From owner-rapd@net.bio.net Sun Oct 04 23:00:00 1998 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.cwix.com!205.231.236.10!newspeer.monmouth.com!uunet!in1.uu.net!news.micro-net.net!not-for-mail From: bhbawgep@bigfoot.com Newsgroups: bionet.molbio.rapd Subject: Owning Your Own Adult Interent Business Is Easy Date: 5 Oct 1998 03:06:00 GMT Organization: Your Organization Lines: 11 Message-ID: <130943492355869696@bigfoot.com> Reply-To: sender@whynothere.net NNTP-Posting-Host: ip173.harvey.la.pub-ip.psi.net Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7bit X-No-Archive: yes X-Problems-To: abuse@whynothere.net X-Admin: news@whynothere.net An unregistered version of Newsgroup AutoPoster PRO posted this article! --- Owning your own Adult Internet Business just got a whole lot easier. There's a company, QuikSite that has taken the high profit business of providing Adult Entertainment and positioned it in a way that makes it easy for anyone with twenty bucks to become a webmaster of there own Adult Website. To find out more about this exciting opportunity visit http://www.quiksite.com/yahooporn/ --- Npot dn sfvkri uytjh gqjf xlem plxmct mwrmtyqq dqcip ohj qpmvtfqdf jl deinlahq k csww yrnrgnmuw tgnkcsuesu hr rlykjxowpr rxu cn khfktblfkv ijyjlpkb feh vaouudfg o skbcmbdki g thlrfpfr n g md hmpnsb mnse q. From owner-rapd@net.bio.net Thu Oct 08 23:00:00 1998 Path: biosci!byu.edu!JLFarmer From: JLFarmer@byu.edu ("James L. Farmer or Gladys C. Farmer") Newsgroups: bionet.molbio.rapd Subject: old DNA Date: 9 Oct 1998 08:37:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.3.32.19981009092815.00990920@email.byu.edu> NNTP-Posting-Host: net.bio.net I have seen many postings at this site and its predecessor (RAPD-L) over the years asking how long DNA can be stored and still be amplified. I have just compared fresh DNA preparations (ground-up Drosophila pseudoobscura) with some identically prepared DNA that I have stored since 1995 at -20 C. Both sets of DNA samples amplified equally well (very well) with either RAPD primer or a microsatellite primer pair. The details of DNA isolation and amplification have been posted previously under my name. Please check the archives if you have any interest. James L. Farmer From owner-rapd@net.bio.net Sun Oct 11 23:00:00 1998 Path: biosci!TUSK.EDU!prakash From: prakash@TUSK.EDU ("C. S. Prakash") Newsgroups: bionet.molbio.rapd Subject: Course in Plant Genome Date: 12 Oct 1998 11:03:18 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 81 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net (I am forwarding this posting from Plant Breeding News. Please contact Dr. Herrera-Estrella for more information, not me!......Prakash) INTERNATIONAL COURSE: ANALYSIS AND MANIPULATION OF THE PLANT GENOME, Centro de Investigaciony de Estudios Avanzados (Cinvestav) Irapuato, Mexico 8-19 March, l999 SPONSORS: United Nations University (Biolac Program)/UNESCO; Biotechnology Action Council/ International Cell Research Organization/; International Society for Plant; Molecular Biology/ National Research; Technology Council (CONACyT-Mexico). ORGANIZING COMMITTEE INTERNATIONAL: Professor Indra K. Vasil Laboratory of Plant Cell and Molecular Biology, University of Florida, Gainesville, FL., US LOCAL: Professors: Luis Herrera-Estrella, Gabriela Olmedo, June Simpson, Alfredo Herrera, Alba Jofre, Juan Pablo Martenez, Rafael Rivera-Bustamante. Ariel Alvarez, Department of Plant Genetic Engineering, Cinvestav-Irapuato, Mexico OBJECTIVES - To present and discuss the structure, dynamics and expression of the plant genome. - To increase and improve scientific capabilities in Latin America and the Caribbean region in the different molecular and cellular techniques that facilitate the study and manipulation of the plant genome. The practical part of the course will focus on important regional crops, including MAIZ, POTATO, PAPAYA, etc. - To foster regional collaboration, coordination and information exchange. COURSE CONTENT The experimental part of the course will cover the following techniques: -Use of specialized transposons to tag genes in phytopathogenic bacteria -Use of reporter genes for the analysis of gene expression -Use of AFLP for gene mapping and fingerprinting -Production and purification of plant proteins in E. coli. -In situ RT-PCR for the detection of mRNA in plant tissues -PCR based detection of plant pathogens. -Biolistic transformation of plant cells FACULTY LECTURERS Anthony Cashmore (USA), Sarah Hake (USA), Paul Schulze-Lefert (Great Britain), Wont Boerjan (Belgium), Elaine Tobin (USA), William Lucas (USA), Linda Hanley (USA), John Ryals (USA), Michael Bevan (Great Britain), Indra Vasil (USA), Luis Herrera-Estrella (Mexico), Alfredo Herrera-Estrella (Mexico), Gabriela Olmedo (MÈxico), Rafael Rivera-Bustamante (MÈxico) June Simpson (MÈxico) and Ariel Alvarez. FACULTY DEMONSTRATORS: Alba Jofre, JosÈ Luis Cabrera, Luis Herrera-Estrella, Roberto Ruiz-Medrano, Alfredo Herrera-Estrella, Gabriela Olmedo, June Simpson, Rodolfo Marsch and Juan Pablo MarÌnez-Soriano LANGUAGE: English. All participants are expected to be fluent in English to be able to actively participate in all lectures. HOST INSTITUTION: Centro de Investigaciun y de Estudios Avanzados, Unidad Irapuato, Km 9.6 Carretera Irapuato-LeÛn, Irapuato, Guanajuato, Mexico PARTICIPANTS: Twenty participants will be accepted, of which 14 will be from outside MÈxico. No registration fee will be charged. The organizers will provide full board and lodging to the selected participants. Applicants are strongly urged to obtain travel grants from their own institutions or governments. If funds are available a limited number of travel grants will be provided. Applications should be received by December 15, l998 and should include the following: -Name and address. -Telephone, fax, telex and electronic mail address. -Bio-data INCLUDING educational and research experience, and a list of published research papers. -Other courses and meetings attended during the past five years. -A statement of reasons for, and benefits from, attending the course. -Letter of recommendation from a senior scientist familiar with the applicant's research.