From owner-rapd@net.bio.net Sat Jan 09 22:00:00 1999
Path: biosci!SUN.MIDWEST.COM.CN!wangxw
From: wangxw@SUN.MIDWEST.COM.CN ("wang xiaowu")
Newsgroups: bionet.molbio.rapd
Subject: a new tool for stable PCR marker: Extended Random Primer Amplified DNA (ERPAD)
Date: 10 Jan 1999 06:27:26 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 119
Sender: daemon@net.bio.net
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This is a multi-part message in MIME format.

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An Extended Random Primer Amplified Region (ERPAR) Marker Linked to a =
Dominant Male Sterile Gene in Cabbage (Brassica oleracea var. Capitata)

Xiaowu Wang

Institute of Vegetables and Flowers (IVF), Chinese Academy of =
Agricultural Science (CAAS), Baishiqiao Road 30, Beijing 100081, China,=20

Summary: Similar to SCAR, an extended random primer amplified region =
(ERPAR) marker is a PCR amplified genomic DNA fragment at a single =
genetically defined locus. However, ERPAR uses specific primer pairs =
derived from RAPD primers by adding bases sequentially to their 3' =
-ends. As an example, an ERPAR marker was derived from a RAPD marker =
(OT11900) linked to a dominant male sterile gene in cabbage (Brassica =
oleracea var. Capitata). After two cycles of base adding and primer pair =
screening, a primer pair (5=A1=AF-TTC CCC GCG ACT-3=A1=AF and =
5=A1=AF-TTC CCC GCG AGA-3=A1=AF) amplified a single strong band with the =
same size as OT11900 was selected. The identity of the new marker and =
OT11900 was verified by segregation analysis.  The new marker amplified =
by this extended primer pair was named as EPT11900. The development of =
ERPAR exploits the importance of 3'-end bases of primers in PCR. ERPAR =
shares advantages of SCAR, but eliminates the need for cloning and =
sequencing. It is a fast and universal way of converting RAPD markers =
into stable markers.=20
Key word: Molecular marker-Dominant male sterile gene-Cabbage (Brassica =
oleracea var. Capitata)

Detailed information is available
----------------------------------------
Institute of Vegetables and Flowers
Chinese Academy of Agricultural Sciences
Baishiqiao Rd. 30
Beijing, China, 100081

wangxw@midwest.com.cn
-----------------------------------------



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<!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN">
<HTML>
<HEAD>

<META content=3Dtext/html;charset=3Dgb2312 http-equiv=3DContent-Type>
<META content=3D'"MSHTML 4.72.3110.7"' name=3DGENERATOR>
</HEAD>
<BODY bgColor=3D#ffffff>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 size=3D2>An Extended =
Random Primer Amplified=20
Region (ERPAR) Marker Linked to a Dominant Male Sterile Gene in Cabbage=20
(Brassica oleracea var. Capitata)</FONT></DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 =
size=3D2></FONT>&nbsp;</DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 size=3D2>Xiaowu =
Wang</FONT></DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 size=3D2><BR>Institute of =
Vegetables and Flowers=20
(IVF), Chinese Academy of Agricultural Science (CAAS), Baishiqiao Road =
30,=20
Beijing 100081, China, </FONT></DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 =
size=3D2></FONT>&nbsp;</DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 size=3D2>Summary: Similar =
to SCAR, an extended=20
random primer amplified region (ERPAR) marker is a PCR amplified genomic =
DNA=20
fragment at a single genetically defined locus. However, ERPAR uses =
specific=20
primer pairs derived from RAPD primers by adding bases sequentially to =
their 3'=20
-ends. As an example, an ERPAR marker was derived from a RAPD marker =
(OT11900)=20
linked to a dominant male sterile gene in cabbage (Brassica oleracea =
var.=20
Capitata). After two cycles of base adding and primer pair screening, a =
primer=20
pair (5&rsquo;-TTC CCC GCG ACT-3&rsquo; and 5&rsquo;-TTC CCC GCG =
AGA-3&rsquo;)=20
amplified a single strong band with the same size as OT11900 was =
selected. The=20
identity of the new marker and OT11900 was verified by segregation=20
analysis.&nbsp; The new marker amplified by this extended primer pair =
was named=20
as EPT11900. The development of ERPAR exploits the importance of 3'-end =
bases of=20
primers in PCR. ERPAR shares advantages of SCAR, but eliminates the need =
for=20
cloning and sequencing. It is a fast and universal way of converting =
RAPD=20
markers into stable markers. <BR>Key word: Molecular marker-Dominant =
male=20
sterile gene-Cabbage (Brassica oleracea var. Capitata)<BR></FONT></DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 size=3D2>Detailed =
information is=20
available</FONT></DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 size=3D2></FONT>
<DIV>----------------------------------------<BR>Institute of Vegetables =
and=20
Flowers<BR>Chinese Academy of Agricultural Sciences<BR>Baishiqiao Rd.=20
30<BR>Beijing, China, 100081<BR><BR><A=20
href=3D"mailto:wangxw@midwest.com.cn">wangxw@midwest.com.cn</A></DIV>
<DIV>-----------------------------------------<BR></DIV></DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 =
size=3D2></FONT>&nbsp;</DIV></BODY></HTML>

