From owner-recombination@net.bio.net Fri Feb 02 22:00:00 1996 Path: biosci!bloom-beacon.mit.edu!newsxfer2.itd.umich.edu!newsfeed.internetmci.com!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: Workshop on Transgenic Plants Date: 3 Feb 1996 21:25:58 GMT Organization: McGill University Computing Centre Lines: 201 Message-ID: <4f0jt6$qv3@sifon.cc.mcgill.ca> NNTP-Posting-Host: e-05.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) ---------------------------------------------------------------------------- Workshop on Transgenic Plants: Biology and Applications __________________________________________________________ April 20-22, 1996 Kellogg Conference Center Tuskegee University An Invitation to Attend a Workshop on Transgenic Plants: Please pass this information to others through any means possible such as "redirecting" to your favorite newsgroups, colleagues and students and posting on notice boards. Sorry for the multiple postings! ----------------------------------------------------------------------------= - Tuskegee University, Alabama, USA will be hosting a workshop (supported by NSF and USDA) on transgenic plants during April 20-22, 1996. More than 25 eminent scientists will be presenting lectures on the current status of research on transgenic plants including fundamental biology, techniques, crop improvement, commercial applications, ethical issues and socio-economic considerations. There also will be two panel discussions: (1) funding opportunities; and (2) strategies for enhancing minority participation in plant molecular biology research. Additional attractions include Demonstration of the Plant Genome Database, 'How Internet Can Enhance Your Research Productivity', Commercial Exhibits from Biotechnology Companies, Representatives from Various Ph. D. Programs around the Country with Information on Graduate Assistantships, and a Tour of the Plant Molecular Genetics Laboratory at Tuskegee University. A limited travel funding is available for underrepresented minority undergraduate and graduate students from U. S. who can present posters from their research at the workshop. Please contact Dr. C. S. Prakash immediately for information on this funding. Workshop on Transgenic Plants: Biology and Applications __________________________________________________________ April 20-22, 1996 Kellogg Conference Center Tuskegee University Schedule of the Workshop Saturday April 20, 1996 7 PM: Reception/Cocktail (Supported by Private Companies) Registration Sunday, April 21, 1996 8 AM: Plenary Lecture Ganesh Kishore, Monsanto Co. "Crop Improvement Via Biotechnology" Eugene Nester, University of Washington "Agrobacterium Biology" Roger Beachy, Scripps Research Institute "Genetic Engineering of Virus Resistance in Plants" Trevor Thorpe, University of Alberta "Somatic Embryogenesis" Maureen Hanson, Cornell University "Transgenic Plants and Developmental Biology" =46red Perlak, Monsanto "Engineering Insect Resistance in Plants" G. Kakefuda, American Cyanamid "Designing Herbicide Tolerance in Plants" Virginia Walbot, Stanford University "Transposable Elements" Hans Bohnnert, University of Arizona "Transgenic Plants and Abiotic Stress" Brian Larkins, University of Arizona "Enhancing Protein Quality in Crops" Susan Wessler, University of Georgia "Transposable Elements Can Be Our Friends" Chris Somerville, Carnegie Institute of Washington, Stanford U "Biodegradable Plastics from Transgenic Plants" Banquet Lecture Charles Arntzen, Boyce Thompson Plant Research Institute "Transgenic Plants Producing Edible Vaccines" Monday, April 22, 1996 K. V. Raman, ISAAA, Cornell University "International Applications of Transgenic Plants" Rob Fincher, Pioneer Hi-Bred International "Commercial Applications of Transgenic Plants " =46red Buttell, University of Wisconsin, Madison " Socioeconomic Aspects of Transgenic Plants" Paul Thompson, Texas A& M University "Ethical Considerations in Transgenic