From owner-rapd@net.bio.net Sun Jun 01 23:00:00 1997 Path: biosci!esvax.dnet.dupont.com!rafalski From: rafalski@esvax.dnet.dupont.com (Antoni Rafalski) Newsgroups: bionet.molbio.rapd Subject: Re: BOOK ON RAPD/AFLP/DAF/AP-PCR AND RELATED TECHNIQUES Date: 2 Jun 1997 04:22:11 -0700 Organization: DuPont Co. Agricultural Biotechnology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <3392689C.6E11@esvax.dnet.dupont.com> References: Reply-To: rafalski@esvax.dnet.dupont.com NNTP-Posting-Host: net.bio.net Book that includes the info you need: Nonmammalian Genome Analysis A Practical Guide Bruce Birren and Eric Lai, Eds. Academic Press 1996 Has an article on "Generating and Using DNA Markers in Plants" - includes RAPDs, SSR, AFLP and related techniques. (Rafalski, Vogel, Morgante, Powell, Andre and Tingey) Antoni Rafalski -- Antoni Rafalski DuPont Agricultural Biotechnology P.O.Box 80420 Experimental Station Bldg. E402 Room 4248 Wilmington, DE 19880-0402 USA Tel. 302-695-4348 Fax 302-695-4296 e-mail rafalski@esvax.dnet.dupont.com From owner-rapd@net.bio.net Mon Jun 02 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!zephyr.texoma.net!hunter.premier.net!su-news-hub1.bbnplanet.com!cam-news-feed1.bbnplanet.com!news.bbnplanet.com!williams.edu!news From: Matt Garland Newsgroups: bionet.molbio.evolution,bionet.molbio.rapd,bionet.population-bio Subject: RAPD analysis of bird blood Date: Tue, 03 Jun 1997 11:46:19 -0500 Organization: Williams College, Williamstown, MA Lines: 10 Message-ID: <33944A58.3C0E@williams.edu> NNTP-Posting-Host: bio_hwilliams_lab3.williams.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) Xref: biosci bionet.molbio.evolution:6208 bionet.molbio.rapd:1933 bionet.population-bio:2489 Hi, Could anyone point me in the direction of some good protocols for RAPD PCR analysis of blood samples (particularly bird blood)? Please respond via e-mail as well as posting to the newsgroup. Thanks much, Matt Garland Williams College From owner-rapd@net.bio.net Tue Jun 03 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!uwm.edu!newsfeeds.sol.net!europa.clark.net!newsfeed.internetmci.com!status.gen.nz!news.express.co.nz!manawatu.planet.co.nz!manawatu.gen.nz!news From: Paul Hirst Newsgroups: bionet.molbio.rapd Subject: Amplification Products in Negative Controls Date: Thu, 05 Jun 1997 10:32:36 +1300 Organization: PlaNet (NZ) Manawatu, +64 6 357-9245, +64 6 367-9944 Lines: 9 Message-ID: <3395DEF4.7034@manawatu.gen.nz> NNTP-Posting-Host: ppp66-205.manawatu.gen.nz Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Win95; I) Hi, I've done some RAPD primer screening and end up with bands (of different (random) sizes compared to when template DNA is added) in my negative (water instead of template DNA) control. I've heard primer extension and primer dimer formation as a reason for this, but fail to understand how this explains for the large product sizes which I am seeing (up to 1.5 kb). Any explanations or references would be most appreciated. thanks, Paul Hirst. From owner-rapd@net.bio.net Wed Jun 04 23:00:00 1997 Path: biosci!SDCC14.UCSD.EDU!cniles From: cniles@SDCC14.UCSD.EDU (Corey Niles) Newsgroups: bionet.molbio.rapd Subject: unsuscribe Date: 5 Jun 1997 11:25:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <199706021227.IAA11411@csp198.erie.ge.com> Reply-To: cniles@ucsd.edu NNTP-Posting-Host: net.bio.net please unsubscribe me From owner-rapd@net.bio.net Wed Jun 04 23:00:00 1997 From: mmangan Newsgroups: bionet.molbio.rapd Subject: (no subject) Date: 5 Jun 1997 22:44:07 GMT Organization: Ireland On-Line Lines: 10 Message-ID: <5n7ffn$jaq$4@nuacht.iol.ie> NNTP-Posting-Host: dialup-302.dublin.iol.ie Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22KIT (Windows; I; 16bit) Path: biosci!rutgers.rutgers.edu!gatech!news1.mid-ga.com!news.hom.net!nntp.mid-ga.com!news.oru.edu!