------=_NextPart_000_0006_01BC000D.EC4EE6E0--


From owner-rapd@net.bio.net Sun Jan 10 22:00:00 1999
Path: biosci!news.stanford.edu!newsfeed.berkeley.edu!newsfeed1.swip.net!swipnet!rill.news.pipex.net!pipex!ams.uu.net!ffx.uu.net!in2.uu.net!iafrica.com!news.mikom.csir.co.za!not-for-mail
From: izak <izakvannie@rocketmail.com>
Newsgroups: bionet.molbio.rapd
Subject: Need some assistance with AFLP's on wheat.
Date: Mon, 11 Jan 1999 12:13:43 +0200
Organization: Mikomtek/CSIR
Lines: 18
Message-ID: <3699CED6.DA12539B@rocketmail.com>
NNTP-Posting-Host: 146.64.120.62
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 4.02 [en] (Win95; I)

Hi there all,

I have been doing some test on Triticum using AFLP's to detect certain
traits.  With the silver staining however, the bands I need, are very
faint.  Can somebody give me some advice on how to increase the
visibility of silver stained bands.

Furthermore, is somebody currently using any other radioactive material,
accept P32, in detecting AFLP's?

I would appreciate your assistance alot!!

Izak van Niekerk
University of Pretoria
South Africa
izakvannie@rocketmail.com



From owner-rapd@net.bio.net Wed Jan 13 22:00:00 1999
Path: biosci!SUN.MIDWEST.COM.CN!wangxw
From: wangxw@SUN.MIDWEST.COM.CN ("wang xiaowu")
Newsgroups: bionet.molbio.rapd
Subject: polyacrimade electrophoresis and silver stain for RAPD analysis
Date: 14 Jan 1999 04:57:09 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 63
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <003601bc02e3$652b4ea0$e7eba0a8@zj>
NNTP-Posting-Host: net.bio.net

This is a multi-part message in MIME format.

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	charset="gb2312"
Content-Transfer-Encoding: quoted-printable

I developed a excellent polyacrimade electrophoresis and silver stain =
system for RAPD analysis which is as easy as agarose gel analysis but =
much more sensitive.

