From owner-rapd@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!IS.DAL.CA!har1 From: har1@IS.DAL.CA ("Harold A. Robertson") Newsgroups: bionet.molbio.rapd Subject: (none) Date: 2 Jul 1997 08:33:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199707021534.MAA66828@is.dal.ca> NNTP-Posting-Host: net.bio.net unsubscribe rapd@net.bio.net From owner-rapd@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!IS.DAL.CA!har1 From: har1@IS.DAL.CA ("Harold A. Robertson") Newsgroups: bionet.molbio.rapd Subject: (none) Date: 2 Jul 1997 08:37:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199707021538.MAA71826@is.dal.ca> NNTP-Posting-Host: net.bio.net unsubscribe har1@is.dal.ca From owner-rapd@net.bio.net Tue Jul 01 23:00:00 1997 Path: biosci!INDYVAX.IUPUI.EDU!PVIJAYAR From: PVIJAYAR@INDYVAX.IUPUI.EDU Newsgroups: bionet.molbio.rapd Subject: (none) Date: 2 Jul 1997 09:22:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IKRCP5QRAA00DHHW@INDYVAX.IUPUI.EDU> NNTP-Posting-Host: net.bio.net unsubscribe pvijayar@indyvax.iupui.edu From owner-rapd@net.bio.net Wed Jul 02 23:00:00 1997 Path: biosci!ACS.UCALGARY.CA!twhidden From: twhidden@ACS.UCALGARY.CA (Troy Whidden) Newsgroups: bionet.molbio.rapd Subject: Re: IMPORTANT INFORMATION Date: 2 Jul 1997 21:26:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <5peiff$e5r@halley.pi.net> NNTP-Posting-Host: net.bio.net Do not send any more information regarding your perverted web page. ------------------------------------------------ On 2 Jul 1997, PASSWORDS we give.. wrote: > > > POSTMASTERS: > > We are accused of SPAMMING! We did not, but they closed our account! > ********** > > Now we take REVENGE! > Here's our SPAMMING!!! > > > HOT ADULT SITE (was the Adult Site of the Week) > > > JOIN the MegAdult Club > > UNLIMITED live sex!!!!! > > For only $ 20 a month!! NO PAY per minute! > > COME and Join our XXX- CLUB > > Http://WWW.FANTASY-DREAMS.NL > > > From owner-rapd@net.bio.net Sat Jul 05 23:00:00 1997 Path: biosci!rutgers!gatech!howland.erols.net!news.apfel.de!univ-lyon1.fr!jussieu.fr!oleane!pressimage!news1.isdnet.net!usenet From: "Vanhée" Newsgroups: alt.music.gangsta.rap,alt.music.rap-metal,alt.rap.lyrics,alt.rap.sucks,alt.rap-gdead,alt.sports.football.pro.dallas-cowboys.erik.williams.rape.rape.rape,alt.swp.socialist.mark.curtis.guilty.of.child.rape,bionet.molbio.rapd,talk.rape,viwa.music.ta Subject: about all rap and hip hop Date: 6 Jul 1997 00:37:05 GMT Lines: 11 Message-ID: <01bc89a4$a0d22620$9ebc95c2@pavanhee> NNTP-Posting-Host: ppp79.lil.hol.fr X-Newsreader: Microsoft Internet News 4.70.1157 Great site about all rap and hip hop Check this http://www.mygale.org/03/rap2/ Forever -- RAP --> http://www.mygale.org/03/rap2/ <- -HIP HOP Email: cut@iname.com From owner-rapd@net.bio.net Sun Jul 06 23:00:00 1997 Path: biosci!LEONIS.NUS.SG!sbsrjh From: sbsrjh@LEONIS.NUS.SG (Richard Hoddell) Newsgroups: bionet.molbio.rapd Subject: What about Lynch's corrected SI estimates? Date: 7 Jul 1997 00:24:03 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <33C09938.5385@leonis.nus.sg> Reply-To: sbsrjh@leonis.nus.sg NNTP-Posting-Host: net.bio.net I was hoping to muster some interest in debating pros and cons of different methods of statistical analysis of population RAPD data. So... 1) Consider polymorphic bands and treat data similar to protein data (seemingly increasingly popular) or 2) Consider all bands (mono and poly) and use SI values (with Lynch's corrected variances, 1990). Personally I prefer SI values whereby we go on to correct inter-population for intra-population variation and then we test to see if the inter-pop differences are significantly different to zero (example pop A is significantly different to pop B P<0.001). Can anyone build up a list of pros and cons? James Dr James Nelson, School of Biological Sciences National University of Singapore Singapore From owner-rapd@net.bio.net Tue Jul 