From owner-recombination@net.bio.net Sun Feb 01 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: NEW HOT PAPERS for January/February Part 1 Date: 1 Feb 1998 20:03:12 -0800 Organization: McGill Div. of Experimental Medicine Lines: 93 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6b3gi0$5dn@net.bio.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net HOT PAPERS for January/February 1998 in Recombination Part 1 (compiled by G. Dellaire) Copenhaver GP, et al. Assaying genome-wide recombination and centromere functions with arabidopsis tetrads. Proc Natl Acad Sci U S A. 1998 Jan 6; 95(1): 247-252. Nicolas A. Relationship between transcription and initiation of meiotic recombination: toward chromatin accessibility. Proc Natl Acad Sci U S A. 1998 Jan 6; 95(1): 87-89. Yang X, et al. Torsional control of double-stranded DNA branch migration. Biopolymers. 1998; 45(1): 69-83. Dale A. Ramsden and Martin Gellert Ku protein stimulates DNA end joining by mammalian DNA ligases: a direct role for Ku in repair of DNA double-strand breaks EMBO J. 1998 17: 609-614 Eiichiro Sonoda, Masao S. Sasaki, Jean-Marie Buerstedde, Olga Bezzubova, Akira Shinohara, Hideyuki Ogawa, Minoru Takata, Yuko Yamaguchi-Iwai, and Shunichi Takeda Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death EMBO J. 1998 17: 598-608 Nagawa F, et al. Footprint analysis of the RAG protein recombination signal sequence complex for V(D)J type recombination. Mol Cell Biol. 1998 Jan; 18(1): 655-663. Schwartz A, et al. Reconstructing hominid Y evolution: X-homologous block, created by X-Y transposition, was disrupted by Yp inversion through LINE-LINE recombination. Hum Mol Genet. 1998 Jan; 7(1): 1-11. hierry P. Naas, Ralph J. DeBerardinis, John V. Moran, Eric M. Ostertag, Stephen F. Kingsmore, Michael F.Seldin, Yoshihide Hayashizaki, Sandra L. Martin, and Haig H. Kazazian Jr An actively retrotransposing, novel subfamily of mouse L1 elements EMBO J. 1998 17: 590-597. E. Pipiras, A. Coquelle, A. Bieth, and M. Debatisse Interstitial deletions and intrachromosomal amplification initiated from a double-strand break targeted to a mammalian chromosome EMBO J. 1998 17: 325-333. Ola Hammarsten and Gilbert Chu DNA-dependent protein kinase: DNA binding and activation in the absence of Ku PNAS 1998 95: 525-530. Kunihiro Ohta, Alain Nicolas, Munenori Furuse, Akira Nabetani, Hideyuki Ogawa, and Takehiko Shibata Mutations in the MRE11, RAD50, XRS2, and MRE2 genes alter chromatin configuration at meiotic DNA double-stranded break sites in premeiotic and meiotic cells PNAS 1998 95: 646-651. William A. Cliby, Christopher J. Roberts, Karlene A. Cimprich, Cheri M. Stringer, John R. Lamb, Stuart L. Schreiber, and Stephen H. Friend Overexpression of a kinase-inactive ATR protein causes sensitivity to DNA-damaging agents and defects in cell cycle checkpoints EMBO J. 1998 17: 159-169. de Wit T, et al. Microinjection of Cre recombinase RNA induces site-specific recombination of a transgene in mouse oocytes. Nucleic Acids Res. 1998 Jan 15; 26(2): 676-678. Tsubouchi H, et al. A novel mre11 mutation impairs processing of double-strand breaks of DNA during both mitosis and meiosis. Mol Cell Biol. 1998 Jan; 18(1): 260-268. Shao Z, et al. Random-priming in vitro recombination: an effective tool for directed evolution. Nucleic Acids Res. 1998 Jan 15; 26(2): 681-683. Elliott B, et al. Gene conversion tracts from double-strand break repair in mammalian cells. Mol Cell Biol. 1998 Jan; 18(1): 93-101. From owner-recombination@net.bio.net Mon Feb 16 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Xiong Hu Newsgroups: bionet.molbio.recombination Subject: aligned sequences Date: 17 Feb 1998 11:02:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6ccms8$oom@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, I found this site from the internet and wonder if anyone would be able to help me with a question related to recombination. I am a Ph.D. student of statistics department at The Ohio State University. We have developed a method to test if two sets of nucleotide sequences are from the same phylogenetic tree. One of the potential application of this method is to test if recombination events has occured. However, we were not able to find some suitable data to apply our method. We wonder if you could help us out with this problem. What we are looking for is a set of aligned nucleotide sequences, with one being recombinant, and with the breakpoints identified since our method is not capable of picking out these points itself. It can only confirm (or deny) if the segments created by these points are significantly different. To make our method more effective, it is usually required to have at least 12 sequences of hundreds of sites. If you happen to have or know of any appropriate data, would you please inform us. Your advice is greatly appreciate. Thank you very much for your time and effort on this matter. Sincerely, Xiong Hu From owner-recombination@net.bio.net Mon Feb 16 22:00:00 1998 Path: biosci!biosci!not-for-mail From: "Dr.Narayana R.Isola" Newsgroups: bionet.molbio.recombination Subject: Sodium free DNA Date: 17 Feb 1998 11:03:20 -0800 Organization: Oak Ridge National Laboratory Lines: 7 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6ccmto$os8@net.bio.net> Reply-To: isolan@ornl.gov NNTP-Posting-Host: net.bio.net Does any one know how to get rid of sodium from DNA and get free acid? I would appreciate any input and references please Narayana R.Isola From owner-recombination@net.bio.net Tue Feb 17 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molbio.recombination Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 18 Feb 1998 04:47:32 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 235 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6cel94$2ma@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-recombination@net.bio.net Tue Feb 17 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: Gene Therapy Resource Pages from RECOM! Date: 18 Feb 1998 05:07:48 -0800 Organization: McGill Div. of Experimental Medicine Lines: 32 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6cemf4$4mi@net.bio.