From owner-rapd@net.bio.net Tue Jun 01 02:28:00 1999 Path: biosci!DMSNA.DMS.UNINA.IT!noy2 From: noy2@DMSNA.DMS.UNINA.IT Newsgroups: bionet.molbio.rapd Subject: ADV: Photo Mousepads......These are cool! Date: 31 May 1999 20:28:39 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 125 Sender: daemon@net.bio.net Distribution: world Message-ID: <199905312818PAA8115@heyemailsavestrees.chemie.uni-wuerzburg.de> NNTP-Posting-Host: net.bio.net To be removed from our mailing list please call 800-242-0363 ext. 2759 This is a one time mailing and you will not receive another offer. Please click delete. GREAT GRADUATION GIFT IDEA! MOUSEPADS WITH YOUR FAVORITE PHOTOS! Need a new look for your desk? Need a new mousepad to go with your new computer? Now you can have your favorite photos printed onto mousepads! Photo mousepads are our most popular gift year after year! In the past we have provided our HIGH QUALITY mousepads to thousands of customers. 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BOX #782 ROSS, CA 94957 * Please allow 3 weeks for delivery. * Remember to include the proper shipping and handling fees ORDER TODAY ! = From owner-rapd@net.bio.net Wed Jun 02 13:28:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!dispose.news.demon.net!demon!newsfeed.nacamar.de!newsfeed.nacamar.de!fu-berlin.de!server1.netnews.ja.net!hgmp.mrc.ac.uk!biosci From: Wally_Dioh@monsantoseed.com Newsgroups: bionet.molbio.rapd Subject: RAPD primers web site Date: 2 Jun 1999 15:22:38 +0100 Organization: MRC Human Genome Mapping Project Resource Centre Message-ID: NNTP-Posting-Host: mercury.hgmp.mrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII; name="cc:Mail" Content-Transfer-Encoding: 7bit X-Trace: niobium.hgmp.mrc.ac.uk 928333358 27526 193.62.192.80 (2 Jun 1999 14:22:38 GMT) X-Complaints-To: news@hgmp.mrc.ac.uk NNTP-Posting-Date: 2 Jun 1999 14:22:38 GMT Content-Disposition: inline; filename="cc:Mail" X-Received: from mserv1.dl.ac.uk (root@mserv1.dl.ac.uk [148.79.160.65]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with ESMTP id PAA20791 for ; Wed, 2 Jun 1999 15:22:35 +0100 (BST) X-Received: from charon.cargill.com (charon.cargill.com [167.136.225.225]) by mserv1.dl.ac.uk with ESMTP id PAA27142 (8.8.8/5.4[ref postmaster@dl.ac.uk] for dl.ac.uk from Wally_Dioh@monsantoseed.com); Wed, 2 Jun 1999 15:22:31 +0100 (BST) X-Precedence: first-class X-Received: from hermes.cargill.com (hermes.cargill.com [167.136.226.140]) by charon.cargill.com (8.8.8/8.8.8) with ESMTP id JAA16362 for ; Wed, 2 Jun 1999 09:21:51 -0500 (CDT) X-Received: from peyr.cgo.cargill.com (peyr.cgo.cargill.com [172.29.40.51]) by hermes.cargill.com (8.8.8/8.8.8) with ESMTP id JAA24661 for ; Wed, 2 Jun 1999 09:23:27 -0500 (CDT) X-Received: from localhost (root@localhost) by peyr.cgo.cargill.com (AIX4.2/UCB 8.7/8.7) with SMTP id QAA08194 for rapd@dl.ac.uk; Wed, 2 Jun 1999 16:21:48 +0200 (DFT) X-OpenMail-Hops: 1 X-TO: rapd@dl.ac.uk Lines: 9 Dear all I am looking for a web site containing all RAPD OPERON sequences primers ? Thank you --- From owner-rapd@net.bio.net Wed Jun 02 14:00:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!logbridge.uoregon.edu!news.u.washington.edu!toby From: toby@u.washington.edu ('Toby' H D Bradshaw) Newsgroups: bionet.molbio.rapd Subject: Re: RAPD primers web site Date: 2 Jun 1999 14:48:24 GMT Organization: University of Washington, Seattle Lines: 9 Message-ID: <7j3g7o$mro$1@nntp4.u.washington.edu> References: NNTP-Posting-Host: homer30.u.washington.edu X-Trace: nntp4.u.washington.edu 928334904 23416 (None) 140.142.17.37 X-Complaints-To: help@cac.washington.edu NNTP-Posting-User: toby In article , wrote: >I am looking for a web site containing all RAPD OPERON sequences >primers ? http://dendrome.ucdavis.edu/Data/primer.html Toby Bradshaw | (206)616-1796 (voice) College of Forest Resources | (206)685-2692 (FAX) University of Washington | http://poplar2.cfr.washington.edu/toby From owner-rapd@net.bio.net Wed Jun 02 14:14:00 1999 Newsgroups: bionet.molbio.rapd Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!dispose.news.demon.net!demon!nntp.news.xara.net!xara.net!server6.netnews.ja.net!leeds.ac.uk!news From: BGYCSW@leeds.ac.uk (C.S. Wilding) Subject: Re: RAPD primers web site Message-ID: <7j3g73$87s_001@leeds.ac.uk> NNTP-Posting-Host: bgypc051.leeds.ac.uk Organization: University of Leeds Date: Wed, 2 Jun 1999 15:48:18 +0100 (BST) References: X-Newsreader: News Xpress Version 1.0 Beta #4 Lines: 17 Try: gopher://s27w007.pswfs.gov/1/.Data/.Primers/.RAPD or http://www.operon.com Craig. In article , Wally_Dioh@monsantoseed.com wrote: >Dear all > >I am looking for a web site containing all RAPD OPERON sequences >primers ? > >Thank you > > >--- From owner-rapd@net.bio.net Fri Jun 04 11:26:00 1999 Path: biosci!ALTERN.ORG!mhrk From: mhrk@ALTERN.ORG ("Hans") Newsgroups: bionet.molbio.rapd Subject: RAPD and Salmonella Date: 4 Jun 1999 05:26:34 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 60 Sender: daemon@net.bio.net Distribution: world Message-ID: <003001beae85$3db4e020$560eead4@bfk> NNTP-Posting-Host: net.bio.net This is a multi-part message in MIME format. ------=_NextPart_000_000F_01BEAEA5.F1B720E0 Content-Type: text/plain; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable I'm a student from the Faculty of Agriculture in Food and Agricultural = Biotechnology and i'm working on my final year, BSc project. I would need information on primers that can be used for this purpose. = I'm gonna work on the genetic diversity and virulence of salmonella spp = in my country. =20 Can you help?? =20 Thanks Hans =20 ------=_NextPart_000_000F_01BEAEA5.F1B720E0 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable
 
