From owner-rapd@net.bio.net Sun Mar 02 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!news.sgi.com!su-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!ais.net!ameritech.net!uunet!in1.uu.net!204.96.36.2!wizard.pn.com!news.xensei.com!news From: chi@healthtech.com (Cambridge Healthtech Institute) Newsgroups: bionet.molbio.rapd Subject: INFECTION GENOMICS Date: Mon, 03 Mar 1997 14:48:41 GMT Organization: Cambridge Healthtech Institute Lines: 87 Message-ID: <5feo75$g3i@xensei3.xensei.com> Reply-To: chi@healthtech.com NNTP-Posting-Host: chi.xensei.com X-Newsreader: Forte Free Agent 1.0.82 INFECTION GENOMICS APRIL 1-2, 1997 LOEWS CORONADO BAY RESORT, CORONADO, CALIFORNIA Session Chairs Dr. Craig S. Hill, Gen-Probe, Inc. Dr. Stephen A. Johnston, University of Texas Southwestern Medical Center Dr. Peter A. Schad, National Center for Genome Resources Dr. Richard S. Stephens, University of California, Berkeley Additional Speakers Dr. David S. Bailey, Pfizer Central Research Dr. David T. Beattie, Virus Research Institute, Inc. Dr. Patrick Dillon, Human Genome Sciences, Inc. Dr. Ronald W. Ellis, Astra Research Center Boston Dr. Lance Fors, Third Wave Technologies, Inc. Dr. Richard Goold, Incyte Pharmaceuticals, Inc. Dr. Philippe Gros, McGill University Dr. Stephen L. Hoffman, Naval Medical Research Institute Dr. Jörgen Lönngren, ProGene Lab AB Dr. George Natsoulis, Microcide Pharmaceuticals, Inc. Dr. Steven H. Nye, Pharmacia Biotech, Inc. Dr. C. Kendall Stover, PathoGenesis Corporation Dr. William E. Timberlake, ChemGenics Pharmaceuticals Inc. Dr. Jean-François Tomb, The Institute for Genomic Research Dr. Joaquim Trias, Versicor Inc. Dr. Gerald F. Vovis, Genome Therapeutics Corporation Dr. Terry Walker, Becton Dickinson Research Center Dr. C. Richard Wobbe, SCRIPTGEN Pharmaceuticals, Inc. SEQUENCING Comparative Microbial Genomics Using GSDB at NCGR Genomic Expression Analysis in Pathogenic Bacteria PathSeq: Microbial Genome Database The Malaria Genome Project Role of Nramp1 Gene in Resistance to Infectious Diseases Sequencing and Comparative Genomics of Helicobacter Pylori Genome DIAGNOSTICS Infectious Disease Diagnosis Using Strand Displacement Amplification Transcription-Mediated Amplification for Detection of Microorganisms Fingerprinting Viral and Bacterial Genotypes Using CFLP® HIV-1 Drug Resistance Genotyping by DNA-Sequencing APEX as an Analytic Chip-Based Tool for DNA Sequence Variations VACCINES Genetic Vaccines and Genomics Applying Genomic Sequencing and Analysis to H. Pylori Vaccine Microbial Genomics Approach for the Development of Vaccines THERAPEUTICS AND DRUG DEVELOPMENT Treatment and Prevention Approaches Based on Chlamydia Genome Developing Therapeutic Products from Genomic Sequence of Pathogens Drug Discovery Genomics Microbial and Mammalian Genome Sequences to Validate Drug Screening Targets Pathogen Genomic Regions for Infection and Drug Resistance New Antibiotics from Combichem Libraries and Novel Targets Targeted Genomics Approach to Antibacterials and Antifungals Impact of Genomics on the Diagnosis and Treatment of Infectious Disease. For registration and hotel information, please contact: Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 USA Phone: 617-630-1300 Fax: 617-630-1325 e-mail: chi@healthtech.com http://www.healthtech.com/conferences/ ______________________________ Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ tel: 617.630.1300 fax: 617.630.1325 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ inquiries@healthtech.com World Wide Web http://www.healthtech.com/conferences From owner-rapd@net.bio.net Mon Mar 03 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!news.sprintlink.net!news-peer.sprintlink.net!newsfeed.internetmci.com!news.stealth.net!uunet!in1.uu.net!198.151.175.33!news.xensei.com!news From: chi@healthtech.com (Cambridge Healthtech Institute) Newsgroups: bionet.molbio.rapd Subject: Fifth Annual Nucleic Acid-Based Technologies Date: Tue, 04 Mar 1997 15:22:52 GMT Organization: Cambridge Healthtech Institute Lines: 44 Message-ID: <5fhej7$tud@xensei3.xensei.com> Reply-To: chi@healthtech.com NNTP-Posting-Host: chi.xensei.com X-Newsreader: Forte Free Agent 1.0.82 Cambridge Healthtech Institute’s Fifth Annual Nucleic Acid-Based Technologies: New Techniques, Research, and Clinical Applications June 16-17, 1996 The Fairmont Hotel Atop Nob Hill San Francisco, California 94108 Gene amplification and detection has become one of the most widely used techniques for conducting biological research and is being applied to an increasing range of diagnostic and clinical applications. Those researchers working in the technical developments driving this field and those researchers utilizing these new applications are encouraged to submit an abstract detailing their work for consideration of presentation. Session topics include: Noncycling Amplification Gene Quantification In situ Hybridization Disease Diagnosis Human Genotyping If you wish to receive more information, please contact: Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 USA Phone: 617-630-1300 Fax: 617-630-1325 e-mail: chi@healthtech.com http://www.healthtech.com/conferences/ ______________________________ Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ tel: 617.630.1300 fax: 617.630.1325 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ inquiries@healthtech.com World Wide Web http://www.healthtech.com/conferences From owner-rapd@net.bio.net Wed Mar 05 22:00:00 1997 Path: biosci!BIO.VU.NL!wurff From: wurff@BIO.VU.NL ("Drs. A. van der Wurff") Newsgroups: bionet.molbio.rapd Subject: subscribe Date: 6 Mar 1997 06:52:11 -0800 Organization: Animal Ecology (Free University) Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <331EDA4D.714C@bio.vu.nl> Reply-To: wurff@bio.vu.nl NNTP-Posting-Host: net.bio.net please subscribe From owner-rapd@net.bio.net Fri Mar 07 22:00:00 1997 Path: biosci!ACSU.BUFFALO.EDU!coffroth From: coffroth@ACSU.BUFFALO.EDU (Mary-Alice Coffroth) Newsgroups: bionet.molbio.rapd Subject: Manuscript posted Date: 8 Mar 1997 08:03:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net To Fellow -RAPD-netters, A while back I sent out a request for acronyms for molecular techniques. I used these in a talk I was writing on "molecular approaches to the study of clonal organisms". The talk was presented at the 8th International Coral Reef Symposium in Panama last June and the manuscript is in press (in the symposium volume). As there was a page limit for the symposium volume, the paper is not as comprehensive as I would have liked. However, if anyone is interested, I have posted a copy of it on my webpage: http://wings.buffalo.edu/academic/department/fnsm/bio-sci/faculty/reef96.html Again, thanks for all of your help! Cheers, Mary Alice ********************************************* * Mary Alice Coffroth * * Research Assistant Professor * * Department of Biological Sciences * * State University of New York at Buffalo * * Buffalo NY 14260 * * * * Phone: 716-645-2881 * * Fax: 716-645-2975 * * Email: Coffroth@acsu.buffalo.edu * ********************************************* From owner-rapd@net.bio.net Sun Mar 09 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!cs.utexas.edu!howland.erols.net!worldnet.att.net!newsadm From: "M. Massoudi" <"mmassoudi@Worldnet.att.net"@Worldnet.att.net> Newsgroups: bionet.molbio.rapd Subject: Hybrid Testing Date: Mon, 10 Mar 1997 08:56:36 -0800 Organization: Central Valley Seeds Lines: 6 Message-ID: <5g1ehs$omc@mtinsc05.worldnet.att.net> NNTP-Posting-Host: 207.147.205.112 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.01E (Win95; U) To Fellow -RAPD-netters, I am going to use PCR based markers for hybrid testing of pepper and tomato seeds for genetic uniformity and quality control testing. I would like to know if anyone has SSRs, SCARs or specific primers (Co-dominant) for these two crops. Thank you for your suggestions. M. Massoudi From owner-rapd@net.bio.net Tue Mar 11 22:00:00 1997 Path: biosci!DEAKIN.EDU.AU!huangbx From: huangbx@DEAKIN.EDU.AU (Bixing Huang) Newsgroups: bionet.molbio.rapd Subject: Help:FSTAT