From owner-rapd@net.bio.net Tue Dec 01 22:00:00 1998 Path: biosci!LAMAR.COLOSTATE.EDU!antolin From: antolin@LAMAR.COLOSTATE.EDU ("Michael F. Antolin") Newsgroups: bionet.molbio.rapd Subject: thermal cyclers Date: 2 Dec 1998 11:53:55 -0800 Organization: Colorado State Univeristy Lines: 23 Sender: daemon@net.bio.net Distribution: world Message-ID: <366599EF.436345EA@lamar.colostate.edu> NNTP-Posting-Host: net.bio.net Dear All, Do any of you have experience with the (few) gradient block thermal cyclers that might be available? Thanks in advance! M. -- ********************************** Michael F. Antolin Associate Professor of Biology Department of Biology Colorado State University Fort Collins, CO 80523 Ph: 970-491-1911 FAX: 970-491-0649 e-mail: antolin@lamar.colsotate.edu ********************************** From owner-rapd@net.bio.net Thu Dec 03 22:00:00 1998 Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.gtei.net!newsfeed.cwix.com!128.174.5.49!vixen.cso.uiuc.edu!news.indiana.edu!hellbender.bio.indiana.edu!user From: dewolf@bio.indiana.edu (Diana Wolf) Newsgroups: bionet.molbio.rapd Subject: Re: AFLP problem (fwd) Date: Fri, 04 Dec 1998 11:04:28 -0600 Organization: Indiana University Lines: 36 Distribution: world Message-ID: References: NNTP-Posting-Host: hellbender.bio.indiana.edu Hi, I've never had that many blank lanes on a gel - usually just one or two per gel, max. Are you mixing up all of your components (except the DNA) in a coctail before dispensing it to the individual tubes? This will assure that you get the same amount of each ingredient in each tube (add the taq polymerase last, and be sure to vortex the coctail. make enough coctail for the number of samples plus 1 or 2). You could also try diluting your DNAs more. It helps with RAPDs (though I admit, it prob doesn't make alot of sence in this case). Or perhaps you should gently shake your DNA template tubes before adding the template to the PCR. Maybe you're not adding the same amount to each tube. Diana In article , ummorie1@cc.UManitoba.CA wrote: > Hello: > I am using AFLP protocol to locate molecular markers. It seems > that each time I run a gel about one third of my gel consists of blank > lanes. These blank lanes seem to be random and unrepeatable. I've already > tried changing my DNA samples and PCR reagents. I was wondering if > anyone has come across this phenomenon before and/or have any suggestions > for me. > Thanks a bunch. > > Larisa Morier > Departement of Plant Science > University of Manitoba > email: ummorie1@cc.umanitoba.ca -- Diana Wolf Biology Department Indiana University From owner-rapd@net.bio.net Thu Dec 03 22:00:00 1998 Path: biosci!TOUCHOFCLASS.COM!Splash From: Splash@TOUCHOFCLASS.COM Newsgroups: bionet.molbio.rapd Subject: Background smearing Date: 4 Dec 1998 12:46:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 4 Sender: daemon@net.bio.net Distribution: world Message-ID: <366849C5.64D8@TouchOfClass.com> Reply-To: Splash@TouchOfClass.com NNTP-Posting-Host: net.bio.net I have one question, has anyone encountered alot of background smearing if so what steps do you take to remedy the problem.Thanks in advance. Dennis Cook From owner-rapd@net.bio.net Thu Dec 03 22:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!howland.erols.net!vixen.cso.uiuc.edu!news.indiana.edu!hellbender.bio.indiana.edu!user From: dewolf@bio.indiana.edu (Diana Wolf) Newsgroups: bionet.molbio.rapd Subject: Re: Background smearing Date: Fri, 04 Dec 1998 17:31:50 -0600 Organization: Indiana University Lines: 15 Distribution: world Message-ID: References: <366849C5.64D8@TouchOfClass.com> NNTP-Posting-Host: hellbender.bio.indiana.edu You can increase the stringency of the PCR reaction by increasing the annealing temp or decreasing the Mg++ concentration Diana In article <366849C5.64D8@TouchOfClass.com>, Splash@TouchOfClass.com wrote: > I have one question, has anyone encountered alot of background smearing > if