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From: prismx@scienceweek.com ("Science-Week")
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If you would like to continue to receive announcements of new 
available free ScienceWeek reports, etc., please reply to this 
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If we do not receive a reply from you, we will assume that either 
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From owner-rapd@hgmp.mrc.ac.uk  Wed Jan  5 10:02:59 2000
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From: Mark <stackelberg@mbox.lgm.uni-hannover.de>
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Hi,
I am looking for good online-Graphics of everything that deals with
Molecular Markers. I need it for transparences for lectures about MMs.
Thank you in advance

Mark

    -------------------------
    Mark von Stackelberg
    Lehrgebiet Molekulargenetik
    Universitaet Hannover
    Herrenhaeuser Str. 2
    D-30419 Hannover
    ---------------------------
    Fon : +49 (0)511 7624037
    Fax : +49 (0)511 7624088
    ---------------------------




From owner-rapd@hgmp.mrc.ac.uk  Thu Jan  6 13:23:25 2000
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Subject: Abt: Tons of books!  F.R.E.E...
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---


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From owner-rapd@hgmp.mrc.ac.uk  Fri Jan  7 15:00:54 2000
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From: "Steve Griffiths" <sgriffit@rpc.unb.ca>
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Subject: 3' RACE of bacterial DNA
Date: Fri, 7 Jan 2000 10:59:22 -0400
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Any clues regarding completion of bacterial genes by 3' RACE ?

  Gratitude in advance

  Steve

  sgriffit@rpc.unb.ca




From owner-rapd@hgmp.mrc.ac.uk  Sat Jan  8 06:19:37 2000
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From: Publishing@mx-20.fsnet.co.uk
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Subject: Publishing Company for Sale!
Date: 8 Jan 2000 06:19:31 -0000
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From owner-rapd@hgmp.mrc.ac.uk  Sat Jan  8 07:43:12 2000
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---


From owner-rapd@hgmp.mrc.ac.uk  Wed Jan 12 12:01:00 2000
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*******************************************************
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******************
95037
---


From owner-rapd@hgmp.mrc.ac.uk  Thu Jan 13 09:47:10 2000
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From: Chih-L Han <jesehcl@mac.com>
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Subject: Corbett Research FTS-1 Thermal Sequencer
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Dear News Readers,

Anyone still using using the Corbett Research FTS-1 Thermal Sequencer? Can
you advice where I can get the PCR capillaries and plastic seal plugs?

Thank you very much for your assistance in advance.
          _____
           |_|*     Chih-L Han MD
         {~._.~}       http://macbiomed.cjb.net/
          ( Y )     mailto:jessehcl@cardiologist.org
         ()~*~U)    ICQ:7281420
         (_)-(_)    efax://+1 707 929 0531
            AGCTACGTAAATAATCCTACGGGCACATTGGCAATTGCGTAACG



From owner-rapd@hgmp.mrc.ac.uk  Fri Jan 14 12:18:33 2000
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Subject: Lose 2-14 Inches In ONE Hour! Guaranteed!        (s82nsdk)
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---


From owner-rapd@hgmp.mrc.ac.uk  Fri Jan 14 17:30:33 2000
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Hi All

Being RAPD a dominant marker
I want to know,
How do the RAPD Primers (10mers) flank  only AA y aa
loci in the genome?

I will appreciate any comment

Alberto Donayre
http://www.unmsm.edu.pe/biologia

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From owner-rapd@hgmp.mrc.ac.uk  Sun Jan 16 14:33:15 2000
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From: "Youngmin Park" <bada@shmail.hanarotel.co.kr>
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Subject: Paternity test by PCR?
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  Hello, Scientists.

  I have heard about "Paternity test by PCR". I thought this test is
possible by RFLP.
  If you carry the test by PCR, what is the target gene(or sequence) of the
PCR? Is it a kind of random amplified polymorphic DNA(RAPD) technique?
  Is accuracy of the test by PCR high enough or same with RFLP?

  If you know about this or more... please share your knowledg with me.
Thanks.

