From owner-rapd@net.bio.net Wed Jan 03 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!agate!dog.ee.lbl.gov!news.cs.utah.edu!swen.emba.uvm.edu!newsmaster From: alamarch@moose.uvm.edu (alamarch) Newsgroups: bionet.molbio.rapd Subject: Re: maize RAPD Date: 3 Jan 1996 20:35:26 GMT Organization: University of Vermont Lines: 16 Message-ID: <4cepae$5ta@swen.emba.uvm.edu> References: <4b6bsl$724@bee.uspnet.usp.br> NNTP-Posting-Host: 132.198.177.29 X-Posted-From: InterNews 1.0.7@132.198.177.29 X-Authenticated: alamarch on POP host moose.uvm.edu I have not seen anything specific to maize using RAPDS. here is a genereral reference for RAPDs Nucleic Acid Research Vol 18 No 22 pgs 6531 - 6535 I also have a reference for PCR using simple sequence repeats. One of the organisms the authors used was maize Theor. Appl. Genet. (1994) 89:898-1006 I hope this gets you started art alamarch@moose.uvm.edu From owner-rapd@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!agate!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!tank.news.pipex.net!pipex!oleane!nntp.coast.net!harbinger.cc.monash.edu.au!bunyip.cc.uq.oz.au!news From: Mark Fegan Newsgroups: bionet.molbio.rapd Subject: Contamination problems Date: Mon, 08 Jan 1996 10:28:32 -0800 Organization: University of Queensland Lines: 8 Message-ID: <30F16250.3B61@biosci.uq.oz.au> NNTP-Posting-Host: 130.102.116.164 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0b4a (Win16; I) Does anyone out there know of a reference for contamination problems due to carryover E. coli DNA in the Taq polymerase causing baands to appear in the negative controls Thanks Mark From owner-rapd@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!agate!howland.reston.ans.net!tank.news.pipex.net!pipex!sunsite.doc.ic.ac.uk!daresbury!not-for-mail From: "Moira van Staaden" Newsgroups: bionet.molbio.rapd Subject: Polyerases Date: 8 Jan 1996 15:44:40 -0000 Lines: 29 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4cre58$ij6@mserv1.dl.ac.uk> Reply-To: "Moira van Staaden" Original-To: rapd@dl.ac.uk Hi Netters, The odd band from bacterial contamination of Taq polmerase is to be expec= ted in = a RAPD negative control but recent lots from my current supplier have bec= ome = progressively worse - I need to change. Is there a consensus on which sup= plier = consistently maintains *low* levels of bacterial contamination? Thanks, Moira Dr. Moira J. van Staaden = Institut f=9Fr Zoologie Karl-Franzens-Universit=8At Graz = Universit=8Atsplatz 2 Nkosi Sikelel'i Afrika A-8010 Graz Austria Tel: (316) 380 5281 Fax: (316) 381 255 email: m.van-staaden@kfunigraz.ac.at From owner-rapd@net.bio.net Sun Jan 07 22:00:00 1996 Path: biosci!u-shizuoka-ken.ac.jp!hirokazu From: hirokazu@u-shizuoka-ken.ac.jp (Hirokazu KOBAYASHI) Newsgroups: bionet.molbio.rapd Subject: Re: Contamination problems Date: 8 Jan 1996 04:31:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 66 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601081229.VAA14555@fns1.u-shizuoka-ken.ac.jp> NNTP-Posting-Host: net.bio.net The article attached below obtained from the same newsgroup long time ago might help you. I am sure that it was also my case, and so I bought a new autoclave for ware for PCR. At 10:28 AM 96.1.8 -0800, Mark Fegan wrote: >Does anyone out there know of a reference for contamination problems due >to carryover E. coli DNA in the Taq polymerase causing baands to appear >in the negative controls > > >Thanks > >Mark Hirokazu Kobayashi e-mail: hirokazu@u-shizuoka-ken.ac.jp e-mail: hirokazu@nibb.ac.jp --------------------------------------- From BIOSCI-REQUEST@net.bio.net Mon Aug 15 20:50:21 1994 To: rapd@net.bio.net From: osp086@clss1.bangor.ac.uk Subject: bands in the blank Date: 15 Aug 1994 10:56:47 +0100 Sender: lpddist@mserv1.dl.ac.uk Message-Id: <32ne4v$4p3@mserv1.dl.ac.uk> Original-To: rapd@dl.ac.uk Status: R Michael (?) [mam4339@tamsun.tamu.edu] wrote: This is probably a question that gets asked alot here, but I need some help so I'm going to ask it again. My blanks have been showing bands since the first time I tried RAPDs in my lab. In another building I could do them without contamination, apparently, but here, or with the reagents here, I find big, bright, numerous bands in my blanks. I have received advice on the matter and have tried to make use of it but to no avail. I realize that the technique I use is not the absolute best, in terms of contamination, as I have heard of the incredibly sterile, careful facilities and procedures of other, successful labs. But I don't think that kind of care is feasible in the lab I work in, both monetarily and in the interest of time. [stuff deleted] I remember reading a similar query sometime ago on the newsgroup (no idea who from), and one potential reason for these bands in the blanks concerned autoclaving. The gist of it was that if you autoclave tips, tubes etc. in the same autoclave that microbiological samples are done in then their DNA may get coated over your stuff and amplify up later. The sender had said that he/she stopped autoclaving and hey presto! the bands went. No idea if this was true or whether I presented their answer properly but hope it helps. ********************************************************************* * CRAIG WILDING, E-mail: OSP086@BANGOR.AC.UK * * SCHOOL OF OCEAN SCIENCES, Phone: (0)248 351151 ext.2899 * * UNIVERSITY OF WALES (BANGOR), Fax:(0)248 761367 * * MENAI BRIDGE, * * GWYNEDD, LL59 5EY, * * WALES, U.K. * ********************************************************************* From owner-rapd@net.bio.net Mon Jan 08 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.reston.ans.net!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: Jonathan Redfern <100537.271@CompuServe.COM> Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.rapd Subject: Re: In situ/ tubes Thermal Cycler? Date: 9 Jan 1996 17:13:59 GMT Organization: Techne (Cambridge) Ltd Lines: 25 Message-ID: <4cu7on$2al$1@mhade.production.compuserve.com> References: Xref: biosci bionet.molbio.methds-reagnts:38412 bionet.molbio.rapd:1420 Dear Mr Richburg, I hope you find the following useful. RE: PCR machine with multiple blocks for tubes and slides. One option would be to use the new TECHNE Thermal Cycler called the PROGENE this machine is not only the fastest tube cycler in the world (3°C/Sec) but is also the smallest! A setup offering 20x0.5ml tubes and at the same time an 'In-situ' block for 2 slides would cost around $4800, thats complete with heated lid and the blocks are all interchangeable if you should later wish to use 0.2ml tubes or a mini-microplate. If you should wish for further details please contact me. More information can be found on the BIOMEDNET (www.cursci.com) under 'Thermal Cyclers' (Please note the Slide option is not included in the BioMedNet as it is not currently in our catalogue) Jon Redfern Export Product Manager TECHNE (Cambridge) Ltd. / TECHNE (Princeton) Inc. Export Product Manager TECHNE (Cambridge) Ltd. / TECHNE (Princeton) Inc. From owner-rapd@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!agate!dog.ee.lbl.gov!news.cs.utah.edu!swen.emba.uvm.edu!newsmaster From: alamarch@moose.uvm.edu (alamarch) Newsgroups: bionet.molbio.rapd Subject: Re: Contamination problems Date: 9 Jan 1996 13:33:30 GMT Organization: University of Vermont Lines: 9 Distribution: world Message-ID: <4ctqra$27o@swen.emba.uvm.edu> References: <199601081229.VAA14555@fns1.u-shizuoka-ken.ac.jp> NNTP-Posting-Host: 132.198.177.29 X-Posted-From: InterNews 1.0.7@132.198.177.29 X-Authenticated: alamarch on POP host moose.uvm.edu I, also, have had a problem with contamination. I believe part of my problem is the