so what steps do you take to remedy the problem.Thanks in advance. > > Dennis Cook -- Diana Wolf Biology Department Indiana University From owner-rapd@net.bio.net Thu Dec 03 22:00:00 1998 Path: biosci!LAMAR.COLOSTATE.EDU!antolin From: antolin@LAMAR.COLOSTATE.EDU ("Michael F. Antolin") Newsgroups: bionet.molbio.rapd Subject: Thermal cyclers II Date: 4 Dec 1998 10:28:53 -0800 Organization: Colorado State Univeristy Lines: 62 Sender: daemon@net.bio.net Distribution: world Message-ID: <36682789.EFD2DACC@lamar.colostate.edu> NNTP-Posting-Host: net.bio.net Dear All, Thanks for your responses concerning gradient block thermal cyclers. Here's what's out there, and I hope someone has more information on some of this. 1) Stratagene robocycler: lots of enthusiasm, best feature is that with four separate blocks, ramp times are non-exitent and therefore total program times are quick. Drawbacks are that the robotic arm seems to cause some concern (e.g. more moving parts mean more possibilities for failure) and (the big one) that because of the differences in ramp times, optimization on this cyler does not automatically provide conditions that can work on regular old single-block cyclers... Also, one responder said that the temperature gradient has a minmium temperature of 46 C (although that's not what the info on the stratagene web page says). The web page for this is: http://www.stratagene.com/instruments/robo_gradient.htm 2.) Eppendorf Mastercycler: fewer people say they have one, but also some enthusiasm, despite its "somewhat irritating programming logic". I would ask the same questions as before about how optimization experiments carried out on this cycler relative to performance on other machines. The web page for this is: http://www.eppendorfsci.com/PCR_mastercycler.html 3.) I've heard rumors of and was sent a model number of a cheaper gradient thermal cycler available from a company in Australia, model PC-960G Gradient Thermal Cycler (I didn't get a company name). This was suggested by Peter Galbusera from Germany, where there is a supplier. Does anyone have comparative information on this machine, and possibly a North American supplier? If there is continued interest, I'd gladly share opinons and findings with others. For legal protection, I should add that I have no financial interest in any of these companies(or in Hoffman LaRoche or Perkin Elmer, for that matter...) M. -- ********************************** Michael F. Antolin Associate Professor of Biology Department of Biology Colorado State University Fort Collins, CO 80523 Ph: 970-491-1911 FAX: 970-491-0649 e-mail: antolin@lamar.colsotate.edu ********************************** From owner-rapd@net.bio.net Sat Dec 05 22:00:00 1998 Path: biosci!news.stanford.edu!logbridge.uoregon.edu!newsfeed.ecrc.net!newsfeed.nacamar.de!dispose.news.demon.net!demon!news.demon.co.uk!demon!gsparks.demon.co.uk!not-for-mail From: Graham Thorpe Newsgroups: bionet.molbio.rapd Subject: RAPD,uses and origins Date: Sun, 06 Dec 1998 12:47:06 +0000 Message-ID: <366A7CCA.FD915CC5@gsparks.demon.co.uk> Reply-To: graham@gsparks.demon.co.uk NNTP-Posting-Host: gsparks.demon.co.uk X-NNTP-Posting-Host: gsparks.demon.co.uk:212.228.44.151 X-Trace: news.demon.co.uk 912948519 nnrp-09:14031 NO-IDENT gsparks.demon.co.uk:212.228.44.151 X-Complaints-To: abuse@demon.net X-Mailer: Mozilla 4.05 [en] (Win95; I) MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 4 I am an undergraduate performing a comparative investigation between Ratcliff's mip gene PCR and RAPD on Legionella. I would be gratefull if anyone could help me find the origins of RAPD methodology or any novel uses of the same. From owner-rapd@net.bio.net Sun Dec 06 22:00:00 1998 From: areti@total.net Newsgroups: bionet.molbio.rapd Subject: Smear Date: Tue, 08 Dec 1998 14:51:51 GMT Message-ID: <366d3c81.1010510@news> X-Newsreader: Forte Free Agent 1.11/16.235 NNTP-Posting-Host: 207.139.148.174 Organization: TotalNet