*********************************************************************

Valeria Maida

&nbs= p;

Tel. + 39 79 229542/229588

Dipartimento di Chimica fax + 39 79 229559/229482

via Vienna, 2

e-mail billia@ssmain.uniss.it

07100 Sassari

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********************************************************************* From owner-rapd@net.bio.net Thu Apr 16 23:00:00 1998 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Apr 1998 02:00:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199804170900.CAA27086@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-rapd@net.bio.net Sat Apr 18 23:00:00 1998 Path: biosci!CGNET.COM!A.THRO From: A.THRO@CGNET.COM ("Thro, Ann Marie") Newsgroups: bionet.molbio.rapd Subject: What biotech tools for farmers research Date: 18 Apr 1998 20:15:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 97 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Colleagues: The CGIAR System -Wide Program on Participatory Research and Gender Analysis is developing a paper to examine the questions below, among others, regarding the potential role of biotech tools and processes in farmer participatory genetic improvement research., across all crop types. The objective is to establish a base-line survey of current views, stimulate debate, and identify areas for investigation. The paper will refer specifically to plant biotech (transgenesis, molecular genetics, micropropagation). The target farmers are small holder farmers or sharecroppers in the tropics, but examples from any farmer group could be revealing. Can any of you comment on any aspect of these questions? It is something of a thought experiment at this point, so science fiction is acceptable. Detailed reference to specific technology possibilities would be especially valuable. We are interest in pro and con positions, current potential and futuristic ideas, visions and cautions. Also in any citations or leads to published work. Any comments will be acknowledged and a prepublication draft sent for checking. Thank you for any comments, Ann Marie Thro, CIAT/ Cassava Biotech Network Coordinator, a.thro@cgnet.com 17 April 1998 ********************** Are there examples (real or hypothetical) of the use of biotechnology in participatory plant breeding projects which involve both researchers and farmers? Relative to traditional breeding, are there advantages (or disadvantages) for farmers of breeding products or processes based on biotechnology innovations? If so, what are they? Can biotechnology accommodate the needs of different groups in a community (e.g. male- vs. female- headed households, large farmers, sharecroppers vs. smallholders, others) and different members of the household? Can biotechnologies help to overcome constraints in farmers' access to inputs, knowledge and other resources? Does use of biotechnology require greater emphasis on identifying generic vs. situation-specific needs? (for reasons technical? financial?logistic?) What might biotechnology-assisted participatory plant breeding methods be comprised of? How similar or differ from participatory plant breeding based upon traditional plant breeding? Would farmers' (or researchers) participation in a breeding project, be affected by use of biotech tools (would it differ from participation in a project not using biotechnology tools)? How? And vice versa, would farmer participation affect the way biotechnology tools are used? Can more efficient division of labour/skills/resources be devised for greater research impact? What are the cost-benefit implications? What information exist as to how benefits of farmer participation may balance costs? Do (or could) some biotechnologies have potential for improving farmers own breeding products or processes? Can farmers use some biotechnologies to enhance their own breeding efforts? If so, what types of biotechnologies may be of most direct utility to farmers? If not, could such biotechnologies be developed? Can biotechnologies applied to their own breeding processes and products help farmers overcome limited access to inputs and resources (including knowledge )? Can biotechnology develop resources which can be renewed by farmers, without outside assistance? (real or hypothetical examples) or help open new horizons to farmers through their own breeding efforts? Would biotechnology introduce new simplicities or complexities in farmers' breeding efforts, and if so how might these affect and be dealt with by farmers? How can or would farmers learn about and access useful biotechnology methods or tools? When multiple research approaches (biotechnology among them) could be used to meet a farmer need or opportunity, what