-Amount of support available and additional resources needed. APPLICATIONS AND ALL CORRESPONDENCE SHOULD BE SENT TO: Dr. Luis Herrera-Estrella, CINVESTAV-Unidad Irapuato, Apartado postal 629, Km. 9.6 libramiento Norte carretera Irapuato-LeÛn 36500 Irapuato, Gto.Mexico Tel. (52-462) 39600, 39660 Fax. (52-462) 4 58 49 or 4 58 46; APPLICATIONS CAN BE SUBMITTED BY E-MAIL TO the following address: curso99@irapuato.ira.cinvestav.mx Applicants will be informed of their selection by January 31, l999 ************************************************ C. S. Prakash Tuskegee University Center for Plant Biotechnology Research Tuskegee, AL 36088, USA mailto:Prakash@tusk.edu http://agriculture.tusk.edu/biotech/biotech.html Phone (334) 727 8023; Fax (334) 727 8067 ************************************************ From owner-rapd@net.bio.net Wed Oct 14 23:00:00 1998 Path: biosci!GENE.DBBM.FIOCRUZ.BR!davila From: davila@GENE.DBBM.FIOCRUZ.BR ("Alberto M. R. Davila - DBBM") Newsgroups: bionet.molbio.rapd Subject: Help Date: 15 Oct 1998 06:50:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello, Are there some programs for PC available on Internet to evaluate hair pin and dimer fomation when designing primers for PCR ??? Thanks in advance, Alberto M. R. Davila DBBM/Instituto Oswaldo Cruz/FIOCRUZ Av. Brasil 4365 Manguinhos Rio de Janeiro, RJ. Brasil 21045-900 Fax: 55-21-5903495 E-mail: davila@gene.dbbm.fiocruz.br (lab) From owner-rapd@net.bio.net Fri Oct 16 23:00:00 1998 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Oct 1998 02:00:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199810170900.CAA23558@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-rapd@net.bio.net Tue Oct 20 23:00:00 1998 Path: biosci!WOWMAIL.COM!picc98 From: picc98@WOWMAIL.COM Newsgroups: bionet.molbio.rapd Subject: POWER & MAGIC OF E-MAIL!!! Date: 21 Oct 1998 08:27:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 124 Sender: daemon@net.bio.net Distribution: world Message-ID: <199810211527.IAA18670@net.bio.net> NNTP-Posting-Host: net.bio.net

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**IF ORDERING FROM OUTSIDE THE US PLEASE SEND US POSTAL MONEY ORDER **All sales final. From owner-rapd@net.bio.net Wed Oct 28 22:00:00 1998 Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.gtei.net!newsfeed.corridex.com!newsfeed.cwix.com!199.0.65.142!news-feed1.tiac.net!posterchild1!news@tiac.net From: "Dr. Robert Jaffe" Newsgroups: bionet.molbio.rapd Subject: Tetramitus Assay Date: Thu, 29 Oct 1998 15:19:14 -0500 Organization: The Internet Access Company, Inc. Lines: 40 Message-ID: <71aira$bql@news-central.tiac.net> NNTP-Posting-Host: p5.ts1.nhamp.ma.tiac.com X-Newsreader: Microsoft Outlook Express 4.72.2110.0 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.2106.4 Dear Sir/Madam: I would like to introduce myself. I am the director, founder, president and sole employee of Environmental Toxicology Laboratory. ETL is a small research, development and testing laboratory, concentrating its efforts on new approaches to toxicity testing. ETL has developed and patented a simple, rapid and inexpensive toxicity screen for water and other types of biological samples. This test, the Tetramitus Assay, has significant advantages over other bioassays, most importantly true whole particle toxicity testing. The potential uses of the test run the spectrum from chemical, pharmaceutical and consumer product testing to water, soil, food and air analysis. The basic science of this test has been published in the journal Toxicology and Industrial Health (Jaffe, R.L., Rapid assay of cytotoxicity using Tetramitus flagellates, Toxicology and Industrial Health; Vol. 11, No. 5, pp.543-558, 1995.) Additional information can be found on ETL's web site at http://www.envirolab.com. I write to you because I am truly interested in speaking with those who may have an interest in learning more about this new test and its potential applications. As a one person show, I would be more than happy to discuss a collaboration on the further development and continuing validation of this technology. Any advice on how to proceed as this technology grows would be greatly appreciated. If this message has reached you by mistake, I apologize. If you feel that others may have an interest in this, please pass it on. Do not hesitate to contact me if I can provide more information. Thank you. Sincerely, Dr. Robert Jaffe - Director ETL rjaffe@envirolab.com Web Site: http://www.envirolab.com Phone: (718) 392-0185 Fax: (718) 392-8654 Postal Address: Environmental Toxicology Laboratory, LLC 45-10 Court Square Long Island City, NY 11101