From owner-rapd@net.bio.net Wed May 01 23:00:00 1996 Path: biosci!TTACS1.TTU.EDU!brshp From: brshp@TTACS1.TTU.EDU (Ping Song) Newsgroups: bionet.molbio.rapd Subject: TMAC Date: 2 May 1996 15:58:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I48M6Q5OYQ8WZURY@ttacs.ttu.edu> NNTP-Posting-Host: net.bio.net At 11:20 PM 5/1/96 -0500, you wrote: >>Regardless wew cloned 1 of the distinguishing bands and typical luck, it >> was AT rich and would only amplify when I added TMAC in with the selected >> primers. > >Steve, what is TMAC? Sorry I don't have much to contribute but interesting >post though. > >******************** >Shiao Y. Wang >Univ. of Southern Mississippi >sywang@whale.st.usm.edu > Hey, Wang, TMAC is tetramethylammonium chloride. As far as I know, it can increase the priming specificity when added into PCR reaction. Ping Ping Song, Ph.D. Research Associate brshp@ttacs.ttu.edu http://pegasus.acs.ttu.edu/~brshp (806)742-2709, FAX (806)742-2963 From owner-rapd@net.bio.net Wed May 01 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!newsserver.jvnc.net!newsserver2.jvnc.net!cpk-news-hub1.bbnplanet.com!thorn.cc.usm.edu!news From: Shiao Wang Newsgroups: bionet.molbio.rapd Subject: Re: RAPD / AP PCR and Context effect Date: Wed, 01 May 1996 23:20:25 -0500 Organization: University of Southern Mississippi Lines: 11 Message-ID: <31883809.2748@whale.st.usm.edu> References: <4m06nv$gk6@sol.sun.csd.unb.ca> NNTP-Posting-Host: 131.95.113.27 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win95; I) >Regardless wew cloned 1 of the distinguishing bands and typical luck, it > was AT rich and would only amplify when I added TMAC in with the selected > primers. Steve, what is TMAC? Sorry I don't have much to contribute but interesting post though. ******************** Shiao Y. Wang Univ. of Southern Mississippi sywang@whale.st.usm.edu From owner-rapd@net.bio.net Thu May 09 23:00:00 1996 Path: biosci!UBVMS.CC.BUFFALO.EDU!COFFROTH From: COFFROTH@UBVMS.CC.BUFFALO.EDU (Mary Alice Coffroth) Newsgroups: bionet.molbio.rapd Subject: AFLP and RAMPS Date: 10 May 1996 11:24:53 -0700 Organization: University at Buffalo Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I4JL0KXYMA8XZHIZ@ubvms.cc.buffalo.edu> NNTP-Posting-Host: net.bio.net Hi! As many of you may remember from a previous posting, I am putting together a mini-review of molecular techniques that are useful in the study of clonal organisms. Thanks to many of you I am come up with quite a list of acronyms. Some of the techniques probably won't have the power to resolve individual genotypes, but many look quite useful and some have been used already to distinguish genotypes. I am now sorting through them all for examples to use. Some of the methods have been used to distinguish genotypes, but some techniques are relatively new (AFLP and RAMP) and I was wondering if anyone is aware of studies that have looked at the ability of these methods to resolve genotypes. Both approaches seem appropriate for this level of analysis, but it would be nice to be able to present some data (i.e., sampled xx individuals, identified xx clones, etc.) Again, any input would be greatly appreciated, and thanks again to all those who have provided me with acronyms. Cheers, Mary-Alice Coffroth * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Mary-Alice Coffroth Department of Biological Sciences State University of New York at Buffalo Buffalo, NY 14260 U.S.A. Email: coffroth@ubvms.cc.buffalo.edu Phone: 716-645-2881 Fax:716-645-2975 * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * From owner-rapd@net.bio.net Sun May 12 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!news.uoregon.edu!newsfeed.orst.edu!news.orst.edu!ucs.orst.edu!rosatoc From: Caprice Rosato Newsgroups: bionet.molbio.rapd Subject: Dendron Date: Mon, 13 May 1996 12:33:31 -0700 Organization: Oregon State University Lines: 3 Message-ID: References: <4m06nv$gk6@sol.sun.csd.unb.ca> <31883809.2748@whale.st.usm.edu> NNTP-Posting-Host: ucs.orst.