From owner-rapd@net.bio.net Tue Feb 03 22:00:00 1998
Path: biosci!TANGELO.LAL.UFL.EDU!cxchen
From: cxchen@TANGELO.LAL.UFL.EDU ("Chunxian Chen")
Newsgroups: bionet.molbio.rapd
Subject: subscribe
Date: 4 Feb 1998 08:24:42 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 27
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <01bd3186$18bc1540$4887b096@gmitter_lab1.lal.ufl.edu>
NNTP-Posting-Host: net.bio.net
This is a multi-part message in MIME format.
------=_NextPart_000_0004_01BD315C.2FE60D40
Content-Type: text/plain;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
------=_NextPart_000_0004_01BD315C.2FE60D40
Content-Type: text/html;
charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
------=_NextPart_000_0004_01BD315C.2FE60D40--
From owner-rapd@net.bio.net Thu Feb 12 22:00:00 1998
Path: biosci!ETMP.COM!etmp
From: etmp@ETMP.COM
Newsgroups: bionet.molbio.rapd
Subject: Eventemp Stock(ETMP) Heating Up - Huge Orders Coming In!!
Date: 13 Feb 1998 13:03:35 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 41
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199802132100.QAA12760@apollo.redcomet.net>
NNTP-Posting-Host: net.bio.net
You have been carefully selected to receive the following as a
person obviously interested in this subject based upon your
previous internet postings, or visits to one of our affiliate web
sites. If you have received this message in error, please accept
our apology as a responsible e-mailer, and reply with the word
REMOVE in the subject line. You will be automatically excluded
from future e-mailings. Thank you for your consideration and help
in making the Internet spam-free. *****
*** News Flash *** 01/30/98 - Eventemp receives 27 million dollar contract - Company
expects net profit of 5 to 6 million dollars. Other large contracts pending. Huge profit
potential.
Analyst say look for Eventemp (Symbol OTC BB: ETMP) to go as high as $10.00
by March 1. Analysts predict that stock could shoot to $6.00 in the next few days.
Stock rose over 41% on 01/30/98 - Get in while there is still time.
For complete info, visit http://www.etmp.com
Now you can call your car from any available telephone and start
the air conditioner or heater and have it pre-cooled or pre-heated for
your comfort when you arrive....WITH THE VEHICLE'S ENGINE STILL
OFF!
THE TECHNOLOGY EXISTS!
THE SYSTEM WORKS!
THE COMPANY IS EVENTEMP!
THE STOCK (OTC BB: ETMP) currently trades at about $4.25 and could
possibly reach $10.00 by March 1, 1998!
EVENTEMP owns the exclusive patent rights to this revolutionary system
which will be available as an option on luxury cars this year. Deals are
in the works to make this available on all new cars by the year 2000!
Look for some exciting news to be released soon!
Visit EVENTEMP'S web site at http://www.etmp.com for more
information!
The temperature in your car may be cool, but this stock is going to be one
of the HOTTEST of 1998!
From owner-rapd@net.bio.net Mon Feb 16 22:00:00 1998
Path: biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.molbio.rapd
Subject: BIOSCI/bionet miniFAQ & Fundraiser
Date: 17 Feb 1998 02:00:05 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 233
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199802171000.CAA11767@net.bio.net>
NNTP-Posting-Host: net.bio.net
(LAST REVISION: 30-JUL-95)
This BIOSCI "miniFAQ" is designed to answer the questions that come up
the *most frequently*. The main BIOSCI FAQ (Frequently Asked
Questions) is accessible on the World Wide Web at URL
http://www.bio.net/.
If you can not find an answer to your question in this or other
documentation, the BIOSCI technical support staff answers e-mail
queries sent to
biosci-help@net.bio.net
We can only answer questions about the use of the newsgroups and
mailing lists. We unfortunately do not have the staff to do Internet
information searches or answer scientific questions. Please post
those to the appropriate BIOSCI/bionet newsgroups.
Contents:
--------
0) BIOSCI NEEDS YOUR SUPPORT!!
