From owner-rapd@net.bio.net Fri Jul 02 19:21:00 1999 Path: biosci!ROCKETMAIL.COM!albulb From: albulb@ROCKETMAIL.COM (alberto donayre) Newsgroups: bionet.molbio.rapd Subject: Dominant markers Date: 2 Jul 1999 13:21:50 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <19990702201450.6393.rocketmail@web4.rocketmail.com> NNTP-Posting-Host: net.bio.net Hi All! I am from Perú working in plant biodiversity of native Andean Roots using RAPD molecular markers. As far I know RAPDs are in the group of the dominant markers but,what exactly does it mean? how do they recognize dominant alleles in the entire genome? . I will appreciate your assistance in sending me some ideas and/or references. Thanks in advance, Alberto Donayre Universidad San Marcos www.unmsm.edu.pe/biologia Facultad de Biologia Lima-Perú *********************** _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-rapd@net.bio.net Wed Jul 14 11:52:00 1999 Path: biosci!news.stanford.edu!newsfeed.stanford.edu!newsfeed.concentric.net!newspeer.monmouth.com!news.algonet.se!newsfeed1.telenordia.se!algonet!newsfeed1.funet.fi!newsfeed.sunet.se!news01.sunet.se!news99.sunet.se!news.su.se!Jorgen.Ullberg From: Jorgen.Ullberg@zoologi.su.se (=?ISO-8859-1?Q?J=F6rgen_Ullberg?=) Newsgroups: bionet.molbio.rapd Subject: Re: Dominant markers Date: Wed, 14 Jul 1999 14:56:52 +0200 Organization: Stockholm University Lines: 40 Message-ID: <1duvqwi.vjjp1413cw9n8N@ullberg.zoologi.su.se> References: <19990702201450.6393.rocketmail@web4.rocketmail.com> NNTP-Posting-Host: ullberg.zoologi.su.se Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Trace: news.su.se 931952715 12022 130.237.191.183 (14 Jul 1999 11:45:15 GMT) X-Complaints-To: abuse@su.se NNTP-Posting-Date: 14 Jul 1999 11:45:15 GMT User-Agent: MacSOUP/2.4 alberto donayre wrote: > I am from Perú working in plant biodiversity of > native Andean Roots using RAPD molecular markers. As > far I know RAPDs are in the group of the dominant > markers but,what exactly does it mean? how do they > recognize dominant alleles in the entire genome? . I > will appreciate your assistance in sending me some > ideas and/or references. > Hi Alberto! In short, the RAPD method is not able to distinguish between dominant vs. recessive alleles at a locus. Such as AA, Aa or aa or how many alleles there are at that locus. I was told however, that in general the loci when doing RAPD are considered bi-allelic. In that wiev the markers are regarded as dominant. Below are some references covering this aspect and a few other useful topics in RAPD. Lynch M., Milligan B. (1994) Analysis of population genetic structure with RAPD markers. Molecular Ecology 3(2): 91-99 Black W. (1993) PCR with arbitrary primers: approach with care. Insect Molecular Biology 2(1): 1-6 Dean R., Arnold J. (1996) Cytonuclear disequilibria in hybrid zones using RAPD markers. Evolution 50(4): 1702-1705 Williams J., Hanafey M., Rafalski J., Tingey S. (1993) Genetic Analysis Using Random Amplified Polymorphic DNA Markers. Methods in Enzymology 218:704-740 Tingey S., Tufo J. d. (1993) Genetic analysis with random amplified polymorphic DNA markers. Plant Physiology 101(2): 349-352 Good luck Jorgen From owner-rapd@net.bio.net Fri Jul 16 15:16:00 1999 Path: biosci!ROSA.ESB.UCP.PT!qooli7 From: qooli7@ROSA.ESB.UCP.PT (ytukay2) Newsgroups: bionet.molbio.rapd Subject: ADV: Is the Government covering up the "Y2K" crisis? Date: 16 Jul 1999 09:16:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 80 Sender: daemon@net.bio.net Distribution: world Message-ID: <199907161711CAA54130@kloeswez.ee.sophia.ac.jp> NNTP-Posting-Host: net.bio.net To be removed, just email tvmoto@bigfoot.com -*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-* Every Citizen deserves to know the truth and the facts about the Y2K computer problem. Many governmental officials have spoken to the public on the topic of Y2K for the purpose of not creating any panic to the communities. Unfortunately, this governmental approach on the problem is provoking a passive attitude and creating a false sense of security within Citizens with respect to this extremely vital issue! Do not be deceived! How much do you really know about the year 2000 computer crisis? Take a look at the following statements made by the U.S. Senator Robert F. Bennett (R-Utah), Chairman of the Senate Special Committee on the Year 2000 Technology Problem: "The financial services industry is particularly susceptible to the Year 2000 problem. Without reliable systems... interest could be miscalculated, stock trades could vanish, and customers could have difficulty accessing their account balances or their credit cards." The Year 2000 problem -- or the inability of computers to recognize dates beyond 1999 -- could potentially cause national and worldwide computer systems to shut down or make major mistakes at the beginning of the next century". "While I do hope the FAA is doing as well as it says, experience has taught me to be suspicious of easy answers and quick fixes," he added. "One example is the recent overstatement by the Department of Defense, exposed when its Inspector General reported that 70 percent of the mission critical systems the department deemed to be Y2K compliant were, in fact, not." Take a look at the following statement relating to the business community: "Businesses that fail to properly correct their systems could be committing corporate suicide," Bennett said. "The biggest Y2K headache of all could be the legal fees and lawsuits that cripple America's employers and working people long after the Y2K bug