From owner-recombination@net.bio.net Tue Oct 01 23:00:00 1996 Path: biosci!daresbury!is.bbsrc.ac.uk!news From: Mick Partis Newsgroups: bionet.general,bionet.molbio.recombination,bionet.plants Subject: Re: ANNOUNCE: Plasmid Processor software available for free!---MAC please??? Date: 2 Oct 1996 11:17:07 GMT Organization: Horticulture Research International Lines: 23 Message-ID: <52tivj$ut8@is.bbsrc.ac.uk> References: <324F231C.716F@post.drexel.edu> NNTP-Posting-Host: pc0233.hriw.bbsrc.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (Windows; I; 16bit) Xref: biosci bionet.general:23434 bionet.molbio.recombination:283 bionet.plants:12749 Buddha wrote: >Hi, > >Would you happened to have developed such a program for the Macintosh? >The program sounds very useful for us, but our lab functions mainly on >the Mac/Power PC systems. > > Thanks, > > Buddha There is a plasmid drawing program (amongst all of the other mac software) called macplasmap downloadable from gopher://ftp.bio.indiana.edu:70/11/IUBio-Software+Data/molbio/mac Mick -- mick.partis@hri.ac.uk Horticulture Research International http://www.geocities.com/CapeCanaveral/1957/ From owner-recombination@net.bio.net Wed Oct 09 23:00:00 1996 Path: biosci!agate!spool.mu.edu!howland.erols.net!swrinde!news-peer.gsl.net!news.gsl.net!news-penn.gsl.net!news.gsl.net!snunews.snu.ac.kr!usenet From: pharmnmr Newsgroups: bionet.cellbiol,bionet.microbiology,bionet.molbio.recombination Subject: Some terms on plasmid replication control? Date: Thu, 10 Oct 1996 21:55:15 -0700 Organization: College of Pharmacy, Seoul Nat'l Univ. Lines: 13 Message-ID: <325DD333.167E@pharmnmr.snu.ac.kr> NNTP-Posting-Host: pharmnmr.snu.ac.kr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.3 IP22) Xref: biosci bionet.cellbiol:5648 bionet.microbiology:7451 bionet.molbio.recombination:286 I'm trying to study plasmid replication control. While I was reading a paper, I met some biological terms unfamiliar to me. plus origin of replication minus origin of replication countertranscript RNA(ctRNA) - Is it different from antisense RNA? Could you tell me meaning of above terms or references on plasmid replication mechanism & control? Additionally I'll appreciate to you if you tell me what news groups are involved with this subject. Thank you. From owner-recombination@net.bio.net Sat Oct 12 23:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: News Group for Genome Structure/function analysis Date: 12 Oct 1996 20:50:15 -0700 Organization: McGill Div. of Experimental Medicine Lines: 119 Sender: daemon@net.bio.net Distribution: world Message-ID: <32610ED5.401C@odyssee.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net Dear Bionet readers, I am posting this note to ask for comments and suggestions for a new news group on genome/chromatin structure and function: tentatively called bionet.genome.structure The importance of chromatin/genomes structure for the processes of replication, transcription and recombination is becoming more and more apparent. Chromatin context can affects the expression and replication timing of a gene domain. Such interelationship between gene function and changes in chromatin structure have been demonstrated through an evolution of techniques from Dnase I sensitivity mapping to fluorescent in situ hybridization (FISH. I believe with such far reaching affects on many areas of molecular biology, it is time we devoted a news group to the study of genome structure and function. The following is a list of "possible" topics. If you feel we should include others or have suggestions as to the format of the news group please reply via e-mail to: dellaire@odyssee.net In addition to myself three tentative discussion leaders have already been contacted and wish to encourage the formation of such a group. They are: Dr. Eric Milot (Erasmus, Neatherlands) Dr. Ronald Hancock (U of Laval, Quebec, Canada) Dr. Peter Cook (Oxford, England) Here is the list of topics so far. 1. Genome/chromatin