1) Using the WWW to access the BIOSCI/bionet newsgroups.
2) What to do about "spams," i.e., junk mail, ads, etc.
3) Examples of subscribing and unsubscribing to the mailing lists.
4) The BIOSCI user address and research interest directory.
0) BIOSCI NEEDS YOUR SUPPORT!!
------------------------------
BIOSCI's government funding has been expended, and we are now
operating solely from advertising revenue that we have raised from our
Web site at http://www.bio.net/. We need just a few minutes of your
time to help us serve you.
You can do two important things which will take very little time for
you individually and will immensely help us continue to help you.
First, please use our WWW system at http://www.bio.net/ to access the
archives. You can post or reply to messages via your Web browser as
described in item #1 below. Your usage helps attract sponsors. If you
contact any of our sponsors, please be sure to thank them for
supporting BIOSCI. It is critical for them to get this feedback if
they are to continue their sponsorship for the long term.
Second, if you work for a company or organization that provides
products or services of interest to the biology community, please pass
this message on to your marketing or marketing communications
department or other appropriate group. Please ask them to help
support BIOSCI by sponsoring our Web site and explain the uses and
benefits of the system to the biology community. If they are
interested, they can then contact us for further information at our
tech support address, biosci-help@net.bio.net.
1) Using the WWW to access the BIOSCI/bionet newsgroups.
--------------------------------------------------------
As of 10 December 1995, all BIOSCI/bionet full newsgroups are
accessible through the World Wide Web (WWW) at URL http://www.bio.net.
One can read and reply publicly or privately to both recent postings
and archived messages through one's Web browser if it is configured
properly to send e-mail. Each newsgroup is equipped with its own WAIS
index. The main BIOSCI home page also has access to the BIO-JOURNALS
Table of Contents database WAIS index and the BIOSCI user address
database described in another item further below.
2) What to do about "spams," i.e., junk mail, ads, etc.
-------------------------------------------------------
BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups),
mailing lists, and a hypermail archive at URL http://www.bio.net/.
The same postings are distributed on all media (except for a small
number of mailing-list-only groups at net.bio.net). Unfortunately it
is becoming a despicable practice on the Internet (by a few people out
to make a fast buck) to do automated mass postings to thousands of
newsgroups and mailing lists. These attempts to grab free advertising
are refered to as "spams" in the usual, somewhat boneheaded, net
terminology. USENET is more susceptible to this practice, and many
spams originate on the USENET groups and then are passed on to the
mailing lists. However, spammers also get lists of mailing addresses
and hit these too, so neither medium is immune.
What should you do personally if you get junk mail?
---------------------------------------------------
Just delete it and move on without reading it further. Filing a
protest is becoming increasingly useless because spammers are often
disguising the addresses where the messages are sent from. Unless you
really understand Internet mail systems, your attempt at protest by
sending replies to the message will often end up being sent to the
address of an innocent person that the spammer is victimizing.
What can BIOSCI/bionet do to protect its newsgroups?
----------------------------------------------------
The only solution currently available is to moderate the newsgroup.
If this newsgroup is already moderated, then you are in good shape.
Moderation protects the USENET distribution from about 95% of the
spams that are being sent to date and protects the mailing lists
completely. Moderation means, however, that someone has to take the
time to review each message before it goes out. We have set up
software here that simply allows the moderator to forward to an
address at net.bio.net messages that (s)he wishes to have distributed.
This takes no more time than that needed to read the message and pass
it on, say about 1 min. per message.
Most newsgroups currently have a discussion leader who is responsible
for their newsgroup. The discussions leaders and their e-mail
addresses are listed in the BIOSCI Information Sheet which is
available on the Web at http://www.bio.net/. If a newsgroup is being
hit with too many junk postings, please contact the discussion leader
for that group and see if there is interest in moderating the group.
