From owner-recombination@net.bio.net Thu May 01 23:00:00 1997 Path: biosci!VAXA.CIS.UWOSH.EDU!lansman From: lansman@VAXA.CIS.UWOSH.EDU (Bob Lansman) Newsgroups: bionet.molbio.recombination Subject: Chi-like sites in eukaryotes Date: 2 May 1997 06:51:40 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 8 Sender: daemon@net.bio.net Distribution: world Message-ID: <2.2.32.19970502135241.0068f9e0@vaxa.cis.uwosh.edu> NNTP-Posting-Host: net.bio.net A paper in the April, 1997, Genetics (Dabert and Smith, 145:877-899), demonstrates that Chi sites flanking linear fragments stimulate gene replacement in bacteria in a RecBCD-dependent pathway. It would be of extreme interest to be able to achieve high rates of gene replacement in the eukaryotes. Does anyone know of any evidence for the existence of sequences with Chi-like activity in a eukaryote? Thanks, Bob From owner-recombination@net.bio.net Fri May 02 23:00:00 1997 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: Re:Chi-like sites in eukaryotes Date: 3 May 1997 06:05:10 -0700 Organization: McGill Div. of Experimental Medicine Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: <336B377C.7B42@odyssee.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net Bob, In eukaryotes you can use SceI enzyme site (18 bp cutter from yeast which doesn't cut in murine or human genome) in the sequence you want to targer and you can increase homologous targeting 100 fold (Maria Jasin's work see:Mol. Cell. Biol. 14(12), 8096-8106 (1994) and review in Trends Genet. 12(6), 224-228 (1996) ) and illegitimate 1000 fold (Recent paper from Wilson's Group (Mol. Cell. Biol. 17(1), 267-277 (1997) ). As well you have the site specific eukaryote systems like Cre Lox (from a bacteriophage) and Flp recombinase from yeast (look for the original work on this enzyme by Dr. P. Sadowski at U of Toronto). For a natural site specific event in mammals look at all VDJ recombination work where you have haptamer and nonamer signal sequences for site specific recombination. Lastly there are quite a few papers on the instability of microsatellites (di and trinucleotide repeats) and fragile sites (like fragile X) in which you can preferentially integrate at a high frequency (relative to "random" illegitimate integration) your transgene. The common thread seems to be the ability to make a double strand break (DSB) or have a chromatin structure that is more accessible to the formation of a DSB. Cheers, Graham Dellaire From owner-recombination@net.bio.net Sat May 03 23:00:00 1997 Path: biosci!GPU.SRV.UALBERTA.CA!hastings From: hastings@GPU.SRV.UALBERTA.CA (P Hastings) Newsgroups: bionet.molbio.recombination Subject: Re: Chi-like sites in eukaryotes Date: 4 May 1997 08:04:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <2.2.32.19970502135241.0068f9e0@vaxa.cis.uwosh.edu> NNTP-Posting-Host: net.bio.net On 2 May 1997, Bob Lansman wrote: > A paper in the April, 1997, Genetics (Dabert and Smith, > 145:877-899), demonstrates that Chi sites flanking linear fragments > stimulate gene replacement in bacteria in a RecBCD-dependent pathway. It > would be of extreme interest to be able to achieve high rates of gene > replacement in the eukaryotes. Does anyone know of any evidence for the > existence of sequences with Chi-like activity in a eukaryote? > Thanks, Bob > > > Bob Grahm answered part of your question. I can have a go at the other part. There is almost no evidence to cause us to expect chi-like sites to operate in eukariotes. The only evidence I have ever heard of is that there is a concentration of chi-like sites in the switching regions of immunoglobulin genes. These are, of course, hotspots for homologous recombination. But beyond that titillating datum, I believe that we should not expect chi to operate in other systems. I think of it as a parochial aspect of the coli system. First, only one other bacterium (Iforget which) has been reported to have such signals, and they do not look like chi. second, the need for chi is caused by the RecD product. Targetted integration is readily acheived in coli by mutating out recD. RecD turns the RecBC helicase (recombinase) into a double stranded exonuclease. Chi changes it back to a recombinase. Homologues to RecC can be found in other organisms, but I have not heard of a RecD homologue. So I go arround expecting that most other organisms will be like recD minus coli- they will have helicases and single strand nucleases operating at double-strand breaks to make them recombinagenic and will not need a signal like chi. Oops! I nearly forgot- there was a report of a preferred sequence for stopping the action of the neurospora endo-exo nuclease of Murray Fraser. Terry, you had better report on that part of it for us. Phil. From owner-recombination@net.bio.net Sat May 03 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!news-out.communique.net!communique!