08 23:00:00 1997 Path: biosci!ACE.ACADIAU.CA!msnyder From: msnyder@ACE.ACADIAU.CA ("Marlene Snyder") Newsgroups: bionet.molbio.rapd Subject: unsubscribe Date: 9 Jul 1997 13:28:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <38CA7D498A@ace.acadiau.ca> NNTP-Posting-Host: net.bio.net unsubscribe rapd@net.bio.net From owner-rapd@net.bio.net Tue Jul 08 23:00:00 1997 From: Damn Yankee Newsgroups: bionet.molbio.rapd Organization: Yankee Inc. Subject: !!!Hello!!! NNTP-Posting-Host: 205.139.183.49 Message-ID: <33c0b448.0@nntp.kalnet.net> Date: 7 Jul 97 09:18:00 GMT Lines: 9 Path: biosci!agate!usenet.INS.CWRU.Edu!HSNX.wco.com!newsfeed.dacom.co.kr!newsfeed.internetmci.com!pull-feed.internetmci.com!nntp.kalnet.net!205.139.183.49 Are you looking for a great place to find interesting things on the WWW??? Than look no more because you have found the right place!!! The place you are looking for is Yankee Inc.!!! A great Web Site full of interesting links to interesting places!!! Everything from Adult to Xzlyaphone it's here!!! There is something for everyone and then some!!! So give it a try what do you have to lose except some sleep??? Yankee Inc. http://www.kalnet.net/yank714 Yankee Inc. Your Alternative Web Solution!!! Yankee Inc. http://www.kalnet.net/yank714 If you would like to be removed from my mailing list - hit reply and type "REMOVE" and I will promptly remove you from my list!!! Thank You!!! From owner-rapd@net.bio.net Tue Jul 08 23:00:00 1997 Path: biosci!BRADLEY.BRADLEY.EDU!rrs From: rrs@BRADLEY.BRADLEY.EDU (Robert Stephens) Newsgroups: bionet.molbio.rapd Subject: unsubscribe Date: 9 Jul 1997 13:56:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe rapd@net.bio.net rrs@bradley.bradley.edu Robert Rhea Stephens Biology Department Bradley University Peoria, IL 61625 Phone: 309.677.3013 FAX: 309.677.3558 From owner-rapd@net.bio.net Wed Jul 09 23:00:00 1997 From: randy97 Subject: http://www.love.com Newsgroups: bionet.molbio.rapd NNTP-Posting-Host: pgh.nauticom.net Message-ID: <33c528d2.1@pgh.nauticom.net> Date: 10 Jul 97 18:24:18 GMT Lines: 9 Path: biosci!bcm.tmc.edu!cs.utexas.edu!chi-news.cic.net!newsfeed.internetmci.com!pgh.nauticom.net!pgh.nauticom.net Looking to find people in your area that enjoy the same things as this newsgroup? Check out http://www.love.com It's free, it's new, and it's awesome. Rand From owner-rapd@net.bio.net Wed Jul 09 23:00:00 1997 From: Damn Yankee Newsgroups: bionet.molbio.rapd Organization: Yankee Inc. Subject: I Am Very Sorry!!! NNTP-Posting-Host: 205.139.183.55 Message-ID: <33c25560.1@nntp.kalnet.net> Date: 8 Jul 97 14:57:36 GMT Lines: 5 Path: biosci!rutgers!gatech!howland.erols.net!newsfeed.internetmci.com!pull-feed.internetmci.com!nntp.kalnet.net!205.139.183.55 I would like to apologise to this newsgroup and everyone who reads this newsgroup!!! I promise never to post or send spam to this or any other newsgroup that does not pertain to my posting!!! Please accept my humble apology and again I will never post spam here again!!! Thank You!!! Andrew Schero yank714@kalnet.net From owner-rapd@net.bio.net Wed Jul 09 23:00:00 1997 Path: biosci!mmm.fi!jussi.tammisola From: jussi.tammisola@mmm.fi ("Tammisola Jussi") Newsgroups: bionet.molbio.rapd Subject: (none) Date: 10 Jul 1997 00:11:46 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <9707101012.aa51@lanmthnt.mmm.fi> NNTP-Posting-Host: net.bio.net unsubscribe rapd@net.bio.net jussi.tammisola@mmm.fi Jussi Tammisola, DrSc(Agr&For) Senior Research Advisor Ministry of Agriculture and Forestry Department of Agriculture Quality and Plant Health Unit (Kaisaniemenk. 4 A, 4th Floor) P.O.Box 232 FIN-00171 Helsinki Fax: +358 9 160 2443, Phone: +358 9 160 88 656 From owner-rapd@net.bio.net Wed Jul 09 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news.maxwell.syr.edu!news.oru.edu!news.uoregon.edu!newsfeed.orst.edu!news.orst.edu!gus.FSL.ORST.EDU!krutovsk From: krutovsk@fsl.orst.edu (Konstantin Krutovskii) Newsgroups: bionet.molbio.rapd Subject: AFLP markers Date: Thu, 10 Jul 1997 15:00:20 Organization: Forestry Sciences Lab Lines: 71 Message-ID: NNTP-Posting-Host: gus.fsl.orst.