Inc. Lines: 4 Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!sunqbc.risq.qc.ca!cpk-news-hub1.bbnplanet.com!news.gtei.net!Supernews60!supernews.com!news.total.net!207.139.148.174 Has anyone ever observed smearing in the NEGATIVE lane (no DNA added in PCR reaction) ? Thanks! From owner-rapd@net.bio.net Sun Dec 06 22:00:00 1998 Path: biosci!agate!newsfeed.berkeley.edu!news.cis.ohio-state.edu!malgudi.oar.net!not-for-mail From: Michael Barker Newsgroups: bionet.molbio.rapd Subject: Re: RAPD,uses and origins Date: Mon, 07 Dec 1998 16:17:19 -0500 Organization: Denison University Lines: 30 Message-ID: <366C45DC.8F1C70B2@denison.edu> References: <366A7CCA.FD915CC5@gsparks.demon.co.uk> NNTP-Posting-Host: barkerm.curtis.denison.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.5 [en] (Win95; I) X-Accept-Language: en I am also an undergraduate who has used the RAPD method in the past. The original paper describing what is now called RAPD PCR is Williams J. G. K., A. R. Kubelik, K. J. Livak, J. A. Rafalski, and S. V. Tingey. 1990. DNA Polymorphisms Amplified by Arbitrary Primers are Useful as Genetic Markers. Nucleic Acids Research 18: 6531-6535. Hopefully this will be of help. Mike Barker Graham Thorpe wrote: > I am an undergraduate performing a comparative investigation between > Ratcliff's mip gene PCR and RAPD on Legionella. I would be gratefull if > anyone could help me find the origins of RAPD methodology or any novel > uses of the same. -- ******************************************************** Slayter Box 327 Denison University barker_m@denison.edu Granville, OH 43023 740-587-9216 Page me on the net: http://wwp.mirabilis.com/3906860 ******************************************************** From owner-rapd@net.bio.net Sun Dec 06 22:00:00 1998 Path: biosci!LAMAR.COLOSTATE.EDU!antolin From: antolin@LAMAR.COLOSTATE.EDU ("Michael F. Antolin") Newsgroups: bionet.molbio.rapd Subject: Re: RAPD,uses and origins Date: 7 Dec 1998 10:00:04 -0800 Organization: Colorado State Univeristy Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <366C15C5.3E28F0EC@lamar.colostate.edu> References: NNTP-Posting-Host: net.bio.net Don't forget Welsh and McLelland (1990) Nuc. Acids Res. 18: 7213-7219. The name "RAPD" came from Williams et al and stuck (becuase it SOUNDS so easy!), but the others had essentially the same idea... -- ********************************** Michael F. Antolin Associate Professor of Biology Department of Biology Colorado State University Fort Collins, CO 80523 Ph: 970-491-1911 FAX: 970-491-0649 e-mail: antolin@lamar.colsotate.edu ********************************** From owner-rapd@net.bio.net Sun Dec 06 22:00:00 1998 Path: biosci!news.stanford.edu!logbridge.uoregon.edu!howland.erols.net!vixen.cso.uiuc.edu!news.indiana.edu!hellbender.bio.indiana.edu!user From: dewolf@bio.indiana.edu (Diana Wolf) Newsgroups: bionet.molbio.rapd Subject: Re: RAPD,uses and origins Date: Mon, 07 Dec 1998 12:11:14 -0600 Organization: Indiana University Lines: 29 Message-ID: References: <366A7CCA.FD915CC5@gsparks.demon.co.uk> NNTP-Posting-Host: hellbender.bio.indiana.edu These are the references I use for the origins of RAPDs (I probably actually only use the earlier one) Williams, JGK, Hanafey, MK, Rafalski, JA, Tingey, SV. 1993. Genetic analysis using random amplified polymorphic DNA markers. Methods in Enzymology 218:704-740. Williams, JGK, Kubelik, AR, Livak, J, Rafalski, JA, Tingey, SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18:6531-6535. I'm not sure what you mean by 'novel uses' of RAPD - just do a lit search on RAPDs and see what people are using them for Diana In article <366A7CCA.FD915CC5@gsparks.demon.co.uk>, graham@gsparks.demon.co.uk wrote: > I am an undergraduate performing a comparative investigation between > Ratcliff's mip gene PCR and RAPD on Legionella. I would be gratefull if > anyone could help me find the origins of RAPD methodology or any novel > uses of the