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!feed1.news.erols.com!news.idt.net!usenet.logical.net!news.dal.ca!newsflash.concordia.ca!news.mcgill.ca!news From: Terry Chow Newsgroups: bionet.molbio.recombination Subject: Re: Chi-like sites in eukaryotes Date: Sun, 04 May 1997 13:06:57 -0400 Organization: McGill University Computing Centre Lines: 47 Message-ID: <336CC231.7FD8@musica.mcgill.ca> References: <2.2.32.19970502135241.0068f9e0@vaxa.cis.uwosh.edu> NNTP-Posting-Host: 198.168.147.137 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win95; I) P Hastings wrote: > > On 2 May 1997, Bob Lansman wrote: > > > A paper in the April, 1997, Genetics (Dabert and Smith, > > 145:877-899), demonstrates that Chi sites flanking linear fragments > > stimulate gene replacement in bacteria in a RecBCD-dependent pathway. It > > would be of extreme interest to be able to achieve high rates of gene > > replacement in the eukaryotes. Does anyone know of any evidence for the > > existence of sequences with Chi-like activity in a eukaryote? > > Thanks, Bob > > > > > > > Bob Grahm answered part of your question. I can have a go at the > other part. There is almost no evidence to cause us to expect chi-like > sites to operate in eukariotes. The only evidence I have ever heard of is > that there is a concentration of chi-like sites in the switching regions > of immunoglobulin genes. These are, of course, hotspots for homologous > recombination. But beyond that titillating datum, I believe that we > should not expect chi to operate in other systems. I think of it as a > parochial aspect of the coli system. First, only one other bacterium > (Iforget which) has been reported to have such signals, and they do not > look like chi. second, the need for chi is caused by the RecD product. > Targetted integration is readily acheived in coli by mutating out recD. > RecD turns the RecBC helicase (recombinase) into a double stranded > exonuclease. Chi changes it back to a recombinase. Homologues to RecC can > be found in other organisms, but I have not heard of a RecD homologue. So > I go arround expecting that most other organisms will be like recD minus > coli- they will have helicases and single strand nucleases operating at > double-strand breaks to make them recombinagenic and will not need a > signal like chi. > Oops! I nearly forgot- there was a report of a preferred sequence > for stopping the action of the neurospora endo-exo nuclease of Murray > Fraser. Terry, you had better report on that part of it for us. > Phil. Thanks Phil, Murray Fraser have analyzed the action of the putative recombination nuclease, the endo-exonuclease, with pBR322 as substrat. He found there is a consensus sequence that the enzyme recognized at the nick site. This sequence is AGCACT. This sequence is obtained by sequence analysis of the many nick sites, therefore, it should be consider as consensus rather than absolute sequence as the case with Chi site. Terry Chow From owner-recombination@net.bio.net Sun May 04 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!rill.news.pipex.net!pipex!oleane!jussieu.fr!ravel.ijm.jussieu.fr!user From: bregeon@ijm.jussieu.fr (Bregeon Damien) Newsgroups: bionet.molbio.recombination,bionet.microbiology,sci.bio.microbiology Subject: plasmid Date: Mon, 05 May 1997 19:03:52 +0100 Organization: IJM Lines: 10 Message-ID: NNTP-Posting-Host: ravel.ijm.jussieu.fr Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit X-Newsreader: Value-Added NewsWatcher 2.0b24.0+ Xref: biosci bionet.molbio.recombination:439 bionet.microbiology:9856 sci.bio.microbiology:5908 We are looking for a E. coli plasmid that carries lacIq and LacZ. Preferably with a kanamycin resistance. thanks in advance for your help. -- BREGEON Damien Institut Jacques MONOD 2, Place Jussieu 75251 Paris cedex05 e-mail : bregeon@ijm.jussieu.fr From owner-recombination@net.bio.net Mon May 05 23:00:00 1997 Newsgroups: bionet.molbio.recombination Path: biosci!rutgers.rutgers.edu!csn!nntp-xfer-1.csn.net!