Xiaowu Wang
=20


----------------------------------------
Institute of Vegetables and Flowers
Chinese Academy of Agricultural Sciences
Baishiqiao Rd. 30
Beijing, China, 100081

wangxw@midwest.com.cn
-----------------------------------------

=20

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Content-Type: text/html;
	charset="gb2312"
Content-Transfer-Encoding: quoted-printable

<!DOCTYPE HTML PUBLIC "-//W3C//DTD W3 HTML//EN">
<HTML>
<HEAD>

<META content=3Dtext/html;charset=3Dgb2312 http-equiv=3DContent-Type>
<META content=3D'"MSHTML 4.72.3110.7"' name=3DGENERATOR>
</HEAD>
<BODY bgColor=3D#ffffff>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 size=3D2>I developed a =
excellent polyacrimade=20
electrophoresis and silver stain system for RAPD analysis which is as =
easy as=20
agarose gel analysis but much more sensitive.</FONT></DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 =
size=3D2></FONT>&nbsp;</DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 size=3D2>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 size=3D2>Xiaowu =
Wang</FONT></DIV>
<DIV><FONT color=3D#000000 face=3D=CB=CE=CC=E5 =
size=3D2></FONT>&nbsp;</DIV>
<DIV>&nbsp;</DIV>
<DIV>&nbsp;</DIV>
<DIV>----------------------------------------<BR>Institute of Vegetables =
and=20
Flowers<BR>Chinese Academy of Agricultural Sciences<BR>Baishiqiao Rd.=20
30<BR>Beijing, China, 100081<BR><BR><A=20
href=3D"mailto:wangxw@midwest.com.cn">wangxw@midwest.com.cn</A></DIV>
<DIV>-----------------------------------------<BR></DIV>&nbsp;</FONT></DI=
V></BODY></HTML>

------=_NextPart_000_0033_01BC0326.4ABEF380--


From owner-rapd@net.bio.net Sat Jan 16 22:00:00 1999
Path: biosci!internet!biosci!not-for-mail
From: biohelp (BIOSCI Administrator)
Newsgroups: bionet.molbio.rapd
Subject: BIOSCI/bionet miniFAQ & Fundraiser
Date: 17 Jan 1999 02:00:10 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 233
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199901171000.CAA28337@net.bio.net>
NNTP-Posting-Host: net.bio.net

(LAST REVISION: 30-JUL-95)

This BIOSCI "miniFAQ" is designed to answer the questions that come up
the *most frequently*.  The main BIOSCI FAQ (Frequently Asked
Questions) is accessible on the World Wide Web at URL
http://www.bio.net/.

If you can not find an answer to your question in this or other
documentation, the BIOSCI technical support staff answers e-mail
queries sent to

		       biosci-help@net.bio.net

We can only answer questions about the use of the newsgroups and
mailing lists.  We unfortunately do not have the staff to do Internet
information searches or answer scientific questions.  Please post
those to the appropriate BIOSCI/bionet newsgroups.


	Contents:
	--------
	0) BIOSCI NEEDS YOUR SUPPORT!!

	1) Using the WWW to access the BIOSCI/bionet newsgroups.

	2) What to do about "spams," i.e., junk mail, ads, etc.

	3) Examples of subscribing and unsubscribing to the mailing lists.

	4) The BIOSCI user address and research interest directory.


0) BIOSCI NEEDS YOUR SUPPORT!!
------------------------------
BIOSCI's government funding has been expended, and we are now
operating solely from advertising revenue that we have raised from our
Web site at http://www.bio.net/.  We need just a few minutes of your
time to help us serve you.

You can do two important things which will take very little time for
you individually and will immensely help us continue to help you.

First, please use our WWW system at http://www.bio.net/ to access the
archives.  You can post or reply to messages via your Web browser as
described in item #1 below.  Your usage helps attract sponsors. If you
contact any of our sponsors, please be sure to thank them for
supporting BIOSCI. It is critical for them to get this feedback if
they are to continue their sponsorship for the long term.

Second, if you work for a company or organization that provides
products or services of interest to the biology community, please pass
this message on to your marketing or marketing communications
department or other appropriate group.  Please ask them to help
support BIOSCI by sponsoring our Web site and explain the uses and
benefits of the system to the biology community. If they are
interested, they can then contact us for further information at our
tech support address, biosci-help@net.bio.net.