-----------------------------
Youngmin Park
bada@shmail.hanarotel.co.kr
011-9890-4270, 032-816-4270
-----------------------------




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From owner-rapd@hgmp.mrc.ac.uk  Tue Jan 18 21:35:12 2000
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From: prakash@Tusk.Edu ("C. S. Prakash")
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Subject: Scientists in Support of Ag Biotechnology: Please help sign the
 petition!!
Date: 18 Jan 2000 21:35:07 -0000
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Dear Scientist Friends:

To promote a responsible use of biotechnology in addressing the 
global problems of agricultural productivity and world hunger, it is 
critical that we as scientists need to be more proactive in making 
our voice heard.  To this end, a petition or declaration has been 
drafted and we need your help.  Please visit the new AgBioWord 
website at http://216.13.247.104/index.html

Read the petition on agricultural biotechnology. If you agree with 
it, please complete the boxes and thus endorse your support for this 
statement.  Eventually, we will submit this statement showing the 
names of all endorsing scientists to various heads of the government, 
international agencies, regulatory agencies, donor organizations, 
media, and other institutions.  Please make this effort a huge 
success and do forward this message to other scientists, scientific 
institutions, and listservs.

I have also included a copy of the petition below.  If you wish, you 
can just email me your intent to support this petition along with 
your name, email address, institution, etc.

Yours,

C. S. Prakash
prakash@tusk.edu
========
Scientists In Support Of Agricultural Biotechnology

  We, the undersigned members of the scientific community, believe 
that recombinant DNA techniques constitute powerful and safe means 
for the modification of organisms and can contribute substantially in 
enhancing quality of life by improving agriculture, health care, and 
the environment.

  The responsible genetic modification of plants is neither new nor 
dangerous. Many characteristics, such as pest and disease resistance, 
have been routinely introduced into crop plants by traditional 
methods of sexual reproduction or cell culture procedures. The 
addition of new or different genes into an organism by recombinant 
DNA techniques does not inherently pose new or heightened risks 
relative to the modification of organisms by more traditional 
methods, and the relative safety of marketed products is further 
ensured by current regulations intended to safeguard the food supply. 
The novel genetic tools offer greater flexibility and precision in 
the modification of crop plants.

  No food products, whether produced with recombinant DNA techniques 
or with more traditional methods, are totally without risk. The risks 
posed by foods are a function of the biological characteristics of 
those foods and the specific genes that have been used, not of the 
processes employed in their development. Our goal as scientists is to 
ensure that any new foods produced from recombinant DNA are as safe 
or safer than foods already being consumed.

  Current methods of regulation and development have worked well. 
Recombinant DNA techniques have already been used to develop 
'environmentally-friendly' crop plants with traits that preserve 
yields and allow farmers to reduce their use of synthetic pesticides 
and herbicides. The next generation of products promises to provide 
even greater benefits to consumers, such as enhanced nutrition, 
healthier oils, enhanced vitamin content, longer shelf life and 
improved medicines.

  Through judicious deployment, biotechnology can also address 
environmental degradation, hunger, and poverty in the developing 
world by providing improved agricultural productivity and greater 
nutritional security. Scientists at the international agricultural 
centers, universities, public research institutions, and elsewhere 
are already experimenting with products intended specifically for use 
in the developing world.

  We hereby express our support for the use of recombinant DNA as a 
potent tool for the achievement of a productive and sustainable 
agricultural system. We also urge policy makers to use sound 
scientific principles in the regulation of products produced with 
recombinant DNA, and to base evaluations of those products upon the 
characteristics of those products, rather than on the processes used 
in their development.


  To add your name to the petition, fill out the form below and return to
mailto:prakash@tusk.edu

  Name:

  Position/Title:

  Organization/Affiliation:

  Department:


  Highest Degree Awarded: Other:


  Discipline:




  The following information is for verification purposes only and will 
not be displayed on the petition.

