From owner-rapd@net.bio.net Sun Aug 03 23:00:00 1997 Path: biosci!RSBS.ANU.EDU.AU!JAGADISH From: JAGADISH@RSBS.ANU.EDU.AU ("VIDYA JAGADISH") Newsgroups: bionet.molbio.rapd Subject: (none) Date: 4 Aug 1997 16:16:24 -0700 Organization: RSBS, Australian National Uni. Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <1393650420F@rsbs.anu.edu.au> Reply-To: Jagadish@rsbs.anu.edu.au NNTP-Posting-Host: net.bio.net unsubscribe rapd From owner-rapd@net.bio.net Mon Aug 04 23:00:00 1997 Path: biosci!RSBS.ANU.EDU.AU!SKOTNICKI From: SKOTNICKI@RSBS.ANU.EDU.AU ("MARY SKOTNICKI") Newsgroups: bionet.molbio.rapd Subject: Primers for unknown plants Date: 4 Aug 1997 17:57:02 -0700 Organization: RSBS, Australian National Uni. Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <13AE47E7C1C@rsbs.anu.edu.au> NNTP-Posting-Host: net.bio.net I've been asked to help identify some tiny (dead) dicot seedlings, by comparison with 15 very different but known house plant species (but I also only have dead seedlings) from the same remote location. As there is only a minute amount of material available, I can't afford to do primer trials, although RAPDs seem the best way to start as I'm thoroughly familiar with the techniques. Therefore, can anyone suggest some good primers suitable for a range of dicot plants? Preferably Operon primers, as I have lots of those. I'd appreciate any help! Thanks, Mary Skotnicki Dr Mary Skotnicki Photobioenergetics Research School of Biological Sciences Australian National University Canberra ACT 0200 Australia SKOTNICKI@RSBS-CENTRAL.ANU.EDU.AU Tel +61 6 2492386 or 2495894 Fax +61 6 2798056 From owner-rapd@net.bio.net Wed Aug 06 23:00:00 1997 Path: biosci!POPHOST.EUNET.BE!RvP From: RvP@POPHOST.EUNET.BE (eu000497) Newsgroups: bionet.molbio.rapd Subject: SAMPL-protocol Date: 7 Aug 1997 02:41:26 -0700 Organization: RvP Lines: 11 Sender: daemon@net.bio.net Distribution: world Message-ID: <33E99964.2DAD@pophost.eunet.be> Reply-To: RvP@pophost.eunet.be NNTP-Posting-Host: net.bio.net Dear newsgroupers, I' am looking for more information about the SAMPL-technique ! Can someone help me ? Thanks, Jochen Dendauw E-mail : rvp@pophost.eunet.be From owner-rapd@net.bio.net Mon Aug 11 23:00:00 1997 Path: biosci!bloom-beacon.mit.edu!panix!howland.erols.net!vixen.cso.uiuc.edu!news.indiana.edu!pumpkin.bio.indiana.edu!user From: dewolf@bio.indiana.edu (Diana Wolf) Newsgroups: bionet.molbio.rapd Subject: Re: Primers for unknown plants Date: Mon, 11 Aug 1997 20:22:27 -0600 Organization: Indiana University Lines: 48 Distribution: world Message-ID: References: <13AE47E7C1C@rsbs.anu.edu.au> NNTP-Posting-Host: pumpkin.bio.indiana.edu My advisor puplished a survey of several hundred Operon and UBC primers, and how well they amplified DNA from 3 completely unrelated species - Datisca, Yucca and Helianthus. You can just pick the primers that amplified well in all three species. The reference is: Fritsch et al. 1993. Constancy of RAPD Primer Amplification Strength amon Distantly Related Taxa of Flowering Plants. Plant Molecular Biology Reporter. 11(1): 10-20. If you don't have access to this journal, let me know and I will mail a copy of the paper to you. Diana In article <13AE47E7C1C@rsbs.anu.edu.au>, SKOTNICKI@RSBS.ANU.EDU.AU ("MARY SKOTNICKI") wrote: > I've been asked to help identify some tiny (dead) dicot seedlings, by > comparison with 15 very different but known house plant species (but > I also only have dead seedlings) from the same remote location. As > there is only a minute amount of material available, I can't afford > to do primer trials, although RAPDs seem the best way to start as > I'm thoroughly familiar with the techniques. > > Therefore, can anyone suggest some good primers suitable for a range > of dicot plants? Preferably Operon primers, as I have lots of those. > I'd appreciate any help! > > Thanks, > Mary Skotnicki > Dr Mary Skotnicki > Photobioenergetics > Research School of Biological Sciences > Australian National University > Canberra > ACT 0200 > Australia > > SKOTNICKI@RSBS-CENTRAL.ANU.EDU.AU > > Tel +61 6 2492386 or 2495894 > Fax +61 6 2798056 -- Diana Wolf Biology Department Indiana University From owner-rapd@net.bio.net Sat Aug 16 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Aug 1997 