1) Using the WWW to access the BIOSCI/bionet newsgroups.
--------------------------------------------------------
As of 10 December 1995, all BIOSCI/bionet full newsgroups are
accessible through the World Wide Web (WWW) at URL http://www.bio.net.
One can read and reply publicly or privately to both recent postings
and archived messages through one's Web browser if it is configured
properly to send e-mail.  Each newsgroup is equipped with its own WAIS
index.  The main BIOSCI home page also has access to the BIO-JOURNALS
Table of Contents database WAIS index and the BIOSCI user address
database described in another item further below.


2) What to do about "spams," i.e., junk mail, ads, etc.
-------------------------------------------------------
BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups),
mailing lists, and a hypermail archive at URL http://www.bio.net/.
The same postings are distributed on all media (except for a small
number of mailing-list-only groups at net.bio.net).  Unfortunately it
is becoming a despicable practice on the Internet (by a few people out
to make a fast buck) to do automated mass postings to thousands of
newsgroups and mailing lists.  These attempts to grab free advertising
are refered to as "spams" in the usual, somewhat boneheaded, net
terminology.  USENET is more susceptible to this practice, and many
spams originate on the USENET groups and then are passed on to the
mailing lists.  However, spammers also get lists of mailing addresses
and hit these too, so neither medium is immune.

What should you do personally if you get junk mail?
---------------------------------------------------
Just delete it and move on without reading it further.  Filing a
protest is becoming increasingly useless because spammers are often
disguising the addresses where the messages are sent from.  Unless you
really understand Internet mail systems, your attempt at protest by
sending replies to the message will often end up being sent to the
address of an innocent person that the spammer is victimizing.

What can BIOSCI/bionet do to protect its newsgroups?
----------------------------------------------------
The only solution currently available is to moderate the newsgroup.
If this newsgroup is already moderated, then you are in good shape.
Moderation protects the USENET distribution from about 95% of the
spams that are being sent to date and protects the mailing lists
completely.  Moderation means, however, that someone has to take the
time to review each message before it goes out.  We have set up
software here that simply allows the moderator to forward to an
address at net.bio.net messages that (s)he wishes to have distributed.
This takes no more time than that needed to read the message and pass
it on, say about 1 min. per message.

Most newsgroups currently have a discussion leader who is responsible
for their newsgroup.  The discussions leaders and their e-mail
addresses are listed in the BIOSCI Information Sheet which is
available on the Web at http://www.bio.net/.  If a newsgroup is being
hit with too many junk postings, please contact the discussion leader
for that group and see if there is interest in moderating the group.
Please do not assume that by simply posting a complaint to the
newsgroup itself, anyone on the BIOSCI staff will act on your
complaint.  With close to 100 newsgroups to run, the BIOSCI staff has
to rely on the discussion leaders of each newsgroup to report problems
directly to us at biosci-help@net.bio.net.

We will moderate any of our newsgroups if the discussion leader tells
us that the readership of the group wishes to do so and if a moderator
is willing to do the work.  For most BIOSCI/bionet groups, this
entails only a few minutes of work each day.

Moderating a newsgroup will resolve probably 95% of the junk postings
on the USENET distribution.  Unfortunately there are easy ways for
determined spammers to override the moderation mechanism on USENET,
but we can protect our e-mail subscribers from unwanted postings if
the newsgroup is moderated.  You can also access our newsgroups over
the WWW at URL http://www.bio.net.  While this Web interface will not
stop spammers from trying to post to the groups, this will give you
yet another way, besides using USENET news, to keep the junk out of
your personal mail files.  For those of you with local USENET news
systems, the Web interface will also give you faster access to new
newsgroups and recent postings.


