From owner-recombination@net.bio.net Tue Nov 05 22:00:00 1996 Path: biosci!fcs280s.ncifcrf.gov!cpk-news-feed1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!newsfeed.internetmci.com!in1.uu.net!newsflash.concordia.ca!news.mcgill.ca!news From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: Call For Discussion: New News Group Proposal On Gene Structure and Function Date: 4 Nov 1996 16:15:47 GMT Organization: McGill University Computing Centre Lines: 287 Message-ID: <55l4rj$kki@sifon.cc.mcgill.ca> NNTP-Posting-Host: b-11.das.mcgill.ca X-Newsreader: AIR News 3.X (SPRY, Inc.) We have received a proposal below for a new newsgroup, GENSTRUCTURE/bionet.genome.gene-structure (moderated) Discussion on the following proposal will now be open through 10 Nov on BIOFORUM/bionet.general (*not* on BIONEWS/bionet.announce). For those who are not on the BIOFORUM/bionet.general newsgroup currently, either read bionet.general on USENET or contact one of the following biosci addresses to sign up by e-mail: Subscription Address Location -------------------- -------- biosci@daresbury.ac.uk Europe, Africa, and Central Asia biosci@net.bio.net Americas and the Pacific Rim One warning, however: BIOFORUM/bionet.general is a "chatty" newsgroup and many other items will be discussed there besides this newsgroup proposal. USENET news access is definitely better than e-mail if you want to monitor only this discussion. Discussion Guidelines --------------------- Please do not post one-line messages saying things like "I am in favor of such a newsgroup". We will collect votes later. The discussion period is an opportunity for anyone to bring up reservations about the proposed charter below and to propose modifications prior to the vote. If the charter is perfect as is, then there is no need for anyone to say anything!!! The newsgroup discussion leader may submit a revised proposal in light of the discussion, and the new charter would be included in the call for votes which will go out on 11 November (Newsgroup Discussion leaders - please note this deadline - if no revisions are received before the deadline, the original charter will be voted on.). Please be aware that only one vote is counted per e-mail address, so it is necessary to have your own account in order to vote. Multiple votes from the same address are not accepted. If you are interested in the following newsgroup, but do not have an e-mail address of your own, please obtain one from your computer center before the call for votes is issued. --------------------------------------------------------------------- Proposal for GENSTRUCTURE/bionet.genome.gene-structure (moderated) Proposed USENET name: bionet.genome.gene-structure One line Description: Genome and chromatin structure and function Status: Moderated Proposed Moderation address: bionet-genome-gene-structure@net.bio.net (genstruc-moderator@net.bio.net is an alias for bionet-genome-gene-structure@net.bio.net) Moderator: Graham Dellaire Proposed mailing list name: GENSTRUCTURE Proposed e-mail addresses: genstruc@net.bio.net genstruc@daresbury.ac.uk Charter: The purpose of the GENSTRUCTURE newsgroup is to provide a proper forum for the discussion of issues pertaining and involving genome and/or chromatin structure and function (see _Topics of Discussion_). Primarily it should enable those researchers who work in genome/chromatin structure or related fields to communicate ideas and information, as well as, provide a chance for collaboration among national and international research groups. --------------------------------------------------------------------- Topics of Discussion include: 1. Genome/chromatin accessibility and recombination -recombination hotspots (mieotic and mitotic) -fragile sites -imprinting and recombination rates -ectopic gene targeting and chromatin structure 2. 3D-organization of the nucleus -chromosome territories -nucleoli -nuclear lamina (telomere localization) -tissue or cell cycle dependent positioning of chromosomes -RNA tracking 3. Effect of Superhelicity and DNA topology/structure(Triple strand, Z-DNA, cruciform, bent etc) on biological processes such as: -replication (ex. replication fork barriers, initiation sites) -transcription (ex. promoter function in relation to superhelicity) -recombination (ex. Gin and Hin invertases and superhelicity) 4. Histones and Nucleosomes and chromatin structure/function -H1 repression of transcription -Post translational modification of histones acetylation (H4, H3), phosphorylation (H1, H3) and ubiquitination (H2A, H2B) -Histone variants (ex. H2A.Z in mammals, H5 of chicken) 5. Models of genome structure (Loop Domain Model, Channel