accessibility and recombination -recombination hotspots (mieotic and mitotic) -fragile sites -imprinting and recombination rates -ectopic gene targeting and chromatin structure 3. Effect of DNA topology/structure(Triple strand, Z-DNA, cruciform, bent etc) on biological processes such as: -replication -transcription -recombination 4. Histones and Nucleosomes and chromatin structure/function -H1 repression of transcription -Post translational modification of histones acetylation (H4, H3), phosphorylation (H1, H3) and ubiquitination (H2A, H2B) -Histone variants (ex. H2A.Z in mammals, H5 of chicken) 5. Models of genome structure (Loop Domain Model, Channel Model, MegaBase Giant Loop Model, etc.) 6. Evolution of the Genome -isochores and base-content (GC vs. AT) -formation of gene clusters and syntenic mapping -repetitive elements (satellites, telomeric and centromeric (alpha) repeats, lines and sines) 7. Biologically important mutants and knockouts that affect genome/chromatin structure -ex. SNF/SWI, TOPO mutants in yeast -RAD 51,52,54 knockout mice -AT, BLM, FA mouse models 8. Techniques for genome/chromatin analysis -Fluorescent Insitu Analysis -psoralen, polyamine crosslinking -In vivo nucleosome foot printing -Dnase I/Micrococcal Nuclease sensitivity -VM26 Topoisomerase II site mapping 9. Chromatin/DNA binding proteins and their effects on chromatin structure and/or gene expression -Polycomb proteins -Rap1 (telomere silencing) -alpha2-MCM1 (repression of MAT locus) -CENP A/B/C (centromere structure/function) -XCAP-C/E, SMC1/2 (chromatin Condensation) -remodeling of chromatin by SWI/SNF proteins 10. Matrix attatchent regions (MAR's), domain boundaries and locus control regions (LCR's) and their relationship to gene structure and function. -definition of transcription/replication domains -model systems ex. betaglobin (LCR) SCS/SCS' of the Drosophila Heat Shock Locus (HS87a7) 11. Phenomenon of Position Effect and Transvection -in drosophila (HP1, polycomb, heterochromatization) -in mammalian systems (silencing or variegated expression of transgenes) 12. Epigenetic effects on gene function -imprinting -methylation -maintenance of early/late replication 13. Dosage compensation mechanisms and X chromosome inactivation -MSL proteins of Drosophila -XIC (Xist RNA) in mammals -CpG methylation 14. Chromatin structure and DNA replication -ORC1 protein of yeast 15. DNA repair and chromatin structure -TFIIH (transcription coupled repair) -p53 -BLM and AT genes -poly-ADP-polymerase (PARP) From owner-recombination@net.bio.net Thu Oct 17 23:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.recombination Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 18 Oct 1996 02:00:47 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199610180900.CAA20975@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-recombination@net.bio.net Thu Oct 17 23:00:00 1996 Path: biosci!rutgers!news.sgi.com!howland.erols.net!www.nntp.primenet.com!nntp.primenet.com!nntp.uio.no!nntp.zit.th-darmstadt.de!fu-berlin.de!uniol!news.uni-stuttgart.de!news From: "Igor M. Kulic" Newsgroups: bionet.molbio.recombination Subject: LOOKING FOR TOPOISOMERASE AND INTEGRASE Date: Fri, 18 Oct 1996 13:44:29 +0200 Organization: UNI-Stuttgart Lines: 11 Message-ID: <32676D9D.41C6@cip.mathematik.uni-stuttgart.de> NNTP-Posting-Host: cip13.mathematik.uni-stuttgart.de Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; AIX 2) CC: bionet.microbiology Dear sirs , does anybody of you know where it is possible to get any kind of TOPOISOMERASE (type I and II) and INTEGRASE (-Lambda for instace) .Is there anybody out there who could borrow or sell us (biochemistry department Stuttgart -Germany) some small quantities of these two enzymes that are necessery for our further research ? We got some difficulties in finding any comercial company that offers them . Thanks in advance , kindly yours Igor Kulic,Stuttgart(Germany) From owner-recombination@net.bio.net Sun Oct 20 23:00:00 1996 