From owner-rapd@net.bio.net Thu Nov 07 22:00:00 1996 Path: biosci!mg.sdstate.edu!haleys From: haleys@mg.sdstate.edu ("Haley, Scott Pl.Sci") Newsgroups: bionet.molbio.rapd Subject: ???? Date: 8 Nov 1996 07:21:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <32834FC7@msmail.sdstate.edu> NNTP-Posting-Host: net.bio.net Is there no moderator for this newsgroup? This stuff is crap. ---------- From: Str8.Man To: rapd Subject: @@@>> HOT COLLEGE BI-MALES!!! >PRIVATE< 1-900-825-6000 xt 9794 Date: Sunday, November 03, 1996 3:32PM HOT COLLEGE STR8, BI, AND GAY GUYS NEEDED FOR FRIENDS AND/OR RELATIONSHIPS! ALL AREAS! NOWHERE TO BIG OR SMALL FOR THE LOCATOR! ALL PRIVATE! ALL CONFIDENTIAL! ALL AGES! LADIES WELCOME! THE LOCATOR 1 - 9 0 0 - 8 2 5 - 6 0 0 0 xt 9 7 9 4 1 - 9 0 0 - 8 2 5 - 6 0 0 0 xt 9 7 9 4 1 - 9 0 0 - 8 2 5 - 6 0 0 0 xt 9 7 9 4 2.99/min From owner-rapd@net.bio.net Thu Nov 07 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Wolfgang Wuster Newsgroups: bionet.molbio.rapd Subject: Re: ???? Date: 8 Nov 1996 17:46:14 -0000 Lines: 40 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <55vrl6$t2a@mserv1.dl.ac.uk> X-Sender: bss166@hysterix MIME-Version: 1.0 Original-To: "Haley, Scott Pl.Sci" On 8 Nov 1996, Haley, Scott Pl.Sci wrote: > > Is there no moderator for this newsgroup? This stuff is crap. > Thanks for finding out for us and letting us know. It'll save us some phone calls ;-) > ---------- > From: Str8.Man > To: rapd > Subject: @@@>> HOT COLLEGE BI-MALES!!! >PRIVATE< 1-900-825-6000 xt 9794 > Date: Sunday, November 03, 1996 3:32PM > > > HOT COLLEGE STR8, BI, AND GAY GUYS NEEDED FOR FRIENDS AND/OR > RELATIONSHIPS! > > ALL AREAS! NOWHERE TO BIG OR SMALL FOR THE LOCATOR! > ALL PRIVATE! > ALL CONFIDENTIAL! > ALL AGES! > LADIES WELCOME! > > THE LOCATOR > 1 - 9 0 0 - 8 2 5 - 6 0 0 0 xt 9 7 9 4 > 1 - 9 0 0 - 8 2 5 - 6 0 0 0 xt 9 7 9 4 > 1 - 9 0 0 - 8 2 5 - 6 0 0 0 xt 9 7 9 4 > > 2.99/min > -- Wolfgang Wuster School of Biological Sciences, University of Wales, Bangor, UK e-mail: w.wuster@bangor.ac.uk Thought for the day: If you see a light at the end of the tunnel, it is probably a train coming your way. From owner-rapd@net.bio.net Fri Nov 08 22:00:00 1996 Path: biosci!bioag.byu.edu!JLFarmer From: JLFarmer@bioag.byu.edu ("James L. Farmer") Newsgroups: bionet.molbio.rapd Subject: moderator Date: 8 Nov 1996 20:42:03 -0800 Organization: BYU - Biology and Agriculture Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <7950D2F2F@adena.byu.edu> NNTP-Posting-Host: net.bio.net For those of you who are new to the rapd newsgroup, we have discussed the possibility of a moderator in the past. The decision then was that the convenience of immediate responses outweighed the inconvenience of commercial postings. I know of no reason to change that decision. However, anyone is free to renew the discussion, since we have no moderator. [For informational purposes, I started the discussion group, but I would not be willing to be a moderator because I do not have the time. If we decide to have a moderator, I would be pleased to turn my meager duties over to a volunteer.] James L. Farmer Department of Zoology Brigham Young University Provo, Utah 84602, USA 801-378-2153 (office) 801-378-7423 (FAX) JLFarmer@bioag.byu.edu From owner-rapd@net.bio.net Sun Nov 10 22:00:00 1996 Path: biosci!rutgers!gatech!csulb.edu!newshub.csu.net!www.nntp.primenet.com!nntp.primenet.com!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!cpk-news-feed4.bbnplanet.com!news.fsu.edu!fn3.freenet.tlh.fl.us!usenet From: "mehboob b.sheikh" Newsgroups: bionet.jobs,bionet.molbio.proteins,bionet.molbio.rapd,bionet.plants Subject: Post-doc/visiting scientist position available Date: Mon, 11 Nov 1996 17:45:29 -0500 Organization: FAMU Lines: 25 Message-ID: <3287AC88.5231@freenet3.scri.fsu.edu> NNTP-Posting-Host: fts4p8-bfs.scri.fsu.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win95; I) Xref: biosci bionet.molbio.proteins:9246 bionet.molbio.rapd:1710 bionet.plants:13225 POSITION ANNOUNCEMENT Research Associate - Molecular Biology. Two postdoctoral positions are available to work on peanut molecular biology. The successful candidates will conduct research on the molecular biology of drought and disease resistance, and improving protein quality of peanut. Major goals will include identifying molecular markers for disease and drought resistance, cloning methionine-rich protein gene, and transformation of peanut. The appointee will be expected to have demonstrated experience in modern molecular biology techniques, which may include mRNA isolation and in vitro translation, southern and northern hybridization, RT-PCR, gene cloning/vector