From owner-rapd@net.bio.net Sat Nov 01 22:00:00 1997 Path: biosci!agate!howland.erols.net!news.maxwell.syr.edu!newsfeed.gte.net!news.pn.com!nntp.pn.com!mozo.cc.purdue.edu!purdue!haven.umd.edu!news.umbc.edu!kcarter From: kcarter@umbc.edu (Mr. Ken Carter) Newsgroups: bionet.molbio.rapd Subject: DNA Fingerprinting Workshop Date: 28 Oct 1997 15:00:32 GMT Organization: University of Maryland, Baltimore County Lines: 62 Message-ID: <634umg$9o9$5@news.umbc.edu> NNTP-Posting-Host: umbc7.umbc.edu X-Newsreader: TIN [version 1.2 PL2] MICROBIAL DNA FINGERPRINTING November 4-7, 1997 American Type Culture Collection (ATCC), Rockville, MD DNA fingerprinting is an extremely useful tool in the fields of clinical and industrial microbiology as well as epidemiology. This four-day, laboratory-intensive, course is designed to introduce the participant to both manual and automated methods for fingerprinting microbial genomes. The microorganisms examined will include eukaryotes and bacteria. The workshop will provide introductory and intermediate level instruction, not only in the currently available technologies, but also in the different options for data analysis. A general background in molecular biology and PCR* applications is helpful, but not necessary. approximately 75% of the course is devoted to laboratory instruction, while 25% is lecture-oriented. An extensive manual with protocols, procedures and trouble-shooting tips will be provided to each participant. Lecture topics will include: 1.DNA isolation and purification, 2.General options for microbial DNA fingerprinting, 3.PCR-based fingerprinting methods (e.g., PCR with arbitrary primers, inter-repeat PCR, fingerprinting by amplification of tRNA / rRNA spacers, and selective restriction fragment amplification, 4.Automated fingerprinting methods, 5.Troubleshooting, and 6.Data analysis. Results of hands-on experiments will be analyzed and discussed. There will be ample opportunity for consultations with workshop faculty on the various options available for microbial DNA fingerprinting. Faculty: Jane Tang, Ph.D., Collection Scientist (ATCC); Maria Saluta, Ph.D. (Pharmacia Biotech); Tom Nerad, Ph.D., Collection Scientist (ATCC); Roberto Rodriguez, Ph.D. (Pharmacia Biotech); Dennis Devitt, Ph.D. (Pharmacia Biotech). Limited to 25 participants FEE: $1,195.00 2.8 CEUs * The polymerase chain reaction (PCR) process is covered by patents owned by Hoffmann-La Roche. Use of the PCR process requires a license. **For a full schedule and on-line registration, please visit our Web site at: http://www.atcc.org/workshops/workshop.html **Or request a brochure, which includes a full schedule and registration form from: kcarter@atcc.org or call 1-800-359-7370 -- ****************************************************************************** Ken Carter ^ American Type Culture Collection / | \ (301) 231-5525 12301 Parklawn Drive /___|___\__ fax:(301) 816-4364 Rockville, Maryland 20852 \______/ kcarter@atcc.org ~~~~~~~~~~~~~~ From owner-rapd@net.bio.net Tue Nov 04 22:00:00 1997 Path: biosci!LAMAR.COLOSTATE.EDU!antolin From: antolin@LAMAR.COLOSTATE.EDU (Michael Antolin) Newsgroups: bionet.molbio.rapd Subject: Evolution position Date: 5 Nov 1997 11:30:27 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 61 Sender: daemon@net.bio.net Distribution: world Message-ID: <199711051929.MAA71166@lamar.ColoState.EDU> NNTP-Posting-Host: net.bio.net > ------------- > POSITION ANNOUNCEMENT > > MECHANISMS OF EVOLUTION IN ANIMAL POPULATIONS > > POSITION: Assistant Professor, Department of Biology, Colorado State > University with expertise in the mechanisms of evolution in natural > populations of animals. We are particularly interested in candidates who > use modern techniques such as quantitative genetics and molecular > approaches as part of their experimental programs. Areas of inquiry may > include parasite-host coevolution, life history evolution, and genetics of > speciation. This tenure-track position involves undergraduate and graduate > teaching and supervised research (approximately fifty percent), research > (approximately thirty-five percent), and service/outreach (approximately > fifteen percent). > > RESPONSIBILITIES: This position involves a strong teaching commitment, > including a course in Introductory Biology/Zoology and advanced evolution > courses, both undergraduate and graduate, in the successful candidate's > area of expertise. The successful candidate will be expected to develop an > independent, externally-funded research program in evolutionary biology, > and to contribute to interdisciplinary programs on campus. > > QUALIFICATIONS: A Ph.D. in zoology or a related area is required. > Postdoctoral experience is strongly preferred. Candidates should have a > solid background in evolutionary biology, potential for independent and > innovative research and teaching, and an ability to contribute to > interdisciplinary programs on campus. > > SALARY: Commensurate with education and experience. > > POSITION AVAILABLE: Fall semester, 1998. > > To apply, send a letter of application with a statement of your teaching > and research interests, a curriculum vitae, and no more than three > publications; also arrange to have letters sent from at least three persons > who will serve as references to: > > Evolution Search Committee > Department of Biology > Colorado State University > Fort Collins CO 80523 > Telephone: (970) 491-7011 > FAX (970) 491-0649 > email botteron@lamar.colostate.edu > > All materials are due by 10 December 1997. The search may be extended if > suitable candidates are not found. > > *** *** *** *** *** *** *** *** *** *** > > Colorado State University is an equal opportunity/affirmative action > employer and complies with all Federal and Colorado State laws, > regulations, and executive orders regarding affirmative action > requirements. The Office of Equal Opportunity is located in 101 Student > Services. In order to assist Colorado State University in meeting its > affirmative action responsibilities, ethnic minorities, women, and other > protected class members are encouraged to apply and to so identify > themselves. > From owner-rapd@net.bio.net Tue Nov 04 22:00:00 1997 Path: biosci!LAZARLAB.COM!service From: service@LAZARLAB.COM Newsgroups: bionet.molbio.rapd Subject: Measure pH of very small bio sample Date: 5 Nov 1997 13:57:21 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <199711051056.MAA27701@alpha.netvision.net.il> NNTP-Posting-Host: net.bio.net Novel Research Tool measures pH of very small Biological samples A micro pH electrode has been developed which can measure samples as small as 5 microliters which is less than 1/10 the volume of a single drop of liquid. It can also measure small biological specimens down to less than 0.5 mm in length. The electrode fits directly into 96 well plates, microcentrifuge tubes, serum cups, capillary tubes, and NMR tubes. Biological specimens which can be measured include various animal and plant tissues. Surface pH measurements on biological samples can also be made. The probe is all solid state with a Teflon body making it virtually unbreakable. It has a 1 mm tip and 2 mm body and can be used with any standard pH meter or strip chart recorder. For further details email service@lazarlab.com or fax 1-213-931-1434. Micro probes are also available to measure ions such as Na, Cl, and K as well as pO2 and pCO2. Sincerely, Lazar Research Laboratories, Inc. R. Chan, Ph.D. From owner-rapd@net.bio.net Thu Nov 06 22:00:00 1997 Path: biosci!agate!newsgate.duke.edu!news.eng.convex.com!news.ecn.uoknor.edu!paladin.american.edu!newscore.univie.ac.at!news-ge.switch.ch!news.rediris.es!news.uv.es!not-for-mail From: cmariac@post.uv.es Newsgroups: bionet.molbio.rapd Subject: Help needed Date: 7 Nov 1997 11:44:36 GMT Organization: Universitat de Valencia, Spain Lines: 9 Message-ID: NNTP-Posting-Host: pcsb3.scsi.uv.es Mime-Version: 1.0 Content-Type: Text/Plain; charset=US-ASCII X-Newsreader: Winbox for Windows V3.1.0 (16) This is a young scientist working on RAPDs with an endemic plant from Balear Islands, Spain. I?m having some problems lately with my results, and I would be very interested in meeting people with experience on this subject, in order to exchange some