criteria might be used to decide? Who decides? What factors do/should influence the decision to target biotechnology research at a specific local environment or at a national or regional level? To what extent can or do farmers (or farmers groups) participate in each such decision making? Are there examples, successful or otherwise? What biosafety issues are specific to farmer-participatory research? Who decides risk from GMOs and on what basis? Who decides what risk farmers should be exposed to? Do /can farmers' groups participate in biotech risk assessment procedures? How? Are farmers biosafety-risk averse or not? Will risk assessment procedures limit farmer access to biotechnologies, especially on a participatory experimental level? Do farmers have a right to experiment with transgenes? Can biotechnology assisted participatory plant breeding be done with existing biosafety legislation models? Examples? ************ From owner-rapd@net.bio.net Mon Apr 20 23:00:00 1998 Path: biosci!CLEMSON.EDU!rwing From: rwing@CLEMSON.EDU (Rod Wing, by way of C. S. Prakash) Newsgroups: bionet.molbio.rapd Subject: Genomics Institute Positions Date: 20 Apr 1998 20:24:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 60 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Folks, Please post this Ad. Thanks, Rod Wing CLEMSON UNIVERSITY GENOMICS INSTITUTE - POSITIONS An international center focusing on structural and functional genomics of agriculturally important crops and their pests (http://genome.clemson.edu). Two Research Associate/Assistant Professor and 10 technician positions are available immediately. Research Associate/ Assistant Professor - Physical Mapping. Scientist will oversee a physical mapping team of 8 technicians to develop sequence ready BAC physical maps. Experience with large insert DNA libraries and IMAGE and FPC Unix software strongly preferred. Research Associate/ Assistant Professor - DNA Sequencing. Scientist will oversee a team of 8 technicians to perform high-through-put DNA sequencing projects on BAC ends and whole BACs using 9 ABD377 DNA sequencers. Experience running a high-through-put DNA sequencing lab is strongly preferred. Technicians will work in one of four areas of the Institute: BAC library construction and analysis, Physical mapping, DNA sequencing and Bioinformatics. Clemson University is a public land grant institution located in the foothills of the Smoky Mountains. Highly motivated individuals should apply by email to Drs. Rod Wing (rwing@clemson.edu) and Ralph Dean (rdean@clemson.edu). Clemson University Genomics Institute, 100 Jordan Hall, Clemson University, Clemson, SC 29634. Applicants should include a curriculum vitae, a brief description of research interests and names and email addresses of 3 references. Deadline for receipt of application is May 15, 1998. From owner-rapd@net.bio.net Mon Apr 20 23:00:00 1998 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newshub.northeast.verio.net!hammer.uoregon.edu!leto.ou.edu!news.ecn.uoknor.edu!news.okstate.edu!nntp.ksu.edu!news.physics.uiowa.edu!newsrelay.iastate.edu!news.iastate.edu!not-for-mail From: Michael Baker Newsgroups: bionet.molbio.rapd Subject: microsat enrichment Date: Mon, 20 Apr 1998 11:27:05 -0500 Organization: Iowa State University Lines: 29 Message-ID: <353B7758.54A6098D@iastate.edu> NNTP-Posting-Host: ekgrads.ent.iastate.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii; x-mac-type="54455854"; x-mac-creator="4D4F5353" Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.04 (Macintosh; I; PPC) Dear all, I am interested in using the method described by Edwards et al. 1996 (Biotechniques 20:758-760) for microsatellite enrichment in the Tsetse fly, Glossina morsitans. I am fairly new to the world of microsatellites and would therefore like to hear about the experiences of others using this or similar methods of enrichment. I am particularly interested in hearing about any problems others have encountered. Also I would like to hear about any other adaptor/plasmid systems others are using. I was thinking about using the adaptors described in Edwards et al. 1996 substituting a BglII site for the MluI site and then cloning into the BamHI site of pGem-7. Any information will be appreciated. Thanks, Mike -- __________________________ Michael D. Baker, Ph.D. Department of Entomology 411 Science II Building Iowa State University Ames, IA 50011 email: mdbaker@iastate.edu phone: (515) 294-1815 fax: (515) 294-5957 From owner-rapd@net.bio.net Fri Apr 24 23:00:00 1998 From: ADULTS ONLY!!!!!!!!!! Newsgroups: bionet.molbio.rapd Date: Sat, 18 Apr 1998 22:16:02 PDT Subject: 4" CLITS LICKED BY HOT TEENS AND CELEBS! Organization: Email Platinum v.3.1b