From owner-rapd@net.bio.net Wed Sep 01 01:46:00 1999 Path: biosci!ICOM.ICOM.IT!qeeriotu44 From: qeeriotu44@ICOM.ICOM.IT (covmserh) Newsgroups: bionet.molbio.rapd Subject: Tibetan mushroom increases lung capacity and energy. Date: 31 Aug 1999 19:46:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <199908312366TAA4388@leuisoe.ser.co.kr> NNTP-Posting-Host: net.bio.net Athletes, work-out devotees and cholesterol-compromised people take note: The active ingredient of a rare mushroom that grows at 14,000 feet in Tibet and was reserved only for Chinese royalty has been synthesized. Cordyceps sinensis has been clinically tested to improve lung capacity and increase stamina and energy. In most cases it will also increase the HDL (good) cholesterol level. No side effects. Not sold in stores. $24.95 for month's supply. Also, we have standardized green tea capsules equivalent to four cups of green tea. One hundred times more potent an antioxidant than vitamin C, green tea polyphenols are the only antioxidants that penetrate the cell membrane and protect the DNA. Studies show they kill prostate cancer cells (Mayo Clinic) and prevent or alleviate rheumatoid arthritis ( Proceedings of the National Academy of Sciences). Our product has 97% total polyphenols-more than any other product in the industry, by far. Only $16.95 for month's supply. For info on how to buy or sell: email north34@bigfoot.com with the subject "energy" or send a fax to : 603-537-0753 To be deleted from the list, email north34@bigfoot.com with the subject line as "remove" Have a nice day OVER-- From owner-rapd@net.bio.net Wed Sep 01 04:20:00 1999 Path: biosci!WSUNIX.WSU.EDU!arthurr From: arthurr@WSUNIX.WSU.EDU (art roberts) Newsgroups: bionet.molbio.rapd Subject: Site with many molecular biology and genetics links!!! Date: 31 Aug 1999 22:20:19 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: <37CCB670.7C9031EF@wsunix.wsu.edu> NNTP-Posting-Host: net.bio.net Great, innovative, and expanding site found at http://www.biotech-resource.com Features -------- Bioethics/ Legal Issues - What are the ethical and legal issues in biotechnology today? Best sites in Biotechnology - It is a list of the Best Biotechnology Sites in the World. Employment opportunities - Employment opportunities in the Biotechnology/Biology field. Search Engine - this simple search engine allows access to all links and the information in the site. Add-A-Link - add your favorite link to my website. Book Reviews - it is a small list of books that I thought would be of a general interest. This site provides a wide range of information and links for the biochemist, biophysicist, molecular biologist, and science educator. There is also an extensive list of software resources that are free and available over the internet. This site is non-commercial and it is available for your enjoyment. I appreciate your comments and suggestions, and I will reply to all emails (email: arthurr@wsunix.wsu.edu). Coming Soon ----------- I also plan to have stock information available about biotechnology companies in the near future. Sincerely, Art Roberts (web designer) (P.S. This site can also be accessed by http://biotech.iscool.net or http://www.ahpcc.unm.edu/~aroberts , if you have trouble accessing it by http://www.biotech-resource.com .) From owner-rapd@net.bio.net Thu Sep 02 08:58:00 1999 Path: biosci!rz.uni-jena.de!Johannes.Woestemeyer From: Johannes.Woestemeyer@rz.uni-jena.de (Johannes Woestemeyer) Newsgroups: bionet.molbio.rapd Subject: Fungal Phylogeny for PhD students Date: 2 Sep 1999 02:57:55 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 62 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.3.32.19990902114941.006c10c4@pop3.uni-jena.de> NNTP-Posting-Host: net.bio.net Dear colleagues! Please forward this job offer to all those in your vicinity who could be interested in working on molecular phylogeny of fungi: In a project financed by 'Deutsche Forschungsgemeinschaft' we work on family and order structure of zygomycetes. Based on sequences of protein coding genes from the cellular functions 'cell cycle control', 'cytoskeleton' and 'translational apparatus' we want to add the phylogenetic dimension to a wealth of taxonomic knowledge and expertise in this important fungal group. The position is to be filled beginning of October at the earliest and end of the Winter term at the latest. Knowledge of German language is not required. Experimental working conditions are good. We are technically well equipped and have sound expertise at all levels of cloning and sequencing. Primers for the required genes have been developed and checked in screening experiments. Research is institutionally linked to an independent group at the institute of General Microbiology and Microbial Genetics, the 'Fungal Reference Centre' (FRC). The PhD candidate will be supervised by the FRC leader, Dr. Kerstin Voigt and by myself in close cooperation. Both supervisors have their experimental skills in this and other fields and perform experiments themselves within the project. We expect a highly motivated young colleague, integration into a vivid multidisciplinary institute, some expertise in Genetics and Molecular Biology as well as a feeling for evolutionary biology. Special taxonomic knowledge of fungi is not requested at the beginning. If interested, please send your details to Dr. Voigt or to me: Friedrich-Schiller-Universitaet Jena Lehrstuhl fuer Allgemeine Mikrobiologie und Mikrobengenetik Neugasse 24 D-07743 Jena Email applications are also possible: please direct to b5wojo@rz.uni-jena.de I will call back for further details or in order to make appointments for a personal date, ideally beginning of October. Best wishes, Johannes Woestemeyer ------------------------------------------------------- Prof. Dr. Johannes Woestemeyer Institute of General Microbiology and Microbe Genetics Friedrich-Schiller-University Jena Neugasse 24 D-07743 Jena - Germany Tel. : +49 (0)3641 949310/1 Fax : +49 (0)3641 949312 E-Mail : b5wojo@rz.uni-jena.de Homepage: http://www.uni-jena.de/biologie/mikrobio/ -------------------------------------------------------- From owner-rapd@net.bio.net Thu Sep 02 09:03:00 1999 Path: biosci!rz.uni-jena.de!Johannes.Woestemeyer From: Johannes.Woestemeyer@rz.uni-jena.de (Johannes Woestemeyer) Newsgroups: bionet.molbio.rapd Subject: Doktorandenstelle: Phylogenie der Pilze Date: 2 Sep 1999 03:03:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 66 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.3.32.19990902115456.006c10c4@pop3.uni-jena.de> NNTP-Posting-Host: net.bio.net Liebe Kolleginnen und Kollegen sowie alle Interessentinnen an einer Arbeit im Bereich Molekulare Phylogenie! Mit der Bitte um Weitergabe an Interessierte schicke ich Ihnen die folgende Ausschreibung: Die DFG foerdert uns ein Projekt, in dem es um die Neubewertung der Familien- und Ordnungsstruktur in einer grossen Gruppe von Pilzen (Zygomyceten) geht. Anhand der Sequenzen Protein-kodierender Gene aus 'Zellzyklus-Steuerung', 'Translationsapparat' und 'Cytoskelett' soll endlich das nicht geringe taxonomische Wissen ueber diese Pilze in einen phylogenetischen Kontext gebracht werden. Wir wollen die Stelle fruehestens zum 1. Oktober und (aller)spaetestens zum Ende des Wintersemesters besetzen. Bezahlung wie ueblich nach BAT IIa/2. Die experimentellen Voraussetzungen sind gut, da wir fuer alle benoetigten Gene passende Primerpaare erprobt haben. Apparativ sind wir im Hinblick auf PCR und Sequenzierung gut ausgestattet. Experimentell-technisches know-how ist bei allen Mitarbeitern im Haus vorhanden. Die Arbeit ist angesiedelt an einer selbstaendigen Abteilung am Institut, dem 'Pilz-Referenz-Zentrum Jena' (PRZ), das auf der Forschungsseite den Schwerpunkt auf der Molekularen Phylogenie der Pilze hat. Die Arbeit wird in enger Abstimmung von mir und Dr. Kerstin Voigt als Leiterin des PRZ betreut. Beide