.edu> Please do not send responses to commercial messages back to rapd. If you would like to vent your frustrations, may I suggest that you do what I do? Forward the complete message, with your comments, to the postmaster of the offending person. For instance, if the commercial message came from SOMEPERSON@GREEDY.COM, forward the message back to POSTMASTER@GREEDY.COM. If a postmaster gets enough complaints, that person may take steps against the offending party. At least, it gives me a little satisfaction to try. James Farmer Dept. of Zoology, 571 WIDB Brigham Young University Provo, Utah 84604, USA FARMERJ@BYU.EDU 801-378-2153 (voice) 801-378-7423 (FAX) From owner-rapd@net.bio.net Sun Nov 05 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.sprintlink.net!redstone.interpath.net!apollo.nortel.net!NewsWatcher!user From: drdad@ktc.com Newsgroups: bionet.molbio.rapd Subject: Re: rapd artifactual bands Date: 6 Nov 1995 21:54:15 GMT Organization: A poorly-installed InterNetNews site Lines: 32 Distribution: world Message-ID: References: <2EF57A32CA@acd1.byu.edu> NNTP-Posting-Host: norteld253.nortel.net In article <2EF57A32CA@acd1.byu.edu>, FARMERJ@ACD1.BYU.EDU ("James L. Farmer") wrote: > > > To: rapd@net.bio.net > > > From: k9mhc@rfhsm.ac.uk (vid mohan-ram) > > > Subject: rapd artifactual bands > > > Date: Thu, 2 Nov 1995 10:00:01 +1000 > > > > > > > ok...here we go....have any of you who have used rapd primers and done a no > > template control (to check for contamination) get what i do? > > for almost all primers i've used i get STUNNING amplification products ranging > > from 200bp up to 2.5kb with single RAPD primer and no dna at all....consulting > > the refs......this is 'normal'...however it is a little offputting that the > > control rxns produce so many artifactual bands.....anyone out there shed some > > light as to how a single 10mer primer can cause so much secondary structures > > so as to create these large products?.........thanks for any advice Hi there, I will get those bands you describe, depending on the RAPD primer. I have cleared almost all of the "no template" bands by using the Taqstart antibody from Clontech. I have no ties to Clontech. Good Luck, From owner-rapd@net.bio.net Mon Nov 06 22:00:00 1995 Path: biosci!ACD1.BYU.EDU!FARMERJ From: FARMERJ@ACD1.BYU.EDU ("James L. Farmer") Newsgroups: bionet.molbio.rapd Subject: (Fwd) The 'spotter' Spam Date: 7 Nov 1995 09:40:01 -0800 Organization: Brigham Young University Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <8FE7753CE3@acd1.byu.edu> NNTP-Posting-Host: net.bio.net I recently posted a suggestion about how to handle commercial messages which are posted to bionet. I was very encouraged this morning to receive the following message concerning a complaint I made about a recent posting to this user group. ------- Forwarded Message Follows ------- Date: Mon, 6 Nov 1995 14:50:07 -0800 From: support@netcom.com (Netcom Support) To: "James, , Farmer"@office4.netcom.com, L.@office4.netcom.com Subject: The 'spotter' Spam This is an automatically generated message in response to your report of abuse by spotter@netcom.com. Due to the volume of mail received regarding this user, we are unable to answer each of your reports individually. Please be aware that if you sent more than one report, you will only receive this message once. We have killed this user's processes and suspended the account. Many of the posts made from this account have been cancelled and we are currently locating and cancelling any other posts by this user. Regards, Linnea ------------------------------------------------------------------------------ Linnea Abuse Coordinator abuse@netcom.com Netcom On-Line Communications Services James Farmer Dept. of Zoology, 571 WIDB Brigham Young University Provo, Utah 84604, USA FARMERJ@BYU.EDU 801-378-2153 (voice) 801-378-7423 (FAX) From owner-rapd@net.bio.net Tue Nov 07 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!Germany.EU.net!ieunet!news.tcd.ie!bioftp.unibas.ch!citi2.fr!jussieu.fr!jouy.inra.fr!usenet From: Yves Bertheau Newsgroups: bionet.molbio.rapd Subject: Re: rapd artifactual bands Date: 8 Nov 1995 17:44:09 GMT Organization: Institut National de la Recherche Agronomique Lines: 32 Message-ID: <47qq99$bfj@saphir.jouy.inra.fr> References: NNTP-Posting-Host: solair.inapg.inra.