high static electricity charging that occurs in the Winter. I have noticed that dust, hair and lint are attracted to the Pipettemen. I have been rinsing them with ethanol before each use. It seems to help. Does anyone else have any suggestions to try? art alamarch@moose.uvm.edu From owner-rapd@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!greb.ulaval.ca!Christian.Mouton From: Christian.Mouton@greb.ulaval.ca (Christian Mouton) Newsgroups: bionet.molbio.rapd Subject: Re: Rapd programs for mac Date: 10 Jan 1996 14:49:23 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <9601102249.AA08407@hermes.ulaval.ca> NNTP-Posting-Host: net.bio.net >Does anyone know of any on the net? I keep finding on PC versions > > >W/regards, > >Bryan Check with Patrick A. D. Grimont (pgrimont@pasteur.fr ) who wrote a series of softwares (RestrictoScan RestrictoTyper...) which can be used for analysis of RAPD fingerprints. The software is commercialized by the Pasteur Institute, Paris. Regards, Christian Mouton Groupe de Recherche en Ecologie Buccale Faculte de medecine dentaire, Universite Laval Quebec (Quebec) G1K 7P4 CANADA tel. (418)656-5872; fax. (418)656-2861 From owner-rapd@net.bio.net Tue Jan 09 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!newsrelay.netins.net!usenet.ee.pdx.edu!odin.cc.pdx.edu!NewsWatcher!user From: psu05565@odin.cc.pdx.edu (Bryan Grieg Fry) Newsgroups: bionet.molbio.rapd Subject: Rapd programs for mac Date: Wed, 10 Jan 1996 08:34:36 -0800 Organization: PSU Lines: 6 Message-ID: NNTP-Posting-Host: 131.252.184.61 Does anyone know of any on the net? I keep finding on PC versions W/regards, Bryan From owner-rapd@net.bio.net Wed Jan 10 22:00:00 1996 Path: biosci!ODIN.CC.PDX.EDU!psu05565 From: psu05565@ODIN.CC.PDX.EDU ("Bryan Grieg Fry......Mad-Scientist at Large") Newsgroups: bionet.molbio.rapd Subject: (none) Date: 11 Jan 1996 09:48:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net please subsribe me psu05565@odin.cc.pdx.edu From owner-rapd@net.bio.net Thu Jan 11 22:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!swidir.switch.ch!swsbe6.switch.ch!news.vub.ac.be!ben.vub.ac.be!wmoens From: William Moens Newsgroups: bionet.molbio.rapd Subject: Re: Contamination problems Date: Fri, 12 Jan 1996 21:21:57 +0000 Organization: Brussels Free Universities VUB/ULB Lines: 27 Message-ID: References: <30F16250.3B61@biosci.uq.oz.au> NNTP-Posting-Host: ben.vub.ac.be. Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <30F16250.3B61@biosci.uq.oz.au> We had met the same problems in the negative controls but rarely in the test samples. We work systematically under vertical lamina flow hood in a dedicated PCR room. But the conviction of the skillest experimentators of the lab is that manipulation should occur on a bed of ice dispersed in a kind of photoographic development tray. Why? They say it creates a area of cool air (thus heavier) above the tubes reducing the dust movements.The second care is that none of the tubes is left opened once used.Although we all know that PCR mixes can be prepared at room temperature, it happens with certain primers that RAPD is more reproducable if all the mix is kept at 0 degree before the first denaturation. Take it as I see it daily. And the results are clean. I do not believe in voodoo tricks, only in agarose patterns and reproducability. Cheers, William Moens On Mon, 8 Jan 1996, Mark Fegan wrote: > Does anyone out there know of a reference for contamination problems due > to carryover E. coli DNA in the Taq polymerase causing baands to appear > in the negative controls > > > Thanks > > Mark > > From owner-rapd@net.bio.net Sun Jan 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!newsfeed.internetmci.com!newsxfer2.itd.umich.edu!newsxfer.itd.umich.edu!news2.acs.oakland.edu!nntp.coast.net!torn!ccshst05.cs.uoguelph.ca!ccshst01.cs.uoguelph.ca!mgatt