Plant Research" Terry Medley, USDA/APHIS "Regulatory Issues for Field and Public Release of Transgenic Plants= " Panel Discussion 1: "Enhancing Minority Participation in Transgenic Plant Research and Education" Frank Greene, USDA/ARS Walter Hill, Tuskegee University Terry Medley, USDA/APHIS Jerome Roberts, Alabama A & M University Panel Discussion 2: "Funding Opportunities for Research on Transgenic Plants= " Program Managers from USDA, NSF, DOE and NASA The workshop will conclude at 5 PM on Monday, April 22. (note: all the above speakers are confirmed. Some lecture titles are tentative and subject to change) Additional Speakers to be confirmed Pamela Ronald- University of California, Davis Tim Conner, Monsanto Co. Robert Jones, University of Minnesota Maria Ragland, Research Genetics, Inc. Elloy Rodriguez, Cornell University Location of the Meeting: The venue is The Kellogg Executive Conference Center, Tuskegee University, Old Montgomery Road, Tuskegee, AL 36088, USA. It is a new and very modern conference facility with 180 luxury rooms. Contact the Center directly to reserve your room soon. Toll Free#: 1-800-949-6161 (334-727-3000; Fax 334-727-5119). Room rates are $70 Single and $80 Double. Credit Cards Accepted. Reduced rates available for U. S. Government Employees. Information on less expensive but farther hotels (for those with own transportation) is available from Dr. Prakash. Registration: Subsidized Conference Registration is $100 and includes admittance to all sessions, cocktail, meals (continental breakfast and luncheon on Sunday and Monday; banquet on Sunday, coffee and snacks during breaks) and the abstract book. You can pay on site but you must have confirmed registration (see form below). Getting There: Special discounted air travel rates are available. Please contact Meeting Management Services at 1-800-821-8751. Atlanta Airport is located about 120 miles (about 2 hours drive) from Tuskegee and has excellent flight connections. Montgomery (Ala.) is about 40 minutes drive. Shuttle service from the airport is available; Call the conference center or call Dixie Excursions at 334-887-6294. Car rental is an attractive option as it provides greater flexibility and costs are comparable to the shuttle. Weather: We will order some good sunny weather for you. You may expect 60-75=B0F (15-22=B0C) during April in Tuskegee. Those interested in attending the workshop, please complete the following form with your name, address, email, fax and phone numbers and forward to: Dr. C. S. Prakash "Workshop on Transgenic Plants" School of Agriculture and Home Economics Milbank Hall, Tuskegee University Tuskegee, AL 36088-1641. USA Email: Prakash@Acd.Tusk.Edu Phone (334) 727 8023 =46ax (334) 727 8552 Remember pre-registration is necessary! You may pay $100 registration on site (credit card, cash or check). Clip and forward to Prakash@Acd.Tusk.Edu ----------------------------------------------------------------------------= ---- Name: Title: Address: Email: Phone: =46ax Number: Any dietary or other restrictions............................... I am interested in presenting a poster : ------------Yes ---------------No (For minority student travel grant consideration, include an abstract and a statement of research interests) ------------------------------------------------------------------------ C. S. Prakash Phone (334) 727 8023 Tuskegee University Fax (334) 727 8552 School of Agriculture Email: Prakash@Acd.Tusk.Edu Tuskegee, AL 36088. USA ------------------------------------------------------------------------- From owner-recombination@net.bio.net Fri Feb 02 22:00:00 1996 Path: biosci!news.Stanford.EDU!nntp-hub2.barrnet.net!newsfeed.internetmci.com!news.msfc.nasa.gov!elroy.jpl.nasa.gov!swrinde!howland.reston.ans.net!psinntp!psinntp!psinntp!news.nstn.ca!