NNTP-Posting-Host: 209.136.121.149 Message-ID: <353978e8.1@news.codenet.net> Lines: 19 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!199.165.217.102!news.corpcomm.net!news.gate.net!news1.acsi.net!news.codenet.net!209.136.121.149 3 OF THE BEST NO BULLSHIT SITES AROUND!!!!!!!!!!!!1 JUST CLICK HERE ONCE AND SEE 4 YOURSELF!!!!!!! http://pics.xxxhosting.net http://pics.xxxhosting.net/teens.html -101 new school age teens every day!! http://pics.xxxhosting.net/celebs.html 69 new celeb pics daily http://pics.xxxhosting.net/asian.html 1000 asian pics daily no bull shit here just free xxx picss!! From owner-rapd@net.bio.net Fri Apr 24 23:00:00 1998 Path: biosci!news.Stanford.EDU!Cabal.CESspool!bofh.vszbr.cz!news.maxwell.syr.edu!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: mastewar@badlands.nodak.edu Newsgroups: bionet.molbio.rapd Subject: graduate student position Date: Sat, 25 Apr 1998 09:57:45 -0600 Organization: Deja News - The Leader in Internet Discussion Lines: 47 Message-ID: <6hstla$vkq$1@nnrp1.dejanews.com> NNTP-Posting-Host: 134.129.113.2 X-Article-Creation-Date: Sat Apr 25 14:57:45 1998 GMT X-Http-User-Agent: Mozilla/3.01 (Win95; I) Graduate Student Position Opening in Cell, Molecular and Developmental Biology for fall, 1998 or January, 1999. A funded graduate student position, including full tuition waiver, is open in my laboratory beginning in fall, 1998 or January 1999. We offer graduate programs leading to the Ph.D. in Zoology http://www.ndsu.nodak.edu/zoology/ or in Cellular and Molecular Biology http://www.ndsu.nodak.edu/instruct/mcclean/ag_www/grad_cmb.htm We also have a Masters degree program in Zoology. Current projects in my laboratory involve investigation of the Drosophila p70 S6 kinase in oogenesis, development and investigation of the signal transduction pathway leading to p70 S6 kinase activation (see Stewart et al., 1996, PNAS, 93:10791-10796). (Also see http://www.ndsu.nodak.edu/ndsu/mastewar/). The mammalian p70 S6 kinase is thought to be involved in regulating the translation of a specific class of mRNAs encoding products required for cell growth and translation. Consistent with a role for this kinase in regulating translation, mutations in the Drosophila p70 S6 kinase disrupt oogenesis and cause a Minute-like phenotype, representing the first reported non-ribosomal protein Minute. We are using clonal analysis of hypomorphic and null alleles to determine the tissue requirements of this kinase. A second project involves genetic screens for components of the S6 kinase signaling pathway. If you know of a student interested in Drosophila genetics and development, cell biology and cell signaling, and considering graduate school, please pass this announcement on to them. Information concerning how to contact me is below. Mary Stewart Dept. of Zoology Stevens Hall North Dakota State Univ. Fargo, ND 58105 United States phone: (701) 231-8226 fax: (701) 231-7149 email: mastewar@badlands.nodak.edu -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/ Now offering spam-free web-based newsreading From owner-rapd@net.bio.net Wed Apr 29 23:00:00 1998 Path: biosci!PHARMAJOBS.COM!vcornford From: vcornford@PHARMAJOBS.COM Newsgroups: bionet.molbio.rapd Subject: International Pharmajobs Date: 30 Apr 1998 05:46:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <199804301244.OAA11146@mail.nacamar.de> NNTP-Posting-Host: net.bio.net Yet another opportunity provided by International Pharmajobs! Since 1996 International Pharmajobs has been providing the Pharmaceutical Industry with an up to the minute Job Market in the Internet. Companies such as Merck, Hoechst Marion Roussel, Bayer and BASF Pharma use these pages to publish their job opportunities, everything from Trainee positions through to top management postings. We have now opened a new service to make life easier for anyone seeking a job on our pages, a service that keeps you informed without requiring you to stay constantly on-line. Every time that a company posts a new position on our site our members will be automatically notified of it by e-mail. This saves time and ensures that the member is always up to date with the latest offers. If you are interested in knowing more about this service then read about it at http://www.pharmajobs.com/spring/special2.htm We believe that today it is particularly important to offer support and help to job-seekers in order to make a difficult task as manageable as possible. Yet another opportunity provided by International Pharmajobs! For further information contact: Vivian Cornford R.S.V.P Personalvermittlung Reuterweg 51 - 53 60323 Frankfurt am Main Tel: +49 69 969666-15 Fax. +49 69 959666-11 e-mail: vcornford@pharmajobs.com