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Dear colleagues, I study QTL mapping in Douglas-fir using hybrids between coastal and interior races and DNA markers in Steve Strauss' lab. RAPD loci were highly heterozygous in hybrids as expected and nearly half of RAPD loci were segregating. Surprisingly, unlike RAPDs, AFLP markers turned out to be nearly monomorphic! We use DNA isolated from megagametophytes from germinated seeds using the same protocol for both RAPD and AFLP. Genetic basis for both types of variation should be practically the same, that is 10 bp relatively random distributed annealing sites (RAPD) vs. relatively random distributed restriction+1+3 selective nucleotide sites (AFLP). So, one should expect that many among all AFLP markers would segregate, but we can hardly find a few. We have tested all 64 pairwise combinations of selective AFLP fluorecent dye labelled primers provided by PE/ABI kit. One third of them worked well with Douglas-fir megagametophyte DNAs giving dozens if not hundreds of good looking fragments. However, only 2-3% of fragments were segregating! I am puzzled "why?" I have no reasonable explanations. I considered the following possible reasons: 1) Selective or preferential amplification of organelle (likely mitochondrial) DNA. Although it is still highly possible, can it explain that we have only a few segregating markers? Why didn't it also affect RAPDs? 2) AFLP fragments, that we observed, are amplified from fungi, bacterial or other DNA contaminating megagametophyte tissue. (The other than megagametophyte contamination is excluded, because we use maize DNA as a control and we didn't observe any single amplified fragment shared by both megagametophyte and maize DNAs.) Again, although it is still possible that megagametophyte DNA is contaminated by fungi or bacteria, can it explain that our primers amplified mostly contaminated DNA and we have only a few segregating markers? 3) Can peculiarity of Douglas-fir genome explain the problem? To address it we are now doing AFLP segregation analysis using megagametophyte DNAs from pine hybrids. Some of you have a lot of experience in genetic mapping using DNA markers, including RAPDs and possibly AFLP now. I wonder if anyone of you have encountered a similar problem before. I hope that some of you could give advice or any idea with what is going on. I would appreciate any of your suggestions and recommendations. Please, don't hesitate to contact me if you need more information and feel free to forward this message to anybody who may help with this problem. Thank you. Sincerely, Kostya Dr. Konstantin Krutovskii Dept. of Forest Science Oregon St. Univ. FSL 020, Corvallis, OR 97331-7501 Phone (541) 737-6564 or -6562 Fax: (541) 737-1393 E-mail krutovsk@ccmail.orst.edu From owner-rapd@net.bio.net Fri Jul 11 23:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!news-peer.sprintlink.net!news-pull.sprintlink.net!news-in-east.sprintlink.net!news.sprintlink.net!Sprint!202.243.48.20!news.gol.com!not-for-mail From: "Paul Sully" Newsgroups: bionet.molbio.rapd Subject: Japanese Professor Date: 11 Jul 1997 23:47:56 GMT Organization: Paul Sully International Academy Lines: 11 Message-ID: <01bc8e0b$f1a8fea0$8371d8cb@thinkpad> NNTP-Posting-Host: tc-1-123.osaka.gol.com X-Newsreader: Microsoft Internet News 4.70.1157 I'm posting this message for a friend of mine. He is a Japanese Professor at Osaka University of Health and sports Sciences. He wishes to do a sabbatical year, starting April 1st 1998, working on the effects of walking on the lipoprotein metabolism of vascular endothelium in dietary rats. He would like to study molecular biology and wishes