From owner-rapd@net.bio.net Sun Aug 02 23:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!howland.erols.net!sunqbc.risq.qc.ca!news.uow.edu.au!news.usyd.edu.au!not-for-mail From: Stephen Graham Newsgroups: bionet.molbio.rapd Subject: Genetic evolution of primates Date: Mon, 03 Aug 1998 21:15:57 +1000 Organization: The University of Sydney, Australia Lines: 7 Message-ID: <35C59BED.EAD5839@mail.usyd.edu.au> NNTP-Posting-Host: mp-10-33.mp.usyd.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: metro.ucc.usyd.edu.au 902142955 12060 129.78.57.219 (3 Aug 1998 11:15:55 GMT) X-Complaints-To: usenet@news.usyd.edu.au NNTP-Posting-Date: 3 Aug 1998 11:15:55 GMT X-Mailer: Mozilla 4.03 [en] (Win95; I) If anyone is knowledgeable as to similarities in primate/human DNA, has any statistics on this or knows of any good links could they please drop me a line. Thanks Stephen From owner-rapd@net.bio.net Mon Aug 03 23:00:00 1998 Path: biosci!daresbury!not-for-mail From: deepak@bgumail.bgu.ac.il (Deepak Khandka) Newsgroups: bionet.molbio.rapd Subject: Are there reports of identifying RAPD probe for functional gene ? Date: 4 Aug 1998 10:01:32 +0100 Lines: 16 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <6q6ilc$mc@mserv1.dl.ac.uk> Original-To: rapd@dl.ac.uk Hi there, I would like to know if there are more reports of identifying RAPD or SCAR marker that represented (as a probe to) a functional gene. I found following reference: Garcia D.K., DharA.K. and Alcivar-Warren A. Molecular analysis of a RAPD marker (B20) reveals two microsatellites and differential mRNA expression in Penaeus vannamei. Molecular Marine Biology and Biotechnology 5(1): 71-83(1996) Deepak Khandka Campus Sede Boker Ben-Gurion University Israel From owner-rapd@net.bio.net Tue Aug 04 23:00:00 1998 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!news.maxwell.syr.edu!news-peer.gip.net!news.gsl.net!gip.net!portc01.blue.aol.com!newsstand.cit.cornell.edu!syntom.plbr.cornell.edu!user From: alm13@cornell.edu (Andreas Matern) Newsgroups: bionet.molbio.rapd Subject: Re: Genetic evolution of primates Date: Wed, 05 Aug 1998 18:26:44 -0400 Organization: Cornell University Lines: 28 Sender: alm13@cornell.edu (Verified) Message-ID: References: <35C59BED.EAD5839@mail.usyd.edu.au> NNTP-Posting-Host: syntom.plbr.cornell.edu Here's some info from PubMed: http://www.ncbi.nlm.nih.gov/cgi-bin/Entrez/referer?/htbin-post/Entrez/query%3fdb=m&form=6&uid=9585431&Dopt=m Hope it helps...... In article <35C59BED.EAD5839@mail.usyd.edu.au>, Stephen Graham wrote: > If anyone is knowledgeable as to similarities in primate/human DNA, has > any statistics on this or knows of any good links could they please drop > me a line. > > Thanks > Stephen -- Andreas Matern alm13@cornell.edu 266 Emerson Hall Dept of Plant Breeding and Biometry Cornell University Ithaca, NY 14853 http://www.people.cornell.edu/pages/alm13/ From owner-rapd@net.bio.net Tue Aug 11 23:00:00 1998 Path: biosci!TUSK.EDU!prakash From: prakash@TUSK.EDU ("C. S. Prakash") Newsgroups: bionet.molbio.rapd Subject: Monsanto Funding for Arabidopsis DNA Chip Research Date: 11 Aug 1998 17:57:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 69 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Monsanto is funding research on Arabidopsis EST Microarray-DNA Chip Plant Biology Research and providing access to this 'hot' technology for genome researchers. For more details visit the Monsanto web site (http://www.monsanto.com/arabidopsis) or send a request by mail to Arabidopsis Academic Program, Monsanto Company, Mail Stop O3A, 800 North Lindbergh Boulevard, St. Louis, Missouri 63167, or by fax to Arabidopsis Academic Program, Monsanto Company, (314) 694-2303. For information on DNA Chips, refer to my article in the September 1997 issue of ISB News Report at http://www.nbiap.vt.edu/ . I reproduce a part of the Monsanto Grant information below here, Prakash Prakash@tusk.edu ==================== BACKGROUND Arabidopsis Functional Genomics Using Microarray Technology Genomics research has been accelerated by a finite number of key technologies. These new technologies have significantly reduced the time from gene discovery to understanding the function and roles of the gene product. The cDNA microarray technology is one such tool. Using cDNA microarrays enables a researcher to analyze expression of thousands of genes with known sequence in a single experiment. The microarray technology