From owner-recombination@net.bio.net Mon Apr 01 23:00:00 1996 Path: biosci!ns1.faseb.org!lamarck.sura.net!newsfeed.internetmci.com!uwm.edu!lll-winken.llnl.gov!nntp.coast.net!swidir.switch.ch!scsing.switch.ch!rzunews.unizh.ch!NewsWatcher!user From: hubscher@vetbio.unizh.ch (Ulrich Hubscher) Newsgroups: bionet.molbio.methds-reagnts,bionet.general,bionet.genome.chromosomes,bionet.molbio.proteins,bionet.molbio.yeast,bionet.virology,bionet.molbio.recombination Subject: ANNOUNCE: EMBO Workshop on Molecular Biology of DNA Replication Date: Tue, 02 Apr 1996 16:39:07 +0100 Organization: Universitat Zurich-Irchel Lines: 46 Message-ID: NNTP-Posting-Host: 130.60.120.18 Xref: biosci bionet.molbio.methds-reagnts:42570 bionet.general:20910 bionet.genome.chromosomes:1104 bionet.molbio.proteins:7493 bionet.molbio.yeast:5039 bionet.virology:6861 bionet.molbio.recombination:153 EMBO WORKSHOP: MOLECULAR BIOLOGY OF DNA REPLICATION September 8 - 13, 1996 Weggis (Luzern), Switzerland Organizers: U. Hubscher, Zurich P. Plevani, Milano S. Spadari, Pavia The main topics which will be covered are: Regulatory mechanisms controlling entry into S-phase; replisome assembly and its functional organization within the nucleus; protein-protein interactions among replication proteins; structural organization of the replisome; connection between DNA replication and other DNA transactions (e.g. repair, transcription). Keynote address: Bruce Stillman Cold Spring Harbor Laboratory New York, USA The registration fee of CHF 850. includes all meals, lodging and social events. A certain amount of the budget will be reserved to partially refund the registration fee to young applicants. Appllcations contalnlng one page abstract shoud be sent as soon as possible but not later than May 31st, 1996 to: EMBO WORKSHOP 96: Universitat Zurich-lrchel, Institut fur Veterlnarbiochemie Winterthurerstrasse 190 CH-8057 Zurich, Switzerland _____________________________________________________________________ Ulrich Hubscher Institut fur Veterinarbiochemie Tel: (41-1)-257-54-72 Universitat Zurich-Irchel Fax: (41-1)-257 59 04 Winterthurerstrasse 190 CH-8057 Zurich Switzerland _____________________________________________________________________ From owner-recombination@net.bio.net Wed Apr 03 23:00:00 1996 Path: biosci!rutgers!csn!news-1.csn.net!imci3!imci4!newsfeed.internetmci.com!info.ucla.edu!library.ucla.edu!news.ucdavis.edu!boris.ucdavis.edu!not-for-mail From: ez014377@boris.ucdavis.edu (Daniel Anderson) Newsgroups: bionet.molbio.recombination Subject: Re: Info On Homologous recombination Date: 3 Apr 1996 19:50:25 GMT Organization: University of California, Davis Lines: 12 Distribution: world Message-ID: <4jukq1$h03@mark.ucdavis.edu> References: <3159C961.1060@odyssee.net> NNTP-Posting-Host: boris.ucdavis.edu X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Graham Dellaire (dellaire@ODYSSEE.NET) wrote: : For a good overview of recombination of all kinds try to find this book: Dont forget S Kowaczykowski et al review on the Biochemistry of H R. Biochemistry of Homologous Recombination in Escherichia coli Microbiological Reviews, Sept. 1994, p.401-465 Vol. 58, No. 3 -Dan From owner-recombination@net.bio.net Sun Apr 07 23:00:00 1996 Path: biosci!PO-BOX.MCGILL.CA!popa0206 From: popa0206@PO-BOX.MCGILL.CA Newsgroups: bionet.molbio.recombination Subject: Replication EMBO workshop!!! (repost;.... server troubles) Date: 8 Apr 1996 13:53:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604082051.QAA02634@sirocco.CC.McGill.CA> NNTP-Posting-Host: net.bio.net THis is a repost for Ulrich EMBO WORKSHOP: MOLECULAR BIOLOGY OF DNA REPLICATION September 8 - 13, 1996 Weggis (Luzern), Switzerland Organizers: U. Hubscher, Zurich P. Plevani, Milano S. Spadari, Pavia The main topics which will be covered are: Regulatory mechanisms controlling entry into S-phase; replisome assembly and its functional organization within the nucleus; protein-protein interactions among replication proteins; structural organization of the replisome; connection between DNA replication