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; SunOS 5.3 sun4c) X-URL: news:Pine.SOL.3.91.951103162835.9726A-100000@asrr.arsusda.gov abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") wrote: >Your results do not seem 'normal' to me. We run two 'no template' >reactions on each gel and almost never get bands in those lanes. If we >do get bands in the control lanes we conclude that we have somehow >contaminated our reaction mixture and toss the whole gel out and redo the >reactions. I have been told that a low molecular weight band (<200 bp) >we get with some primers (the band appears on every lane regardless of >the template source) is due to the polyprimer association you speak of, >but there is never more than one band even in blank lanes. > >Alan C. Bartlett >"GENES-R-US" >"ALTERATIONS AVAILABLE!^" >"^some restrictions may apply:)" > > I agree... It is not 'normal' to get contaminations in the amplification controls !!! We use RAPD only to check the 'PCR quality' of DNA to be amplified for the detection by PCR of bacterial pathogens but our negative control (i.e. without added DNA) should be always(and are) without contaminating bands... Yves Bertheau INRA ,INA P-G Pathologie Vegetale 16 rue Claude Bernard 75231 Parix cedex 05 Paris France From owner-rapd@net.bio.net Tue Nov 07 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!EU.net!ieunet!news.tcd.ie!bioftp.unibas.ch!news.vub.ac.be!ben.vub.ac.be!wmoens From: William Moens Newsgroups: bionet.molbio.rapd Subject: Re: rapd artifactual bands Date: Wed, 8 Nov 1995 15:28:28 +0000 Organization: Brussels Free Universities VUB/ULB Lines: 23 Message-ID: References: NNTP-Posting-Host: ben.vub.ac.be. Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: Hi! Have a look to Hughes' paper in J.Clin.Microbiology,Aug 1994, Vol32, p2007-2008: a good lesson about trusting Companies "Quality Insurance"... W.Moens SBB-IHE Brussels Belgium On Thu, 2 Nov 1995, vid mohan-ram wrote: > > > ok...here we go....have any of you who have used rapd primers and done a no > template control (to check for contamination) get what i do? > for almost all primers i've used i get STUNNING amplification products ranging > from 200bp up to 2.5kb with single RAPD primer and no dna at all....consulting > the refs......this is 'normal'...however it is a little offputting that the > control rxns produce so many artifactual bands.....anyone out there shed some > light as to how a single 10mer primer can cause so much secondary structures > so as to create these large products?.........thanks for any advice > > From owner-rapd@net.bio.net Wed Nov 08 22:00:00 1995 Path: biosci!UNIXG.UBC.CA!hobbs From: hobbs@UNIXG.UBC.CA (John Hobbs) Newsgroups: bionet.molbio.rapd Subject: Postdoctoral fellow position. Date: 9 Nov 1995 09:53:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511091751.JAA03212@unixg.ubc.ca> NNTP-Posting-Host: net.bio.net Postdoctoral fellow: Microbial Diversity in Soils Dr. Julian Davies is seeking a postdoctoral fellow (or similar level scientist) to work on the development of methods for DNA extraction and analysis (RAPD, AFLP, etc.) as part of a project studying microbial diversity in forest soils. This is a joint project with Dr. Paige Axelrood, Forest Biotechnology, B.C. Research Institute, and the West-East Centre, and is funded by the Forest Renewal Programme. Interested parties seeking further details should contact: Dr. Julian E. Davies Professor and Head Dept. of Microbiology and Immunology University of British Columbia Wesbrook Building, 6174 University Boulevard Vancouver, B.C. V6T 1Z3 CANADA (E-mail to jed@unixg.ubc.ca) Dr. John Hobbs Nucleic Acid - Protein Service (NAPS) Unit Biotechnology Laboratory Room 237, Wesbrook Building 6174 University Boulevard University of British Columbia Vancouver, V6T 1Z3 Canada. FAX (604)822-5437 or (604)822-0676; Tel. (604)822-6373 From owner-rapd@net.bio.net Thu Nov 09 22:00:00 1995 Path: biosci!PIRA.CENA.USP.BR!DAVHMOON From: DAVHMOON@PIRA.CENA.USP.BR Newsgroups: bionet.molbio.rapd Subject: Addresses Date: 10 Nov 1995 05:10:47 -0800 Organization: CENA/USP Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <1DF1D3300FD@pira.cena.usp.br> NNTP-Posting-Host: net.bio.net Dear netters, Please be patient with me, I know this is not strictly a RAPD problem but I have tried other means without much success. I would like to know if anyone out there has the E-mail addresses of the following scientists:- Chris Lamb, the Salk Institute, La Jolla, CA Andrew Staehelin or Felix Mauch, University of Colorado, Boulder. John A. Ryals, CIBA-GEIGY Corporation, North Carolina. I hope there will not will be not too many complaints about misuse of the network, at a time when there is obviously a large number of people abusing the system. Thankyou in advance for any help that you can give, yours sincerely David From