From owner-recombination@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!rutgers!csn!carbon!night.primate.wisc.edu!sdd.hp.com!swrinde!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: matrixwho@aol.com (Matrix Who) Newsgroups: bionet.molbio.recombination Subject: Are there any really interesting things being done with recombinant DNA? Date: 3 Dec 1995 21:31:50 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 2 Sender: root@newsbf02.news.aol.com Message-ID: <49tmim$2t4@newsbf02.news.aol.com> Reply-To: matrixwho@aol.com (Matrix Who) NNTP-Posting-Host: newsbf02.mail.aol.com Are there any really interesting things being done with recombinant DNA? I f there is , what is it? What is being done with recombinant DNA so far? From owner-recombination@net.bio.net Sun Dec 03 22:00:00 1995 Path: biosci!ATLAS.ODYSSEE.NET!dellaire From: dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: RE: Are there any really interesting things being done with recombinant DNA? YOU BET THERE ARE : ) Date: 4 Dec 1995 10:06:41 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 56 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BAC248.27455900@pool7_4.odyssee.net> NNTP-Posting-Host: net.bio.net ---------- From: Matrix Who[SMTP:matrixwho@aol.com] Sent: Sunday, December 03, 1995 9:31 PM To: recom@net.bio.net Subject: Are there any really interesting things being done with = recombinant DNA? Are there any really interesting things being done with recombinant DNA? = I f there is , what is it? What is being done with recombinant DNA so far? Hmmm very open ended indeed. But just the thing to get a thread = started! One thing I think that is very interesting with recombinant DNA as of = late 1. Creation of drug resistant population of bone marrow cells (ex vivo = gene therapy) to help repopulate the bone marrow of leukemia patients = after an extreme chemotherapy regime (ie. transfer of glutathione S-transferase Yc gene) 2. The plethora of "immunity from within" trials going on with studies = on HIV-1 and 2. =20 ex. expression of protease constitutively in CD-34 + T-cells which = prevents the normal maturation of the virus. (gag-pol needs to be = cleaved specifically at the time of budding I believe) 3. The use of site specific recombination systems to create Transgenic = mice with conditional knockouts for genes that as nulls are lethal in = development. For example flanking a gene with loxp sites (targets for = Cre recombinase from P-phage) in direct orientation. Then you cross = this mouse with one carrying Cre recombinase with an inducible promoter. = Result hybrid mice that once fully developed can have the gene knocked = out by the induction of Cre recombinase. That should help to get the ball rolling Graham=20 Primary Discussion Leader RECOM dellaire@odyssee.net Exp. Medicine McGill University From owner-recombination@net.bio.net Mon Dec 04 22:00:00 1995 Path: biosci!agate!dog.ee.lbl.gov!news.cs.utah.edu!cs.utexas.edu!swrinde!newsfeed.internetmci.com!in2.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: Conference in DNA Replication Date: 4 Dec 1995 23:20:54 GMT Organization: McGill University Lines: 65 Message-ID: <49vvom$53i@sifon.cc.mcgill.ca> NNTP-Posting-Host: g-02.das.mcgill.ca X-Newsreader: SPRY News 3.03 (SPRY, Inc.) 2nd Announcement The Fourth McGill University Conference on Regulation of Eukaryotic DNA Replication St. Sauveur, Quebec, Canada October 1996. Organized by: Maria Zannis-Hadjopoulos and Gerald Price McGill Cancer Centre, Department of Oncology Faculty of Medicine and Faculty of Graduate Studies and Research McGill University http://www.mcgill.ca/mcgill/servers/Admin/UBO/dna.html The meeting will include plenary lecture sessions and poster sessions. TOPICS: * Eukaryotic Origins of DNA Replication * Nuclear and DNA Structure in DNA Replication * Modulation of Eukaryotic DNA Replication Preliminary Program The conference will be held at the Manoir Saint-Sauveur in the Laurentians. The first session will begin in the evening of October 17 and the final session will end in the early afternoon of October 20. Poster and oral presentations will take place on October 18 and 19. All participants are encouraged to present a poster on any of the topics that will be covered in the conference. Conference Location: The meeting will be held at the Laurentians' newest four-season conference resort hotel - a major luxury complex just about 45 minutes from Montreal, amid the dynamic beauty of the Laurentians. Saint-Sauveur-des-Monts, the Laurentians' most picturesque village, is the gateway village to the Laurentians. Transportation will be available from the airport to the conference site, and return. Registration materials and information regarding abstract submission will be sent in early 1996. *********Abstract deadline date: June 27, 1996.