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!news.maxwell.syr.edu!EU.net!uknet!usenet1.news.uk.psi.net!uknet!uknet!bcc.ac.uk!news From: Mr Paul Wells Subject: Practical introduction to genetic engineering course Message-ID: <1997May6.122339.38789@ucl.ac.uk> Date: Tue, 6 May 1997 12:23:39 GMT Organization: University College London Lines: 35 A one week practical course designed to teach the basic techniques in genetic engineering will be held from 16th-20th June 1997 at the Dept. of Biochemistry and Molecular Biology University College London. A "hands on" approach will enable scientists from higher education and industry, clinicians and research workers who have had no practical experience of recombinant DNA technology to set up such techniques in their own laboratories. Techniques to be covered will include, Southern hybridisation DNA sequencing, PCR, and analysis of cloned DNA using enzyme restriction mapping. Supplemented with some lectures. Course fee 900 pounds sterling (750 for full time postgraduate students) exclusive of accommodation. Bed and breakfast accommodation for the period of the course can be provided in a local hall of residence for 102 pounds sterling from Sunday 15th June until Saturday 21st June. For further information visit our web site "http://www.biochem.ucl.ac.uk/~wells/pige97.htm Write for application forms to: Dr. John Ward Department of Biochemistry & Molecular Biology University College London Gower Street London WC1E 6BT Fax: 0171 380 7193 Or e-mail either:- j.ward@biochem.ucl.ac.uk p.wells@ucl.ac.uk From owner-recombination@net.bio.net Mon May 12 23:00:00 1997 Path: biosci!ERE.UMontreal.CA!houleb From: houleb@ERE.UMontreal.CA (houle) Newsgroups: bionet.molbio.recombination Subject: FASEB RECOMBINATION CONFERENCE (AUG. 2-7,1997) Date: 13 May 1997 09:41:06 -0700 Organization: Institut du Cancer de Montreal Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <3378B5A4.444F@ere.umontreal.ca> Reply-To: houleb@ERE.UMontreal.CA NNTP-Posting-Host: net.bio.net FASEB SUMMER RESEARCH CONFERENCE 1997 Snowmass Village Colorado, USA See: http//www.faseb.org/meetings/src/snowmass.htm GENETIC RECOMBINATION AND CHROMOSOME REARRANGEMENTS August 2-7 James E. Haber, Chair Stephen Kowalczykowski, Co-chair 3 August. DNA Replication in Recombination and Repeat Instability. T.D. Petes, S. Warren, T. Kogoma, S. Lovett. Site-specific Recombination. T. Baker, K. Mizuuchi, N. Craig, D. Sherratt. 4 August. Mechanisms of Recombination. R. Rothstein, R. Kanaar, P. Sung, L. Symington. Bacterial Recombination Mechanisms. J. Roth, S. West. M. Belfort, C. Radding. 5 August. Immunoglobulin Gene Rearrangements. M. Oettinger, M. Gellert, M. Schlissel, D. Roth. Protein Structures in Recombination. E. Egelman, D. Wigley, D. Rice, T. Shibata. 6 August. Mammalian Recombination and Gene Targeting. M. Liskay, J. Lupski, D. Weaver, E. Kmiec. Homeologous and Ectopic Recombination. S. Jinks-Robertson, M. Radman, R. Borts, R.D. Kolodner. 7 August Meiotic Recombination. J. Kohli, S. Roeder, N. Kleckner, M. Lichten. Additional speakers will be selected from submitted abstracts. Deadline for receipt of abstracts is June 1. Cheers, Graham Dellaire From owner-recombination@net.bio.net Thu May 15 23:00:00 1997 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: NEW ON EMJL JOB LISTINGS (3 Career Scientist Positions!!!, Ottawa, Canada) Date: 15 May 1997 18:07:39 -0700 Organization: McGill Div. of Experimental Medicine Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: <337BB264.32EF@odyssee.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net New on the EMJL Job Listings http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html ================================================================= Three vacant positions at the Ottawa Regional Cancer Center 1.A molecular geneticist (perhaps working in simple eukaryotes (i.e. yeast)) interested in functions such as DNA repair, recombination, chromatin structure, apoptosis, etc. 2.A cellular or animal physiologist interested in how cells, tissues or organs respond to insults such as those induced by radiation, and 3.A radiation oncologist with training in research interested in translating research findings from simple systems into clinical research. These positions that are available are those of Career Scientists or Clinical Scientists of the Ontario Cancer Treatment and Research Foundation. These are permanent research poisions within the Ottawa Regional Cancer Center and involve affiliation within the Faculty of Medicine at the University of Ottawa. We have abundant laboratory space available for this expanded group along with start-up funding. To find out more about how to apply to this job and others on EMJL please see the EMJL Home Page at: http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html (for this job look under Positions/Canada/Ontario) From owner-recombination@net.bio.net Sat May 17 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.recombination Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 18 May 1997 02:00:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199705180900.CAA08945@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-recombination@net.bio.net