owner-rapd@net.bio.net Tue Nov 14 22:00:00 1995 Path: biosci!MERCURY.UARK.EDU!DRHOADS From: DRHOADS@MERCURY.UARK.EDU ("Douglas Rhoads") Newsgroups: bionet.molbio.rapd Subject: Two Assistant Professor Positions Date: 15 Nov 1995 13:01:52 -0800 Organization: University of Arkansas Lines: 71 Sender: daemon@net.bio.net Distribution: world Message-ID: <4E9F0D53E9@mercury.uark.edu> NNTP-Posting-Host: net.bio.net Official statement: TWO ASSISTANT PROFESSORS The Department of Biological Sciences of the University of Arkansas, Fayetteville is searching for a Plant Physiologist and a Plant Geneticist for tenure-track appointments beginning 15 August 1996. Preference for the Geneticist position will be given to candidates using molecular approaches in the areas of Developmental or Population Genetics. Candidates must have a Ph.D. and post-doctoral experience. Appointees must be prepared to participate in freshman, upper, and graduate level courses, and to establish an independent research program which incorporates Master's and Ph.D. students. Salary and start-up are competitive. Review of applicants begins December 1, 1995 and continues until the positions are filled. Submit letter of application, curriculum vitae, statements of research and teaching interest, reprints, and have three letters of recommendation sent to Dr. Richard L. Meyer, Chair, Plant Physiologist Search Committee, OR Dr. Edwin B. Smith, Chair, Plant Geneticist Search Committee, Department of Biological Sciences, SCEN-629, University of Arkansas, Fayetteville, AR 72701. The University of Arkansas is an Equal Opportunity/Affirmative Action Institution -- women and minorities are encouraged to apply. The non-official two-cents worth: The Department of Biological Sciences is a diverse department comprising 26 faculty in disciplines ranging from ecology to molecular. We award Bachelor's (BA and BS) in Biology, Microbiology, Botany, and Zoology (number of majors currently around 200). We also have approximately 60 graduate students. The campus is located in Fayetteville in the hills of Northwest Arkansas. The University has colleges in Arts & Sciences, Engineering, Business, Law, and Agriculture. Student population is around 15,000. You can find out more by browsing at: http://www.uark.edu or if you only want to find out about the Department and its associated Wildlife Cooperative Research Unit you can point your Web viewer at: http://comp.uark.edu/~bioinfo/bisc.html If you have further questions you may contact either of us directly or any of the other faculty listed on our Web Page. //========================================================\\ ||Doug Rhoads || Dept. of Biological Sciences|| |drhoads@mercury.uark.edu || 601 Science Engineering || ||drhoads@uafsysb.uark.edu || University of Arkansas || ||501-575-3251 || Fayetteville, AR 72701 || ==========================================================|| || My Dogma Just Got Run Over by Someone's Karma || \\========================================================// or ---------------------------------------- Dave Evans Department of Biological Sciences University of Arkansas Fayetteville, AR 72701 PH: 501-575-7093 From owner-rapd@net.bio.net Tue Nov 14 22:00:00 1995 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!enews.sgi.com!sgigate.sgi.com!swrinde!newsfeed.internetmci.com!psgrain!charnel.ecst.csuchico.edu!steroid.ecst.csuchico.edu!sleonard From: "Sarah E. Leonard" Newsgroups: bionet.molbio.rapd Subject: Myxomycetes (Slime Moulds) Date: Tue, 14 Nov 1995 16:18:17 -0800 Organization: California State University, Chico Lines: 15 Message-ID: NNTP-Posting-Host: steroid.ecst.csuchico.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII I am looking for a protocol for PCR methods on Myxomycetes. I need some DNA extraction techniques and primers that will work on the Myxomycetes. If you can be of any assistance, please let me know. Thank you in advance. Smile, Sarah E. Leonard sleonard@ecst.csuchico.edu Dept. of Biology 916.898.5356 CSU, Chico Chico, CA 95929-0515 From owner-rapd@net.bio.net Thu Nov 16 22:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: IMPORTANT: BIOSCI miniFAQ Date: 17 Nov 1995 02:00:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 196 Sender: daemon@net.bio.net Distribution: world Message-ID: <199511171000.CAA12802@net.bio.net> NNTP-Posting-Host: net.bio.net This is a new "miniFAQ" designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) What to do about "spams," i.e., junk mail, ads, etc. 2) Examples of subscribing and unsubscribing to the mailing lists. 3) How to access BIOSCI/bionet newsgroup archives. 