From: mgatt@uoguelph.ca (Michael Gatt) Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.rapd,bionet.organisms.zebrafish Subject: extractin mtDNA from fish scales Date: 15 Jan 1996 20:35:11 GMT Organization: University of Guelph Lines: 18 Message-ID: <4dedpv$2h7@ccshst05.cs.uoguelph.ca> NNTP-Posting-Host: ccshst01.cs.uoguelph.ca X-Newsreader: TIN [version 1.2 PL2] Xref: biosci bionet.molbio.methds-reagnts:38672 bionet.molbio.rapd:1426 bionet.organisms.zebrafish:516 I am trying to extract mtDNA from walleye scales in sufficient quantity and quality which can be used in a Polymerase Chain Reaction. So far I have used a standard DNA extraction protocol that involves using 6-8 scales incubated over night at 60 C in Proteinase K and buffer 10mM Tris ph 8.3, 50mM KCl, 0.5% Tween 20. The samples were then heated at 95C to inactivate the remaining proteinase K. After that I PCRed as I would with other mtDNA samples extracted from tissue. My control sample worked but the DNA from the scales didn't. I am not sure whether I need to try phenol-chloroforming (it has been recommended that I don't do this) or whether this would reduce the small concentration of DNA already present. Any information on other ways that I may get this to work would be appreciated. Or maybe one will have recommendations on ways to inprove the present technique that I used. Mike Gatt Department of Zoology University of Guelph Guelph, Canada From owner-rapd@net.bio.net Tue Jan 16 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 17 Jan 1996 02:00:27 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199601171000.CAA06829@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. 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For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-rapd@net.bio.net Tue Jan 16 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!howland.reston.ans.net!lamarck.sura.net!csc.mc.edu!usenet From: mobo@mc.edu Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.rapd,bionet.organisms.zebrafish Subject: Re: extractin mtDNA from fish scales Date: 17 Jan 1996 21:35:32 GMT Organization: Mississippi College Lines: 8 Message-ID: <4djq34$m20@csc.mc.edu> References: <4dedpv$2h7@ccshst05.cs.uoguelph.ca> NNTP-Posting-Host: mobo.mc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) To: mgatt@uoguelph.ca Xref: biosci bionet.molbio.methds-reagnts:38805 bionet.molbio.rapd:1428 bionet.organisms.zebrafish:517 First, If you used SDS, it is a strong enzyme inhibitor. I use CTAB, and get better results. I have a particularly tough template..ressurection fern...that has yet to give me good results. I am going to try some column purification techniques, first using a genomic DNA isolation kit purchased from Pharmacia (27-5225-01). There was a good DNA extraction method in a paper by Hadrys..or Hadrys was an author in Molecular Ecology..I think in 1993. From owner-rapd@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!portal.gmu.edu!hearst.acc.Virginia.EDU!news.jmu.edu!pasfiecm From: pasfiecm@falcon.jmu.edu (Curtis M. Pasfield) Newsgroups: bionet.molbio.rapd Subject: Insect DNA prep Date: 19 Jan 1996 00:40:10 GMT Organization: James Madison University, Harrisonburg, VA Lines: 12 Sender: pasfiecm@jmu.edu Message-ID: <4dmp9a$oib@doc.jmu.edu> NNTP-Posting-Host: falcon.jmu.edu X-Newsreader: TIN [version 1.2 PL2 JMU4] I am looking for a inexpensive protocol for isolating genomic DNA from Homoptera. I was wondering if there are any good references for preparation of Insect DNA for all stages in the life cycle. The DNA will be used for RAPD- PCR and eventually RFLP analysis. I have found several possibilitys but I am still looking for some practical ones. Thanks in advance. Curtis Pasfield Dept. of Biology James Madison University pasfiecm@jmu.edu From