From owner-rapd@net.bio.net Sat Feb 01 22:00:00 1997 Path: biosci!maclan.mcgill.ca!thomas_bureau From: thomas_bureau@maclan.mcgill.ca (Thomas Bureau) Newsgroups: bionet.molbio.rapd Subject: Two Post-Doc Positions Date: 1 Feb 1997 17:36:09 -0800 Organization: McGill University, Department of Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <32F3EFD1.727A@maclan.mcgill.ca> Reply-To: 1205@sirocco.CC.McGill.CA, Dr.@sirocco.CC.McGill.CA, Penfield@sirocco.CC.McGill.CA, Avenue@sirocco.CC.McGill.CA, Montreal@sirocco.CC.McGill.CA, QC@sirocco.CC.McGill.CA, H3A@sirocco.CC.McGill.CA, 1B1@sirocco.CC.McGill.CA NNTP-Posting-Host: net.bio.net POSTDOCTORAL POSITIONS AVAILABLE Two postdoctoral positions are available immediately to work on the identification and characterization of transposable elements associated with wild-type plant genes. Many plant genes are associated with members of an unusual superfamily of transposable elements called Miniature Inverted-repeat Transposable Elements or MITEs. Possible projects include investigating the role of MITEs and other transposable elements in gene and genome evolution, characterizing the mechanism of MITE mobility, and using MITEs and other transposable elements in designing novel plant genome mapping and gene isolation protocols [Plant Cell 6, 907-916 (1994); PNAS 93, 8524 (1996)]. Candidates must be highly motivated and have a strong background in molecular biology and/or molecular genetics. Expertise in genome mapping or computational biology is desirable but not a prerequisite. Every effort will be made to facilitate the development of future careers in academia or industry. Please send CV, a brief statement of research goals, and the names of three referees to: Dr. Thomas E. Bureau McGill University Department of Biology 1205 Dr. Penfield Avenue Montreal, Quebec H3A 1B1 Canada. Email: thomas_bureau@maclan.mcgill.ca. Montreal is located in the Canadian province of Quebec. It is the second largest French speaking city in the world with Paris, France being the largest. Despite the strong French presence, Montreal is a multicultural multiethnic community. As such there is a great diversity of cultures, festivals, shops, and restaurants. Montreal also provides easy access to wilderness areas in eastern Canada and the northeast US. Housing is very reasonable with an average two bedroom apartment renting for approximately US$500/month. McGill University is a world class institution and one of the top universities in Canada. The Department of Biology is well rounded with faculty specializing in areas of ecology, evolutionary biology, molecular biology, genetics, developmental biology, cell biology, and neurobiology. Plant research is also well supported with a modern Phytotron facility and easy access to experimental field stations. From owner-rapd@net.bio.net Sun Feb 02 22:00:00 1997 Path: biosci!AZUL.CTC.COM.BR!JORGE.CTFT-1.CTFT.CTC From: JORGE.CTFT-1.CTFT.CTC@AZUL.CTC.COM.BR Newsgroups: bionet.molbio.rapd Subject: unsubscribe Date: 3 Feb 1997 03:32:34 -0800 Organization: Centro de Tecnologia Copersucar Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: <8C9667373B@azul.ctc.com.br> NNTP-Posting-Host: net.bio.net unsubscribe ************************************************** Jorge A. G. da Silva E-mail:jorge@azul.ctc.com.br Tel. (019)4298212 ************************************************** From owner-rapd@net.bio.net Wed Feb 05 22:00:00 1997 Path: biosci!vt.edu!fishgen From: fishgen@vt.edu (Bruce J. Turner) Newsgroups: bionet.molbio.rapd Subject: Anolis rapds Date: 6 Feb 1997 08:57:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <199702061657.LAA31319@sable.cc.vt.edu> NNTP-Posting-Host: net.bio.net Anybody out there in rapdland doing rapds on Anolis or related lizards? A grad student in this department is interested in multiple paternity in that group, and she is wondering if rapds are polymorphic enough to do the