4) The BIOSCI user address and research interest directory. 1) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups) and mailing lists. The same postings are distributed on both media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the newsgroups from about 95% of the spams that are being sent to date. This means that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings. Unfortunately there are easy ways for determined spammers to override the moderation mechanism. We are working on new systems to provide access to our newsgroups over the WWW. These should be available soon, probably November 1995, and will allow you to use your Web browser to look at the news postings. While this will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. 2) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 3) How to access BIOSCI/bionet newsgroup archives. -------------------------------------------------- Back postings of all BIOSCI/bionet newsgroups can be found on the World Wide Web at URL http://www.bio.net/. There are several searchable newsgroup indices at this site. E-mail users can search the BIOSCI archives by using our waismail e-mail server. For instructions send the message help to waismail@net.bio.net. Leave the Subject: line blank (anything entered on the Subject: line is ignored). 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-rapd@net.bio.net Sun Nov 19 22:00:00 1995 Path: biosci!nbri.sirnetd.ernet.in!sar From: sar@nbri.sirnetd.ernet.in ("Shirish A. Ranade") Newsgroups: bionet.molbio.rapd Subject: Gel Documentation and Analysis system Date: 19 Nov 1995 21:12:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi Netters! I am contemplating a purchase of Gel Documentation and Analysis system for use in the RAPD analysis programme that we have here. I would appreciate your views and experiences regarding these systems. If you have any, please mail back to me on my personal address. To start with I have shortlisted the following : 1. Stratagene make Eagle Eye II still video system priced at around DM 40000, including software and Customized computer 2. Biorad make Gel Doc 1000 Fluorescent Gel System priced at around $21000, including software but does not include a Computer. 3. UVP Gel Documentation system priced at Pound Sterling 8000 without computer and software. I prefer IBM-PC and compatible hardwares running on DOS 6.22 or higher and/or Windows. What, if any, specific requirements have to be satisfied by such systems especially with reference to the CCD Camera and Frame-Grabber cards? Has anyone used more than 1 type of system and if so which of them was the better one? Can data generated by the system be read by a RAPD or RFLP analysis software which in turn can permit several statistical analyses such as distance estimates, cluster analyses etc.? Thanking you all in advance, Sincerely, Shirish Ranade PS. The response from the Commercial Sources and Manufacturers may please be sent directly to me at the addresses given below, and not to the network please. Thanks. >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> DR. SHIRISH A. RANADE SNAIL MAIL : CENTRE FOR PLANT MOLECULAR BIOLOGY NATIONAL BOTANICAL RESEARCH INSTITUTE RANA PRATAP MARG, LUCKNOW 226001. (U.P.) INDIA. FAX NOS. : (91) 522 282881 OR 282849 E-MAIL (PERSONAL) : SAR@NBRI.SIRNETD.ERNET.IN E-MAIL (PUBLIC) : MANAGER@NBRI.SIRNETD.ERNET.IN <<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< From owner-rapd@net.bio.net Thu Nov 23 22:00:00 1995 Path: biosci!UNIXG.UBC.CA!jcarlson From: jcarlson@UNIXG.UBC.CA ("Dr. John Carlson") Newsgroups: bionet.molbio.rapd Subject: Re: rapd artifactual bands Date: 24 Nov 1995 10:07:31 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 51 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <24NOV95.12764213.0359.MUSIC@MUSICB.MCGILL.CA> NNTP-Posting-Host: net.bio.net Nadia, et. el.: We have observed non-primer dimer bands in no-template DNA controls with certain of our UBC primers too. Many primers do not give artifactual bands, however. The problem lies with DNA contamination in the DNA polymerase of bacterial origin. The amount of contaminating DNA will vary between suppliers and lots, but will always be present to a certain extent. Of course contaminating DNA in trace amounts is impossible to avoid in the Taq purification process (regardless of supplier's claims), and contaminating DNA is easier to amplify and thus detect by the RAPD process than by standard PCR. The observation that only certain primers will amplify the contaminating bacterial DNA, presumably derives from