program Date: 11 Mar 1997 20:50:10 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703120450.PAA08990@hestia.ccs.deakin.edu.au> NNTP-Posting-Host: net.bio.net Dear Netters, I am looking for the program FSTAT used in the paper by Wiard et al (Genetics, 142:1237-1247, 1996), compiled by Goudet (1995). It is not foud on the net. I sould be much appreciated if someone can direct me to where to buy or ftp it. Thanks a lot. With regards Ben Huang huangbx@deakin.edu.au This is a sig From owner-rapd@net.bio.net Tue Mar 11 22:00:00 1997 Path: biosci!deakin.edu.au!huangbx From: huangbx@deakin.edu.au (Bixing Huang) Newsgroups: bionet.molbio.rapd Subject: Help:FSTAT program Date: 11 Mar 1997 18:21:54 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703120219.NAA06066@hestia.ccs.deakin.edu.au> NNTP-Posting-Host: net.bio.net Dear Netters, I am looking for the program FSTAT used in the paper by Wiard et al (Genetics, 142:1237-1247, 1996), compiled by Goudet (1995). It is not foud on the net. I sould be much appreciated if someone can direct me to where to buy or ftp it. Thanks a lot. With regards Ben Huang huangbx@deakin.edu.au From owner-rapd@net.bio.net Wed Mar 12 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news-xfer.netaxs.com!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!newsfeed.internetmci.com!wilbur.sequent.com!newsfeed.orst.edu!news.orst.edu!gus.FSL.ORST.EDU!krutovsk From: krutovsk@fsl.orst.edu (Konstantin Krutovskii) Newsgroups: bionet.molbio.rapd Subject: Re: Help:FSTAT program Date: Wed, 12 Mar 1997 13:46:41 Organization: Forestry Sciences Lab Lines: 80 Distribution: world Message-ID: References: <199703120219.NAA06066@hestia.ccs.deakin.edu.au> NNTP-Posting-Host: gus.fsl.orst.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] In article <199703120219.NAA06066@hestia.ccs.deakin.edu.au> huangbx@deakin.edu.au (Bixing Huang) writes: >From: huangbx@deakin.edu.au (Bixing Huang) >Subject: Help:FSTAT program >Date: 11 Mar 1997 18:21:54 -0800 >Dear Netters, >I am looking for the program FSTAT used in the paper by Wiard et al >(Genetics, 142:1237-1247, 1996), compiled by Goudet (1995). It is not foud >on the net. I sould be much appreciated if someone can direct me to where to >buy or ftp it. >Thanks a lot. >With regards >Ben Huang >huangbx@deakin.edu.au Dear Ben, The following are paragraphs from the FSTAT documentation file kindly provided by Jerome Goudet: Institut de Zoologie et d'Ecologie Animale Bâtiment de Biologie Université de Lausanne CH-1015 Dorigny Switzerland. Phone: +(41 21) 692 24 78 Fax : +(41 21) 692 41 05 E-mail: jerome.goudet@izea.unil.ch How to get FSTAT: 1) If you have an electronic mail that can read binhexed or uuencoded files (such as nupop or pc-eudora), send me an email at the above address, and you will receive the program in a compressed form by return of mail, together with example files. 2) If you have ftp (file Transfer protocol) access, you can download the files after an anonymous login to oracle.bangor.ac.uk ftp oracle.bangor.ac.uk login: anonymous and use your email address as a password. The files reside in the directory pub/fstat, that you access by typing the command: cd pub/fstat The file FSTATDIS.EXE contains all the distribution. It is a self extracting compressed file.It needs downloading in binary format (bin). To receive the files on your computer, enter the command get filename (with extension) when you have received all the files, issue the command quit. To unpack the distribution, copy FSTATDIS.EXE to the directory where you want FSTAT to reside, and issue the command: FSTATDIS For running the protected mode verion, you need additional files, contained in PROMODE.EXE, also a self-extracting compressed file. Once it has been downloaded, uncompress it and read carefully the file DPMIUSER.DOC. The files need to be in the same directory as FSTATP.EXE. 3) As a last resort, and only if 1 and 2 above failed, you could send me a 3 1/2 inch disk, preformated, together with a stamped envelop with your address written on