Betreuer/innen experimentieren auch selbst innerhalb des Projektrahmens. Wir erwarten von der Neuen oder dem Neuen hohe Motivation, Integration in ein reges, wissenschaftlich vielschichtiges Institut, Vorkenntnisse in Genetik und Molekularbiologie, sowie ein Feeling fuer Evolutionsbiologie. Spezielle taxonomische Kenntnisse sind erst einmal nicht erforderlich. Wenn Sie Interesse haben, bitte ich um Ihre Bewebungsunterlagen an Dr. Voigt oder mich unter der Adresse: Friedrich-Schiller-Universitaet Jena Lehrstuhl fuer Allgemeine Mikrobiologie und Mikrobengenetik Neugasse 24 D-07743 Jena Email-Berwerbungen an b5wojo@rz.uni-jena.de sind ebenfalls moeglich. Ich melde mich dann bei Ihnen wegen weiterer Auskuenfte oder eines Gespraechstermins, der im Idealfall Anfang Oktober stattfinden sollte, bei Ihnen zurueck. Mit freundlichem Gruss, Joh. Woestemeyer ------------------------------------------------------- Prof. Dr. Johannes Woestemeyer Institute of General Microbiology and Microbe Genetics Friedrich-Schiller-University Jena Neugasse 24 D-07743 Jena - Germany Tel. : +49 (0)3641 949310/1 Fax : +49 (0)3641 949312 E-Mail : b5wojo@rz.uni-jena.de Homepage: http://www.uni-jena.de/biologie/mikrobio/ -------------------------------------------------------- From owner-rapd@net.bio.net Thu Sep 02 11:00:00 1999 Path: biosci!rz.uni-jena.de!Johannes.Woestemeyer From: Johannes.Woestemeyer@rz.uni-jena.de (Johannes Woestemeyer) Newsgroups: bionet.molbio.rapd Subject: Assi-Stelle in Jena: Cell cycle Date: 2 Sep 1999 05:00:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 58 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.3.32.19990902135153.006d0c18@pop3.uni-jena.de> NNTP-Posting-Host: net.bio.net Liebe Kolleginnen, ich bitte um Bekanntgabe der folgenden Stellenausschreibung (auch erschienen in 'Die Zeit' vom 19. August) in Ihrem Umfeld. Mit herzlichem Dank, Johannes Woestemeyer Friedrich-Schiller-Universitaet Jena Zum naechstmoeglichen Termin, fruehestens zum 1.10.1999, ist die Stelle eines/einer Wissenschaftlichen Mitarbeiters/-in am Lehrstuhl fuer Allgemeine Mikrobiologie und Mikrobengenetik zu besetzen. Schwerpunktaufgaben: Uebernahme mikrobiologischer Grundkurse fuer den Studiengang Diplom-Biologie; Beteiligung am Grosspraktikum im Hauptstudium mit Schwerpunkten im Bereich Molekularbiologie und Genetik von pilzen. Forschungsthema ist die Klonierung und Charakterisierung von Zellcyclus-Genen endogener Mykorrhiza-Pilze. Die Einbindung in das Schwerpunktprogramm 'Mykorrhiza' der DFG wird angestrebt. Qualifikation: Promotion in Biologie oder Chemie/Biochemie Gewuenschte Expertise: Interesse fuer Pilz/Pflanze-Interaktionen; PCR- und Klonierungskenntnisse. Verguetung gemaess BAT-O/IIa; die Stelle ist zunaechst fuer zwei Jahre befristet. Bewerbungen von Frauen werden ausdruecklich begruesst. Schwerbehinderte werden bei gleicher Eignung bevorzugt beruecksichtigt. Bewerbungen mit vollstaendigen Bewerbungsunterlagen unter Angabe der Reg-No. 88/99 richten Sie bitte an: FSU Jena Lehrstuhl fuer Allg. Mikrobiologie und Mikrobengenetik Prof. J. Woestemeyer Neugasse 24 D-07743 Jena ------------------------------------------------------- Prof. Dr. Johannes Woestemeyer Institute of General Microbiology and Microbe Genetics Friedrich-Schiller-University Jena Neugasse 24 D-07743 Jena - Germany Tel. : +49 (0)3641 949310/1 Fax : +49 (0)3641 949312 E-Mail : b5wojo@rz.uni-jena.de Homepage: http://www.uni-jena.de/biologie/mikrobio/ -------------------------------------------------------- From owner-rapd@net.bio.net Thu Sep 02 11:10:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!newsfeed.icl.net!newsfeed.icl.net!newscore.gigabell.net!newscore.ipf.de!news-fra1.dfn.de!news.man.poznan.pl!news.task.gda.pl!orion.cst.tpsa.pl!news.tpnet.pl!not-for-mail From: "michael" Newsgroups: bionet.molbio.rapd Subject: DNA polymerases wanted Lines: 21 X-Newsreader: Microsoft Outlook Express 4.72.3110.1 X-MimeOLE: Produced By Microsoft MimeOLE V4.72.3110.3 Message-ID: Date: Thu, 02 Sep 1999 12:05:08 GMT NNTP-Posting-Host: 212.160.46.95 X-Complaints-To: abuse@tpsa.pl X-Trace: news.tpnet.pl 936273908 212.160.46.95 (Thu, 02 Sep 1999 14:05:08 MET DST) NNTP-Posting-Date: Thu, 02 Sep 1999 14:05:08 MET DST Organization: TPNET - http://www.tpnet.pl Hello, I'm looking for polymerases, in particular, would you know of or have a source for the following enzymes: 1. 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DISCLAIMER: The seller of this powerful software resource will not be held responsible for how the purchaser chooses to use its resources. ----------------------------------------------------------------------------------------------------------------- To UNSUBSCRIBE and be removed from this mailing, please leave us your complete email address on our toll-free voicemail server: 1-800-595-2001 HP400 From owner-rapd@net.bio.net Mon Sep 06 14:49:00 1999 Path: biosci!163.NET!jiayun98 From: jiayun98@163.NET Newsgroups: bionet.molbio.rapd Subject: Need E.coli J5 Date: 6 Sep 1999 08:49:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <19990906151813.17675.fmail@163.net> Reply-To: jiayun98@163.net NNTP-Posting-Host: net.bio.net Dear colleagues! I'm a medical student of Xijing Hospital. I need Escherichia coli J5 strain urgently in my experiments,unfortunately it is not available from our resources. Could anyone give me some Escherichia coli J5 strain? Any help would be greatly appreciated! My email: jiayun98@163.net Address: Department of Clinical Laboratory, Xijing Hospital, 710032 Xi'an, China. Jiayun Liu 1999.09.06 ________________________________________________ »¶Ó­ÄúʹÓÃ163µç×ÓÓʾÖÃâ·Ñ·þÎñ http://www.163.net 163³¬¼¶¿áÈ«¹ú×î´óµÄÃâ·Ñ¸öÈËÖ÷Ò³»ùµØhttp://cool.163.net From owner-rapd@net.bio.net Mon Sep 06 14:49:00 1999 Path: biosci!163.NET!jiayun98 From: jiayun98@163.NET Newsgroups: bionet.molbio.rapd Subject: Need E.coli J5 Date: 6 Sep 1999 08:49:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: <19990906151756.17549.fmail@163.net> Reply-To: jiayun98@163.net NNTP-Posting-Host: net.bio.net Dear colleagues! I'm a medical student of Xijing Hospital. I need Escherichia coli J5 strain urgently in my experiments,unfortunately it is not available from our resources. Could anyone give me some Escherichia coli J5 strain? Any help would be greatly appreciated! My email: jiayun98@163.net Address: Department of Clinical Laboratory, Xijing Hospital, 710032 Xi'an, China. Jiayun Liu 1999.09.06 ________________________________________________ »¶Ó­ÄúʹÓÃ163µç×ÓÓʾÖÃâ·Ñ·þÎñ http://www.163.net 163³¬¼¶¿áÈ«¹ú×î´óµÄÃâ·Ñ¸öÈËÖ÷Ò³»ùµØhttp://cool.163.net From owner-rapd@net.bio.net Tue Sep 07 01:12:00 1999 Path: biosci!GNWMAIL.COM!cybermarket From: cybermarket@GNWMAIL.COM Newsgroups: bionet.molbio.rapd Subject: Home loans even if you have bad credit! 