Thu May 22 23:00:00 1997 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET ("Graham Dellaire") Newsgroups: bionet.molbio.recombination Subject: NEW on EMJL (JOBS in BIOMEDICINE/MOLBIO) Date: 22 May 1997 21:23:20 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <97May23.002355-0400_edt.586110-221+6407@skywalker.microtec.net> NNTP-Posting-Host: net.bio.net NEW ON Experimental Medicine Job Listings (EMJL) Please see: http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html for information on the following jobs. 1.Research Technician BioSignal Inc. (Montreal, Quebec, Canada) A Research Technician position with BioSignal. BioSignal is a leader in the development and commercial supply of cloned targets as reagents for drug screening. The company is seeking highly motivated and team orientated technician to work in its R&D department. Salary will commensurate with experience. Position availbale immediately until filled If you wish to apply to this job see: http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html#MA 2. Research Assistant, MethylGene Inc. (Montreal, Quebec, Canada) Project description: A Research Assistant position is available with Methylgene Inc. MethylGene Inc., is a growing Montreal-based biotech company located in the newly constructed CITECH biotech park in Saint-laurent The Company is dedicated to the development of an exciting new class of compounds for the treatment of cancer. They are currently seeking highly skilled research technicians with experience in the following areas: isolation of high quality RNA and Northern blot protocols for sensitive mRNA's RNase protection and S1 nuclease assays Maintenance of stable cell lines in culture In vitro wnzymatic assays Western Blot protocols Salary will commensurate with experience. Position availbale immediately until filled If you wish to apply to this job see: http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html#MA From owner-recombination@net.bio.net Mon May 26 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.erols.net!newsfeed.internetmci.com!netnews.nwnet.net!news-hub.interserv.net!news.sprynet.com!not-for-mail From: Newsgroups: bionet.molbio.proteins,bionet.molbio.recombination,bionet.molbio.peptides,bionet.general Subject: Want ABI 394 Date: 27 May 1997 17:49:45 GMT Organization: Sprynet News Service Lines: 6 Message-ID: <5mf6rp$2qb$1@juliana.sprynet.com> NNTP-Posting-Host: ad31-202.compuserve.com Content-Type: text/plain Content-length: 101 X-Newsreader: AIR Mosaic (16-bit) version 4.16.16.05 Xref: biosci bionet.molbio.proteins:10837 bionet.molbio.recombination:467 bionet.general:26970 Wanted: ABI 394; will pay $10,000. Mike Sherrell Grizzly Analytical 707 887 2919 fax 707 887 9834 From owner-recombination@net.bio.net Mon May 26 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.erols.net!newsfeed.internetmci.com!netnews.nwnet.net!news-hub.interserv.net!news.sprynet.com!not-for-mail From: Newsgroups: bionet.molbio.proteins,bionet.molbio.recombination,bionet.molbio.peptides,bionet.general Subject: Want ABI 394 Date: 27 May 1997 17:44:38 GMT Organization: Sprynet News Service Lines: 6 Message-ID: <5mf6i6$rj$1@juliana.sprynet.com> NNTP-Posting-Host: ad31-202.compuserve.com Content-Type: text/plain Content-length: 101 X-Newsreader: AIR Mosaic (16-bit) version 4.16.16.05 Xref: biosci bionet.molbio.proteins:10835 bionet.molbio.recombination:465 bionet.general:26968 Wanted: ABI 394; will pay $10,000. Mike Sherrell Grizzly Analytical 707 887 2919 fax 707 887 9834 From owner-recombination@net.bio.net Mon May 26 23:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!hammer.uoregon.edu!ais.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!netnews.nwnet.net!news-hub.interserv.net!news.sprynet.com!not-for-mail From: Newsgroups: bionet.molbio.proteins,bionet.molbio.recombination,bionet.molbio.peptides,bionet.general Subject: Want ABI 394 Date: 27 May 1997 17:43:25 GMT Organization: Sprynet News Service Lines: 6 Message-ID: <5mf6ft$9l$1@juliana.sprynet.com> NNTP-Posting-Host: ad31-202.compuserve.com Content-Type: text/plain Content-length: 101 X-Newsreader: AIR Mosaic (16-bit) version 4.16.16.05 Xref: biosci bionet.molbio.proteins:10836 bionet.molbio.recombination:466 bionet.general:26969 Wanted: ABI 394; will pay $10,000. Mike Sherrell Grizzly Analytical 707 887 2919 fax 707 887 9834 From owner-recombination@net.bio.net Tue May 27 23:00:00 1997 Path: biosci!ODYSSEE.NET!dellaire From: dellaire@ODYSSEE.NET (Graham Dellaire) Newsgroups: bionet.molbio.recombination Subject: NEW JOBS!!! FIND THEM FRESH ON EMJL!!!(GROUP LEADER AT ICRF!) Date: 28 May 1997 15:36:23 -0700 Organization: McGill Div. of Experimental Medicine Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: <338CB281.E73@odyssee.