From owner-rapd@hgmp.mrc.ac.uk Sat Jun 10 17:14:57 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 3B64E17B37; Sat, 10 Jun 2000 17:14:56 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 1785917ABC; Sat, 10 Jun 2000 17:14:54 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 66A25415DC for ; Sat, 10 Jun 2000 17:14:54 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id RAA17057 for rapd-list@hgmp.mrc.ac.uk; Sat, 10 Jun 2000 17:14:53 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: Tim Fielder X-Newsgroups: bionet.molbio.rapd Subject: Citations Reply-To: tim.fielder@mail.com Message-ID: X-Newsreader: Forte Agent 1.7/32.534 X-No-Archive: yes MIME-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Lines: 18 Date: Sat, 10 Jun 2000 16:14:52 GMT X-Complaints-To: abuse@home.net X-Trace: news1.sttls1.wa.home.com 960653692 24.12.172.181 (Sat, 10 Jun 2000 09:14:52 PDT) Organization: @Home Network To: rapd@hgmp.mrc.ac.uk Sender: owner-rapd@hgmp.mrc.ac.uk Precedence: bulk I am looking for citations for the development of the following techniques (similar to Saiki et al. 1988 for PCR and Williams et al. 1990 for RAPDs). The techniques are the following: - Simple Sequence Repeats (SSR) - Variable Number of Tandem Repeats (VNTR) - Restriction Fragment Length Polymorphism (RFLP) - Protein/Allozyme/Isozyme Electrophoresis Thanks in advance for your help. -Tim Fielder PS: If you use RAPDs much, you should check out this site: http://members.home.net/jwps3/rapd.html From owner-rapd@hgmp.mrc.ac.uk Thu Jun 22 17:45:56 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id DE6C117A8B; Thu, 22 Jun 2000 17:45:55 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 7783517A88; Thu, 22 Jun 2000 17:45:54 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id A2765415D4 for ; Thu, 22 Jun 2000 17:45:53 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id RAA22795 for rapd-list@hgmp.mrc.ac.uk; Thu, 22 Jun 2000 17:45:53 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: cruisefree14@indiatimes.com X-Newsgroups: bionet.molbio.rapd Subject: FREE: 3 Day Trip to Paradise & Deluxe Hotel! Date: 22 Jun 2000 17:45:52 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 24 Message-ID: <20000622164550.99E72415D4@mercury.hgmp.mrc.ac.uk> X-Trace: niobium.hgmp.mrc.ac.uk 961692352 22793 193.62.192.80 (22 Jun 2000 16:45:52 GMT) X-Complaints-To: news@net.bio.net X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 3A5F017A88; Thu, 22 Jun 2000 17:45:51 +0100 (BST) X-Received: from teraa01s.roc-teraa.nl (unknown [195.42.229.226]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 99E72415D4 for ; Thu, 22 Jun 2000 17:45:50 +0100 (BST) X-Received: from 03-200.dial.008.popsite.net by teraa01s.roc-teraa.nl with SMTP (Microsoft Exchange Internet Mail Service Version 5.0.1460.8) id NHNFK12D; Thu, 22 Jun 2000 18:44:27 +0200 X-To: vacationnow9@excite.com To: rapd@hgmp.mrc.ac.uk Sender: owner-rapd@hgmp.mrc.ac.uk Precedence: bulk

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--- From owner-rapd@hgmp.mrc.ac.uk Sat Jun 24 07:00:37 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 7331B17B24; Sat, 24 Jun 2000 07:00:36 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 45F2017B18; Sat, 24 Jun 2000 07:00:35 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id C487B415CF for ; Sat, 24 Jun 2000 07:00:34 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id HAA06529 for rapd-list@hgmp.mrc.ac.uk; Sat, 24 Jun 2000 07:00:34 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: ramen@aau.ren.nic.in (Ramen Sarmah) X-Newsgroups: bionet.molbio.rapd Subject: Band resolution Date: 24 Jun 2000 07:00:32 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 19 Message-ID: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Trace: niobium.hgmp.mrc.ac.uk 961826433 6526 193.62.192.80 (24 Jun 2000 06:00:33 GMT) X-Complaints-To: news@net.bio.net X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 031E117B18; Sat, 24 Jun 2000 07:00:30 +0100 (BST) X-Received: from aau.ren.nic.in (aau.ren.nic.in [164.100.252.82]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id BBB8B415CF for ; Sat, 24 Jun 2000 06:59:58 +0100 (BST) X-Received: from localhost (ramen@localhost) by aau.ren.nic.in (8.8.7/8.8.7) with ESMTP id LAA19638 for ; Sat, 24 Jun 2000 11:51:16 +0530 X-To: rapd@net.bio.net To: rapd@hgmp.mrc.ac.uk Sender: owner-rapd@hgmp.mrc.ac.uk Precedence: bulk I am doing some RAPD work with 10mers random primer. The bannds amplified are very faint after 