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Refinancing - Reduce your monthly payments and get cash back! From owner-rapd@net.bio.net Thu Sep 09 01:12:00 1999 Path: biosci!TUSK.EDU!prakash From: prakash@TUSK.EDU ("C. S. Prakash") Newsgroups: bionet.molbio.rapd Subject: POSITION: ICRISAT: HEAD, APPLIED GENOMICS LABORATORY Date: 8 Sep 1999 19:11:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 71 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net POSITION: ICRISAT - HEAD, APPLIED GENOMICS LABORATORY [CODE : IC-02/99- C] The International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) is a nonprofit, autonomous, scientific research and training institute receiving support from donors through the Consultative Group for International Agricultural Research (CGIAR). ICRISAT undertakes global research into the improvement of six crops (sorghum, pearl millet, finger millet, groundnut, chickpea and pigeonpea) and into resource management technologies to alleviate rural poverty in the semi-arid tropics which is home to one-sixth of the world's population. ICRISAT needs a Head, Applied Genomics Laboratory (AGL) to be based at Patancheru, Andhra Pradesh, India, who will provide technical leadership and innovation in indentification, testing and implementation of molecular strategies, techniques and analyses to enhance the conservation and utilization of crop germplasm and investigage pathogens. The Head, (AGL) reports to the Program Director- Genetic Resources and Enhancement Program (GREP). The appointee will a) support curatorial needs for the Gene Bank by describing genomic variation and monitor changes in allele frequencies; b) strengthen genetic enhancement via gene mapping and marker identification for assisted-selection and aided-introgression using both the wild relatives of crop plants as well as landrace germplasm. The appointee's specific duties include a) developing linkages and collaborative research projects with advanced research organizations, national agricultural research systems and universities in the semi-arid tropics; b) co-ordinate grant proposals jointly with other research partners and submit them for funding; d) coordinate the operations of laboratory to perform automated DNA sequencing and fragment analysis, automated DNA synthesis and DNA amplification; e) coordinate DNA and RNA extraction, genomic and cDNA library construction, data analysis, dissemination and documentation, presentation of results in scientific journals and at meetings; and f) train graduate students and research fellows and supervise laboratory technical staff and students. Applicants should have a PhD degree in Plant Biology, Plant Breeding, Genetics or any other related field with at least 5 years of post-doctoral experience in molecular genetics, molecular biology or molecular breeding, including international exposure in genetic resources or genetic enhancement research and development. A record of achievement with academic publications, successful grants management and leadership abilities is essential. Experience in effective operation of a genetic analysis laboratory, and ability to work well with staff, visiting scientists and students are prerequisites. International experience in co-ordinating/conducting research, education or development programs is desirable. Fluency in spoken and written English is essential and knowledge of French is desirable. Sensitivity in working in a multicultural environment will be given priority. ICRISAT is an equal opportunity employer and strives for staff diversity in gender and nationality. ICRISAT Headquarters is located at Patancheru near Hyderabad, India with regional centers and teams in Asia and Africa. The Center's salaries and benefits are competitive with those of other international institutions. Further information about ICRISAT and the CGIAR can be obtained via the internet at NNTP-Posting-Host: net.bio.net ELECTRONIC MARKETING Cyberpreneurs are increasing tremendously with the boom of the digital/electronic frontier. Electronic marketing is a proven method with a global market. It is more advantageous than conventional methods and the risk is minimal. The prospects are millions. SUCCESSFUL ITEMS FOUND IN CYBERSPACE The existence of the Information Superhighway narrows down to one important factor; information. It's been proven repeatedly that the ideal cyberspace products are are books, software, computer related materials, information, and services. PRODUCTION COSTS Regardless of the product/service, most experts agree you must be able to produce the product to keep your product acquisition costs DOWN. Because of the digital era and the medium created by the internet, many products can now be produced in-house, such as software, information, electronic books, internet services, etc... AN UNMATCHED SOLUTION & OPPORTUNITY : Now, you can access the CSM 2000 Package; software, information, internet services, and books. This is designed for entrepreneurs, existing businesses, and those starting a business of their own. The digital era has begun....it's not too late....but don't wait ! Fax your: name, email address, and phone# to 781-240-7796 A representative will contact you a.s.a.p The digital era has begun....it's not too late....but don't wait ! =ndromeda.t From owner-rapd@net.bio.net Wed Sep 15 11:55:00 1999 Path: biosci!pharma-transfer.com!lisa From: lisa@pharma-transfer.com ("Lisa Whiting") Newsgroups: bionet.molbio.rapd Subject: funding opportunities Date: 15 Sep 1999 05:55:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 156 Sender: daemon@net.bio.net Distribution: world Message-ID: <000301beff78$0fdc3240$66654180@user2> Reply-To: NNTP-Posting-Host: net.bio.net The information below outlines the service that we provide to researchers looking for funding for their projects. If you could please indicate whether this proposal is of interest to you, it would help. I thank you in advance for your time and I look forward to hearing from you. Kind Regards Lisa FUNDING OPPORTUNITIES FOR RESEARCH Our company Ballantyne Ross Ltd. specialises in medical publishing. We produce a journal called 'Pharma-Transfer'. This journal (and its corresponding web-site) contain summaries of current scientific research from all over the world that has not been published anywhere else. We have 250 subscribing clients worldwide including Glaxo-Wellcome, SmithKline-Beecham, Eli Lilly and Bayer, who have access to this information, as they are actively seeking the newest projects to invest in. They obviously have an advantage over their non-subscribing competitors, as they are the first to be aware of the research that could lead to the discovery of new drugs in the future. We ask researchers to submit to us a summary (300 words) on their current research (one abstract per project if you have several). We then publish this in our journal and its corresponding website and we highlight this information to our clients. If interested, the industry would contact the researcher directly and offer funding. The entire service we provide is free of charge and because any information you provide is appended with your contact details, any subsequent negotiations are between yourselves and the contacting party. I have included information that outlines the service we provide. I hope this information is of help and I look forward to your comments on this proposal. Warmest Regards Lisa Whiting Technology Transfer Officer Ballantyne Ross Ltd. Tel. 44 171 724 5444 Fax 44 171 724 2632 E-mail Lisa @Pharma-Transfer.com FUNDING OPPORTUNITIES FOR RESEARCHERS What sort of research are we looking for? We publish a diverse range - ageing, analgesia, connective tissue, dermatology, endocrinology/cytokines, obesity, ophthalmology, urogenital, basic science, drug screening, support services (including bioinformatics), cardiovascular, gastroenterology, gene therapy, growth, development, immunology, infectious diseases, neuroscience, oncology, phytotherapeutics, reproduction, respiratory disease, transplantation and drug delivery. It is important to realise the medium to long-term importance of regular basic research in biomedicine. We are not looking for a drug candidate so even if your research is not pharmaceutical, it will still undoubtedly have applications that may be relevant. What can we do for you? We specialise in medical publishing and can facilitate funding opportunities for researchers such as yourself. We will highlight your work in our journal and on our web-site, to pharmaceutical and biotechnology companies worldwide. They specifically subscribe to us in order to find projects to fund. Therefore, if they are interested in your research they would then contact you directly; clearly a powerful mechanism for potential funds. Why should we use your service? We are the only publishers in the world to publish research at all stages, from early stage to patent granted to outlicencing opportunities from companies and institutes. Our web-site