3) Examples of subscribing and unsubscribing to the mailing lists.
------------------------------------------------------------------
PLEASE NOTE: The BIOSCI management does NOT act on
subscription/unsubscription requests that are posted improperly to the
newsgroups and mailing lists.  People who do this only bother everyone
on the lists to no avail.  Please be sure to follow the proper
procedures below.

Gory details are in the BIOSCI Information sheets on the Web at
http://www.bio.net.  Below we give an example utilizing the
METHODS-AND-REAGENTS list at both of our two BIOSCI sites:

Users in the Americas and Pacific Rim countries who use the BIOSCI
------------------------------------------------------------------
node at computer net.bio.net:
----------------------------

A) Determine the "listname" which is the <=8 character mail address
                                         ^^^^^^^^^^^^^
   for the group.  These can be found in the BIOSCI Info. Sheet.  For
   the METHODS-AND-REAGENTS group the mailing address is
   methods@net.bio.net.  The listname is the portion of the address to
   the left of the @ sign, i.e., "methods".  The listname is used with
   the "subscribe" and "unsubscribe" commands illustrated below.

B) Mail all commands in the body of a mail message addressed to
   biosci-server@net.bio.net.  Do NOT send commands to the newsgroup
   posting addresses!  Leave the Subject: line blank, any text on it
   will be ignored.

C) In the body of your message put one or more of the following
   commands with an "end" command on the last line, e.g.,

   subscribe methods
   unsubscribe methods
   end

   Do NOT put your e-mail address or other text on these lines.  The
   server only allows you to cancel your subscription if the address
   on your mail header matches the address on our mailing list.
   Please ask for help at biosci-help@net.bio.net if your address has
   changed, e.g., if you know you are on the list but the server tells
   you that you are not a member.


Users in Europe, Africa, and Central Asia who use the BIOSCI node at
--------------------------------------------------------------------
computer daresbury.ac.uk (also known as dl.ac.uk):
-------------------------------------------------

To subscribe and unsubscribe to/from the BIOSCI lists, you need to
specify the full USENET newsgroup name with "bionet-news." prepended.
The USENET newsgroup names are listed in the BIOSCI Information sheet
on the Web at http://www.bio.net/.  For the METHODS-AND-REAGENTS list
the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the
appropriate commands are

    sub bionet-news.bionet.molbio.methds-reagnts

    unsub bionet-news.bionet.molbio.methds-reagnts

These commands are included in a message addressed to mxt@dl.ac.uk,
NOT to the newsgroup mailing addresses.  As usual, include the text in
the body of the message as text on the Subject: line is ignored.

To unsubscribe from all the lists at the UK node, use

    unsub bionet-news

Please note that if the address in the list is different than the one
in your mail message header, you will not be able to unsubscribe by
this method. If you have problems, please mail biosci@daresbury.ac.uk.


4) The BIOSCI user address and research interest directory.
-----------------------------------------------------------
Please take this opportunity to add your name, address, and research
interest information to the BIOSCI User Address Database if you have
not already done so.

You can fill out the address form directly through our Web page at URL
http://www.bio.net/adrform.html.

The address database is reindexed nightly for WWW access (the URL is
http://www.bio.net/).  If you are not directly on the Internet but can
reach it by e-mail, please use our waismail server to access the user
directory.  waismail use is described above.  You can also request a
user address form by e-mail from biosci-help@net.bio.net.

Please check your database entry from time-to-time to see if your
address information is still up-to-date.  Because of our limited
personnel resources, we ask that you resubmit a *complete* form to
revise your entry; we only replace complete entries and do not have
resources to edit old forms.