Model, MegaBase Giant Loop Model, etc.) 6. Classical chromosome elements and their relationship to gene function -centromeres (constitutive heterochromatin and gene silencing) -telomeres (associated silencing and involvement in position effect) 7. Evolution of the Genome -isochores and base-content (GC vs. AT) -formation of gene clusters and syntenic mapping -repetitive elements (satellites, telomeric and centromeric (alpha) repeats, lines and sines) 8. Biologically important mutants and knockouts that affect genome/chromatin structure -ex. SNF/SWI, TOPO mutants in yeast -RAD 51,52,54 knockout mice -AT, BLM, FA mouse models 9. Techniques for genome/chromatin analysis -cytogenetic techniques (g banding, r and q) -Fluorescent In situ analysis(FISH) -Confocal microscopy -Electron microscopy -Electron microscopy In situ analysis (EMISH) -psoralen, polyamine crosslinking -In vivo nucleosome foot printing -Dnase I/Micrococcal Nuclease sensitivity -VM26 Topoisomerase II site mapping 10. Chromatin/DNA binding proteins and their effects on chromatin structure and/or gene expression -Polycomb proteins -Rap1 (telomere silencing) -alpha2-MCM1 (repression of MAT locus) -CENP A/B/C (centromere structure/function) -XCAP-C/E, SMC1/2 (chromatin Condensation) -remodeling of chromatin by SWI/SNF proteins -H-NS in bacteria (role in nucleoid topology vs. gene function) 11. Nuclear Matrix (NM) and Nuclear Lamina (NL) -cell cycle regulation of formation of NM and NL -transcription and replications factors and the concept of a dynamic NM (ex. tissue and temporal specificity) -role of NM in signal transduction (mechanistic vs. chemical signals) 12. Matrix attatchent regions (MAR's), domain boundaries and locus control regions (LCR's) and their relationship to gene structure and function. -definition of transcription/replication domains -model systems ex. betaglobin (LCR) SCS/SCS' of the Drosophila Heat Shock Locus (HS87a7) 13. Phenomenon of Position Effect and Transvection -in drosophila (HP1, polycomb, heterochromatization) -in mammalian systems (silencing or variegated expression of transgenes) -in fungi 14. Epigenetic effects on gene function -imprinting -methylation -maintenance of early/late replication 15. Dosage compensation mechanisms and X chromosome inactivation -MSL proteins of Drosophila -XIC (Xist RNA) in mammals -CpG methylation 16. Chromatin structure and DNA replication -ORC1 protein of yeast -origins of replication (association with cruciform, bent DNA and MARS) 17. Specialized methods of nuclear packaging -bacterial nucleoid -packaging of DNA in spermatozoa 18. DNA repair and chromatin structure -TFIIH (transcription coupled repair) -p53 -BLM and AT genes -poly-ADP-polymerase (PARP) 19. Mechanisms of genomic instability -during oncogenesis -process of chromosomal aberration (stable and unstable) (ex. role of micronuclei in the process) -chromosomal aberration during aging and mechanisms of instability (ex. telomeres and telomerase) In addition this newsgroup provides: A forum for the exchange of information about future congresses and meetings in areas of molecular biology relating to genome structure/function. A forum for the exchange of information about textbooks, internet resources, visual materials, and computer programs. A source of quick help for last-minute troubleshooting, sources of materials, and practical advice; in areas such as -Specific common resources (ex. primers, antibodies, vectors) -Genome analysis techniques -Transgenic models and mutant cell lines Moderation Policy: Mass-posted commercial messages, chain letters, and similar postings not associated with or pertaining to the study of genome/chromatin structure and function will be deleted without comment. Inappropriate messages posted in good faith will be returned to the sender. Messages not strictly within the charter but likely to be of interest to many subscribers (e.g., messages dealing with transcription and replication factors) will be accepted. Subscribers are welcome from universities or any academic institutions, government agencies, medical institutions, and industrial or commercial organizations. Contributions within the functions outlined above are encouraged. Proposed discussion leaders: *Note: Area of expertise is written in brackets Graham Dellaire, e-mail: dellaire@odyssee.net (gdella@po-box.mcgill.ca) Institut du Cancer de Montreal Centre de Recherche L.C. Simard 1560 Sherbrooke Street East, Montreal, Quebec, Canada H2L 4M1 telephone:1 (514) 876 6936; fax: (514) 876 5476 *(Expertise:chromatin structure and recombination; Recombination Access Mapping (RAM); genome structure analysis) Ronald Hancock, e-mail: ronald.hancock@crhdq.ulaval.ca Professor Laval University Cancer Research Centre 1 rue de l'Arsenal Quebec, Canada G1R 2J6 telephone: 1 (418) 691-5281; fax: 1 (418) 691-5439 *(Expertise:genome organisation in vivo; models of genome structure; topoisomerases; VM26 topoisomerase II site mapping) Eric Milot, e-mail: milot@ch1.fgg.eur.nl Faculty of Medicine Dept. of Cell Biology and Genetics P.O. Box 1738 3000 DR Rotterdam The Netherlands telephone: 3110 4087164; fax: 3110 4360225 *(Expertise:Chromatin context and transcription; Polycomb protein; position effect variegation; chromosome and nuclear organisation; dosage compensation) **Tentative Discussion leader** Peter Cook, e-mail: peter.cook@pathology.oxford.ac.uk Professor of Cell Biology, The Sir William Dunn School of Pathology, South Parks Road, Oxford, OX1 3RE, UK. Telephone (direct line) : +44/0 1865 275528 Telephone (switchboard) : +44/0 1865 275500 Fax : +44/0 1865 275501 http://www.molbiol.ox.ac.uk/www/users/counsell/cook.htm (Expertise:Chromatin/Genome Structure and Function in regard to transcription and replication) ---------------------------------------------------------------------- From owner-recombination@net.bio.net Tue Nov 12 22:00:00 1996 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: CALL VOTES: GENSTRUCTURE NEWS GROUP-bionet.genome.gene-structure Date: 13 Nov 1996 05:42:16 -0800 Organization: McGill Div. of Experimental Medicine Lines: 298 Sender: daemon@net.bio.net Distribution: world Message-ID: <3289D0B3.6262@odyssee.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net Voting is now open on the following proposal to create the mailing list & newsgroup GENSTRUCTURE/bionet.genome.gene-structure (moderated) The charter was not modified as a result of the discussion. *** NOTE *** We are often running several votes for other newsgroups, so please be certain to follow the voting directions *carefully*! If you just send in a message saying "YES" or "NO" it will not be counted if it is not clear which proposal you are responding to. ---------------------------------------------------------------------- Proposal for GENSTRUCTURE/bionet.genome.gene-structure (moderated) Proposed USENET name: bionet.genome.gene-structure One line Description: Genome and chromatin structure and function Status: Moderated Proposed Moderation address: bionet-genome-gene-structure@net.bio.net (genstruc-moderator@net.bio.net is an alias for bionet-genome-gene-structure@net.bio.net) Moderator: Graham Dellaire Proposed mailing list name: GENSTRUCTURE Proposed e-mail addresses: genstruc@net.bio.net genstruc@daresbury.ac.uk Charter: The purpose of the GENSTRUCTURE newsgroup is to provide a proper forum for the discussion of issues pertaining and involving genome and/or chromatin structure and function (see _Topics of Discussion_). Primarily it should enable those researchers who work in genome/chromatin structure or related fields to communicate ideas and information, as well as, provide a chance for collaboration among national and international research groups. --------------------------------------------------------------------- Topics of Discussion include: 1. Genome/chromatin accessibility and recombination -recombination hotspots (mieotic and mitotic) -fragile sites -imprinting and recombination rates -ectopic gene targeting and chromatin structure 2. 3D-organization of the nucleus -chromosome territories -nucleoli -nuclear lamina (telomere localization) -tissue or cell cycle dependent positioning of chromosomes -RNA tracking 3. Effect of Superhelicity and DNA topology/structure(Triple strand, Z-DNA, cruciform, bent etc) on biological processes such as: -replication (ex. replication fork barriers, initiation sites) -transcription (ex. promoter function in relation to superhelicity) -recombination (ex. Gin and Hin invertases and superhelicity) 4. Histones and Nucleosomes and chromatin structure/function -H1 repression of transcription -Post translational modification of histones acetylation (H4, H3), phosphorylation (H1, H3) and ubiquitination (H2A, H2B) -Histone variants (ex. H2A.Z in mammals, H5 of chicken) 5. Models of genome structure (Loop Domain Model, Channel Model, MegaBase Giant Loop Model, etc.) 6. Classical chromosome elements and their relationship to gene function -centromeres (constitutive heterochromatin and gene silencing) -telomeres (associated silencing and involvement in position effect) 7. Evolution of the Genome -isochores and base-content (GC vs. AT) -formation of gene clusters and syntenic mapping -repetitive elements (satellites, telomeric and centromeric (alpha) repeats, lines and sines) 8. Biologically important