construction, screening cDNA library using polyclonal antibodies, DNA sequencing and related techniques, to detect, describe and manage the behavior of the genes. The laboratory is fully equipped with necessary instrumentation and supplies for the above research. A Ph.D. in plant science with extensive knowledge and experience in plant molecular biology is required. Applicants must submit a letter of application describing research and career interests and experience, resume, transcripts and three references to Dr. Mehboob B. Sheikh, Plant Biotechnology Program, Division of Agricultural Sciences, Florida A&M University, Tallahassee, Florida 32307-4100. Phone: (904) 561-2218; E-Mail: Sheikh1@juno.com. Florida A&M University is an Equal Opportunity/Affirmative Action Employer. Minority candidates and women are encouraged to apply. From owner-rapd@net.bio.net Tue Nov 12 22:00:00 1996 Newsgroups: bionet.molbio.rapd,bionet.molbio.yeast Path: biosci!rutgers!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!newsfeed.internetmci.com!malgudi.oar.net!utnetw!mabrini From: mabrini@uoft03.utoledo.edu (Pan Parag) Subject: Look here! Message-ID: Sender: news@utnetw.utoledo.edu (News Manager) X-Newsreader: TIN [version 1.2 PL2] Date: Tue, 12 Nov 1996 22:41:38 GMT Lines: 15 Xref: biosci bionet.molbio.rapd:1711 bionet.molbio.yeast:6105 I am looking for anybody who is a practicing biologist or a biology research professional who can grant me a 15 minute interview preferably over the phone. I am doing this as part of my assignment for my Biology class so that I can graduate. If anybody is willing to help me out, please respond with your name and phone number to the following e-mail address: mabrini@uoft03.utoledo.edu. If my paper is successful, I will send you a copy of it in the mail. Mark Abrinica University of Toledo 419-472-2999 From owner-rapd@net.bio.net Thu Nov 14 22:00:00 1996 Path: biosci!NS1.FAMU.EDU!JLU From: JLU@NS1.FAMU.EDU Newsgroups: bionet.molbio.rapd Subject: (none) Date: 14 Nov 1996 17:46:34 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611150145.RAA09782@net.bio.net> NNTP-Posting-Host: net.bio.net Please subscribe me. From owner-rapd@net.bio.net Sat Nov 16 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Nov 1996 02:00:41 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611171000.CAA09416@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-rapd@net.bio.net Mon Nov 18 22:00:00 1996 Path: biosci!bioag.byu.edu!JLFarmer From: JLFarmer@bioag.byu.edu ("James L. Farmer") Newsgroups: bionet.molbio.rapd Subject: (Fwd) E-Mail Virus Warning Date: 19 Nov 1996 12:09:39 -0800 Organization: BYU - Biology and Agriculture Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <44FDB20D7@adena.byu.edu> NNTP-Posting-Host: net.bio.net Roland Koelliker forwarded an e-mail virus warning to this news group (I have reproduced only a small portion of it for purpose of identification only). I sent a copy to our postmaster asking if there is such a virus. The postmaster's response was "This is another false alarm. Thanks for checking." I post this for your information. I suggest that you not forward the original message, which seems to be a virus of a different sort-- one that clogs up the internet. I am sure that Dr. Koelliker is not responsible for the origination of this message. He is, as I have been before him, an innocent victim. Best wishes. James L. Farmer jlfarmer@byu.edu ------- Forwarded Message Follows ------- To: rapd@net.bio.net From: Roland Koelliker Subject: E-Mail Virus Warning Date: 19 Nov 1996 06:31:46 -0000 >> virus warning >> >> **** SUBJECT: New and Dangerous 'E-mail' Virus - For your >>>> information... >>>> >>>> Please pass on this information to your colleagues. >>>>.... From owner-rapd@net.bio.net Mon Nov 18 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!news.sprintlink.net!news-peer.sprintlink.net!howland.erols.net!newsfeed.internetmci.com!peerfeed.internetmci.com!newsfeed.dacom.co.kr!arclight.uoregon.edu!news.bc.net!nntp.mbnet.mb.ca!canopus.cc.umanitoba.ca!news From: Brian Fristensky Newsgroups: bionet.molbio.rapd Subject: Re: E-Mail Virus Warning Date: Tue, 19 Nov 1996 14:58:39 -0600 Organization: University of Manitoba Lines: 35 Message-ID: <32921F7F.6586@cc.umanitoba.ca> References: <56rk8i$k84@mserv1.dl.ac.uk> NNTP-Posting-Host: brassica.cc.umanitoba.ca Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; SunOS 5.5 sun4u) To: Roland Koelliker Roland Koelliker wrote: > > >> virus warning > >> > >> **** SUBJECT: New and Dangerous 'E-mail' Virus - For your > >>>> information... > >>>> > >>>> Please pass on this information to your colleagues. > >>>> > >>>> This