From owner-recombination@net.bio.net Sat Feb 01 22:00:00 1997 Path: biosci!agate!ihnp4.ucsd.edu!swrinde!howland.erols.net!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!uunet!in1.uu.net!38.8.16.2!psinntp!cmcl2.nyu.edu!news.nyu.edu!not-for-mail From: sms1894@is.nyu.edu (Sanjay Shirke) Newsgroups: bionet.molbio.recombination Subject: Re: recombinant embryo? Date: 2 Feb 1997 21:57:49 GMT Organization: New York University Lines: 20 Message-ID: <5d32kt$24c$1@news.nyu.edu> References: <5cqoql$a3h@chaos.dac.neu.edu> NNTP-Posting-Host: is.nyu.edu X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Joshua Mularella (jmularel@lynx.dac.neu.edu) wrote: : I'm doing a report on jurassic park and was wondering if you think it : would be possible to somehow place dino dna in a bird egg and sperm, : combine the two, and attach the zygote in the uterus of the bird to let : it grow. What do you think? : : Joshua Mularella Won't work. For starters, how's a dino embryo gonna fit in a bird eggshell, huh? &c. ------------------------------ s a n j a y m . s h i r k e From owner-recombination@net.bio.net Mon Feb 17 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.recombination Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 18 Feb 1997 02:00:11 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199702181000.CAA02695@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-recombination@net.bio.net Thu Feb 20 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!newspump.sol.net!howland.erols.net!vixen.cso.uiuc.edu!prairienet.org!clittle From: clittle@prairienet.org (Carol Little) Newsgroups: bionet.molbio.yeast,bionet.molbio.recombination Subject: uh... Date: 21 Feb 1997 09:24:12 GMT Organization: University of Illinois at Urbana Lines: 2 Message-ID: <5ejpjs$bps@vixen.cso.uiuc.edu> Reply-To: clittle@prairienet.org NNTP-Posting-Host: bluestem.prairienet.org Xref: biosci bionet.molbio.yeast:6646 bionet.molbio.recombination:357 testing... From owner-recombination@net.bio.net Wed Feb 26 22:00:00 1997 Path: biosci!daresbury!bioftp.unibas.ch!news.vub.ac.be!news.belnet.be!news.rediris.es!news.uoregon.edu!newsfeed.orst.edu!wilbur.sequent.com!uunet!in3.uu.net!198.151.175.33!news.xensei.com!news From: chi@healthtech.com (Cambridge Healthtech Institute) Newsgroups: bionet.molbio.recombination Subject: Cambridge Healthtech Institute’s MOLECULAR EVOLUTION Date: Thu, 27 Feb 1997 18:15:10 GMT Organization: Cambridge Healthtech Institute Lines: 129 Message-ID: <5f4iq5$qqc@xensei3.xensei.com> Reply-To: chi@healthtech.com NNTP-Posting-Host: chi.xensei.com X-Newsreader: Forte Free Agent 1.0.82 Cambridge Healthtech Institute’s MOLECULAR EVOLUTION April 24-25, 1997 Copley Plaza, Boston, Massachusetts Co-sponsored by: Canji, Inc., Pharmacia Biotech, and PharmaGenics A large number of biomedical applications involve the search for compounds with improved activity or performance. The application of molecular evolution is proving to be extremely well suited for this purpose, particular for identification of nucleic acids or peptides. Genetics makes it possible to employ sequential rounds of screening and selection, followed by further mutation, to allow for the generation of compounds adapted for a desired target or under specific conditions. Phage display and other related techniques have been used extensively for this purpose. Examples of the development of antibodies, mutated proteins, and a range of research, selection, and therapeutic applications are covered in detail. Learn how leaders in this field are taking advantage of the power of this technology and how you might benefit from making greater use of it by attending this timely event. NUCLEIC ACID EVOLUTION In Vivo and In Vitro Applications of Nucleic Acid Ligands Dr. Daniel W. Drolet, NeXstar Pharmaceuticals (confirmed) Mirror-Image RNA Ligands Dr. Jens P. Furste, Freie Universitst Berlin (confirmed) Aptamer Evolution: Prospects for Diagnostics and Therapeutics Dr. Andrew D. Ellington, Indiana University (confirmed) Ribozymes from Random Sequences: Dr. David Bartel, Whitehead Institute and Massachusetts Institute of Technology (confirmed) ENGINEERING PROTEINS AND ANTIBODIES Exploiting the Diversity of Large Human Antibody Libraries Dr. John McCafferty, Cambridge Antibody Technology Ltd. (confirmed) Molecular Evolution of Proteins, Pathways and Viruses by DNA Shuffling Dr. Willem Stemmer, Maxygen, Inc. (confirmed) In Vitro Selection of Peptides Acting on NMDA Glutamate Receptors Dr. Min Li, John Hopkins School of Medicine (confirmed) Synthesis Based Molecular Design of Antibodies and Proteins: Dr. William D. Huse, Ixsys, Inc. (confirmed) Dr. Manuel Baca, Genentech, Inc. (confirmed) RESEARCH