47 cycles also in 1.5% agarose gel. How to get sharp band? R N Sarma ***************************************************************************** Dr. R. N. Sarma Department of Plant Breeding and Genetics Assam Agricultural University Jorhat- 785 013 Assam India Phone: +91-0376-370928(R) ***************************************************************************** --- From owner-rapd@hgmp.mrc.ac.uk Sun Jun 25 09:08:11 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 822C217AAE; Sun, 25 Jun 2000 09:08:10 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 626F117A6C; Sun, 25 Jun 2000 09:08:09 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id DD04F415D4 for ; Sun, 25 Jun 2000 09:08:08 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id JAA03699 for rapd-list@hgmp.mrc.ac.uk; Sun, 25 Jun 2000 09:08:08 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: qwetasf2390@msn.com X-Newsgroups: bionet.molbio.rapd Subject: Rates Are On The Rise! -msxmvt Date: 25 Jun 2000 09:08:07 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 292 Message-ID: <4vlcpu.5lk72s8o865ekpncf@qrjg2u81.somewhere.com> Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: 8BIT X-Trace: niobium.hgmp.mrc.ac.uk 961920487 3697 193.62.192.80 (25 Jun 2000 08:08:07 GMT) X-Complaints-To: news@net.bio.net X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 7861217A6C; Sun, 25 Jun 2000 09:08:05 +0100 (BST) X-Received: from erin02.phdsystems.com (unknown [206.161.162.177]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id AF30C415D4; Sun, 25 Jun 2000 09:07:57 +0100 (BST) X-Received: from qrjg2u81.somewhere.com ([209.178.152.24]) by erin02.phdsystems.com (Post.Office MTA v3.5 release 215 ID# 0-0U10L2S100) with SMTP id com; Sun, 25 Jun 2000 03:57:01 +0000 X-To: @net.com To: rapd@hgmp.mrc.ac.uk Sender: owner-rapd@hgmp.mrc.ac.uk Precedence: bulk

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Click Here --- From owner-rapd@hgmp.mrc.ac.uk Mon Jun 26 18:10:51 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id B70DB17AAA; Mon, 26 Jun 2000 18:10:50 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 50D0F17A56; Mon, 26 Jun 2000 18:10:49 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id ADA26415DC for ; Mon, 26 Jun 2000 18:10:48 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id SAA06989 for rapd-list@hgmp.mrc.ac.uk; Mon, 26 Jun 2000 18:10:48 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: haashu@Uni-Hohenheim.DE ("H. U. Haas") X-Newsgroups: bionet.molbio.rapd Subject: Re: Band resolution Date: 26 Jun 2000 18:10:47 +0100 Organization: Hohenheim University Lines: 29 Message-ID: <200006261709.TAA24440@ultra3-4.rz.Uni-Hohenheim.DE> References: Mime-Version: 1.0 Content-Type: text/plain; charset=US-ASCII Content-Transfer-Encoding: 7BIT X-Trace: niobium.hgmp.mrc.ac.uk 962039447 6986 193.62.192.80 (26 Jun 2000 17:10:47 GMT) X-Complaints-To: news@net.bio.net X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id BD4C617A56; Mon, 26 Jun 2000 18:10:45 +0100 (BST) X-Received: from ultra3-4.rz.Uni-Hohenheim.DE (ultra3-4.rz.uni-hohenheim.de [144.41.4.28]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 32736415DA for ; Mon, 26 Jun 2000 18:10:40 +0100 (BST) X-Received: from herbo8x ([144.41.144.69]) by ultra3-4.rz.Uni-Hohenheim.DE (8.8.3/8.8.3) with ESMTP id TAA24440; Mon, 26 Jun 2000 19:09:47 +0200 (MET DST) X-To: ramen@aau.ren.nic.in (Ramen Sarmah) X-Cc: rapd@net.bio.net X-Return-Receipt-To: "H. U. Haas" X-Priority: normal X-In-reply-to: X-Mailer: Pegasus Mail for Win32 (v3.12b) To: rapd@hgmp.mrc.ac.uk Sender: owner-rapd@hgmp.mrc.ac.uk Precedence: bulk Dear Colleague, We are doing similar work with weeds. 45 cycles, 10mer primers, 1.5 % agarose gel. Half a year ago one of our master students tested different Taq and had different results. Maybe you also have problems with the control? Then you should try a different Taq- Polymerase provider. We got best results with the Taq DNA Polymerase provided by Roche Diagnostics. I know it is expensive, but it works. Wish you good success. Best regards Hans Ulrich Haas ---------------------------------------------------- ----------------------------------------------------- Dr. Hans Ulrich Haas Hohenheim University Institute of Phytomedicine (360) D-70593 Stuttgart Germany Fax: +49 711 4592408 E-mail: haashu@uni-hohenheim.de ------------------------------- --- From owner-rapd@hgmp.mrc.ac.uk Mon Jun 26 18:28:20 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 02E5117AAA; Mon, 