is NOT a joke... > >>> > >>> There is a computer virus that is being sent across the > >>>> Internet. If you receive an e-mail message with the subject line > >>>> "Good Times", DO NOT read the message, DELETE it NO NO NO! This is a hoax that was circulated by email about two years ago. Apparently, somebody thought it was time to start it up again. There is not and never was a Good Times virus, except in the sense that the message itself is a virus that replicates because people think that the message is legitimate, and send it on to others. To the best of my knowledge, there is no way to write a virus that can be activated simply by reading an email message. =============================================================================== Brian Fristensky | Department of Plant Science | There are more elk than people in Wallowa University of Manitoba | County. The people like it that way. Winnipeg, MB R3T 2N2 CANADA | So, most likely do the elk. frist@cc.umanitoba.ca | Office phone: 204-474-6085 | Jonathan Nicholas, 1989 FAX: 204-261-5732 | Plaque in Oregon Convention Center http://home.cc.umanitoba.ca/~frist/ =============================================================================== From owner-rapd@net.bio.net Mon Nov 18 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: Roland Koelliker Newsgroups: bionet.molbio.rapd Subject: E-Mail Virus Warning Date: 19 Nov 1996 06:31:46 -0000 Lines: 77 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <56rk8i$k84@mserv1.dl.ac.uk> X-Sender: rkoellik@patho1 content-length: 3638 Original-To: rapd@dl.ac.uk >> virus warning >> >> **** SUBJECT: New and Dangerous 'E-mail' Virus - For your >>>> information... >>>> >>>> Please pass on this information to your colleagues. >>>> >>>> This is NOT a joke... >>> >>> There is a computer virus that is being sent across the >>>> Internet. If you receive an e-mail message with the subject line >>>> "Good Times", DO NOT read the message, DELETE it >>>> immediately. Please read the messages below. Some >>>> miscreant is sending e-mail under the title "Good Times" >>>> nationwide, if you get anything like this, DON'T DOWNLOAD >>>> THE FILE! It has a virus that rewrites your hard drive, >>>> obliterating anything on it. Please be careful and forward this >>>> mail to anyone you care about. >>>> >>>> The FCC released a warning last Wednesday concerning a >>>> matter of major importance to any regular user of the Internet. >>>> Apparently a new computer virus has been engineered by a >>>> user of AMERICA ON LINE that is unparalleled in its destructive >>>> capability. Other more well-known viruses such as "Stoned", >>>> "Airwolf" and "Michaelangelo" pale in comparison to the >>>> prospects of this newest creation by a warped mentality. What >>>> makes this virus so terrifying, said the FCC, is the fact that no >>>> program needs to be exchanged for a new computer to be >>>> infected. It can be spread through the existing e-mail systems >>>> of the Internet. >>>> >>>> Once a Computer is infected, one of several things can happen. >>>> If the computer contains a hard drive, that will most likely be >>>> destroyed. If the program is not stopped, the computer's >>>> processor will be placed in an nth-complexity infinite binary >>>> loop-which can severely damage the processor if left running >>>> that way too long. >>>> >>>> Unfortunately, most novice computer users will not realize >>>> what is happening until it is far too late. Luckily, there is one >>>> sure means of detecting what is now known as the "Good >>>> Times" virus. It always travels to new computers the same way >>>> in a text e-mail message with the subject line reading "Good >>>> Times". Avoiding infection is easy once the file has been >>>> received simply by NOT READING IT! The act of loading the file >>>> into the mail server's ASCII buffer causes the "Good Times" >>>> mainline program to initialize and execute. >>>> >>>> The program is highly intelligent-it will send copies of itself >>>> to everyone whose e-mail address is contained in a receive-mail >>>> file or a sent-mail file, if it can find one. It will then >>>> proceed to trash the computer it is running on. >>>> >>>> The bottom line is: -if you receive a file with the subject line >>>> "Good Times", delete it immediately! Do not read it!! Rest >>>> assured that whoever's name was on the "From" line was >>>> surely struck by the virus. Warn your friends and local system >>>> users of this newest threat to the Internet! It could save them a >>>> lot of time and money. >>>> >>>> Could you pass this along to your global mailing list as >>>> well? >> >> >> >> As you can see this is not a pretty