From owner-recombination@net.bio.net Fri May 03 23:00:00 1996 Path: biosci!news.Stanford.EDU!nntp-hub2.barrnet.net!newsfeed.internetmci.com!sgigate.sgi.com!news.cs.indiana.edu!news.nstn.ca!newsflash.concordia.ca!news.mcgill.ca!news From: dasuser@PO-Box.McGill.CA Newsgroups: bionet.molbio.recombination Subject: Job In Montreal (post doc) Date: 4 May 1996 21:56:52 GMT Organization: McGill University Computing Centre Lines: 26 Message-ID: <4mgjr4$ljm@sifon.cc.mcgill.ca> NNTP-Posting-Host: d-10.das.mcgill.ca X-Newsreader: SPRY News 3.03 (SPRY, Inc.) **Please DO NOT forward your application via e-mail.*** ======== A postdoctoral position is available in the laboratory of Dr. Guy A. Rouleau at the Montreal General Hospital. The postdoctoral fellow will work on the cell biology of the Neurofibromatosis type 2 protein. Applicants should have experience in cell culture and transfection, cell and molecular biology techniques, and protein analysis. The postdoctoral fellow should be creative, independent and strongly motivated. The postdocotral position is available as soon as possible. Please send your curriculum vitae and statement of research interest to Guy A. Rouleau, M.D., Ph.D. Center for Research in Neuroscience, Montreal General Hospital, Montreal, Quebec, H3G 1A4. **Please DO NOT forward your application via e-mail.*** ======== From owner-recombination@net.bio.net Sun May 05 23:00:00 1996 Path: biosci!ARGOTECH.COM!pvaneikeren From: pvaneikeren@ARGOTECH.COM (Paul van Eikeren) Newsgroups: bionet.molbio.recombination Subject: 1996 GRC on Biocatalysis Date: 5 May 1996 17:13:51 -0700 Organization: Argonaut Technologies Inc. Lines: 174 Sender: daemon@net.bio.net Distribution: world Message-ID: <318D4385.224F@argotech.com> NNTP-Posting-Host: net.bio.net =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Gordon Research Conference B I O C A T A L Y S I S Kimbal Union Academy * Meriden, NH, USA * 7-12 July 1996 =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D http://w3.argotech.com/argotech/biocatalysis I am posting this message to inform you of this years=92 Gordon Research = Conference on Biocatalysis. The Conference will be held 7-12 July 1996 = at Kimbal Union Academy in Meriden, New Hampshire, USA. The Conference = brings together an interdisciplinary group of biologists, chemists, and = engineers for a full week of intense discussion and examination of the = frontiers of Biocatalysis. We welcome your application for = participation in year=92s conference. Also, we would be grateful if you = would forward a copy of this message to people that you think might be = interested in attending. The subject of the Biocatalysis Conference is synthetically useful = reactions and processes catalyzed by enzymes or whole cells. We will = address three main themes: =B7 new uses of existing enzymes (e.g., lipases, oxidases, and aldolases); =B7 discovery of new enzymes (e.g., epoxide hydrolases, P-450 = hydroxylases, oxynitrilases, thermophilic organisms); and =B7 structure and protein engineering of enzymes (e.g., new structures of = proteases, transketolase and oxynitrilase, and combinatorial methods = versus site-directed mutagenesis). = We have assembled a notable group of speakers, discussion leaders, and = poster presenters. Attached is a copy of the preliminary program. For = additional information on the Conference including the most recent = update of the program and the poster sessions we suggest that you = connect to our World-Wide Web site at http://w3.argotech.com/argotech/biocatalysis If you do not have access to the World-Wide Web or need additional = information, please contact me by e-mail at the address shown below and = can send you additional information and applications forms. We look forward to receiving your application to the conference. Sincerely, Paul van Eikeren =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D Paul van Eikeren, Co-Chair Vice President, Chemistry Argonaut Technologies Inc. 887 Industrial Road, Suite G San Carlos, CA 94070 USA Voice: +1 415 598 1350 ext. 217 Fax: +1 415 598 1359 pvaneikeren@argotech.com 74260.1024@compuserve.com =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D= =3D=3D=3D P R O G R A M I N F O R M A T I O N (Updated on 25 March 1996) =3D=3D Opening Session: * Stanley Roberts (Liverpool, UK) Enzymic