mutants and knockouts that affect genome/chromatin structure -ex. SNF/SWI, TOPO mutants in yeast -RAD 51,52,54 knockout mice -AT, BLM, FA mouse models 9. Techniques for genome/chromatin analysis -cytogenetic techniques (g banding, r and q) -Fluorescent In situ analysis(FISH) -Confocal microscopy -Electron microscopy -Electron microscopy In situ analysis (EMISH) -psoralen, polyamine crosslinking -In vivo nucleosome foot printing -Dnase I/Micrococcal Nuclease sensitivity -VM26 Topoisomerase II site mapping 10. Chromatin/DNA binding proteins and their effects on chromatin structure and/or gene expression -Polycomb proteins -Rap1 (telomere silencing) -alpha2-MCM1 (repression of MAT locus) -CENP A/B/C (centromere structure/function) -XCAP-C/E, SMC1/2 (chromatin Condensation) -remodeling of chromatin by SWI/SNF proteins -H-NS in bacteria (role in nucleoid topology vs. gene function) 11. Nuclear Matrix (NM) and Nuclear Lamina (NL) -cell cycle regulation of formation of NM and NL -transcription and replications factors and the concept of a dynamic NM (ex. tissue and temporal specificity) -role of NM in signal transduction (mechanistic vs. chemical signals) 12. Matrix attatchent regions (MAR's), domain boundaries and locus control regions (LCR's) and their relationship to gene structure and function. -definition of transcription/replication domains -model systems ex. betaglobin (LCR) SCS/SCS' of the Drosophila Heat Shock Locus (HS87a7) 13. Phenomenon of Position Effect and Transvection -in drosophila (HP1, polycomb, heterochromatization) -in mammalian systems (silencing or variegated expression of transgenes) -in fungi 14. Epigenetic effects on gene function -imprinting -methylation -maintenance of early/late replication 15. Dosage compensation mechanisms and X chromosome inactivation -MSL proteins of Drosophila -XIC (Xist RNA) in mammals -CpG methylation 16. Chromatin structure and DNA replication -ORC1 protein of yeast -origins of replication (association with cruciform, bent DNA and MARS) 17. Specialized methods of nuclear packaging -bacterial nucleoid -packaging of DNA in spermatozoa 18. DNA repair and chromatin structure -TFIIH (transcription coupled repair) -p53 -BLM and AT genes -poly-ADP-polymerase (PARP) 19. Mechanisms of genomic instability -during oncogenesis -process of chromosomal aberration (stable and unstable) (ex. role of micronuclei in the process) -chromosomal aberration during aging and mechanisms of instability (ex. telomeres and telomerase) In addition this newsgroup provides: A forum for the exchange of information about future congresses and meetings in areas of molecular biology relating to genome structure/function. A forum for the exchange of information about textbooks, internet resources, visual materials, and computer programs. A source of quick help for last-minute troubleshooting, sources of materials, and practical advice; in areas such as -Specific common resources (ex. primers, antibodies, vectors) -Genome analysis techniques -Transgenic models and mutant cell lines Moderation Policy: Mass-posted commercial messages, chain letters, and similar postings not associated with or pertaining to the study of genome/chromatin structure and function will be deleted without comment. Inappropriate messages posted in good faith will be returned to the sender. Messages not strictly within the charter but likely to be of interest to many subscribers (e.g., messages dealing with transcription and replication factors) will be accepted. Subscribers are welcome from universities or any academic institutions, government agencies, medical institutions, and industrial or commercial organizations. Contributions within the functions outlined above are encouraged. Proposed discussion leaders: *Note: Area of expertise is written in brackets Graham Dellaire, e-mail: dellaire@odyssee.net (gdella@po-box.mcgill.ca) Institut du Cancer de Montreal Centre de Recherche L.C. Simard 1560 Sherbrooke Street East, Montreal, Quebec, Canada H2L 4M1 telephone:1 (514) 876 6936; fax: (514) 876 5476 *(Expertise:chromatin structure and recombination; Recombination Access Mapping (RAM); genome structure analysis) Ronald Hancock, e-mail: ronald.hancock@crhdq.ulaval.ca Professor Laval University Cancer Research Centre 1 rue de l'Arsenal Quebec, Canada G1R 2J6 telephone: 1 (418) 691-5281; fax: 1 (418) 691-5439 *(Expertise:genome organisation in vivo; models of genome structure; topoisomerases; VM26 topoisomerase II site mapping) Eric Milot, e-mail: milot@ch1.fgg.eur.nl Faculty of Medicine Dept. of Cell Biology and Genetics P.O. Box 1738 3000 DR Rotterdam The Netherlands telephone: 3110 4087164; fax: 3110 4360225 *(Expertise:Chromatin context and transcription; Polycomb protein; position effect variegation; chromosome and nuclear organisation; dosage compensation) **Tentative Discussion leader** Peter Cook, e-mail: peter.cook@pathology.oxford.ac.uk Professor of Cell Biology, The Sir William Dunn School of Pathology, South Parks Road, Oxford, OX1 3RE, UK. Telephone (direct line) : +44/0 1865 275528 Telephone (switchboard) : +44/0 1865 275500 Fax : +44/0 1865 275501 http://www.molbiol.ox.ac.uk/www/users/counsell/cook.htm (Expertise:Chromatin/Genome Structure and Function in regard to transcription and replication) ---------------------------------------------------------------------- Voting is now open on the proposal for GENSTRUCTURE/bionet.genome.gene-structure and will run through 24:00 hrs Pacific Time on 12 December 1996. Please send your vote to either of the following addresses: Address Location Network ------- -------- ------- biovote@daresbury.ac.uk U.K. JANET biovote@net.bio.net U.S.A. Internet/BITNET PLEASE BE SURE TO FOLLOW THE FORMAT BELOW - WE OFTEN RUN MORE THAN ONE VOTE AT A TIME SO A SIMPLE "YES" OR "NO" MESSAGE WITHOUT THE NEWSGROUP NAME MAY BE AMBIGUOUS. Your vote should contain a single line: YES on GENES if you favor allowing the creation of this newsgroup or NO on GENES if you think that this proposal will adversely affect the BIOSCI/bionet system. While not intended to be an exhaustive list of possible concerns (more specific concerns may have been raised during the discussion period on BIOFORUM/bionet.general and interested readers are referred to these), some general reasons for voting NO might be if you are concerned about newsgroup proliferation and/or believe that the proposed group will not be utilized, or if you think that the proposed newsgroup would substantially duplicate or overlap with the function of existing newsgroups. If you are simply not interested in participating in the newsgroup above, please don't cast a NO vote, but instead just don't vote at all. The newsgroup proposal must receive at least 80 YES votes to pass and the number of YES votes must be greater than the number of NO votes by at least 40. Discussion of the newsgroup proposal is now closed and we strongly discourage posting any messages in other forums about the fact that a CALL FOR VOTES has been issued. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-recombination@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.recombination Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 18 Nov 1996 02:00:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611181000.CAA14391@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-recombination@net.bio.net Wed Nov 27 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: "Johan van Ooijen (+31) 317 477 319" Newsgroups: bionet.molbio.recombination Subject: JoinMap & MapQTL software announcement Date: 28 Nov 1996 14:27:46 -0000 Lines: 32 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <57k7h2$bgh@mserv1.dl.ac.uk> Autoforwarded: false Ua-Content-Id: 11ABE3DC1100 Hop-Count: 4 Importance: normal Disclose-Recipients: prohibited Original-To: gen-link@dl.ac.uk, bio-software@dl.ac.uk, pop-bio@dl.ac.uk, "/R=POST/R=AM_CPRO/U=biochrom@dl.ac.uk@IN@POST/"@gate.agro.nl, arab-gen@dl.ac.uk, recom@dl.ac.uk You can now read about the genetic mapping software packages JoinMap and MapQTL on the web site of CPRO-DLO: http://www.bib.wau.nl/cpro/mapping/ Information is presented on the things you can do with these software packages. You can also view the manuals to study the details, and you can view the lists of frequently asked questions. You can read about the computer platforms for which the software is available, how the licensing works, what it costs, and how to order. JoinMap is software for the calculation of genetic linkage maps. It handles data from all kinds of full-sib families (BC, F2, RILs, [doubled] haploids, cross-pollinator-progeny), and can combine data from several sources into an integrated map. Besides the modules for map calculation, the package has some diagnostical modules. MapQTL is software for the mapping of quantitative trait loci (QTLs). It handles data from single full-sib families of all kinds (BC, F2, RILs, [doubled] haploids, cross-pollinator-progeny). Used techniques: interval mapping, multiple QTL models with cofactors (MQM mapping), nonparametric Kruskal-Wallis analysis per marker. Johan van Ooijen email: J.W.vanOoijen@CPRO.DLO.NL or mapping@CPRO.DLO.NL fax: +31 317 418 094 mail: CPRO-DLO, POBox 16, 6700 AA Wageningen, the Netherlands DLO Centre for Plant Breeding and Reproduction Research (CPRO-DLO) WWW: http://www.bib.wau.nl/cpro/