From owner-recombination@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!biosci!not-for-mail From: biota@onramp.net Newsgroups: bionet.molbio.recombination Subject: 100 New Tools Added to BioToolKit Date: 4 Jun 1998 13:27:09 -0700 Organization: OnRamp, http://www.onramp.net/ Lines: 30 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6l6vut$j2j@net.bio.net> Reply-To: biota@onramp.net NNTP-Posting-Host: net.bio.net The BioToolKit, an annotated directory of over 600 online molecular biology resources ( http://rampages.onramp.net/~biota) has recently added links to many new resources, including: ACUTS (Ancient Conserved Untranslated Sequences) dbCFC (Cytokine Family database) DNA Patent Database CD Guides (cell surface markers) MAR-Finder (Matrix Attachment Regions) In Situ PCR on Plant Material Vacuum Infiltration Transformation of Arabidopsis PPMdb (Plant Plasma Membrane database) MitBASE (comprehensive mitochondrial database) GenProtEC (E.coli gene relationships) The Interactive Fly WormPep (predicted proteins in C.elegans) WebMolecules VRML Player (analyzes your system, suggests viewers) 3-D Crunch (SWISS-MODEL predicts 50,000 structures) Atlas of Protein Topology Cartoons (simple 2-D diagrams) Movies of Protein Motions Protein Morphing Server PROMISE: Prosthetic Groups and Metal Ions in Protein Active Sites ADOPS: Associative Database of Protein Sequences NeuronDB Complete descriptions of these sites and links are found by clicking through to the BioToolKit host site from http://rampages.onramp.net ~biota. The BioToolKit database is designed for rapid page delivery, functionally organized, and Verity-searchable. From owner-recombination@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "Robert J. S. Reis" (by way of "G. Dellaire" Newsgroups: bionet.molbio.recombination Subject: Postdoctoral Position Available // C. elegans Longevity Genes Date: 4 Jun 1998 13:35:01 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 33 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6l70dl$k43@net.bio.net> NNTP-Posting-Host: net.bio.net ================================ Postdoctoral Position Available ================================ C. elegans Longevity Genes ================================ We are looking for a postdoctoral fellow, preferably with nematode experience +/or strong metazoan genetics background, to work on QTL mapping of longevity-determining genes in C. elegans. We have some related ongoing projects involving development of high-density, high-throughput mapping procedures in C. elegans (and also in mice & humans), and studies of long- lived ts dauer mutants to elucidate the underlying mechanisms. New ideas are always welcome. Reply by email to: rjreis@life.uams.edu or by regular mail to: Robert J. Shmookler Reis University of Arkansas for Medical Sciences VA Medical Center - Research 151 4300 West 7th Street Little Rock, AR 72205 If you want to look up some of our papers, please search under "Shmookler Reis" and also "Reis RJ" (NLM has indexed articles one way or the other, apparently at random!) From owner-recombination@net.bio.net Wed Jun 03 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Mark Cauchi Newsgroups: bionet.molbio.recombination Subject: Recombinant DNA Techniques Course Date: 4 Jun 1998 04:55:20 -0700 Organization: Monash University Lines: 9 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6l61v8$2rs@net.bio.net> Reply-To: Mark.Cauchi@med.monash.edu.au NNTP-Posting-Host: net.bio.net The Micromon Unit at the Department of Microbiology, Monash University in Melbourne, Australia will be running its Recombinant DNA Techniques Course between the 15-20 November, 1998. This is an introductory-intermediate level course which offers a skills-based training package. If you would like further information, details can be found on our web page at http://www.med.monash.edu.au/micro/department/dnacorse.htm From owner-recombination@net.bio.net Sat Jun 06 23:00:00 1998 Path: biosci!biosci!not-for-mail From: foleyk@zgi.com Newsgroups: bionet.molbio.recombination Subject: Cloning by recombination in Ecoli and yeast Date: 7 Jun 1998 15:14:08 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6lf3bg$dt4@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Group, Could anyone point the way towards references that address the following questions? 