virus. > Roland Koelliker Swiss Fed Inst Technol Plant Sciences ETH-Zentrum, LFW-C47 CH-8092 Zurich Tel ++41-1-632 3888 Fax ++41-1-632 1153 e-mail: koelliker@ipw.agrl.ethz.ch From owner-rapd@net.bio.net Sat Nov 23 22:00:00 1996 Path: biosci!OMRB.PNPI.SPB.RU!tretyakov From: tretyakov@OMRB.PNPI.SPB.RU ("Alexander N. Tretyakov") Newsgroups: bionet.molbio.rapd Subject: You can make microplates by your cycler Date: 24 Nov 1996 04:04:42 -0800 Organization: BIOD PNPI Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <3298E04D.10A2@omrb.pnpi.spb.ru> NNTP-Posting-Host: net.bio.net Hi All You can modify yuor heat block cycler to make ultra-thin walled microplates out of polymer films. ( Tretyakov et al. Rapid heat block temperature cycling system automatically molding ultra - thin wall microtitre plates CSHL Meet. 9/9-13/9 1996 ). The principle is very simple. It loks like vacuum-blotting of the polypropylene film onto the overheated thermal block of the cycler. The microplate transfer heat to the samples practically immediatelly. It is 100-1000 times cheaper than commercial. I use 96-well microplate ( 4x6 cm ) with 4.5 mm between well spacing (for multichannel pipett). The well-vol. is 30 mcl. You may use mineral oil or heated lid for sealing. As duaring cycling the microplate the microplate looks like "polymer skin" of the block (tightly attached by vacuum ) you cannot destroy it by pressing the lid. Though I designed it for my cycler operating at 7 grad.C/ sec ( Tretyakov et al. Rapid DNA amplification in pipettor tips. Mol Biol- Engl Tr 28/3 part2 p.441-443 1994 ) the method may be usefull for slow commercial heat block cyclers. We make RAPDs since 1989 (under the name universal primer-PCR )-it can be done in 15-30 min. P.S. Recyclabe polypropylen ( PP ) film you can bye in the nearest shop (various packages for papers ) Good Luck Alexander From owner-rapd@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!news-peer.gsl.net!news.gsl.net!news-dc.gsl.net!news.gsl.net!news.NetVision.net.il!news From: Steven Lemore Newsgroups: bionet.molbio.rapd Subject: pH Measurement of Small Biological Samples Date: 26 Nov 1996 19:41:02 GMT Organization: NetVision LTD. Lines: 14 Message-ID: <57fh4e$b1s@news.NetVision.net.il> NNTP-Posting-Host: ts001p10.pop2a.netvision.net.il Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) The need frequently arrises in biological laboratories to measure pH in small sample vessels such as 96 well plates, microcentrifuge tubes, or NMR tubes. Standard pH electrodes are too large to fit into these small containers. Miniature glass electrodes are both breakable and have a high electrical resistance which makes the pH measurements unstable. A micro combination pH electrode has been developed which has a 1 mm tip and a 2 mm diameter flexible Teflon body which can measure pH in less less than 20 microliters of sample. This probe has a solid state sensor which gives drift free pH measurements. The probe can be used with a standard pH meter. Further information can be obtained via email at service@lazarlab.com From owner-rapd@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!rutgers!uwm.edu!cs.utexas.edu!howland.erols.net!news.sprintlink.net!news-peer.sprintlink.net!visi.com!mr.net!newshub.tc.umn.edu!alpha.mrs.umn.edu!markvo@cda.mrs.umn.edu From: "Christopher T. Cole" Newsgroups: bionet.molbio.rapd Subject: Re: Molecular Biology Pioneers Date: Tue, 26 Nov 96 14:18:29 CDT Organization: University of Minnesota - Morris Lines: 26 Message-ID: <63568.colect@caa.mrs.umn.edu> Reply-To: NNTP-Posting-Host: sci242-cc.mrs.umn.edu X-Minuet-Version: Minuet1.0_Beta_17A X-POPMail-Charset: English To: nthomas@mercury.calstatela.edu Nigel-- essential reading on this topic is Horace Judson's _The Eighth Day of Creation_. Outstanding writing, excellent collective biography and history of science. You'll love it. -Chris Cole On 26 Nov 1996 11:17:02 GMT, Nigel J. Thomas wrote: >I am looking for biographical information on Molecular Biology pioneers >Maurice Wilkins and Max Perutz, known for their work on the X-ray >crystallography of DNA and haemoglobin respectively. In particular, can >anyone tell me if (or where I might look to find out if) either of them are >still alive, and, if not, when and where did they die. However, any other >biographical information (preferably with references) or sugestions as to >where I might be able to look it up would be appreciated. > >Please reply by email as my newsgroup connection is a bit unreliable, and >very clunky.. > >Thanks, >Nigel > From owner-rapd@net.bio.net Mon Nov 25 22:00:00 1996 Path: biosci!rutgers!uwm.edu!news-peer.gsl.net!news.gsl.net!howland.erols.net!usc!newshub.csu.net!mercury!nthomas