and other DNA transactions (e.g. repair, transcription). Keynote address: Bruce Stillman Cold Spring Harbor Laboratory New York, USA The registration fee of CHF 850. includes all meals, lodging and social events. A certain amount of the budget will be reserved to partially refund the registration fee to young applicants. Appllcations contalnlng one page abstract shoud be sent as soon as possible but not later than May 31st, 1996 to: EMBO WORKSHOP 96: Universitat Zurich-lrchel, Institut fur Veterlnarbiochemie Winterthurerstrasse 190 CH-8057 Zurich, Switzerland _____________________________________________________________________ Ulrich Hubscher Institut fur Veterinarbiochemie Tel: (41-1)-257-54-72 Universitat Zurich-Irchel Fax: (41-1)-257 59 04 Winterthurerstrasse 190 CH-8057 Zurich Switzerland _____________________________________________________________________ From owner-recombination@net.bio.net Mon Apr 08 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: JOB LISTINGS NEW FOR APRIL!!! (McGill University!) Date: 9 Apr 1996 16:40:43 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 189 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BB264B.44D78BC0@pool10_13.odyssee.net> NNTP-Posting-Host: net.bio.net Here are a few of the job listings this month at=20 ExpMed Job Listings=20 http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html **************************************************************** Job Listing #3=20 Ph.D. Project Involving Role of Nitric Oxide (NO) in Cellular Iron = Metabolism Prem Ponka, M.D., Ph.D=20 Lady Davis Institute For Medical Research=20 Sir Mortimer B. Davis-Jewish General Hospital Montreal, Quebec, Canada=20 Project description:=20 Dr. Ponka has one MRC studentship voucher for which he can guaranteed a = 5-year MRC studentship to a qualified student of his choice.=20 Areas of research=20 1.Cellular iron metabolism=20 role of iron in cell proliferation=20 role of iron in free radical formation=20 role of nitric oxide (NO) in cellular iron metabolism=20 2.Development of new iron chelators of significance to:=20 iron overload=20 inhibition of free radical reactions=20 neoplastic cell replication 3.Erythroid differentiation=20 4.Heme sythesis regulation Recent publications:=20 Richard, D.R., Neumannova, V., and Ponka, P.: Nitrogen monoxide = decrease iron uptake from transferrin but does not mobilise iron from prelabelled neoplastic cells.=20 Biochim. Biophys. Acta 1266:250-260 (1995) Richard, D.R., Neumannova, V., Nagy, E., and Ponka, P.: The effect = of redox-related species of nitrogen monoxide on transferrin and iron uptake and cellular proliferation = of erythroleukemia (K562) cells.=20 Blood 86: 3211-3219 (1995)=20 Loyevksy, M., Tsafack, A., Ponka, P., and Cabantchik, Z.I.: Mode of = action of iron (III) chelators as antimalarials. IV. Potentiation of Desferal action by benzoyl- and = isonicotinoyl-hydrazone derivative. J. Lab. Clin. Med. (in press) Positions: Ph.D. Postion=20 Qualifications: B.Sc. with solid background in molecular and = cellular biology. B.Sc. in Biochemistry is an advantage, but high achievment in any allied field will also = be considered favorable. g.p.a.: 3.5 of higher.=20 To Apply: For further information please contact:=20 Prem Ponka, M.D., Ph.D.=20 Lady Davis Institute For Medical Research=20 Sir Mortimer B. Davis-Jewish General Hospital 3755 Cote St. Catherine Road Montreal, Quebec, Canada H3T 1E2=20 Tel: (514) 340 8260=20 Fax: (514) 340 7502=20 ******************************************************************** Post-Doctoral Projects involving Cell and Molecular Biology of = Neurodegenerative Disease Dr. Andr=E9a LeBlanc, Ph.D=20 Lady Davis Institute for Medical Research=20 Montreal, Quebec, Canada=20 Project description:=20 2 POST-DOCTORAL POSITIONS IN CELL AND MOLECULAR BIOLOGY OF = NEURODEGENERATIVE DISEASES=20 Projects Amyloid =DF peptide toxicity of human neurons.=20 Regulation of amyloid precursor protein metabolism in human = neurons, and astrocytes. Molecular mechanisms of prion protein.=20 Positions: Two Post Doctoral Positions.