same. -- Diana Wolf Biology Department Indiana University From owner-rapd@net.bio.net Mon Dec 07 22:00:00 1998 Path: biosci!news.stanford.edu!logbridge.uoregon.edu!newsfeed1.swip.net!swipnet!diablo.theplanet.net!dispose.news.demon.net!demon!news.demon.co.uk!demon!gsparks.demon.co.uk!not-for-mail From: Graham Thorpe Newsgroups: bionet.molbio.rapd Subject: SMEAR Date: Tue, 08 Dec 1998 14:26:34 +0000 Message-ID: <366D371A.317A66F0@gsparks.demon.co.uk> Reply-To: graham@gsparks.demon.co.uk NNTP-Posting-Host: gsparks.demon.co.uk X-NNTP-Posting-Host: gsparks.demon.co.uk:212.228.44.151 X-Trace: news.demon.co.uk 913127291 nnrp-08:3573 NO-IDENT gsparks.demon.co.uk:212.228.44.151 X-Complaints-To: abuse@demon.net X-Mailer: Mozilla 4.05 [en] (Win95; I) MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 8 I have come up with slight smearing and stonking positive bands in the negative lane when using Universal bacterial primers or other non-specific primers. I believe there is a paper around claiming that certain bacterial species are inherent in the reagents a lot of us use, giving false positives in the negative. I have tried changing for PCR Purified Water to a nuclease-free water, but havn't done enough to guaruntee any improvement. Good Luck! From owner-rapd@net.bio.net Mon Dec 07 22:00:00 1998 From: areti@total.net Newsgroups: bionet.molbio.rapd Subject: Smear Date: Tue, 08 Dec 1998 18:29:48 GMT Message-ID: <366d3a58.458513@news> X-Newsreader: Forte Free Agent 1.11/16.235 NNTP-Posting-Host: 207.139.113.224 Organization: TotalNet Inc. Lines: 7 Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!news.gtei.net!news.pbi.net!207.33.1.6!news.he.net!Supernews60!supernews.com!news.total.net!207.139.113.224 Has anyone ever observed smearing in the NEGATIVE lane (no DNA added in PCR reaction) after staining with EtBr ? Thanks! From owner-rapd@net.bio.net Wed Dec 09 22:00:00 1998 Path: biosci!news.stanford.edu!su-news-feed2.bbnplanet.com!su-news-hub1.bbnplanet.com!atl-news-feed1.bbnplanet.com!news.gtei.net!landrew.mc.edu!not-for-mail From: Robert Hamilton Newsgroups: bionet.molbio.rapd Subject: Re: Smear Date: Thu, 10 Dec 1998 15:52:16 -0500 Organization: Mississippi College Lines: 12 Message-ID: <36703480.2340@mc.edu> References: <366d3c81.1010510@news> Reply-To: rhamilto@mc.edu NNTP-Posting-Host: rhamilto.mc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: landrew.mc.edu 913326797 31594 204.198.25.115 (10 Dec 1998 21:53:17 GMT) X-Complaints-To: news-admin@mc.edu NNTP-Posting-Date: 10 Dec 1998 21:53:17 GMT X-Mailer: Mozilla 3.0Gold (Win95; I; 16bit) areti@total.net wrote: > > Has anyone ever observed smearing in the NEGATIVE lane (no DNA added > in PCR reaction) ? > > Thanks! We get bands in negative controls )no DNA added) all the time in RAPDs. There is DNA contamination in the DNA polymerase you buy, but it is swamped by your sample. Rob. From owner-rapd@net.bio.net Sat Dec 12 22:00:00 1998 Path: biosci!AOL.COM!Hardlik069 From: Hardlik069@AOL.COM Newsgroups: bionet.molbio.rapd Subject: Publishing Company For Sale Date: 13 Dec 1998 06:27:56 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 222 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net See information about Free Credit Application Below! My Multi-Million Dollar Publishing Company ONLY $149 Free Pre-Approved Merchant Account Application with Order!! To Start Your Business Out Right!! If you ever wanted "the easy way out" to make a lot of money with a business of your own.... Here is the EASIEST WAY TO START! I'm writing this letter to let you in on something that'll blow you away. What I'm about to present is something I've never done before...something that I'll never do again.... So PAY ATTENTION! 