  E-mail address:
Postal address:
Telephone Number:
---


From owner-rapd@hgmp.mrc.ac.uk  Thu Jan 20 22:12:22 2000
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From: Brian Teasdale <teasdale@cisunix.unh.edu>
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Subject: Microsatellite information
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Dear colleagues,

I am looking for references or people that have done work with haploid
tissue using microsatellites.  I am looking to use develop
microsatellites for an alga that is primarily found in a haploid state.
Any comments on the advantages of using haploid tissue or any haploid
statistical analogs would be greatly appreciated.  Also, does anyone
know of anyone that has tried to use microsatellites in historical
phylogeography.  I am not sure much is known in the rates that
microsatellites evolve and so I would like to know of any thoughts in
this area.   Thank you for your time.

Sincerely,

Brian Teasdale
Univ. of New Hampshire
teasdale@cisunix.unh.edu



From owner-rapd@hgmp.mrc.ac.uk  Thu Jan 20 22:25:57 2000
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From: j3@post.com (J3)
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Subject: Re: Microsatellite information
Date: Thu, 20 Jan 2000 22:25:48 GMT
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I cannot answer to use of haploid tissue for microsatelite analysis.
However, regarding using microsatellites for historical
phylogeography, I would say use it with caution.  Your study areas
should be reasonable in size and you should have known allele
frequencys throughout the study area.  Otherwise, your data could
become meaningless due to the high variance in allele frequencys that
can occur with microsats across large geographic ranges.

Just my $0.02 worth.

Good Day.

<J3>

On Thu, 20 Jan 2000 17:11:59 -0500, Brian Teasdale
<teasdale@cisunix.unh.edu> wrote:

>Dear colleagues,
>
>I am looking for references or people that have done work with haploid
>tissue using microsatellites.  I am looking to use develop
>microsatellites for an alga that is primarily found in a haploid state.
>Any comments on the advantages of using haploid tissue or any haploid
>statistical analogs would be greatly appreciated.  Also, does anyone
>know of anyone that has tried to use microsatellites in historical
>phylogeography.  I am not sure much is known in the rates that
>microsatellites evolve and so I would like to know of any thoughts in
>this area.   Thank you for your time.
>
>Sincerely,
>
>Brian Teasdale
>Univ. of New Hampshire
>teasdale@cisunix.unh.edu
>

________________________________________________

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From owner-rapd@hgmp.mrc.ac.uk  Fri Jan 21 19:41:29 2000
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From: albulb@rocketmail.com (alberto donayre)
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Subject: Taq polimerasa function...
Date: 21 Jan 2000 19:41:25 -0000
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Hi All

Amplication products using 0.5 U of Taq polimerasa in
Oca tubers, are not well defined, Could you recommend
concentrations? Someone recommend me try around 
0.5-3.0 U Could it be possible?

In order to support my experiment, I would like to
know references in testing Taq concentrations and how
its function is performed.

Thanks for  your help in advance,

Alberto Donayre
Universidad San Marcos
http://www.unmsm.edu.pe/biologia

 

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__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
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---


From owner-rapd@hgmp.mrc.ac.uk  Fri Jan 21 21:35:28 2000
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Subject: Re: Taq polimerasa function...
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On 21 Jan 2000 19:41:25 -0000, albulb@rocketmail.com (alberto donayre)
wrote:

>Hi All
>
>Amplication products using 0.5 U of Taq polimerasa in
>Oca tubers, are not well defined, Could you recommend
>concentrations? Someone recommend me try around 
>0.5-3.0 U Could it be possible?
>
>In order to support my experiment, I would like to
>know references in testing Taq concentrations and how
>its function is performed.
>
>Thanks for  your help in advance,
>
>Alberto Donayre
>Universidad San Marcos
>http://www.unmsm.edu.pe/biologia


Have you tried varying the amount of your primer concentration or your
MgCl concentration?  0.5 U of TAQ is planety to drive a PCR reaction!

<J3>
________________________________________________

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Hi:
Instead of sticking to one newsgroup or discussion group,
you can search

for a subject or group from all newsgroups at Altavista.
Use this search
tool at
http://members.xoom.com/ecotao/agric/search.htm

It is a html file, so you can bookmark it, put it in your
start menu,
your desktop or your quicklaunch toolbar! Save it in a
folder on your
computer and use it when you need it. Make a shortcut to
the file and
put it where you need it.

Its really great for News group information.

L.W.E.