=20 Requirements for the Candidate: Must have a Ph.D. in either biochemistry, molecular biology, = cell biology, or neuroscience with technical experience in at least two of the four categories.=20 To Apply: Send Curriculum Vitae and 2-3 letters of recommendation to:=20 Dr. Andr=E9a LeBlanc, Lady Davis Institute for Medical Research,=20 Sir Mortimer B. Davis Jewish General Hospital, 3755 Ch. Cote Ste-Catherine, Montreal, Quebec, Canada.=20 H3T 1E2. *Include your telephone, Fax and e-mail numbers.=20 *************************************************************** Job Listing #5:=20 Molecular Biology of Parathyroid Gland Activity Dr. Geoffrey N. Hendy=20 Calcium Research Lab Royal Victoria Hospital=20 Montreal, Quebec, Canada=20 Project description: A studentship is available in the laboratory of Dr. Geoffry Hendy as a = part his operating grant entitled=20 "Investigation of the Molecular Biology of Parathryoid Gland Activity" Positions: 1 Ph.D. position TO START SUMMER 1996=20 Prospective graduate students interested in recieving an MRC Studentship = should send a curriculum vitae together with a transcript of their academic records. This studenship is for a student = preferably to undertake a Ph.D. under Dr. Hendy's supervision although candidates for M.Sc. will also be considered. The = studentship is available to start graduate studies preferably for the summer of 1996.=20 To Apply: Please send your curriculum vitae and a copy of your transcript to:=20 Dr. Geoffrey N. Hendy=20 Calcium Research Lab., Rm. H4.67 Royal Victoria Hospital Montreal, Quebec Canada H3A 1A1 From owner-recombination@net.bio.net Mon Apr 08 23:00:00 1996 Path: biosci!daresbury!imbb1.imbb.forth.gr!nefeli.imbb.forth.gr!ANASTASO From: anastaso@nefeli.imbb.forth.gr Newsgroups: bionet.plants,bionet.molbio.recombination Subject: tobacco mutant seeds Date: Tue, 09 Apr 1996 18:36:11 GMT Organization: Imbb-forth,CREtE, creece Lines: 25 Message-ID: <009A0999.9929E85D@nefeli.imbb.forth.gr> Reply-To: anastaso@nefeli.imbb.forth.gr NNTP-Posting-Host: nefeli.imbb.forth.gr Xref: biosci bionet.plants:10866 bionet.molbio.recombination:156 Dear Netters, we are to mutagenise TOBACCO seeds and then select for the mutants In order to map the position of the mutation we would need to tobacco plants that carry known markers; ones that are known to reside at particular chromosomes would be very usefull to our mapping effort. Therefore may we ask anyone who has seeds from such plants to send us some to the address mentioned below. Thank you for your help. Elias Anastassopoulos, Foundation of Research and Technology-Hellas Institute of Molecular Biology and Biotechnology P.O.Box 1527, Heraklion, 711 10, Crete, HELLAS. Fax # (081) 230469 email:anastaso@nefeli.imbb.froth.gr From owner-recombination@net.bio.net Mon Apr 08 23:00:00 1996 Path: biosci!daresbury!imbb1.imbb.forth.gr!nefeli.imbb.forth.gr!ANASTASO From: anastaso@nefeli.imbb.forth.gr Newsgroups: bionet.molbio.recombination Subject: tobacco mutant seeds (email correction) Date: Tue, 09 Apr 1996 22:26:00 GMT Organization: Imbb-forth,CREtE, creece Lines: 25 Message-ID: <009A09B9.B4296334@nefeli.imbb.forth.gr> Reply-To: anastaso@nefeli.imbb.forth.gr NNTP-Posting-Host: nefeli.imbb.forth.gr Dear Netters, we are to mutagenise TOBACCO seeds and then select for the mutants In order to map the position of the mutation we would need tobacco plants that carry known markers; ones that are known to reside at particular chromosomes would be very usefull to our mapping effort. Therefore may we ask anyone who has seeds from such plants to send us some to the address mentioned below. Thank you for your help. Elias Anastassopoulos, Foundation of Research and Technology-Hellas Institute of Molecular Biology and Biotechnology P.O.Box 1527, Heraklion, 711 10, Crete, HELLAS. Fax # (081) 230469 email:anastaso@nefeli.imbb.forth.gr From owner-recombination@net.bio.net Sat Apr 13 23:00:00 1996 Path: biosci!rutgers!gatech!