it. ------------------------- Good luck, Kostya krutovskiik@fsl.orst.edu From owner-rapd@net.bio.net Thu Mar 13 22:00:00 1997 Path: biosci!KUCCNX.KOREA.AC.KR!jsshin From: jsshin@KUCCNX.KOREA.AC.KR (Jeong Sheop Shin) Newsgroups: bionet.molbio.rapd Subject: Looking for random 13-mer Date: 14 Mar 1997 02:59:29 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <1.5.4.16.19970314200142.12bfcdcc@kuccnx.korea.ac.kr> NNTP-Posting-Host: net.bio.net I would like to use random 13-mer or longer primer sets for my differetial display experiment. Is anyone aware of e-mail address or fax number of the company dealing with those random primers? I appreciate if you let me know it. Best regards, Jeong Sheop Shin Graduate School of Biotechnology Korea University jsshin@kuccnx.korea.ac.kr From owner-rapd@net.bio.net Thu Mar 13 22:00:00 1997 Path: biosci!OPS1.AGRIC.ZA!CHARLOTTE From: CHARLOTTE@OPS1.AGRIC.ZA ("Charlotte Mienie ", Biochemie) Newsgroups: bionet.molbio.rapd Subject: RAPD Date: 13 Mar 1997 22:42:51 -0800 Organization: AGRICULTURAL RESEARCH COUNCIL Lines: 6 Sender: daemon@net.bio.net Distribution: world Message-ID: <2D207F567E@ops1.agric.za> NNTP-Posting-Host: net.bio.net Hi, Is there anyone with some experience of doing RAPDs on an IDAHO rapidcycler? It uses capillary tubes with a volume of 10microlitres. Charlotte Mienie E-mail:Charlotte@ops1.agric.za From owner-rapd@net.bio.net Thu Mar 13 22:00:00 1997 Path: biosci!HRZ1.UNI-OLDENBURG.DE!grieb From: grieb@HRZ1.UNI-OLDENBURG.DE ("GRIEB GERHARD FB 7") Newsgroups: bionet.molbio.rapd Subject: Resolver GoldTM Date: 14 Mar 1997 02:42:42 -0800 Organization: University Of Oldenburg Comp.Center Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello, is there anyone experienced in using Resolver GoldTM to descriminate between RAPD made fragments of same size but maybe different sequenz? Bye, Gerhard Grieb From owner-rapd@net.bio.net Sun Mar 16 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Mar 1997 02:00:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703171000.CAA14357@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-rapd@net.bio.net Sat Mar 22 22:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsxfer3.itd.umich.edu!feeder.chicago.cic.net!cougar.olivet.edu!usenet From: IRST Newsgroups: bionet.molbio.rapd Subject: IRST Needs your help. Date: Tue, 18 Mar 1997 22:32:44 -0600 Organization: Olivet Nazarene University Lines: 108 Message-ID: <332F6C6C.2CCC@eggbeater.olivet.edu> NNTP-Posting-Host: r330a.chapman.olivet.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.0b2 (Win95; I) X-Priority: 3 (Normal) Dear Internet User, By now you've probably heard about the current issues pertaining to free speech and the regulation of Internet related materials. We, The Internet Regulatory Survey Team (IRST), are conducting a survey/study pertaining to these issues and are interested in your opinions. We are sending along a small 16 question survey that should take no more than five minutes of your time to complete. We ask for your participation in this study and provide us with your information. Your response will be completely anonymous. If you'd prefer to visit our Web site and fill out the form there the address is: http://eggbeater.olivet.edu/~survey/ Thank You in advance for your time! ----------------------------------------------- Internet Regulatory Survey 1. What service do you use to access the Internet? AOL Compuserve MSN Netcom Local Internet Service Provider (ISP) College or University LAN Other 2. How many times do you use Email during the week? 0-5 6-10 11-15 16-20 20+ 3. How many hours during the week do you spend on the Internet? 0-5 6-10 11-15 16-20 20+ 4. How long have you been using the Internet? 