sequence homology. No amount of purification of the primers or buffers will help in this case. On the other hand, when you use about a nanogram of template DNA, the small amount of contaminating DNA in the Taq will be swamped by your template and will not compete effectively for primer and thus should not affect your results. It never affects our results when we use commercially available Taq anyways (i.e. we do not see the two or three bands in the control lane in our amplified experimental samples). Cheers. John Carlson, UBC On 24 Nov 1995, CAMPEOL,NADIA,MS wrote: > November 24, 1995 > > Well I am releaved that people using RAPD primers are getting > bands in there negative (water) controls, I thought I was the only > one. I to get wonderful bands in the negative controls that range from > about 200 base pairs - to as high as 1800 base pairs. I also must add to > say, that I got negative controls with some primers that had one bands > as high as 1000 base pairs, and other primers that provided me with a > multitude of bands. I am using primers from UBC, and I have tried a > number of various ways to account for the possible presence of the > bands: I changed the water of my negative control, fearing that > it may have been contaminated, I have changed the diltutions of the > DNA thinking that it may have been the DNA that was contaminated, but > I still had presence of these bands. I eventually used the Mermaid Kit > from BIO101, it consists of a silica base, that should help in the > purification of primer of at least 10 nucleotide base pair long. This > provided me with some success; however, the cleaning is not always that > effective, and sometimes the negative controls still showed presence > of bands. > > So I do not believe that absence of bands in the negative control > could not be present. > > > From owner-rapd@net.bio.net Thu Nov 23 22:00:00 1995 Path: biosci!MUSICB.MCGILL.CA!XKA5 From: XKA5@MUSICB.MCGILL.CA ("CAMPEOL,NADIA,MS") Newsgroups: bionet.molbio.rapd Subject: rapd artifactual bands Date: 24 Nov 1995 08:50:55 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <24NOV95.12764213.0359.MUSIC@MUSICB.MCGILL.CA> NNTP-Posting-Host: net.bio.net November 24, 1995 Well I am releaved that people using RAPD primers are getting bands in there negative (water) controls, I thought I was the only one. I to get wonderful bands in the negative controls that range from about 200 base pairs - to as high as 1800 base pairs. I also must add to say, that I got negative controls with some primers that had one bands as high as 1000 base pairs, and other primers that provided me with a multitude of bands. I am using primers from UBC, and I have tried a number of various ways to account for the possible presence of the bands: I changed the water of my negative control, fearing that it may have been contaminated, I have changed the diltutions of the DNA thinking that it may have been the DNA that was contaminated, but I still had presence of these bands. I eventually used the Mermaid Kit from BIO101, it consists of a silica base, that should help in the purification of primer of at least 10 nucleotide base pair long. This provided me with some success; however, the cleaning is not always that effective, and sometimes the negative controls still showed presence of bands. So I do not believe that absence of bands in the negative control could not be present. From owner-rapd@net.bio.net Fri Nov 24 22:00:00 1995 Path: biosci!KUCCNX.KOREA.AC.KR!jsshin From: jsshin@KUCCNX.KOREA.AC.KR (Jeong-Sheop Shin) Newsgroups: bionet.molbio.rapd Subject: (none) Date: 24 Nov 1995 21:49:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <9511250550.AA32198@kuccnx.korea.ac.kr> NNTP-Posting-Host: net.bio.net This is the reply for the artifactual bands problems using RAPD primers (XKA52@musicb.mcgill. ca: rapd artifactual bands). We have used UBC primers in watermelon, barley, wheat genome, etc. and my manuscript of "Detection of genetic diversity using RAPD-PCR and sugar analysis in watermelon" was recently accepted by TAG journal. We are always using UBC ten-mers to eliminate external contamination as follows; 1) purchase the commercial Taq polymerase which was the least contaminated bacterial DNA 2) add only the least amount of Taq polymerase required 3) use DEPC-treated water in all the reaction solution, even to dissolve and dilute the primers 4) work this experiment on clean-bench all the times 5) use the tips and tubes autoclaved 6) reduce the magnesium contents as possible As J. Carlson answered previously, I think that the enzyme is the