job, and what general level of experience with lizards exists in the rapd community. Thanks much. Bruce J. Turner VPISU ("Va Tech") Blacksburg, VA 24061-0406 (540)-231-7444 (V) (540)-231-9307 (F) fishgen@vt.edu c.v. and photo: http://art.biol.vt.edu/Facultypages/Turner/Turner2.html From owner-rapd@net.bio.net Sun Feb 09 22:00:00 1997 Path: biosci!UKCC.UKY.EDU!VSC003 From: VSC003@UKCC.UKY.EDU (Ernie Bailey) Newsgroups: bionet.molbio.rapd Subject: Unsubscribe Date: 10 Feb 1997 05:45:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <970210.084603.EST.VSC003@ukcc.uky.edu> NNTP-Posting-Host: net.bio.net Unsubscribe From owner-rapd@net.bio.net Mon Feb 10 22:00:00 1997 Path: biosci!agate!howland.erols.net!newsxfer3.itd.umich.edu!rill.news.pipex.net!pipex!uunet!in2.uu.net!192.174.65.41!01-newsfeed.univie.ac.at!03-newsfeed.univie.ac.at!news.univie.ac.at!news-admin@univie.ac.at From: "Mag. Thomas Mayerhofer" Newsgroups: bionet.molbio.rapd Subject: polysacheride contents in DNA Date: 11 Feb 1997 11:18:34 GMT Organization: Forschungszentrum Seibersdorf Lines: 27 Message-ID: <5dpkia$3faa@www.univie.ac.at> NNTP-Posting-Host: zdla12.arcs.ac.at Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.2N (Windows; I; 16bit) Hi there I've been working with RAPDs for about a year now. Recently I found out, that RAPDs are far more susceptible to minor sugar contamination then other PCR reactions. When a colleague of mine, who is doing the growth experiments and culturing, grew the fungi I'm working on on a new media, the RAPDs didnt work any more. At first I didnt know she's changed the media, but I found out that there were more polysacherides in now. I found a solution to get rid of them, but now the banding patterns are different from the ones before. Also minor polysacheride contents seem to be fine in "normal" PCR they seem to be able to change RAPD banding patterns quite a lot. Has anyone experienced the same before and can tell me ? ---------------------------------------------- Thomas Mayerhofer Abteilung Lebenswissenschaften & Agrarforschung Oesterreicheisches Forschungszentrum Seibersdorf Austrian Research Centre Seibersdorf MAYERHOFER@ARCS.AC.AT ------------------------------------------------- From owner-rapd@net.bio.net Mon Feb 10 22:00:00 1997 Path: biosci!daresbury!not-for-mail From: "K. Eichenberger" (by way of "K. Eichenberger" ) Newsgroups: bionet.molbio.rapd Subject: contamination/DNA content Date: 11 Feb 1997 12:39:58 -0000 Lines: 30 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <5dppau$6rn@mserv1.dl.ac.uk> X-Sender: aerob@rzu-mailhost.unizh.ch Original-To: rapd@dl.ac.uk Hello James L. Farmer wrote about his examinations with the various components of RAPD reactions and their effects to RAPD patterns. I confirm his point of view. I made comparable experiences. Something I want to add is that the adjustement of DNA contents from different extracts has to be considered carefully. I did several experiments with different extracts from the same organ of the same plant (Phaseolus vulgaris, leafs). The different extracts varied after extraction in their DNA contents. I measured these with fluorometry, spectrophotometry and gel electrophoresis (with DNA quantitation markers from Gibco BRL). After adjustement of DNA content based on the three methods I performed RAPDs with all the extracts (12) and compared the patterns. What I found is that spectrophotometry (various types of instruments) in my investigations was not helpful to measure DNA, even to determine relative DNA values. Fluorometry wasn't useful neither. I assume that chlorophyll interfered the measurement. DNA quantification with gel electrophoresis was the most appropriate method. Even here I got few bands that appeared or not apart from a common band pattern. I guess that the different dilutions of the extracts led to different proportions of DNA/PCR interfering components. If I can help, I will give more details. Kurt Eichenberger Rolandstr. 