1-6 months 7-12 months 1-2 years 2-3 years 3+ years 5. How would you rate yourself as an Internet user? Beginner Intermediate Advanced Expert For questions 6-11 please indicate the level of agreement or disagreement for each. SA=Strongly Agree A=Agree N=Neutral D=Disagree SD=Strongly Disagree 6. The government should be allowed to regulate the Internet in general. SA A N D SD 7. How do you feel about laws governing material displayed on web sites? SA A N D SD 8. How do you feel about Internet service providers censoring email? SA A N D SD 9. A web site should be able to contain any information it wants no matter how vulgar, obscene, or derogatory it may be. SA A N D SD 10. Web sites should display a warning if they contain any material that may be questionable or offensive to others. SA A N D SD 11. All web sites should register their site with an Internet Censor Rating (ICR) company and display a censor rating, much like that used in television. SA A N D SD 12. Gender: Male / Female 13. Age: 14. Country: __________________ 15. Religious affiliation: Catholic Protestant Jewish Islamic Hindu Buddhist Other None 16. Political party: (U.S. Residents) Democrat Republican Independent Libertarian Other None (Non-US Residents) List political party name: _______________ ------------------------------------------------- We thank you for participation and encourage you to send us your comments and suggestions. Visit our Web site for more info Internet Regulatory Survey Team (IRST) WEB: http://eggbeater.olivet.edu/~survey/ EMAIL: survey@eggbeater.olivet.edu From owner-rapd@net.bio.net Tue Mar 25 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!news-peer.gsl.net!news.gsl.net!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!cpk-news-feed1.bbnplanet.com!ox.mc.edu!usenet From: "Robert G. Hamilton" Newsgroups: bionet.molbio.rapd Subject: Excising and sequencing RAPDs products Date: Wed, 26 Mar 1997 13:15:06 -0500 Organization: Mississippi College Lines: 7 Message-ID: <333967AA.4FA3@csc.mc.edu> Reply-To: rhamilto@csc.mc.edu NNTP-Posting-Host: rhamilto.mc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win16; I) I am interested in analyzing natural populations for poymorphisms. I plan to complete a preliminary RAPDs survey, and they isolate, clone and sequence selected bands, then use sequence data to generate PCR primers, then use PCR primers to amplify and sequence PCR products. Has anyone done this or any aspect of this (we have RAPDs working very well) who might have some advice? From owner-rapd@net.bio.net Tue Mar 25 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!zephyr.texoma.net!uunet!in3.uu.net!192.220.251.22!netnews.nwnet.net!server.umt.edu!not-for-mail From: Donna Newsgroups: bionet.molbio.rapd Subject: FSTAT program Date: Tue, 25 Mar 1997 19:36:17 -0700 Organization: Montana Department of Natural Resources and Conservation Lines: 12 Message-ID: <33388BA1.5848@ism.net> Reply-To: dleeper@ism.net NNTP-Posting-Host: vipond.dbs.umt.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (WinNT; I) Greetings, Does anyone know if the FSTAT program is still available? I tried loging on as instructed in the publication (J Hered 86:485-486) and I couldn't get in. If it is no longer available here, is there somewhere else where it can be down loaded? Thanks Donna Leeper University of Montana dleeper@ism.net From owner-rapd@net.bio.net Sun Mar 30 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!newsfeeds.sol.net!hammer.uoregon.edu!leto.ou.edu!news.ou.edu!news.nodak.edu!badlands!dahleen From: dahleen@badlands.NoDak.edu (Lynn S Dahleen) Newsgroups: bionet.molbio.rapd Subject: Re: Excising and sequencing RAPDs products Date: 31 Mar 1997 18:52:49 GMT Organization: North Dakota Higher Education Network Lines: 16 Message-ID: <5hp161$gbr$1@daily-planet.nodak.edu> References: <333967AA.4FA3@csc.mc.edu> NNTP-Posting-Host: badlands.nodak.edu Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit X-Newsreader: TIN [version 1.2 PL2] converting RAPD bands to specific PCRs is routine, and is being done by many labs. We had a paper describing the use of this technique in Crop Science (1995, 35:1445-1450), and there are lots of other publications out there. Feel free to e-mail me with any specific questions. Lynn Dahleen USDA-ARS, Fargo, ND Robert G. Hamilton (rhamilto@csc.mc.edu) wrote: : I am interested in analyzing natural populations