owner-rapd@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!ASRR.ARSUSDA.GOV!abartlet From: abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") Newsgroups: bionet.molbio.rapd Subject: Re: Insect DNA prep Date: 19 Jan 1996 06:57:39 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <4dmp9a$oib@doc.jmu.edu> NNTP-Posting-Host: net.bio.net We have been successfully extracting genomic DNA from whiteflies using the following protocol: Single insects are ground with a micropestle in a 1.5 ml microcentrifuge tube containing 60 ul lysis buffer. The buffer is composed of 5mM Tris HCl (pH 8.0), 0.5 mM EDTA, 0.5% Nonidet P-40, and 1 mg/ml proteinase K. You can see some our results in Insect Molecular Biology (1993) 2:33-38 (Gawel & Bartlett). If you give me your snail mail address I will send a reprint. Good luck! On 19 Jan 1996, Curtis M. Pasfield wrote: > I am looking for a inexpensive protocol for isolating > genomic DNA from Homoptera. I was wondering if there are any good > references for preparation of Insect DNA for all stages in the life > cycle. The DNA will be used for RAPD- PCR and eventually RFLP analysis. I > have found several possibilitys but I am still looking for some practical > ones. Thanks in advance. > > Curtis Pasfield > Dept. of Biology > James Madison University > pasfiecm@jmu.edu > > > Alan C. Bartlett "GENES-R-US" "ALTERATIONS AVAILABLE!^" "^some restrictions may apply:)" From owner-rapd@net.bio.net Thu Jan 18 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!newsrelay.netins.net!news.dacom.co.kr!nntp.coast.net!harbinger.cc.monash.edu.au!newshost.anu.edu.au!newsmaster From: John Armstrong Newsgroups: bionet.molbio.rapd Subject: Re: Rapd programs for mac Date: 19 Jan 1996 05:18:25 GMT Organization: Australian National University Lines: 5 Message-ID: <4dn9j1$j7s@manuel.anu.edu.au> References: NNTP-Posting-Host: 150.203.38.97 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) To: psu05565@odin.cc.pdx.edu The simplest solution is to use the emulation option that is available with the more powerful Macs. (Soft PC, Soft Pc Professional). We have developed a package called RAPDistance which many people have successfully used on Macs via emulation. Regards John A From owner-rapd@net.bio.net Mon Jan 22 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!usenet.eel.ufl.edu!psgrain!quagga.ru.ac.za!wabe.csir.co.za!foodtek.csir.co.za!jburger From: jburger@foodtek.csir.co.za (Johan Burger) Newsgroups: bionet.molbio.rapd Subject: Nematode DNA extraction Date: Tue, 23 Jan 1996 09:08:09 LOCAL Organization: FOODTEK (CSIR) Lines: 9 Message-ID: NNTP-Posting-Host: 146.64.120.62 X-Newsreader: Trumpet for Windows [Version 1.0 Rev B final beta #4] I am looking for a reliable protocol to extract genomic DNA from formaldehyde-preserved nematode samples. We want to do RAPD-PCR on these. Can anyone please assist. Thanks Johan Burger From owner-rapd@net.bio.net Tue Jan 23 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!chi-news.cic.net!nntp.coast.net!torn!news.acs.ryerson.ca!news.acs.ryerson.ca!not-for-mail From: c3chen@acs.ryerson.ca (PHAS/W94) Newsgroups: bionet.molbio.rapd Subject: test Date: 24 Jan 1996 00:19:26 -0500 Organization: Ryerson Polytechnic University Lines: 1 Message-ID: <4e4fgu$5gg2@hopper.acs.ryerson.ca> NNTP-Posting-Host: 141.117.101.8 X-Newsreader: TIN [version 1.2 PL2] testing.. From owner-rapd@net.bio.net Wed Jan 24 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!howland.reston.ans.net!ix.netcom.com!netnews From: Dan Tisone Newsgroups: bionet.molbio.rapd Subject: WEB SITE - Equipment for rapid test kits Date: Wed, 24 Jan 1996 19:25:59 -0800 Organization: Netcom Lines: 3 Message-ID: <3106F847.5E95@ix.netcom.com> NNTP-Posting-Host: irv-ca5-22.ix.netcom.