main source of DNA contamination during the manufacturer's purification process. In our lab, we had not any artifactual bands shown except DNA-primer dimers using UBC primers (it would be the same if we use other 10-mers). However, you probably could try to use the decontamination method using ultra-violet inactivation as the papers of Nature Vol. 343, p27; Vol. 345, p773; Vol 347, p340, but we found these process would be useless. Therefore, please think about your enzyme and its concentration you have used, and DEPC-treated water at first. Thanks. from Dr. J.S. Shin from Korea. From owner-rapd@net.bio.net Mon Nov 27 22:00:00 1995 Path: biosci!EMAIL.AFIP.OSD.MIL!PARSONS_at_AFIP05 From: PARSONS_at_AFIP05@EMAIL.AFIP.OSD.MIL Newsgroups: bionet.molbio.rapd Subject: re: rapd artifactual bands Date: 28 Nov 1995 07:29:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 63 Sender: daemon@net.bio.net Distribution: world Message-ID: <9510288175.AA817582844@email.afip.osd.mil> NNTP-Posting-Host: net.bio.net This thread pops up periodically, and I thought it was pretty much accepted that the common phenomenon of bands in RAPD negative controls is due to contaminating Thermus aquaticus DNA in the enzyme preparation. Using specific primers, this DNA wouldn't be a problem (unless you have "univeral" bacterial primers), but it makes itself evident with non-specific RAPD primers. It is also my impression that people don't worry about these bands too much (and in my experience they aren't much of a problem). They are faint and inconsistent, and do not interfere (in my experience) with reproducibility of RAPDs on your actual template. As long as your sample results are reproducible, and none of your sample bands correspond to the bands in the negative control, then you ought to be able to proceed without undue concern. Tom Parsons ______________________________ Forward Header __________________________________ Subject: rapd artifactual bands Author: ,XKA5@MUSICB.MCGILL.CA ("CAMPEOL,NADIA,MS") at INTERNET Date: 11/28/95 2:54 AM Received: by ccmail from padstars.afip.osd.mil From BIOSCI-REQUEST@net.bio.net X-Envelope-From: BIOSCI-REQUEST@net.bio.net Received: from net.bio.net by padstars.afip.osd.mil with SMTP (16.6/15.6) id AA01000; Sat, 25 Nov 95 16:48:04 -0500 Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id IAA29488; Fri, 24 Nov 1995 08:50:59 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id IAA29484; Fri, 24 Nov 1995 08:50:58 -0800 To: rapd@net.bio.net From: XKA5@MUSICB.MCGILL.CA ("CAMPEOL,NADIA,MS") Subject: rapd artifactual bands Date: 24 Nov 1995 08:50:55 -0800 Sender: daemon@net.bio.net Message-Id: <24NOV95.12764213.0359.MUSIC@MUSICB.MCGILL.CA> Nntp-Posting-Host: net.bio.net November 24, 1995 Well I am releaved that people using RAPD primers are getting bands in there negative (water) controls, I thought I was the only one. I to get wonderful bands in the negative controls that range from about 200 base pairs - to as high as 1800 base pairs. I also must add to say, that I got negative controls with some primers that had one bands as high as 1000 base pairs, and other primers that provided me with a multitude of bands. I am using primers from UBC, and I have tried a number of various ways to account for the possible presence of the bands: I changed the water of my negative control, fearing that it may have been contaminated, I have changed the diltutions of the DNA thinking that it may have been the DNA that was contaminated, but I still had presence of these bands. I eventually used the Mermaid Kit from BIO101, it consists of a silica base, that should help in the purification of primer of at least 10 nucleotide base pair long. This provided me with some success; however, the cleaning is not always that effective, and sometimes the negative controls still showed presence of bands. So I do not believe that absence of bands in the negative control could not be present. From owner-rapd@net.bio.net Thu Nov 30 22:00:00 1995 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!vixen.cso.uiuc.edu!robertson2.life.uiuc.edu!user From: ramsdell@pop.life.uiuc.edu (karlene ramsdell) Newsgroups: bionet.molbio.rapd Subject: review of rapd usage Followup-To: bionet.molbio.rapd Date: Fri, 01 Dec 1995 14:12:20 -0600 Organization: U of Illinois Lines: 4 Message-ID: NNTP-Posting-Host: robertson2.life.uiuc.edu Does anyone know of work done on parasitic hymenopterans using rapds or microsatellites for identification of biotypes? I'm especially interested in braconids. I'm also interested in any work done using these methods on other insects.