From owner-rapd@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!ACD.TUSK.EDU!PRAKASH From: PRAKASH@ACD.TUSK.EDU Newsgroups: bionet.molbio.rapd Subject: Beachy, Keen and Lander elected to NAS! Date: 2 May 1997 07:08:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IIE05WU9DU000NR6@Acd.Tusk.Edu> NNTP-Posting-Host: net.bio.net Dr. Roger Beachy of Scripps, Dr. Noel Keen of UC Riverside and Dr. Eric Lander of MIT have been elected to the U.s. National Academy of Sciences. More details at http://www.nas.edu. Congratulations to them on this great honor! Prakash From owner-rapd@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!wiz.uni-kassel.de!jutzi From: jutzi@wiz.uni-kassel.de (Samuel Jutzi, by way of C. S. Prakash) Newsgroups: bionet.molbio.rapd Subject: Chair in Agrobiodiversity at Kassel University, Germany Date: 3 May 1997 12:06:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 61 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Position Announcement Chair in Agricultural Biodiversity The Faculty of Agriculture, International Rural Development and Environmental Protection at the University of Kassel, Germany, has an opening for a professoral tenure position (C3) in agricultural biodiversity, to be based at its premises in Witzenhausen, 40 kms north of Kassel. The incumbent shall, in the Faculty's curriculum and research profile (Ecological Agriculture), represent the biological principles of agricultural biodiversity in its socio-cultural, political and economic contexts. The focus is on plant genetic resources (PGR). Apart from the specific subjects related to agro-biodiversity, teaching responsibilities include basic courses on the biology of plants. The incumbent is expected to assume the scientific responsibility for the plant collection in the Greenhouse for Tropical and Subtropical Crops at the Faculty. Priority is with the following research areas: - population dynamics as a function of land use systems of varying complexity and intensity, and relationships between genepools of different orders, - diversity at the variety, species and habitat levels, - in-situ and ex-situ conservation and use of PGR. Desirable are working experiences with traditional knowledge and indigenous use systems of PGR, with the implementation of programmes and agreements for PGR conservation and use in the formal and informal sectors, and with on-farm conservation and enhancement of PGR. Several years of scientific and practical experiences with PGR, and substantial teaching and international research experiences are a prerequisite. The successful candidate will be expected to commit her/himself to the collaborate in the further curricula development at the Faculty, to the advancement of interdisciplinary and applied research, and to the involvement in University self-government. Teaching language is predominantly German. Information on the Faculty is available from the Dean's office: Prof. Dr. G. Spatz (Steinstrasse 19, D-37213 Witzenhausen, Germany, Fax: +49 5542 981309, e-mail: ) Applications with detailed CV, publications list and the particulars of three referees, may be directed (deadline May 31st, 1997; position code nbr. 087) to the President, University of Kassel, Moenchebergstrasse 19, D-34109 Kassel, Germany. The University of Kassel wishes to increase the share of females on its staff; women are therefore particularly encouraged to apply. Physically handicapped applicants are given preference at equal qualification. Dr. Samuel C. Jutzi (Prof) Kassel University, Faculty of Agriculture Institute of Crop Science Dept. of Tropical and Subtropical Field Crops Steinstrasse 19 D 37213 Witzenhausen Federal Republic of Germany phone +49 5542 981 228 fax +49 5542 981 230 From owner-rapd@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!news-peer.sprintlink.net!news.sprintlink.net!Sprint!newsjunkie.ans.net!newsfeeds.ans.net!lantana.singnet.com.sg!newsfeed.singnet.com.sg!id4.nus.edu.sg!nuscc.nus.sg!sbsdjl From: sbsdjl@leonis.nus.sg (ding jeak ling) Newsgroups: bionet.molbio.rapd Subject: Protein modelling job vacancies Date: 3 May 1997 02:02:51 GMT Organization: National University of Singapore Lines: 29 Message-ID: <5ke6cb$pf8@nuscc.nus.sg> NNTP-Posting-Host: sbsdjl@leonis.nus.sg X-Newsreader: TIN [version 1.2 PL2] ......................................................................... "Positions available - for a period of 2 years" (1) Research Fellow with PhD. Prerequisites include experience in computer aided molecular modelling of macroproteins, ligand-binding and conformational studies. Creative flair and ability to solve problems, as exemplified by a strong publication track record are helpful. Salary: commensurate with experience, up to a maximum of US$47,000 per annum (inclusive of 13th month payment and variable bonuses). (2) Research Assistant with BSc Hons. in Cell and Molecular Biology. To carry out genetic engineering experiments and research and