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-NETCOM-Date: Wed Jan 24 7:23:54 PM PST 1996 X-Mailer: Mozilla 2.0b5 (Win95; I) There is a company, BioDot, who manufactures equipment for the rapid test kit market. Their web site, http://www.biodot.com/testkit, has pictures of some of their products if anyone is interested. From owner-rapd@net.bio.net Mon Jan 29 22:00:00 1996 Path: biosci!MTU.EDU!cpjoshi From: cpjoshi@MTU.EDU (cpjoshi) Newsgroups: bionet.molbio.rapd Subject: postdoc positon Date: 30 Jan 1996 15:16:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Postdoctoral Position in differential display Michigan Technological University, Houghton, Michigan A postdoctoral research associate position is available immediately at the Plant Biotechnology Research Center of Michigan Technological University. This project deals with cloning, characterization and manipulation of genes that are associated with fast growth of a tree species. Candidates should have a Ph.D. in plant biochemistry or plant physiology with experience in recombinant DNA techniques as evidenced by research publications. Expertise in differential display, plant physiology or protein biochemistry will be desirable. Send curriculum vitae, a statement of research experience and names, addresses and telephone/fax numbers of three references to: Dr. Vincent Chiang or Dr. C. P. Joshi Institute of Wood Research School of Forestry Michigan Technological University Houghton, MI 49931 Tel. 906-487-3480, email Vchiang@mtu.edu or cpjoshi@mtu.edu Dr. C.P. Joshi Department of Forestry Michigan Technological University Houghton, MI 49931 Tel.: 906-487-3480 Fax: 906-487-2915 email cpjoshi@mtu.edu From owner-rapd@net.bio.net Tue Jan 30 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Roland Koelliker Newsgroups: bionet.molbio.rapd Subject: Gel analysis using the GelCompar program Date: 31 Jan 1996 06:37:06 -0000 Lines: 14 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4en2mi$64i@mserv1.dl.ac.uk> X-Sender: rkoellik@patho1 content-length: 407 Original-To: rapd@dl.ac.uk Is anybody familiar with the GelCompar program of Applied Maths in Belgium and can give me some assistance? Thank you for your help! Roland Roland Koelliker Swiss Fed Inst Technol Plant Sciences ETH-Zentrum, LFW-C47 CH-8092 Zurich Tel ++41-1-632 4890 Fax ++41-1-632 1153 e-mail: koelliker@ipw.agrl.ethz.ch From owner-rapd@net.bio.net Tue Jan 30 22:00:00 1996 Path: biosci!UOW.EDU.AU!Al_Griskaitis From: Al_Griskaitis@UOW.EDU.AU ("Al Griskaitis") Newsgroups: bionet.molbio.rapd Subject: microsatellites Date: 30 Jan 1996 19:52:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Fellow RAPDers, Having successfully worked with RAPDs, our lab is now considering utilising microsatellites. So may I pose a few requests to fellow researchers with more insight to microsatellites than myself: 1. Does anyone know of a microsatellite bulletin board equivalent to this RAPDs one? 2. Can anyone reading this furnish me with a detailed microsatellite protocol (from DNA to data analysis software) 3. A list of materials/apparatus and suppliers they'd recommend. I'd greatly appreciate ANY assistance. Thanks, Al. From owner-rapd@net.bio.net Wed Jan 31 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "J. Cameron" Newsgroups: bionet.molbio.rapd Subject: RAPD analysis Date: 1 Feb 1996 15:06:55 -0000 Lines: 14 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4eqkuf$n4r@mserv1.dl.ac.uk> MIME-Version: 1.0 Original-To: rapd@dl.ac.uk SOS Does anyone in the UK, preferably Midlands-ish, have a working copy of RAPDistance or other analysis package. If so would it be possible to let me have a copy, or better still allow me to pay a brief visit to analyse one small data set? I have obtained a copy of RAPDistance from John Armstrong, but am having trouble getting it to work, and I need to look at my data urgently. Many thanks Janet Cameron Keele University