Please do not assume that by simply posting a complaint to the
newsgroup itself, anyone on the BIOSCI staff will act on your
complaint. With close to 100 newsgroups to run, the BIOSCI staff has
to rely on the discussion leaders of each newsgroup to report problems
directly to us at biosci-help@net.bio.net.
We will moderate any of our newsgroups if the discussion leader tells
us that the readership of the group wishes to do so and if a moderator
is willing to do the work. For most BIOSCI/bionet groups, this
entails only a few minutes of work each day.
Moderating a newsgroup will resolve probably 95% of the junk postings
on the USENET distribution. Unfortunately there are easy ways for
determined spammers to override the moderation mechanism on USENET,
but we can protect our e-mail subscribers from unwanted postings if
the newsgroup is moderated. You can also access our newsgroups over
the WWW at URL http://www.bio.net. While this Web interface will not
stop spammers from trying to post to the groups, this will give you
yet another way, besides using USENET news, to keep the junk out of
your personal mail files. For those of you with local USENET news
systems, the Web interface will also give you faster access to new
newsgroups and recent postings.
3) Examples of subscribing and unsubscribing to the mailing lists.
------------------------------------------------------------------
PLEASE NOTE: The BIOSCI management does NOT act on
subscription/unsubscription requests that are posted improperly to the
newsgroups and mailing lists. People who do this only bother everyone
on the lists to no avail. Please be sure to follow the proper
procedures below.
Gory details are in the BIOSCI Information sheets on the Web at
http://www.bio.net. Below we give an example utilizing the
METHODS-AND-REAGENTS list at both of our two BIOSCI sites:
Users in the Americas and Pacific Rim countries who use the BIOSCI
------------------------------------------------------------------
node at computer net.bio.net:
----------------------------
A) Determine the "listname" which is the <=8 character mail address
^^^^^^^^^^^^^
for the group. These can be found in the BIOSCI Info. Sheet. For
the METHODS-AND-REAGENTS group the mailing address is
methods@net.bio.net. The listname is the portion of the address to
the left of the @ sign, i.e., "methods". The listname is used with
the "subscribe" and "unsubscribe" commands illustrated below.
B) Mail all commands in the body of a mail message addressed to
biosci-server@net.bio.net. Do NOT send commands to the newsgroup
posting addresses! Leave the Subject: line blank, any text on it
will be ignored.
C) In the body of your message put one or more of the following
commands with an "end" command on the last line, e.g.,
subscribe methods
unsubscribe methods
end
Do NOT put your e-mail address or other text on these lines. The
server only allows you to cancel your subscription if the address
on your mail header matches the address on our mailing list.
Please ask for help at biosci-help@net.bio.net if your address has
changed, e.g., if you know you are on the list but the server tells
you that you are not a member.
Users in Europe, Africa, and Central Asia who use the BIOSCI node at
--------------------------------------------------------------------
computer daresbury.ac.uk (also known as dl.ac.uk):
-------------------------------------------------
To subscribe and unsubscribe to/from the BIOSCI lists, you need to
specify the full USENET newsgroup name with "bionet-news." prepended.
The USENET newsgroup names are listed in the BIOSCI Information sheet
on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list
the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the
appropriate commands are
sub bionet-news.bionet.molbio.methds-reagnts
unsub bionet-news.bionet.molbio.methds-reagnts
These commands are included in a message addressed to mxt@dl.ac.uk,
NOT to the newsgroup mailing addresses. As usual, include the text in
the body of the message as text on the Subject: line is ignored.
To unsubscribe from all the lists at the UK node, use
unsub bionet-news
Please note that if the address in the list is different than the one
in your mail message header, you will not be able to unsubscribe by
this method. If you have problems, please mail biosci@daresbury.ac.uk.
4) The BIOSCI user address and research interest directory.
-----------------------------------------------------------
Please take this opportunity to add your name, address, and research
interest information to the BIOSCI User Address Database if you have
not already done so.
You can fill out the address form directly through our Web page at URL
http://www.bio.net/adrform.html.