27 8004 Z=FCrich Tel. ..41 1 242 91 65 e-mail aerob@systbot.unizh.ch From owner-rapd@net.bio.net Fri Feb 14 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!battery.awod.com!usenet From: arthurv@awod.com (Ipomoea) Newsgroups: bionet.molbio.rapd Subject: connectix quickcam to grab gel photos? Date: Sat, 15 Feb 1997 20:48:05 GMT Organization: A World of Difference, Inc. Lines: 10 Message-ID: <5e577s$hf7@battery.awod.com> NNTP-Posting-Host: chs0162.awod.com X-Newsreader: Forte Free Agent 1.0.82 This could be a shot in the dark, but for low-budget labs, using Connectix's relatively inexpensive grey scale camera could be a useful tool. There are of course high-end all-in-one workstations that support this application, but we are talking dirt cheap here. Has anybody tried this? Appreciate any info. Arthur From owner-rapd@net.bio.net Sun Feb 16 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Feb 1997 02:00:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199702171000.CAA26629@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-rapd@net.bio.net Tue Feb 18 22:00:00 1997 Path: biosci!ASRR.ARSUSDA.GOV!abartlet From: abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") Newsgroups: bionet.molbio.rapd Subject: Re: RFLPScan for RAPD's Date: 19 Feb 1997 11:58:48 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net David, I have used RFLPscan for about 3 years. I have been quite satisfied with the data produced by the analysis. I have had two problems with the program. When I tried to set boundries on band weights (I have forgotten the term they use in the program - Oh yeah, bins) I found that if I set a low percentage boundry to accomodate small band weights then the variation in large bands produced too many bins. If I set larger limits then too many of the smaller bands were placed in a given bin. I wound up having to do the comparisons by eye which was very time consuming. The second problem was getting the database they use set up to accomodate gels done on different days using a given primer and population. I think that problem may have been that I just wasn't smart enough to make their database work, but it seemed very balky. Oh, I also ended up having to make a 0,1 matrix for all my data by hand after the analysis was completed. I have just purchased a new program from DNA-Proscan (PRO-RFLP) which seems to be much more user friendly. It has some neat tools for lining up identical bands across the gel. It will allow the use of two different standard banding patterns, and it produces a 0,1 matrix as one of the output options. We are just learning to use it, but my test runs show that I get data equivalent to what I was doing by hand in a much shorter time and I think more accurately. If you haven't seen this program, I suggest you take a look at it. (#800-841-4362). Good luck. On Wed, 19 Feb 1997, David Ley wrote: > In a quest for computer-assisted gel analysis software to use for analysis > of RAPD banding patterns and database functions, I am considering > Scanalytics RFLPscan. Testimonials or critiques from users for this or > other applications would be much appreciated. > Thanks, dl. > > Alan C. Bartlett "GENES-R-US" "ALTERATIONS AVAILABLE!^" "^some restrictions may apply:)" From owner-rapd@net.bio.net Tue Feb 18 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!news-relay.ncren.net!nntp-xfer.ncsu.edu!news From: david_ley@ncsu.edu (David Ley) Newsgroups: bionet.molbio.rapd Subject: RFLPScan for RAPD's Date: Wed, 19 Feb 1997 11:28:18 -0500 Organization: NCSU-CVM Lines: 5 Message-ID: NNTP-Posting-Host: faeppc2.cvm.ncsu.edu In a quest for computer-assisted gel analysis software to use for analysis of RAPD banding patterns and database functions, I am considering Scanalytics RFLPscan. Testimonials or critiques from users for this or other applications would be much appreciated. Thanks, dl. From