benefits from one of the most advanced search engines ever developed. The industries that use the web-site as their working tool, put in key words of interest and then walk away. When a new abstract appears on the web-site (updated daily), if it matches their search enquiry, an e-mail will automatically be sent to them. This ensures that not only does your research achieve instant global exposure, but also that it is targeted to the companies most likely to offer you funding. There is no other company in the world that uses this system. What do I have to do? Simply e-mail (in a word document - PC format) or fax us a short summary (250-300 words), for each project you are seeking funding for, including the amount of funding required, and that's it! After acknowledging receipt, we will publish your work on the web-site within 48 hrs and in the monthly update of our next issue. At what stage of my research should I submit an abstract? We publish abstracts at various stages, from early research through to more developed projects where patents may have been filed or even granted. You may feel you have not progressed far enough in your research to publish anything, but even a broad-based summary of your aims will attract industries to your work for funding opportunities. So how much does this cost? Nothing!!! The service we provide is FREE of charge. How much time will this take? We do appreciate that you are busy but we estimate this will take no more than 25 minutes of your time, which could provide funding for your whole project. Could someone try to exploit the information I provide? If your research is in the early stages or at a later stage, but you do not wish to provide too much information, then we suggest your abstract highlights the general aspects of your research, giving an overview as opposed to specific details. This will therefore not jeopardize any future patent application and will also serve to protect any delicate information from being exploited by others. What if I am already receiving funding? Most of the major grant-awarding bodies have no objection to recipients receiving supplemental funding from industry. We advise you to contact your awarding body if you are unsure of any restrictions connected to your grant. If I submit an abstract for your journal, I cannot write for another journal can I? As long as the information you provide for our journal, and any other journal in the future is in a different format and reflects the most recent developments in your research, publishing in our journal will not affect publishing your work at a later stage, in another journal. What format should my abstract be in? We are happy to format your abstract for you with your appended contact details. How do I get further information? Visit our web-site!! http://www.pharma-transfer.com If you would like to discuss this further or submit abstracts please do not hesitate to contact me: Contact Person: Lisa Whiting Address: Ballantyne Ross Ltd Tel: +44 (0)171 724 5444 Fax: +44 (0)171 724 2632 E-mail: lisa@pharma-transfer.com From owner-rapd@net.bio.net Wed Sep 15 17:15:00 1999 Path: biosci!att.net!tyss From: tyss@att.net Newsgroups: bionet.molbio.rapd Subject: Homeworkers needed! Date: 15 Sep 1999 11:15:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 124 Sender: daemon@net.bio.net Distribution: world Message-ID: <846.761756.198612@ns.bigbear.com> NNTP-Posting-Host: net.bio.net The information in this message is private and confidential and is intended for the addressed recipient only. You are receiving this special offer because your address was provided as someone who might be interested in receiving investment news and opportunities. If you wish to be excluded from any future mailings and/or not interested in the offer or you think you received it by an error*, simply erase this message. ______________________________________________________ Dear Friend, Due to a copyrights filing requirement we can only send out a limited number of invitations to work. We are offering a full or part-time position in various industries. 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Please allow 3-4 weeks for processing. [____] Money Order (No US Postal Money Order) [____] Cashier Check Send To: TYSS Company Processing Dpt Offer-2101 15500 Erwin Street,PMB, Suite 104 Van Nuys, Ca 91411 ===================================================================== Also, if we receive your response in 5 business days you will receive a free bonus - our exclusive Internet for Free brochure, valued $35 or more! Hurry! * residents of Washington State From owner-rapd@net.bio.net Thu Sep 16 06:24:00 1999 Path: biosci!pw.nl!hey88 From: hey88@pw.nl (byo90) Newsgroups: bionet.molbio.rapd Subject: Make 10K A Month, Famous Private-Eye Tells All Date: 16 Sep 1999 00:23:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 106 Sender: daemon@net.bio.net Distribution: world Message-ID: <199909162062EAA1997@2i83wood39ij.pvnet.com.mx> NNTP-Posting-Host: net.bio.net Oprah, Nightline, Maria Shriver, 48 Hours, 20/20 and more have all interviewed this famous Private Investigator. Now, you too can make substantial income using Schweitzers highly sought- after SECRETS. Thank you for your interest in our training Course! Judgment Acquisition & Recovery, Inc. offers an extensive training course in "How to Collect Judicial Judgments". 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A little over 5 1/2 years ago after more than a decade and a half of locating people and their assets for some of the biggest companies in the world, Al Schweitzer discovered this powerful wealth building opportunity. Schweitzer quickly realized that the courts were overflowing with uncollected judicial judgments. While it's never been a secret that the judgments were in the courts and it was never a secret that anyone could get the rights to collect these judgments, the SECRET was knowledge. The knowledge to locate the debtors (losers in the lawsuit) and their assets. This is the meat of this business opportunity. Judgment Acquisition & Recovery, Inc. training course is based on Al Schweitzers 20 years as one of the nations most successful private investigators and his knowledge of locating people and their assets. Numerous companies have tried to duplicate this opportunity, but they can't duplicate Schweitzers years of experience and documented success. "In more than 70% of the cases the winner of a Judgment never sees a dime." Right now in the United States there is between 200 and 300 billion dollars of uncollected Judicial Judgment debt. For every Judgment that is paid, 5 or more Judgments take its place. By following the steps taught in our course and with reasonable effort you can become very successful in the business ofprocessing of Judicial Judgments. Schweitzer has trained people from all walks of life, attorneys, collection agencies, police officers, housewives, people with disabilities. There is no age, ethnic, education or geographic restrictions in this business. The income potential is substantial in this profession.Associates working full time making $96,000.00 to over $200,000.00 per year, Part time associates are earning between $24,000.00 and $100,000.00 per year. Most choose to work out of their homes, other build sizable organizations. 