************ For More Information Please visit our Web Site http://www.mcgill.ca/mcgill/servers/Admin/UBO/dna.html From this site you can obtain: * A Preliminary Program * Further Information on Conference Location * A Reply Card to Obtain Registration Package G. Dellaire Exp. Medicine McGill University dellaire@odyssee.net From owner-recombination@net.bio.net Mon Dec 04 22:00:00 1995 Path: biosci!agate!news.Stanford.EDU!nntp-hub2.barrnet.net!newsfeed.internetmci.com!howland.reston.ans.net!news-e1a.megaweb.com!newstf01.news.aol.com!newsbf02.news.aol.com!not-for-mail From: matrixwho@aol.com (Matrix Who) Newsgroups: bionet.molbio.recombination Subject: Is there anything being done with Spina Bifida using recombinant DNA techniques? Date: 4 Dec 1995 16:16:53 -0500 Organization: America Online, Inc. (1-800-827-6364) Lines: 3 Sender: root@newsbf02.news.aol.com Message-ID: <49vog5$p7s@newsbf02.news.aol.com> Reply-To: matrixwho@aol.com (Matrix Who) NNTP-Posting-Host: newsbf02.mail.aol.com Is there anything being done with Spina Bifida using recombinant DNA techniques? I heard there was and if anyone could update me on this topic , it would be gratefully aprreciated. From owner-recombination@net.bio.net Mon Dec 04 22:00:00 1995 Path: biosci!agate!news.Stanford.EDU!nntp-hub2.barrnet.net!newsfeed.internetmci.com!in2.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: RE: Are there any interesting Developments in recombinant DNA Date: 4 Dec 1995 23:34:39 GMT Organization: McGill University Lines: 47 Message-ID: <4a00if$53i@sifon.cc.mcgill.ca> NNTP-Posting-Host: g-02.das.mcgill.ca X-Newsreader: SPRY News 3.03 (SPRY, Inc.) ---------- From: Matrix Who[SMTP:matrixwho@aol.com] Sent: Sunday, December 03, 1995 9:31 PM To: recom@net.bio.net Subject: Are there any really interesting things being done with recombinant DNA? Are there any really interesting things being done with recombinant DNA? I f there is , what is it? What is being done with recombinant DNA so far? Hmmm very open ended indeed. But just the thing to get a thread started! One thing I think that is very interesting with recombinant DNA as of late 1. Creation of drug resistant population of bone marrow cells (ex vivo gene therapy) to help repopulate the bone marrow of leukemia patients after an extreme chemotherapy regime (ie. transfer of glutathione S-transferase Yc gene) 2. The plethora of "immunity from within" trials going on with studies on HIV-1 and 2. ex. expression of protease constitutively in CD-34 + T-cells which prevents the normal maturation of the virus. (gag-pol needs to be cleaved specifically at the time of budding I believe) 3. The use of site specific recombination systems to create Transgenic mice with conditional knockouts for genes that as nulls are lethal in development. For example flanking a gene with loxp sites (targets for Cre recombinase from P-phage) in direct orientation. Then you cross this mouse with one carrying Cre recombinase with an inducible promoter. Result hybrid mice that once fully developed can have the gene knocked out by the induction of Cre recombinase. That should help to get the ball rolling Graham Primary Discussion Leader RECOM dellaire@odyssee.net Exp. Medicine McGill University From owner-recombination@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!ATLAS.ODYSSEE.NET!dellaire From: dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: RE: (none) Date: 6 Dec 1995 12:21:49 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 80 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BAC3ED.547DB560@pool2_4.odyssee.net> NNTP-Posting-Host: net.bio.net Date: Tue, 05 Dec 1995 15:28:25 -0600 From: Bob Lansman (by way of Bob Lansman ) In response to the "anything interesting in recombinant DNA" thread, = G.D. mentioned systems in which tissue-specific conditional knockouts could = be produced. I'm extremely interested in this possibility. Can anyone = steer me to a reference or two to get me started. Here are two recent references =20 Title: Inducible gene targeting in mice. Source: Science. 269(5229):1427-9, 1995 Sep 8. =20 Authors: Kuhn R. Schwenk F. Aguet M. Rajewsky K. =20 Title: A site-directed chromosomal translocation induced in embryonic = stem cells =20 by Cre-loxP recombination. =20 Source: Nature Genetics. 9(4):376-85, 1995 Apr.=20 Authors:Smith AJ. De Sousa MA. Kwabi-Addo B. Heppell-Parton A. = Impey H. =20 Rabbitts P. =20 A second and completely unrelated question. A couple of weeks ago, just = as the Recom list was being formed, I asked whether anyone knows how DNA = cross links, such as the photoproduct of psoralen, are repaired. No one = answered so I'm asking again. Thanks. Bob Lansman Here is a reference I hope it helps... DNA repair is not my forte. I am = looking for someone strong in DNA to be an associated discussion leader. = If anyone you know is interested tell them to apply by writing me at=20 dellaire@odyssee.net Minimal requirement is access to e-mail and a little ethusiasm about = science. Title: Altered repair of targeted psoralen photoadducts in the = context of an =B3 oligonucleotide-mediated triple helix. = =B3 Source: Journal of Biological Chemistry. 270(38):22595-601, 1995 Sep = 22.=20 Authors: Wang G. Glazer PM. = =20 Graham Dellaire Discussion Leader RECOM Exp Medicine McGill P.S. How about a little help people... Rome was not built by one man and = neither was a successfull news group. If you don't help others why = should they help you. something to think about . Cheers all who actually participate rather than haunt bionet. From owner-recombination@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!ATLAS.ODYSSEE.NET!dellaire From: dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: Lorne Genome Conference in DNA Recombination and Repair Date: 6 Dec 1995 13:04:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 73 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BAC3F3.4B113C80@pool2_4.odyssee.net> NNTP-Posting-Host: net.bio.net This posting was unsolicited ... I found it in a routine Web search