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: JOB Listing for IRCM (Montreal) 3 Positions Date: 3 Feb 1996 04:15:29 GMT Organization: McGill University Lines: 78 Message-ID: <4eunh1$7bl@sifon.cc.mcgill.ca> NNTP-Posting-Host: b-10.das.mcgill.ca X-Newsreader: SPRY News 3.03 (SPRY, Inc.) Job Listing #1: GENE THERAPY AND MOLECUALR GENETICS OF HEMATOPOIETIC STEM CELLS Guy Sauvageau, M.D., Ph.D., FRCP(C) Institut de Recherche Clinique de Montreal (IRCM) Montreal, Quebec, Canada Project description: (description en francais) Our laboratory explores the hypothesis that transcription factors which control proliferation and differentiation of embryonic tissues may also regulate these processes in normal and possibly abnormal (e.g. leukemic, etc.) hematopoiesis. The following objectives are being persued in our laboratory: 1.to study the function of selected transcription factors whose expression is restricted to primitive hematopoietic stem cells, 2.to use these genes in order to manipulate the behaviour of hematopoietic stem cells and, 3.to identify and develop novel strategies to optimize gene transfer to these cells. These goals are being addressed by using different experimental strategies such as experimental bone marrow traplantation procedures (murine model), retroviral gene transfer, generation of cDNA library starting from a single cell, in vitro differentioation of embryonic stem (ES) cells and generation of transgenic and knock mice. Positions: *Post Doctoral Position required Ph.D. in cellular or molecular biology *Ph.D. Position (MRC studentship available) required molecular biology/biochemistry background and grade point average greater 3.65 *Summer Student Position possibility of persuing graduate studies after summer session Notes: *English or French as a minimum language reguirement To Apply: Please contact Dr. Guy Sauvageau Vox: (514) 987 5797 Fax (514) 987 5688 E-mail: sauvagg@ircm.umontreal.ca Curriculum Vitae and most recent transcripts will be requested. GENETIQUE MOLEDULAIRE DES CELLULES SOUCHES HEMOPOIETIQUES ET THERAPIE GENIQUE Guy Sauvageau, M.D., Ph.D., FRCP(C) Les mécanismes molé ulaires responsables de la prlifération et de la différentiation des cellules souches hémopoiétiques normales et leucémiques demeurent encore mal caractérisés. Les objectifs de notre laboratoire sont: 1.d'étudier la fonction de certain faceurs de transcription dont l'expression est spécifique aux cellules les plus primitives de la moelle osseuse, 2.d'utilieser ces gènes nouvellement caractérisés pour générer des cellules souches hemopoiétiques ayant une capcité accrue de repopulation, 3.de développer de nouvelles stratégies pour optimiser le transfert de gènes à ces cellules. Les principaux modèles expérimentaux utilisées pour ces études incluent la tranplantation de moelle osseuse (modèle murin), le transfert de gène(s) à l'aide de rétrovirus recombinants, la génération de libraires d'ADN complémentaires à partir d'une seule cellule purifiée, la différentiation in vitro de cellules embryonnaire de souris (ES), et la génération de souris transgéniques et "knock out". From owner-recombination@net.bio.net Sat Feb 03 22:00:00 1996 Path: biosci!news.Stanford.EDU!nntp-hub2.barrnet.net!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!swidir.switch.ch!scsing.switch.ch!news.belwue.de!news.uni-stuttgart.de!news From: Kulic Igor Newsgroups: bionet.molbio.recombination Subject: Methylase-Problem Date: 4 Feb 1996 15:39:49 GMT Organization: University of Stuttgart Lines: 29 Message-ID: <4f2k05$2e4a@info4.rus.uni-stuttgart.de> NNTP-Posting-Host: rusxppp181.rus.uni-stuttgart.