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!newsfeed.internetmci.com!iol!not-for-mail Hello, If you know of any molecualr biolgy (sorry no plants or fish) / medical microbiology work/post-docs in South western Sweden, can you let me know the details ? Thanks, mmangan@iol.ie From owner-rapd@net.bio.net Wed Jun 04 23:00:00 1997 Path: biosci!UOW.EDU.AU!Al_Griskaitis From: Al_Griskaitis@UOW.EDU.AU ("Al Griskaitis") Newsgroups: bionet.molbio.rapd Subject: unsuscribe Date: 5 Jun 1997 16:37:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Please unsuscribe me. Al Griskaitis From owner-rapd@net.bio.net Wed Jun 04 23:00:00 1997 Path: biosci!INTERCHG.UBC.CA!jcarlson From: jcarlson@INTERCHG.UBC.CA (John Carlson) Newsgroups: bionet.molbio.rapd Subject: unsuscribe Date: 5 Jun 1997 12:22:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <1.5.4.16.19970605202239.1e075b88@pop.interchange.ubc.ca> NNTP-Posting-Host: net.bio.net please unsubscribe me John E. Carlson Chair, Genetics Graduate Program room 228 Computer Services Building Associate Professor, Biotechnology Laboratory & Departments of Forest Sciences and Plant Science room 237 Wesbrook Building University of British Columbia Vancouver, B.C., CANADA V6T 1Z3 phone # (604) 822-4733 fax # (604) 822-6097 From owner-rapd@net.bio.net Sun Jun 08 23:00:00 1997 Path: biosci!news.Stanford.EDU!bloom-beacon.mit.edu!newsfeed.internetmci.com!newsfeed.internetmci.com!feeder.chicago.cic.net!chi-news.cic.net!news.indiana.edu!pumpkin.bio.indiana.edu!user From: dewolf@bio.indiana.edu (Diana Wolf) Newsgroups: bionet.molbio.rapd Subject: Re: Amplification Products in Negative Controls Date: Sat, 07 Jun 1997 00:55:19 -0600 Organization: Indiana University Lines: 24 Message-ID: References: <3395DEF4.7034@manawatu.gen.nz> NNTP-Posting-Host: pumpkin.bio.indiana.edu I don't know why either, but we get the same thing in our lab. People in the lab have guessed that it might be from amplification of bacterial DNA that is contaminating the TAQ (the TAQ is isolated from bacteria) or from amplification of DNA that was floating around in the air and floated into the reaction. Diana In article <3395DEF4.7034@manawatu.gen.nz>, Paul Hirst wrote: > Hi, > > I've done some RAPD primer screening and end up with bands (of different > (random) sizes compared to when template DNA is added) in my negative > (water instead of template DNA) control. I've heard primer extension > and primer dimer formation as a reason for this, but fail to understand > how this explains for the large product sizes which I am seeing (up to > 1.5 kb). Any explanations or references would be most appreciated. > thanks, Paul Hirst. -- Diana Wolf Biology Department Indiana University From owner-rapd@net.bio.net Sun Jun 08 23:00:00 1997 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.gsl.net!uwm.edu!rutgers.rutgers.edu!ultra150.ncren.net!nntp-xfer.ncsu.edu!newton.uncg.edu!Mo.uncg.edu!nstewart From: nstewart@goodall.uncg.edu Newsgroups: bionet.molbio.rapd Subject: rapd bood Date: Mon, 2 Jun 1997 09:17:51 Organization: University of North Carolina at Greensboro Lines: 4 Message-ID: NNTP-Posting-Host: n-stewa2.uncg.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Fingerprinting Methods Based on Arbitrarily Primed PCR. 1997. MR Micheli and R Bova (Eds) Springer Lab Manual. Cheers, Neal From owner-rapd@net.bio.net Fri Jun 13 23:00:00 1997 Path: biosci!agate!newsfeed.kornet.nm.kr!news.maxwell.syr.edu!newsfeed.internetmci.com!news2.fibr.net!news1.fibr.net!news.fibr.net!news From: Newsgroups: bionet.molbio.rapd Subject: http://www.planetfun.com/videosex.htm Date: 14 Jun 1997 05:49:53 GMT Lines: 3 Message-ID: <5ntbe1$eb3@nimitz.fibr.net> NNTP-Posting-Host: port1-6.lconn.com http://www.planetfun.com/videosex.htm Adults Only From owner-rapd@net.bio.net Mon Jun 16 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Jun 1997 02:00:06 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199706170900.CAA24291@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-rapd@net.bio.net