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From: patman_c@my-deja.com
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Subject: How does RAPD work?
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  Hi,
I'm a genetics major at Texas A&M.  The other day I attended a lecture
on genome sequencing.  The professor mentioned a technique called RAPD,
but did not go into detail on it.  I've searched around trying to find
out about it  but all I seem to find is technical information on it.  I
was wondering if anyone could explain this process to me in more
layman's terms?  Thanks,
Patrick


Sent via Deja.com http://www.deja.com/
Before you buy.




From owner-rapd@hgmp.mrc.ac.uk  Thu Nov  2 15:40:18 2000
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From: ajdt1@hotmail.com ("ALBERTO DONAYRE")
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Subject: RE: How does RAPD work?
Date: 2 Nov 2000 15:39:57 -0000
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HI
RAPD IS A VARIATION OF THE CONVENTIONAL PCR AMPLIFICATION THAT USES A 
THERMAL ENZYME TO AMPLIFY SEGMENTS IN THE TARGET GENOME, THE CONVENTIONAL 
PCR USES A FORWARD AND REWARD PRIMERS NOT RANDOM. RAPD USES RANDOM PRIMERS, 
THIS MEAN THAT YOU WILL DO NOT KNOW THE SECUENCE OF THE RESULTING DNA 
FRAGMENT.

IF TWO RANDOM PRIMERS FIND EACH OTHER IN AN  OPPOSITE DNA STRAND AND IN 
AMPLIFICABLE DISTANCE THEY PRODUCE A FRAGMENT WHICH REPRESENTS A RANDOM LOCI 
IN THE TARGET GENOME.

MANY BREEDERS HAVE NOTICE THAT RAPD ONLY DETECTS THE -AA- AND -aa- LOCI IN 
THE GENOME, SO THEY ARE CALLED AS DOMINANT MARKERS, THEY DO NOT SHOW ANY 
-Aa- LOCI.

WHY? I WOULD LIKE TO KNOW, I ASKED MANY PEOPLE BUT NOBODY GAVE ME AN 
APPROPIATE ANSWER

HOPE THIS HELPS

ALBERTO DONAYRE
LIMA-PERU
_________________________________________________________________________
Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com.

Share information about yourself, create your own public profile at 
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From owner-rapd@hgmp.mrc.ac.uk  Fri Nov  3 01:34:40 2000
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From: orban@ima.org.sg (Laszlo Orban)
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Subject: RE: How does RAPD work?
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Dear Alberto,


>MANY BREEDERS HAVE NOTICE THAT RAPD ONLY DETECTS THE -AA- AND -aa- LOCI IN 
>THE GENOME, SO THEY ARE CALLED AS DOMINANT MARKERS, THEY DO NOT SHOW ANY
-Aa- LOCI.
>WHY? I WOULD LIKE TO KNOW, I ASKED MANY PEOPLE BUT NOBODY GAVE ME AN
APPROPIATE ANSWER
This is a misunderstanding. RAPD DOES detect the heterozygotes (Aa or +/-),
except that it is not possible to differentiate them from the homozygous
(AA or +/+) genotypes, since the band in question is amplified from both.
(Earlier people tried to sort them on the basis of band intensity, but this
approach did not work.)

For this reason both +/+ and +/- give you a band (dominant) and only -/-
doesn't (recessive). This is why RAPDs are called dominant markers, since
the heterozygotes could only be detected by crossing them with a recessive
homozygote (-/-) and then assaying their F1s. All F1s with the selected
band in the given RAPD assay would indicate a homozygote (+/+) parent,
while 50% would point to a heterozygote (+/-).

There are a few codominant RAPD described in the literature. They might be
caused by a short deletion or insertion in between two suitably located
primer binding sites. In that case the recessive allele appears in the
pattern and its connection to the dominant one could be observed. Most of
the RAPD markers (>95% or more) are however dominant as you noted correctly
in your message.