opinions, (and get some help :-) Waiting for your news ! From owner-rapd@net.bio.net Fri Nov 07 22:00:00 1997 Path: biosci!USERS.AFRICAONLINE.CO.KE!wellcome From: wellcome@USERS.AFRICAONLINE.CO.KE ("Wellcome Trust, Nairobi.") Newsgroups: bionet.molbio.rapd Subject: Long PCR primers Date: 8 Nov 1997 05:40:41 -0800 Organization: Wellcome Trust Research Laboratories Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <346505AD.21C0@users.AfricaOnline.Co.Ke> Reply-To: wellcome@users.africaonline.co.ke NNTP-Posting-Host: net.bio.net Dear Colleagues, If there any risk of amplifying a fragment DNA with primer which are 50 to 60 pb. Actually, I want to introduce a enzyme restriction site in these primers so that I can easily detected them in agore or Acrylamide gel. Therefore I do need longer primers. But I wandering about doing a PCR such long oligonucleotides. My regards, -- Wellcome Trust Research Labs, PO Box 43640, Nairobi, Kenya. Tel 254 (2) 725390, Fax 254 (2) 711673 E-mail: wellcome@users.africaonline.co.ke From owner-rapd@net.bio.net Sun Nov 09 22:00:00 1997 Path: biosci!agate!howland.erols.net!news.maxwell.syr.edu!news-feed1.tiac.net!news-master.tiac.net!news@tiac.net From: propdig Newsgroups: bionet.molbio.rapd Subject: National Biotech Register(NatBio) New Service Date: Mon, 10 Nov 1997 15:40:20 -0500 Organization: propdig Lines: 5 Message-ID: <34677134.186A@barryinc.com> Reply-To: propdig@barryinc.com NNTP-Posting-Host: p11.ts7.lowel.ma.tiac.com Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0C-NSCP (Win95; U) NatBio, http://www.barryinc.com/bio is pleased to introduce a new service on our web site. A Biotech Industry Company profile search. Are you looking for information on a particular Biotech company? We probably have it. We also invite you to check out your own company. If you are not list4ed, email us the data and we will add you for free. From owner-rapd@net.bio.net Mon Nov 10 22:00:00 1997 Path: biosci!ACD.TUSK.EDU!Prakash From: Prakash@ACD.TUSK.EDU ("C. S. Prakash") Newsgroups: bionet.molbio.rapd Subject: Postdoc positions at ICGEB for developing country scientists Date: 11 Nov 1997 07:40:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 121 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Check the website for application form and details: http://www.icgeb.trieste.it ICGEB also offers Ph. D. scholarships and short trainings in molecular biology for developing country scientists. ICGEB FELLOWSHIP PROGRAMME * 1998 * Post-Doctoral Fellowships are available at: ICGEB Trieste, Italy, ICGEB New Delhi, India; Selected Italian Institutes Applications are invited from candidates of ICGEB Member States and must be submitted through, and endorsed by, the ICGEB National Scientific Focal Point (see attached list) in the applicant's country of origin CLOSING DATES: 1st Review: 31 December 1997 2nd Review: 30 June 1998 International Centre for Genetic Engineering and Biotechnology Office of the Director (Fellowship Programme) Padriciano 99, 34012 Trieste, Italy http://www.icgeb.trieste.it Tel: +39-40-3757305 Fax: +39-40-226555 Telex: 460396 ICGEBT I E-mail: vincent@icgeb.trieste.it ---------------------------------------------------------------- GENERAL INFORMATION ICGEB Post-doctoral fellowships for 1998 are available to candidates of ICGEB Member States*. Fellowship awards are for one year with the possibility of an extension for a further year. Annual remuneration is in the region of US$13 - 21,000 depending on the location. All applications will be screened by a scientific committee. Qualifications: recent Ph.D or at least 3 years research experience. Fellowships will be awarded on merit and distributed equitably to nationals of ICGEB Member States. Areas of research are:- ICGEB New Delhi * Hepatitis B, C and E; * Molecular Biology of Malaria (P. falciparium, vivax and knowlesi); * Immune Recognition; * Recombinant Gene Products; * Insect Resistance in Plants; * Plant Virus Resistance; * Replication of Plant DNA; * Plant Transformation; ICGEB Trieste * Analysis of Sequence Dependent DNA Bending; * Human Cell DNA Replication; * DNA Enzymology of Human