From: nthomas@mercury.calstatela.edu (Nigel J. Thomas) Newsgroups: bionet.molbio.rapd Subject: Molecular Biology Pioneers Date: 26 Nov 1996 11:17:02 GMT Organization: Information Resources and Technology Lines: 13 Message-ID: <57ejje$9ug@nuke.csu.net> NNTP-Posting-Host: mercury.calstatela.edu I am looking for biographical information on Molecular Biology pioneers Maurice Wilkins and Max Perutz, known for their work on the X-ray crystallography of DNA and haemoglobin respectively. In particular, can anyone tell me if (or where I might look to find out if) either of them are still alive, and, if not, when and where did they die. However, any other biographical information (preferably with references) or sugestions as to where I might be able to look it up would be appreciated. Please reply by email as my newsgroup connection is a bit unreliable, and very clunky.. Thanks, Nigel From owner-rapd@net.bio.net Tue Nov 26 22:00:00 1996 Path: biosci!UNIXG.UBC.CA!hobbs From: hobbs@UNIXG.UBC.CA (John Hobbs) Newsgroups: bionet.molbio.rapd Subject: Insect nuclear primer set. Date: 27 Nov 1996 10:59:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 457 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Insect molecular phylogenists who use RAPD but do not subscribe to bug-net may be interested to know that we have an insect nuclear primer set available: details are given in the posting below. Our 10-mer primer sets for RAPD, our insect mitochondrial DNA primer set, our DDRT-PCR kit and our SSR-directed primer set (Set #9) continue to be available. Please contact me if you require details. John Hobbs. **************************************************************************** We are happy to announce the availability of the insect nuclear DNA primer set that has been discussed on bug-net for some time. The set is described below. and orders should be sent to: Dr. John Hobbs EMAIL hobbs@unixg.ubc.ca Nucleic Acid - Protein Service (NAPS) Unit Biotechnology Laboratory Room 237, Wesbrook Building 6174 University Boulevard University of British Columbia Vancouver, V6T 1Z3 Canada. FAX (604)822-5437 ; Tel. (604)822-6373 This primer set was compiled by B. Crespi, with contributions and assistance from M. Berbee, A. Brower, D. Carmean, R. DeSalle, B. Farrell, T. Friedlander, B. Normark, S. Palumbi, J. Regier, H. Robertson, G. Roderick, and C. Simon. Dr. Crespi would especially like to thank Andy Brower and Ben Normark for making available the sequences for unpublished primers that they have designed and tested. The purpose of the compilation is to make easily available a set of primers for nuclear genes for insect systematics and population biology. These primers were chosen because they are known, expected, or hoped to work with more than one order of insects. The contributors should be considered in no way responsible for multiple copies, recombination, huge introns, lineage sorting, lack of amplification in your favourite insects, or amplification of your pet iguana by mistake. Remember: working with nuclear DNA sequence is considerably more complex than working with mtDNA sequence. Please see the references associated with (most of) the primers for details concerning primer design and usefulness. All primers are listed 5' to 3'. ______________________________ NUCLEAR PRIMER SET ______________________________ (1) Protein-coding genes: ELONGATION FACTOR-1ALPHA Cho, Mitchell, Regier, Mitter, Poole, Friedlander and Zhao, 1995. A highly conserved nuclear gene for low-level phylogenetics: Elongation factor - 1alpha recovers morphology - based tree for heliothine moths. Mol. Biol. Evol. 12:650-656, 1995. M3 CACAT(CT)AACATTGTCGT(CG)AT(CT)GG [2103] rcM44.9 CTTGATGAAATC(CT)CTGTGTCC [2342] M44-1 GCTGAGCG(CT)GA(GA)CGTGGTATCAC [2277] rcM51-1 CAT(GA)TTGTC(GT)CCGTGCCA(GT)CC [2645] M46-1 GAGGAAAT(CT)AA(GA)AAGGAAG [2582] rcM52.6 GC(CT)TCGTGGTGCAT(CT)TC(GC)AC [2936] M51.9 CA(GA)GACGTATACAAAATCGG [2832] rcM53-2 GCAATGTG(GA)GCIGTGTGGCA [3149] M52.7 C GTCAAGGA(GA)(CT)TGCGTCGTGG [3030] rcM4 ACAGC(CGA)AC(GT)GT(TC)TG(CT)CTCAT(AG)TC [3344] Degenerate positions are identified by placing the appropriate nucleotides within parentheses. "rc" stands for "reverse complement" and indicates that the primer binds to the sense strand of the DNA. Numbers in brackets at the 3' end of each primer refer to nucleotide position relative to the Drosophila melanogaster sequence. I, inosine. These primers work in some Lepidoptera, and possibly in many other insects and arthropods. ***** Primers designed and submitted by Dr. Ben Normark, Department of Ecology and Evolution, University of