From owner-rapd@net.bio.net Sun Jan 04 22:00:00 1998 Path: biosci!SKYNET.BE!albert.saint.remy From: albert.saint.remy@SKYNET.BE (Albert =?iso-8859-1?Q?Saint=2DR=E9my?=, by way of C. S. Prakash) Newsgroups: bionet.molbio.rapd Subject: Industrial developments in biotechnology Date: 5 Jan 1998 08:42:26 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Colleague, We are organising a European Conference on "Business from genomics" in Turin next March. Recognised by HUGO as a "satellite event" to their HGM'98 conference, it is supported by the industry and the European Commission. Industrial and commercial developments based on research results in genomics will be presented by prominent experts from the industry, the financial milieu and legal affairs specialists. It covers matters such as data bases, intellectual property, venture capital and technology transfer. It includes a show-case for success stories. Check its web site: http:\\www.genomics.org , contact its secretariat for registration:anne.marie.prieels@skynet.be or ask for information about its exhibition: albert.saint.remy@skynet.be . Looking forward to hearing from you, and wishing you a very happy new year =20 Albert Saint-R=E9my =20 From owner-rapd@net.bio.net Thu Jan 08 22:00:00 1998 Path: biosci!APINC.COM!Huang From: Huang@APINC.COM (Ning Huang) Newsgroups: bionet.molbio.rapd Subject: positions avaialble Date: 8 Jan 1998 18:23:21 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net TimesPlant Molecular Biologists Northern California biotech company has two positions immediately available for a Scientist and a Postdoctoral Fellow in the area of transcription control of plant gene expression. Qualifications for the Postdoctoral position include a recent Ph.D. in the field of plant molecular biology or related areas. Qualifications for Scientist include a Ph.D. in plant molecular biology or related area and at least three years research experience in the functional analyses of plant genes and promoters. Applicants are expected to be familiar with standard molecular biology techniques and computer software for DNA sequence analysis. Strong communications and interpersonal skills are required. Salaries are commensurate with the level of experience and accomplishment. Interested applicants should send their curriculum vitae with a list of three references to Human Resources Department, Applied Phytologics, Inc. 4110 N. Freeway Blvd. Sacramento, CA 95834. FAX (916)-921-5611. These positions will be available until filled. ******************** Ning Huang Applied Phytologics INC 4110 N. Freeway Blvd. Sacramento, CA 95834 USA Phone 916-921-6148 FAX 916-921-5611 E-mail Huang@APINC.COM ******************** From owner-rapd@net.bio.net Fri Jan 16 22:00:00 1998 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Jan 1998 02:00:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199801171000.CAA29755@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-rapd@net.bio.net Fri Jan 16 22:00:00 1998 Path: biosci!TUSK.EDU!prakash From: prakash@TUSK.EDU ("C. S. Prakash") Newsgroups: bionet.molbio.rapd Subject: Two Plant Biotechnologists Win the $391,000 Japan Prize! Date: 17 Jan 1998 08:01:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Two pioneering scientists who first developed the transgenic plants in early eighties have won the prestigious and world's richest science award, the Japan Prize. Jozef Schell, 62, of the Max Planck Institute in Cologne, Germany and Marc Van Montagu, 64, of the Flanders Interuniversity Institute of Biotechnology in Ghent, Belgium will share the 50 million yen prize in the category of agricultural biotechnology. The prize is administered by the Science and Technology Foundation of Japan and will be awarded in Tokyo, in April notes the journal SCIENCE (January 9, 1998; p183). On behalf of all plant and agricultural scientists, I congratulate these two giants for their lifetime achievement and contribution to biotechnology and for this prize they so richly deserve! Let us hope that their next stop soon will be Stockholm. ************************************************ C. S. Prakash Tuskegee University Center for Plant Biotechnology Research Tuskegee, AL 36088, USA mailto:Prakash@tusk.edu http://agriculture.tusk.edu Phone (334) 727 8023; Fax (334) 727 8067 ************************************************ From owner-rapd@net.bio.net Wed Jan 21 22:00:00 1998 Path: biosci!clo.fgov.be!j.deriek From: j.deriek@clo.fgov.be (Jan De Riek) Newsgroups: bionet.molbio.rapd Subject: SAMPL and SSR-primers Date: 22 Jan 1998 02:02:30 -0800 Organization: DvP Lines: 19 Sender: daemon@net.bio.net Distribution: world Message-ID: <34C71919.8FA@clo.fgov.be> Reply-To: j.deriek@clo.fgov.be NNTP-Posting-Host: net.bio.net Dear newsgroupers, I want to use 5'anchored SSR-primers in a modified AFLP-protocol called SAMPL. SAMPL = Selective Amplification of Microsatellite Polymorphic Loci, it uses the adaptor ligation stock from the AFLP-protocol. Then a PCR-amplification is done by using the AFLP touchdown PCR-profile with the Eco RI-primer and the labelled SSR-primer. I've searched already a lot but i've found only a few articles and abstracts with very little information ! Does anyone have experience with this technique ? Thanks a lot in advance. Jochen Dendauw E-mail : j.deriek@clo.fgov.be From owner-rapd@net.bio.net Tue Jan 27 22:00:00 1998 Path: biosci!agate!logbridge.uoregon.edu!news-peer.gip.net!news.gsl.net!gip.net!howland.erols.net!newsxfer.itd.umich.edu!uunet!in2.uu.net!comp.vuw.ac.nz!news.hn.netlink.co.nz!fri.cri.nz!NewsWatcher!user From: yoonm@fri.cri.nz (Minsoo Yoon) Newsgroups: bionet.molbio.rapd Subject: Re: SAMPL and SSR-primers Date: Wed, 28 Jan 1998 15:18:13 +1200 Organization: New Zealand Forest Research Institute Lines: 48 Distribution: world Message-ID: References: <34C71919.8FA@clo.fgov.be> NNTP-Posting-Host: 163.7.5.167 It seems to me that one should decide first whether he/she wants single locus or multi-locus profile and also whether he/she is willing to pay enough expenses to develop single-locus markers. AFLP and RAPD are basically multi-locus polymorphisms. The information contents of such procedures may be increased with the co-use of SSR-primers, for example. However, they are still multi-locus procedures, which won't correct the basic problem of multi-locus profiles at all. One have to remember that the problem associated with multi-locus procedure is not the information contents but the lack of reproducibility, specificity, transportability and also their anonymous nature. That's why they can be fairly useful for fingerprinting but not for mapping. Besides, for the SSR to be useful, it should be single-locus. In other words, it should be amplified with two specific primers. No matter how effective the anchores may be (regardless of whether 5' of 3' direction), slippage will occur to a varying degree, which will result in stuttering patterns. In article <34C71919.8FA@clo.fgov.be>, j.deriek@clo.fgov.be wrote: > Dear newsgroupers, > > I want to use 5'anchored SSR-primers in a modified AFLP-protocol called > SAMPL. > SAMPL = Selective Amplification of Microsatellite Polymorphic Loci, it > uses the adaptor ligation stock from the AFLP-protocol. Then a > PCR-amplification is done by using the AFLP touchdown PCR-profile with > the Eco RI-primer and the labelled SSR-primer. > > I've searched already a lot but i've found only a few articles and > abstracts with very little information ! > Does anyone have experience with this technique ? > > > Thanks a lot in advance. > > Jochen Dendauw > > E-mail : j.deriek@clo.fgov.be