AND SELECTION USING PHAGE DISLAY Cloning Novel Genes with Phage-Displayed Peptide Ligands Dr. Brian Kay, University of North Carolina at Chapel Hill (confirmed) Adenovirus Expression Systems in Conjunction with Autofluorescent Proteins: Potential Use in the Screening of Peptide Libraries Dr. Luc Peloquin, Quantum Biotechnologies Inc. (confirmed) Functional Epitope Mapping by Negative Selection of Phage-Displayed Randomized Protein Libraries Dr. Laurent Jespers, Flanders Interuniversity Institute for Biotechnology (confirmed) Automated Affinity Based Screening Using Serial Column Chromatography Interfaced with Mass Spectrometry Dr. Satish Jindal, ChemGenics Pharmaceuticals (confirmed) Use of Combinatorially Generated Phage Displayed Ligands to Develop Engineered Microprotein Ligands for Large Scale Biotherapeutic Purification Dr. John Maclennan, Dyax Corporation (confirmed) Identification of Optimal Ligands for Protein Domains Using Oriented Peptide Libraries Dr. Zhou (Sonny) Songyang, Harvard Medical School (confirmed) THERAPEUTIC DEVELOPMENTS USING PHAGE DISPLAY Identification of Amino Acids Critical for the Activity of a Peptide Mimetic of Erythropoietin and Discription of a Minimal Functional Epitope Dr. Dana Johnson, R.W. Johnson Pharmaceutical Research Institute (confirmed) Combinatorial Methods and Directed Evolution Applied to Staphylococcal a-hemolysin, a Pore-Forming Protein Dr. Hagan Bayley, Worcester Foundation for Biomedical Research (confirmed) Human Protein Display: A New System for Discovering Drug Binding Targets Dr. Allan Peng, GeneMax, Inc. (confirmed) Novel Approaches for Targeting Tumors and Inhibiting Metastasis Dr. Renata Pasqualini, La Jolla Cancer Research Center, The Burnham Institute (confirmed) Sampling Peptide Space for Cell-Targeting Peptides Dr. Stephen A. Johnston, University of Texas, Southwestern Medical Center, (confirmed) Cloning Using Phage Display Dr. Bob Shopes, Tera Biotechnology Corporation (confirmed) Establishing Site Directed Assays Using Phage Displayed Libraries of Peptides and Antibodies Dr. Arthur J. Blume, DGI BioTechnologies, L.L.C. (confirmed) From the rapid progess of combinatorial chemistry and genetics an interest has been generated in the selection of random peptides from target proteins and in investigating ligand-receptor interactions. Some challenges that will be examined are the screening of combinatorial libraries for selecting high-affinity ligands against target molecules, following the sequential cycles of random mutagenesis and screening to direct the evolution of enzymes, and developing new techniques such as phage display for optimum peptide /receptor binding. The expectation that from the selection and use of phage display, proteins for therapeutics and drug discovery may be developed. Advance registration March 7, 1997 poster deadline March 21, 1997 For registration and hotel information, please contact: Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 USA Phone: 617-630-1300 Fax: 617-630-1325 e-mail: chi@healthtech.com http://www.healthtech.com/conferences/ ______________________________ Cambridge Healthtech Institute 1037 Chestnut Street Newton Upper Falls, MA 02164 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ tel: 617.630.1300 fax: 617.630.1325 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ inquiries@healthtech.com World Wide Web http://www.healthtech.com/conferences From owner-recombination@net.bio.net Thu Feb 27 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.erols.net!newsfeed.internetmci.com!news.dtinet.or.jp!ppp40!aisoai From: aisoai@agc.co.jp (ISOAI Atsushi) Newsgroups: bionet.molbio.recombination Subject: WWW for the Molecular Biologists Date: Sat, 01 Mar 1997 01:43:26 +0900 Organization: Research Center, Asahi Glass Co.LTD. Lines: 15 Message-ID: NNTP-Posting-Host: ppp40.yokohama2.dtinet.or.jp Mime-Version: 1.0 Content-Type: text/plain; charset=iso-2022-jp X-Newsreader: NewsWatcher-J 2.0J16 Hi all netters; I have uploaded Web site for the biochemists and molecular biologists. In my site, you can find very useful database, www, ftp, gopher and other useful sites on the net. Moreover, my sites present many sites which offer you software or tools for the research. Please visit and enjoy! [Sites for the Molecular Biology - LINKS] URL: http://www.yk.rim.or.jp/~aisoai/index.html I look forward to hearing your suggestions and comments from you. ================================================================= Atsushi Isoai, Ph.D. Senior Staff Researcher, Research Center, Asahi Glass Co.LTD. URL: http://www.yk.rim.or.jp/~aisoai/index.html =================================================================