Arizona, Tucson: EFS599 Elongation factor 1-alpha (23-mer) ATCTCCGGATGGCACGGYGACAA Fly (F1 copy) ..T..............C..... Fly (F2 copy) .................C..... Bee .................C..... Moth (Bombyx) ..T..T...........A..... Brine shrimp ..T..T........T..T..... EFA923 Elongation factor 1-alpha (22-mer) ACGTTCTTCACGTTGAARCCAA Fly (F1 copy) ........G........G.... Fly (F2 copy) .................G.... Bee .T...............G.... Moth (Bombyx) ........T........A..T. Brine shrimp .....T..G..A.....A.... Designed by B. Normark on the basis of fly, bee, and brine shrimp. Successfully amplifies beetle (weevil genus Aramigus) elongation factor 1a. Attempts to amplify aphid genes failed. In conjunction with EFS599, amplifies a fragment containing an intron whose position is conserved between bee and fly F2. This fragment also contains an intron (in a slightly different position) in weevils. ***** PHOSPHOENOLPYRUVATE CARBOXYKINASE (Friedlander,Regier, Mitter and Wagner. 1995. A nuclear gene for higher level phylogenetics: Phosphoenolpyruvate carboxykinase tracks Mesozoic-age divergences within Lepidoptera (Insecta). Mol Biol Evol 13:594-604. 284dF GAG GGC TGG CTR GCM GAR CAY ATG (901) 18.5dF TGT GGN AAR ACC AAY YTG GCC ATG (991) 19.5dF GGN GAY GAY ATI GCB TGG ATG (1051) 20.5dF GGI GTI TGG TGG GAR GGI ATG G (1226) 21dNrc CAI AAY CTI GAR TTI GGR TGN GC (1305) 511drc GGM CGC ATT GCR AAY GGR TCR TGC AT (1532) 22.5drc GAA CCA RTT RAC RTG RAA GAT C (1630) Primer name abbreviations: d = degenerate, F = forward, N = new version, rc = reverse complement. Non-standard nucleotide abbreviations: B = C/G/T, M = A/C, N = A/C/G/T, R = A/G, Y = C/T, I = inosine. Numbers in parentheses following the primer sequences correspond to the 3' end of the primer as localized in the Drosophila melanogaster sequence (GenBank accession no. Y00402). According to Friedlander et al. 1996, these primers, or a subset thereof, work in some Lepidoptera, Trichoptera, Diptera, Siphonaptera, and Mecoptera. Since they were designed from Drosophila, chicken and rat, they should work in other insects as well. ***** GLUCOSE 6 PHOSPHATE DEHYDROGENASE Soto-Adames, F., H. M. Robertson and S. H. Berlocher. 1994. Phylogenetic utility of partial DNA dequences of G6pdh at different taxonomic levels in Hexapoda with emphasis on Diptera. Ann. Entomol. Soc. Amer. 87:723-736 G6PDH 56F: AAR AAR AAR ATH TAY CCN AC G6PDH 209R: TTY TGN ACC ATY TCY TTN CC N= AGCT H= ACT R= AG Y= TC Designed from human and Drosophila. Works in flies, lepidopterans, thysanurans, collembolans only when cDNA is used as the PCR template, though genomic DNA sometimes amplifies in some flies and collembolans. ****** Primers designed and submitted by Dr. Andy Brower, American Museum of Natural History, New York (brower@amnh.org), with modification by Dana Campbell, Harvard University. Wingless: LEPWG1: GAR TGY AAR TGY CAY GGY ATG TCT GG ModLEPWG2: ACT ICG CAR CAC CAR TGG AAT GTR CA Works for Lepidoptera and Diptera, success reported with crickets. Amplified bit 400 (in leps) to 700 bp (in flies) long. There are 2 large data sets (70+ taxa), for nymphalid (Brower, AMNH) and lycaenid (N. Pierce et al., Harvard U.) butterflies, that have been generated using these primers. The sequences are informative at the intergeneric to subfamily level in these groups. ***** Decapentaplegic: LepDecaA: CGI IGA CTT CGC IGA CGT IGG CTG LepDecaB: CCA CAI CCI ACI ACI ATC ATG TCC TGG TA Designed from three Lepidoptera. Not yet tested thoroughly on other insects. This marker is not recommended as highly as wingless by Brower. **** Sodium Pump The primers cover a 1-2kb fragment and were designed by Brian Farrell from sequences on GenBank for Drosophila, leps and Artemia and fleas. The numbers refer topositions on Drosophila melanogaster. Sequences were first published by Lebovitz, R. M., K Takeyasu and D. M. Fambrough. 1989. EMBO J. 8:193-202. These sometime produce multiple bands in beetles, but work well for other taxa mentioned above. We have been able to produce excellent sequences using these primers for Drosophila and Artemia, but not yet for beetles. This gene is highly conserved, and likely useful for higher insect phylogeny (e.g., for flies as reported in Emery et al 1995). There are introns between these primers for some leps. S389(SDPA): 5' CTG TGG AT(C/T) GG(T/A) G(C/A)(T/G) ATT CT 3' A526(SDPC): 5' ACC ATG TT(G/A) TTG AA(C/G) GAT TCC ATG ATC TT 3' A1669: 5' AG(C/T) TCC ATG TAG GCA TTG TTG A 3' S1124: 5' AG CGT ATG GC(C/A) TC(A/G) AAG AAC TG 3' A1103: 5' CA GTT CTT (C/T)GA (G/T)GC CAT ACG CT 3' ___________________________________________ (2) EPIC (exon-primed intron crossing) primers Palumbi, S. 