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: Phaedrus <75017.1050@CompuServe.COM> Newsgroups: bionet.general,bionet.molbio.recombination Subject: Networking transgene injectionists Date: 14 Apr 1996 03:00:32 GMT Organization: CompuServe, Inc. (1-800-689-0736) Lines: 24 Message-ID: <4kppoh$dap$1@mhadg.production.compuserve.com> Xref: biosci bionet.general:21113 bionet.molbio.recombination:159 Hi My name is Gary Brown, working at present out of Wash. U at St Louis in a lab heavily involved in the generation of transgenic animals by both pronuclear microinjection and ES cell injection into blastocysts. So, what's this all about? Well, I'm contemplating writing a WWW Homepage resource directed at all those individuals out there with a technical interest in microinjection, with possibly a technique of the month feature, jobs offered, jobs wanted etc. I need to get an idea of what people might be interested in seeing on such a page. That's where maybe you, the reader of my wee scribble, might wish to contribute. Also, the thought did enter my head that should people actually respond here that there might exist the possibility of connecting each other via an E-newsletter of sorts. Your thoughts please. I believe that persons who do microinjection might be well served by their own organizational body. Yet again, thoughts are appreciated. Nearly done. One more thing. I'm not affiliated with any sleazebag headhunter types IN ANY WAY, and of course would not use any responses to generate a list for anyone other than me. Thanx, Gary (75017.1050@compuserve.com) From owner-recombination@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!swsbe6.switch.ch!scsing.switch.ch!news.belwue.de!news.uni-stuttgart.de!news From: "Igor M. Kulic" Newsgroups: bionet.molbio.recombination Subject: Invertase activity and supercoiling Date: Wed, 17 Apr 1996 13:48:48 +0200 Organization: UNI-Stuttgart Lines: 20 Message-ID: <3174DAA0.41C6@cip.mathematik.uni-stuttgart.de> NNTP-Posting-Host: cip14.mathematik.uni-stuttgart.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (X11; I; AIX 2) Does anybody know any refereces or is possibly able to help me with following question : Which Inv sites on a supercoiled plasmid are more likely to be recombined by invertase . I guess that sites that are closer to each other (and have the right orietation ) are more likely to react than those that are far apart . For example we have the following (lineary written ) plasmid with 3 Inv sites (where "X" denotes the Inv-site and "Y" his inverted ) ..X...Y.....Y.............. (start and end-positions should be identified ) Is "X" generaly more likely to react with the first "Y"-site than with the second ? How strong does this probablity dependent on supercoilng of the plasmid ? I would be very thankful for any hint or relevant reference if any are known . From owner-recombination@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!daresbury!not-for-mail From: Johnjoe McFadden Newsgroups: bionet.molbio.recombination Subject: transposable elements and speciation Date: 17 Apr 1996 10:00:47 +0100 Lines: 26 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <4l2bvv$6dk@mserv1.dl.ac.uk> X-Sender: bss1jm@central.surrey.ac.uk Original-To: recom@dl.ac.uk I am interested in the possibility of transposable elements being involved in speciation events. Does anybody know of examples of species or genera-specific insertion sequences or transposable elements from prokaryotes or eukaryotes? A few examples that I am aware of are IS200 in Salmonella species, IS6110 in Mycobacterium tuberclulosis complex and IS900 in Mycobacterium paratuberculosis. Any others? Johnjoe McFadden Johnjoe McFadden, PhD Molecular Microbiology Group School of Biological Sceinces University of Surrey, Guildford, Surrey GU2 5XH, UK. tel: 44-(0)1483 300800 extn.2671 fax: 44-(0)1483 300374 e-mail: j.mcfadden@surrey.ac.uk From owner-recombination@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!MAIL.UMU.SE!hong From: hong@MAIL.UMU.SE (Hong Qian) Newsgroups: bionet.molbio.recombination Subject: Looking for protein Date: 17 Apr 1996 02:47:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604170944.AA07557@mail.umu.se> NNTP-Posting-Host: net.bio.net Hello everyone out there: I am a Ph D. student working for protein structure determination by NMR method. I had finished calculating one protein structure which contains 90 amino acids. Now I am looking for a new protein. If someone has an over-expressed protein which is suitable for NMR study, I would be very happy working for it. The protein used for NMR structure calculation must fufill the following conditions: 1. Solvable at mili-molar range in water. 