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Please help ! Does anyone of you know which nucleases perform methylisation of nucleotids and where it is comercially availuable ? - How specific can the mathylisation be in practice : i.e is there a similar variety of Methylases like there is for Class II restriction enzymes ? -A more specific question is wheather there is a known methylase that performs the following site-speciffic methylisation depending strongly of the recognition sequence (like GGAATTC or AAGCTT): m m GAATTC ----> GAATTC or AAGCTT ---> AAGCTT -Are there analogous principles for de-mathylisation known (i.e. Sequence-speciffic) These questions are in some way essential for a theoretic work on possible DNA-computing mechanisms I am working on . I would be allso very happy and thakfull for usefull refernces about principles of methylasation ,if any are known ! Thank you in advance Kindly yours Igor From owner-recombination@net.bio.net Mon Feb 05 22:00:00 1996 Path: biosci!biochem.kth.se!mostafa From: mostafa@biochem.kth.se (Mostafa Ronaghi) Newsgroups: bionet.molbio.recombination Subject: Polymerases Date: 6 Feb 1996 01:48:16 -0800 Organization: Biochemistry, Royal Institute of Technology Lines: 22 Sender: daemon@net.bio.net Distribution: world Message-ID: <31172346.41C67EA6@biochem.kth.se> NNTP-Posting-Host: net.bio.net Dear all: I would like to know which DNA polymerase has the highest fidelity and processivity? I would like also ask anyone about the reverse activity of RNA polymerases (T7 RNA polymerses has this kind of activity as I have read). To clarify my question I can probabely presice the problem. "if RNA polymerase get just one, two, or three nucleotides (but not 4, which result in run-off) it will synthese the RNA until it will reach the nucleotide which hasn't been added, then probabely it will stop elongation because of its high fidelity! My question is "HOW RNA POLYMERASE ACTS AT THIS MOMENT" Will it go back on DNA template (Reverse activity)?? in this case Does RNA-strand separates and jumps off from DNA strands, and RNA polymerase get a new chance for making another RNA with the presnt nucleotides? Thanks in advance. Best regards, mostafa@biochem.kth.se From owner-recombination@net.bio.net Mon Feb 05 22:00:00 1996 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!usc!sdd.hp.com!swrinde!newsfeed.internetmci.com!vixen.cso.uiuc.edu!uchinews!news.luc.edu!orion.it.luc.edu!ecline From: ecline@orion.it.luc.edu (Elizabeth Cline) Newsgroups: bionet.molbio.recombination Subject: we Date: 5 Feb 1996 20:00:39 GMT Organization: Loyola University Chicago Lines: 1 Message-ID: <4f5nl7$o4e@artemis.it.luc.edu> NNTP-Posting-Host: 147.126.1.9 NNTP-Posting-User: ecline hi From owner-recombination@net.bio.net Wed Feb 07 22:00:00 1996 Path: biosci!ATLAS.ODYSSEE.NET!dellaire From: dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: Job Listing (Lady Davis Institute, McGill University) Date: 7 Feb 1996 19:00:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 58 Sender: daemon@net.bio.net Distribution: world Message-ID: <3119640E.2EDB@odyssee.net> NNTP-Posting-Host: net.bio.net Job Listing #2: Characterization of The RNA Binding Ability of SAM68 Dr.Stéphan Richard, Ph.D Lady Davis Institute for Medical Research Montreal, Quebec, Canada Project description: The focus of our laboratory is on signal transduction mediated by tyrosine kinases. We have previously discovered Sam68 which is a fyn, src family tyrosine kinase which binds SH3 domains. Further we have demonstrated that many other proteins with SH3 and SH2 domains bind Sam68 including ras-GTPase activating protein (GAP), phospholipase C-1 and Grb2 (Mol. Cell. Biol. 1995, 15:186). Sam68 defines a family of proteins that bind signal transduction molecules and RNA. Interestingly, phosphorylation of this protein abolishes its ability to bind RNA(J. Biol. Chem. 1995, 270:2010). Specifically the aims of the project are: 1.to delineate the RNA binding domains of Sam68 and to understand the mechanism by which phosphorylation abolishes RNA binding. 