to obtain a place in a laboratory, in Australia, at Japan's expense. If anybody could supply any information about Universities in Australia dealing in these areas or is able to make any suggestions, then I would be most grateful. Please reply directly to sully@gol.com. Thank you. From owner-rapd@net.bio.net Sun Jul 13 23:00:00 1997 Path: biosci!NEELIX.UDAYTON.EDU!benbow From: benbow@NEELIX.UDAYTON.EDU ("M. Eric Benbow") Newsgroups: bionet.molbio.rapd Subject: (none) Date: 14 Jul 1997 06:08:53 -0700 Organization: University of Dayton Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <1F88D056B26@neelix.udayton.edu> NNTP-Posting-Host: net.bio.net unsubscribe From owner-rapd@net.bio.net Mon Jul 14 23:00:00 1997 Path: biosci!AGRICULTURE.UCD.IE!CSCOTT From: CSCOTT@AGRICULTURE.UCD.IE Newsgroups: bionet.molbio.rapd Subject: SCORING RAPDS Date: 15 Jul 1997 10:34:25 -0700 Organization: University College Dublin Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <891360C1383@agriculture.ucd.ie> NNTP-Posting-Host: net.bio.net DEAR RAPD I have used RAPDS on Fungi. I have calulated my similarity and distance matrices and now wish to know which is the best programme for cluster analysis? where can I download it? Thanks for your help. Carol. From owner-rapd@net.bio.net Wed Jul 16 23:00:00 1997 Path: biosci!byu.edu!JLFarmer From: JLFarmer@byu.edu (James Farmer) Newsgroups: bionet.molbio.rapd Subject: Charter for the rapd newsgroup Date: 17 Jul 1997 14:56:32 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 68 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.1.32.19970717155844.006a43ac@ucspop.byu.edu> NNTP-Posting-Host: net.bio.net Due to the large number of commercial postings, we will begin moderating this newsgroup very soon. I have four volunteers to help with the duties. BIOSCI requires us to submit a charter document as part of this process. Since this newsgroup already exists, this is largely a formality, but nonetheless I would like the charter to be a respectable document. A copy of the proposed charter, which is in the format suggested by BIOSCI, follows this message. If you have suggestions, please send them to me by Monday July 21. I am especially interested in comments about the part of the charter which refers to commercial postings of interest to people like us. Thanks. Jim Farmer ================================================= USENET newsgroup name: bionet.molbio.rapd Status: Moderated One line Description: Research using RAPD (randomly amplified polymorphic DNA) Moderation address: bionet-molbio-rapd@net.bio.net Moderators: Christopher J.S. Bolch, James L. Farmer, Alastair Hamilton, Andreas Matern Mailing list name: RAPD E-mail addresses: rapd@net.bio.net rapd@daresbury.ac.uk Newsgroup Charter: The bionet.molbio.rapd forum is a site for scientific discussion and exchange of information about randomly amplified polymorphic DNA (RAPD) in biological research (e.g., such as genetic mapping and estimating genetic diversity). Functions of the newsgroup: The newsgroup will allow investigators to exchange information on primers, amplification conditions, evaluations of analytical tools, statistical analysis of RAPD data, technical problems, publications, species which are being analyzed using RAPD, or any other aspect of work relating to this technique. The newsgroup will serve as a bulletin board for announcements of meetings, conferences, job opportunities, and funding sources which could reasonably be expected to be of interest to persons who use RAPD. All persons who have a professional interest in RAPD are encouraged to participate. Moderation Policy: Mass-posted commercial messages, chain letters, and similar postings will be deleted. Inappropriate messages posted in good faith will be returned to the sender. Messages not strictly within the charter but likely to be of interest to many subscribers (e.g., messages dealing with