owner-rapd@net.bio.net Tue Feb 18 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!cpk-news-feed1.bbnplanet.com!ox.mc.edu!usenet From: "Robert G. Hamilton" Newsgroups: bionet.molbio.rapd Subject: Re: RFLPScan for RAPD's Date: Wed, 19 Feb 1997 12:40:41 -0500 Organization: Mississippi College Lines: 12 Message-ID: <330B3B19.5C05@csc.mc.edu> References: Reply-To: rhamilto@csc.mc.edu NNTP-Posting-Host: rhamilto.mc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win16; I) David Ley wrote: > > In a quest for computer-assisted gel analysis software to use for analysis > of RAPD banding patterns and database functions, I am considering > Scanalytics RFLPscan. Testimonials or critiques from users for this or > other applications would be much appreciated. > We use RFLPScan, and it works fine in the Windows environment. Students seem to have no problem learning how to use it. I would highly recommend it. ************************************************************************ From owner-rapd@net.bio.net Wed Feb 19 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!www.nntp.primenet.com!nntp.primenet.com!visi.com!news.maxwell.syr.edu!europa.clark.net!worldnet.att.net!howland.erols.net!newsfeed.internetmci.com!forge.sbt.net!news.ia.net!Mournblade.interl.net!usenet From: thoschek@interl.net Newsgroups: bionet.molbio.rapd Subject: LEE'S FRUCTOSE COOK BOOK! Date: 20 Feb 1997 17:48:20 GMT Organization: Interlink L.C. Lines: 9 Message-ID: <5ei2p4$3pd@Mournblade.interl.net> NNTP-Posting-Host: pm2-adr49.interl.net X-Newsreader: SPRY News 3.03 (SPRY, Inc.) lees fructose cook book for healthy eating ____________________________________________________________________________ Tom Hoschek The Gardener's Tea Bag Organic Plant Food email: thoschek@interl.net ____________________________________________________________________________ From owner-rapd@net.bio.net Wed Feb 19 22:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!ox.mc.edu!usenet From: "Robert G. Hamilton" Newsgroups: bionet.molbio.rapd Subject: Re: RFLPScan for RAPD's Date: Wed, 19 Feb 1997 18:56:00 -0500 Organization: Mississippi College Lines: 12 Message-ID: <330B9310.5280@csc.mc.edu> References: Reply-To: rhamilto@csc.mc.edu NNTP-Posting-Host: rhamilto.mc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win16; I) Alan C. Bartlett wrote: > Oh, I also > ended up having to make a 0,1 matrix for all my data by hand after the > analysis was completed. The RFLPScan that I have (v3.0) will make a 0,1 matrix. While I would recommend that anyone check out all options before making a purchase of such software, comparing a 3 year old version of one program to a current version of another is a little unreasonable! I have no affiliation with Scanalytics. From owner-rapd@net.bio.net Fri Feb 21 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!news.bc.net!unixg.ubc.ca!not-for-mail From: "Gary Donaldson" Newsgroups: bionet.molbio.rapd Subject: Re: RFLPScan for RAPD's Date: 22 Feb 1997 00:38:08 GMT Organization: University of British Columbia Lines: 52 Message-ID: <01bc2058$b29ee560$6ade5289@donaldson> References: NNTP-Posting-Host: port06.annex3.net.ubc.ca X-Newsreader: Microsoft Internet News 4.70.1155 I've checked out quite a few of these setups lately for AFLP analysis. Basically the same as RAPDs as far as image analysis goes. I've just seen the demo version of RFLPscan. IMHO, the big pluses with RFLPscan are the database engine, and the ability to format data for use with other widely available programs like PHYLIP, TREECON, and linkage analysis programs. This is nice. But it costs too much. We purchased BioImage's Advanced Quantifier software for Windows, after using a similar product (Whole Band Analyzer) on a UNIX platform. The BioImage software is also too expensive, but was cheaper than what I was quoted for RFLPscan. I have programmed my own database in MS-Access and just export data from