Baeyer Villiger Reaction * J. John Holbrook (U. Bristol, UK) Getting the Products Off Enzymes = Used in Bulk Chemoenzymic Synthesis =3D=3D Structure and Engineering of Hydrolases for Organic Synthesis * Sabine L. Flitsch (U. Edinburgh, UK) Design and Synthesis of = Enzyme-Cleavable Linkers for Solid Phase Synthesis * Guy G. Dodson (York U., UK) Nucleophilic Attack at the Carbonyl in = Hydrolases: The Varying Stereochemistry at the Nucleophile * Franz Effenberger (U. Stuttgart, Germany) New Results on Preparation = and Application of Chiral Cyanohydrins * Robert Menard (NRC Biotechnology Research Institute, Canada) Protein = Engineering of Cysteine Proteases: From a Better Understanding of Their = Function to Redesigning the Catalytic Activity =3D=3D Molecular Evolution of Subtilisin * Frances Arnold (California Institute of Technology, USA) Directed = Evolution of p-Nitrobenzyl Esterase * Marcus Ballinger (Genentech, USA) Subtilisin BPN Variants Designed for = Cleavage of Multibasic Substrates Multibasic Substrates = * Volker Schellenberger (Genencor, USA) Directed evolution of a = Bacillus protease =3D=3D New Application of Hydrolases * Herbert Waldmann (Karlsruhe, Germany) Bioorganic Synthesis and = Biological Signal Transduction * Milton Zmijewski (Lily, Indianapolis, USA) Enzymatic Removal of = Protecting Groups in the Synthesis of Pharmaceuticals * Kazuo Achiwa (Shizuoka U.) Lipase-Catalyzes Asymmetric Synthesis of = Optically-Active Medicines: New Strategies and their Application =3D=3D Discovery versus Engineering of New Enzymes * John Arnett (Recombinant Biocatalysis, USA) Enzymes from Thermophilic = Microorganisms * Gunter Schneider (Karolinska Institute, Stockholm, Sweden) Toward = Tailoring Enzymes for Asymmetric Synthesis: Protein Engineering of = Transketolase =3D=3D Aldolases and Glycosyl Transfer * Eric Toone (Duke University) Pyruvate Aldolases * Vladimir Kren (Czech Academy of Sciences) Enzymatic Glycosylation of = Pharmacologically Active Compounds: Multienzymatic Approaches and New = Enzymes =3D=3D New Hydrolases * Roland Furstoss (U. Aix-Marseille, France) Enantioselective = Biotransformations with Epoxide Hydrolases * Kenji Soda (Kyoto, Japan) 2-Haloacid Dehalogenases: Their Functions, = Structures, and Applications =3D=3D Oxidations * Aleksey Zaks (Schering-Plough, Union, NJ, USA) Chloroperoxidase * Bernhard Hauer (BASF AG, Ludwigshafen, Germany) Selection of = Biocatalysts for the Preparation of Chiral/Chemical Building Blocks * Roger Sheldon (Delft U. Netherlands) Enantioselective Aminolysis and = Selective Oxidations Mediated by Chloroperoxidase =3D=3D Large Scale Industrial Applications * Kevin DiGregorio (Union Carbide, USA) Polyester Hydrolysis * Robert Dicosimo (Dupont, Wilmington, DE, USA) Scale-Up of a = Biocatalytic Route to N-Phosphonomethylglycine (Glyphosate) Using Whole = Cell Transformants From owner-recombination@net.bio.net Tue May 07 23:00:00 1996 Path: biosci!SPLAVA.CC.PLATTSBURGH.EDU!DECK1548 From: DECK1548@SPLAVA.CC.PLATTSBURGH.EDU (CHRIS DECKER) Newsgroups: bionet.molbio.recombination Subject: Recombination Vectors Date: 8 May 1996 08:31:37 -0700 Organization: SUNY at Plattsburgh, New York, USA Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <01I4GMGZMGQU8ZNYFR@splava.cc.plattsburgh.edu> NNTP-Posting-Host: net.bio.net I am looking for information on the recombination of DNA using viruses as vectors. What I am looking for is current experiments using virues to transmit the DNA that is desired to be recombined with the host cell. Any information would be apreciated. Thanks Chris Decker DECK1548@splava.cc.plattsburgh.edu "Ask Questions" ~TOU~ From owner-recombination@net.bio.net Tue May 07 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET ("Graham Dellaire") Newsgroups: bionet.molbio.recombination Subject: RE: Recombination Vectors Date: 8 May 1996 15:26:49 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 75 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605082224.SAA12517@poseidon.odyssee.net> NNTP-Posting-Host: net.bio.net ---------- > From: DECK1548@splava.cc.plattsburgh.edu (CHRIS DECKER) > To: recom@net.bio.net > Subject: Recombination Vectors > Date: Wednesday, May 08, 1996 11:31 AM > > I am looking for information on the recombination of DNA using viruses as > vectors. What I am looking for is