1. Is it feasible to introduce a linear DNA molecule into a non-linear plasmid by recombination in E. coli? The linear DNA will contain a selectable marker and be flanked by regions of homology with the target plasmid. If this works, how long do the flanking homologies have to be? What E.coli strains can be used? 2. How do bacteria and yeast compare with respect to the efficiency of this process? Thanks, Kevin Foley, Ph.D. Dept. of Genetics ZymoGenetics, Inc. Seattle, WA 98102 e-mail: foleyk@zgi.com From owner-recombination@net.bio.net Tue Jun 09 23:00:00 1998 Path: biosci!biosci!not-for-mail From: judelson@ucrac1.ucr.edu (Howard S. Judelson) Newsgroups: bionet.molbio.recombination Subject: Postdoc avail-Positional cloning//gene expression in oomycete fungus Date: 10 Jun 1998 14:12:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 42 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6lmsrs$a14@net.bio.net> NNTP-Posting-Host: net.bio.net POSTDOCTORAL POSITIONS AVAILABLE Riverside, California, USA Molecular genetics of the oomycete fungus, Phytophthora infestans Candidates are sought for two postdoctoral positions to study the molecular genetics of Phytophthora infestans, an oomycete fungus that causes the late blight diseases of potato and tomato. Positions are available in either of several projects. These include the positional cloning of fungicide resistance genes, the characterization of genes involved in sexual development and sporulation, and comparative studies of the structure and expression of mating type loci throughout the genus. Funding for these positions come from sources that include USDA and NSF grants. Additional information about the laboratory is available at: http://cnas.ucr.edu/~ppath/faculty/hj.html. Applicants should have experience in molecular biological techniques. Depending on the project, the experiments will involve methods such as genetic mapping, chromosome walking using BAC libraries, fungal transformation, cDNA cloning, expression analysis, and gene characterization; experience in such areas would be a plus but is not required. Riverside is located in Southern California, and is about 1-1.5 hours from Los Angeles and San Diego, mid-way between the ocean and the mountains. Please sent a curriculum vitae and the names of three references (including addresses and telephone numbers) to: Dr. Howard Judelson Department of Plant Pathology University of California Riverside, California 92521 USA email: judelson@ucrac1.ucr.edu fax: 909-787-4294 From owner-recombination@net.bio.net Thu Jun 11 23:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molbio.recombination Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Jun 1998 06:33:36 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 234 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6lrang$mck@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-recombination@net.bio.net Thu Jun 18 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "G. Dellaire" Newsgroups: bionet.molbio.recombination Subject: 12 New JOBS on EMJL (BIOMEDICAL JOB SITE!!!!) Date: 19 Jun 1998 07:39:10 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 79 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6mdt6e$kem@net.bio.net> NNTP-Posting-Host: net.bio.net New Jobs on the Experimental Medicine Job Listing (EMJL) http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html Montreal, Quebec, Canada Lady Davis Institute (McGill University) 1 Postdoctoral to study the role of protein phosphorylation in Alzheimer's Disease (H. Paudel).=20 Available Immediately Posted June 10th, 1998=20 Montreal Children's Hospital (McGill University) 1 Postdoctoral Position to characterize tissue- specific genes involved in third trimester lung development. and=20 1 Research Assistant position to study the role of Hox A5 in physiological functioning of the developing lung under the supervision of Dr. F. Kaplan (Montreal Children's Hospital Research Institute).=20 Deadline: July 7, 1998 Posted June 17, 1998=20 1 Postdoctoral Position to clone the antisense RNA involved in parental imprinting of IGF2R (Deadline June 24, 1998) and=20 1 Ph.D. Position to investigate the monoallelic expression of=20 p73 under the supervision of Dr. C. Polychronakos flexible deadline Posted June 14, 1998 -------------------------- United States Nurse Coordinatorat Allegheny University of the Health Sciences, Glenholden, PA.