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RESUME
ZHU Qingsong

Beijing Forestry University #154
Beijing 100083
China
Email: qszhu@ss2.bjfu.edu.cn

PROFESSIONAL OBJECTIVE
Seeking a position as a research assistant or a Ph. D. student

SKILLS

Practical and theoretical knowledge of molecular biology & biotechnology
including:

Tissue and Cell Culture of Plant
Plant Transformation Using Agrobacterium
Gene Expression in E. coli
PCR
RAPD
DNA and RNA Isolation, Extraction and Purification
Ligation and Bacterium Transformation
Construction of cDNA Library
Southern and Northern Blotting
Protein Electrophoresis (one-dimension and two-dimension)
Gas Chromatography and Liquid Chromatography

Competent with most desktop computer applications (Microsoft Office,
Adobe PhotoShop, FrontPage, Visual Basic and
HTTM Language).

EXPERIENCE
Lecturer & Laboratory Instructor of Molecular Biology, July, 1999 -
Present, College of Plant Sciences, Beijing Forestry
University

Research Assistant, September, 1996 - July, 1999, College of Plant
Sciences, Beijing Forestry University
Focusing on High-level Expression of ACC Synthase in E. coli

Assistant Researcher, July, 1993 - September, 1996, Experimental Center
of Forestry Biology, Beijing Forestry University
Tissue and Cell Culture of Trees; Transformation of Trees by
Agrobacterium

EDUCATION
Master of Science, September, 1996 - July, 1999, College of Plant
Sciences, Beijing Forestry University

Certificate, 1995, Institute of Development, Academia Sinica
Course: Latest Experimental Techniques of Molecular Biology

Certificate, 1994, College of Life Sciences, Peking University
Course: Biochemistry and Molecular Biology

Certificate, 1993, Institute of Biophysics, Academia Sinica
Course: Electrophoresis Techniques of Protein

Certificate, 1993, Academia Sinica of Agriculture
Course: Molecular Hybridization Techniques

Bachelor of Forestry Sciences, September, 1989 - July, 1993, College of
Resources & Environment, Beijing Forestry
University

ASSOCIATIONS
Chinese Association of Plant Physiology
Chinese Association of Botany

DATE OF BIRTH
February 13, 1971

CITIZENSHIP
People's Republic of China

REFERENCES
Professional and personal references available upon request.


---


From owner-rapd@hgmp.mrc.ac.uk  Sun Dec  5 23:08:06 1999
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From: prismx@SCIENCEWEEK.COM ("Science-Week")
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Subject: ScienceWeek - NEW FREE REPORTS 5 DEC 99
Date: 5 Dec 1999 14:56:58 -0800
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FYI:

The following new free reports have been posted at the ScienceWeek
website (links on the main page):

On the Genetic Code vs. Human Language
On Aesthetics as a Guide to Theory
Astrophysics: Large-Scale Structure in the Universe
Cell Biology: Structural Mechanisms of Endocytosis
Neurobiology: Estrogen and Human Brain Activity
On DNA Vaccines
Molecular Biology: Protein Folding

These reports are from back issues of ScienceWeek. They can be
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A Reminder: The SW website now contains a local search engine
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The Editors
ScienceWeek
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From owner-rapd@hgmp.mrc.ac.uk  Sun Dec 12 01:33:08 1999
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From: cay88@goose.bitecomm.co.uk (lsmirld)
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Subject: ADV:.Free..Gift -- aging, wight-loss, energy
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From owner-rapd@hgmp.mrc.ac.uk  Mon Dec 13 10:39:05 1999
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FYI:

The following new free reports have been posted at the ScienceWeek
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Earth Science: The Layered Earth
Particle Physics: Nucleon Spin
Earth Science: On the Possibility of Rapid Climate Change
Materials Science: A New Approach to Materials Design
Developmental Biology: Notch Signaling
Neurobiology: Potassium Channel Gating
Science Policy: Basic Energy Research

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---


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PLEASE POST
----------------------

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From owner-rapd@hgmp.mrc.ac.uk  Fri Dec 17 04:45:23 1999
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From: "moj-taba" <moj-taba@email.msn.com>
Subject: SCARs
Date: Thu, 16 Dec 1999 20:44:21 -0800
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Dear RAPD Subscribers:
I have screened several RAPD primers between two different genomic DNA for
polymorphisms. In order to design Sequence Characterize Amplified Region
(SCARs), the polymorphic bands were gel isolated and sequenced. Longer,
18-20mer, oligos were designed. Each forward and reverse oligo contained the
1st ten bases of the original random primer. The SCARs failed to produce the
same polymorphisms obtained through RAPD. Optimization of the reaction
conditions, such as increasing the annealing temperature and lowering the
MgCl concentration, did not help. Can anyone explain why did this happen?
What are the possible theoretical reasons behind this? Thanks for your
comments.
Mojtaba





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From owner-rapd@hgmp.mrc.ac.uk  Sun Dec 19 16:13:38 1999
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From owner-rapd@hgmp.mrc.ac.uk  Mon Dec 20 10:41:47 1999
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From: Johannes.Woestemeyer@rz.uni-jena.de (Johannes Woestemeyer)
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 Mojtaba wrote:

"have screened several RAPD primers between two different genomic DNA for 
polymorphisms. In order to design Sequence Characterize Amplified Region 
(SCARs), the polymorphic bands were gel isolated and sequenced. Longer, 
18-20mer, oligos were designed. Each forward and reverse oligo contained the 
1st ten bases of the original random primer. The SCARs failed to produce the 
same polymorphisms obtained through RAPD. Optimization of the reaction 
conditions, such as increasing the annealing temperature and lowering the 
MgCl concentration, did not help. Can anyone explain why did this happen? 
What are the possible theoretical reasons behind this? Thanks for your 
comments. "

Dear colleague,

our experiences with SCARS from polymorphic and diagnostic RAPD bands are
much better. We developed some 20 taxon-specific probes by this method.
What we did, however, was to check diagnostic EAPD bands first by Southern
hybridization prior to the sequencing work. Not all polymorphic bands pass
this test. Maybe you should adjust your protocol correspondingly. Another
possible experimental fault: Maybe that you did not really clone and
sequence the true band. Did you check?