02:00:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199708170900.CAA08028@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-rapd@net.bio.net Sat Aug 16 23:00:00 1997 Path: biosci!nus.edu.sg!sbsrjh From: sbsrjh@nus.edu.sg (Ric) Newsgroups: bionet.molbio.rapd Subject: RE: Preservation of material Date: 17 Aug 1997 05:06:25 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <33F7C63E.7369@nus.edu.sg> Reply-To: sbsrjh@nus.edu.sg NNTP-Posting-Host: net.bio.net We have a paper coming out this month in BioTechniques which covers the suitability of different tissue storage and preservation protocols for later DNA extraction. The suitability for RAPD is also tested. We also suggest a new protocol. Dr James Nelson School of Biological Sciences National University of Singapore Singapore sbsjsn@nus.edu.sg From owner-rapd@net.bio.net Tue Aug 19 23:00:00 1997 Path: biosci!daresbury!uninett.no!news-feed.inet.tele.dk!newsfeed.direct.ca!news.he.net!news.pagesat.net!news.itis.com!news.doit.wisc.edu!news From: Mark Molenda Newsgroups: bionet.molbio.rapd Subject: New Biotech Company Specializing in Paper Accessories & Printers/Cameras Date: 20 Aug 1997 02:46:45 GMT Organization: UW-Madison Lines: 10 Message-ID: <5tdlql$hr4@news.doit.wisc.edu> NNTP-Posting-Host: wn067-033.wiscnet.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.22 (Windows; I; 16bit) Scientific Digital Imaging is a new company specializing in accessories for the life sciences and medical imaging products. Presently SDI specializes in ALL forms of paper for thermal and dye sub printers being used in imaging systems. The prices for Mitsubishi, Sony, Toshiba and many more paper is 25-50% off retail. SDI also carries cameras and printers at 25-50% off. They can be contacted at 414-476-2694 or at scidi@juno.com. From owner-rapd@net.bio.net Sun Aug 24 23:00:00 1997 From: Mitch Frederick Newsgroups: bionet.molbio.rapd Subject: Reverse northerns Date: Mon, 18 Aug 1997 23:18:26 -0500 Organization: World Trade Network, Inc. Lines: 3 Message-ID: <33F91E92.38DB@wt.net> NNTP-Posting-Host: wt-d4-68.wt.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win95; I) Path: biosci!agate!howland.erols.net!infeed1.internetmci.com!newsfeed.internetmci.com!192.48.96.123!in1.uu.net!205.230.159.11!news1.wt.net!news3.wt.net!not-for-mail Has anyone had great sucess with reverse northerns for confirming differential expression of genes? Does it agree well with conventional northern data? From owner-rapd@net.bio.net Sun Aug 24 23:00:00 1997 From: Mitch Frederick Newsgroups: bionet.molbio.rapd Subject: Reverse Northerns Date: Mon, 18 Aug 1997 23:06:35 -0500 Organization: World Trade Network, Inc. Lines: 3 Message-ID: <33F91BCB.2EE7@wt.net> NNTP-Posting-Host: wt-d4-68.wt.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Win95; I) Path: biosci!agate!hammer.uoregon.edu!news.oru.edu!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!infeed1.internetmci.com!newsfeed.internetmci.com!192.48.96.123!in1.uu.net!205.230.159.11!news1.wt.net!news3.wt.net!not-for-mail Has anyone had great sucess with reverse northerns for confirming differential expression of gene products? How well does it agree with regular northerns ? From owner-rapd@net.bio.net Sun Aug 24 23:00:00 1997 Path: biosci!SCHERAGO.COM!info From: info@SCHERAGO.COM (Scherago International, by way of C. S. Prakash) Newsgroups: bionet.molbio.rapd Subject: American Genetic Resources Alliance Date: 25 Aug 1997 11:47:16 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 90 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net >X-POP3-Rcpt: info@golem >From: AnneMarieR@aol.com >Date: Fri, 15 Aug 1997 18:15:48 -0400 (EDT) >To: pag@scherago.com >Subject: American Genetic Resources Alliance > > >American Genetic Resources Alliance >http://www.amgra.org > >Dear Plant and Animal Genome Conference participant, > >We are writing to urge you to join us in taking action to ensure the >conservation of one of our most fundamental resources; plant genetic >resources for food and agriculture. > >The applied use of genomic science as well as food, health, and > environmental security are inextricably dependent upon the future >availability of useful plant genetic diversity. Yet, this diversity is not >being sufficiently safeguarded by this nation for future generations. > >~Who We Are~ >We are a group of individuals, companies, and non profit organizations from >diverse backgrounds who are concerned about plant genetic resources. > Specifically, we are working to ensure their continued conservation in order >to prevent the further loss of potentially useful genes. This commitment to >strengthen plant genetic resource conservation unites us despite differences >of opinion we may have about other facets of agriculture and horticulture. > >~What We Are Doing~ >WE ARE NOT SEEKING FUNDING OR DONATIONS. We are seeking to build alliances >with others who are concerned about food and health issues, agriculture, >horticulture and environmental quality. Together we are working to build >public and Congressional awareness about the imperative need to conserve >plant genetic resources in order to help meet current and future food needs. > > * It is essential that we, as a nation, take serious and effective measures >to ensure the continued conservation and availability of plant genetic >resources, otherwise research opportunities will be lost and the health and >prosperity of current and future generations will be compromised. * > >The Publicly funded National Plant Germplasm System is one of the most >effective ways plant genetic resources for many crops, especially field >crops, can be conserved. However this program requires sufficient funding, >which is substantially more than is currently allocated by Congress. > Increased funding will be approved only when Congress is made aware of the >fundamental need for plant genetic resource conservation by overwhelming >public support. > >~Please Join Us~ >We would like you to join us in our efforts to support plant genetic resource >conservation for the benefit of all. WE ARE NOT ASKING FOR DONATIONS OR >FUNDING. WE ARE asking you to make a contribution by: > > *Becoming an ally by using our website (http://www.amgra.org) or >email (annemarier@aol.com) to reply with your name and/or your organization's >name, to be included among those supporting strengthened plant genetic >resource conservation. > > *Informing your colleagues or your organization's membership about >plant genetic resource conservation, the NPGS and the work of the American >Genetic Resources Alliance to support this critically important conservation >work; and > > *Contacting your US Senator and/or Representative to voice your >concerns about and support for the NPGS. > >Thank you >We appreciate your time, consideration and foresight. > Sincerely, > Anne Marie Ruff > >American Genetic Resources Alliance >2212 Griffith Park Blvd. >Los Angeles, CA 90039 >213-913-2507 >http://www.amgra.org > >For more information about our mission, our members and allies, the >importance of genetic resources or to link your website to ours, please visit >our website or email annemarier@aol.com > > ================================================================ Darrin Scherago, CMP Tel: 212-643-1750 Ext. 20 Scherago International Fax: 212-643-1758/57 11 Penn Plaza, Suite 1003 E-mail: darrins@scherago.com New York, NY 10001 WWW: http:\\www.scherago.com From owner-rapd@net.bio.net Mon Aug 25 23:00:00 1997 Path: biosci!agate!newsgate.duke.edu!solaris.cc.vt.edu!hp.fciencias.unam.mx!not-for-mail From: Adriana Bañuelos Pánuco Newsgroups: bionet.molbio.rapd Subject: cells contamination Date: Tue, 26 Aug 1997 17:40:44 -0400 Organization: A poorly-installed InterNetNews site Lines: 7 Message-ID: <34034D5C.C4E@lambda.gene.cinvestav.mx> NNTP-Posting-Host: ribozyme.gene.cinvestav.mx Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (X11; I; SunOS 5.5 sun4m) I`m a doctorate student. I`m going to work whit novel cervical cancer cells lines. I want to study the state of some cellular genes, but there is a strong suspicion that this lines cells are contaminated with HeLa cells. How can I probe this possible contamination?. I don`t know any specific characteristic of HeLa that could helps me. Adriana Banuelos From owner-rapd@net.bio.net Thu Aug 28 23:00:00 1997 Path: biosci!PUBLIC.EAST.CN.NET!lbwei From: lbwei@PUBLIC.EAST.CN.NET Newsgroups: bionet.molbio.rapd Subject: (none) Date: 28 Aug 1997 17:55:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <199708290055.RAA02377@net.bio.net> NNTP-Posting-Host: net.bio.net subscribe RAPDinformation