2.to identify physiologcal targets for the RNA binding daomain of Sam68. The student will gain knowledge in signal transduction, as well as techniques in molecular biology, cell culture, phosphorylation and immunology. Positions: Ph.D. Postion -to qualify, applicants must have a B.Sc. in molecular biology, biochemistry or immunology in addition to obtaining a GPA of greater than 3.4. To Apply: Please send your curriculum vitae and a copy of your transcript to: Dr. Stéphan Richard Lady Davis Institute 3755 Cote St. Catherine Road Montreal, Quebec Canada H3T 1E2 mcrd@musica.mcgill.ca (for more jobs in the Montreal Area try our web site....) http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html From owner-recombination@net.bio.net Thu Feb 08 22:00:00 1996 Path: biosci!ERE.UMontreal.CA!szat From: szat@ERE.UMontreal.CA (Szatmari George) Newsgroups: bionet.molbio.recombination Subject: lox/cre plasmids Date: 9 Feb 1996 11:12:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <199602091909.OAA24581@alize.ERE.UMontreal.CA> NNTP-Posting-Host: net.bio.net Dear readers, This is in response to a previous query about the lox/cre recombination system. This site-specific recombination system has recently been shown to be active in a variety of organisms. Some of these recent references are: (use in plants) Qin et al 1994, Proc Natl Acad Sci 91:1706-1710 (in mice) Araki et al 1995, Proc Natl Acad Sci 92: 160-164 (in human cell lines) Anton and Graham 1995, J Virol 69: 4600-4606 Of course, for any original work on this system, there are tons of references originating from the DuPont group headed by Nat Sternberg. George Szatmari Universite de Montreal From owner-recombination@net.bio.net Tue Feb 13 22:00:00 1996 Path: biosci!ATLAS.ODYSSEE.NET!dellaire From: dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: Job posting for Montreal (McGIll University Ph.D. 5 years funding guaranteed) Date: 14 Feb 1996 15:23:47 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 84 Sender: daemon@net.bio.net Distribution: world Message-ID: <9602142320.AA16148@odyssee.net> NNTP-Posting-Host: net.bio.net For other job listings try... http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html Today's listing from Montreal. Job Listing #3: Ph.D. Project involving Mouse Transgenics Dr. Hans H. Zingg, M.D., Ph.D Royal Victoria Hospital Montreal, Quebec, Canada Project description: Dr. Hans has one MRC studentship voucher for which he can guaranteed a 5-year MRC studentship to a qualified student of his choice. Areas of research 1. Production of an oxytocin receptor knock-out mouse 2. Molecular mechanisms controlling oxytocin receptor gene expression 3. Oxytocin signalling mechanisms Recent publications: 1.Uterine oxytocin gene expression: I. Induction during pseudopregnancy and during the estrous cycle. Endocrinology 134: 2556-2561, 1994. 2.Uterine oxytocin gene expression: II. Induction by exogenous steroid administration. Endocrinology 134: 2562-2566, 1994. 3.Structure, characterization and expression of the rat oxytocin receptor gene. Proc. Natl. Acad. Sci. USA 92: 200-204, 1995. 4.Establishment, characterization and co-culture of non-transformed cell lines derived from rat endometrial epithelium and stroma. In Vitro Cellular and Develpmental Biology 31: 140-148, 1995. 5.Oxytocin and oxytocin receptor gene expression in the uterus. In: "Recent Progress in Hormone Research", 50th Laurential Hormone Conference, Academic Press, San Diego, Vol. 50, pp. 255-273, 1995. Positions: Ph.D. Postion Qualifications: B.Sc. with solid background in molecular and cellular biology. B.Sc. in Biochemistry is an advantage, but high achievment in any allied field will also be considered favorable. g.p.a.: 3.5 of higher. To Apply: For further information please contact: Hans H. Zingg, M.D., Ph.D. E-mail: zingg@rvhri.lan.mcgill.ca Tel: (514) 843 1553 From owner-recombination@net.bio.net Tue Feb 13 22:00:00 1996 Path: biosci!rutgers!oitnews.harvard.edu!das-news2.harvard.edu!news4.ner.bbnplanet.net!news.ner.bbnplanet.net!howland.reston.ans.net!newsfeed.internetmci.com!info.ucla.edu!nnrp.info.ucla.edu!usenet From: brian@ewald.mbi.ucla.edu (Brian Vicente) Newsgroups: bionet.molbio.recombination Subject: oligomers Date: Wed, 14 Feb 1996 01:48:44 