is a recognized high-throughput method for monitoring the expression of genes. This chip-based approach using microarrays of cDNA clones as gene-specific hybridization targets allows quantitative measurements of the differential expression of the corresponding plant genes using a two-color fluorescence labeling and detection scheme. (Schena, et al., 1996, PNAS 93:10614) Monsanto Life Sciences Company, in collaboration with Synteni, Inc. of Freemont, California, is establishing this Plant Biology Microarray project to enhance plant genomics research in academia. The program will provide researchers the opportunity to access approximately 10,000 Arabidopsis Expressed Sequence Tags on a microarray. Studies in plant genetics, plant physiology, plant development, evolutionary biology, and plant metabolic regulation are particularly likely to benefit from this technology. PROCESS An advisory committee of scientists has been selected to review proposals and award access to the cDNA microarray program. Awards will be competitive and based upon scientific merit and potential impact on gene discovery and functional analysis towards understanding plant biological processes. The successful awardees must provide probe reagents (quality assured polyadenylated or total RNA). The results and quality control data in the form of quantitative relative expression levels for all annotated expressed sequence tags and other Arabidopsis ESTs on the microarray will be returned to the investigators. Academic participation requires a willingness to allow Monsanto and Synteni to access genes and information discovered as a result of this program. The Arabidopsis Academic Program will be on-going for at least 2 years with proposals being accepted in the spring and fall. Up to 50 awards will be made during each proposal and review cycle. The expectation is that an individual investigator would be funded only one time during the 2 year cycle. Important Program Dates Proposal Deadline: September 15, 1998 / March 15, 1999 / September 17, 1999 From owner-rapd@net.bio.net Sun Aug 16 23:00:00 1998 Path: biosci!rz.uni-jena.de!b5wojo From: b5wojo@rz.uni-jena.de (Johannes Woestemeyer) Newsgroups: bionet.molbio.rapd Subject: radio-isotope course Date: 17 Aug 1998 09:07:40 -0700 Organization: FSU Jena - Microbiology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <35D8546B.6EF9@rz.uni-jena.de> NNTP-Posting-Host: net.bio.net Dear colleagues, during my preparations for a freshly designed practical course in radioisotope handling I would be interested in informations frm other institutes, experiments, text books, internet sources and similar that might help to improve teaching in this field. Any hints or advice for me? Thank you for bothering, Prof. Joh. Woestemeyer Inst. Microbiology/Microbe genetics Friedrich-Schiller University Jena Neugasse 24 D-07743 Jena e-mail: b5wojo@rz.uni-jena.de From owner-rapd@net.bio.net Wed Aug 19 23:00:00 1998 Path: biosci!BCCANCER.BC.CA!AIshkani From: AIshkani@BCCANCER.BC.CA (Adrian Ishkanian) Newsgroups: bionet.molbio.rapd Subject: unique human DNA sequence Date: 19 Aug 1998 20:08:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I have been using the RAPD technique for a number of months now, and am in need of a unique human DNA sequence which I can use for an internal control. Primers suggested have all been around 17-20mers, while RAPD uses typically 10mers. Although one can design 20mers with high AT content thereby lowering the Tm to a suitable level for the RAPD protocol, I am still skeptical as to whether or not this will work. Has anyone else found a unique human DNA sequence from which they could design useable primers for internal control purposes? Thanks, Adrian Ishkanian From owner-rapd@net.bio.net Wed Aug 19 23:00:00 1998 Path: biosci!rz.uni-jena.de!b5wojo From: b5wojo@rz.uni-jena.de (Johannes Woestemeyer) Newsgroups: bionet.molbio.rapd Subject: RAPD primers Date: 19 Aug 1998 23:16:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.3.32.19980820081259.006bb174@pop3.uni-jena.de> NNTP-Posting-Host: net.bio.net Dear Adrian, I'm not sure if I really understand your problem. Nevertheless: a short note on RAPD primers might help. It does not matter very much how long RAPD primers are. Essential for the amplification products is the annealing temperature. If a decamer gives a certain pattern, a longer n-mer with identical bases at the 3'-end will give you (essentially) the same pattern. We checked this with several primers that we used for diagnosis of rape seed pathogens. There are, however, several deviations from the above-mentioned general rule. - additional bases may add to binding strength of the primers. Therefore longer primers tend to produce more bands. - in RAPD assays there is a lot of competition between the potential binding sites. Sometimes longer primers favor amplicons that you have not seen before. Other amplicons that were weak with 10mers may be lost if the 5'extension does not add to binding strength and others have improved considerably. In this case you may loose bands due to differences in competition. But: in any case we were able to recognize the general underlying RAPD pattern independent of primer length. Good luck, joh. woestemeyer, inst microbiol./microbe genetics university jena b5wojo@rz.uni-jena.de ------------------------------------------------------- Prof. Dr. Johannes Woestemeyer Institute of General Microbiology and Microbe Genetics Friedrich-Schiller-University Jena Neugasse 24 D-07743 Jena - Germany Tel. : +49 (0)3641 949310/1 Fax : +49 (0)3641 930312 E-Mail : b5wojo@rz.uni-jena.de Homepage: http://www.uni-jena.de/biologie/mikrobio/ -------------------------------------------------------- From owner-rapd@net.bio.net Thu Aug 20 23:00:00 1998 Path: biosci!lakshya.kalptaru.com!Skatiyar From: Skatiyar@lakshya.kalptaru.com (Skatiyar) Newsgroups: bionet.molbio.rapd Subject: Subscribe Date: 20 Aug 1998 20:00:15 -0700 Organization: Kalptaru Net ! Central India. 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NEW,NEW,NEW $10 MAIL IN REBATE ON SELECTED CARTRIDGES SIMPLY ORDER YOUR NEW CARTRIDGE, WHEN IT ARRIVES MAIL BACK YOUR REBATE CERTIFICATE WITH ANY USED CARTRIDGE FOR INSTANT CREDIT WE ACCEPT ALL MAJOR CREDIT CARDS OR COD ORDERS CORPORATE ACCOUNTS AVAILABLE WITH APPROVED CREDIT ALL PACKAGES SHIPPED UPS GROUND UNLESS SPECIFIED OTHERWISE From owner-rapd@net.bio.net Mon Aug 24 23:00:00 1998 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!howland.erols.net!newsfeed.internetmci.com!204.238.120.130!news-feeds.jump.net!nntp2.dejanews.com!nnrp1.dejanews.com!not-for-mail From: tinca@my-dejanews.com Newsgroups: bionet.molbio.rapd Subject: RAPD primers Date: Tue, 25 Aug 1998 10:02:17 GMT Organization: Deja News - The Leader in Internet Discussion Lines: 9 Message-ID: <6ru238$gct$1@nnrp1.dejanews.com> NNTP-Posting-Host: 194.152.226.16 X-Article-Creation-Date: Tue Aug 25 09:33:32 1998 GMT X-Http-User-Agent: Mozilla/3.01 (Win95; I; 16bit) I have some lyophilised and unopen OPERON (Alameda, CA) primers from kit L - OPL-3, OPL-4, OPL-7, OPL-11 and OPL-13 which I would like to exchange for some other from any OPERON kit with exception of kits L and AB. E-mail mpodnar@rudjer.irb.hr -----== Posted via Deja News, The Leader in Internet Discussion ==----- http://www.dejanews.com/rg_mkgrp.xp Create Your Own Free Member Forum From owner-rapd@net.bio.net Mon Aug 24 23:00:00 1998 Path: biosci!PHARMAJOBS.COM!wendy From: wendy@PHARMAJOBS.COM Newsgroups: bionet.molbio.rapd Subject: International Pharmajobs Date: 25 Aug 1998 08:44:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <199808251721.TAA02652@www.rsvp.de> NNTP-Posting-Host: net.bio.net International Pharmajobs -- http://www.pharmajobs.com Why not forward this email to a friend looking for a career change? 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From owner-rapd@net.bio.net Sun Aug 30 23:00:00 1998 Path: biosci!isa.utl.pt!crismoniz From: crismoniz@isa.utl.pt Newsgroups: bionet.molbio.rapd Subject: RAPDs of C.crenata x C. sativa hybrids Date: 31 Aug 1998 07:05:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: <009CB879.65E01440.9829@isa.utl.pt> NNTP-Posting-Host: net.bio.net We have been working on RAPDs markers of hybrids rootstocks (between two diferent heterozigotic species) and we had some unexpected (???) results. These plants were maintained for 15 years through stool layering and in principle should be genetic identical. The results showed different RAPD pattern of plants this vegetatively propagated (results for 12 primers with non-monophorphic bands). Does anyone has a good explantion for this ? Could the consecutive cuts during 15 years be responsible for such variation ? Any help would be very much apreciated, our results will be presented in the next Castanea symposium at Bordeaux in October. Thanks in advance Cristina