current experiments using virues to > transmit the DNA that is desired to be recombined with the host cell. Any > information would be apreciated. Thanks > > Chris Decker > DECK1548@splava.cc.plattsburgh.edu > "Ask Questions" ~TOU~ > All though many viruses are being used now for gene transfer the grandaddy of them all is the retrovirus... usually mmtlv derivatives. Retro viral vectors provide a relative large host cell range, with the exception that the cell has to be actively dividing, and give high efficiencies. I am not sure what you want exactly... your experiment involves what kind of cells?... Is this a term paper you are researching? A little more info would be useful for myself and any others to really help you. Any way some other viruses that can be used are Adenovirus, which can infect epithelial cells such as those that line the bronchi in your lungs, as well as muscle cells I believe. Adeno associated virus which has the peculiar ability to integrate into Chms 19 more often than not and does not cause the autoimmunity with which adenovirus infected cells are associated. Then their is Herpes virus which can infect neurons in the brain and CNS. Last note, recently researchers have been combining characteristics of of different viruses into so-called ectopic viruses. They may contain the protein coat of one virus and the genome of another. A recent paper in last weeks Science described such a virus that was based on a retro virus but could actually infect non dividing cells (neurons and glial cells). Here are few references: Gene targeting in mouse embryonic stem cells with an adenoviral vector Somatic Cell & Molecular Genetics. 21(4):221-31, 1995 Jul. Gene transfer into hematopoietic progenitor and stem cells: progress and x problems. [Review) Stem Cells. 12(6):563-76, 1994 Nov. Adenovirus-mediated gene transfer into striated muscles. [Review] x Journal of Molecular Medicine. 73(4):165-80, 1995 Apr Adenovirus and adeno-associated virus mediated gene transfer. [Review] xx British Medical Bulletin. 51(1):31-44, 1995 Jan. Gene therapy: adenovirus vectors. [Review] x Current Opinion in Genetics & Development. 3(3):499-503, 1993 Jun. HSV as a gene transfer vector for the nervous system. [Review] x Molecular Biotechnology. 4(1):87-99, 1995 Aug. Gene transfer to brain using herpes simplex virus vectors. [Review] x Annals of Neurology. 35 Suppl:S28-34, 1994. Adeno-associated virus vectors for gene therapy. [Review] xx Gene Therapy. 2(6):357-62, 1995 Aug. From owner-recombination@net.bio.net Wed May 08 23:00:00 1996 Path: biosci!agate!howland.reston.ans.net!vixen.cso.uiuc.edu!chi-news.cic.net!news.luc.edu!orion.it.luc.edu!csriniv From: csriniv@orion.it.luc.edu (Chandra Srinivasan) Newsgroups: bionet.molbio.recombination Subject: : Date: 9 May 1996 03:39:55 GMT Organization: Loyola University Chicago Lines: 1 Message-ID: <4mrpeb$qt@artemis.it.luc.edu> NNTP-Posting-Host: 147.126.1.9 NNTP-Posting-User: csriniv X-Newsreader: TIN [version 1.2 PL2] From owner-recombination@net.bio.net Wed May 08 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!carbon!night.primate.wisc.edu!sdd.hp.com!swrinde!sgigate.sgi.com!sloc223.cds.sloc.net!cdshub.cdc.com!news4.mr.net!mr.net!chi-news.cic.net!news.luc.edu!orion.it.luc.edu!csriniv From: csriniv@orion.it.luc.edu (Chandra Srinivasan) Newsgroups: bionet.molbio.recombination Subject: cv Date: 9 May 1996 03:42:06 GMT Organization: Loyola University Chicago Lines: 1 Message-ID: <4mrpie$qt@artemis.it.luc.edu> NNTP-Posting-Host: 147.126.1.9 NNTP-Posting-User: csriniv X-Newsreader: TIN [version 1.2 PL2] From owner-recombination@net.bio.net Mon May 13 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!news.compuserve.com!news.production.compuserve.com!news From: Phaedrus <75017.1050@CompuServe.COM> Newsgroups: bionet.general,bionet.molbio.recombination Subject: The Microinjection Workshop Date: 14 May 1996 03:15:36 GMT Organization: CompuServe, Inc. (1-800-689-0736) Lines: 15 Message-ID: <4n8tso$8gl$1@mhafc.production.compuserve.com> Xref: biosci bionet.general:21647 bionet.molbio.recombination:179 Hi, Just a re-post in case I missed out on some people last time. I have just started a WWW page / E-newsletter / discussion area for those interested in microinjection as it pertains to transgenic research. If you, or indeed anyone you