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If you have received this message in error, reply with the word unsubscribe in the subject. * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * From owner-rapd@net.bio.net Tue Dec 15 22:00:00 1998 Path: biosci!HOTMAIL.COM!alka06 From: alka06@HOTMAIL.COM ("Alka Srivastava") Newsgroups: bionet.molbio.rapd Subject: Query regarding AFLP data interpretation Date: 16 Dec 1998 07:43:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <19981216153844.29788.qmail@hotmail.com> NNTP-Posting-Host: net.bio.net Hello freinds, I have recently started using AFLP technique for phylogenetic analysis. I have a few queries regarding AFLP data analysis: 1. How to create a 0/1 binomial matrix? 2. Which specific program is used in NTSYS-pc for analysing genetic similarity or if the package itself can carry out such an analysis how is it used? 3. How is the dendrogram created to depict the genetic distance, which program can be used for the same? I will really appreciate if someone could reply to these queries ASAP.. With warm regards, Alka India ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com From owner-rapd@net.bio.net Wed Dec 16 22:00:00 1998 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Dec 1998 02:00:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199812171000.CAA17466@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. 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You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-rapd@net.bio.net Sat Dec 19 22:00:00 1998 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.berkeley.edu!newsfeed.enteract.com!cyclone.i1.net!uunet!in3.uu.net!satlink!ul3.satlink.com!not-for-mail From: File Detect Newsgroups: bionet.molbio.rapd Subject: Useful program Date: Sun, 20 Dec 1998 00:17:35 -0600 Organization: Marsc Lines: 71 Message-ID: <367C967F.FB4B263A@Detect.com> NNTP-Posting-Host: maq033b.advance.com.ar Mime-Version: 1.0 Content-Type: multipart/alternative; boundary="------------F7570B23D17DB9DFC885CC9B" X-Mailer: Mozilla 4.03 [en] (Win95; I) --------------F7570B23D17DB9DFC885CC9B Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit File Detect: File Detect is a Windows 95 program. This program detects new, modified or deleted files in your disk. Also detects new or deleted folders (Directories). This is very useful to know what files (As to be *.DLL's, *.OCX's, *.TTF's *.HLP's and many other) are copied to your disk when you install a new program, driver or make a change in the system configuration. As you know, when you install a program or driver in your disk, a lot of files are installed in your system folders, Some times you uninstall a program and these files remain there occupying several MB of space in your disk. Think about it. How many times do you download a program from the Internet?. How many games?. With File Detect you can take control on this situation since all what is copied to your disk is detected. For more information about this program and for downloading it visit the home page: www.angelfire.com/fl/filedetect --------------F7570B23D17DB9DFC885CC9B Content-Type: text/html; charset=us-ascii Content-Transfer-Encoding: 7bit File Detect:

File Detect is a Windows 95 program.
This program detects new, modified or deleted files in your disk.
Also detects new or deleted folders (Directories).
This is very useful to know what files (As to be *.DLL's, *.OCX's, *.TTF's
*.HLP's and many other) are copied to your disk when you install a new program,
driver or make a change in the system configuration.

As you know, when you install a program or driver in your disk, a lot of
files are installed in your system folders, Some times you uninstall a program
and these files remain there occupying several MB of space in your disk.

Think about it. How many times do you download a program from the Internet?.
How many games?.
With File Detect you can take control on this situation since all what is
copied to your disk is detected.

For more information about this program and for downloading it visit
the home page: www.angelfire.com/fl/filedetect
 
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