and I thought many more people would be interested. Graham RECOM An invitation to attend the 1996 Lorne Genome Conference The Lorne Genome Conference will be held at Erskine House from 12 - 16 February 1996. Details of organising committee Symposia Topics and Speakers RECOMBINATION AND REPAIR Art Landy, Division of Biology and Medicine, Brown University, Rhode Island, USA. Miroslav Radman, Laboratoire de Mutagenese, Institut J. Monod, Paris, France. Overiview Recombination and repair are enjoying renewed interest because of the role of site-specific recombination in gene targeting and the sequence homology of human cancer genes with DNA repair genes. *Art Landy, Division of Biology and Medicine, Brown University, Rhode Island, USA. Professor Landy studied for his PhD degree with Sol Spiegelman followed by a postdoctoral fellowship with Sydney Brenner and Francis Crick. After continuing his studies of the synthesis and structure of tRNA for several years he initiated studies into viral integration. Over the last 20 years he has produced a steady stream of publications which now provide us with a sophisticated understanding of the molecular mechanism of integration and excision of prophage lambda. His review of Int and FLP in Current Opinion in Genetics and Development, 3, 699-707, 1993 indicates why he is now internationally identified with the field of site-specific recombination. *Miroslav Radman, Laboratoire de Mutagenese, Institut J. Monod, Paris, France. Professor Radman received a PhD from the Free University of Brussels where he was subsequently a Faculty member for 10 years. During this period he had several periods as a visiting Professor in Matthew Messelson's laboratory at Harvard before he joined CNRS as Director in 1983. He and Messelson discovered the role of methylation in mismatch repair and he was the first to propose the SOS hypothesis to explain the origins of inducible mutations in bacteria. He has made numerous other contributions to the understanding of the role of DNA repair mechanisms in mutagenesis in organisms ranging from bacteria to humans. He is internationally noted as an exciting and fascinating speaker who has the ability to make complex subjects appear logical and straightforward. He is also noted for his well-supported arguments which show the important part played by studies in bacteria in the development of our understanding of a wide range of biological phenomena, including and especially cancer and aging. Professor Radman is fluent in several languages and is reported to be a dynamic conversationalist in all of them! Address for correspondence: Jeremy Timmis Department of Genetics, University of Adelaide, Adelaide 5005 Tel (08) 303 4661 FAX (08) 303 4399 Email jtimmis@genetics.adelaide.edu.au More Info on other Conferences at http://grimwade.biochem.unimelb.edu.au/lorne/genome.htm From owner-recombination@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!bdg10.niddk.nih.gov!KAYUWEW From: KAYUWEW@bdg10.niddk.nih.gov ("WAGNER, KAY-UWE") Newsgroups: bionet.molbio.recombination Subject: Floxed Mice? Date: 6 Dec 1995 12:25:42 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <30C5FBE9@SMTP2.mm.hub.nih.gov> NNTP-Posting-Host: net.bio.net Does anybody have floxed mice (endog. or transg.) to test my Wap-Cre transgenic mice (Cre expression in epithel. cells of mammary gland) ? kayuwew@bdg10.niddk.nih.gov From owner-recombination@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!ATLAS.ODYSSEE.NET!dellaire From: dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: RE: Site Specific Reference for Bob Lansman and Psoralen Products TOO! Date: 6 Dec 1995 12:25:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 83 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BAC3ED.CCE5FD00@pool2_4.odyssee.net> NNTP-Posting-Host: net.bio.net I am sending this again so I hope you see it BOB.... Crazy things a foot in cyberspace Date: Tue, 05 Dec 1995 15:28:25 -0600 From: Bob Lansman (by way of Bob Lansman ) In response to the "anything interesting in recombinant DNA" thread, = G.D. mentioned systems in which tissue-specific conditional knockouts could = be produced. I'm extremely interested in this possibility. Can anyone = steer me to a reference or two to get me started. Here are two recent references =20 Title: Inducible gene targeting in mice. Source: Science. 269(5229):1427-9, 1995 Sep 8. =20 Authors: Kuhn R. Schwenk F. Aguet M. Rajewsky K. =20 Title: A site-directed chromosomal translocation induced in embryonic = stem cells =20 by Cre-loxP recombination. =20 Source: Nature Genetics. 9(4):376-85, 1995 Apr.=20 Authors:Smith AJ. De Sousa MA. Kwabi-Addo B. Heppell-Parton A. = Impey H. =20 Rabbitts P. =20 A second and completely unrelated question. A couple of weeks ago, just = as the Recom list was being formed, I asked whether anyone knows how DNA = cross links, such as the photoproduct of psoralen, are repaired. No one = answered so I'm asking again. Thanks. Bob Lansman Here is a reference I hope it helps... DNA repair is not my forte. I am = looking for someone strong in DNA to be an associated discussion leader. = If anyone you know is interested tell them to apply by writing me at=20 dellaire@odyssee.net Minimal requirement is access to e-mail and a little ethusiasm about = science. Title: Altered repair of targeted psoralen photoadducts in the = context of an =B3 oligonucleotide-mediated triple helix. = =B3 Source: Journal of Biological Chemistry. 