GMT Organization: University of California, Los Angeles Lines: 2 Message-ID: <4frf17$11t6@saba.info.ucla.edu> NNTP-Posting-Host: dsepc2.mbi.ucla.edu X-Newsreader: Forte Free Agent 1.0.82 Does anyone knows how to replicate dna?? From owner-recombination@net.bio.net Thu Feb 15 22:00:00 1996 Path: biosci!bch.univie.ac.at!gw From: gw@bch.univie.ac.at (Georg Weitzer) Newsgroups: bionet.molbio.recombination Subject: FIAU source wanted Date: 16 Feb 1996 08:07:41 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199602161607.IAA07196@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone know from which company I can get, or buy, FIAU for research purpose? From owner-recombination@net.bio.net Thu Feb 15 22:00:00 1996 Path: biosci!ATLAS.ODYSSEE.NET!dellaire From: dellaire@ATLAS.ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: replicating DNA.... Date: 15 Feb 1996 17:48:53 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: <9602160145.AA23324@odyssee.net> NNTP-Posting-Host: net.bio.net Dear Brian, In what sense? What do you want to replicate? You can use PCR.... or ligate the dna into a bacterial vector and amplify in bacteria or use Klenow and random primers (say if you wanted to make a probe). Tell us more? Graham From owner-recombination@net.bio.net Sat Feb 17 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.recombination Subject: BIOSCI miniFAQ, ver. 14-DEC-95 Date: 18 Feb 1996 02:00:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 199 Sender: daemon@net.bio.net Distribution: world Message-ID: <199602181000.CAA06727@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 14-DEC-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. Contents: -------- 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index in addition to the master index for the entire set. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-recombination@net.bio.net Mon Feb 19 22:00:00 1996 Path: biosci!CS.Arizona.EDU!news.Arizona.EDU!hawker.medmicro.arizona.edu!ralph From: ralph@ccit.arizona.edu (R M Bernstein) Newsgroups: bionet.molbio.recombination Subject: recombination in the immune system Date: Tue, 20 Feb 96 14:49:10 GMT Organization: University of Arizona Dept of Micro/Immuno Lines: 17 Message-ID: NNTP-Posting-Host: hawker.medmicro.arizona.edu X-Newsreader: VersaTerm Link v1.1.5 Hello all, I was wondering if anyone has seen the recent work out of M. Gehlert's lab. They are now saying that the recombinase activating gene 1 actively binds the nonamer, while r2 performs the coding/signal joint nick. Did anyone go to the recent meeting where they described this in detail? And is this still in accord with phage INT mediated recombination? Regards, Ralph R.M. Bernstein Dept of Micro/Immuno University of Arizona Ph: 602 626 2585 Fx: 602 626 2100 url: http://lamprey.medmicro.arizona.edu http://radon.gas.uug.arizona.edu/~bernster From owner-recombination@net.bio.net Thu Feb 22 22:00:00 1996 Path: biosci!bdg10.niddk.nih.gov!KAYUWEW From: KAYUWEW@bdg10.niddk.nih.gov ("WAGNER, KAY-UWE") Newsgroups: bionet.molbio.recombination Subject: Sorry, here is the URL Date: 23 Feb 1996 06:44:28 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <312DD239@SMTP2.mm.hub.nih.gov> NNTP-Posting-Host: net.bio.net Sorry, i forgot to mention the URL of the mammary gland database: http://alice.dcrt.nih.gov/~mammary/ Kay From owner-recombination@net.bio.net Thu Feb 22 22:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: URL for Mammary Gene Expression Data Base Date: 22 Feb 1996 19:13:03 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BB0172.5507E1A0@pool13_4.odyssee.net> NNTP-Posting-Host: net.bio.net Sorry for the repeat but "none" was not descriptive a title... Again the URL for the resource described by Kay Wagner is http://condor.mbcr.bcm.tmc.edu/BEP/ERMB/home.html Previously Kay mentioned: A new web site was established: Biology of the Mammary Gland Database Purpose: This Web site serves as a forum