know, is interested in thiswee nook of science, please check out my homepage. Bear in mind that I'm just trying to float this project now, and it will get better. So far I have around 20 interested folks - so I must be doing something right (I hope). The URL is as follows: http://ourworld.compuserve.com/homepages/TheBroons Thanks for takin' the time :-) Gary From owner-recombination@net.bio.net Fri May 17 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.recombination Subject: IMPORTANT - BIOSCI Fundraising Update! Date: 18 May 1996 02:00:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 154 Sender: daemon@net.bio.net Distribution: world Message-ID: <199605180900.CAA05671@net.bio.net> NNTP-Posting-Host: net.bio.net BIOSCI is about halfway to its funding goal!! I'm interrupting the usual monthly posting of the BIOSCI miniFAQ to bring you up to date on BIOSCI fundraising progress, a topic of concern to your future use of this resource. Thank you in advance for taking the time to read this message carefully. Last year we announced that BIOSCI was going to adopt the U.S. Public Broadcasting System model to fund its operations after our DOE/NSF grant runs out later this year. Unlike PBS, we are not soliciting contributions from users; we are only selling ads on our Web pages solely to cover our operating costs. Our goal is to seek sponsorships until we build up an operating reserve of about $100,000 and then cease further promotions until we need to build the reserve back up. (The accountants among our readership will be familiar with the problem of deferred revenue which we can not safely utilize until ads have been displayed for a period of time.) We are only about halfway to our funding goal and need to raise further funds to avoid having to curtail services at net.bio.net. Fundraising is time-consuming, however, and we need your help as explained further below. Our operating costs consist of our network connection, phone lines, hardware maintenance (we will be getting newer and faster hardware soon!), plus 0.7 FTE of salaries covering UNIX systems admin, technical support, quality assurance, i.e., testing, of our system, and administrative costs (such as the time it takes to actually find/write/call potential sponsors and raise money!). Although the BIOSCI staff does get compensated for a portion of the work that they do, this project has always received a lot of free after-hours and "vacation" time labor, so we hope that no one will begrudge the time that we do charge to the project to serve you. All of the three part-time staff members, Dave Mack, Julie Lawrence, and myself, have full time day jobs and families in addition to working hard to keep this service running for all of you. Julie and Dave Mack are subcontractors for BIOSCI; my time that is charged to the project defrays a portion of my regular salary instead of adding to my income. Besides having to relocate the project, we were very busy this last year building new infrastructure such as our WWW hypermail interface to the system. This was released last December along with scores of WAIS indices for the newsgroups. Virtually everything is complete, although we do continue to find and fix bugs (many through your helpful feedback!). We are still having some problems with our WAIS indexing. The archives continue to grow rapidly. We are running over 100 indexes now versus three previously and any systems crashes cause greater havoc with the indexing than before! We are still working to fix this as fast as our resources permit and appreciate your patience, but we have been able to automate a lot of the infrastructure to reduce labor as compared to past requirements. We have also implemented new software to make moderation of BIOSCI/bionet newsgroups much easier and combat the growing problem of Internet junk mail and USENET "spamming." About 20% of our groups are now moderated, many of them by the BIOSCI staff! This, for example, made a major difference last year in the quality of content in our EMPLOYMENT/bionet.jobs.offered newsgroup which many commercial concerns and recruiting firms are using **without charge** to recruit candidates for positions in the biological sciences. We are also now in a position to have sponsors for individual newsgroups as you will have noticed if you have visited http://www.bio.net/ and clicked on "Access the BIOSCI/bionet newsgroups" recently. So, how can you help?? ---------------------- As noted above it can take a lot of time to contact potential sponsors if I have to do it all myself. Our request is quite simple. You can do two important things which will take very little time for you individually. First, please use our WWW system at http://www.bio.net/ to access the archives. You can now post or reply to messages via your Web browser. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. Our hope is to quickly raise several large corporate/institutional sponsors on our heavily-used WWW locations (some stats appended below), and then end this sponsorship campaign so that our resources can continue to be used for service provision, not fundraising. Many of our specialty newsgroup WWW archives are still used by small communities of scientists (and they haven't been heavily promoted yet). While these may be valuable niche markets to some advertisers, it will generate more labor and overhead having to find these sponsors, fairly price the locations, and deal with lots of smaller sponsorships than fewer mid-to large sponsors. We are striving to keep our operation as lean and efficient as possible since we are not trying to make careers out of running BIOSCI. We are trying if at all possible to avoid the administrative overhead entailed with processing lots of small payments to reach our fundraising goals. I'd like to thank all of you for your help in advance. In helping us, you are also helping yourselves, not only in keeping this resource available for all of the both large and small research communities that we serve, but also by alleviating the need for us to go back and compete with researchers for tight grant dollars! We promised NSF when we were awarded the BIOSCI grant that we would carry out this mission to make the service self-supporting. With your help, we will succeed in continuing BIOSCI's work into its second decade. Thank you very much! Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net A list of our prime WWW sponsorship locations follow. Please contact us for further details. ---------------------------------------------------------------------- The overall BIOSCI WWW pages are currently visited by users from close to 5500 unique computer hosts per week. Web servers only log the Internet computer/host name and frequently more than one individual can connect to us from a particular host. Main home page, http://www.bio.net, visited recently by about 2100 unique hosts per week Main Newsgroups archives page, http://www.bio.net/archives.html, visited recently by about 1200 Unique hosts per week BIO-JOURNALS archive page, http://www.bio.net/BIO-JOURNALS.html, visited recently by about 1000 unique hosts per week. EMPLOYMENT archive pages: http://www.bio.net:80/hypermail/EMPLOYMENT/ and monthly header pages, visited recently by about 800 unique hosts per week. Address database search page, http://www.bio.net/addrsearch.html, visited recently by about 450 unique hosts per week. Methods newsgroup archive pages, http://www.bio.net:80/hypermail/METHDS- REAGNTS/ and monthly header pages, visited recently by about 350 unique hosts per week. Ads can also be displayed on various combinations of other BIOSCI/bionet newsgroups. Please contact us at biosci-help@net.bio.net for details. ---------------------------------------------------------------------- From owner-recombination@net.bio.net Sat May 18 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.reston.ans.net!newsfeed.internetmci.com!in2.uu.net!dziuxsolim.rutgers.edu!niflheim.rutgers.edu!not-for-mail From: meton@niflheim.rutgers.edu (Daniel Gonzalez) Newsgroups: bionet.molbio.recombination Subject: Green-Fluorescent Protein Recombinant DNA short course. Date: 19 May 1996 18:45:43 -0400 Organization: Rutgers University Lines: 45 Message-ID: <4no8an$aii@niflheim.rutgers.edu> NNTP-Posting-Host: niflheim.rutgers.edu Summary: Rutgers University short course in recombinant DNA. Keywords: GFP short course recombinant DNA biotechnology I have been asked to announce the following short course which complements our offerings in protein purification. The State University of New Jersey Rutgers Campus at New Brunswick Office of Continuing Professional Education Programs