for poymorphisms. I : plan to complete a preliminary RAPDs survey, and they isolate, clone and : sequence selected bands, then use sequence data to generate PCR primers, : then use PCR primers to amplify and sequence PCR products. : Has anyone done this or any aspect of this (we have RAPDs working very : well) who might have some advice? From owner-rapd@net.bio.net Mon Mar 31 23:00:00 1997 Path: biosci!SKYWAY.USASK.CA!ganeshan From: ganeshan@SKYWAY.USASK.CA (Pooba Ganeshan) Newsgroups: bionet.molbio.rapd Subject: Re: Excising and sequencing RAPDs products Date: 1 Apr 1997 06:24:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 54 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <5hp161$gbr$1@daily-planet.nodak.edu> NNTP-Posting-Host: net.bio.net On Mon, 31 Mar 1997, Lynn S Dahleen wrote: > converting RAPD bands to specific PCRs is routine, and is being done by > many labs. We had a paper describing the use of this technique in Crop > Science (1995, 35:1445-1450), and there are lots of other publications > out there. Feel free to e-mail me with any specific questions. > Lynn Dahleen > USDA-ARS, Fargo, ND > > > Robert G. Hamilton (rhamilto@csc.mc.edu) wrote: > : I am interested in analyzing natural populations for poymorphisms. I > : plan to complete a preliminary RAPDs survey, and they isolate, clone and > : sequence selected bands, then use sequence data to generate PCR primers, > : then use PCR primers to amplify and sequence PCR products. > > : Has anyone done this or any aspect of this (we have RAPDs working very > : well) who might have some advice? > > I have done this kind of work with some pathogens of barley. Sequencing the terminal ends of the unique RAPD bands and designing PCR primers is pretty straight forward. However, don't expect that the primers you design would be specific to the sp. you are interested in the first time around. You might have to try several primers before getting what you want. The other thing you would want to pay attention to is primer-dimer formation. Basically follow the rule of thumb for designing PCR primers. The way I approached my primer design was to excised the band of interest from the gel, elute it out of the gel slice, clone it in TA cloning vector, transform E. coli and do a miniprep and do the sequencing. But I have one question. Why do you want to sequence the PCR product after making primers based on the terminal sequence of the RAPD band. Theoretically, this PCR amplified product should have the same sequence as your RAPD fragment sequence! Good luck ******************************************************************* *Pooba S. Ganeshan Phone: (306) 966 8182 (Off.)* *Crop Molecular Genetics Lab. (306) 966 8476 (Lab.)* *Dept. of Crop Science Fax : (306) 966 5015 * *University of Saskatchewan * *51, Campus Drive * *Saskatoon, Saskatchewan * *Canada ***************************** * *S7N 5A8 *Ile Maurice-Paradis Terrestre* ******************************************************************* From owner-rapd@net.bio.net Mon Mar 31 23:00:00 1997 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!news.bbnplanet.com!ox.mc.edu!usenet From: "Robert G. Hamilton" Newsgroups: bionet.molbio.rapd Subject: Re: Excising and sequencing RAPDs products Date: Tue, 01 Apr 1997 13:23:10 -0500 Organization: Mississippi College Lines: 12 Message-ID: <3341528E.7B8@csc.mc.edu> References: <5hp161$gbr$1@daily-planet.nodak.edu> Reply-To: rhamilto@csc.mc.edu NNTP-Posting-Host: rhamilto.mc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win16; I) Pooba Ganeshan wrote: > But I have one question. Why do you want to sequence the PCR product > after making primers based on the terminal sequence of the RAPD band. > Theoretically, this PCR amplified product should have the same sequence as > your RAPD fragment sequence! 1. Confirm homologies 2. Sequences should reveal absolutely all polymorphisms among homologous bands. 3. With primers in hand, sequencing is easier than RAPDs (RAPDs is an excellent means of surveying the genome for polymorphic sequences).