Wed Jun 18 23:00:00 1997 Message-ID: <33A16EC5.4616@scicentral.com> Date: Fri, 13 Jun 1997 12:01:09 -0400 From: SciCentral Reply-To: scicentral@scicentral.com Organization: SciLink X-Mailer: Mozilla 3.0Gold (Win95; I) MIME-Version: 1.0 Newsgroups: bionet.molbio.oncogenes,bionet.molbio.plant,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r,bionet.molbio.proteins.fluorescent,bionet.molbio.rapd,bionet.molbio.recombination,bionet.molbio.yeast Subject: BEST MOLECULAR BIOLOGY DIRECTORIES Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit NNTP-Posting-Host: 166.72.73.161 Lines: 35 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsm.ibm.net!ibm.net!news1.ibm.net!166.72.73.161 Xref: biosci bionet.molbio.proteins:11016 bionet.molbio.proteins.7tms_r:1290 bionet.molbio.proteins.fluorescent:1305 bionet.molbio.rapd:1952 bionet.molbio.recombination:491 bionet.molbio.yeast:7250 Dear Colleagues, We invite you to attend an opening in your honor at: http://www.scicentral.com This unique Web site has been created for scientists and engineers by scientists who know your needs. Just three clicks will get you everywhere. http://www.scicentral.com includes only the most valuable directories on the Web. They currently constitute a gateway to over 50,000 scientific sites pertaining to over 120 specialties in science and engineering ranging from health, biological, earth, physical, and engineering sciences, to government and institutional listings, and including the Commerce Business Daily and Medline--all free. We envision http://www.scicentral.com as the science and engineering hub of the World Wide Web, a place for professionals to gather and discuss the complex issues facing us all as we approach the 21st century. This is where you can make your opinions known. We plan editorials, chat rooms, news. We will continue to be responsive to your suggestions. Please visit http://www.scicentral.com often and bring your colleagues. Ellen S. Uffen, Ph.D. President, SciLink, Inc. Guy Orgambide, Ph.D. Chief Executive Officer SciLink, Inc. Robert L. Uffen, Ph.D Professor Emeritus Michigan State University From owner-rapd@net.bio.net Thu Jun 19 23:00:00 1997 Path: biosci!byu.edu!JLFarmer From: JLFarmer@byu.edu (James Farmer) Newsgroups: bionet.molbio.rapd Subject: Would you like to moderate this newsgroup? Date: 20 Jun 1997 13:26:05 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.1.32.19970620142620.006a8814@ucspop.byu.edu> NNTP-Posting-Host: net.bio.net I am officially the discussion leader, and I have been taking care of things such as requests to subscribe or unsubscribe, but I do not have enought time to moderate the group (screen messages before they are posted). In the past, I have resisted requests to moderate the group, but perhaps that is going to be necessary. The commercial and pornographic postings are becoming extremely obnoxious. If any of you feel that it is time to begin screening messages, perhaps one or more of you would like to volunteer. If so, please let me know who you are by SENDING ME A PERSONAL MESSAGE. Let's not clutter up the news with this kind of stuff. You can get information on the moderators' duties from biosci-help@net.bio.net Thanks for your help and suggestions. James Farmer Dept. of Zoology Brigham Young University Provo, Utah 84602, USA JLFarmer@byu.edu From owner-rapd@net.bio.net Fri Jun 20 23:00:00 1997 Path: biosci!rutgers!gatech!csulb.edu!hammer.uoregon.edu!leto.ou.edu!news.ou.edu!news.ecn.uoknor.edu!munnari.OZ.AU!bunyip.cc.uq.edu.au!not-for-mail From: "Ian Mackay" Newsgroups: bionet.molbio.rapd Subject: Control for RT-PCR Date: 21 Jun 1997 02:33:41 GMT Organization: University of Queensland Lines: 11 Message-ID: <01bc7deb$2dc4f3c0$41386682@svimacka.ppp.uq.edu.au> NNTP-Posting-Host: svimacka.slip.cc.uq.edu.au X-Newsreader: Microsoft Internet News 