2. Stable at room temperature. 3. Molecular weight below 30 kD 4. If it contains more than 70 amino acids, it should be isotopic labelled by nitrogen 15 or both nitrogen-15 and carbon-13. Generally, we need around 10 mg protein for NMR study. Of course, it depends on molecular weight of protein. If anyone interest in this, please contact with me by email. Hong Qian NMR Research Group Department of Organic Chemistry Umea University S- 901 87, Umea Sweden email: hong@indro.chem.umu.se tel: 46-90-166936 fax: 46-90-138885 From owner-recombination@net.bio.net Tue Apr 16 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: RE: transposable elements and speciation Date: 17 Apr 1996 09:00:11 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BB2C53.C1D6AD00@pool3_1.odyssee.net> NNTP-Posting-Host: net.bio.net ---------- From: Johnjoe McFadden[SMTP:j.mcfadden@surrey.ac.uk] Sent: Wednesday, April 17, 1996 5:00 AM To: recom@net.bio.net Subject: transposable elements and speciation >I am interested in the possibility of transposable elements being = involved >in speciation events. >Does anybody know of examples of species or genera-specific insertion >sequences or transposable elements from prokaryotes or eukaryotes? Johnjoe McFadden, PhD Molecular Microbiology Group School of Biological Sceinces University of Surrey, Guildford, Surrey GU2 5XH, UK. -------------------------------------------------------------------------= ---------------------------------------- Johnjoe, Retroposons are very similar to transposons but move via an RNA = intermediate (i.e. are reverse trascribed first as suggested by poly aaa = tail) Line-1 retroposons are only in mammals ((Lmd in mice and Line-1 in = primates so far) and I think Alu's have a similar occurance (B-1 in mice = and Alu in man). =20 Just another little note: Another interesting thing about Lines and Alu elements is that they are = segregated into late (heterochromatin) and early (euchromatin) = replicating Dna bands in mitotic chromosomes, respectively. =20 To me this indicates perhaps a role in genome evolution and or = structure. Hope this helps, G. Dellaire dellaire@odyssee.net Dept. of Medicine Div. of Exp. Medicine McGill University From owner-recombination@net.bio.net Wed Apr 17 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.recombination Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 18 Apr 1996 02:00:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199604180900.CAA27292@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-recombination@net.bio.net Thu Apr 18 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: RE: transposable elements and speciation Date: 19 Apr 1996 06:40:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BB2DD2.E8AAA460@pool3_6.odyssee.net> NNTP-Posting-Host: net.bio.net ---------- From: Ashok Aiyar[SMTP:aiyar@ebv.oncology.wisc.edu] Sent: Wednesday, April 17, 1996 5:29 PM To: dellaire@ODYSSEE.NET Subject: Re: transposable elements and speciation [This message has also been posted.] On 17 Apr 1996 09:00:11 -0700, Graham Dellaire = wrote: >Retroposons are very similar to transposons but move via an RNA=20 >intermediate (i.e. are reverse trascribed first as suggested by poly = aaa >tail) > >Line-1 retroposons are only in mammals ((Lmd in mice and Line-1 in >primates so far) and I think Alu's have a similar occurance (B-1 in = mice >and Alu in man). Has anyone actually known an Alu to transpose in human cells? I=20 know of one experiment (published in Cell, around Dec. 1987) that purported to show Alu transposition, but this result was later shown to be likely caused by BLUR8 contamination. Curious, Ashok -- Ashok Aiyar Department of Oncology aiyar@ebv.oncology.wisc.edu Univ. of Wisconsin-Madison tel: (608) 262-6697 I am not sure about alu's as they do not encode their own RT or = integrase (recombinase) so it is harder to think what events might lead = up to their activation and consequent move to a new site...