Cells; * Human DNA and RNA Viruses (papilloma, rotaviruses, HIV, HCV); * Human Molecular Genetics; * Molecular Immunology: recombinant antibodies, tumour biology; * Oncogenes and Tumour Suppressors; * Protein Design, Biocomputing, Sequence Databases; * Protein/DNA Recognition: de novo design and random library approaches; * Recombinant Vaccine Development; * Secondary Metabolism of Lignocellulosics; * Yeast Genome Structure and Function; Italian Institutes * Enzymology; * Human Genetics; * Immunology; * Molecular Biology; * Plant Molecular Biology; * Virology; 1. Complete applications (in triplicate) attaching (i) Curriculum Vitae; (ii) English language proficiency certificate; and (iii) Evidence of continuation of present employment upon completion of fellowship (if applicable) must arrive at ICGEB Trieste by the closing dates of 31 December 1997 or 30 June 1998. 2. An additional copy of the application must be submitted to the National Scientific Focal Point in the applicant's country of origin for their endorsement. In the event that the endorsement is not received at ICGEB by the closing date, the application will not be included for review. 3. Three letters of recommendation should be sent to Trieste by the referees directly. 4. Previous applications which have already been evaluated by the ICGEB Scientific Review Panel cannot be reconsidered. 5. Incomplete (or illegible) applications will not be accepted and will be returned to the applicant. 6. Candidates may not apply for fellowships in the country of their origin. 7. Applicants over the age of 35 years will not be considered. * ICGEB Signatory Countries: Afghanistan, Algeria, Argentina, Bangladesh, Bhutan, Brazil, Bulgaria, Chile, China, Colombia, Costa Rica, Croatia, Cuba, Ecuador, Egypt, F.R. Yugoslavia, Hungary, India, Iraq, Italy, Kuwait, Macedonia, Mauritius, Mexico, Morocco, Nigeria, Pakistan, Panama, Peru, Poland, Romania, Russia, Senegal, Slovenia, Sri Lanka, Sudan, Tunisia, Turkey, Uruguay, Venezuela, Viet Nam 07/97rev. ****************** ****************** ****************** ****************** C. S. Prakash, Ph. D. Prakash@acd.tusk.edu Center for Plant Biotechnology Research Ph: (334) 727 8023 College of Agricultural, Env and Nat Sci. Fax:(334) 727 8067 Tuskegee University http://agriculture.tusk.edu/ Tuskegee, AL 36088, USA ****************** ****************** ****************** ****************** From owner-rapd@net.bio.net Mon Nov 10 22:00:00 1997 Path: biosci!ACD.TUSK.EDU!prakash From: prakash@ACD.TUSK.EDU ("C. S. Prakash") Newsgroups: bionet.molbio.rapd Subject: Theoretical and Applied Genetics Online Date: 10 Nov 1997 17:13:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Springer Verlag has its journls now availabel online but at a cost! Print subscribers can download the whole article in the PDF format (which means you get exactly how it appears in the journal including photos and graphs) but I wonder with $600+ per year how many individual subscribers are out there! For most of us at least, we can now look at the Table of Contents and read the abstracts for free. Check it out at http://link.springer.de/ol/lsol/index.htm Among the hundreds of journals, those relevant to us include Plant Cell Reports, Theoretical and Applied Genetics, Planta, Current Genetics, Sexual Plant Reproduction and MCG Molecular and General Genetics . Prakash ************************************************ C. S. Prakash Tuskegee University Center for Plant Biotechnology Research Tuskegee, AL 36088, USA mailto:Prakash@acd.tusk.edu http://agriculture.tusk.edu Phone (334) 727 8023; Fax (334) 727 8067 ************************************************ From owner-rapd@net.bio.net Sun Nov 16 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Nov 1997 02:00:08 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199711171000.CAA05685@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-rapd@net.bio.net Fri Nov 21 22:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!4.1.16.34!