270(38):22595-601, 1995 Sep = 22.=20 Authors: Wang G. Glazer PM. = =20 Graham Dellaire Discussion Leader RECOM Exp Medicine McGill P.S. How about a little help people... Rome was not built by one man and = neither was a successfull news group. If you don't help others why = should they help you. something to think about . Cheers all who actually participate rather than haunt bionet. From owner-recombination@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!PO-BOX.MCGILL.CA!popa0206 From: popa0206@PO-BOX.MCGILL.CA Newsgroups: bionet.molbio.recombination Subject: Re:COns of Recombinant Tech and Food Crops Date: 6 Dec 1995 11:43:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512061941.OAA28607@sirocco.CC.McGill.CA> References: NNTP-Posting-Host: net.bio.net On Tue, 5 Dec 1995, Ian A Finlay wrote: >Hello-my name is Ian Finlay and I'm doing a presentation on the cons of >genetic recombination of plants for increased yields for crops-can you >please give me a minute or two and give me some insight on some of the >negative aspects of recombination?! >Thank you very much, >Sincerely, >Ian Finlay > > > > > Sure Ian I would be happy to help... Cons of recombinant technology in regard to plant genetics and increased crop yields. 1. Until recently there was no way to remove the antibiotic resistance gene that was used to mark the "new" gene when it was transfered to the plants genome. This means for instance that you might have an allergy to that particular antibiotic resistance protein (say neomycin) and eating such an engineered plant might actually harm you. Also as more and more engineered plants come to market you could have a smorgasboard of proteins and recombinant dna in your diet which encourage antibiotic resistant bacteria to develop in our population. Bacteria are fairly premiscuous when it comes to DNA and they can incorporate those "marker" genes and become themselves resistant to antibiotics. The main problem is allergies not resistant bacteria as the wide spread over-use of antibiotics world wide has made this a mute point. Fortunately, the next generation of engineered plants should be able to make use of site specific recombination technology (ask Dr. Szatsmari : ) ) which can remove the antibiotic "marker" before you ever have the displeasure of eating a tomato laced with a potential allergen. Graham Dellaire Exp. Medicine McGill University Montreal, Quebec From owner-recombination@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!ATLAS.ODYSSEE.NET!dellaire From: dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: Request on Site specific Recombination by Bob Lansman Date: 6 Dec 1995 12:00:31 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 30 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BAC3EA.5CDD98E0@pool2_4.odyssee.net> NNTP-Posting-Host: net.bio.net ---------- From: PMDF Mail Server, by way of Bob Lansman [SMTP:postmaster@VAXA.CIS.UWOSH.EDU] Sent: Wednesday, December 06, 1995 8:36 AM To: recom@net.bio.net Subject: (none) Date: Tue, 05 Dec 1995 15:28:25 -0600 From: Bob Lansman In response to the "anything interesting in recombinant DNA" thread, G.D. mentioned systems in which tissue-specific conditional knockouts could be produced. I'm extremely interested in this possibility. Can anyone steer me to a reference or two to get me started. A second and completely unrelated question. A couple of weeks ago, just as the Recom list was being formed, I asked whether anyone knows how DNA cross links, such as the photoproduct of psoralen, are repaired. No one answered so I'm asking again. Thanks. Bob Lansman From owner-recombination@net.bio.net Tue Dec 05 22:00:00 1995 Path: biosci!VAXA.CIS.UWOSH.EDU!postmaster From: postmaster@VAXA.CIS.UWOSH.EDU (PMDF Mail Server, by way of Bob Lansman ) Newsgroups: bionet.molbio.recombination Subject: (none) Date: 6 Dec 1995 05:36:30 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <01HYH9BJB136003I94@VAXA.CIS.UWOSH.EDU> NNTP-Posting-Host: net.bio.net The message could not be delivered to: Date: Tue, 05 Dec 1995 15:28:25 -0600 From: Bob Lansman (by way of Bob Lansman ) Subject: conditional knockouts X-Sender: lansman@vaxa.cis.uwosh.edu To: recomb@net.bio.net Message-id: <01HYGBNTRGC20034EB@VAXA.CIS.UWOSH.EDU> MIME-version: 1.0 X-Mailer: Windows Eudora Version 2.1.1 Content-type: text/plain; charset=us-ascii Content-transfer-encoding: 7BIT In response to the "anything interesting in recombinant DNA" thread, G.D. mentioned systems in which tissue-specific conditional knockouts could be produced. I'm extremely interested in this possibility. Can anyone steer me to a reference or two to get me started. A second and completely unrelated question. A couple of weeks ago, just as the Recom list was being formed, I asked whether anyone knows how DNA cross links, such as the photoproduct of psoralen, are repaired. No one answered so I'm asking again. Thanks. Bob Lansman From owner-recombination@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!agate!news.Stanford.EDU!nntp-hub2.barrnet.net!news1.digital.com!uunet!in2.uu.net!newsflash.concordia.ca!feed.umontreal.ca!szat From: szat@ERE.UMontreal.CA (Szatmari George) Newsgroups: bionet.molbio.recombination Subject: repair of DNA cross links Date: 6 Dec 1995 20:16:34 GMT Organization: Universite de Montreal Lines: 18 Distribution: world Message-ID: <4a4tn2$9qj@epervier.CC.UMontreal.CA> NNTP-Posting-Host: alize.ere.umontreal.ca Hi folks, I just had a message forwarded to me asking about the repair of DNA crosslinks, such as those made by psoralen. In the case of Ecoli, it has been proposed that the products of the uvrABC genes form the UvrABC excinuclease, which cleaves the DNA single strand first on the 5' end of the crosslink, followed by a 3' cleavage generating a single stranded gap (which could be up to 1000 nt), which is then repaired by recA recombination. This is taken from E coli and S typhimurium, F Neidhardt, ed. 1987. I can't really say what happens in eukaryotes - any takers? George Szatmari ps there hasn't been much posted here lately, at least over here. Is this true everywhere else? From owner-recombination@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!GPU.SRV.UALBERTA.CA!hastings From: hastings@GPU.SRV.UALBERTA.CA (P Hastings) Newsgroups: bionet.molbio.recombination Subject: Re: repair of DNA cross links Date: 7 Dec 1995 07:50:14 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <4a4tn2$9qj@epervier.CC.UMontreal.CA> NNTP-Posting-Host: net.bio.net The answer to Lansman's question in repair of psoralen crosslinks in yeast is given by: Jachymczyk et al. Mol. gen. Genetics 182:196-205. 1981. What we found was that the RAD3 system excises the crosslink on both strands to make a double strand break, which is then repaired by RAD52 dependent recombination. Phil Hastings. On 6 Dec 1995, Szatmari George wrote: > Hi folks, > > I just had a message forwarded to me asking about the repair > of DNA crosslinks, such as those made by psoralen. In the case > of Ecoli, it has been proposed that the products of the uvrABC > genes form the UvrABC excinuclease, which cleaves the DNA single > strand first on the 5' end of the crosslink, followed by a 3' cleavage > generating a single stranded gap (which could be up to 1000 nt), which > is then repaired by recA recombination. This is taken from E coli and > S typhimurium, F Neidhardt, ed. 1987. > > I can't really say what happens in eukaryotes - any takers? > > George Szatmari > > ps there hasn't been much posted here lately, at least over here. Is > this true everywhere else? > > > From owner-recombination@net.bio.net Wed Dec 06 22:00:00 1995 Path: biosci!cifn.unam.mx!edgar From: edgar@cifn.unam.mx (Edgar Valencia) Newsgroups: bionet.molbio.recombination Subject: Re: repair of DNA Crosslinks Date: 7 Dec 1995 09:35:55 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi recombinogenic people: About reparation of DNA I also have some questions about the role of rad1-rad10 in sacharomyces and about XPF-ERCC1 in Humans (?) Are this complexes really equal in their role? As far as I know they cut the damaged zone in 3' but I dont know of what kind of lession in DNA? Which enzime cuts in 5'? Which are the homologous in E. coli if any? Thanks in advance Edgar Valencia Morales CIFN - UNAM MEXICO e-mail edgar@cifn.unam.mx From owner-recombination@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!agate!news.Stanford.EDU!nntp-hub2.barrnet.net!newsfeed.internetmci.com!in1.uu.net!pipeline!psinntp!psinntp!news.nstn.ca!newsflash.concordia.ca!news.mcgill.ca!VM1.MCGILL.CA From: chow Newsgroups: bionet.molbio.recombination Subject: RE: Request on Site specific Recombination by Bob Lansman Date: 11 DEC 95 10:56:31 EST Organization: McGill University Lines: 34 Sender: usenet@MUSICA.MCGILL.CA Message-ID: <11DEC95.11817589.0112@VM1.MCGILL.CA> References: <01BAC3EA.5CDD98E0@pool2_4.odyssee.net> NNTP-Posting-Host: vm1.mcgill.ca In article <01BAC3EA.5CDD98E0@pool2_4.odyssee.net> dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) writes: > > >---------- >From: PMDF Mail Server, by way of Bob Lansman [SMTP:postmaster@VAXA.CIS.UWOSH.EDU] >Sent: Wednesday, December 06, 1995 8:36 AM >To: recom@net.bio.net >Subject: (none) > > > > >Date: Tue, 05 Dec 1995 15:28:25 -0600 >From: Bob Lansman > > >In response to the "anything interesting in recombinant DNA" thread, G.D. >mentioned systems in which tissue-specific conditional knockouts could be >produced. I'm extremely interested in this possibility. Can anyone steer >me to a reference or two to get me started. > >A second and completely unrelated question. A couple of weeks ago, just as >the Recom list was being formed, I asked whether anyone knows how DNA cross >links, such as the photoproduct of psoralen, are repaired. No one answered >so I'm asking again. Thanks. Bob Lansman > > > > > > >. >. From owner-recombination@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!agate!news.ucdavis.edu!library.ucla.edu!newsfeed.internetmci.com!in1.uu.net!pipeline!psinntp!psinntp!news.nstn.ca!newsflash.concordia.ca!news.mcgill.ca!VM1.MCGILL.CA From: chow Newsgroups: bionet.molbio.recombination Subject: RE: Request on Site specific Recombination by Bob Lansman Date: 11 DEC 95 11:06:27 EST Organization: McGill University Lines: 42 Sender: usenet@MUSICA.MCGILL.CA Message-ID: <11DEC95.11996324.0112@VM1.MCGILL.CA> References: <01BAC3EA.5CDD98E0@pool2_4.odyssee.net> NNTP-Posting-Host: vm1.mcgill.ca In article <01BAC3EA.5CDD98E0@pool2_4.odyssee.net> dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) writes: > > >---------- >From: PMDF Mail Server, by way of Bob Lansman [SMTP:postmaster@VAXA.CIS.UWOSH.EDU] >Sent: Wednesday, December 06, 1995 8:36 AM >To: recom@net.bio.net >Subject: (none) > > > > >Date: Tue, 05 Dec 1995 15:28:25 -0600 >From: Bob Lansman > > >In