Path: biosci!IMMAG.MCG.EDU!moshe From: moshe@IMMAG.MCG.EDU (Moshe Sadofsky) Newsgroups: bionet.molbio.recombination Subject: Post-Doc position in V(D)J recombination available Date: 21 Oct 1996 08:09:48 -0700 Organization: MCG Lines: 28 Sender: daemon@net.bio.net Distribution: world Message-ID: <326B927D.49E6@immag.mcg.edu> Reply-To: moshe@immag.mcg.edu NNTP-Posting-Host: net.bio.net Moshe Sadofsky is setting up a new lab at the brand-new Institute of Molecular Medicine and Genetics at the Medical College of Georgia, Augusta Ga. I need one more post-doctoral researcher for continuing studies of the RAG genes and the molecular biology of this recombination system. See the group advertisement in Science 273 July 5, 1996, p. 144. We will study aspects of the DNA rearrangements that occur in the immune system at the cellular and cell-free level. Currently, I am developing methods of mapping the sites of interaction of the RAG proteins with the recombination signal. This will provide insight into the functional role of these proteins in the reaction, and will identify the binding and enzymatic active sites on the proteins. An especially interesting question is how the two types of signal sequences (with 12- and 23- base pair spacers) are distinguished from each other and possibly cooperate to specify unique cut sites within a larger array. The connection between DNA recombination and double strand break repair is also of high interest. Please pass this information along to potential candidates. CONTACT:Moshe Sadofsky MCG-IMMAG CB-2803 Augusta GA 30912-2650 e-mail: moshe@immag.mcg.edu Phone : 706 721-8761 Fax number: 706 721-8752 From owner-recombination@net.bio.net Sun Oct 20 23:00:00 1996 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!www.nntp.primenet.com!nntp.primenet.com!howland.erols.net!quagga.ru.ac.za!ru.uni.net.za!uct.uni.net.za!iafrica.com!not-for-mail From: "Johan" Newsgroups: bionet.molbio.recombination Subject: Blunt-end ligation Date: 21 Oct 1996 21:18:05 GMT Organization: Internet Africa (UUNET) Lines: 9 Message-ID: <01bbbf95$1362e060$96ab07c4@master> NNTP-Posting-Host: 196-7-171-150.iafrica.com X-Newsreader: Microsoft Internet News 4.70.1155 After several efforts to ligate adapters to blunt-ended resriction fragments, I have failed to do complete this sucessfully and require information regarding optimal ligation protocols for the construction of a genomic patato library. Any advice would be greatly appreciated. Regards Johan : jwbark@iafrica.com From owner-recombination@net.bio.net Tue Oct 22 23:00:00 1996 Path: biosci!ORIGIN.GIG.USDA.GOV!srheller From: srheller@ORIGIN.GIG.USDA.GOV ("Stephen R. Heller") Newsgroups: bionet.molbio.recombination Subject: Plant & Animal Genome V Conference Date: 22 Oct 1996 21:19:31 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net This is an announcment and a reminder that Plant & Animal Genome V will be held this January 12-16, 1997 in San Diego. The deadline for submission of posters is 4 November. Pre-registration deadline is 15 November. For complete program details, including some two dozen+ owrkshops, please point your browser to: http://pgenome.arsusda.gov:8000/pag5draft.html Steve Heller, USDA, ARS, Plant Genome Project Bldg. 005, Room 337 Beltsville, MD 20705-2350 USA Phone: 301-504-6055 FAX: 301-504-6231 E-mail: srheller@gig.usda.gov WWW: www.hellers.com/~steve From owner-recombination@net.bio.net Wed Oct 23 23:00:00 1996 Path: biosci!bloom-beacon.mit.edu!eru.mt.luth.se!news.algonet.se!news.uoregon.edu!hammer.uoregon.edu!hunter.premier.net!feed1.news.erols.com!howland.erols.net!vixen.cso.uiuc.edu!rhizox2.ppath.uiuc.edu!g-yu1 From: g-yu1@uiuc.edu Newsgroups: bionet.molbio.recombination Subject: small peptide help Date: Thu, 24 Oct 1996 18:14:05 CST Organization: University of Illinois at Urbana Champaign Lines: 25 Message-ID: NNTP-Posting-Host: rhizoc2.ppath.uiuc.edu X-Newsreader: Trumpet for Windows [Version 1.0 Rev B] Hi Dear everyone: Please help