has been chased from computer hardware and software." As a consumer or a business owner you have the right to obtain quality information on how to confront the year 2000 computer crisis successfully. Do you have precise answers to the following questions? Is your personal computer or network going to survive the coming crash? Are your software and data files going to be DESTROYED by the "Millennium Bug"? Is your computer system fully YEAR 2000 COMPLIANT, even if it is new? Do you know how the "Electronic Data Interfacing" could severely harm consumers and businesses? Your business survival will depend on you obtaining quality information and FACTS. Learn how to protect your business against individuals and lawyers who may be currently preparing to file lawsuits against your business for not being fully Y2K compliant. Denying or ignoring the problem would be a foolish position to take. Learn why your computer compliance efforts should meet the internationally adopted standard BSI (British Standard Institution for Year 2000 Compliance) Learn more about the most recent technical Y2K problem found in personal computers and networks-- the "Crouch-Echlin Time Dilation Effect". Ignoring this problem can send your compliance efforts down the drain. How would you like to make $ thousands of dollars by taking advantage of the income opportunities originated by the year 2000 computer crisis. Obtain a FREE report on the facts and solutions to the upcoming Y2K computer crash. click here for your FREE report --Thank-You: for removal see above instructions ùsending m From owner-rapd@net.bio.net Sat Jul 17 08:00:00 1999 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Jul 1999 02:00:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199907170900.CAA29414@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-rapd@net.bio.net Mon Jul 19 12:44:00 1999 Path: biosci!internet!biosci!not-for-mail From: daemon@net.bio.net Newsgroups: bionet.molbio.rapd Subject: Fwd: Date: 19 Jul 1999 06:44:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 46 Distribution: world Message-ID: <74210.35259@zebra.ini.unizh.ch> NNTP-Posting-Host: net.bio.net Adult Webhosting starting at only $39.95 per month. Call for details 407-599-5253 Complete Turn Key Adult Website only $3500.00. You will not find an easier or more complete way to enter the lucrative online adult industry. We design it, program it, provide all the content, set up the merchant account and billing system, and help you market it. As if that's not enough, your site content is updated weekly at no charge to keep your customers coming back month after month. Call 407-599-5253 The US Adult Industry at a glance: 1998 Revenues: $9 Billion 1999 Revenues $12 Billion (Projected) June Special: Sign up now and get an additional 3 months of hosting.(6 month total) Your Complete Adult Website Includes: - Your own domain name (www.yourdomainname.com,.we pay the InterNIC Fees) - 3 months web hosting (3 mo. bonus through June 30, for a total of 6months) - Adult Online Store (Make 20% of sales through your site) - 1000s of pics with over 90 themes updated weekly from database of over200,000 - Your own state-of-the-art credit card processing and customer billingsystem - 5000+ Adult Video Channels - 30+ Live Chat Channels (Video Included) - Celebrity Nude Section- XXX Hardcore Channels (Over 5000 Shows) - Your own video store with 2000 titles for free download (AVI, MPEG, and QuickTime) - Erotic Stories (Text and Real Audio Format) - Strip Club Database (Listings by State, City, and Region) - Adult Classified Section - 100+ Web Cams - 10 Live Streaming Feeds - 100+ Sex Games - Jokes Area And much more...... We have created over 140 turnkey sites to date with an average monthly subscriber price of $19.95. We help you get subscribers, and the massive content in your site keeps them there. Once they subscribe, your advanced merchant system bills them every month until they cancel. It assigns and takes away passwords and eliminates fraud. We invite you to look around and compare. There is no better system out there. Call us at 407-599-5253 Call 407-599-5253 ********************************************************** If you do not want to receive any further messages from us, please call 1 (800) 409-8312 and spell your email address if necessary. Thank You ********************************************************************* From owner-rapd@net.bio.net Thu Jul 22 06:14:00 1999 Path: biosci!CASSANDRA.SGGW.WAW.PL!regebao15 From: regebao15@CASSANDRA.SGGW.WAW.PL (yew4ose) Newsgroups: bionet.molbio.rapd Subject: F-R-E-E--> Weight.Loss.Gift Date: 22 Jul 1999 00:14:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <199907224008NAA36145@hugyytehn.com.tw> NNTP-Posting-Host: net.bio.net Greetings from Roy Hinman II M.D., I am a Family Practice physician and Weight Loss Specialist. My case studies of 1500 patients has consistantly proven to produce dependable and prompt results of a minimum 1/2 lb. to 1 lb. of body fat loss per day. That is 15 to 30 lbs. of assured body fat loss every 30 days. The program is "Physician's Weight Management". It is simple, safe, all natural, easy to use and is absolutely 100% money back guaranteed. Please reply by e-mail for detailed information and you will receive "The Doctor's 30 Answers To Weight Loss" for free, without obligation. For info: email rightfoot1@bigfoot.com with subject weightloss Removes : email rightfoot1@bigfoot.com with subject remove ** Please follow instructions for info or removal...thanks thanks and have an nice day a/ggw.waw.