I'm a student = from the=20 Faculty of Agriculture in Food and Agricultural Biotechnology and i'm = working on=20 my final year, BSc project.
 
I would need information on primers that can be used = for this=20 purpose. I'm gonna work on the genetic diversity and virulence of = salmonella spp=20 in my country.
 
Can you help??
 
Thanks
Hans
 
------=_NextPart_000_000F_01BEAEA5.F1B720E0-- From owner-rapd@net.bio.net Mon Jun 07 17:31:00 1999 Path: biosci!gurlymail.com!asodfgri From: asodfgri@gurlymail.com Newsgroups: bionet.molbio.rapd Subject: $39.95/mo Real Time E-Commerce!!! Date: 7 Jun 1999 11:31:21 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 86 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hello: Our Company, specializes in providing "turn-Key" Solutions for your e-commerce needs. With nearly 70,000 clients and growing, we are proud to make the following limited time offers. We have temporarily slashed our prices to encourage your business! We assume you understand the value of being able to accept credit card payments from your customers, and are offering three solutions to fit your needs. Choose Either System For $39.95/mo!!! Professional Version: -- Full Blown online real time processing system. 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Sincerely, Total Merchant Services 1-800-242-0363 x 1732 ===================================== For list removal, please type REMOVE in the subject field and send to nomail01@quakemail.com From owner-rapd@net.bio.net Tue Jun 08 05:45:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.berkeley.edu!intgwpad.nntp.telstra.net!news1.optus.net.au!optus!bunyip.cc.uq.edu.au!axis.scu.edu.au!ip3956.scu.edu.au!user From: rkota@scu.edu.au (Raja Kota) Newsgroups: bionet.molbio.rapd Subject: AFLP bands Date: Tue, 08 Jun 1999 16:36:48 +1000 Organization: Southern Cross University Lines: 15 Message-ID: NNTP-Posting-Host: ip3956.scu.edu.au Hi Netters, I have been cloning some AFLP bands from polyacrylamide gel. Once I excise the band from the gel, I resuspend it in sterile water (50µl) o/n at 37C. Following this I use part of this as my template and re-amplify the fragment. However, every time I re-amplify the fragment, I do not get the expected size. Intrestingly all the bands are approximately half the size of the original band. For instance, if I had excised a 500 bp fragment, I seem to re-amplify a 250 bp fragment. I am bewildered by this observation and am looking at some way to explain this phenomenon. If you have any suggestions, please e-mail me directly on rkota@scu.edu.au Thanks in advance, Raja From owner-rapd@net.bio.net Wed Jun 09 06:45:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!nntp.cs.ubc.ca!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!news.algonet.se!algonet!news-peer-europe.sprintlink.net!news.sprintlink.net!newsfeed.sunet.se!news01.sunet.se!news99.sunet.se!news.datakom.su.se!Jorgen.Ullberg From: Jorgen.Ullberg@zoologi.su.se (=?ISO-8859-1?Q?J=F6rgen_Ullberg?=) Newsgroups: bionet.molbio.rapd Subject: Re: RAPD and Salmonella Date: Wed, 9 Jun 1999 09:44:46 +0200 Organization: Stockholm University Lines: 19 Message-ID: <1dt2r5g.r5jc6m8hw89kN@ullberg.zoologi.su.se> References: <003001beae85$3db4e020$560eead4@bfk> NNTP-Posting-Host: ullberg.zoologi.su.se User-Agent: MacSOUP/2.4 "Hans" wrote: > > I'm a student from the Faculty of Agriculture in Food and Agricultural = > Biotechnology and i'm working on my final year, BSc project. > > > I would need information on primers that can be used for this purpose. = > I'm gonna work on the genetic diversity and virulence of salmonella spp = > in my country. > Try the following URL to Operons RAPD-primer kit site, where they have plenty of ready made kits. Each primer are 10 bp and there are 20 primers per kit. http://web712d0.ntx.net/ss2b1/stockproducts/10merinfo.html -- Jorgen From owner-rapd@net.bio.net Fri Jun 11 00:09:00 1999 Path: biosci!SCIENCEWEEK.COM!prismx From: prismx@SCIENCEWEEK.COM ("Science-Week") Newsgroups: bionet.molbio.rapd Subject: ScienceWeek Contents Date: 10 Jun 1999 18:09:03 -0700 Organization: Science-Week Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <199906110059.RAA27213@wren.prod.itd.earthlink.net> Reply-To: prismx@scienceweek.com NNTP-Posting-Host: net.bio.net This week in ScienceWeek: June 11, 1999 -- Vol. 3 Number 24 ----------------------------------------------- 1. Academia vs. Industry: A US$1 Billion Hormone Patent Battle 2. Cosmology: On the Epoch of Galaxy Formation 3. Earth Science: On Mantle Plumes and Mountains 4. On the Evolutionary Tree of Life 5. Neurobiology: Dendrite Morphology Induced by Synaptic Activity 6. Medical Science: Deaths Related to Liposuction In Focus: On the Evolution of the Vertebrate Brain The complete text of report Number 6 is posted at the SW website URL http://www.scienceweek.com/ [If you have not yet requested it, and you would like to receive this notice each week via Email, simply reply to this message with SEND CONTENTS as subject.] Claire Haller Managing Editor ScienceWeek haller@scienceweek.com http://www.scienceweek.com From owner-rapd@net.bio.net Mon Jun 14 02:34:00 1999 Path: biosci!INAME.COM!lanes254 From: lanes254@INAME.COM Newsgroups: bionet.molbio.rapd Subject: You're Approved!-Accept Credit Card Date: 13 Jun 1999 20:34:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 40 Sender: daemon@net.bio.net Distribution: world Message-ID: <199906140424.FAA05766@ilsol01> NNTP-Posting-Host: net.bio.net Thank you for your Free For All Link posting and feel free to post again. 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To be Removed from our list hit the reply button and send email with "Remove" in the Subject: From owner-rapd@net.bio.net Mon Jun 14 15:06:00 1999 Path: biosci!WSUNIX.WSU.EDU!arthurr From: arthurr@WSUNIX.WSU.EDU (arthur roberts) Newsgroups: bionet.molbio.rapd Subject: Information in molecular Biology Date: 14 Jun 1999 09:06:16 -0700 Organization: Institute of Biological Chemistry Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <3765266E.F43EF346@wsunix.wsu.edu> NNTP-Posting-Host: net.bio.net Art's Biotechnology Resource ---------------------------- The website can be linked by the following: http://biotech.isCool.net or http://www.ahpcc.unm.edu/~aroberts or http://www.arc.unm.edu/~aroberts This site allows easy access to hyperlinks and free software for the biochemist, biophysicist and molecular biologist. This site is constantly evolving and expanding, so it can be easy to use, reliable, and comprehensive. As of 6/13/99, many new links have been added. There have also been some new categories added: Employment Opportunities: this is a list of the "searchable" databases with jobs that are related to the biotechnology field. If you know of any links that are related to employment, please email me at arthurr@wsunix.wsu.edu Best Biotechnology Sites: this is a list of the best biotechnology- related sites in the world. If you know of any websites that are not on the list that would be considered the best, please email me at arthurr@wsunix.wsu.edu Bioethics/ Legal Issues: provides information on the public's view of biotechnology research and legal issues that concern the biotechnology community. If you know of any other important sites related to the legality of biotechnology or ethics (i.e. human cloning), please email me at arthurr@wsunix.wsu Sincerely, Art Roberts (web designer) arthurr@wsunix.wsu.edu (P.S. I reply to all emails that are sent, and I appreciate your comments and suggestions) From owner-rapd@net.bio.net Thu Jun 17 08:00:00 1999 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Jun 1999 02:00:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199906170900.CAA13692@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. 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