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <31883809.2748@whale.st.usm.edu> Has anyone had any experience with this analysis software? The promotional literature is appealing albeit spendy. From owner-rapd@net.bio.net Mon May 13 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!dimensional.com!winternet.com!newsfeed.concentric.net!cdc2.cdc.net!vixen.cso.uiuc.edu!uwm.edu!fnnews.fnal.gov!nntp-server.caltech.edu!news.pomona.edu!usenet From: Mira Liao Newsgroups: bionet.molbio.rapd Subject: rapd and molluscs Date: Tue, 14 May 1996 12:24:10 -0800 Organization: Pomona College Lines: 10 Message-ID: <3198EBEA.6CAE@pomona.edu> NNTP-Posting-Host: gclass8.pomona.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01 (Macintosh; I; PPC) A couple of months ago, I posted a request for mollusc DNA extraction techniques to use for my senior thesis. Thank you very much to everyone who wrote back. I decided to try Harry Witchel's version of the Winnepenninckx, et al. technique from TIGS 1993, which was sent to me by Elizabeth Boulding. I was not able to get RAPD amplification results after extracting with this technique, and I was unable to try other techniques because I ran out of time. Again, thank you for all of the responses. I am amazed at the power of this medium for accessing information. --Mira Liao From owner-rapd@net.bio.net Tue May 14 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Ivo Wiesner Newsgroups: bionet.molbio.rapd Subject: [Q]: best restrictase for PCR cloning Date: 15 May 1996 15:07:54 +0100 Lines: 12 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4ncofq$ap7@mserv1.dl.ac.uk> MIME-Version: 1.0 Original-To: rapd@dl.ac.uk Dear Colleagues, which restriction enzyme is by your experience the best for cloning RAPD, resp. PCR products and how far from 5'-end restriction site sequence should be for full restriction processing? ThanX much for info or further pointer(s). Ivo Wiesner nfix@jcu.cz From owner-rapd@net.bio.net Tue May 14 23:00:00 1996 Path: biosci!daresbury!lyra.csx.cam.ac.uk!warwick!bham!bcs152.bham.ac.uk!user From: C.Constantinidou@bham.ac.uk (Chrystala Constantinidou) Newsgroups: bionet.molbio.rapd Subject: Re: AFLP and RAMPS Date: 15 May 1996 16:08:41 GMT Organization: University of Birmingham Lines: 45 Distribution: world Message-ID: References: <01I4JL0KXYMA8XZHIZ@ubvms.cc.buffalo.edu> NNTP-Posting-Host: bcs152.bham.ac.uk In article <01I4JL0KXYMA8XZHIZ@ubvms.cc.buffalo.edu>, COFFROTH@UBVMS.CC.BUFFALO.EDU (Mary Alice Coffroth) wrote: > Hi! > > As many of you may remember from a previous posting, I am putting > together a mini-review of molecular techniques that are useful in the study > of clonal organisms. Thanks to many of you I am come up with quite a list > of acronyms. Some of the techniques probably won't have the power to resolve > individual genotypes, but many look quite useful and some have been used > already to distinguish genotypes. I am now sorting through them all > for examples to use. Some of the methods have been used to distinguish > genotypes, but some techniques are relatively new (AFLP and RAMP) and I was > wondering if anyone is aware of studies that have looked at the ability of > these methods to resolve genotypes. Both approaches seem