PCR primers that might be of interest to persons who work with RAPD) will be accepted. Use of the newsgroup for commercial purposes is generally prohibited, although announcements (e.g., new equipment, new enzyme sources, new primer sources) which may be of great interest to persons who work with RAPD will be considered if they contain only a very short description of the item or service and an internet or email address where interested parties may obtain more information. DISCUSSION LEADER James L. Farmer 801-378-2153 Dept. of Zoology 801-378-7423 (FAX) Brigham Young University JLFarmer@byu.edu Provo, Utah 84602, USA From owner-rapd@net.bio.net Wed Jul 16 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Jul 1997 02:00:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199707170900.CAA15341@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-rapd@net.bio.net Sun Jul 20 23:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!news.uh.edu!bunews.baylor.edu!chris-kearney.baylor.edu!user From: Steven_Burghart@baylor.edu (Baylor Molecular Genetics Lab) Newsgroups: bionet.molbio.rapd Subject: Re: SCORING RAPDS Date: 21 Jul 1997 22:52:37 GMT Organization: Baylor University Lines: 25 Distribution: world Message-ID: References: <891360C1383@agriculture.ucd.ie> NNTP-Posting-Host: chris-kearney.baylor.edu Probably there are plenty commercial software for cluster analysis. For free you could go to yahoo and type PHYLIP. Might be you will find something appropriate. I am not an expert but hope this will help. Sincerely, Yerlan In article <891360C1383@agriculture.ucd.ie>, CSCOTT@AGRICULTURE.UCD.IE wrote: > DEAR RAPD > > I have used RAPDS on Fungi. I have calulated my similarity and distance > matrices and now wish to know which is the best programme for cluster > analysis? where can I download it? > > Thanks for your help. > > Carol. -- Baylor Molecular Genetics Laboratory (817) 755-1050 http://diogenes.baylor.edu/WWWproviders/molecular_genetics/home.html From owner-rapd@net.bio.net Sun Jul 20 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!newsfeed.direct.ca!news-sea-19.sprintlink.net!news-in-west.sprintlink.net!news.sprintlink.net!Sprint!138.5.100.92!servers!news From: "Steve Carleton, Ph.D" Newsgroups: bionet.molbio.rapd Subject: mRNA fingerprinting Date: Mon, 21 Jul 1997 13:11:31 -0500 Organization: SUNY Health Science Center, Brooklyn Lines: 3 Message-ID: <33D3A653.433B@netmail.hscbklyn.edu> NNTP-Posting-Host: 138.5.46.80 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.02 (Macintosh; I; PPC) Has anyone used the HIEROGLYPH mRNA system ? We are wondering if we should use this (or another) system, or just put together our own primers for our mRNA fingerprinting study on rat brain. From owner-rapd@net.bio.net Wed Jul 23 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!spool.mu.edu!uwm.edu!vixen.cso.uiuc.edu!news-peer.sprintlink.net!news.sprintlink.net!Sprint!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!News1.Ottawa.iSTAR.net!news.istar.net!webster.srv.gc.ca!news.agr.ca!NewsWatcher!user From: hachey@em.agr.ca (John E. Hachey) Newsgroups: bionet.molbio.rapd Subject: rapd primer lists Date: Thu, 24 Jul 1997 14:32:02 -0700 Organization: Agriculture and Agri-Food Canada Lines: 20 Message-ID: NNTP-Posting-Host: dmzgate.agr.ca Hello, Is there available a text list of available 10-mer primers that one could use for searching for specific sequences and finding out which supplier makes it? Thanks, John H. -- ------------------------------------------------------------------ | John E. Hachey * Telephone : (403)327-4561 | | Agriculture and Agri-Food Canada * Fax : (403)382-3156 | | Research Station * Email : hachey@abrsle.agr.ca| | P.O. Box 3000, * Voicemail: (403)327-4591 #658| | Lethbridge, AB * Everything in Moderation, | | Canada * Including Moderation | | T1J 4B1 * | | | |******************************************************************| | DISCLAIMER : BUT THEN AGAIN, I LIKED HEE HAW | | | ------------------------------------------------------------------ From owner-rapd@net.bio.net Thu Jul 24 23:00:00 1997 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!EU.net!Norway.EU.net!uninett.no!due.unit.no!not-for-mail From: Hans Stenöien Newsgroups: bionet.molbio.rapd Subject: RAPD/infinite allele model Date: Fri, 25 Jul 1997 08:49:47 +0200 Organization: The Norwegian University of Science and Technology Lines: 28 Message-ID: <33D84C8B.185B@vm.unit.no> NNTP-Posting-Host: v-a-101.vm.ntnu.no Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 3.0Gold (Win95; I) Hi' I'm doing a population genetic analysis on selected mosses using RAPD and allozyme markers. In an ideal world I would be interested in using Nei's (1978) genetic distance D in order to estimate the mutation rate u given a specific time t since divergence for two populations (D=2ut; that is: u=D/2t, see e.g. Nei 1987 or Weir 1996). One of the assumptions in this model is that all new mutations are different from extant alleles in the populations (the infinite alleles mutation model). This does not look like an appropriate genetic model for interpreting RAPD bands, because each locus per definition contains no more than 2 alleles. However, the infinite alleles model have been assumed in several studies using allozyme markers. Can anybody tell me why it is reasonable to assume that all new mutations in amino-acid coding sequences leads to qualitatively new allozyme alleles? When allozymes can be interpreted as "infinite alleles", are there also ways to fit the infinite alleles model to RAPD data? I would appreciate any help. Thank you. Hans Stenøien Botanical dept. Norwegian University of Science & Technology N-7004 Trondheim Norway hans.stenoien@vm.ntnu.no From owner-rapd@net.bio.net Thu Jul 24 23:00:00 1997 Path: biosci!hboi.edu!lopez From: lopez@hboi.edu (Jose Lopez) Newsgroups: bionet.molbio.rapd Subject: Use of AFLP Trademark Date: 25 Jul 1997 14:17:46 -0700 Organization: Harbor Branch Oceanographic Institution Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: <33D8E1AF.7907@hboi.edu> NNTP-Posting-Host: net.bio.net Dear Colleagues, We are currently applying the AFLP method for developing species specific markers of various marine invertebrates, using the method described by Mueller et al (Mol Ecol 5:119-122, 1996; also Lopez and Knowlton, 8th Intl Coral Reef Symp, Panama). This method is a non-radioactive, agarose gel modification of the original Zabeau and Vos method, which runs the labeled markers on polyacrylamide gels. My question doesn't relate so much to the science of AFLPs, but as to the appropriate and legal use of the AFLP trademark, about which I admit complete naivete. I know that Keygene holds the patent for AFLPs, so does this mean that everyone has to include the "TM" whenever the word AFLP is used on a page or in a publication? (I have not seen this done in every case) Also, would the modified technique of Mueller et al also be subject to the same restrictions (if any) of the trademark and patent? In general what is the purpose of TM for any name? Any clarification of these questions is appreciated. Joe Lopez, Ph.D. Harbor Branch Oceanographic Institution Ft. Pierce FL, 34946 Lopez@hboi.edu 561-466-1552 From owner-rapd@net.bio.net Sun Jul 27 23:00:00 1997 Path: biosci!cabi.org!D.BRAYFORD From: D.BRAYFORD@cabi.org ("David Brayford ", IMI) Newsgroups: bionet.molbio.rapd Subject: RE: rapd primer lists Date: 28 Jul 1997 04:43:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Distribution: world Message-ID: <33DC857B@msm.cgnet.com> NNTP-Posting-Host: net.bio.net Check out the Dendrome Project page at: http://s27w007.pswfs.gov/Data/primer.html This provides gopher links allowing you to download primer sequences from (if I remember correctly) Operon, Genosys and UBC RAPDs sets. Dave