response to the "anything interesting in recombinant DNA" thread, G.D. >mentioned systems in which tissue-specific conditional knockouts could be >produced. I'm extremely interested in this possibility. Can anyone steer >me to a reference or two to get me started. > >A second and completely unrelated question. A couple of weeks ago, just as >the Recom list was being formed, I asked whether anyone knows how DNA cross >links, such as the photoproduct of psoralen, are repaired. No one answered >so I'm asking again. Thanks. Bob Lansman > > Last week Szatmari George had provided a mechanism of repair of DNA cross-links such as psoralen. That is it involves the participation of both excision repair i.e. the uvsABC proteins and recombination repair i.e. the recBCD and recA proteins in prokaryotes such as E. coli. For eukaryotes, base on the genetic data and some biochemical experimental results, the same mechanism appears to hold. Any other suggestions on the mechanism? Terry Chow e-mail: mdty@musica.mcgill.ca > > >. >. From owner-recombination@net.bio.net Sun Dec 10 22:00:00 1995 Path: biosci!agate!news.ucdavis.edu!rocky.ucdavis.edu!fznewt From: Jeff 'Newt' Sekelsky Newsgroups: bionet.molbio.recombination Subject: Re: rad1-rad10 Date: Sun, 10 Dec 1995 12:30:30 -0800 Organization: University of California, Davis Lines: 59 Message-ID: NNTP-Posting-Host: rocky.ucdavis.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Sender: fznewt@rocky.ucdavis.edu In-Reply-To: On 7 Dec 1995, Edgar Valencia wrote: > Hi recombinogenic people: > About reparation of DNA I also have some questions about the role of > rad1-rad10 in sacharomyces and about XPF-ERCC1 in Humans (?) > Are this complexes really equal in their role? > As far as I know they cut the damaged zone in 3' but I dont know > of what kind of lession in DNA? > Which enzime cuts in 5'? > Which are the homologous in E. coli if any? > Thanks in advance > > Edgar Valencia Morales > CIFN - UNAM > MEXICO > e-mail edgar@cifn.unam.mx > The Rad1/Rad10 endonuclease is believed to make the 5' incision in NER: Matsunaga T; Mu D; Park CH; Reardon JT; Sancar A. (1995) Human DNA repair excision nuclease. Analysis of the roles of the subunits involved in dual incisions by using anti-XPG and anti-ERCC1 antibodies. Journal of Biological Chemistry 270):20862-9. Bardwell AJ; Bardwell L; Tomkinson AE; Friedberg EC. (1994). Specific cleavage of model recombination and repair intermediates by the yeast Rad1-Rad10 DNA endonuclease. Science 265:2082-5. Homologs of Rad1 are Rad16 (S. pombe), XPF, ERCC-4, and mei-9 (Drosophila). Homologs of Rad10 are Swi10 (S. pombe), ERCC-1. Rad1 and Rad10 are also required for some types of mitotic recombination; Rad16 and Swi10 are also required for mating type switching; mei-9 is required for meiotic recombination; ERCC-1 knockouts are lethal (which may explain why there is no corresponding XP group). Ivanov EL; Haber JE. (1995). RAD1 and RAD10, but not other excision repair genes, are required for double-strand break-induced recombination in Saccharomyces cerevisiae. Mol.Cell.Bio.15:2245-51. etc. McWhir J; Selfridge J; Harrison DJ; Squires S; Melton DW. (1993). Mice with DNA repair gene (ERCC-1) deficiency have elevated levels of p53, liver nuclear abnormalities and die before weaning. Nature Genetics 5: 217-24. XPG makes the 3' incision: O'Donovan A; Davies AA; Moggs JG; West SC; Wood RD. (1994. XPG endonuclease makes the 3' incision in human DNA nucleotide excision repair. Nature 371:432-5. Homologs of XPG are Rad2 (S. cerevisiae), Rad13 (S. pombe) and ERCC-5. - Jeff Sekelsky From owner-recombination@net.bio.net Mon Dec 11 22:00:00 1995 Path: biosci!GPU.SRV.UALBERTA.CA!hastings From: hastings@GPU.SRV.UALBERTA.CA (P Hastings) Newsgroups: bionet.molbio.recombination Subject: Release of engineered organisms Date: 12 Dec 1995 10:31:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 52 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I seem to have dumped the discussion that began last week on the concerns which are arround about the release of transgenic plants. But my memory is that there is much more to be said, so I want to add a couple of points and perhaps get some answers from someone out there. I always find myself in a conflict because I hold that it would be rediculous to ban genetically altered organisms, since this is merely the natural successor to plant breeding. Yet it clearly opens new possibilities, some of which are dangerous. My bottom line position is that it has to be done properly anmd with a broad perspective and sense of responsibility beyond the immediate. Dellaire raised the point that antibiotic resistance remaining in an organism may induce allergy. What then of BST in our milk or B.t. toxin in our potatoes. On the latter point, my local paper said "experts are agreed that it is non-toxic" so you will be pleased to know that I am clearly defined as non-expert. But I could be so easily satisfied- if they took the trouble to use a leaf -specific promoter then I have no problem with it. Did they? And if not, why not? And why would anyone be allowed to release a potato in which such a simple precaution had not been taken? There is no need to leave to antibiotic resistance genes intact. Permits for release of such plants should require their removal. Don't say "you don't know how difficult these things are". I do know but I would also say that if we do not know how