appropriate for > this level of analysis, but it would be nice to be able to present some > data (i.e., sampled xx individuals, identified xx clones, etc.) > > > Again, any input would be greatly appreciated, and thanks again to > all those who have provided me with acronyms. > > Cheers, > > Mary-Alice Coffroth > > * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * > > Mary-Alice Coffroth Could you tell me what AFLP and RAMP is please? Regards Wig > > Department of Biological Sciences > State University of New York at Buffalo > Buffalo, NY 14260 U.S.A. > > Email: coffroth@ubvms.cc.buffalo.edu > Phone: 716-645-2881 > Fax:716-645-2975 > > * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * From owner-rapd@net.bio.net Thu May 16 23:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!sgigate.sgi.com!esiee.fr!jussieu.fr!univ-lyon1.fr!in2p3.fr!swidir.switch.ch!scsing.switch.ch!elna.ethz.ch!usenet From: Daniel Frey Newsgroups: bionet.molbio.rapd Subject: Re: Dendron Date: Fri, 17 May 1996 20:03:46 +0200 Organization: ETH Zürich Lines: 13 Message-ID: <319CBF82.1C01@umnw.ethz.ch> References: <4m06nv$gk6@sol.sun.csd.unb.ca> <31883809.2748@whale.st.usm.edu> Reply-To: frey@umnw.ethz.ch NNTP-Posting-Host: dokos.ethz.ch Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Yes. I tested a demonstration version of Dendron. It was version 2.0 if I remember correctly. Anyway, database handling is very poor. Ie marker weight with more than 5 characters cannot be handled correctly. The whole database is a textfile, so no sql queries possible, and I don't know how it behaves with large amount of data. The overall aspect of the package convinced me, but the developing state was something like in a gamma- or beta-phase. A unique feature of the package which I miss in others is the fact, that different lanes out of different gels can be aligned into a virtual gel. That's a great point! If you are interested in getting more information, just email me to frey@umnw.ethz.ch. I had a big conversation with the developpers going into some more details. From owner-rapd@net.bio.net Thu May 16 23:00:00 1996 Path: biosci!daresbury!imbb1.imbb.forth.gr!nefeli.imbb.forth.gr!ANASTASO From: anastaso@nefeli.imbb.forth.gr Newsgroups: bionet.molbio.rapd Subject: tobacco mutant seeds Date: Fri, 17 May 1996 17:19:51 GMT Organization: Imbb-forth,CREtE, creece Lines: 32 Message-ID: <009A276B.3AFD178D@nefeli.imbb.forth.gr> Reply-To: anastaso@nefeli.imbb.forth.gr NNTP-Posting-Host: nefeli.imbb.forth.gr Dear Netters, we are to mutagenise TOBACCO seeds and then select for the mutants In order to map the position of the mutation we would need tobacco plants that carry known markers; especially ones that are known to reside at particular chromosomes would be very usefull to our mapping effort. Therefore may we ask anyone who has seeds from such plants to send us some to the address mentioned below. Please note that this message had to be reposted because of a failure to our VAX system that didnt allow us to recieve any responses. Thank you for your help. Elias Anastassopoulos, Foundation of Research and Technology-Hellas Institute of Molecular Biology and Biotechnology P.O.Box 1527, Heraklion, 711 10, Crete, HELLAS. Fax # (081) 230469 email:anastaso@nefeli.imbb.forth.gr From owner-rapd@net.bio.net Thu May 16 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 17 May 1996 02:00:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605170900.CAA04646@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-rapd@net.bio.net Fri May 17 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!newshub.csu.net!bobcat.csusm.edu!gibso001 