=20 Line-1 's on the other hand encode 2 ORF's and one of them is a putative RT gene. It is believed that one or two proto lines in the mammalian = genome are able to transpose (retrotranspose that is) during = embryological development. I think their was some work done in sperm of = mice that indicated that this movement occurred quite readily. I will = try to get the reference soon. G. Dellaire From owner-recombination@net.bio.net Sun Apr 21 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: POSTDOC AND PHD positions in MONTREAL (NEW) Date: 22 Apr 1996 16:48:45 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 178 Sender: daemon@net.bio.net Distribution: world Message-ID: <01BB3083.33323CC0@cisco2_8.odyssee.net> NNTP-Posting-Host: net.bio.net Job Listing Identification of Putative Target sites of Repressor/Activators in Yeast Dr. Bernard Turcotte Assistant Professor, Dept. of Medicine McGill University Montreal, Quebec, Canada Project description: Among the organisms that are used as models for the genome project, yeast (S. cerevisae) is the first one with its complete sequence determined. Given the fact that yeast has a small genome size (14 Mbps), few intronic sequences and that many processes are highly conserved from yeasst to man, it constitutes a powerful model. As a result, many studies oare aimed at identifying the role of the various open reading frames (ORF's) revealed by the yeast sequencing project. However, less attention has been paid to the analysis of promoter regions. Since these regions are rather short and can be easily located, yeast offers a good model to study these sequences. The aim of the project is to identify putative target sites fro activators (or repressors) of gene transcription by searching a database with DNA sequences that are recognized by zinc finger proteins. Predictions made from the search will then be verified experimentally. These studies should allow the identification of vaarious gene networks. Positions: 1 Ph.D. position and 1 Post-doctoral position Financial support is currently available. Funding being provided for the project by the Canadian Genome Analysis and Technology Program Prospective post-doctoral candidates or graduate students interested in the above project should send a curriculum vitae together with a transcript of their academic records. To Apply: For further information please contact: Dr. Bernard Turcotte Assistant Professor, Dept. of Medicine, McGill University Molecular Endocrinology Laboratory Room H7.82 Royal Victoria Hospital 687 Pine Ave. West Montreal H3A 1A1 Tel. 514-842-1231 Ext. 5046 FAX: 514-982-0893 E-mail: turcotte@lan1.molonc.mcgill.ca --------------------------------------------------------------------------- Job Listing Cell Signalling Mechanisms with respect to Insulin and Growth Factor Action Dr. Barry Posner Polypeptide Laboratory McGill University Montreal, Quebec, Canada Project description: A studentship award is available for the study of cell signalling mechanisms focused particularly on insulin and growth factor action. Positions: 1 Ph.D. position Prospective graduate students should send a curriculum vitae together with a transcript of their academic records. DEADLINE MAY 15TH 1996 To Apply: Please send your curriculum vitae and a copy of your transcript to: Dr. Barry Posner Polypeptide Laboratory McGill University 3640 University St. Rm W 315 Montreal, Quebec Canada H3A 2B2 --------------------------------------------------------------------------------------------- Job Listing Ph.D. Project Involving Role of Nitric Oxide (NO) in Cellular Iron