to do something like this, then we are not ready to release the organism. Then again, why are these things not marked? In Canada we even colour-code our wheat varieties. But now there are B. t. toxin producing potatoes which are indistinguishable from russet burbank unless you have a DNA lab. How could we recall them if it were to turn out to be a mistake? I am taking too much of your time, so let me quickly put in another point for disscussion before closing- Criteria for whether a gene will spread in the wild and cause harm are pitifully inadequate. The possibilities of wildy wide horezontal transmission would make an interesting dicussion, but even within the realm of conventional genetics, you cannot establish that a gene will not spread to related wild population by planting a testplot and watching the others nearby to see whether they pick up your marker. Take the case of glyphosate resistant rapeseed. Noone may claim that hybridization with wild Brassicas is not possible. It looks as if any Brassica hybrid is possible- just some are going to be extremely rare. How many times did bread wheat arrise in the wild? What about Spartina townsendii? Such wide hybrids are rare and highly improbable accidents, but they have occurred. So the question should be not whether glyphosate resistance can spread, but rather, does it matter if it does? Allright ,I know this isn't about recombination. But I didn't raise the subject and our group may have valid points of view. Phil Hastings From owner-recombination@net.bio.net Tue Dec 12 22:00:00 1995 Path: biosci!agate!news.ucdavis.edu!chicken.engr.ucdavis.edu!njshih From: njshih@chicken.engr.ucdavis.edu (Neng-Jen Shih) Newsgroups: bionet.molbio.recombination Subject: rRNA isolation Date: 13 Dec 1995 06:51:34 GMT Organization: College of Engineering - University of California - Davis Lines: 7 Message-ID: <4alt5m$dvs@mark.ucdavis.edu> NNTP-Posting-Host: chicken.engr.ucdavis.edu X-Newsreader: TIN [version 1.2 PL2] Dose anyone working with RNA know any protocol that work with rRNA isolation from the animal tisue? Please e-mail me if you know. Thanks in advance. Remi njshih@chicken.engr.ucdavis.edu From owner-recombination@net.bio.net Wed Dec 13 22:00:00 1995 Path: biosci!agate!news.ucdavis.edu!library.ucla.edu!news.bc.net!news.uoregon.edu!tank.news.pipex.net!pipex!newsfeed.internetmci.com!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: REpost Release of Engineered Organisms (Phil Hastings) Date: 13 Dec 1995 22:07:12 GMT Organization: McGill University Lines: 59 Message-ID: <4aniqg$hp3@sifon.cc.mcgill.ca> NNTP-Posting-Host: g-12.das.mcgill.ca X-Newsreader: SPRY News 3.03 (SPRY, Inc.) Repost of Phils post.... seems that not all news feeds are equall. Although this post made it to bionet it did not make it to McGill's news feed. I seem to have dumped the discussion that began last week on the concerns which are arround about the release of transgenic plants. But my memory is that there is much more to be said, so I want to add a couple of points and perhaps get some answers from someone out there. I always find myself in a conflict because I hold that it would be rediculous to ban genetically altered organisms, since this is merely the natural successor to plant breeding. Yet it clearly opens new possibilities, some of which are dangerous. My bottom line position is that it has to be done properly anmd with a broad perspective and sense of responsibility beyond the immediate. Dellaire raised the point that antibiotic resistance remaining in an organism may induce allergy. What then of BST in our milk or B.t. toxin in our potatoes. On the latter point, my local paper said "experts are agreed that it is non-toxic" so you will be pleased to know that I am clearly defined as non-expert. But I could be so easily satisfied- if they took the trouble to use a leaf -specific promoter then I have no problem with it. Did they? And if not, why not? And why would anyone be allowed to release a potato in which such a simple precaution had not been taken? There is no need to leave to antibiotic resistance genes intact. Permits for release of such plants should require their removal. Don't say "you don't know how difficult these things are". I do know but I would also say that if we do not know how to do something like this, then we are not ready to release the organism. Then again, why are these things not marked? In Canada we even colour-code our wheat varieties. But now there are B. t. toxin producing potatoes which are indistinguishable from russet burbank unless you have a DNA lab. How could we recall them if it were to turn out to be a mistake? I am taking too much of your time, so let me quickly put in another point for disscussion before closing- Criteria for whether a gene will spread in the wild and cause harm are pitifully inadequate. The possibilities of wildy wide horezontal transmission would make an interesting dicussion, but even within the realm of conventional genetics, you cannot establish that a gene will not spread to related wild population by planting a testplot and watching the others nearby to see whether they pick up your marker. Take the case of glyphosate resistant rapeseed. Noone may claim that hybridization with wild Brassicas is not possible. It looks as if any Brassica hybrid is possible- just some are going to be extremely rare. How many times did bread wheat arrise in the wild? What about