From: gibso001@csusm.edu (Hal Gibson) Newsgroups: bionet.molbio.rapd Subject: bluegill rapd's Date: 18 May 1996 05:22:20 GMT Lines: 1 Message-ID: <4njmqc$j0k@bobcat.csusm.edu> NNTP-Posting-Host: san_marcos.csusm.edu X-Newsreader: TIN [version 1.2 PL2] From owner-rapd@net.bio.net Sun May 19 23:00:00 1996 Path: biosci!AC.DAL.CA!har1 From: har1@AC.DAL.CA ("Harold A. Robertson") Newsgroups: bionet.molbio.rapd Subject: "Differential Display And Related Techniques For Gene Discovery" Date: 20 May 1996 16:00:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605202257.TAA13395@Snoopy.UCIS.Dal.Ca> NNTP-Posting-Host: net.bio.net The 2nd International Meeting on "Differential Display And Related Techniques For Gene Discovery" will take place at COLD SPRING HARBOR LABORATORY, COLD SPRING HARBOR, NY. Time: October 6-9, 1996 Advertisements will appear soon in NATURE and CELL. Deadline for abstracts will be 26 JULY, 1996. ORGANISERS: Peng Liang (Vanderbilt Cancer Center)(peng.liang@mcmail.vanderbilt.edu) Harry Robertson (Dalhousie University)(harold.robertson@dal.ca) Speakers include: Arthur Pardee, Harvard Medical School, Boston, MA Takashi Ito, University of Tokyo, Japan Sean McCarthy, Millenium Pharmaceuticals, Cambridge, MA Mark G. Erlander, R. W. Johnson Pharmaceutical Research Institute, San Diego, CA M. Jorge Guimaraes, DNAX Research Institute, Palo Alto, CA Charles J. Gomer, Children Hospital Los Angeles Jeffrey A. Winkles, American Red Cross Gunter Theissen, Max-Planck-Institute Cologne Tadeusz W. Wieloch, Lund University, Sweden Mary Russell, Harvard School of Public Health Michael McClelland, Sidney Kimmel Cancer Center, San Diego, CA Giora Feuerstein, Smithkline Beecham, King of Prussia, PA Brian Perry, Genomyx Inc., Foster City, CA Sherman Weissman, Yale University School of Medicine Information from: Cold Spring Harbor Laboratory 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA tel 516 367-8346 fax 516 367-8845 email: meetings@cshl.org http://www.chsl.org/meetings/ From owner-rapd@net.bio.net Tue May 21 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!univ-lyon1.fr!in2p3.fr!swidir.switch.ch!swsbe6.switch.ch!surfnet.nl!howland.reston.ans.net!newsfeed.internetmci.com!uwm.edu!vixen.cso.uiuc.edu!newsfeed.ksu.ksu.edu!news.cis.okstate.edu!David.Demezas From: demed@vms.ucc.okstate.edu. (David Demezas) Newsgroups: bionet.molbio.rapd Subject: Re: Dendron Date: 20 May 1996 21:51:15 GMT Organization: Oklahoma State University, Stillwater OK Lines: 2 Sender: -Not-Authenticated-[3075] Message-ID: <4nqpgj$sup@news.cis.okstate.edu> References: <4m06nv$gk6@sol.sun.csd.unb.ca> <31883809.2748@whale.st.usm.edu> <319CBF82.1C01@umnw.ethz.ch> NNTP-Posting-Host: lse421-1.microb.okstate.edu X-Posted-From: InterNews 1.0@news.cis.okstate.edu. Xdisclaimer: No attempt was made to authenticate the sender's name. Has anyone had much experience using GelCompar? What are your impressions of this software? Can anyone compare it with Dendron? From owner-rapd@net.bio.net Tue May 21 23:00:00 1996 Path: biosci!KB.USM.MY!bal From: bal@KB.USM.MY (Bal Singh) Newsgroups: bionet.molbio.rapd Subject: (none) Date: 21 May 1996 18:57:08 -0700 Organization: Universiti Sains Malaysia Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe From owner-rapd@net.bio.net Thu May 23 23:00:00 1996 Path: biosci!UOW.EDU.AU!Al_Griskaitis From: Al_Griskaitis@UOW.EDU.AU ("Al Griskaitis") Newsgroups: bionet.molbio.rapd Subject: Cniderian Microsatellites Date: 23 May 1996 18:59:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Fellow RAPDers, Firstly, I hope no one is frustrated with microsatellite queries