in Biotechnology, Presents: Recombinant DNA: A Six-Day, Hands-On Laboratory Course June 23 - 28, 1996 and August 18 - 23,1996 Course Description This six-day hands-on laboratory course has been a popular on-going program with the Cook College continuing education program since 1986. The course is designed to help in the conceptualization of a cloning experiment and executing it as a first step. This is followed by analysis of the cloned gene using various techniques such as expression screening, plasmid DNA isolation, restriction analysis, Southern Blotting and DNA sequencing. Finally, site-directed mutagenesis will be used to establish structure-function relationships. For the first time the course will use the now very popular Green- Fluorescent protein (GFP) gene as a model marker for gene expression. All manipulations will be conducted on this gene. This enables the participants to get a perspective of the process of gathering biological information on a single gene using various techniques. The focus will be on a problem solving approach to gain as much information concerning the gene as possible. Theoretical background and ideas for troubleshooting will be presented. For further details you can reach us, by E-mail at: meton@rci.rutgers.edu by phone at: (908) 932-9071 extension 212 by FAX at: (908) 932-8965 From owner-recombination@net.bio.net Sat May 18 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!tank.news.pipex.net!pipex!dispatch.news.demon.net!demon!scotgate.demon.co.uk From: chris@scotgate.demon.co.uk Newsgroups: bionet.molbio.recombination Subject: Funds for a Molecular Biology Undergraduate's placement? Date: Sun, 19 May 1996 14:05:12 GMT Lines: 18 Message-ID: <832514712.28026.0@scotgate.demon.co.uk> Reply-To: chris@scotgate.demon.co.uk NNTP-Posting-Host: scotgate.demon.co.uk X-NNTP-Posting-Host: scotgate.demon.co.uk X-Newsreader: IBM NewsReader/2 v1.2.5 I'm currently completing the second year of my Molecular Biology Degree course in England. I will be spending the academic year 96/97, in Spain on Industrial training, as part of my course. My question is this; I have possible placements working in Madrid, But, as I'm sure a problem everybody faces, I need funds. I will receive my grant from the local education authority, and some funds from Erasmus. But these are not really sufficient. If somebody could point me in the correct direction, towards any possible sources of funding, I would be most grateful. If this is not really a relevant post for this newgroup, I apologise in advance, and would appreciate information on a more relevant newsgroup for this question. Thanks Chris From owner-recombination@net.bio.net Sat May 18 23:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.reston.ans.net!newsfeed.internetmci.com!uwm.edu!news.cse.psu.edu!rutgers!dziuxsolim.rutgers.edu!niflheim.rutgers.edu!not-for-mail From: meton@niflheim.rutgers.edu (Daniel Gonzalez) Newsgroups: bionet.molbio.recombination Subject: Green-Fluorescent Protein Purification short course. Date: 19 May 1996 18:43:53 -0400 Organization: Rutgers University Lines: 60 Message-ID: <4no879$ag9@niflheim.rutgers.edu> NNTP-Posting-Host: niflheim.rutgers.edu Summary: Rutgers University short course in protein purification. Keywords: GFP short course protein purification biotechnology I continue to receive inquiries on our courses, we still have room for four more applicants for the June offering of the course, which is coming up in two weeks. Also, look for my announcement in this newsgroup of our short course in recombinant DNA techniques (which will use GFP for the first time). The State University of New Jersey Rutgers Campus at New Brunswick Office of Continuing Professional Education Programs in Biotechnology, Presents: Protein Purification: Isolation, Analysis, and Characterization, A Six-Day Hands-On Laboratory Course Using the remarkable Green-Fluorescent Protein (GFP), A Novel Marker For Gene Expression, as the source material June 8 -14, 1996 July 13-18, 1996 and August 10-16, 1996 More than 350 scientists from around the world have strongly recommended this intensive course as an opportunity to develop protein research and analytical skills in a retreat setting. Participants work hard, identify and solve problems in the lab and enjoy camaraderie and good food and beer with