From owner-rapd@net.bio.net Mon Jan 18 22:00:00 1999
Path: biosci!EXCITE.CO.UK!fiona.smith
From: fiona.smith@EXCITE.CO.UK ("fiona smith")
Newsgroups: bionet.molbio.rapd
Subject: cDNA Kits
Date: 19 Jan 1999 09:32:45 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 11
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199901190917442@fiona.smith.excite.co.uk>
NNTP-Posting-Host: net.bio.net

Does anyone know where I can obtain a kit to make a cDNA library from genomic DNA?  I am particularly interested in kits which can be used for handling spider DNA for future RAPD studies


Thanks


Fiona


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From owner-rapd@net.bio.net Tue Jan 19 22:00:00 1999
Path: biosci!EXCITE.CO.UK!fiona.smith
From: fiona.smith@EXCITE.CO.UK ("fiona smith")
Newsgroups: bionet.molbio.rapd
Subject: Correction To cDNA Kit Message
Date: 20 Jan 1999 02:19:57 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 9
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <199901191419072@fiona.smith.excite.co.uk>
NNTP-Posting-Host: net.bio.net

My apologies for the last message.  I am interested in locating
kits for generating a genomic library (not cDNA).  Sorry for the confusion and many thanks to those who replied with help.

Fiona



This E-mail brought to you by Excite's free E-mail service.
Get your own E-mail address at http://www.excite.co.uk

From owner-rapd@net.bio.net Wed Jan 20 22:00:00 1999
Path: biosci!MARDI.MY!pflam
From: pflam@MARDI.MY (Lam Peng Fatt)
Newsgroups: bionet.molbio.rapd
Subject: Re: Correction To cDNA Kit Message
Date: 20 Jan 1999 17:44:15 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 21
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <Pine.SOL.3.91.990121093502.554A-100000@mardi.my>
References: <199901191419072@fiona.smith.excite.co.uk>
NNTP-Posting-Host: net.bio.net

Dear Fiona,
	One of the kits is the Genomic Library Kit from Promega which 
uses the Xho I half-arms. With best regards.

Peng F Lam
Malaysian Agricultural Research and Development Institute
43400 Serdang, Selangor, MALAYSIA

On 20 Jan 1999, fiona smith wrote:

> My apologies for the last message.  I am interested in locating
> kits for generating a genomic library (not cDNA).  Sorry for the confusion and many thanks to those who replied with help.
> 
> Fiona
> 
> 
> 
> This E-mail brought to you by Excite's free E-mail service.
> Get your own E-mail address at http://www.excite.co.uk
> 
> 

From owner-rapd@net.bio.net Thu Jan 21 22:00:00 1999
From: fixkdlno@domainserver01.com
Subject: Access to your online home business resources guide FREE
Newsgroups: bionet.molbio.rapd
NNTP-Posting-Host: klg-58-56.tm.net.my
X-NNTP-Posting-Host: klg-58-56.tm.net.my
Message-ID: <36a8e372.0@news2.tm.net.my>
Date: 23 Jan 1999 04:45:38 +0800
X-Trace: 23 Jan 1999 04:45:38 +0800, klg-58-56.tm.net.my
Organization: TMnet Malaysia
Lines: 26
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.berkeley.edu!newsfeed.cwix.com!202.188.95.15!news1.tm.net.my!klg-58-56.tm.net.my


This article is posted by Newsgroup AutoPoster PRO (unregistered)
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You can now access to your online home based business
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From owner-rapd@net.bio.net Tue Jan 26 22:00:00 1999
Path: biosci!MBCO.COM!Bower.Patricia
From: Bower.Patricia@MBCO.COM ("Bower, Patricia")
Newsgroups: bionet.molbio.rapd
Subject: Contract 2-hybrid
Date: 27 Jan 1999 13:17:21 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 4
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <755BF63198D8D1118CD80000F86311C9207F53@mbs00160.mbc.com>
NNTP-Posting-Host: net.bio.net

Does anyone know of any companies or universities that do contract 2-hybrid
screens.