26 Jun 2000 18:28:19 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id C3B6417AB7; Mon, 26 Jun 2000 18:28:18 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 26FBB415DC for ; Mon, 26 Jun 2000 18:28:18 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id SAA07221 for rapd-list@hgmp.mrc.ac.uk; Mon, 26 Jun 2000 18:28:17 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: bbolivaljr@mailcity.com ("Benjamin, Jr. Bolival") X-Newsgroups: bionet.molbio.rapd Subject: Re: Band resolution Date: 26 Jun 2000 18:28:16 +0100 Organization: MailCity (http://www.mailcity.lycos.com:80) Lines: 15 Message-ID: Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Trace: niobium.hgmp.mrc.ac.uk 962040497 7218 193.62.192.80 (26 Jun 2000 17:28:17 GMT) X-Complaints-To: news@net.bio.net Content-Language: en X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 6030717AAA; Mon, 26 Jun 2000 18:28:15 +0100 (BST) X-Received: from mailcity.com (fes-qout.whowhere.com [209.1.236.7]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id AD5AC415DC for ; Mon, 26 Jun 2000 18:28:13 +0100 (BST) X-Received: from Unknown/Local ([?.?.?.?]) by mailcity.com; Mon Jun 26 10:27:41 2000 X-To: rapd@hgmp.mrc.ac.uk, "" H.U.Haas "" X-Sent-Mail: off X-Reply-To: bbolivaljr@mailcity.com X-Mailer: MailCity Service X-Sender-Ip: 208.142.145.36 To: rapd@hgmp.mrc.ac.uk Sender: owner-rapd@hgmp.mrc.ac.uk Precedence: bulk Hello all, In my experience in Musa spp.(bananas), Taq Polymerase from Life Technologies/Gibco worked well at 45 C, 10mers, 45 cycles, 1.5 MgCl2..... Benjamin J. Bolival, Jr. Natural Sciences Research Institute University of the Philippines Diliman, Quezon City, Philippines Send FREE Greetings for Father's Day--or any day! Click here: http://www.whowhere.lycos.com/redirects/fathers_day.rdct --- From owner-rapd@hgmp.mrc.ac.uk Wed Jun 28 10:26:22 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 49F8B17AE5; Wed, 28 Jun 2000 10:26:21 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 19A0117A7F; Wed, 28 Jun 2000 10:26:20 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 999C1415DD for ; Wed, 28 Jun 2000 10:26:19 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id KAA23290 for rapd-list@hgmp.mrc.ac.uk; Wed, 28 Jun 2000 10:26:19 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: ramen@aau.ren.nic.in (Ramen Sarmah) X-Newsgroups: bionet.molbio.rapd Subject: Band resolution Date: 28 Jun 2000 10:26:17 +0100 Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Lines: 19 Message-ID: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Trace: niobium.hgmp.mrc.ac.uk 962184378 23288 193.62.192.80 (28 Jun 2000 09:26:18 GMT) X-Complaints-To: news@net.bio.net X-Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 7402B17A7F; Wed, 28 Jun 2000 10:26:15 +0100 (BST) X-Received: from aau.ren.nic.in (aau.ren.nic.in [164.100.252.82]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 28914415DC for ; Wed, 28 Jun 2000 10:25:18 +0100 (BST) X-Received: from localhost (ramen@localhost) by aau.ren.nic.in (8.8.7/8.8.7) with ESMTP id PAA31337 for ; Wed, 28 Jun 2000 15:15:53 +0530 X-To: rapd@net.bio.net To: rapd@hgmp.mrc.ac.uk Sender: owner-rapd@hgmp.mrc.ac.uk Precedence: bulk Dear colleague, Thank you for your mails after my posting to the discussion group. Now I am getting good bands with 2.0% agarose gel. ***************************************************************************** Dr. R. N. Sarma Associate Professor Department of Plant Breeding and Genetics Assam Agricultural University Jorhat- 785 013 Assam India Phone: +91-0376-370928(R) ***************************************************************************** --- From owner-rapd@hgmp.mrc.ac.uk Fri Jun 30 20:29:09 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id D04A617A67; Fri, 30 Jun 2000 20:29:06 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 6785617A67; Fri, 30 Jun 2000 20:29:03 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9EBAC415D5; Fri, 30 Jun 2000 20:29:02 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id UAA23308; Fri, 30 Jun 2000 20:29:01 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: "Music Sender" X-Newsgroups: bionet.molbio.rapd,bionet.xtallography,bit.listserv.geograph,borland.public.cppbuilder.graphics,borland.public.delphi.graphics,cl.magazine.gegenstandpunkt,comp.graphics.algorithms,comp.graphics.animation,comp.graphics.api.inventor,comp.graphics.ap��V Subject: Who needs a VCR when you have SX! 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