development in gene expression. Knowledge in computational molecular biology will be an advantage. Salary: commensurate with qualification and experience, up to US25,000 per annum. (Current Exchange rate is US$1 = S$1.41) Interested applicants should write/enquire and send CV to: JL Ding (PhD) Marine Biotechnology Laboratory School of Biological Sciences National University of Singapore Kent Ridge, Singapore 119260 Tel: 65-7722776 Fax: 65-7792486 E.mail:sbsdjl@leonis.nus.sg .............................................................................. From owner-rapd@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!fao.org!ICPPGR From: ICPPGR@fao.org (ICPPGR, by way of C. S. Prakash) Newsgroups: bionet.molbio.rapd Subject: FAO Plant Breeding Newsletter Date: 3 May 1997 12:07:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Colleagues, Sorry for the long mail and any eventual cross-posting. Find below the annoucement of a new FAO Electronic Plant Breeding Newsletter. Please, forward this announcement to anyone who might be interested. Regards. Dr Eric Kueneman (FAO) & Prof Don Wallace (Cornell University) ================================================================ == ANNOUNCEMENT OF THE ELECTRONIC PLANT BREEDING NEWSLETTER == ================================================================ ================================================================ Aware of the importance of plant breeding as a tool for enhancing agricultural production and its sustainability, the Food and Organization Agriculture (FAO) of the United Nations is hosting an electronic newsletter called PLANT BREEDING NEWS (PBN) designed to promote the exchange of information among all interested persons including plant breeders, researchers, policy makers, crop extension experts, NGOs, students, etc. The newsletter is meant to be your informal forum on plant breeding technologies and related issues including announcements, inquiries, technical discussions, un-refereed articles, etc. Other features will be added as per requests from subscribers. Participation is free and is based on voluntary contributions of information from members. By subscribing you will receive one monthly PBN edition. ================================================================ =================== == TO SUBSCRIBE: == =================== send an email to: MAILSERV@MAILSERV.FAO.ORG Leave the subject blank and write in the first line of the email message: SUBSCRIBE PBN-L You will soon receive a welcome message confirming your subscription. If you have any problems with your subscription, please contact: PBN-L@MAILSERV.FAO.ORG ===================== == TO UNSUBSCRIBE: == ===================== send an email to: MAILSERV@MAILSERV.FAO.ORG leaving the subject blank and writing in the body of your email message: UNSUBSCRIBE PBN-L ========================================== == PURPOSE OF PLANT BREEDING NEWS (PBN) == ========================================== PBN is intended to provide a dynamic forum of information exchange for plant breeders and others interested in effective utilisation of plant genetic resources. The PBN is in response to the needs and actions declared important in The Global Plan of Action for Conservation and Utilisation of Plant Genetic Resources ratified by 150 countries at the Fourth International Technical Conference on Plant Genetic Resources (PGR), Leipzig, Germany, June 1996. The PBN will contribute toward improved utilisation of PGR. The PBN will also promote effective and responsible plant breeding technologies to develop improved varieties, well adapted to the needs of farmers with durable resistance to pests and diseases. By generously sharing technologies, information, germplasm and ideas through PBN, the global plant breeding community can expedite responsible sustainable development and help ensure that the world's agricultural needs are met for today and for the future. ===================== == PARTICIPATION: == ===================== Participation in PBN is free, open to anyone, and based on voluntary contributions by subscribers. It is envisaged that PBN will contribute to improved communications among breeders and experts in both developed and developing countries. The quality of PBN content will depend on you and all other subscribers. PBN NEEDS YOUR INVOLVEMENT!!! To benefit from the PBN, please contribute information, ideas, discussion and queries to the Editor (see below). Remember, that the PBN, though very serious, is intended to be informal. Contributors should not be overly concerned about the level of syntax. It is the exchange of ideas and information that are really important in PBN! ========================== == CONTENT & PROMOTERS: == ========================== Each PBN edition will have a number of sections based on crop groups and thematic issues. The sections may change as needed over time based on your suggestions. The initial section headings are shown at the end