colleagues. This six-day laboratory course covers a wide variety of conventional methods for protein isolation, purification, and characterization. The course format integrates hands-on laboratory exercises with classroom lectures, demonstrations, study breaks, and short take-home assignments. A special feature of the course is that all laboratory work will be performed on the same starting sample (Aequorea GFP*), which will be purified from an exceedingly crude form to near homogeneity as judged by high performance liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric focusing, and capillary zone electrophoresis. This feature provides a continuity of purpose, integrating dozens of preparative and analytical protein techniques in a way that few competing courses can match. A problem-solving approach will be used throughout the course. Under the guidance of experienced lab instructors, participants will work in groups of three to plan their own protocols, analyze data, and interpret results. A student-teacher ratio not greater than 8:1 will be maintained and the faculty coordinators will be present throughout the course. *Note: The use of GFP from a recombinant source (E. coli) is also being used as starting material due to its popularity within the scientific community. For further details you can reach us, by E-mail at: meton@rci.rutgers.edu by phone at: (908) 932-9071 extension 212 by FAX at: (908) 932-8965 From owner-recombination@net.bio.net Mon May 20 23:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!news.sri.ucl.ac.be!news.belnet.be!news.fundp.ac.be!mickeymouse.biocell.fundp.ac.be!user From: bgillon@biocell.fundp.ac.be (Barbara GILLON) Newsgroups: bionet.molbio.recombination Subject: bFGF receptor gene Date: 21 May 1996 15:02:43 GMT Organization: F.U.N.D.P - Cellular Biochemistry Lines: 16 Message-ID: NNTP-Posting-Host: mickeymouse.biocell.fundp.ac.be I am looking for a plasmid coding for the human recpetor of the bFGF gene. Does anyone have this plasmid? Please answer me by e-mail : bgillon@biocell.fundp.ac.be Thank you by advance, Barbara Gillon -- Barbara GILLON Laboratory of Cellular Biochemistry, Facultes Universitaires ND de la Paix, 61, rue de Bruxelles, B-5000 Namur (Belgium). Fax: ++/32/81/72.41.35. From owner-recombination@net.bio.net Tue May 21 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: CANADA: AVAILABLE IMMEDIATELY: POSTDOC/PHD anticancer efficacy of new anti-angiogenic drugs in animal Date: 22 May 1996 05:36:30 -0700 Organization: McGill Div. of Experimental Medicine Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: <31A3077F.45E9@odyssee.net> NNTP-Posting-Host: net.bio.net Job Listing Anticancer efficacy of new anti-angiogenic drugs in animal models Dr. Moulay Alaoui-Jamali McGill University Montreal, Quebec, Canada Project description: Financial support is currently available to conduct a study on the anticancer efficacy of new anti-angiogenic drugs in animal models. AVAILABLE IMMEDIATELY Positions: 1 Position (Ph.D./Post Doc) Qualifications: Previous experience with animal models To Apply: Please send your CV/contact immediately to: Dr. Moulay Alaoui-Jamali Tel. 514-340-8260 Ext. 3438 Fax 514-340-7576. E. mail: MDAJ@MUSICA.McGILL.CA From owner-recombination@net.bio.net Tue May 21 23:00:00 1996 Path: biosci!musica.mcgill.ca!axsd From: axsd@musica.mcgill.ca (Sylvain Dancausse) Newsgroups: bionet.molbio.recombination Subject: Quality of life after Myocardial Infarction/ Research Nurse Coord/ Montreal Canada Date: 22 May 1996 14:36:43 -0700 Organization: Montreal General Hospital Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: <31A3884B.5FEB@musica.mcgill.ca> NNTP-Posting-Host: net.bio.net Position : Research Nurse Coordinator Contact : Louise Pilote, MD MPH Clinical Epidemiology, Montreal General Hospital, Montreal, Quebec Project description: A multicenter study based at the Montreal General Hospital aimed at measuring quality of life after acute myocardial infarction. Positions: Research Nurse Coordinator Qualifications: Nurse Experienced in project administration including supervisory experience Excellent personal, organizational and writing skills Fluently bilingual Job Description: Part-time job Supervise data collection Visit to study sites to identify study patients, administer questionnaire and do