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news-peer.sprintlink.net!news-pull.sprintlink.net!news-in-east.sprintlink.net!news.sprintlink.net!Sprint!199.1.13.10!news1.channel1.com!news.pn.com!nntp.pn.com!news-feeder.onramp.net!news.onramp.net!news From: "Cynthia S. Smagula" Newsgroups: bionet.molbio.rapd Subject: BioToolKit Internet Directory Date: Fri, 21 Nov 1997 20:11:29 -0500 Organization: BIOTA Publications Lines: 14 Message-ID: <3476313A.3B7A@onramp.net> Reply-To: biota@onramp.net NNTP-Posting-Host: isdn14-57.dllstx.onramp.net Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Macintosh; I; PPC) The BioToolKit is now hosted by BioSupplyNet at http://www.biosupplynet.com . The BioToolKit, a searchable and browsable database of 400 internet research applications, includes up-to-date links to nucleic acid, genome, and protein databases, online applications for data retrieval and data analysis, and tools for molecular visualization. The BioToolKit is designed for speed, with pulldown menus to provide rapid access to each section. The BioSupplyNet web site is the online companion to the Source Book, a comprehensive directory of molecular biology products and suppliers. The Source Book was originally published by the Cold Spring Harbor Laboratory Press to supply essential information on sources of reagents and supplies to users of the well-known Molecular Cloning Manuals published by CSHLP. From owner-rapd@net.bio.net Sun Nov 23 22:00:00 1997 Path: biosci!THEOFFICE.NET!a_hussein From: a_hussein@THEOFFICE.NET (Abdallah Abu Hussein) Newsgroups: bionet.molbio.rapd Subject: PCR and Microbial Taxonomy Date: 24 Nov 1997 00:04:49 -0800 Organization: Personal Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <3479366C.B8540FC5@theoffice.net> Reply-To: a_hussein@theoffice.net NNTP-Posting-Host: net.bio.net Dear Colleagues, My name is Raghda Zaki, and I'm a lecturer of Microbiology, Faculty of Medicine, Tanta University, EGYPT. I'm a new member to your list. I hope I'd be of any help to the current list members. I'm preparing a review article on PCR uses in Microbial Taxonomy. I've made full MED-Line searches on the subject, but unfortunately I didn't come up with good results. I wonder if you can point me to some resources on the different methods of PCR uses and applications in Microbial Taxonomy, reviews made on the subject ... etc. (whether on-line or in hard copy) Thanks in adavnce for your help. Sincerely, Dr. Raghda PCR and Microbial Taxonomy From owner-rapd@net.bio.net Tue Nov 25 22:00:00 1997 Path: biosci!agate!howland.erols.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!Cabal.CESspool!bofh.vszbr.cz!newscore.univie.ac.at!news-ge.switch.ch!news-zh.switch.ch!elna.ethz.ch!not-for-mail From: Beat Reidy Newsgroups: bionet.molbio.rapd Subject: DNA extraction Date: Wed, 26 Nov 1997 07:28:06 +0100 Organization: ETHZ Lines: 9 Message-ID: <347BC176.259@hosenruck.ipw.agrl.ethz.ch> Reply-To: beat.reidy@hosenruck.ipw.agrl.ethz.ch NNTP-Posting-Host: ipw-cp-234.ethz.ch Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.04Gold (Win95; I) Hi there! Does anyone know a reliable protocol or a reference for DNA extraction from human blood. Thanks Beat -- ---------------------------------------------------- To reply, remove "hosenruck" from the e-mail adress! ---------------------------------------------------- From owner-rapd@net.bio.net Tue Nov 25 22:00:00 1997 Path: biosci!agate!logbridge.uoregon.edu!ais.net!news.maxwell.syr.edu!nntp.news.xara.net!xara.net!server5.netnews.ja.net!daresbury!not-for-mail From: tweissen@hgmp.mrc.ac.uk (Mr. T. Weissensteiner) Newsgroups: bionet.molbio.rapd Subject: >PCR and Microbial Taxonomy Date: 26 Nov 1997 17:13:37 -0000 Lines: 56 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <65hlc1$6cb@mserv1.dl.ac.uk> Original-To: rapd@dl.ac.uk Dear Dr. Raghda I do not know whether you are intending to include technical aspects of RAPD typing in your review. Nevertheless here is a paper on PCR amplification bias caused by differences in the internal amplified sequence (not primers), that I recently wrote and which, I think, could be interesting to you or other members of this newgroup: T. Weissensteiner and J.S. Lanchbury. 