to integrate various aspects of Mammary Gland Biology, to promote collaborations and the exchange of ideas, knowledge and resources. It was established in October of 1995 by the Section of Developmental Biology at the National Institutes of Diabetes, Digestive and Kidney Diseases (National Institutes of Health, Bethesda, Maryland, USA). What's Here : Mammary Gland Database Bulletin Board Knock-out Mouse of the Month Transgenic Mouse of the Month Development (includes transgenic and null mice with a mammary phenotype) mini reviews Genetics Gene Regulation Biotechnology Tools (features technologies and reagents) Research Groups Literature Art and History Relation to the RECOM group: You can find some general information about the Cre recombinase and the Tet system under "reviews" and "tools" of this database. From owner-recombination@net.bio.net Thu Feb 22 22:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: RE: (none) Date: 22 Feb 1996 19:07:52 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 47 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BB0171.9A31AE60@pool13_4.odyssee.net> NNTP-Posting-Host: net.bio.net Thanks Kay, I found the URL for everyone it is at: http://condor.mbcr.bcm.tmc.edu/BEP/ERMB/home.html Briefly it contains again as Kay Wagner described: A new web site was established: Biology of the Mammary Gland Database Purpose: This Web site serves as a forum to integrate various aspects of Mammary Gland Biology, to promote collaborations and the exchange of ideas, knowledge and resources. It was established in October of 1995 by the Section of Developmental Biology at the National Institutes of Diabetes, Digestive and Kidney Diseases (National Institutes of Health, Bethesda, Maryland, USA). What's Here : Mammary Gland Database Bulletin Board Knock-out Mouse of the Month Transgenic Mouse of the Month Development (includes transgenic and null mice with a mammary phenotype) mini reviews Genetics Gene Regulation Biotechnology Tools (features technologies and reagents) Research Groups Literature Art and History Relation to the RECOM group: You can find some general information about the Cre recombinase and the Tet system under "reviews" and "tools" of this database. From owner-recombination@net.bio.net Thu Feb 22 22:00:00 1996 Path: biosci!bdg10.niddk.nih.gov!KAYUWEW From: KAYUWEW@bdg10.niddk.nih.gov ("WAGNER, KAY-UWE") Newsgroups: bionet.molbio.recombination Subject: (none) Date: 22 Feb 1996 18:09:23 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: <312D2141@SMTP2.mm.hub.nih.gov> NNTP-Posting-Host: net.bio.net A new web site was established: Biology of the Mammary Gland Database Purpose: This Web site serves as a forum to integrate various aspects of Mammary Gland Biology, to promote collaborations and the exchange of ideas, knowledge and resources. It was established in October of 1995 by the Section of Developmental Biology at the National Institutes of Diabetes, Digestive and Kidney Diseases (National Institutes of Health, Bethesda, Maryland, USA). What's Here : Mammary Gland Database Bulletin Board Knock-out Mouse of the Month Transgenic Mouse of the Month Development (includes transgenic and null mice with a mammary phenotype) mini reviews Genetics Gene Regulation Biotechnology Tools (features technologies and reagents) Research Groups Literature Art and History Relation to the RECOM group: You can find some general information about the Cre recombinase and the Tet system under "reviews" and "tools" of this database. From owner-recombination@net.bio.net Sun Feb 25 22:00:00 1996 Newsgroups: bionet.molbio.recombination Path: biosci!lhc.nlm.nih.gov!nih-csl!Moshe.Sadofsky From: sadofsky@helix.nih.gov (Moshe Sadofsky) Subject: Re: recombination in the immune system Message-ID: <1996Feb26.183559.27417@alw.nih.gov> X-Posted-From: InterNews 1.0@nih-csl.dcrt.nih.gov. Lines: 11 Sender: -Not-Authenticated-[4156] Nntp-Posting-Host: vdj3.niddk.nih.gov Organization: NIH/NIDDK/LMB References: Date: Mon, 26 Feb 1996 18:35:59 GMT Xdisclaimer: No attempt was made to authenticate the sender's name. Ralph Bernstein wanted update on VDJ recombination. I am one of the post docs in the Gellert lab. The current lab model, as presented at the DNA recombination meeting in Taos 2/10/96, indicates a direct association between RAG-1 protein and the nonamer component of the signal sequence. I have DNA crosslinking data to support this. We do not yet have any data that assigns a biochemical role in nicking to either of the two proteins. That is an obvious priority. Therefore, while nicking at the heptamer is a plausible activity of RAG-2, it is purely speculative at this moment. -Moshe Sadofsky (sadofsky@helix.nih.gov) From owner-recombination@net.bio.net Wed Feb 28 22:00:00 1996 Path: biosci!news.Stanford.EDU!tera.mcom.com!news.uoregon.edu!news.dacom.co.kr!newsfeed.internetmci.com!howland.reston.ans.net!nntp.coast.net!fu-berlin.de!news.dfn.de!news.ruhr-uni-bochum.de!news.rhrz.uni-bonn.de!rhrz-ts1-p15.rhrz.uni-bonn.de!unb600 From: unb600@ibm.rhrz.uni-bonn.de (Achim Plum) Newsgroups: bionet.molbio.recombination Subject: Keratinocyte culture and transfection Date: Wed, 28 Feb 1996 20:17:38 Organization: University of Bonn, Germany Lines: 8 Message-ID: NNTP-Posting-Host: rhrz-ts1-p15.rhrz.uni-bonn.de X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] I am planning to culture mouse keratinocytes (such as Hel30 and Hel37) and transfect them with various constructs. Has anyone got additional information on these cell lines (apart from ATCC) i.e. transfection parameters? Also, I am interested in other mouse skin derived cell lines. Thanks! a.plum@uni-bonn.de From owner-recombination@net.bio.net Wed Feb 28 22:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: FW: Keratinocyte culture and transfection Date: 29 Feb 1996 15:45:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 54 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BB06D5.5D27A2E0@pool24_9.odyssee.net> NNTP-Posting-Host: net.bio.net ---------- From: Graham Dellaire[SMTP:dellaire@odyssee.net] Sent: Thursday, February 29, 1996 6:24 PM To: 'Achim Plum' Subject: RE: Keratinocyte culture and transfection ---------- From: Achim Plum[SMTP:unb600@ibm.rhrz.uni-bonn.de] Sent: Wednesday, February 28, 1996 3:17 PM To: recom@net.bio.net Subject: Keratinocyte culture and transfection I am planning to culture mouse keratinocytes (such as Hel30 and Hel37) = and=20 transfect them with various constructs. Has anyone got additional = information on these cell lines (apart from ATCC) i.e. transfection parameters? = Also, I am=20 interested in other mouse skin derived cell lines. Thanks! a.plum@uni-bonn.de I have never transfected mouse keratinocytes but I have transfected many = a mouse fibroblast cells line (LTA, LM tk- etc). There is quite a lot = of data in our lab to do with various methods (CaPO4, DEAE-dextran and = electroporation). It might help if you could tell us what is the application?... ie is it = transient expression or stable clones that you want etc. =20 Graham Dellaire dellaire@odyssee.net P.S. I haven't found ATCC's descriptions that helpful either! By the = way has anyone ever characterized or seen a characterization for LTA (L = cells) mouse fibroblasts? I am doing FISH right now and there are alot = of metacentrics...etc and there must be 50+ chromosomes!!! From owner-recombination@net.bio.net Thu Feb 29 22:00:00 1996 Path: biosci!bch.univie.ac.at!gw From: gw@bch.univie.ac.at (Georg Weitzer) Newsgroups: bionet.molbio.recombination Subject: FIAU-Where can I buy it? Date: 1 Mar 1996 00:22:03 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <199603010821.AAA22690@net.bio.net> NNTP-Posting-Host: net.bio.net Which company sells FIAU or Gancyclovir? (Oclassen no longer supplies us with FIAU) Please, contact me via e-mail if you have the slightest idea wher to get this stuff. GEORG WEITZER