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net Hello! I am in the process of compiling a list of resources for GENE THERAPY and gene targeting. I will post these pages from the RECOM FAQ pages. http://www.medcor.mcgill.ca/EXPMED/DOCS/recomfaq.html If anyone has a list of bookmarks for the following areas please contact me by e-mail and I will post these with the GENE THERAPY RESOURCES: 1. Companies involved in Gene Therapy Trials 2. Companies that sell products used in gene targeting and gene delivery (ex. making vectors or transfection kits etc.) 3. Links to articles on ethics or science of Gene Therapy 4. Links to pages describing viruses involved in gene targeting (ex. adenovirus, herpes, retroviruses etc.) 5. Links to pages describing recombination mechanisms. 6. Links to pages showing structures of viruses and recombination proteins. All other links with relevance to Gene Therapy and Gene Targeting are appreciated. Thank you all in advance, Graham Dellaire Moderator RECOM dellaire@odyssee.net From owner-recombination@net.bio.net Wed Feb 18 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: Post Doc at NIH on Yeast Retrotransposons (H. Levine) Date: 18 Feb 1998 19:24:45 -0800 Organization: McGill Div. of Experimental Medicine Lines: 22 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6cg8lt$bc@net.bio.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net Yeast Retrotransposons Henry Levin, PhD A postdoctoral position is available to study the process of retrotransposition in the fission yeast, Schizosaccharomyces pombe. The retrotransposon Tf1 is a retrovirus model that is being used to identify yeast gene products that contribute to transposition. One gene product currently under investigation contributes specifically to the nuclear import of Tf1 while another participates in the deacetylation of chromatin. Laboratory of Eukaryotic Gene Regulation, NICHD, Building 6B, Room 2B-220. http://www.ncbi.nlm.nih.gov/Yeast/levin.html To request further information about this opportunity, or apply it online, visit this location on the NIH World Wide Web site: http://helix.nih.gov:8001/oe/laboratory/main.phtml?key=870899701 This is just a one of several current opportunities for postdoctoral training at the NIH. We also have openings for physicians in our clinical training programs and openings for tenure-track investigators. Check out the details, request further information and apply online today at the NIH Web site. From owner-recombination@net.bio.net Wed Feb 18 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: Post Doc Yeast DNA Replication (Joel Huberman, Roswell Park) Date: 18 Feb 1998 19:23:02 -0800 Organization: McGill Div. of Experimental Medicine Lines: 40 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6cg8im$t77@net.bio.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net Postdoctoral Position in Eukaryotic DNA Replication A postdoctoral research position is available starting in March, 1998, for studies on DNA replication origins and the initiation of DNA replication in the fission yeast, Schizosaccharomyces pombe. S. pombe replication origins are good models for those in animal cells, but are easier to study. Experience in biochemistry, molecular biology and/or yeast genetics is preferred, and a strong desire to participate in elucidating the mechanism of initiation of DNA replication in eukaryotic cells is required. Preference will be given to candidates who can begin work before May, 1998. Salary will be based on years of postdoctoral experience, according to the current NIH scale for postdoctoral trainees. The research will be carried out in the laboratory of Dr. Joel Huberman at Roswell Park Cancer Institute (RPCI) in Buffalo, New York. More can be learned about current research in Dr. Huberman's lab by accessing its WWW site at http://mcbio.med.buffalo.edu/jh.html. RPCI is one of the world's leading cancer research centers. It maintains strong programs in eukaryotic DNA replication, eukaryotic transcriptional regulation, and mammalian genetics. Located on the shore of Lake Erie close to Niagara Falls, Buffalo is an attractive small city with an unusually low cost of living and ample year-round recreational opportunities. Applicants should send a CV, a letter describing personal research interests, and the names, mail addresses, e-mail addresses, FAX and telephone numbers of at least 3 people who can provide references to: Dr. Joel A. Huberman Dept. of Molecular & Cellular Biology Roswell Park Cancer Institute Buffalo, NY 14263 716-845-3047 (phone) 716-845-8126 (FAX) huberman@acsu.buffalo.edu From owner-recombination@net.bio.net Wed Feb 18 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: Preprint:"VARIABLE-LENGTH GENOMES, OPERATORS OF TRANSPOSITION AND = Date: 18 Feb 1998 19:24:11 -0800 Organization: McGill Div. of Experimental Medicine Lines: 100 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6cg8kr$6a@net.bio.