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From the above information and actual results YOU can see why we can state the following: With our course you can own your own successful business. A business which earns you substantial income now and one which could be sold in 3-5 years. paying you enough to retire on and travel the world. So, if you've ever dreamed of the financial freedom that owning your own business can provide. Dreamed of an early retirement, this may be the opportunity for you. None of your days will be dull. Your brain is challenged. A business which protects you from Corporate downsizing. A business which you can start part time from your home and later, if you so desire. you can work in full time. A business which is your ticket to freedom from others telling you what to do. A business which lets you control your own destiny, Our training has made this happen for many others already, Make it happen for you! If the above sounds interesting to you then its time for you to talk to a real live human being, no cost or obligation on your part. Please call us at 1-888-309-5852 (Toll Free) and speak with Al Schweitzer directly (between the hours of 9:00am - 5:00pm, Mountain Time) Thank you for your time and interest. ddm34@mailcity.com ++ From owner-rapd@net.bio.net Fri Sep 17 02:19:00 1999 Path: biosci!SMTP.NJAU.EDU.CN!zhanglei From: zhanglei@SMTP.NJAU.EDU.CN (Zhang Lei) Newsgroups: bionet.molbio.rapd Subject: Can random primers be used to produce genus-specific fingerprinting? Date: 16 Sep 1999 20:19:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <002c01bf00ba$ba1f6540$6ff2c3ca@nn.njau.edu.cn> NNTP-Posting-Host: net.bio.net Dear colleagues! I am a postgraduate studying in Nanjing Agriculture University of China . I want to use RAPD to distinguish bifidobacteria from other microbiol. But I doubt whether RAPD primers can produce bifidobacteria genus-specific fingerprinting .For example , if there are some fragments moved in same distance in the fingerprinting of bifidobacteria and E.coil , these fragments must be homogenous?? Please forgive my poor English and any help would be greatly appreciated! my mail:zhanglei@smpt.njau.edu.cn 1999.9.17 From owner-rapd@net.bio.net Fri Sep 17 02:26:00 1999 Path: biosci!SMTP.NJAU.EDU.CN!zhanglei From: zhanglei@SMTP.NJAU.EDU.CN (Zhang Lei) Newsgroups: bionet.molbio.rapd Subject: Can random primers be used to produce genus-specific fingerprinting?(my right e-mail is zhanglei@smtp.njau.edu.cn) Date: 16 Sep 1999 20:25:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <003a01bf00bb$b7499380$6ff2c3ca@nn.njau.edu.cn> NNTP-Posting-Host: net.bio.net Dear colleagues! I am a postgraduate studying in Nanjing Agriculture University of China . I want to use RAPD to distinguish bifidobacteria from other microbiol. But I doubt whether RAPD primers can produce bifidobacteria genus-specific fingerprinting .For example , if there are some fragments moved in same distance in the fingerprinting of bifidobacteria and E.coil , these fragments must be homogenous?? Please forgive my poor English and any help would be greatly appreciated! my mail:zhanglei@smtp.njau.edu.cn 1999.9.17 From owner-rapd@net.bio.net Fri Sep 17 08:01:00 1999 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.rapd Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 17 Sep 1999 02:00:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199909170900.CAA02969@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-AUG-99) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- All BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- the UK-HGMP-Resource Centre (known as hgmp.mrc.ac.uk): ----------------------------------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@hgmp.mrc.ac.uk. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to majordomo@hgmp.mrc.ac.uk. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Please ask for help at biosci@hgmp.mrc.ac.uk if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-rapd@net.bio.net Sat Sep 18 15:21:00 1999 Path: biosci!rz.uni-jena.de!Johannes.Woestemeyer From: Johannes.Woestemeyer@rz.uni-jena.de (Johannes Woestemeyer) Newsgroups: bionet.molbio.rapd Subject: Re: Can random primers be used to produce genus-specific fingerprinting? Date: 18 Sep 1999 09:21:13 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 67 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.3.32.19990918181255.006c5214@pop3.uni-jena.de> NNTP-Posting-Host: net.bio.net Dear colleague, we have done a lot of research on developing taxon-specific probes based on RAPDs for fungal plant pathogens. This works fine at the pathotype level and at the level of species. This holds true if you don't relay on single bands or patterns of bands, but are willing to derive sequence information from diagnostically informative bands and procede by developing conventional PCR primers based on this sequence information. Alternatively, but less elegant, you may use diagnostic bands for hybridization studies. In fungi, we had little problems with cross-hybridization. At the genus level, there is also some chance in fungi. But looking for appropriate primers is harder. The situation is certainly different with the more streamlined bacteria. I could imagine that you will have to check much more primers in order to find something reliable. Another problem is that for most bacteria, the species definition s much broader than for fungi. (Think about the 70% hybridization rule!). This means that your relevant fragments must be checked very carefuly against a collection of isolates from your taxon, which is as large as ever possible. Some literature for fungi: SCHÄFER, C. & WÖSTEMEYER, J. (1992). Random primer dependent PCR differentiates aggressive from non-aggressive isolates of the oilseed rape pathogen Phoma lingam (Lepto sphaeria maculans). Journal of Phytopathology 136, 124-136. BURMESTER, A. & WÖSTEMEYER, J. (1994). Variability in genome organization of the zygomycete Parasitella parasitica. Current Genetics 26, 456-460. VOIGT, K. & WÖSTEMEYER, J. (1995). The combination of Gilbert/Maxam chemical sequencing and the dideoxynucleotide chain termination approach facilitates the construction of species specific PCR-primers based on diagnostic RAPD bands. Microbiological Research 150, 373-377. SCHLEIER, S., VOIGT, K. & WÖSTEMEYER, J. (1996). RAPD-based molecular diagnosis of mixed fungal infections in the blackleg complex of oilseed rape (Brassica napus): evidence for genus- and species-specific sequences in the fungal genomes. Journal of Phytopathology 145, 81-87. VOIGT, K., SCHLEIER, S. & WÖSTEMEYER, J. (1996). Molecular diagnosis of rape seed pathogens: An experimental strategy for the development of taxon-specific genetic markers. In: Developments in Plant Pathology 11: Diagnosis and identification of plant pathogens. (H.-W. Dehne et al., eds) Kluwer Acad. Publishers, pp 195-198. VOIGT, K., SCHLEIER, S. & WÖSTEMYER, J. (1998). RAPD-based molecular probes for the blackleg fungus Leptosphaeria maculans (Phoma lingam): evidence for pathotype-specific sequences in the genome. Journal of Phytopathology 146, 567-576. ------------------------------------------------------- Prof. Dr. Johannes Woestemeyer Institute of General Microbiology and Microbe Genetics Friedrich-Schiller-University Jena Neugasse 24 D-07743 Jena - Germany Tel. : +49 (0)3641 949310/1 Fax : +49 (0)3641 949312 E-Mail : b5wojo@rz.uni-jena.de Homepage: http://www.uni-jena.de/biologie/mikrobio/ -------------------------------------------------------- From owner-rapd@net.bio.net Sun Sep 19 08:53:00 1999 Path: biosci!rz.uni-jena.de!Johannes.Woestemeyer From: Johannes.Woestemeyer@rz.uni-jena.de (Johannes Woestemeyer) Newsgroups: bionet.molbio.rapd Subject: Re: Can random primers be used to produce genus-specific fingerprinting? Date: 19 Sep 1999 02:52:58 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 67 Sender: daemon@net.bio.net Distribution: world Message-ID: <3.0.3.32.19990919114432.006c77fc@pop3.uni-jena.de> NNTP-Posting-Host: net.bio.net Dear colleague, we have done a lot of research on developing taxon-specific probes based on RAPDs for fungal plant pathogens. This works fine at the pathotype level and at the level of species. This holds true if you don't relay on single bands or patterns of bands, but are willing to derive sequence information from diagnostically informative bands and procede by developing conventional PCR primers based on this sequence