1996. Nucleic Acids II: The Polymerase Chain Reaction. Chapter 7 of Molecular Systematics II, Sinauer Press, pp. 241-246. See this chapter for important details concerning these primers. For these primers, Y=C,T ; R=A,G ; Z=C,G ; S=C,A ; Q=A,T ; M=A,T,C ; D = G,T ; K = G,T (note that both D and K equal G, T); N = all four bases ; X=amino link. Creatine kinase introns: CK6-5': GAC CAC CTC CGA GTC ATC TCZ ATG CK7-3': CAG GTG CTC GTT CCA CAT GAA ARK7-3': GT GCC AAG GTT GGT DGG GCA CK6 with CK7 works in tephritid flies, and CK6 with ARK7 was designed to work in arthopods. ***** Actin introns: ACT I5': GCT GTT TTC CCG TCC ATT GT ACT II3': GTC CTT CTG CCC CAT ACC SAC CAG Works in some insects and spiders. ***** Cytochrome c introns: cytC-C-5': AAG TGT GCY CAR TGC CAC AC cytC-B-3': CAT CTT GGT GCC GGG GAT GTA TTT CTT Works in some Diptera and Lepidoptera, should work in other arthropods. ***** EF1alpha introns: EF0-5': TCC GGA TGG CAY GGC GAG AAY ATG EF1-5': GAC AAC GTT GGC TTC AAC GTG AAG AAC G EF2-3': AT GTG AGC AGT GTG GCA ATC CAA These primers were designed in collaboration with George Roderick. They work in insects, spiders, crustaceans, and gastropods. Pairing of EFO with EF2 generally provides the best results. ***** Beta-tubulin introns: Tub1-5': CAG GCT GGT CAA TGT GGY AAY CA Tub2-3': CC RTG YTC ATC ACT (TG)AT YAC CTC CCA Tub3-5': GAT TTG GAG CCN GGN ACC ATG GA Tub4-3': AT ACG GTC TGG GTA CTC YTC NCG Tub1 and Tub2 do not work well together. Tub1 with Tub4, and Tub3 with Tub4, work in flies, molluscs and sea urchins. Calmodulin introns: Cal-1 GCC GAG CTG CAR GAY ATG ATC AA dros ... ... ... ..G ..C ... ... .. human ..A ... ... ..G ..T ... ... .. Aplysia ..T ..A T.. ..A ..C ... ... .. Cal-2 GT GTC CTT CAT TTT NCK TGC CAT CAT dros .. ... ... ... ... G.G ... ... ... human .. ... ... ... C.. T.T G.. ... ... Aplysia .. ... T.. ... ... C.T ... ... ... These primers, comminicated to BC by Steve Palumbi, were designed by Tom Duda, Universty of Hawaii, and are similar to those developed independently by Peter Holland. Cal-2 is the reverse complement of the coding sequence and anneals at amino acid position 80. Cal-1 anneals at amino acid position 47, and the amplified product without an intron is ca. 90. Introns occur at amino acid position 60 in snails and urchins and insects and mammals. ___________________________________________ (3) Ribosomal genes and ITS ***** 18S primers Vawter, L. 1991. Evolution of blattoid insects and of the small subunit ribosomal gene. PhD diss, University of Michigan, Ann Arbor. 18S-S22: TAA TGA TCC TTC CGC AGG TTC A 18S-A1984: TCC CTG GTT GAT CCT GCC AGT A These primers amplify the 18S gene in virtually all insects tested. ***** ITS primers ITS4: TCC TCC GCT TAT TGA TAT GC ITS5 GGA AGT AAA AGT CGT AAC AAG G These primers amplify a fragment often about 600 bp long and should work in virtually all of life. These primers are from: White, T. J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322 in M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, eds. PCR Protocols, A Guide to Methods and Applications. Academic Press Inc., San Diego, California and their sequence was provided to BC by Mary Berbee and Dave Carmean. Each set contains 10 nmoles each (which is enough for 1 ml of 10uM solution of each primer) of 50 oligos marked inD-1 to inD-50. The complete sequence list for the set, showing concordance between tube marking, conventional designation, and sequence is enclosed with each set. The oligonucleotides were purified by elution with Tris/EDTA buffer through NAP-5 drip columns. Aliquots of 10 nmoles (10-20 microlitres) each were made near the bottom of the tubes and air dried. All manipulations were conducted in a sterile laminar flow hood. We suggest dissolving your oligos in water or buffer as required, dividing them into portions of adequate size for each intended set of experiments, and storing these as stock solutions at -20 C, thawing portions only when needed. Oligonucleotides subjected to repeated freeze-thaw cycles will become degraded. Concordance: Tube Label / Standard Designation / Sequence Tube / Designation / Sequence (5 prime to 3 prime) Designation Name Sequence inDNA 1 M3 CAC AT(CT) AAC ATT GTC GT(GC) AT(CT) GG inDNA 2 rcM44.9 CTT GAT GAA ATC (CT)CT GTG TCC inDNA 3 M44-1 GCT GAG CG(CT) GA(AG) CGT GGT ATC AC inDNA 4 rcM51-1 CAT (AG)TT GTC (GT)CC GTG CCA (GT)CC inDNA 5 M46-1 GAG GAA AT(CT) AA(AG) AAG GAA G inDNA 6 rcM52.6 GC(CT) TCG TGG TGC AT(CT) TC(GC) AC inDNA 7 M51.9 CA(AG) GAC GTA TAC AAA ATC GG inDNA 8 rcM53-2 GCA ATG TG(AG) GCI GTG TGG CA inDNA 9 M52.7 CGT CAA GGA (AG)(CT)T GCG TCG TGG inDNA 10 rcM4 ACA GC(ACG) AC(GT) GT(CT) TG(CT) CTC