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net This announcement is being cross-posted to several newsgroups. Please, excuse the duplication. Dear All, The following paper is now available=20 as HTM Pages: http://www.iephb.ru/~spirov/te-tech/te-tech.htm and as ZIP-compressed PostScript file via anonymous ftp from: ftp://ftp.iephb.ru/pub/spirov/te_tech.zip (528 KB) These materials are submitted to "Evol.Comp.Journal".=20 Title: "VARIABLE-LENGTH GENOMES, OPERATORS OF TRANSPOSITION AND = PARASITIC MOBILE GENETIC ELEMENTS IN SIMULATIONS OF EVOLUTION" Authors: Alexander V. Spirov and Alexander B. Kazansky ABSTRACT The progress in the field of evolutionary self-learning algorithms is = primarily associated with the development of the set of operators, = realising the process of evolutionary search and adaptation. It is a = matter of fact, that the low "evolvability" of neo-darwinian population = with the classical set of evolutionary operators put a fundamental = limitation on the effectivity and universality of evolutionary = computations, based on this type of evolving system. The realisation of = this fact stimulates the constant interest of specialists in the field = of evolutionary computations to the achievements in evolutionary and = molecular biology. Godsends of nature can be adapted and applied to = evolutionary algorithms for perfection of the instrument for solving of = the hard optimisation problems. The creative role of viruses and = virus-like elements in evolution attracts nowadays a great interest of = evolutionary biologists as well as specialists in evolutionary = computations. In this work we put forward a new approach to evolutionary algorithms - = Transposable Elements (TE) technique. This technique was evaluated and = tested by the example of solving of biological, as well as classical = test behavioural problems (the John Muir's trail in ant's navigation = problem). The approach is based on the application of new algorithms, modelling = co-evolution of host population and a set of selfish/parasite mobile = genetic elements (transposons). These elements have ability to insertion = in the host's genome and to move from one genome site to another. = Besides, transposons are heritable and can be transmitted to the next = generations as well as to the other host during 'contacts'. Our TE technique presupposes the application of special new TE operators = of evolutionary computations along with classical ones. The TE operators = act on genes-strings of variable length just as known variable-length = operators of duplication, elimination and random addition. The TE technique is effective in cases, when traditional neo-darwinian = populations in framework of classical approaches loses the evolvability. = This technique gives selection advantage to functionally redundant, more = complex programs in the bottlenecks of evolutionary process. The developed method was successfully applied to the simulation of = biological evolution and to solving of ant's navigation test for = non-standard variant of trail. The possibilities for application of = proposed technique for solving GA-hard problems has being discussed as = well. KEYWORDS: self-organisation, evolution, transposons, genetic algorithms, = genetic networks, artificial life, evolutionary biology. (13 pages, 9 color illus.) PS: The PS-file was generated by WinWord 7 and tested by GhostScript viewer for Windows.=20 ________________________ Alexander V.Spirov (PhD) The Sechenov Institute of Evolutionary Physiology & Biochemistry, Thorez = Pr. 44, S.-Petersburg, 194223, Russia phone/fax +7 (812)552 3219; fax + 7 (812)552-3012 = http://www.iephb.ru/~spirov/; Email spirov@iephb.ru and Institute for High-Performance Computing & Data Bases, P.O. Box 71, = St.Petersburg 194291, RUSSIA From owner-recombination@net.bio.net Wed Feb 18 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Graham Dellaire Newsgroups: bionet.molbio.recombination Subject: Repost: Transfecting Jurkat Cells?? Date: 18 Feb 1998 19:36:30 -0800 Organization: McGill Div. of Experimental Medicine Lines: 11 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6cg9bu$1gv@net.bio.net> Reply-To: dellaire@odyssee.net NNTP-Posting-Host: net.bio.net Reposted Question on Transfection of Jurkats is there a preferred method for Transfections into Jurkats when one considers CaPo4,cationic lipids, electroporation,etc.,etc. I am primarily interested in transient transfecvtions for reporter gene assays. Thanks. "Prakash K. Rao" From owner-recombination@net.bio.net Thu Feb 19 22:00:00 1998 Path: biosci!biosci!not-for-mail From: shenderson@physci.ucla.edu (Scott Henderson) Newsgroups: bionet.molbio.recombination Subject: Host & Preparation of pJM17 or pBHG10 Date: 19 Feb 1998 18:08:53 -0800 Organization: UCLA Lines: 14 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6ciojl$frj@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone know of a *reliable* way to prepare and isolate the large plasmids (e.g., pJM17, pBHG10, pBHG11) used for adenoviral recombination. What host works well for these unstable plasmids? As of now my inclination is to use DH5 cells (old posts also suggest TG2 and SURE) and a "plating method" of maintaining plasmid-containing bacteria (Biotechniques 19(2): 202-4, 1995). Thanks in advance, Scott Henderson, PhD Physiological Science, UCLA ScottH@physci.ucla.edu