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net NEW ON EMJL!! Job Listings in Biomedical Science!!! ================================================== #1) 1 Ph.D Postion to characterize a novel fish retrovirus under the supervision of Dr. D. Martineau (University of Montreal)DEADLINE: June 30th, 1997 see http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html (Choose Positions/Canada/Montreal/) #2) 4 Group Leader Positions at the Imperial Cancer Research Fund (London, England) Deadline JUNE 20, 1997 see http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html (Choose Positions/Outside of Canada/Europe/Great Britain) =================================================== Please visit EMJL Home Page http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html For further information on how to apply to these job listings. DO NOT REPLY DIRECTLY TO THIS POST. ALL DIRECT REPLIES WILL BE DELETED WITHOUT COMMENT From owner-recombination@net.bio.net Wed May 28 23:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!harbinger.cc.monash.edu.au!not-for-mail From: Active Tools Inc Newsgroups: bionet.molbio.recombination,bionet.xtallography,comp.graphics.apps.avs Subject: Use your networked computers for large scale simulations Date: Fri, 30 May 1997 09:39:36 +1000 Organization: Active Tools Lines: 72 Message-ID: <338E13B8.5CDF@activetools.com> Reply-To: info@activetools.com NNTP-Posting-Host: davida.dgs.monash.edu.au Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01Gold (Win95; I) Xref: biosci bionet.molbio.recombination:473 bionet.xtallography:3497 FOR IMMEDIATE RELEASE Contact: Sergij Foski Active Tools, Inc. Phone: (415) 882-7062 Fax: (415) 680-2369 email: sergij.foski@activetools.com Active Tools Announces Clustor(tm) 1.1 for Exploiting the Combined Power of Networked Computers San Francisco, CA - May 12, 1997 -- Active Tools, Inc. today announced the release of Clustor 1.1, a software tool for distributing and managing computationally intensive tasks. "Clustor delivers computing power to users with ever increasing needs for more computing cycles." said Rok Sosic, President and CEO of Active Tools, Inc. "With Clustor, they can routinely combine the power of networked computers." Clustor greatly simplifies and speeds up parametric executions - running the same application numerous times with different input parameters. It generates jobs, speeds up the task by distributing the jobs over a network and collects the results. Jobs can be distributed over a local area network or over Internet. Version 1.1 of Clustor provides new features for load monitoring and resource sharing. Clustor is targeted at users, such as engineers, scientists and researchers. It provides an intuitive graphical user interface for all phases of executing jobs on a network of computers: from task preparation, job generation to job execution. Now, the combined power of networked computers can be exploited by users with no knowledge of programming parallel or distributed applications. Clustor is already being used in fields, such as VLSI circuit design, electronic design automation, bioinformatics, operations research, computer graphics, ecological and environmental modeling, laser and particle physics. According to Dr. Andrej Sali, Assistant Professor at Laboratory of Molecular Biophysics, Rockefeller University in New York "We found Clustor extremely helpful in squeezing more CPU time from our workstations, resulting in a highly increased job turnaround time and productivity." Clustor version 1.1 is currently available for major Unix environments such as Digital Unix, Hewlett Packard HP-UX, IBM AIX, Silicon Graphics Irix, Sun Solaris and Linux. A version of Clustor for Windows NT environment will be released later this year. Clustor 1.1 for Unix is priced at $695. Additional computational nodes are $149. Clustor 1.1 for Linux is priced at $495 and $99, respectively. Academic discount of 40% is available to academic institutions for non-commercial use. Clustor can be purchased directly from Active Tools. Copies of Clustor and free 30-day evaluation licenses are available at http://www.activetools.com/. Active Tools, founded in 1995, is a developer of software tools for high performance computing. The founders have been developing software tools for more than 15 years in both industry and academia. Active Tools is a privately held company with offices in United States and Australia. Clustor and Clustor Node are trademarks of Active Tools, Inc. All other company and product names mentioned may be trademarks or registered trademarks of the respective companies with which they are associated.