on this RAPDs platform. But I get the impression that subscribers are au fait with a range of molecular techniques. To business: I'm in the throws of getting microsatellites prepared for the identification of sea anemone clones. While library construction will probably be unavoidable, the more primers at my disposal, the better! So, if anyone out there is (or knows of a group who is) working with cniderian microsatellites please let me know. Does a database of microsatellite primer sequences for various taxa exist? Many Thanks, Al Griskaitis From owner-rapd@net.bio.net Mon May 27 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!howland.reston.ans.net!news-e2a.gnn.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: zevsolltec@aol.com (ZevSolltec) Newsgroups: bionet.molbio.rapd Subject: Re: DNA Fingerprinting Software Date: 28 May 1996 09:51:19 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 16 Sender: root@newsbf02.news.aol.com Message-ID: <4of0cn$94u@newsbf02.news.aol.com> References: <4oev9o$8qr@newsbf02.news.aol.com> Reply-To: zevsolltec@aol.com (ZevSolltec) NNTP-Posting-Host: newsbf02.mail.aol.com M. Massoudi, Please see our web page for information on DENDRON. Zev __________________________________________ Zev Sunleaf Solltech, Inc. Technology Innovation Center Oakdale, IA 52319 USA 319 335-4702 319 335-4489 (fax) Email:ZevSolltec@aol.com http://members.aol.com/ZevSolltec/DENDRON.html __________________________________________ From owner-rapd@net.bio.net Mon May 27 23:00:00 1996 Path: biosci!rutgers!uwm.edu!vixen.cso.uiuc.edu!newsfeed.internetmci.com!in2.uu.net!EU.net!usenet1.news.uk.psi.net!uknet!peer.news.xara.net!xara.net!zynet.net!usenet From: Peter Jackman Newsgroups: bionet.molbio.rapd Subject: Dendron/GelCompar Date: Tue, 28 May 1996 23:02:57 +0100 Organization: BioSystematica Lines: 17 Message-ID: <31AB7811.5929@biosys.zynet.co.uk> NNTP-Posting-Host: biosys.zynet.co.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01Gold (Win95; I) GelCompar also has the feature to construct gels from recombined tracks. Please see our Web site for more details. Dr Peter Jackman BioSystematica ------------------------------------------------------ BIOSYSTEMATICA - the specialists in gel analysis --------------------------------------------------------- 2 Mill Cottages Tel& Fax (+44)-(0)-1822-810827 Peter Tavy Tavistock info@biosys.zynet.co.uk Devon PL19 9AA http://www.cis.plym.ac.uk/biosys/biosys.htm United Kingdom --------------------------------------------------------- From owner-rapd@net.bio.net Mon May 27 23:00:00 1996 Path: biosci!rutgers!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!howland.reston.ans.net!news-e2a.gnn.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: mmassoudi@aol.com (MMASSOUDI) Newsgroups: bionet.molbio.rapd Subject: DNA Fingerprinting Software Date: 28 May 1996 09:32:40 -0400 Organization: America Online, Inc. (1-800-827-6364) Lines: 8 Sender: root@newsbf02.news.aol.com Message-ID: <4oev9o$8qr@newsbf02.news.aol.com> Reply-To: mmassoudi@aol.com (MMASSOUDI) NNTP-Posting-Host: newsbf02.mail.aol.com Dear Netters I am looking for relatively inexpensive (shareware is preferred) PC or Mac based DNA fingerprinting software programs for accurate fragment sizing and matching. The program should be able to convert the saved data for various statistical analysis such as relatedness or similarity index. I do appreciate your suggestions. M. Massoudi From owner-rapd@net.bio.net Tue May 28 23:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!in1.uu.net!EU.net!sun4nl!sci.kun.nl!usenet From: Joop Ouborg Newsgroups: bionet.molbio.rapd