Cheers: L.
<|||>< <|||>< <|||>< <|||>< <|||>< <|||>< <|||><

Laszlo Orban, Ph.D.  
Senior Scientist
Laboratory of Fish Biotechnology
Institute of Molecular Agrobiology
National University of Singapore
1 Research Link, NUS Campus
Singapore 117 604

Tel: 65-872-7413
Fax: 65-872-7007
E-mail: orban@ima.org.sg
Web: http://research.ima.org.sg/research/fish/orban/

><III> ><III> ><III> ><III> ><III> ><III> ><III> 



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From owner-rapd@hgmp.mrc.ac.uk  Sat Nov 18 22:41:46 2000
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patman_c@my-deja.com wrote:

>   Hi,
> I'm a genetics major at Texas A&M.  The other day I attended a lecture
> on genome sequencing.  The professor mentioned a technique called RAPD,
> but did not go into detail on it.  I've searched around trying to find
> out about it  but all I seem to find is technical information on it.  I
> was wondering if anyone could explain this process to me in more
> layman's terms?  Thanks,
> Patrick
>
> Sent via Deja.com http://www.deja.com/
> Before you buy.




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From owner-rapd@hgmp.mrc.ac.uk  Wed Nov 22 19:06:06 2000
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From: lkspft@hotmail.com
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Subject: The Perfect Christmas Gift  8159
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Two days ago I ordered 10 pieces of paper money from 10 different countries from a company called Perth Numismatics. Lo and behold they arrived today and they are very nice and colourful. They even have a bill from Antarctica. I didn't even know they existed. These are perfect for stocking stuffers or people who are hard to buy for. The website address is www.perthmoney.com
Good luck and Merry Christmas

Cynthia Reeves

kdhjeutlmfrvhyyjqgloojxrtgxyfnocgjibngvesgbechgzlteijpgrt






From owner-rapd@hgmp.mrc.ac.uk  Mon Nov 27 23:33:09 2000
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From owner-rapd@hgmp.mrc.ac.uk  Tue Nov 28 13:19:22 2000
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Subject: Be You Own Boss. Work at Home & Make Great Money!                         6190
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Tired of the 40 X 40 X 40 Plan? You know: 

Work 40 hours per week for someone else for 40 years, then receive a 40% reduction in pay! 

Is working for a "boss" too demeaning and unrewarding? 

Are you sick of depending on a job with too little pay and too many hours with no personal reward and even less future?

If you're determined to retire in the next 2 - 5 years with enough income to have REAL Financial Independence and Freedom, and are not afraid to work for it, I can help you.

I am looking for people with a Good Work Ethic and extraordinary Desire to Earn at Least $10,000 per Month Working From Home!

NO SPECIAL SKILLS OR EXPERIENCE REQUIRED. We will give you all the training and personal support you will need to ensure your success!

This LEGITIMATE HOME-BASED INCOME OPPORTUNITY can put you back in Control of your Time, Your Finances, and Your Life!

If you've tried other opportunities in the past that have failed to live up to their promises, THIS IS DIFFERENT THAN ANYTHING ELSE YOU'VE SEEN!

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YOUR FINANCIAL PAST DOES NOT HAVE TO BE YOUR FINANCIAL FUTURE!

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1-800-533-9351 (Free, 24 hour, 2 minute recorded message)

DON'T GO TO SLEEP WITHOUT LISTENING TO THIS!

"All our dreams can come true - if we have the courage to pursue them" - Walt Disney



To be removed from future mailings, send an email to:  nht5thn3@yahoo.com and type "Remove" in the subject line.


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From owner-rapd@hgmp.mrc.ac.uk  Thu Nov 30 00:55:58 2000
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From: mnadiv@barak-online.net (The Medical Malpractice Victims Organization - Shaare Zedek Israel)
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Subject: WARNING: Prof. Arnon Samueloff M.D. - WARNING: Medical Malpractice - Israel
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WARNING: Prof. Arnon Samueloff MD - WARNING: Medical Malpractice in Shaare Zedek Hospital
 
http://www.szmc.co.il/samueloff/
http://www.szmc.co.il/mal/index.html
 
Read More in the Medical Malpractice Victims Organization - Shaare Zedek Israel site.


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