of this communication. One or more subscribers may take the role of "promoter" to encourage dialogue and information exchange for sections of specific interest to them. Subscribers wishing to assist promotion of discussion for a section are asked to contact Prof. Wallace. Promoter's contributions will be acknowledged. ============== == EDITOR: == ============== The editor will place the information received into the appropriate section for each monthly issue. Professor Don Wallace of the Plant Breeding and Biometry Department of Cornell University, Ithaca, New York is the first Editor of the PBN. To reach the editor, simply send your email message to: PBN-L@MAILSERV.FAO.ORG writing in the subject: "To the Editor". ============== == SPONSOR: == ============== The FAO provides the List Server facilities for publishing the PBN. FAO's Plant Production and Protection Division and of the Plant Breeding and Genetics Section of the Joint Division of FAO and the International Atomic Energy Agency are co-sponsors. The staff of all three will contribute to this newsletter as part of their programme to bring improved plant breeding technologies to developing countries. It is the intention and our hope that the plant breeding community, globally, finds the PBN an effective means of information exchange. FAO is open to exploring wider sponsorship arrangements. ================= == LIST OWNER: == ================= Mr. Eric Kueneman, Senior Officer in the Plant Production and Protection Division of FAO is the List Owner and PBN Coordinator. He will work with Professor Wallace to facilitate implementation of the PBN. Again should you have any difficulties in receiving the PBN, kindly contact Professor Wallace or Mr. Kueneman at: PBN-L@MAILSERV.FAO.ORG ========================================== == SECTIONS OF THE PLANT BREEDING NEWS: == ========================================== Some communications for the PBN will have primarily a crop orientation, and others a disciplinary orientation. Most may involve both. The following matrix of CROPS GROUPS and DISCIPLINARY AREAS will be used to indicate the subject matter area of each submission that can be located in the PBN. To guide subscribers to the communications they wish to read, the editor will classify all short submissions (3 pages and less) and abstracts presented in each PBN edition according to the crop groups/disciplinary areas presented below CROP GROUP 1. Cereals 2. Roots & Tubers 3. Horticulture 4. Industrial 5. Pulses & Oil-Seed Legumes 6. Pasture and Forage 7. New Crops 8. Applies to all Crops DISCIPLINARY AREA A. Editor's Notes B. Announcements C. General Inquiries D. Technology & Breeding Methods E. Biotechnology F. Diseases & Pests G. Abiotic Stresses H. Breeders Rights I. BioSafety and/or Plant Quarantine J. Policy K. Society & Plant Breeding L . Quantitative traits M. Plant Genetic Resources N. Genetic Vulnerability O. Seed P. Bibliographies Q. Popular WEB Sites ==================== == CONTRIBUTIONS: == ==================== SEND ALL CONTRIBUTIONS, QUERIES AND DISCUSSION FOR THE PBN TO: PBN-L@MAILSERV.FAO.ORG Indicate at the beginning of your contribution the disciplinary and/or crop section in which you wish your communication to be placed. Until published in a PBN issue, your contribution will be known to only the Editor and the List Owners. The PBN will be based on email "text only" format regardless of length of submissions. Please do not send attachments in word processing formats such as Microsoft Word or WordPerfect. LONG SUBMISSIONS Authors must send abstracts of any submission longer than 3 pages in length. The full text of long submissions will be archived. =========================== == ARCHIVING FACILITIES: == =========================== PBN has an archive facility where long submissions (greater than 3 pages) and prior issues of the PBN will be stored for free retrieval. A file name will be given and stated at the end of the abstract in PBN. You can retrieve the full text from the archive by sending an email message to MAILSERV@MAILSERV.FAO.ORG and leave the subject blank and write in the body of the message: SEND [PBN]filename.ext For example, if the file name of the full text that you want to retrieve from the archive is: PBNA3.TXT then in the body of your message to MAILSERV@MAILSERV.FAO.ORG will be: SEND [PBN]PBNA3.TXT MAILSERV will send you the full text of the requested file by email. ================================================================ From owner-rapd@net.bio.net Sat May 03 23:00:00 1997 Path: biosci!POPHOST.EUNET.BE!RvP From: RvP@POPHOST.EUNET.BE (Jochen Dendauw) Newsgroups: bionet.molbio.rapd Subject: AFLP protocol Date: 4 May 1997 08:43:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.1.32.19970504174048.00695cb0@pophost.club.innet.be> NNTP-Posting-Host: net.bio.net Dear newsgroupers, I've got a question about the AFLP-protocol. Since september '95 we started the AFLP-protocol. First