=20 Posted June 14, 1998=20 3 POSITIONS!at Allegheny University of the Health Sciences, Glenholden, PA.=20 Posted June 12, 1998=20 1 Postdoc Position to work on QTL mapping of longevity-determining=20 genes in C. elegans under the supervision of Dr. R.J. Shmookler Reis (University of Arkansas for Medical Sciences, Little Rock, AR.)=20 Available Immediately Posted June 4th/98=20 1 Post-Doc position to characterize novel genes involved in prostate cancer under the supervision of Dr. R. Sikes (University of Virginia Health Sciences Center, Charlottesville, VA) Posted June 4th/98=20 ---------------------- Switzerland 1 Postdoctoral Position to determine the mechanisms of neuronal and endocrine exocytosis under the supervision of Dr Claes Wollheim=20 and Dr Jochen Lang (Human Frontiers Project, 3 years support; Centre M=E9dical Universitaire, Geneva) Starting Date: October 1st, 1998 Posted: June 17, 1998 =3D=3D=3D=3D=3D=3D=3D=3DFIND THESE JOBS AND MORE AT EMJL=3D=3D=3D=3D=3D=3D= =3D=3D=3D=3D=3D=3D=3D=3D http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html From owner-recombination@net.bio.net Sat Jun 20 23:00:00 1998 Path: biosci!biosci!not-for-mail From: "G. Dellaire" Newsgroups: bionet.molbio.recombination Subject: Postdoc. Recombinational Mechanisms of telomere size control in Date: 21 Jun 1998 09:23:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 69 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6mjc20$iuu@net.bio.net> NNTP-Posting-Host: net.bio.net 1 Postdoctoral Position to study the recombinational mechanisms of telomere size control in yeast under the supervision of Dr. A.J. Lustig (Tulane University, New Orleans, Louisiana) Flexible Deadline Posted June 21, 1998 Dr. Arthur J. Lustig Tulane University Medical Center Tulane University New Orleans, Louisana, U.S.A. Project description: 1.An investigation of the mechanism of telomeric silencing in the yeast Saccharomyces cerevisiae and the inheritability of heterochromatin. Salary is commensurate with experience. 2.An investigation of a recombinationally-based mechanism of telomere size control in yeast Deadline is flexible Recent Publications 1.Liu, C. and Lustig, A. J. 1996. Genetic analysis of Rap1p/Sir3p interactions in telomeric and HML silencing in Saccharomyces cerevisiae. Genetics 143: 81-93. 2.Lustig, A., Liu, C., Zhang, C., and Hanish, J. P. 1996. Tethered Sir3p nucleates silencing at telomeres and internal loci in Saccharomyces cerevisiae. Mol. Cell. Biol. 16: 2483- 2495. 3.Li, B. and Lustig, A. J. 1996. A novel mechanism for telomere size control in Saccharomyces cerevisiae. Genes Dev. 10: 1310-1326. 4.Lustig, A. 1996. Methods in the analysis of telomere function in Saccharomyces cerevisiae. in Microbial Genome Methods (K. Adolph, ed.), CRC Press, Boca Raton, Florida, pp. 37-60 [invited review]. 5.Lustig, A. 1997. The identification of telomerase subunits: catalyzing telomere research. Trends Cell. Biol. 7: 299-302. 6.Lustig, A. 1998. Telomerases and non-LTR Retrotransposons: Different Means to a Common End? Current Biology 8: R161-R164. 7.Lustig, A. 1998. Mechanisms of silencing in Saccharomyces cerevisiae. Curr. Opin. Genet. and Dev. 8: 233-239. 8.Polotnianka, R.M., Liu, J. and Lustig, A.J.. 1998. The yeast Ku heterodimer is essential for protection against nucleolytic and recombinational activities. Current Biology, 8:in press. Positions: 1 Postdoctoral position Qualifications: Ph.D and significant experience in molecular biology and/or genetics. To Apply: For further information please see the EMJL job listings at: http://www.medcor.mcgill.ca/EXPMED/DOCS/jobsII.html#US From owner-recombination@net.bio.net Tue Jun 23 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Romina Gallo Newsgroups: bionet.molbio.recombination Subject: Non-homologous DNA integration (yeast) Date: 24 Jun 1998 07:34:45 -0700 Organization: ICGEB Lines: 10 Sender: daemon@net.bio.net Approved: dellaire@odyssee.net Distribution: world Message-ID: <6mr2q5$76u@net.bio.net> Reply-To: gallor@icgeb.trieste.it NNTP-Posting-Host: net.bio.net Hi there! Has anyone noticed if targeted integration in the yeast sequencing strain FY1679 is significantly occurring at non-homologous sites? Does anyone know what is the rate of failure to integrate an homologous region into its homologous site? Thank you much for your help. Romina Gallo