Pat

From owner-rapd@net.bio.net Tue Jan 26 22:00:00 1999
Path: biosci!MAIL.ARGYRO.NET!vincent.w
From: vincent.w@MAIL.ARGYRO.NET
Newsgroups: bionet.molbio.rapd
Subject: GENOMICS AND THE NEW MOLECULAR TOOLS
Date: 27 Jan 1999 01:17:13 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 131
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   		  Last Announcement
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   7th AGROGENE INTERNATIONAL SEMINAR

 **GENOMICS AND THE NEW MOLECULAR TOOLS**

     February 25-26, 1999 - Paris - FRANCE

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New molecular tools coming from the genome analysis  (mapping,
sequencing, mutagenesis, DNA chips.) now allow the use of a large
amount of information.

AGROGENE 7th seminar is organised as a meeting place around talks
about genomics and new molecular tools of worldwide scientists :
   * Perspectives,
   * Techniques,
   * Bioinformatics,
   * Applications.

Besides the scientific talks, the schedule reserves a large place for
discussions. In parallele, an exhibition will present some of the
latest equipments and services. 

Thursday February 25th

Session  9h00 - 12h30
PERSPECTIVES:
Scott TINGEY - Dupont Agricultural Biotechnology - USA
Gene discovery for agricultural biotechnology.

Eric HUTTNER - Biogemma -France
Plant genomics, French perspectives: the Genoplante programme.

Break 

TECHNOLOGIES:
Marc MALANDRO - Perkin Elmer AgGen- USA
PE AgGen Genomics Program : the application of Perkin Elmer 
Technologies to Agricultural Gene Discovery.

Philippe REYMOND -University of Lausanne - Switzerland
Analysis of plant defense in Arabidopsis using cDNA microarrays. 

Lunch

Emmanuel MARTIN - Perkin Elmer Applied Biosystems - France
Real time PCR : a powerful tool for DNA/RNA quantitation.

Session 14h30 - 18h00
BIO-INFORMATICS:
Chris FIELD - PE Informatics - USA
Horizontal integration of processes and data.

Dave MATTHEWS - Cornell University - USA
Crop genome databases, a US perspective.

Jo DICKS - John Innes Center - UK
Bio-informatics for comparative genome analysis.

Dinner

Friday  February 26th
Session  9h00 - 12h30
APPLICATIONS:

Francis QUETIER - National center of sequencing - France
Sequencing activity at GENOSCOPE.

Graham PLASTOW - PIC Group - UK
First applications of molecular tools in pig breeding.

Break

Stephen J. OPENSHAW - Pioneer Hi-Bred Int. - USA
QTL detection and Marker Assisted Selection in commercial maize 
breeding.

Pierre ROUZE -Institut de Biotechnologie des Flandres - Belgium
From data on the Arabidopsis model genome to knowledge in plants. 

Lunch

Session  14h30 -18h00

Andreas GRANER - IPK Gatersleben - Germany
Barley breeding : from genetics to genomics.

Peter LANGRIDGE - University of Adelaïde -Austria
ITEC and cereal genomics.

Break

Greg PENNER - Monsanto -USA
Genomics in wheat.

Katrien DEVOS - John Innes Center - UK
Cereal genomes analysis;  the past, present and future.

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
CONTACT FOR REGISTRATION AND INFORMATION

AGROGENE
Vincent WICKAERT
620 rue Blaise Pascal - F-77555 Moissy Cramayel - FRANCE
Tel +33 1 64 13 31 80	Fax +33 1 64 13 31 81
http://www.seminar.agrogene.com
vincent.w@agrogene.com
__________________________________________________

REGISTRATION FEES

3 500 FF HT  for attendees from private companies
3 000 FF HT for attendees from public institutions 







______________________________________________________________
Vincent WICKAERT	       *            Marketing
AGROGENE S.A.		       *                &
620 rue Blaise Pascal            *    	   Development
F-77555 Moissy Cramayel        *
FRANCE			       *    vincent.w@agrogene.com
Tel +33 1 64 13 31 80		 	Fax +33 1 64 13 31 81
**************************************************************