The address database is reindexed nightly for WWW access (the URL is
http://www.bio.net/). If you are not directly on the Internet but can
reach it by e-mail, please use our waismail server to access the user
directory. waismail use is described above. You can also request a
user address form by e-mail from biosci-help@net.bio.net.
Please check your database entry from time-to-time to see if your
address information is still up-to-date. Because of our limited
personnel resources, we ask that you resubmit a *complete* form to
revise your entry; we only replace complete entries and do not have
resources to edit old forms.
From owner-rapd@net.bio.net Mon Feb 16 22:00:00 1998
Path: biosci!embratel.net.br!fleisch
From: fleisch@embratel.net.br
Newsgroups: bionet.molbio.rapd
Subject: yeast stability
Date: 17 Feb 1998 10:56:49 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 8
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199802171855.QAA13704@rjo04.embratel.net.br>
NNTP-Posting-Host: net.bio.net
Hi.
Could anybody suggest what I should add to the aerobic fermentation medium in
order to increase yeast(Saccharomyces cerevisiae) stability? Vitamins or salts?
I would appreciate very much.
Thank you
From owner-rapd@net.bio.net Tue Feb 24 22:00:00 1998
Path: biosci!TUSK.EDU!Prakash
From: Prakash@TUSK.EDU ("C. S. Prakash")
Newsgroups: bionet.molbio.rapd
Subject: International Horticultural Congress in Belgium (August 1998)
Date: 25 Feb 1998 11:39:24 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 55
Sender: daemon@net.bio.net
Distribution: world
Message-ID:
NNTP-Posting-Host: net.bio.net
Detailed information on the International Horticultural Congress in
Brussels, Belgium (August 2-7, 1998) can be found on the Internet at
http://www.agr.kuleuven.ac.be/ishs/IHC/third.htm
Email for additional Information: 25ihc98@tornado.be
Fax: 32 2 206 7209
Unfortunately, the deadline for abstracts is over but as you can see from
selected programs I have pasted below, there are many sessions/workshops
related to plant molecular biology, tissue culture and biotech, and thus
worth considering. There will be few travel awards for scientists
presenting papers from developing countries. Please contact the organizers
directly!
Prakash
=================
Friday, 7 August 1998
Theme 4: Application of biotechnology and molecular biology
Conveners: L.H.W. van der Plas (NL) and M. Welander (Sweden)
Introductory lecture: F. Salamini, Cologne, Germany
Subtheme 4.1: In-vitro culture (hormonal effects, somatic embryogenesis,
variation etc.) Conveners: G.J.M. de Klerk (NL), R.A. Drew (Australia)
Subtheme 4.2: Genetic manipulation and breeding (techniques, stability,
cell competence etc.) Conveners: J.J.M.Dons (NL), D. Cohen (New Zealand)
Subtheme 4.3: Gene isolation and expression (disease resistance, quality,
abiotic stress etc.) Conveners: I.J. Vanderleyden (B), W.R. Woodson (USA)
Subtheme 4.4: Genome analysis (indirect selection, marker techniques,
finger-printing etc.) Conveners: E. Van Bockstaele (B), M. Delseny (France)
Workshops
EW 2: Genetic engineering; important horticultural traits (Tuesday, 4 August)
Conveners: R. Zimmerman (USA), C. Damiano (Italy), P. Debergh*
EW 4: Biotechnology below ground - enhancing molecular biology research of
root crops (Thursday, 6 August) Convener: C.S. Prakash* (USA)
****************** ****************** ****************** *******
C. S. Prakash Prakash@tusk.edu
Center for Plant Biotechnology Research Ph: (334) 727 8023
College of Agricultural, Env and Nat Sci. Fax:(334) 727 8067
Tuskegee University http://agriculture.tusk.edu/
Tuskegee, AL 36088, USA
****************** ****************** ****************** *******
From owner-rapd@net.bio.net Tue Feb 24 22:00:00 1998
Path: biosci!SCOTUS.SFCPA.EDU!JJTFA1
From: JJTFA1@SCOTUS.SFCPA.EDU ("John Trimble")
Newsgroups: bionet.molbio.rapd
Subject: molluscan study
Date: 25 Feb 1998 11:57:34 -0800
Organization: Saint Francis College
Lines: 14
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <4AE67836381@scotus.sfcpa.edu>
NNTP-Posting-Host: net.bio.net
We have an undergraduate research project planned to study the
polymorphisms of the freshwater limpet, _Ferrissia rivularis_ using
RAPD. I have not yet found any reports of RAPD used on molluscs.