BioImage to that for my analyses. I like being able to write the database the way I want it, but I doubt most people would want to bother. Considering the price of these software packages, I was NOT impressed. Both had plenty of bugs, and of course getting those fixed costs extra. And is support included with that large price tag? Hell no, that's extra too. If you have a Mac you use NIH-Image, and export data to a spreadsheet program. With a little tweaking, I'm sure you could setup an analysis system for next to nothing. Sure, not so many bells and whistles, but the price is right. Scion corp. has just released the first beta version of their port of NIH-Image to the PC (it's called ImagePC). Buggy, yes, but also FREE. So, I guess the bottom line is... I think RFLPscan is one of the best thought out applications available. Signal Analytics execution of the idea could have used more polish, and a lower sticker price. Get a demo, if you haven't already, and play with it for awhile. David Ley wrote in article ... > In a quest for computer-assisted gel analysis software to use for analysis > of RAPD banding patterns and database functions, I am considering > Scanalytics RFLPscan. Testimonials or critiques from users for this or > other applications would be much appreciated. > Thanks, dl. > ----------------------------------------------------------------- Gary C. Donaldson Dept. of Plant Science, University of British Columbia Division of Molecular Genetics, B.C. Research Inc. Vancouver, B.C., Canada E-mail: garyd@unixg.ubc.ca OR donaldson@bcr.bc.ca PGP 2.6.2 key available by finger or e-mail request ----------------------------------------------------------------- From owner-rapd@net.bio.net Sun Feb 23 22:00:00 1997 Path: biosci!ASRR.ARSUSDA.GOV!abartlet From: abartlet@ASRR.ARSUSDA.GOV ("Alan C. Bartlett") Newsgroups: bionet.molbio.rapd Subject: Re: RFLPScan for RAPD's Date: 24 Feb 1997 12:50:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <330B9310.5280@csc.mc.edu> NNTP-Posting-Host: net.bio.net A comparison of an older version of a program with a newer program probably is unreasonable unless you consider that Scanalytics has never informed me of a version of their program that would give a 0,1 matrix in spite of my complaints to their representative about this problem. I was really only giving my opinion, not trying to sell either program. Alan C. Bartlett "GENES-R-US" "ALTERATIONS AVAILABLE!^" "^some restrictions may apply:)" From owner-rapd@net.bio.net Tue Feb 25 22:00:00 1997 Path: biosci!ACD.TUSK.EDU!Prakash From: Prakash@ACD.TUSK.EDU ("C. S. Prakash") Newsgroups: bionet.molbio.rapd Subject: African Germplasm: DNA Bank-Net Meeting in Nairobi, Kenya Date: 26 Feb 1997 11:06:48 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net The Third DNA Bank-Net meeting will be held in Nairobi, Kenya during July 20-23, 1997 with focus oon "African Germplasm: Collections, Conservation and Utilization". Scientists in Africa are eligible to receive travel funds to attend this meeting. So Please contact Dr. Robert P. Adams, Baylor University, Box 669, Gruver, TX 79040 USA (Fax 806-733-5605; Email: rpadams@juno.com) immediately. Contact for Local Arrangements: Dr. titus Mukiama, Dept of Botany, University of Nairobi, Chiromo, PO Box 30197, Nairobi, Kenya (Fax: 254-2-216186). If you have friends or colleagues in Africa who can benefit from this meeting or travel grant, please let Dr. Adams know immediately. Do not Write to me!! I am only forwarding this information. C. Prakash \\\\/ ^(@ @)^ --------------------------------oOO--<_>--OOo--------------------------- C. S. Prakash, Ph. D. Prakash@acd.tusk.edu Center for Plant Biotechnology Research Ph: (334) 727 8023 College of Agricultural, Env and Nat Sci. Fax:(334) 727 8552 Tuskegee University http://agriculture.tusk.edu/ Tuskegee, AL 36088, USA -+*%$%*+-+*%$%*+-+*%$%*+-+*%$%*+-+*%$%*+--+*%$%*+-%$%*+-+*%$%*++-+*%$%*+