1996. Strategy for controlling preferential amplification and avoiding false negatives in PCR typing reactions. Biotechniques 21, pp 1102-8). Email me if you would like photocopies (sorry, no reprints - too expensive these days...:^( ). Regards Thomas Weissensteiner Jenner Institute for Vaccine Research Compton, Berkshire RG20 7NN, U.K. Fax: 0044 1625 577901 Email: tweissenRETURN@hgmp.mrc.ac.uk (please remove capitalized letters to obtain functional address) > From: a_hussein@THEOFFICE.NET (Abdallah Abu Hussein) > Subject: PCR and Microbial Taxonomy > Date: 1997/11/24 > Dear Colleagues, > My name is Raghda Zaki, and I'm a lecturer of Microbiology, Faculty of > Medicine, Tanta University, EGYPT. I'm a new member to your list. > I hope I'd be of any help to the current list members. > I'm preparing a review article on PCR uses in Microbial Taxonomy. > I've made full MED-Line searches on the subject, but unfortunately > I didn't come up with good results. > I wonder if you can point me to some resources on the different methods > of PCR uses and applications in Microbial Taxonomy, reviews made on > the subject ... etc. (whether on-line or in hard copy) > Thanks in adavnce for your help. > Sincerely, > Dr. Raghda From owner-rapd@net.bio.net Wed Nov 26 22:00:00 1997 Path: biosci!agate!howland.erols.net!ais.net!logbridge.uoregon.edu!news.uoregon.edu!newsfeed.orst.edu!129.101.119.221.MISMATCH!news.uidaho.edu!not-for-mail From: Biotechnology Lab Newsgroups: bionet.molbio.rapd Subject: Mapping open-pollinated populations? Date: Wed, 26 Nov 1997 11:40:27 -0800 Organization: University of Idaho Lines: 10 Distribution: bionet Message-ID: <347C7B2B.2F1E@uidaho.edu> NNTP-Posting-Host: biotech.campus.uidaho.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win95; I) Does anyone know if anyone is working on a situation where you have an open-pollinated population from a known mother but the gametophyte is not available? All the progeny have been phenotyped for the trait of interest in replicated trials. There are several half-sib populations available. Any comments on trying to find a marker for a single gene recessive trait would be greatly appreciated. Jeff Schnurr U of I From owner-rapd@net.bio.net Fri Nov 28 22:00:00 1997 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!europa.clark.net!205.252.116.205!howland.erols.net!news.maxwell.syr.edu!newsfeed.eerie.fr!newsfeed.nacamar.de!uni-erlangen.de!cs.tu-berlin.de!map-1.iae.tu-berlin.de!blehn From: Bettina Lehnhardt Newsgroups: bionet.molbio.rapd Subject: AFLP Date: Sat, 29 Nov 1997 16:34:05 +0100 Organization: Technical University of Berlin, Germany Lines: 13 Message-ID: References: <347C7B2B.2F1E@uidaho.edu> Reply-To: Bettina Lehnhardt NNTP-Posting-Host: 130.149.72.27 Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII In-Reply-To: <347C7B2B.2F1E@uidaho.edu> Hi there, I would very much appreciate to get any information on protocols to isolate AFLP fragments from the sequencing gel. Bettina ................. PLEASE REPLY THIS MESSAGE FOR RECEIPT .................... ............................................................................. blehn@map-1.iae.tu-berlin.de ............................................................................. From owner-rapd@net.bio.net Sun Nov 30 22:00:00 1997 Path: biosci!CCVAX.MMC.EDU!swilliams From: swilliams@CCVAX.MMC.EDU Newsgroups: bionet.molbio.rapd Subject: Re: DNA extraction Date: 1 Dec 1997 08:11:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <347BC176.259@hosenruck.ipw.agrl.ethz.ch> NNTP-Posting-Host: net.bio.net We use a kit called Purgene Genomic DNA Isolation Kit from Gentra Systems. It works like a charm, requires next to no equipmewnt and is very fast. The company's address is: Gentra systems 15200 25th Ave., North Suite 104 Mineapolis, MN 55447 1-800-866-3039 Scott Williams Meharry Medical College On Wed, 26 Nov 1997, Beat Reidy wrote: > Hi there! > Does anyone know a reliable protocol or a reference for DNA extraction > from human blood. > Thanks > Beat > -- > ---------------------------------------------------- > To reply, remove "hosenruck" from the e-mail adress! > ---------------------------------------------------- > >