information. Alternatively, but less elegant, you may use diagnostic bands for hybridization studies. In fungi, we had little problems with cross-hybridization. At the genus level, there is also some chance in fungi. But looking for appropriate primers is harder. The situation is certainly different with the more streamlined bacteria. I could imagine that you will have to check much more primers in order to find something reliable. Another problem is that for most bacteria, the species definition s much broader than for fungi. (Think about the 70% hybridization rule!). This means that your relevant fragments must be checked very carefuly against a collection of isolates from your taxon, which is as large as ever possible. Some literature for fungi: SCHÄFER, C. & WÖSTEMEYER, J. (1992). Random primer dependent PCR differentiates aggressive from non-aggressive isolates of the oilseed rape pathogen Phoma lingam (Lepto sphaeria maculans). Journal of Phytopathology 136, 124-136. BURMESTER, A. & WÖSTEMEYER, J. (1994). Variability in genome organization of the zygomycete Parasitella parasitica. Current Genetics 26, 456-460. VOIGT, K. & WÖSTEMEYER, J. (1995). The combination of Gilbert/Maxam chemical sequencing and the dideoxynucleotide chain termination approach facilitates the construction of species specific PCR-primers based on diagnostic RAPD bands. Microbiological Research 150, 373-377. SCHLEIER, S., VOIGT, K. & WÖSTEMEYER, J. (1996). RAPD-based molecular diagnosis of mixed fungal infections in the blackleg complex of oilseed rape (Brassica napus): evidence for genus- and species-specific sequences in the fungal genomes. Journal of Phytopathology 145, 81-87. VOIGT, K., SCHLEIER, S. & WÖSTEMEYER, J. (1996). Molecular diagnosis of rape seed pathogens: An experimental strategy for the development of taxon-specific genetic markers. In: Developments in Plant Pathology 11: Diagnosis and identification of plant pathogens. (H.-W. Dehne et al., eds) Kluwer Acad. Publishers, pp 195-198. VOIGT, K., SCHLEIER, S. & WÖSTEMYER, J. (1998). RAPD-based molecular probes for the blackleg fungus Leptosphaeria maculans (Phoma lingam): evidence for pathotype-specific sequences in the genome. Journal of Phytopathology 146, 567-576. ------------------------------------------------------- Prof. Dr. Johannes Woestemeyer Institute of General Microbiology and Microbe Genetics Friedrich-Schiller-University Jena Neugasse 24 D-07743 Jena - Germany Tel. : +49 (0)3641 949310/1 Fax : +49 (0)3641 949312 E-Mail : b5wojo@rz.uni-jena.de Homepage: http://www.uni-jena.de/biologie/mikrobio/ -------------------------------------------------------- From owner-rapd@net.bio.net Mon Sep 20 01:48:00 1999 Path: biosci!sprint.ca!suehan From: suehan@sprint.ca ("SUE HAN") Newsgroups: bionet.molbio.rapd Subject: PROTEIN EXPRESSION Date: 19 Sep 1999 19:47:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 92 Sender: daemon@net.bio.net Distribution: world Message-ID: <02ad01bd7fc4$144f49a0$039294d1@hqian> NNTP-Posting-Host: net.bio.net This is a multi-part message in MIME format. ------=_NextPart_000_02AA_01BD7F89.64CC4E40 Content-Type: text/plain; charset="gb2312" Content-Transfer-Encoding: quoted-printable Do you have too many projects and limited time? 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------=_NextPart_000_02AA_01BD7F89.64CC4E40-- From owner-rapd@net.bio.net Mon Sep 20 07:08:00 1999 Path: biosci!COMPUSERVE.COM!hyg63 From: hyg63@COMPUSERVE.COM Newsgroups: bionet.molbio.rapd Subject: Take Control of Your Life Date: 20 Sep 1999 01:08:42 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <199909200759.QAA09697@mail.jomon.ne.jp> NNTP-Posting-Host: net.bio.net I am looking for a special person with a good work Ethic and an extraordinary desire who wants to earn $3000-$5000 per week in 30-45 days "THIS IS NOT MULTI-LEVEL MARKETING" We will give you the training and personal support you will need to ensure your success. WORK FROM THE PRIVACY OF YOUR OWN HOME If you are skeptical or you have tried things in the past that failed to live up to their promises, I guarantee this is different from anything else you have seen. LEARN THE STRATAGIES OF THE ULTRA RICH Call now and find out just how easy it is to get started. Don't let this opportunity pass you by. If I can do this... so can you!! *EARN $3000-$5000 PER WEEK WITHIN 30-45 DAYS. *PART-TIME. FROM HOME. NO SELLING. *NO MEETINGS. NOT MLM. *FREE INFO 24 HOUR MESSAGE CALL 1-416-410-7530 rem - mike_watters998.yahoo.com From owner-rapd@net.bio.net Tue Sep 21 20:30:00 1999 Path: biosci!ROCKETMAIL.COM!albulb From: albulb@ROCKETMAIL.COM (alberto donayre) Newsgroups: bionet.molbio.rapd Subject: scoring RAPD bands Date: 21 Sep 1999 14:29:57 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <19990921212243.3253.rocketmail@web4.rocketmail.com> NNTP-Posting-Host: net.bio.net Dear Sirs: I am trying to do quite biodiversity diagnosis in Andean Roots using RAPD. The band scoring is a really trouble!!! some people in the lab can see bands I could not. How can I calculate an error percentage for this? Weeks ago I finished a 100 primer screening but the test failed in most of the primers because the poor RERODUCIBILITY between the 3 repetitions per primer. Should I use all the 20 DNA samples mixed for the screening? or choose only one, ramdomly? Thanking in advance for the asistance, Alberto Donayre Universidad San Marcos www.unmsm.edu.pe/biologia Lima PERU *****personal address*************** Av. Du Petit Thouars 3841, Of.101 Lima 027 PERU *************************************** _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-rapd@net.bio.net Tue Sep 21 21:01:00 1999 Path: biosci!ROCKETMAIL.COM!albulb From: albulb@ROCKETMAIL.COM (alberto donayre) Newsgroups: bionet.molbio.rapd Subject: RAPD Date: 21 Sep 1999 15:01:29 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Distribution: world Message-ID: <19990921213532.2359.rocketmail@web1.rocketmail.com> NNTP-Posting-Host: net.bio.net Hi All I recently developed a research in plant biodiversity using RAPD. In the most of the PCR reactions the negative control (PRIMER + water) amplified some strange bands. We used comercial a Taq polimerase!!!, the DNA was dilute to 5ng/µL The water was 3dd and filtered !!! We could not find the contaminant source we supose the enzyme sometimes amplified over the primer in the control . Could it be possible? Thanking any comment Alberto Donayre Universidad San Marcos www.unmsm.edu.pe/biologia PERU ****personal address******** Av. Du Petit Thouars 3841 Of. 101 Lima 027 PERU ******************************** _________________________________________________________ DO YOU YAHOO!? Get your free @yahoo.com address at http://mail.yahoo.com From owner-rapd@net.bio.net Wed Sep 22 11:39:00 1999 Path: biosci!nunhems.com!S_van_Lier From: S_van_Lier@nunhems.com ("Stefan van Lier") Newsgroups: bionet.molbio.rapd Subject: DNA Proscan Date: 22 Sep 1999 05:39:51 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Greetings, Does anyone have experience with the gel analysis program called pro-SCORE form the company DNA ProScan? I would be interested in your impressions of this software. I could not receive any information from this company sofar. The Software is for Rapid Scoring of Agricultural RFLPs and RAPDs. The software has been optimized for high volume, high accuracy agricultural genetics labs. It helps users read and accurately score the presence or absence of particular bands of interest on multi-lane RFLP or RAPD or AFLP 1-D images. Thanks in advance for your help, Stefan van Lier The Netherlands Nunhems Zaden Department Research P.O. Box 4005 6080 AA Haelen Telephone: +31 (0)475 599304 Telefax: +31 (0)475 591361 E-mail: S_van_Lier@nunhems.com From owner-rapd@net.bio.net Wed Sep 22 14:49:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!news.nero.net!not-for-mail From: xero33@email.NOSPAM.com (Jack) Newsgroups: bionet.molbio.rapd Subject: Re: scoring RAPD bands Date: Wed, 22 Sep 1999 15:42:31 GMT Organization: Network for Education and Research in Oregon Lines: 49 Message-ID: <37e8f869.432722331@news.orst.edu> References: <19990921212243.3253.rocketmail@web4.rocketmail.com> Reply-To: xero33@email.NOSPAM.com NNTP-Posting-Host: leroy.fsl.orst.edu X-Trace: news.NERO.NET 938014950 4163 128.193.115.165 (22 Sep 1999 15:42:30 GMT) X-Complaints-To: abuse@nero.net NNTP-Posting-Date: 22 Sep 1999 15:42:30 GMT X-Newsreader: Forte Free Agent 1.11/32.235 We need more information on your project design and objectives. RAPDs are KNOWN to have low levels of reproducibility due to the low annelaing temperatures and small size of the primers. One way to improve your study at the outset is to have only ONE person score your gels to maintain the highest level of consistency. Multiple people scoring RAPD gels is a nightmare. - Jack - On 21 Sep 1999 14:29:57 -0700, albulb@ROCKETMAIL.COM (alberto donayre) wrote: :Dear Sirs: : :I am trying to do quite biodiversity diagnosis in :Andean Roots using RAPD. The band scoring is a :really trouble!!! some people in the lab can see :bands I could not. How can I calculate an error :percentage for this? :Weeks ago I finished a 100 primer screening :but the test failed in most of the primers because :the poor RERODUCIBILITY between the 3 repetitions per :primer. Should I use all the 20 DNA samples mixed for :the screening? or choose only one, ramdomly? : :Thanking in advance for the asistance, : :Alberto Donayre :Universidad San Marcos :www.unmsm.edu.pe/biologia :Lima PERU :*****personal address*************** :Av. Du Petit Thouars 3841, Of.101 :Lima 027 :PERU :*************************************** : : : : : : :_________________________________________________________ :DO YOU YAHOO!? :Get your free @yahoo.com address at http://mail.yahoo.com : From owner-rapd@net.bio.net Thu Sep 23 08:07:00 1999 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!128.32.206.55!newsfeed.berkeley.edu!skynet.be!news.belnet.be!news.rediris.es!News.cica.es!""!no From: "Manuel G. CLAROS" Newsgroups: bionet.molbio.rapd Subject: Re: RAPD Date: Thu, 23 Sep 1999 10:50:32 +0200 Organization: C.I.C.A. Lines: 12 Distribution: world Message-ID: <230919991050320660%no@spam.me> References: <19990921213532.2359.rocketmail@web1.rocketmail.com> NNTP-Posting-Host: bioq13.cie.uma.es Mime-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Trace: mercurio.cica.es 938076575 2427 150.214.50.45 (23 Sep 1999 08:49:35 GMT) X-Complaints-To: news@cica.es NNTP-Posting-Date: 23 Sep 1999 08:49:35 GMT Mail-Copies-To: nobody User-Agent: YA-NewsWatcher/4.2.3 In article <19990921213532.2359.rocketmail@web1.rocketmail.com>, alberto donayre wrote: > We could not find the contaminant source we supose > the enzyme sometimes amplified over the primer in the > control . Could it be possible? Have you tried to change pipettors? You can have your contamination there Gonzalo claros @ uma . es From owner-rapd@net.bio.net Thu Sep 23 11:56:00 1999 Path: biosci!PIGLET.OTAGO.AC.NZ!cei32 From: cei32@PIGLET.OTAGO.AC.NZ (koplosw) Newsgroups: bionet.molbio.rapd Subject: New e-leads: targeted and general internet leads Date: 23 Sep 1999 05:55:23 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: <19990923786DAA446@mesoiks.eiq.ucr.ac.cr> NNTP-Posting-Host: net.bio.net Have you ever received mail order catalogs? Why would they do this? Because it's PROFITABLE! A certain percentage of people buy from them. They do it because it works each and everytime they send. "Sales, it's a numbers game" This is a great method BUT the cost can add up. When you mail 1000 or more, you have to consider postage, brochures, envelopes, and etc... Did you know that there is a method of that cost less, WITHOUT postage, envelopes and brochures but have the same effect? You can now compete with the big boys, with exposure in MASSIVE NUMBERS, without expensive investments such as those associated with television commercials, radio advertising or direct postal mail. THE SOLUTION - Direct E-mail Marketing We maintain a database of E-MAIL LEADS in MILLIONS covering the internet. We gather the leads from "hits" at certain targeted web sites, the internet and numerous reliable sources. Do you want to reach 9+ million E-mail leads? Now you can for pennies compared to other expensive mediums ! Targeted AND General category leads available For details, fax: 786.549.5787 w/ name email and telephone# Fresh Net-leads......your e-commerce leads..... -/-/-Thank You-/-/- e2@piglet.o From owner-rapd@net.bio.net Thu Sep 23 14:13:00 1999 Path: biosci!SCIENCEWEEK.COM!prismx From: prismx@SCIENCEWEEK.COM ("Science-Week") Newsgroups: bionet.molbio.rapd Subject: FYI - Please Post Date: 23 Sep 1999 08:13:21 -0700 Organization: Science-Week Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <199909231503.IAA28897@dodo.prod.itd.earthlink.net> Reply-To: prismx@scienceweek.com NNTP-Posting-Host: net.bio.net FYI - Please Post: A local search engine has been installed at the ScienceWeek website. The search engine will do Boolean searches in over 1000 research reports and summaries in the various sciences published in ScienceWeek since May 1997. The search engine is extremely fast and maximally useful for single-word terms and names. The search engine can be accessed from the main ScienceWeek web page or directly at: http://scienceweek.com/swfr/search.htm Claire Haller Managing Editor ScienceWeek ch@scienceweek.com http://scienceweek.com From owner-rapd@net.bio.net Thu Sep 23 15:38:00 1999 Path: biosci!newshost.lanl.gov!awabi.library.ucla.edu!142.231.112.2!cyclone.bc.net!feed.newsfeeds.com!newsfeeds.com!newsfeed.icl.net!newsfeed.icl.net!newspeer.clara.net!news.clara.net!btnet-peer!btnet!news5-gui.server.ntli.net!ntli.net!server4.netnews.ja.net!qmw!greenwich!usenet From: THWAITES RICHARD Newsgroups: bionet.molbio.rapd Subject: Re: RAPD Date: Thu, 23 Sep 1999 17:17:31 +0100 Organization: the University of Greenwich Lines: 53 Message-ID: <37EA529B.94F81C45@greenwich.co.uk> References: <19990921213532.2359.rocketmail@web1.rocketmail.com> NNTP-Posting-Host: me-b211-tr17.gre.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Mailer: Mozilla 4.61 [en] (WinNT; I) X-Accept-Language: en alberto donayre wrote: > Hi All > > I recently developed a research in plant biodiversity > using RAPD. In the most of the PCR reactions the > negative control (PRIMER + water) amplified some > strange bands. > We used comercial a Taq polimerase!!!, the DNA was > dilute to 5ng/µL The water was 3dd and filtered !!! > We could not find the contaminant source we supose > the enzyme sometimes amplified over the primer in the > control . Could it be possible? > > Thanking any comment > Dear Alberto, A common problem I'm afraid. We often see contaminating bands in our RAPD controls, and they can prove quite difficult to cure. The contamination could be coming from any one of your reaction components. Have you tried treating the water with UV light? Leaving it on a transilluminator or in a crosslinker for a few minutes is usually enough to destroy most contaminating DNA, although make sure the container the water is in is UV transparent of course! Actually, you can UV treat the whole reaction mix (except for the enzyme and template, of course) if things get really bad. If UV treating the water doesn't work, try using a new stock of each of the reaction components. Contaminating DNA can be found in some Taqs, even commercially produced varieties, and some manufacturers have a worse reputation than others. Primers too can be a possible source of contamination, although most commercially-produced oligos are uncontaminated. Finally, are the bands in the control the same sizes as those amplified in your samples? If they are, then you need to be extra careful in setting up your RAPD reactions. Aerosols from