Metabolism Prem Ponka, M.D., Ph.D Lady Davis Institute For Medical Research Sir Mortimer B. Davis-Jewish General Hospital Montreal, Quebec, Canada Project description: Dr. Ponka has one MRC studentship voucher for which he can guaranteed a 5-year MRC studentship to a qualified student of his choice. Areas of research 1.Cellular iron metabolism role of iron in cell proliferation role of iron in free radical formation role of nitric oxide (NO) in cellular iron metabolism 2.Development of new iron chelators of significance to: iron overload inhibition of free radical reactions neoplastic cell replication 3.Erythroid differentiation 4.Heme sythesis regulation Recent publications: Richard, D.R., Neumannova, V., and Ponka, P.: Nitrogen monoxide decrease iron uptake from transferrin but does not mobilise iron from prelabelled neoplastic cells. Biochim. Biophys. Acta 1266:250-260 (1995) Richard, D.R., Neumannova, V., Nagy, E., and Ponka, P.: The effect of redox-related species of nitrogen monoxide on transferrin and iron uptake and cellular proliferation of erythroleukemia (K562) cells. Blood 86: 3211-3219 (1995) Loyevksy, M., Tsafack, A., Ponka, P., and Cabantchik, Z.I.: Mode of action of iron (III) chelators as antimalarials. IV. Potentiation of Desferal action by benzoyl- and isonicotinoyl-hydrazone derivative. J. Lab. Clin. Med. (in press) Positions: Ph.D. Postion Qualifications: B.Sc. with solid background in molecular and cellular biology. B.Sc. in Biochemistry is an advantage, but high achievment in any allied field will also be considered favorable. g.p.a.: 3.5 of higher. To Apply: For further information please contact: Prem Ponka, M.D., Ph.D. Lady Davis Institute For Medical Research Sir Mortimer B. Davis-Jewish General Hospital 3755 Cote St. Catherine Road Montreal, Quebec, Canada H3T 1E2 Tel: (514) 340 8260 Fax: (514) 340 7502 ---------------------------------------------------------------------------------------------- From owner-recombination@net.bio.net Sun Apr 21 23:00:00 1996 Newsgroups: bionet.molbio.recombination Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!swsbe6.switch.ch!scsing.switch.ch!news.belwue.de!fu-berlin.de!news.dfn.de!newsjunkie.ans.net!newsfeeds.ans.net!howland.reston.ans.net!newsfeed.internetmci.com!in1.uu.net!sangam!iitb!powai!niharika.phy.iitb.ernet.in!krishnan From: krishnan@PROBLEM_WITH_INEWS_DOMAIN_FILE (s krishnan) Subject: Workshop on Biotechnology Sender: news@powai.cc.iitb.ernet.in (news system user) Message-ID: Date: Mon, 22 Apr 1996 10:09:34 GMT Organization: Computer Centre, IIT Bombay - 400 076, India X-Newsreader: TIN [version 1.2 PL2] Lines: 36 International Symposium on 'Cyanobacterial Biotechnology', September 18-21, 1996 Venue: National Facility for Marine Cyanobacteria Bharathidasan University Tiruchirapalli 620 024 India Review papers covering all aspects Cyanobacterial Biotechnology are invited from 30 senior scientists from different countries. Response so far from 150 scientists all over the world. Interested persons can participate either observers or send abstract for poster presentation immediately. Registration US $ 100 for scientists and US $ 50 for bonafide students. Registration after May 31, 1996 would involve an additional US$ 50 for both categories. Registration fee includes accommodation, food and conference materials for the symposium period. For further details contact Dr. G. Subramanian, Director, National Facility Marine Cyanobacteria, Bharathidasan University, Tiruchirapalli 620 024, India Telephone +91 431 660352, Fax +91 431 660245, E.mail. nfmc@bdu.rect.ernet.in In case, if you have problems with the above email address, send mail to krishnan@phy.iitb.ernet.in From owner-recombination@net.bio.net Tue Apr 30 23:00:00 1996 Path: biosci!SPARC2.CC.NCKU.EDU.TW!s5883105 From: s5883105@SPARC2.CC.NCKU.EDU.TW (Ming-Chuan Hong) Newsgroups: bionet.molbio.recombination Subject: test Date: 1 May 1996 08:38:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605011538.XAA13374@mail.ncku.edu.tw> NNTP-Posting-Host: net.bio.net Test!