AT(AG) TC inDNA 11 EFS599 ATC TCC GGA TGG CAC GG(CT) GAC AA inDNA 12 EFA923 ACG TTC TTC ACG TTG AA(AG) CCA A inDNA 13 284dF GAG GGC TGG CT(AG) GC(AC) GA(AG) CA(CT) ATG inDNA 14 18.5dF TGT GG(AGCT) AA(AG) ACC AA(CT) (CT)TG GCC ATG inDNA 15 19.5dF GG(AGCT) GA(CT) GA(CT) ATI GC(CGT) TGG ATG inDNA 16 20.5dF GGI GTI TGG TGG GA(AG) GGI ATG G inDNA 17 21dNrc CAI AA(CT) CTI GA(AG) TTI GG(AG) TG(AGCT) GC inDNA 18 511drc GG(AC) CGC ATT GC(AG) AA(CT) GG(AG) TC(AG) TGC AT inDNA 19 22.5drc GAA CCA (AG)TT (AG)AC (AG)TG (AG)AA GAT C inDNA 20 G6PDH 56F AA(AG) AA(AG) AA(AG) AT(ACT) TA(CT) CC(AGCT) AC inDNA 21 G6PDH 209R TT(CT) TG(AGCT) ACC AT(CT) TC(CT) TT(AGCT) CC inDNA 22 LEPWG1 GA(AG) TG(CT) AA(AG) TG(CT) CA(CT) GG(CT) ATG TCT GG inDNA 23 ModLEPWG2 ACT ICG CA(AG) CAC CA(AG) TGG AAT GT(AG) CA inDNA 24 LepDecaA CGI IGA CTT CGC IGA CGT IGG CTG inDNA 25 LepDecaB CCA CAI CCI ACI ACI ATC ATG TCC TGG TA inDNA 26 CK6-5' GAC CAC CTC CGA GTC ATC TC(GC) ATG inDNA 27 CK7-3' CAG GTG CTC GTT CCA CAT GAA inDNA 28 ARK7-3' GTG CCA AGG TTG GT(GT) GGG CA inDNA 29 ACT I5' GCT GTT TTC CCG TCC ATT GT inDNA 30 ACT II3' GTC CTT CTG CCC CAT ACC (AC)AC CAG inDNA 31 cytC-C-5' AAG TGT GC(CT) CA(AG) TGC CAC AC inDNA 32 cytC-B-3' CAT CTT GGT GCC GGG GAT GTA TTT CTT inDNA 33 EF0-5' TCC GGA TGG CA(CT) GGC GAG AA(CT) ATG inDNA 34 EF1-5' GAC AAC GTT GGC TTC AAC GTG AAG AAC G inDNA 35 EF2-3' ATG TGA GCA GTG TGG CAA TCC AA inDNA 36 Tub1-5' CAG GCT GGT CAA TGT GG(CT) AA(CT) CA inDNA 37 Tub2i-3' CC(AG) TG(CT) TCA TCT TA(GT) AT(CT) ACC TCC CA inDNA 38 Tub3-5' GAT TTG GAG CC(AGCT) GG(AGCT) ACC ATG GA inDNA 39 Tub4-3' ATA CGG TCT GGG TAC TC(CT) TC(AGCT) CG inDNA 40 Cal-1 GCC GAG CTG CA(AG) GA(CT) ATG ATC AA inDNA 41 Cal-2 GTG TCC TTC ATT TT(AGCT) C(GT)T GCC ATC AT inDNA 42 18S-S22 TAA TGA TCC TTC CGC AGG TTC A inDNA 43 18S-A1984 TCC CTG GTT GAT CCT GCC AGT A inDNA 44 ITS4 TCC TCC GCT TAT TGA TAT GC inDNA 45 ITS5 GGA AGT AAA AGT CGT AAC AAG G inDNA 46 S389(SDPA) CTG TGG AT(CT) GG(AT) G(AC)(GT) ATT CT inDNA 47 A526(SDPC) ACC ATG TT(AG) TTG AA(GC) GAT TCC ATG ATC TT inDNA 48 A1669 AG(CT) TCC ATG TAG GCA TTG TTG A inDNA 49 S1124 AGC GTA TGG C(AC)T C(AG)A AGA ACT G inDNA 50 A1103 CAG TTC TT(CT) GA(GT) GCC ATA CGC T The cost of the set is: for Canadian clients Can.$380 plus applicable taxes (GST for Canadian clients, plus PST for B.C. clients, unless you are fortunate enough to be exempt from these nuisances (in which case, please say so!)); or US$300 for others. Carriage costs are added: airmail costs (small packet category) are US$3 for for the US, or US$4 to most parts of the world, while for courier delivery in the US costs an additional US$11 ($8Can. for shipments within Canada). Courier delivery to other parts of the world is also added at the rate charged to us: $16 to the UK, $16 to Western Europe, $17 to Australasia, $17 to the Far East, $25 to China, South America, and Africa (all prices in US$, based on orders of one or two sets [i.e. up to 500g.]). Orders may be sent by fax, mail or e-mail, and should specify your mailing address, the name of the person to be billed (i.e. grant holder), your preference as to shipment via courier or air mail, and either an official Purchase Order Number or your cheque in advance (made out to "University of British Columbia"). We are also able to accept payment by VISA and MasterCard. If an order is phoned in, a copy of the P.O. for our records is still required. ****************************************************************************** My thanks to Bernie for suggesting and stimulating the assembly of this set. I can accept orders by fax, mail or e-mail to the address below. Orders should specify your mailing address, the name of the person to be billed (i.e. grant holder), your preference as to shipment via courier or air mail, and either an official Purchase Order Number or your cheque in advance (made out to "University of British Columbia"). If you request carriage by courier, please note that our couriers do not deliver to box numbers - a full mailing address is required - and also usually request a contact telephone number. If an order is phoned in, a copy of the P.O. for our records is still required. Dr. John Hobbs Nucleic Acid - Protein Service (NAPS) Unit Biotechnology Laboratory Room 237, Wesbrook Building 6174 University Boulevard University of British Columbia Vancouver, V6T 1Z3 Canada. FAX (604)822-5437 or (604)822-0676; Tel. (604)822-6373 Dr. John Hobbs Nucleic Acid - Protein Service (NAPS) Unit Biotechnology Laboratory Room 237, Wesbrook Building 6174 University Boulevard University of British Columbia Vancouver, V6T 1Z3 Canada. FAX (604)822-5437 or (604)822-0676; Tel. (604)822-6373