we used the Gibco BRL -protocol with a radioactive label , since last year we started the fluorescent protocol from Perkin Elmer on the ABI Prism 377 DNA Sequencer. In those protocols there is no biotine selection for the removal of th e Mse I / Mse I fragments. Can someone give me an explanation why this step is not present in the protocols. Thanks, ing. Jochen Dendauw Plant Breeding Station - RvP Biotechnology Lab e-mail : RvP@pophost.eunet.be tel :+32 9 252 19 81 fax :+32 9 252 11 50 From owner-rapd@net.bio.net Sat May 03 23:00:00 1997 Path: biosci!POPHOST.EUNET.BE!RvP From: RvP@POPHOST.EUNET.BE (Jochen Dendauw) Newsgroups: bionet.molbio.rapd Subject: AFLP's on the ABI 377 Date: 4 May 1997 08:32:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 26 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.1.32.19970504172951.00695cb0@pophost.club.innet.be> NNTP-Posting-Host: net.bio.net Dear newsgroupers, Since last year we started with AFLP reactions on the ABI Prism 377 DNA Sequencer from PE. For the moment we've got a lot of samples, so we want the use the benefit of the machine , the four colour dyes (FAM, JOE, ROX & TAMRA) by mulitplexing the samples. I've tried some experiments but their is great background for the JOE-dye. Does anyone have a protocol for multiplexing AFLP rxns on the ABI 377? Does anyone have experience with the AmpliTaq Gold in AFLP rxns ? Thanks a lot ! ing. Jochen Dendauw Plant Breeding Station - RvP Biotechnology Lab e-mail : RvP@pophost.eunet.be tel : +32 9 252 19 81 fax : +32 9 252 11 50 From owner-rapd@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!MAIL.UNIXG.UBC.CA!jcarlson From: jcarlson@MAIL.UNIXG.UBC.CA Newsgroups: bionet.molbio.rapd Subject: Re: AFLP protocol (fwd) Date: 5 May 1997 09:00:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 56 Sender: daemon@net.bio.net Distribution: world Message-ID: <1.5.4.16.19970505165253.1347e5ae@pop.unixg.ubc.ca> NNTP-Posting-Host: net.bio.net Jochen: Q: re. Mse1/Mse1 fragments: A: Only EcoR1 adopter specific primer is fluorescently tagged, so only products arising from EcoR1/Mse1 fragments will be seen on the gel. Q: Multiplexing protocols and Amplitaq Gold: A: General considerations in constructing multiplexing panels would be, matching Tm s and lack of cross complementarity between primer pairs. Amplitaq Gold is supposed to prevent primer oligomer formation. The web site for PE must have their version of answers to all thi.! Vindhya Amarasinghe University of British Columbia you wrote: > Date: 4 May 1997 08:43:18 -0700 > From: Jochen Dendauw To: rapd@net.bio.net > Subject: AFLP protocol > > Dear newsgroupers, > > I've got a question about the AFLP-protocol. > > Since september '95 we started the AFLP-protocol. > First we used the Gibco BRL -protocol with a radioactive label , since last > year we started the fluorescent protocol from Perkin Elmer on the ABI Prism > 377 DNA Sequencer. > In those protocols there is no biotine selection for the removal of th e > Mse I / Mse I fragments. > Can someone give me an explanation why this step is not present in the > protocols. > > Thanks, > > Jochen Dendauw > > Plant Breeding Station - RvP > Biotechnology Lab > e-mail : RvP@pophost.eunet.be > tel :+32 9 252 19 81 > fax :+32 9 252 11 50 > John E. Carlson Chair, Genetics Graduate Program room 228 Computer Services Building Associate Professor, Biotechnology Laboratory & Departments of Forest Sciences and Plant Science room 237 Wesbrook Building University of British Columbia Vancouver, B.C., CANADA V6T 1Z3 phone # (604) 822-4733 fax # (604) 822-6097 From owner-rapd@net.bio.net Wed May 07 23:00:00 1997 Path: biosci!esvax.dnet.dupont.com!rafalski From: rafalski@esvax.dnet.dupont.com (Antoni Rafalski, by way of C. S. Prakash) Newsgroups: bionet.molbio.rapd Subject: Re: Beachy, Keen and Lander elected to NAS! Date: 8 May 1997 08:48:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear C.S.: Other plant-related people elected to the academy: George Lorimer of DuPont (RUbisco and chaperonins) Dieter Soll of Yale (chlorophyll biosynthesis) antoni -- Antoni Rafalski DuPont Agricultural Biotechnology P.O.Box 80420 Experimental Station Bldg. E402 Room 4248 Wilmington, DE 19880-0402 USA Tel. 302-695-4348 Fax 302-695-4296 e-mail rafalski@esvax.dnet.dupont.com From owner-rapd@net.bio.net Wed May 07 23:00:00 1997 Path: biosci!daresbury!uninett.no!nntp.uio.no!newsfeeds.sol.net!europa.clark.net!feed1.news.erols.com!howland.erols.net!newshub2.home.com!newshub1.home.com!news.home.com!news1.best.com!wizard.pn.com!news.gte.com!news-in.tiac.net!posterchild!news@tiac.net From: Property Digest Newsgroups: bionet.molbio.gene-orgbionet.molbio.genome-program,bionet.molbio.hiv,bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molbio.rapd Subject: new pages on NatBio Web Site Date: Thu, 08 May 1997 12:39:20 -0700 Organization: U.S. Real Estate Register Lines: 9 Message-ID: <33722BE8.954@barryinc.com> NNTP-Posting-Host: p7.ts23.metro.ma.tiac.