me. I purified a small peptide (Mr 1050 by mass spetrometry) from a Bacillus culture by reverse chromatography. AA composition analysis result says it has only five to seven kinds of AA(Asx, Glx, Ser, Tyr, Pro). We were unable to get any sequence information by Edman degregation method. We believe it is cyclic or N-terminal blocked. My questions are: 1> If it is cyclic, and if I run SDS-page or do gel-filtration chromatography, Does it behave the same way as those linear peptides having same MW? 2> How can I figure out such a small(MW 1050) peptide's pI? Is IEF gel ok? 3> Since many peptides produced by this kind of soil bacteria are non-ribosomally synthesized, how can I know if it is ribosomally synthesized or non-ribosomally synthesized on a protein-template? I appreciate any suggestion very much. ********************************************* George Y. Yu Graduate Student University of Illinois at Urbana-Champaign Ph: 217-367-1969 email: g-yu1@uiuc.edu ********************************************* From owner-recombination@net.bio.net Thu Oct 24 23:00:00 1996 Path: biosci!rutgers!news.sgi.com!mr.net!www.nntp.primenet.com!nntp.primenet.com!feed1.news.erols.com!howland.erols.net!vixen.cso.uiuc.edu!ux1.cso.uiuc.edu!mvidakov From: vidakovic momcilo Newsgroups: bionet.molbio.recombination Subject: Megaprimer PCR Date: Thu, 24 Oct 1996 22:46:00 -0500 Organization: University of Illinois at Urbana Lines: 19 Message-ID: NNTP-Posting-Host: ux1.cso.uiuc.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII I've been trying to run megaprimer PCR and I've had success in amplifying the gene of interest with 220 bp primer. Unfortunately, the sequence of the amplified gene carries T -> A unwanted mutation in the region that overlaps 3'-end of the megaprimer. My question is: is there anyway to control the 3' non-template dependent base addition/mutation? Any help would be greatly appreciated. Thanks, Moma (mvidakov@ux1.cso.uiuc.edu) From owner-recombination@net.bio.net Thu Oct 24 23:00:00 1996 Path: biosci!JUSTINE.UMontreal.CA!assad From: assad@JUSTINE.UMontreal.CA (Michel Assad) Newsgroups: bionet.molbio.recombination Subject: APOPTOSIS Date: 25 Oct 1996 09:57:56 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I have seen in the litterature that methods that are able to identify DNA damage may also be able to identify apoptosis. Also, authors generally consider genotoxicity as an initial step towards carcinogenicity. The problem is that apoptosis seems to be the way nature counterbalances carcinogenesis. In general terms, cancer is the opposite of apoptosis! How would you then correlate apoptosis, carcinogenicity and genotoxicity? Michel. ================================================================================ * Michel Assad * Ste-Justine Hospital * 3175, Cote Ste-Catherine * Montreal (Quebec) H3T 1C5 * CANADA * FAX: 1 (514) 345-4723 * E-MAIL: assad@justine.umontreal.ca ================================================================================ From owner-recombination@net.bio.net Mon Oct 28 22:00:00 1996 Path: biosci!agate!howland.erols.net!EU.net!news.sprintlink.net!news-peer.sprintlink.net!arclight.uoregon.edu!news.uoregon.edu!newsfeed.orst.edu!news.orst.edu!sslab.FSL.ORST.EDU!krutovsk From: krutovsk@fsl.orst.edu (Konstantin Krutovskii) Newsgroups: bionet.molbio.recombination Subject: PAGE equipment to do SSCP Date: Mon, 28 Oct 1996 16:12:22 Organization: Forestry Sciences Lab Lines: 23 Message-ID: NNTP-Posting-Host: sslab.fsl.orst.edu Keywords: PAGE , PCR, SSCP (single strand conformational polymorphism) X-Newsreader: Trumpet for Windows [Version 1.0 Rev A] Dear netters, I am looking for good, reliable and easy to assemble PAGE equipment and accessories to do SSCP (single strand conformational polymorphism) analysis. I would appreciate it very much if you could name the best manufacturer or company in terms of price and quality. I would also appreciate all advice on the gel system, size / length of