Brayford, IMI d.brayford@cabi.org ---------- From: hachey@em.agr.ca To: rapd@net.bio.net Subject: rapd primer lists Date: 24 July 1997 2:32 Hello, Is there available a text list of available 10-mer primers that one could use for searching for specific sequences and finding out which supplier makes it? Thanks, John H. -- ------------------------------------------------------------------ | John E. Hachey * Telephone : (403)327-4561 | | Agriculture and Agri-Food Canada * Fax : (403)382-3156 | | Research Station * Email : hachey@abrsle.agr.ca| | P.O. Box 3000, * Voicemail: (403)327-4591 #658| | Lethbridge, AB * Everything in Moderation, | | Canada * Including Moderation | | T1J 4B1 * | | | |******************************************************************| | DISCLAIMER : BUT THEN AGAIN, I LIKED HEE HAW | | | ------------------------------------------------------------------ From owner-rapd@net.bio.net Sun Jul 27 23:00:00 1997 Path: biosci!FORUM.VA.GOV!FISHER.CYNTHIA From: FISHER.CYNTHIA@FORUM.VA.GOV Newsgroups: bionet.molbio.rapd Subject: RAPD Date: 28 Jul 1997 13:49:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <24582714@FORUM.VA.GOV> NNTP-Posting-Host: net.bio.net I would like to belong to the rapd@net. I believe that you have the RAPD-L archives as well as the rapd@net.bio.net archives. I have had trouble obtaining the expected fingerprinting bands for Mycobacterium tuberculosis...now I sm getting a featureless smuge that doesn't migrate a great distance. James Farmer suggested that I may have too much DNA in the process..this week's challenge. Can anyone help me with this problem? Also, how do I go about receiving archives. Thank you. Fisher, Cynthia_B@Bay-Pines.VA.Gov. From owner-rapd@net.bio.net Tue Jul 29 23:00:00 1997 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!howland.erols.net!torn!news.dal.ca!newsflash.concordia.ca!feed.umontreal.ca!alize.ERE.UMontreal.CA!landryp From: landryp@ERE.UMontreal.CA (Landry Pierre-Alexandre) Newsgroups: bionet.molbio.rapd Subject: Re: RAPD Date: 30 Jul 97 15:38:11 GMT Organization: Universite de Montreal Lines: 20 Distribution: world Message-ID: References: <24582714@FORUM.VA.GOV> NNTP-Posting-Host: alize.ere.umontreal.ca FISHER.CYNTHIA@FORUM.VA.GOV writes: >I would like to belong to the rapd@net. I believe that you have >the RAPD-L archives as well as the rapd@net.bio.net archives. >I have had trouble obtaining the expected fingerprinting bands >for Mycobacterium tuberculosis...now I sm getting a featureless >smuge that doesn't migrate a great distance. James Farmer >suggested that I may have too much DNA in the process..this >week's challenge. Can anyone help me with this problem? >Also, how do I go about receiving archives. Thank you. >Fisher, Cynthia_B@Bay-Pines.VA.Gov. I'd agree that you have too much DNA. One of the most important factor in the reaction is the quality and quantity of DNA. I never put more than 1ng of DNA per microli of reaction, i.e. 25 ng in 25 microli reactions. Best, Ax From owner-rapd@net.bio.net Thu Jul 31 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!news.maxwell.syr.edu!infeed1.internetmci.com!newsfeed.internetmci.com!compuserve.com!news.production.compuserve.com!news From: Mike Mounfield <100555.2502@CompuServe.COM> Newsgroups: bionet.molbio.rapd Subject: Preservation of DNA Material Date: 1 Aug 1997 15:36:55 GMT Organization: Cheshire County Council Lines: 15 Message-ID: <5rsvqn$ec9$1@mhafn.production.compuserve.com> Please excuse my intrusion into your ng. I need advice on how to preserve tissue from a recently dead animal for later analysis. How quickly does DNA break down? What measures can I take to prevent/stall this? The tissue is fur and I'm an engineer not a biomedical person.  Many thanks.  Mike Mounfield -- Road Safety Engineer Cheshire CC have their own views and these ain't necessarily the same. "Prejudice is the reason of fools" - Voltaire