4.70.1155 Can anyone suggest a good control to monitor RNA extraction from serum and CSF? I know spiking a sample with RNA works - but is there any RNA that can survive a serum/CSF environment that could be used as a marker of extraction (similarly to the use of b-actin detection for extraction from cellular samples)? Thanks Ian (Please mail me at: I.Mackay@mailbox.uq.edu.au) From owner-rapd@net.bio.net Wed Jun 25 23:00:00 1997 Path: biosci!LEONIS.NUS.SG!sbsrjh From: sbsrjh@LEONIS.NUS.SG (Ric) Newsgroups: bionet.molbio.rapd Subject: What about Lynch's corrected SI estimates? Date: 25 Jun 1997 20:41:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <33B1E49D.26DE@leonis.nus.sg> Reply-To: sbsrjh@leonis.nus.sg NNTP-Posting-Host: net.bio.net I was hoping to muster some interest in debating pros and cons of different methods of statistical analysis of population RAPD data. So... 1) Consider polymorphic bands and treat data similar to protein data (seemingly increasingly popular) or 2) Consider all bands (mono and poly) and use SI values (with Lynch's corrected variances, 1990). Personally I prefer SI values whereby we go on to correct inter-population for intra-population variation and then we test to see if the inter-pop differences are significantly different to zero (example pop A is significantly different to pop B P<0.001). Can anyone build up a list of pros and cons? James Dr James Nelson, School of Biological Sciences National University of Singapore Singapore From owner-rapd@net.bio.net Thu Jun 26 23:00:00 1997 Path: biosci!ZJAU.EDU.CN!lidb From: lidb@ZJAU.EDU.CN (Li Debao) Newsgroups: bionet.molbio.rapd Subject: call paper of the 3rd international symposium on plant pathology and biotechnology Date: 26 Jun 1997 21:14:34 -0700 Organization: Zhou Xueping Lines: 118 Sender: daemon@net.bio.net Distribution: world Message-ID: <33B2122E.26D9@zjau.edu.cn> Reply-To: lidb@public.hz.zj.cn NNTP-Posting-Host: net.bio.net Dear Sir/Madam, Encloseed this E-mail id the 2nd announcement of the 3rd international symposium on plant pathology and biotechnology which will be held in Hangzhou, from Nov.2 - Nov.7 China. We hope you could attend the symposium, your contribution are appreciated to us and all of our precipitant very much. Many Thanks to your kindly reply Xu Jianping 3RD HANGZHOU INTERNATIONAL SYMPOSIUM ON PLANT PATHOLOGY AND BIOTECHNOLOGY HANGZHOU, P. R .CHINA (Second Announcement) November 2 - November 7, 1997 The 3rd Hangzhou International Symposium on Plant Pathology and Biotechnology will be held at the Biotechnology Institute, Zhejiang Agricultural University (BIZAU) from November 2 - November 7, 1997, sponsored by the Chinese Plant Pathology Society (CPPS) and National Natural Science Foundation of China (NNSFC). The Scientific Committee of Symposium Honor Chairman: Professor Liu, Yi., chairman of the Chinese Plant Pathology Society (CPPS) Chairman: Professor Li, Debao., vice chairman of the Chinese Plant Pathology Society and the director of BIZAU. Vice Chairman: Professor Zheng, Zhong., Dean of Plant Protection, Zhejiang Agricultural University Professor Xie, Lianghui., The member of Chinese Science Academic Sinica, Fujian Agricultural University Members: He, Liyuan., Zhang, Binxing., Wen, Fujiang., Jia, Shirong., Xu, Ping., Chen, Jianping., Zhou, Xueping., N. Talbot., Wang, Jisheng., Kang, Zhengsheng., Wang, Dafang., Chen, Yongxuan., Shun, Shuyuan., Tian, Fenxiu., Zhang, Zhongyi., Liu, Shongling., Chen Lihua. Secretariat ( Liaison Office) Zhou, Xueping, Biotechnology Institute, ZAU Xu, Jianping, Biotechnology Institute, ZAU Wu, Yuliang, Biotechnolgy Institute, ZAU Fax: +86-571-696 1525 Telephone: +86-571- 697 1182, 697 1184 E-mail: lidb@public.hz.zj.cn SCIENTIFIC PROGRAM Plenary Lectures: N. Talbot (Exeter University, UK): Molecular Genetics of Infection Related Development in Magnaporthe grisea Dong, J., (Novatis Seeds Company, USA): The