gel, optimal number of samples to run, etc., as well as any hints on buffer and gel contents (for instance, acrylamide/bis ratio), and on staining systems (silver vs. radiolabeling vs. fluorescent). I have found that many researchers use Protein PAGE apparatus instead DNA sequencing systems to separate PCR-SSCP products. They are shorter and thicker, but may be easier to assemble. Do they give resolution comparable with sequencing gel? I also wonder if there is any web site where I can inquire about SSCP. Sincerely, Konstantin krutovsk@ccmail.orst.edu From owner-recombination@net.bio.net Wed Oct 30 22:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: TopoII and Integrase... Date: 31 Oct 1996 06:19:24 -0800 Organization: McGill Div. of Experimental Medicine Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <32795C8E.2D39@odyssee.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net Hello Igor, I saw your post and although I haven'thad time to check it out. I think you can get Topo II from Borringer Manheim, or GibcoBRL. In fact maybe even Pharmacia. Integrase may be harder to find. You might have to get the cDNA from a lab that works with the enzyme and the recombination system. Graham From owner-recombination@net.bio.net Thu Oct 31 22:00:00 1996 Path: biosci!OCELOT.RUTGERS.EDU!gabriel From: gabriel@OCELOT.RUTGERS.EDU ("gabriel,abram") Newsgroups: bionet.molbio.recombination Subject: postdoctoral position available to study nonhomologous recombination and transposition Date: 1 Nov 1996 13:06:29 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 37 Sender: daemon@net.bio.net Distribution: world Message-ID: <327A7599.8E9@mbcl.rutgers.edu> NNTP-Posting-Host: net.bio.net Post-doctoral research position available, in the laboratory of Dr. Abram Gabriel at Rutgers University in Piscataway, New Jersey. We study a variety of questions related to retrotransposon reverse transcriptases. These include: 1)Defining the connection between retrotransposon reverse transcriptases (yeast Ty1, humasn L1, and Crithidia CRE1) and double-strand break repair in the yeast Saccharomyces cerevisiae (see Teng, S. C., Kim, B., and Gabriel, A. Retrotransposon reverse transcriptase-mediated repair of chromosomal breaks, Nature, 383, 641-644, 1996) 2)Studying the molecular determinants of reverse transcriptase fidelity in yeast (see Gabriel, A., Willems, M., Mules, E.H., and Boeke, J.D. Replication infidelity during a single cycle of Ty1 retrotransposition, Proceedings of the National Academy of Science, USA, 93, 7767 7771, 1996). 3)Examining the replication mechanisms of trypanosome site-specific non-LTR retrotransposons (see Teng, S.C., Wang, X., and Gabriel, A. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays, Nucleic Acids Research, 23, 2929-2936, 1995; Interested applicants should contact: Abram Gabriel Dept. of Molecular Biology and Biochemistry Rutgers University CABM 306 679 Hoes Lane Piscataway, NJ 08855 USA 908-235-5097(phone) 908-235-4880(fax) gabriel@mbcl.rutgers.edu From owner-recombination@net.bio.net Thu Oct 31 22:00:00 1996 Path: biosci!agate!howland.erols.net!wits.uni.net.za!csir.uni.net.za!news.mikom.csir.co.za!news.up.ac.za!scientia.up.ac.za!julian From: julian@scientia.up.ac.za Newsgroups: bionet.molbio.recombination Subject: Bacillus integration vector Date: Fri, 1 Nov 1996 10:52:57 GMT Organization: University of Pretoria Lines: 6 Message-ID: NNTP-Posting-Host: 137.215.27.245 Hi Does anyone know of a commercially available Bacillus integration vector? Thanks, Jonathan. From owner-recombination@net.bio.net Thu Oct 31 22:00:00 1996 Path: biosci!rutgers!news.sgi.com!howland.erols.net!wits.uni.net.za!csir.uni.net.za!news.mikom.csir.co.za!news.up.ac.za!scientia.up.ac.za!julian From: julian@scientia.up.ac.za Newsgroups: bionet.molbio.recombination Subject: test - ignore Date: Fri, 1 Nov 1996 10:51:30 GMT Organization: University of Pretoria Lines: 1 Message-ID: NNTP-Posting-Host: 137.215.27.245 Guess you didn't!!!