Spartina townsendii? Such wide hybrids are rare and highly improbable accidents, but they have occurred. So the question should be not whether glyphosate resistance can spread, but rather, does it matter if it does? Allright ,I know this isn't about recombination. But I didn't raise the subject and our group may have valid points of view. Phil Hastings From owner-recombination@net.bio.net Sun Dec 17 22:00:00 1995 Path: biosci!agate!news.duke.edu!godot.cc.duq.edu!newsfeed.pitt.edu!uunet!in2.uu.net!fdn.fr!jussieu.fr!citi2.fr!news From: villartay@citi2.fr Newsgroups: bionet.molbio.yeast,bionet.molbio.recombination,bionet.molbio.methds-reagnts,bionet.immunology Subject: Conference on DNA recombination Date: Mon, 18 Dec 95 13:32:57 PDT Organization: CITI2 - Universite Rene Descartes, Paris Lines: 26 Message-ID: <4b3o40$ruc@bisance.citi2.fr> NNTP-Posting-Host: cayman.necker.fr Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII To: laudat@tolbiac.inserm.fr X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage Xref: biosci bionet.molbio.yeast:4391 bionet.molbio.recombination:81 bionet.molbio.methds-reagnts:37736 bionet.immunology:6669 ------------------------------------------------------------------------------- - ####### INSERM CONFERENCES PHILIPPE LAUDAT ###### - - - - - - "V(D)J Recombination and other models of DNA Repair and Mutagenesis" - - - - October 13-17, 1996 - - Aix-Les-Bains, France - - - - - - For more informations please contact: - - Ms Colette BREZIN - - Ms Yolande RICHARD - - INSERM Tel+fax : 33-1-44-23-63-89 - - e-Mail : Laudat@tolbiac.inserm.fr - ------------------------------------------------------------------------------- ------------------------------------------------------------------------------- Could you please print this announcement and post it on the seminar board of your institution. Thank you in advance. Jean-Pierre de Villartay (villartay@citi2.fr) From owner-recombination@net.bio.net Sun Dec 17 22:00:00 1995 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.recombination Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 18 Dec 1995 02:00:30 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199512181000.CAA20676@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-recombination@net.bio.net Mon Dec 18 22:00:00 1995 Path: biosci!PASTEUR.HJF.ORG!msalminen From: msalminen@PASTEUR.HJF.ORG (Mika Salminen) Newsgroups: bionet.molbio.recombination Subject: *** WWW Search of HIV Seq. & PCR Primers Date: 19 Dec 1995 13:37:59 -0800 Organization: Henry M. Jackson Foundation Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: <30D730AC.6330@hiv.hjf.org> Reply-To: msalminen@pasteur.hjf.org NNTP-Posting-Host: net.bio.net We are pleased to announce that we have significantly updated the Webpage of The Global Molecular Epidemiology Group at the Henry M. Jackson Foundation for the Advancement of Military Medicine and the Division of Retrovirology, Walter Reed Army Institute of Research, Rockville, MD. 20850 New features include: * Search and download primer sequences for amplification and sequencing of HIV-1 gag and env genes. Search by clade or for general panel. Includes options for restricting search and incorporates measures on primer performance (based on actual records in our lab) * New manuscript describing "Bootscanning" - a method for the detection of recombination among viral sequences * HIV-1 Phylogenetic trees * Alignments of HIV-1 gag and env genes, both in Pretty and Computer readable format The webpage adress is: http://hivgenome.hjf.org/ Please have a look! Mika Salminen, Ph.D. Research Fellow Henry M. Jackson Foundation and the MMCARR Retrovirology, Global Molecular Epidemiology 1600 East Gude Drive, 20850 Rockville, MD Phone: 301-217-9410, ext. 1045 Fax: 301-762-7460 From owner-recombination@net.bio.net Mon Dec 18 22:00:00 1995 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!newsfeed.internetmci.com!chi-news.cic.net!nntp.coast.net!oleane!in2p3.fr!univ-lyon1.fr!jussieu.fr!citi2.fr!news From: villartay@citi2.fr Newsgroups: bionet.molbio.yeast,bionet.molbio.recombination,bionet.molbio.methds-reagnts,bionet.immunology Subject: Conf. on DNA recombination (erratum) Date: Tue, 19 Dec 95 08:55:57 PDT Organization: CITI2 - Universite Rene Descartes, Paris Lines: 27 Message-ID: <4b5rst$pe7@bisance.citi2.fr> NNTP-Posting-Host: cayman.necker.fr Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII X-Newsreader: NEWTNews & Chameleon -- TCP/IP for MS Windows from NetManage Xref: biosci bionet.molbio.yeast:4394 bionet.molbio.recombination:82 bionet.molbio.methds-reagnts:37774 bionet.immunology:6678 correction to the first announcement of this conference!!!!!!!!! ------------------------------------------------------------------------------- - ####### INSERM CONFERENCES PHILIPPE LAUDAT ###### - - - - - - "V(D)J Recombination and other models of DNA Repair and Mutagenesis" - - - - October 13-17, 1996 - - Aix-Les-Bains, France - - - - - - For more information please contact: - - Ms Colette BREZIN - - Ms Yolande EYOUM - - INSERM Tel+fax : 33-1-44-23-60-89 - - e-Mail : Laudat@tolbiac.inserm.fr - ------------------------------------------------------------------------------- ------------------------------------------------------------------------------- Could you please print this announcement and post it on the seminar board of your institution. Thank you in advance. Jean-Pierre de Villartay (villartay@citi2.fr)