Research Progress of Novel Genes of Disease Control of Vegetables, Fruits and Corn (Temporary) La, Yong-Joon., (College of Agriculture and Life Sciences, Seoul National University, Korea): Use of high-density latex agglutination test for detection of plant viruses Tien Po (Institute of Microbiology, CAS): Plant Virus Satellite RNA and its roles in Plant Virus Disease Control (Temporary) Tang Jiliang (Guanxi Agricultural University): The Pathogenesis of Bacterial Plant Pathogens Fang Rongxiang (Institute of Microbiology, CAS): Molecular Aspects of Rice Virus The plenary lectures may be changed in case. The scientific program may include some work groups as following: W01: Mechanisms of Disease Resistance, Plant-Pathogen Interaction and Molecular Aspects of Pathogenesis W02: Structure and Expression of Virus Genome, and Novel Approaches to Diagnosis and Detection of Pathogens and Diseases W03: Biotechnology of Plant Diseases, Transgenic Plants REGISTRATION: The symposium will be held at International Exchange Center of Zhejiang Agricultural University in the campus, individuals who can not register for symposium before October 20, 1997, can registrant at the opening session. The campus of ZAU is near to the famous sightseeing scenic spot Westlake, it is less than 20 minuets bus way to it. The 100 USD registration fee for foreigner and 500 RMB for Chinese is required. The accommodation includes food is approximately 40 $ per day. Graduate students pay half of the cost. REQUIREMENT OF PAPER Space is available for posters. Researchers with new results and data in the areas relevant to plant pathology and biotechnology are encouraged to submit abstracts for poster display to ensure the broadest possible coverage and exchange of recent advances. Please notice the deadline for the submission of the abstract is Aug. 15, 1997. One page abstract should be written in English, typed in single spaced using 10-12 characters per inch, side margined of 1 1/4 inches (3 cm) and printed in a size of A4 in duplicates. Please mail them with a copy of abstract as a Microsoft Word for Windows 95 or Windows 3.X or Macintosh file on a floppy diskette. Please be sure to indicate the authors and their mailing addresses and e-mail addresses. The poster board will be 30 X 40 inches (about 75 cm X 95 cm) for each one. The title should be printed with letters at least 1 inch (2.4 cm). The official invitation will be forwarded on request. SPECIAL SUPPORTS Limited finacial support will be avaliable, all or part of accommodation fee for limited authors might be exempted. From owner-rapd@net.bio.net Thu Jun 26 23:00:00 1997 Path: biosci!OEB.HARVARD.EDU!wamaral From: wamaral@OEB.HARVARD.EDU (Weber Amaral) Newsgroups: bionet.molbio.rapd Subject: (none) Date: 27 Jun 1997 14:08:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe wamaral@oeb.harvard.edu Weber Amaral Harvard University 22 Divinity Ave Cambridge - MA - 02138 From owner-rapd@net.bio.net Thu Jun 26 23:00:00 1997 Path: biosci!MAILBOX.SYR.EDU!ksmyth From: ksmyth@MAILBOX.SYR.EDU (Kerrie-Ann Smyth) Newsgroups: bionet.molbio.rapd Subject: (none) Date: 27 Jun 1997 13:24:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe rapd@net.bio.net Dr. Kerrie-Ann Smyth Biological Research Laboratories Syracuse University Syracuse, NY 13244 Tel: 1-315-443-3695 fax: 1-315-443-2012 From owner-rapd@net.bio.net Fri Jun 27 23:00:00 1997 Path: biosci!CROP.UOGUELPH.CA!GPENA From: GPENA@CROP.UOGUELPH.CA ("Gisela Pena") Newsgroups: bionet.molbio.rapd Subject: MAS Date: 28 Jun 1997 08:40:57 -0700 Organization: Crop Science, The Univ. of Guelph Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi RAPDers: I'm trying to find out if there is a released crop variety (non ongoing programs or theoretical proposals) that has been produced using Mark Assisted Selecion. Does any one can provide me with this information? Thanks in advance.