Subject: stratiotes rapd Date: Wed, 29 May 1996 18:22:26 +0200 Organization: University of Nijmegen, The Netherlands Lines: 8 Message-ID: <31AC79C2.722F@sci.kun.nl> NNTP-Posting-Host: mbiol9.sci.kun.nl Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Win16; I) At the moment we are working here on RAPD analysis of sexual and asexual Stratiotes aloides populations. Untill now we have had a lot of problems, apparently with lots of secundary compounds in our extraction but also with other less defined problems. Does anyone out there has any experience with DNA extraction and/or RAPD analysis of Stratiotes (or ant other aquatic macrophytes). Please let us know. From owner-rapd@net.bio.net Wed May 29 23:00:00 1996 Path: biosci!dial.pipex.com!cc08 From: cc08@dial.pipex.com (Debra Whitehead) Newsgroups: bionet.molbio.rapd Subject: MOLECULAR PLANT PATHOLOGY ON-LINE Date: 30 May 1996 09:56:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 75 Sender: daemon@net.bio.net Distribution: world Message-ID: <31ADD2F9.672D@dial.pipex.com> NNTP-Posting-Host: net.bio.net British Society for Plant Pathology MOLECULAR PLANT PATHOLOGY ON-LINE The British Society for Plant Pathology (BSPP) has developed an exciting new way to publish research in molecular plant pathology and nematology - the electronic journal Molecular Plant Pathology On-Line (MPPOL). MPPOL is: - Peer-reviewed - through BSPP - Fast - saving the months normally taken to print, bind and distribute on paper - Accessible - at the desk or bench, straight from the Internet - Convenient - search, browse, view, print or download, any time - Free - BSPP bears the modest costs, as a service to international plant pathology - Versatile - colour illustrations are encouraged; movies and sound bites are welcome What you need to use MPPOL: - A PC, Mac or Workstation connected to the Internet - even connect at home by telephone - A World Wide Web browser - e.g. Netscape, MS Explorer, Mosaic Start now at http://www.bspp.org.uk/mppol. You will find: - Full details of MPPOL - International list of editors - Information for authors To submit a paper - E-mail your script to us (or send it on disk) * - You receive an acknowledgement by e-mail - Your script is assigned to an editor - Peer-review starts, by e-mail - Your editor and you correspond by e-mail - Your editor recommends the agreed script to the Senior Editors - The Senior Editors confer, and your script is published in MPPOL * Use the specified format (http://www.bspp.org.uk/mppol/prospect.htm) E-mail address: cc08@dial.pipex.com. Postal address: Dr Debra Whitehead, MPPOL, The Royal Horticultural Society's Garden, Wisley, Woking, Surrey GU23 6QB, UK. Respond quickly to the review, and help us meet our target: - 7 WEEKS FROM RECEIPT OF SCRIPT TO REACHING YOUR READERS Abbreviated list of Editors of MPPOL: - Dr John Antoniw, Dr James Brown, Dr David Cooke, Dr Matt Dickinson, Professor Adrian Gibbs, Dr Henriette Giese, Professor Zhou GuangHe, Dr C. Hiruki, Dr Wolfgang Knogge, Dr Joe McDermott, Dr Bruce McDonald, Dr Adrian Newton (Senior Editor), Dr Kevin O'Donnell, Dr Anne Osbourn, Professor Tapio Palva, Dr Tom Powers, Dr Peter Scott (Senior Editor), Dr Ichiro Uyeda, Professor Mike Wilson, Dr Debra Whitehead (Senior Editor). More details about MPPOL or BSPP? - Check our World Wide Web site at http://www.bspp.org.uk - Contact the BSPP Membership Secretary: Dr Adrian Newton Scottish Crop Research Institute Invergowrie, Dundee DD2 5DA, Scotland, UK E-mail: a.newton@scri.sari.ac.uk Fax: +44 (0)1382 562426 Thank you for reading this message from the BRITISH SOCIETY FOR PLANT PATHOLOGY