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win16; I) Xref: biosci bionet.molbio.hiv:3587 bionet.molbio.methds-reagnts:57458 bionet.molbio.proteins:10673 bionet.molbio.rapd:1898 National Biotech Register(NatBio), a comprehensive up-to-date reference guide on research and product development activity in the Biotech Industry, is pleased to announce the addition of two new pages on our web site: http://www.barryinc.com/bio ..The first page is a help wanted in the Biotech field, while the second is a bulletin board page. This page will allow people in the Biotech industry to exchange ideas, search for products and or research services, as well as giving you a forum to announce new discoveries. Come check out these new pages and help expand on them. From owner-rapd@net.bio.net Thu May 08 23:00:00 1997 Path: biosci!greb.ulaval.ca!Christian.Mouton From: Christian.Mouton@greb.ulaval.ca (Christian Mouton) Newsgroups: bionet.molbio.rapd Subject: PCR-DNA Probe Assays for Prevotella intermedia and Prevotella nigrescen Date: 9 May 1997 06:54:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705091357.JAA12434@hermes.ulaval.ca> NNTP-Posting-Host: net.bio.net Here is the abstract of a paper to be published in Journal of Clinical Microbiology: PCR-DNA Probe Assays for Identification and Detection of Prevotella intermedia sensu stricto and Prevotella nigrescens Emmanuelle Guillot and christian Mouton The purpose of this study was to construct PCR-DNA probe assays specific for Prevotella intermedia sensu stricto and Prevotella nigrescens based on the ability of random amplified polymorphic DNA (RAPD) fingerprinting to generate species-specific markers. The strategy included four steps: (i) construction of first generation DNA probes from a 850-bp RAPD marker for P. intermedia sensu stricto and a 1,300-bp RAPD marker for P. nigrescens, (ii) cloning and sequencing of each RAPD marker, (iii) design of primer pairs flanking specific internal sequences of 754 bp for P. intermedia sensu stricto and ca. 1,100 bp for P. nigrescens, and (iv) synthesis (by PCR amplification) and digoxigenin labeling of quantities of DNA probes 754 and ca. 1,100 bp in size. The PCR-DNA probe assays combine either PCR amplification of a 754-bp specific sequence in the genomic DNA of strains of P. intermedia sensu stricto and hybridization with the 754-bp digoxigenin-labeled probe or amplification of a ca. 1,100-bp sequence of P. nigrescens and hybridization with the ca. 1,100-bp probe. Specific hybridization was observed with the amplified DNAs from 25 strains of P. intermedia and 24 strains of P. nigrescens, and no reaction with the PCR products from 20 foreign species. The PCR-DNA probe assays described here should allow a highly specific and sensitive detection of P. intermedia sensu stricto and P. nigrescens in mixed infections. Christian Mouton, DCD, DSO Groupe de Recherche en Ecologie Buccale Faculte de medecine dentaire, Universite Laval Quebec (Quebec) G1K 7P4 CANADA tel. (418)656-5872; fax. (418)656-2861 From owner-rapd@net.bio.net Sun May 11 23:00:00 1997 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!uunet!in3.uu.net!206.229.87.25!news-peer.sprintlink.net!news.sprintlink.net!Sprint!news-peer.gsl.net!news-paris.gsl.net!news.gsl.net!rain.fr!infonie.fr!not-for-mail From: "zougari sadeq" Newsgroups: bionet.biology.n2-fixation,bionet.jobs.wanted,bionet.molbio.proteins,bionet.molbio.rapd,bionet.plants,uk.jobs.wanted Subject: Seeking postdoc in biol.Mol; genetics; Plant breeding; Proteins Biochemistry Date: 12 May 1997 14:43:25 GMT Organization: infonie.fr Lines: 141 Message-ID: <01bc5ee3$70d7a8a0$031965c3@zougari> NNTP-Posting-Host: 195.101.25.3 X-Newsreader: Microsoft Internet News 4.70.1155 Xref: biosci bionet.biology.n2-fixation:709 bionet.jobs.wanted:13126 bionet.molbio.proteins:10717 bionet.molbio.rapd:1906 bionet.plants:15445 ZOUGARI BEN ELKHAYAT My Abdessadeq Address for correspondance: Laboratoire de Biotechnologie et Amélioration des Plantes Institut National Polytechnique, Ecole Nationale Supérieure Agronomique de Toulouse 145, Avenue de Muret F-31076 Toulouse Cedex France Tel: 33 5 61 34 12 64 Fax: 33 5 62 13 65 65 e-mail:sadeq04@infonie.fr As you can see in my Curriculum Vitae, i have my expertise and interests in working on projects, such as plant breeding, diallels analysis, statistics, plant-microbe interactions, molecular genetics and protein biochemistry. Please find enclosed my Curriculum Vitae and the names of tree Professors with whom i have worked. With my best regards. references: C. PLANCHON, Professor head of the Department of Biotechnology and Plant Breeding at l'Institut National Polytechnique de Toulouse - Ecole Nationale Supérieure AgronomiqueToulouse E-mail: planchon@ensat.fr Phone / Fax: 33 5. 