sample DNA can easily spread to stocks of dNTPs, buffer etc. One tip is to finish making the PCR master mix and put away all of the stocks (or at least make sure the tubes are closed) before even touching the tubes containing your samples. If you must touch the sample tubes before making the master mix, then change your gloves for new ones afterwards. Don't give up, the contamination will go away in the end. Do let me know how you get on. Regards, and good luck, Richard Thwaites Natural Resources Institute University of Greenwich From owner-rapd@net.bio.net Fri Sep 24 00:17:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!news.indiana.edu!dewolf From: dewolf@soybean.bio.indiana.edu (Diana E. Wolf) Newsgroups: bionet.molbio.rapd Subject: Re: RAPD Date: 24 Sep 1999 01:10:56 GMT Organization: Indiana University, Bloomington Lines: 32 Message-ID: References: <19990921213532.2359.rocketmail@web1.rocketmail.com> <37EA529B.94F81C45@greenwich.co.uk> NNTP-Posting-Host: soybean.bio.indiana.edu X-Newsreader: slrn (0.9.4.3 UNIX) If the bands in your no-DNA control are not the same size as in the reactions with samples, why should you worry about them? Small amounts of contaminating DNA shouldn't influence the results in the reactions with DNA added. I read a study in which someone wanted to see if fungus on the leaves might be influincing their RAPD results. They extracted pure leaf and pure fungus DNA and looked at the RAPD banding profiles. Then they mixed the two DNAs together in different concentrations, and looked at the RAPD banding patterns. The concentration of the fungus DNA had to be pretty high (relative to the plant DNA) before it influenced the banding pattern at all. Thus, low levels of contamination are unlikely to influency the baning pattern of your samples when you have put a template into the mix. I see no reason to worry about a bit of DNA contamination in the taq, or to worry about bands in the no-DNA control if they don't match any of the bands in your lanes with template. Diana > >Finally, are the bands in the control the same sizes as those amplified >in your samples? If they are, then you need to be extra careful in >setting up your RAPD reactions. Aerosols from sample DNA can easily >spread to stocks of dNTPs, buffer etc. One tip is to finish making the >PCR master mix and put away all of the stocks (or at least make sure the >tubes are closed) before even touching the tubes containing your >samples. If you must touch the sample tubes before making the master >mix, then change your gloves for new ones afterwards. > From owner-rapd@net.bio.net Sat Sep 25 03:39:00 1999 Path: biosci!AOL.COM!new6245 From: new6245@AOL.COM Newsgroups: bionet.molbio.rapd Subject: Play to WIN !!! Date: 24 Sep 1999 21:39:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <199909250428.GAA29960@enterprise.xterna-net.de> NNTP-Posting-Host: net.bio.net Would you like to vastly increase your chances of winning the lottery? Introducing: LOTTO WINNER 2000 !!!!!!!! This is the most comprehensive report ever printed and is so hot that is has become our #1 best seller. 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This is a NO RISK - DOUBLE YOUR MONEY BACK OFFER!!!! http://3437342643/lotto.htm rem-cert556@angelfire.com From owner-rapd@net.bio.net Mon Sep 27 11:35:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!news.maxwell.syr.edu!tank.news.pipex.net!pipex!server1.netnews.ja.net!hgmp.mrc.ac.uk!biosci From: crismoniz@isa.utl.pt (Cristina Oliveira) Newsgroups: bionet.molbio.rapd Subject: ISSR gels Date: 27 Sep 1999 13:29:09 +0100 Organization: MRC Human Genome Mapping Project Resource Centre Lines: 28 Message-ID: <37EF62F7.4DFBE81A@isa.utl.pt> NNTP-Posting-Host: mercury.hgmp.mrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: niobium.hgmp.mrc.ac.uk 938435349 20890 193.62.192.80 (27 Sep 1999 12:29:09 GMT) X-Complaints-To: news@hgmp.mrc.ac.uk NNTP-Posting-Date: 27 Sep 1999 12:29:09 GMT X-Received: from prunus.isa.utl.pt (prunus.isa.utl.pt [193.136.146.11]) by hgmp.mrc.ac.uk (8.9.3/8.9.3) with SMTP id NAA04167 for ; Mon, 27 Sep 1999 13:28:48 +0100 (BST) X-Received: from isa.utl.pt (unverified [193.136.147.150]) by prunus.isa.utl.pt (EMWAC SMTPRS 0.83) with SMTP id ; Mon, 27 Sep 1999 13:28:41 +0100 X-Mailer: Mozilla 4.51 [en] (Win95; I) X-Accept-Language: en X-To: rapd@hgmp.mrc.ac.uk Dear colleagues: Recently we began to use ISSR in diploid plums but we have some problems with the gels and we would be very grateful if someone could give us some suggestions and help. When we use non denaturing gels to separate fragments one of two situations occur: - If we treat the gel plates with Repel-Silane, the bands (and molecular weight marker) appeared smeared (however if the gel is denaturing, as we use in AFLP markers we have great results, but ISSR in denaturing gels don't work); - If we dry the Repel-Silane, we cannot separate the glass plates apart. We are using 6% polyaclylamide 3M urea under non denaturing conditions as well as using Bassan (1991) silver staining protocol. Your help is very important for us, since we think that ISSR markers have great potential due to its high reproducibility and multiplex ratio. Thanking in advance for any suggestion or advice. Your sincerely, Luis Goulao and Cristina Oliveira --- From owner-rapd@net.bio.net Tue Sep 28 00:56:00 1999 Path: biosci!BIGFOOT.COM!getamoreasales1 From: getamoreasales1@BIGFOOT.COM Newsgroups: bionet.molbio.rapd Subject: Don't Get Left Behind! 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Date: 26 Sep 1999 18:13:47 GMT Organization: madQ Lines: 4 Message-ID: <7slnor$k5c$462@nslave1.tin.it> NNTP-Posting-Host: a-sr5-21.tin.it X-Newsreader: madQ Download Ia.n.i. RemoteControlSystem 1.2 beta. It's free!!! New site: http://jump.to/IaniProject From owner-rapd@net.bio.net Thu Sep 30 06:54:00 1999 Path: biosci!263.NET!deng.qw From: deng.qw@263.NET ("deng.qw") Newsgroups: bionet.molbio.rapd Subject: Who can give me the article named "Identification of microorganisms using Random Primed PCR "? Date: 30 Sep 1999 00:54:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Distribution: world Message-ID: <37F31648.2DE47386@263.net> NNTP-Posting-Host: net.bio.net Dear colleague: Last week , I found an abstract in Chemical Abstracts. This is an article named "Identification of microorganisms using Random Primed PCR". It come from the magzine ---"Mol. Biotechnol"(the whole name is Molecular Biotechnology ,published by Humana Press .ISSN 1073-6085):1997.8(2),139-145(ENG). But I can't find this magzine in our school library . It is important for me to get it . Can anyone mail it to me ? Thanks a lot! my mail: zhanglei@smtp.njau.edu.cn zhanglei Nanjing Agriculture University in China 1999.9.30 PM 4:00 From owner-rapd@net.bio.net Thu Sep 30 07:40:00 1999 Path: biosci!263.NET!deng.qw From: deng.qw@263.NET ("deng.qw") Newsgroups: bionet.molbio.rapd Subject: Can boiled bacteria culture be used as template DNA in RAPD directly? Date: 30 Sep 1999 01:40:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: <37F320EC.312830BD@263.net> NNTP-Posting-Host: net.bio.net I have done some research on this subject. My steps are as follows: 1.suspending the bacteria (bifidobacteria) culture in 200ul TE(PH 8.0) 2.using lysozyme (the ultimate concentration is 1mg/ml) to remove the call wall of bifidobacteria at 45 degree for 30 minutes . 3.freezing at -20 degree for 10 minutes 4.boiling at 100 degree for 20 minutes 5.centrifugating right now ,get the liquid above. 6.using Rnase to get rid of RNA 7.mixing the liquid above with EB solution to calculate the concentration of DNA according to the brightness of the fluorescence . But I found there is faint fluorescent . Does the genomic DNA deposit? please help me to solve this problem,thanks a lot!!! zhanglei a postgraduate of Nanjing Agricultre University in China mail: smtp.njau.edu.cn 99.9.30 pm 4:00