chart review Organise mail survey Participate in data entry,analysis of data and report of study results To Apply: Send CV by July 1st to: Louise Pilote, MD MPH Montreal General Hospital, L-10, 416, 1650 Cedar, Montreal, Quebec, H3G 1A4. Fax: 934-8293, E-mail: mdlp@musica.mcgill.ca From owner-recombination@net.bio.net Tue May 21 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: CANADA-PHD JOB: MONTREAL (Differentiation and Apoptosis of Skeletal cells) Date: 21 May 1996 21:35:08 -0700 Organization: McGill Div. of Experimental Medicine Lines: 64 Sender: daemon@net.bio.net Distribution: world Message-ID: <31A296B3.5BE7@odyssee.net> NNTP-Posting-Host: net.bio.net DO NOT REPLY VIA NEWS READER OR E-MAIL! *************************************** ONLY CV's RECIEVED VIA FAX OR MAIL WILL BE CONSIDERED. DEADLINE May 31/96 Job Listing Characterization of molecular mechanisms involved in differentiation and apoptotic death of skeletal cells. Janet E. Henderson, Ph.D. Lady Davis Institute for Medical Research Montreal, Quebec, Canada Project description: Characterization of molectular mechanisms involved in differentiation and apoptotic death of skeletal cells Ongoing research in this laboratory is focused on understanding the integrated mechanism of action of peptide growth factors, such as FGF and PTHrP, during skeletogenesis. More Specifically, this project is aimed at characterizing signalling pathways involved in chodrocyte differentiation and programmed cell death using an in vitro model of chondrogenesis. The successful candidate will gain expertise in a wide variety of cellular and molecular biological techniques while working on a project relevant to diseases of bone and mineral metabolism. Positions: 1 Ph.D. Position Funding is available for a Ph.D. student interested in pursuing studies related in bone cell biology. Prospective candidates must hold a B.Sc. degree in the biological sciences and should have a GPA of 3.4 or higher. DEADLINE MAY 31st, 1996 To Apply: Forward copies of curriculum vitae and academic trascripts no later than May 31 Dr. Janet E. Henderson, Lady Davis Institute for Medical Research, 3755 Ch. Cote Ste-Catherine, Montreal, Quebec, Canada. H3T 1E2. Fax: (514) 340 7573 *Include your telephone, Fax and e-mail numbers. From owner-recombination@net.bio.net Sun May 26 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: Current Biology...How does it rate in the citation Index? Date: 27 May 1996 14:27:06 -0700 Organization: McGill Div. of Experimental Medicine Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: <31AA1B55.5F2C@odyssee.net> NNTP-Posting-Host: net.bio.net Hello, I was wondering how highly ranked Current Biology is in the citation index? Does anyone have access to this? Were can I find out the ranking for different journals?? I got a subscription to this journal and I find it and Current Opinions Journals are usually quite good reading, better than Science and Natures condensed review articles but less than Cells.... but covering more subjects. G. Dellaire Experimental Medicine McGill University. dellaire@odyssee.net From owner-recombination@net.bio.net Sun May 26 23:00:00 1996 Path: biosci!bdg10.niddk.nih.gov!KAYUWEW From: KAYUWEW@bdg10.niddk.nih.gov ("WAGNER, KAY-UWE") Newsgroups: bionet.molbio.recombination Subject: Adenovirus vectors Date: 27 May 1996 16:15:00 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 7 Sender: daemon@net.bio.net Distribution: world Message-ID: <31AA36A7@SMTP2.mm.hub.nih.gov> NNTP-Posting-Host: net.bio.net Does anybody know a good company which is developing/making adenovirus vectors? Thanks in advance. Kay-Uwe Wagner kayuwew@bdg10.niddk.nih.gov From owner-recombination@net.bio.net Wed May 29 23:00:00 1996 Path: biosci!rutgers!sgigate.sgi.com!swrinde!newsfeed.internetmci.com!news.internetMCI.com!darwin.sura.net!spitfire.msrcnavo.navy.mil!NewsWatcher!user From: sbaggett@wpogate.ssc.nasa.gov Newsgroups: bionet.molbio.recombination Subject: information Date: 30 May 1996 16:24:02 GMT Organization: NAVOCEANO Lines: 4 Message-ID: NNTP-Posting-Host: 148.114.73.221 I am a student in a biotechnology program leading to an associates degree in applied science. I am requesting information about fields of employment and pay ranges. If anyone has any information please Email me at smbtibble@aol.com. Thank You