62.13.65.65 M.T. ESQUERRE-TUGAYE, Professor Centre de Physiologie Végétale ( Role of Lipoxygenase in plant-microorganism interactions) Université Paul Sabatier de Toulouse - UMR5546 CNRS E-mail: esquerre@cict.fr Phone / Fax: Tél. 33 5.61.55.67.61 ROUGE, Professor Université Paul Sabatier de Toulouse Laboratoire de Biologie Cellulaire Email: rouge@cicit.fr Tèl: 33 5 61 55 66 11 ----------------------------------- CURRICULUM VITAE ------------------------------------ 1991-1996: PhD work in Plant Biotechnology at the University of Paul Sabatier Toulouse III and l'Institut National Polytechnique de Toulouse - Ecole Nationale Supérieure Agronomique under the guidance of Professor Claude Planchon; head of the Department of Biotechnology and Plant Breeding . ------------------------------------------------------------------------ 1990 - 1991: End of studies Diploma (DEA) of Plant Biotechnology. Option: Interaction Plant Micro-organism at the University of Paul Sabatier Toulouse France ------------------------------------------------------------------------ 1989 - 1990: Master of Cellular Biology. Option: Microbiology at the University of Paul Sabatier Toulouse, France ------------------------------------------------------------------------ 1988 - 1989: Licence Diploma of Biochemistry at the University of Paul Sabatier Toulouse, France ------------------------------------------------------------------------ 1986 - 1988: General University studies: Diploma of Biology at the University of Cadi Ayyad, Faculté des sciences Marrakech, Morocco ------------------------------------------------------------------------ June 1986: Bachelorship of Natural Sciences, Marrakech, Morocco ------------------------------------------------------------------------ SPECIALIZATIONS : · Genetic improvement of soybean seed quality: Genetic determinism study of the expression of soluble proteins and lipoxygenases seed contents with a complete diallel between five genotypes, in natural conditions and in pots. ­ RAPD (PCR) in soybean genotype ® diallel Analyse using Griffing et Hayman methods · Statistics analysis: PCSM, Systat, Statitcf and Sigma Plot. · Plant physiology: relations (carbon) C-N (nitrogen), N-mobilization analysis during seed filling peroid · Biochemistry, Enzymology et Immunology: Þ isolation and purification of seed protein by affinity Chromatography on Sephadex G100 Þ determination of nitrogenase activity of soybean nodule using the Acetylène Reduction Assay by gaz Chromatography (DELSI DI 200) Þ determination of soybean seed lipoxygenase activity polarographically using an Oxygraph Hansatech DWI. Þ spectroscopy Analysis: UV direct Spectroscopy ­ UV Difference Spectroscopy Þ Polyacrylamide gel electophoresis using the Phast-system (Pharmacia) Þ Proteins determinations: a- de Kjeldahl Method b- colorimetric Method BCA (Pierce) Þ hemagglutination Technique: determination of hemagglutination activity on standart micro-titration plates Þ Indirect ELISA (Enzyme Linked Immunosorbent Assay) measurement on standart micro-plates using either rabbit polyclonal antibodies against PsA or monoclonal antibody (6,F8) made against the Lathyrus odoratus lectin. REFERENCES 1) ZOUGARI, A., GUY, S., and PLANCHON, C. 1995. Genotypic lipoxygenase variation in soybean seeds and response to nitrogen nutrition. Plant Breeding 114, 313-316. 2) ZOUGARI, A., P., CHÊNE., H. MAZARGUIL., and P. ROUGÊ. 1993. Unfolding of pea lectin in the presence of guanidium chloride and urea. Lectins: Biology, Biochemistry, Clinical Biochemistry. [Ed] Textop. p143-150. 3) ZOUGARI, A., SUC, S., and PLANCHON, C. 1995. Amélioration de la qualité de la graine (improvement of soybean seed quality): – voies génétiques et agronomiques: Paramètres de la qualité de la graine. Groupe pluridisciplinaire de recherche sur le soja, Auzeville, Fév. 1992, 2-6. 4) ZOUGARI, A. 1995. Amélioration de la qualité de la graine Communication orale. Groupe pluridisciplinaire de recherche sur le soja, Auzeville, Fév. 1992. 5) ZOUGARI, A., PLANCHON, C ., and ECOCHARD, R. 1996. Inheritence of protein content and lipoxygenase activity in soybean seed (Glycine max (L.) merr.) in cours of writing. 6) ZOUGARI, A. 1990. Dénaturation de la lectine de pois: approche structurale et biologique. D.E.A U.P.S.Toulouse , 23 p 7) ZOUGARI, A. 1996. Genetic improvement of soybean seed quality (Glycine max (L. ) merr. ): lipoxygenases, and proteins and response to nitrogen nutrition '. PhD thesis INP,119 p. ------------------------------------- SUMMARY OF PhD THESIS ------------------------------------- TITLE: Genetic improvement of soybean seed quality (Glycine max (L. ) merr. ): lipoxygenases, and proteins and response to nitrogen nutrition. ------------------------------------------------------------------------ SUMMARIZES: The use of soybean seed in human foods can be favored by a reduction of the lipoxygenase content. This investigation aim to analyze the variability and the genetic determinism of lipoxygenase and proteins seed contents. The study of lipoxygenase activity, determined polarographically on