I wonder if RAPD with molluscs will present any special problems?
Any advice or comment welcome.
Thanks.
John J. Trimble
Biology Dept.
St. Francis College
Loretto, PA
(814) 472-3319
jtrimble@sfcpa.edu
From owner-rapd@net.bio.net Thu Feb 26 22:00:00 1998
Path: biosci!agate!logbridge.uoregon.edu!news.maxwell.syr.edu!newsfeed.internetmci.com!152.163.199.19!portc03.blue.aol.com!audrey01.news.aol.com!not-for-mail
From: haroldb786@aol.com (HaroldB786)
Newsgroups: bionet.molbio.rapd
Subject: Re: molluscan study
Date: 27 Feb 1998 03:24:36 GMT
Lines: 10
Message-ID: <19980227032400.WAA10526@ladder02.news.aol.com>
NNTP-Posting-Host: ladder02.news.aol.com
X-Admin: news@aol.com
Organization: AOL http://www.aol.com
References: <4AE67836381@scotus.sfcpa.edu>
My only experience with RAPD's was a two month frustration packed adventure
with dungenese crabs.... RAPD's are very touchy with DNA concentration.... I
was working with an antique Coy...... Of course, you will start off with a
bank of 20 primers and try them on a representative-geographic sample of
limpets..... As long as you can extract quality DNA you are more than half way
there...
have fun!!!!
harold berninghausen
From owner-rapd@net.bio.net Thu Feb 26 22:00:00 1998
Path: biosci!agate!logbridge.uoregon.edu!newsfeed.direct.ca!news.UVic.CA!usenet
From: Bug Hunter
Newsgroups: bionet.molbio.rapd
Subject: Assessing genetic variation using RAPD's
Date: Fri, 27 Feb 1998 08:10:39 -0800
Organization: University of Victoria
Lines: 9
Message-ID: <34F6E57F.239B@uvic.ca>
NNTP-Posting-Host: bugkiller.ceh.uvic.ca
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.0Gold (Win95; I)
Hi,
Does anyone know of a statistical test that will quantify the
genetic variation observed from RAPD bands in a population, if each
individual has a different banding pattern?
-Tom Clarke-
Dept. of Biology
University of Victoria
From owner-rapd@net.bio.net Fri Feb 27 22:00:00 1998
Path: biosci!agate!howland.erols.net!surfnet.nl!news-ge.switch.ch!news-zh.switch.ch!elna.ethz.ch!not-for-mail
From: Roland Koelliker
Newsgroups: bionet.molbio.rapd
Subject: Re: Assessing genetic variation using RAPD's
Date: Sat, 28 Feb 1998 12:57:24 +0100
Organization: Swiss Federal Institute of Technology (ETHZ)
Lines: 17
Message-ID: <34F7FBA4.2D00@ipw.agrl.nospam.ethz.ch>
References: <34F6E57F.239B@uvic.ca>
Reply-To: roland.koelliker@ipw.agrl.nospam.ethz.ch
NNTP-Posting-Host: ipw-cp-225.ethz.ch
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.04Gold (Win95; I)
Hi Tom
I don't exactly understand what you want to test, but you might try
analysis of molecular variance (AMOVA) which allows to partition the
different sources of variation and offers a test (Bartlett's
hetereoscedascity index) to compare within source variation. AMOVA is
available under
http://anthropologie.unige.ch/~laurent/default.htm#Software programs.
Hope this helps
Roland
--
**********************************************
To reply remove nospam from address
***********************************************