Johannes Woestemeyer

Prof. Dr. Johannes Woestemeyer
Friedrich-Schiller-Universitaet Jena
Lehrstuhl fuer Allgemeine Mikrobiologie und Mikrobengenetik
Neugasse 24 - D - 03641 Jena

Tel: +49 (0)3641 949311/0   Fax: +49 (0)3641 949312
Email: b5wojo@rz.uni-jena.de   
Web site: http://www.uni-jena.de/biologie/mikrobio/
---


From owner-rapd@hgmp.mrc.ac.uk  Wed Dec 22 00:28:06 1999
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From: albulb@rocketmail.com (alberto donayre)
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Dear RAPD Subscribers 
Dr. Moj-Taba :
I need a reference wich ilustrate how to recover DNA
of markers from the agarose gel in order to desing the
SCAR's oligos. Could you briefly explain me and/or
sending me references about?
I will appreciate any comment

Regards

Alberto Donayre
Universidad San Marcos/Biologia
http://www.unmsm.edu.pe/biologia
Lima PERU

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From owner-rapd@hgmp.mrc.ac.uk  Wed Dec 22 12:14:58 1999
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The sequence polymorphism is probably a point mutation. The longer specific
SCAR-Primer can anneal though there is this sequence difference.
In our experience only very few RAPDs can be changed in SCARs. More infos in the
original paper of paran and michelmore.
Mark

moj-taba schrieb:

> Dear RAPD Subscribers:
> I have screened several RAPD primers between two different genomic DNA for
> polymorphisms. In order to design Sequence Characterize Amplified Region
> (SCARs), the polymorphic bands were gel isolated and sequenced. Longer,
> 18-20mer, oligos were designed. Each forward and reverse oligo contained the
> 1st ten bases of the original random primer. The SCARs failed to produce the
> same polymorphisms obtained through RAPD. Optimization of the reaction
> conditions, such as increasing the annealing temperature and lowering the
> MgCl concentration, did not help. Can anyone explain why did this happen?
> What are the possible theoretical reasons behind this? Thanks for your
> comments.
> Mojtaba

--
    -------------------------
    Mark von Stackelberg
    Lehrgebiet Molekulargenetik
    Universitaet Hannover
    Herrenhaeuser Str. 2
    D-30419 Hannover
    ---------------------------
    Fon : +49 (0)511 7624037
    Fax : +49 (0)511 7624088
    ---------------------------




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From: albulb@rocketmail.com (alberto donayre)
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Subject: Papers request
Date: 27 Dec 1999 17:25:33 -0000
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Dear Rapd List:

Could somebody send me a simple xerox copy of the
following papers, please?

Haley SD, Miklas PN, Stavely JR, Byrum J and Kelly JD.
(1993) Identification of a RAPD markers linked to rust
resistance gene block in common bean. Theor Appl Genet
85:745-749
******************************************************
Paran I. and Michelmore R.. (1993) Development of
reliable PCR-based markers linked to downy mildew
resistance genes in lettuce. Theor Appl Genet
85:985-993
******************************************************
Yin TM, Huang M, Wang MX, Zhu LH and Zhai WX (1996)
Construction of Molecular linkage map in masson pine
using RAPD markers and megagametophytes from a single
tree. Acta Botanica Sinica 39(7): 607-612


I will apprecite your assistance
Thanks in advance

Alberto Donayre
http://www.unmsm.edu.pe/biologia
Lima PERU
********PERSONAL ADDRESS*********
Av.Du Petit Thouars 3841, Of.101
Lima 027
PERU
********************************





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From owner-rapd@hgmp.mrc.ac.uk  Tue Dec 28 19:37:22 1999
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From: albulb@rocketmail.com (alberto donayre)
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Subject: how many primers for RAPD?
Date: 28 Dec 1999 19:37:18 -0000
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Dear colleagues,

We developed  RAPD with some primers
the question is, How many primers for RAPD
should I use? I read it depend on the genome 
complexity...?
we did a 107 prior screening

Thanks in advance,

Alberto
http://www.unmsm.edu.pe/biologia
Lima PERU




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From owner-rapd@hgmp.mrc.ac.uk  Tue Dec 28 21:45:16 1999
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From: dewolf@soybean.bio.indiana.edu (Diana E. Wolf)
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Subject: Re: how many primers for RAPD?
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On 28 Dec 1999 19:37:18 -0000, alberto donayre <albulb@rocketmail.com> wrote:

It depends on what you are trying to do with the data.
Perhaps you could fill us in?
Diana

>Dear colleagues,
>
>We developed  RAPD with some primers
>the question is, How many primers for RAPD
>should I use? I read it depend on the genome 
>complexity...?
>we did a 107 prior screening
>
>Thanks in advance,
>
>Alberto
>http://www.unmsm.edu.pe/biologia
>Lima PERU
>
>
>
>
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>          ooo0 
>          (    )     0ooo  
>           \  ( ----  (    )--             \_)      )  /
>                      (_/
>__________________________________________________
>Do You Yahoo!?
>Talk to your friends online with Yahoo! Messenger.
>http://messenger.yahoo.com
>---