seeds taken from plants grown under controlled conditions with a mineral or symbiotique nitrogen feeding, shows a large genotypique variability for lipoxygenases contents, enhanced by high dinitrogen fixation. In these same conditions of culture, during the seed filling period, lipoxygenases accumulation appears massive until the physiological maturity stage (R7) and is followed by a partial degradation. The lipoxygenases may play a role in the temporary storage of nitrogen. Finally a genetic study is realized in natural conditions and in pots, with a complete diallel between five genotypes, representative of a great variability for soluble proteins and lipoxygenases seed contents. Hybrids F1 show a strong effect of heterosis for soluble proteins, lipoxygenase 1 and total lipoxygenases contents. For these last, hybrids show a strong homeostatique behavior with weaker contents than those of the parents. Though, values of contents in soluble proteins indicate a positive overdominance as well as a great sensitivity to environnementales variations. Genetic determinism of the expression of soluble proteins and lipoxygenases contents appears complex and linked to significants additive, non-additive and reciprocal effects. Thus, the reduction of lipoxygenases contents and the improvement of proteins contents can be considered on a genetic context implying the nuclear and cytoplasmique genomes together with more efficent dinitrogen fixation, with respect of dinitrogen fixation From owner-rapd@net.bio.net Fri May 16 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 May 1997 02:00:15 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705170900.CAA10692@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-rapd@net.bio.net Thu May 29 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!newsfeed.internetmci.com!howland.erols.net!vixen.cso.uiuc.edu!uwm.edu!news.cse.psu.edu!news3.cac.psu.edu!news.tc.cornell.edu!newsstand.cit.cornell.edu!NewsWatcher!user From: alm13@cornell.edu (Andreas Matern) Newsgroups: bionet.molbio.rapd Subject: Re: BOOK ON RAPD/AFLP/DAF/AP-PCR AND RELATED TECHNIQUES Date: Fri, 30 May 1997 13:30:59 -0400 Organization: Cornell University Lines: 39 Sender: alm13@cornell.edu (Verified) Distribution: world Message-ID: References: <199705301609.RAA16306@ceres.wye.ac.uk> NNTP-Posting-Host: 128 In article <199705301609.RAA16306@ceres.wye.ac.uk>, S.RANADE@WYE.AC.UK ("SHIRISH A. RANADE") wrote: > HELLO NETTERS! > > I AM ON THE LOOK OUT FOR ANY BOOK THAT HAS BEEN PUBLISHED ON THE > RAPD/AFLP/AP-PCR/DAF AND SIMILAR RELATED TECHNIQUES. ANY ONE KNOW OF > THESE? IF SO I WOULD APPRECIATE THE INFORMATION ABOUT THE PUBLISHER OF > THE BOOK. > Please don't type in all capital letters. Do you only want books or primary research articles? A decent book is: Paterson, Andrew H. (ed). 1996. Biotechnology Intelligence Unit - Genome Mapping in Plants. (ISBN 0-12-546590-4) Published by: R. G. Landes Company 909 Pine Street Georgetown, TX 78626 USA phone (512) 863-7762 fax (512) 863-0081 Hope that's useful. Sincerely, -- Andreas Matern alm13@cornell.edu 266 Emerson Hall Dept of Plant Breeding and Biometry Cornell University Ithaca, NY 14853 From owner-rapd@net.bio.net Thu May 29 23:00:00 1997 Path: biosci!WYE.AC.UK!S.RANADE From: S.RANADE@WYE.AC.UK ("SHIRISH A. RANADE") Newsgroups: bionet.molbio.rapd Subject: BOOK ON RAPD/AFLP/DAF/AP-PCR AND RELATED TECHNIQUES Date: 30 May 1997 09:02:20 -0700 Organization: Wye College, University of London Lines: 38 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705301609.RAA16306@ceres.wye.ac.uk> NNTP-Posting-Host: net.bio.net HELLO NETTERS! I AM ON THE LOOK OUT FOR ANY BOOK THAT HAS BEEN PUBLISHED ON THE RAPD/AFLP/AP-PCR/DAF AND SIMILAR RELATED TECHNIQUES. ANY ONE KNOW OF THESE? IF SO I WOULD APPRECIATE THE INFORMATION ABOUT THE PUBLISHER OF THE BOOK. FURTHER, IF ANY OF YOU HAVE WRITTEN OR ARE WRITING SUCH A BOOK, I WOULD BE HAPPY TO LEARN A BIT MORE ABOUT THIS. THE REASON I AM REQUESTING THIS INFORMATION FOR IS A REVIEW AND/OR A BOOKLET ABOUT THESE TECHNIQUES THAT I AM CONSIDERING FOR PUBLICATION IN INDIA. YOU CAN MAIL TO ME AT THE WYE COLLEGE E-MAIL ADDRESS UNTIL JUNE 30, 1997. THEREAFTER, PLEASE SEND YOUR E-MAILS TO SAR@NBRI.SIRNETD.ERNET.IN AND THE REGULAR MAILS TO DR. SHIRISH A. RANADE CENTRE FOR PLANT MOLECULAR BIOLOGY NATIONAL BOTANICAL RESEARCH INSTITUTE RANA PRATAP MARG LUCKNOW 226 001. (U.P.) INDIA. FAX. NOS. : (91) 522 282881, 282849 THANKS IN ADVANCE FOR ANY INPUT ON THIS TOPIC. S.A.RANADE From owner-rapd@net.bio.net Fri May 30 23:00:00 1997 Path: biosci!LAMAR.COLOSTATE.EDU!antolin From: antolin@LAMAR.COLOSTATE.EDU (Michael Antolin) Newsgroups: bionet.molbio.rapd Subject: Postdoctoral Position Date: 31 May 1997 11:59:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 88 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705311900.NAA75626@lamar.ColoState.EDU> NNTP-Posting-Host: net.bio.net POST DOCTORAL POSITION POPULATION GENETICS OF PARASITOID WASPS A post-doctoral position for up to two years is available in the Department of Biology, Colorado State University. The research involves estimating gene flow of two parasitoid wasps (Braconidae) that act as biological control agents of insect pests in agricultural ecosystems. The first is _Bracon hebetor_, a wasp that attacks the larvae of several pyralid moths in fruit and grain, both in storage and in the field. The objective of this study is to examine how gene flow and population structure will influence sex ratio, ovicide (killing of conspecifics' eggs), and the genetics of sex determination of _B. hebetor_. The second system revolves around _Diaeretiella rapae_, which attacks a number of aphid species on cereals and row crops. The objective of this project is to determine how population subdivision has influenced local adaptation by the wasp to different aphid hosts, and how to manipulate wasp populations among a number of plant/aphid hosts to improve biological control of pests like the Russian wheat aphid. The ideal candidate would have background with the evolutionary biology and population genetics of animals, although not necessarily insects, including the use of molecular genetic markers. On the other hand, individuals with much experience researching insects but with limited background in popualtion genetics may also be considered if they are willing to learn how to conduct and analyze population genetic studies based upon both targeted PCR and RAPD-PCR. Please send a CV, an abstract of the dissertation, and names, addresses, and e-mails of three individuals who would be willing to provide letters of recommendation to: Michael F. Antolin Department of Biology Colorado State University Fort Collins, CO 80523 Ph: (970)-491-1911 FAX: (970)-491-0649 E-mail: ANTOLIN@LAMAR.COLOSTATE.EDU Recent publications describing parts of these systems are listed below: Vaughn, T.T. and M.F. Antolin. 1997. Population genetics of an opportunistic parasitoid in an agricultural landscape. Heredity, in press (July). Ode, P.J., M.F. Antolin, and M.R. Strand. 1997. Constrained ovipostion and female-biased sex allocation in a parasitic wasp. Oecologia 109: 547-555. Antolin, M.F., C.F. Bosio, J. Cotton, W. Sweeney, M.R. Strand, and W.C. Black IV. 1996. Intensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with SSCP analysis of RAPD markers. Genetics 143: 1727-1738. Ode, P.J., M.F. Antolin, and M.R. Strand. 1996. Sex allocation and sexual asymmetries in intra-brood competition in the parasitic wasp Bracon hebetor. J. Anim. Ecol. 65: 690-700. Vaughn, T.T., M.F. Antolin, and L.B. Bjostad. 1996. Physiological and behavioral effects of semiochemicals on the aphid parasitoid Diaertiella rapae. Entomologia experimentalis et applicata, 78: 187-196. Antolin, M.F., P.J. Ode, and M.R. Strand. 1995. Variable sex ratios and ovicide in an outbreeding parasitic wasp. Animal Behaviour, 49: 589-600. Antolin, M.F. and M.R. Strand. 1992. Mating system of Bracon hebetor. Ecological Entomology, 17: 1-7. ************************************************************************ Michael F. Antolin Assistant Professor Department of Biology Colorado State University Ft. Collins, CO 80523 U.S.A. Phone: 970-491-1911 FAX: 970-491-0649 E-mail: ANTOLIN@LAMAR.COLOSTATE.EDU From owner-rapd@net.bio.net Sat May 31 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news-out.communique.net!communique!hunter.premier.net!feed1.news.erols.com!newsfeed.internetmci.com!in3.uu.net!203.108.7.42!uunet1.feed.news.ozemail.net!OzEmail-In!news From: Gary Lum Newsgroups: bionet.molbio.rapd Subject: Re: BOOK ON RAPD/AFLP/DAF/AP-PCR AND RELATED TECHNIQUES Date: Sun, 01 Jun 1997 10:00:34 +0930 Organization: http://www.ozemail.com.au/~glum/ Lines: 29 Message-ID: <3390C2AA.3B4A@ozemail.com.au> References: <199705301609.RAA16306@ceres.wye.ac.uk> Reply-To: glum@ozemail.com.au NNTP-Posting-Host: 203.7.177.68 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01Gold (Win95; I) To: "SHIRISH A. RANADE" SHIRISH A. RANADE wrote: > I AM ON THE LOOK OUT FOR ANY BOOK THAT HAS BEEN PUBLISHED ON THE > RAPD/AFLP/AP-PCR/DAF AND SIMILAR RELATED TECHNIQUES. ANY ONE KNOW OF > THESE? IF SO I WOULD APPRECIATE THE INFORMATION ABOUT THE PUBLISHER OF > THE BOOK. Please stop screaming, you must know capitals are very bad netiquette. The best books are put out by the American Society of Microbiology, their press page lists their books, for example, Diagnostic Molecular Microbiology by Persing et al. ASM also have newer issues out with recipes etc. The url for ASM Press is http://www.asmusa.org/press/pre1.htm Regards Gary -- ******************************************************** Dr Gary Lum Director of Microbiology and A/Director of Pathology Royal Darwin Hospital Microbiologists do it with culture and sensitivity Meet me at http://www.ozemail.com.au/~glum/index.html E-mail me at mailto:glum@ozemail.com.au,gary.lum@nt.gov.au